WO2009151610A3 - Flow cytometer apparatus for three dimensional diffraction imaging and related methods - Google Patents

Flow cytometer apparatus for three dimensional diffraction imaging and related methods Download PDF

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Publication number
WO2009151610A3
WO2009151610A3 PCT/US2009/003508 US2009003508W WO2009151610A3 WO 2009151610 A3 WO2009151610 A3 WO 2009151610A3 US 2009003508 W US2009003508 W US 2009003508W WO 2009151610 A3 WO2009151610 A3 WO 2009151610A3
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WO
WIPO (PCT)
Prior art keywords
flow cytometer
particle
related methods
fluid
dimensional diffraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/003508
Other languages
French (fr)
Other versions
WO2009151610A2 (en
Inventor
Xin-hua HU
Kenneth M. Jacobs
Jun O. Lu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
East Carolina University
Original Assignee
East Carolina University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by East Carolina University filed Critical East Carolina University
Priority to US12/997,186 priority Critical patent/US9013692B2/en
Priority to CN200980114507.7A priority patent/CN102037343B/en
Priority to EP09762906A priority patent/EP2300800A2/en
Publication of WO2009151610A2 publication Critical patent/WO2009151610A2/en
Publication of WO2009151610A3 publication Critical patent/WO2009151610A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0211—Investigating a scatter or diffraction pattern
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0211—Investigating a scatter or diffraction pattern
    • G01N2015/0222—Investigating a scatter or diffraction pattern from dynamic light scattering, e.g. photon correlation spectroscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N2015/025—Methods for single or grouped particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497—Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Computer Vision & Pattern Recognition (AREA)

Abstract

A flow cytometer assembly includes a fluid controller configured to form a hydrodynamically focused flow stream including an outer sheath fluid and an inner core fluid. A coherent light source is configured to illuminate a particle in the inner core fluid. A detector is configured to detect a spatially coherent distribution of elastically scattered light from the particle excited by the coherent light source. An analyzing module configured to extract a three-dimensional morphology parameter of the particle from a spatially coherent distribution of the elastically scattered light.
PCT/US2009/003508 2008-06-12 2009-06-11 Flow cytometer apparatus for three dimensional diffraction imaging and related methods Ceased WO2009151610A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/997,186 US9013692B2 (en) 2008-06-12 2009-06-11 Flow cytometer apparatus for three dimensional difraction imaging and related methods
CN200980114507.7A CN102037343B (en) 2008-06-12 2009-06-11 Flow cytometer apparatus for three dimensional diffraction imaging and related methods
EP09762906A EP2300800A2 (en) 2008-06-12 2009-06-11 Flow cytometer apparatus for three dimensional diffraction imaging and related methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6099308P 2008-06-12 2008-06-12
US61/060,993 2008-06-12

Publications (2)

Publication Number Publication Date
WO2009151610A2 WO2009151610A2 (en) 2009-12-17
WO2009151610A3 true WO2009151610A3 (en) 2010-03-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/003508 Ceased WO2009151610A2 (en) 2008-06-12 2009-06-11 Flow cytometer apparatus for three dimensional diffraction imaging and related methods

Country Status (4)

Country Link
US (1) US9013692B2 (en)
EP (1) EP2300800A2 (en)
CN (1) CN102037343B (en)
WO (1) WO2009151610A2 (en)

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Also Published As

Publication number Publication date
US9013692B2 (en) 2015-04-21
CN102037343B (en) 2013-10-02
US20110090500A1 (en) 2011-04-21
CN102037343A (en) 2011-04-27
EP2300800A2 (en) 2011-03-30
WO2009151610A2 (en) 2009-12-17

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