WO2010006333A3 - Quantification of enzyme activity by mass spectrometry using immobilized substrates - Google Patents

Quantification of enzyme activity by mass spectrometry using immobilized substrates Download PDF

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Publication number
WO2010006333A3
WO2010006333A3 PCT/US2009/050407 US2009050407W WO2010006333A3 WO 2010006333 A3 WO2010006333 A3 WO 2010006333A3 US 2009050407 W US2009050407 W US 2009050407W WO 2010006333 A3 WO2010006333 A3 WO 2010006333A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass spectrometry
quantification
enzyme activity
enzymes
immobilized substrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/050407
Other languages
French (fr)
Other versions
WO2010006333A2 (en
Inventor
Pedro Cutillas
Bart Vanhaesebroeck
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ludwig Institute for Cancer Research Ltd
Ludwig Cancer Research
Original Assignee
Ludwig Institute for Cancer Research Ltd
Ludwig Cancer Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Institute for Cancer Research Ltd, Ludwig Cancer Research filed Critical Ludwig Institute for Cancer Research Ltd
Priority to EP09795285A priority Critical patent/EP2313518A2/en
Priority to US13/003,731 priority patent/US20110281289A1/en
Publication of WO2010006333A2 publication Critical patent/WO2010006333A2/en
Anticipated expiration legal-status Critical
Publication of WO2010006333A3 publication Critical patent/WO2010006333A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The disclosure relates to methods of analyzing the enzymatic activity of enzymes in samples containing a plurality of enzymes, using mass spectrometry. Immobilized substrates are employed. Purified enzymes and enzymes from crude cell lysates can be analyzed using the disclosed methods.
PCT/US2009/050407 2008-07-11 2009-07-13 Quantification of enzyme activity by mass spectrometry using immobilized substrates Ceased WO2010006333A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09795285A EP2313518A2 (en) 2008-07-11 2009-07-13 Quantification of enzyme activity by mass spectrometry using immobilized substrates
US13/003,731 US20110281289A1 (en) 2008-07-11 2009-07-13 Quantification of enzyme activity by mass spectrometry using immobilized substrates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8001408P 2008-07-11 2008-07-11
US61/080,014 2008-07-11

Publications (2)

Publication Number Publication Date
WO2010006333A2 WO2010006333A2 (en) 2010-01-14
WO2010006333A3 true WO2010006333A3 (en) 2011-05-05

Family

ID=41507773

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/050407 Ceased WO2010006333A2 (en) 2008-07-11 2009-07-13 Quantification of enzyme activity by mass spectrometry using immobilized substrates

Country Status (3)

Country Link
US (1) US20110281289A1 (en)
EP (1) EP2313518A2 (en)
WO (1) WO2010006333A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0906698D0 (en) 2009-04-17 2009-06-03 Queen Mary & Westfield College Method for quantifying modified peptides
GB201204278D0 (en) 2012-03-09 2012-04-25 Queen Mary & Westfield College Method
KR101874501B1 (en) * 2013-03-15 2018-07-05 익스프레션 패톨로지, 인크. SRM Assay to Indicate Cancer Therapy
US10760113B2 (en) * 2015-07-10 2020-09-01 Regents Of The University Of Minnesota Kinase activity detection methods
GB201520178D0 (en) 2015-11-16 2015-12-30 Univ London Queen Mary Method
WO2019232225A1 (en) 2018-05-30 2019-12-05 Promega Corporation Broad-spectrum kinase binding agents
CN119023960B (en) * 2024-07-30 2025-11-18 中国人民解放军海军军医大学 A method for specific detection of asparaginase activity by immunomagnetic separation combined with substrate peptides using LC-MS/MS.
CN119534607A (en) * 2024-12-12 2025-02-28 中国科学技术大学 A portable and small-scale mass spectrometry method for rapid on-site bacterial identification based on multi-substrate-enzyme reaction

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007127767A2 (en) * 2006-04-28 2007-11-08 Ludwig Institute For Cancer Research Quantification of enzyme activity by mass spectrometry
US20080076676A1 (en) * 2006-02-27 2008-03-27 Korea Advanced Institute Of Science And Technology Novel construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080076676A1 (en) * 2006-02-27 2008-03-27 Korea Advanced Institute Of Science And Technology Novel construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof
WO2007127767A2 (en) * 2006-04-28 2007-11-08 Ludwig Institute For Cancer Research Quantification of enzyme activity by mass spectrometry

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
GRÜNINGER-LEITCH F ET AL: "Identification of beta-secretase-like activity using a mass spectrometry-based assay system.", NATURE BIOTECHNOLOGY JAN 2000 LNKD- PUBMED:10625394, vol. 18, no. 1, January 2000 (2000-01-01), pages 66 - 70, XP002626930, ISSN: 1087-0156 *
KIM Y-P ET AL: "Activity-based assay of matrix metalloproteinase on nonbiofouling surfaces using time-of-flight secondary ion mass spectrometry", ANALYTICAL CHEMISTRY 20080701 AMERICAN CHEMICAL SOCIETY US, vol. 80, no. 13, 1 July 2008 (2008-07-01), pages 5094 - 5102, XP002626931, DOI: DOI:10.1021/AC800299D *
KIM Y-P ET AL: "Protein kinase assay on peptide-conjugated gold nanoparticles by using secondary-ion mass spectrometric imaging", ANGEWANDTE CHEMIE - INTERNATIONAL EDITION 2007 WILEY-VCH VERLAG DE, vol. 46, no. 36, 2007, pages 6816 - 6819, XP002626932, DOI: DOI:10.1002/ANIE.200701418 *

Also Published As

Publication number Publication date
US20110281289A1 (en) 2011-11-17
WO2010006333A2 (en) 2010-01-14
EP2313518A2 (en) 2011-04-27

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