WO2010013771A1 - ユニバーサルな核酸プローブセットおよびその使用方法 - Google Patents

ユニバーサルな核酸プローブセットおよびその使用方法 Download PDF

Info

Publication number
WO2010013771A1
WO2010013771A1 PCT/JP2009/063562 JP2009063562W WO2010013771A1 WO 2010013771 A1 WO2010013771 A1 WO 2010013771A1 JP 2009063562 W JP2009063562 W JP 2009063562W WO 2010013771 A1 WO2010013771 A1 WO 2010013771A1
Authority
WO
WIPO (PCT)
Prior art keywords
probe
fluorescent
nucleic acid
probe set
utilization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/063562
Other languages
English (en)
French (fr)
Inventor
関口　勇地
尚宏 野田
宮田　亮
中村　和憲
蔵田　信也
常田　聡
英典 谷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
J-BIO 21 Corp
J-Bio21
National Institute of Advanced Industrial Science and Technology AIST
Nippon Steel Kankyo Engineering Co Ltd
Original Assignee
J-BIO 21 Corp
J-Bio21
National Institute of Advanced Industrial Science and Technology AIST
Nippon Steel Kankyo Engineering Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by J-BIO 21 Corp, J-Bio21, National Institute of Advanced Industrial Science and Technology AIST, Nippon Steel Kankyo Engineering Co Ltd filed Critical J-BIO 21 Corp
Priority to CA2735250A priority Critical patent/CA2735250A1/en
Priority to US12/737,567 priority patent/US20110212442A1/en
Priority to EP09803013A priority patent/EP2333059A4/en
Priority to JP2010522750A priority patent/JP5272230B2/ja
Publication of WO2010013771A1 publication Critical patent/WO2010013771A1/ja
Anticipated expiration legal-status Critical
Priority to US14/094,262 priority patent/US20140178876A1/en
Priority to US14/847,687 priority patent/US20150368712A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　本発明は、蛍光物質（ｄ）で標識されたヌクレオチド（ａ）を含むオリゴヌクレオチドからなる蛍光プローブ（Ａ）、及び、該蛍光プローブ（Ａ）にハイブリダイズする蛍光プローブ結合領域（ｂ１）と、標的核酸配列（Ｃ）にハイブリダイズする標的核酸結合領域（ｂ２）とを有するオリゴヌクレオチドからなるバインディングプローブ（Ｂ）からなり、上記蛍光物質（ｄ）が、グアニンと相互作用することにより蛍光キャラクターが変化する蛍光物質であり、上記蛍光プローブ（Ａ）を構成するヌクレオチドの少なくとも１つが、該プローブ（Ａ）と蛍光プローブ結合領域（ｂ１）との解離温度を上昇させる機能を有する人工ヌクレオチドであることを特徴とする核酸プローブセットである。 　上記本発明によれば、蛍光消光率の改善された、蛍光プローブとバインディングプローブとを有する核酸プローブセットを提供することができる。
PCT/JP2009/063562 2008-07-30 2009-07-30 ユニバーサルな核酸プローブセットおよびその使用方法 Ceased WO2010013771A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA2735250A CA2735250A1 (en) 2008-07-30 2009-07-30 Universal nucleic acid probe set and method for utilization thereof
US12/737,567 US20110212442A1 (en) 2008-07-30 2009-07-30 Universal nucleic acid probe set and method for utilization thereof
EP09803013A EP2333059A4 (en) 2008-07-30 2009-07-30 UNIVERSAL NUCLEIC ACID SET AND APPLICATIONS THEREFOR
JP2010522750A JP5272230B2 (ja) 2008-07-30 2009-07-30 ユニバーサルな核酸プローブセットおよびその使用方法
US14/094,262 US20140178876A1 (en) 2008-07-30 2013-12-02 Universal nucleic acid probe set and method for utilization thereof
US14/847,687 US20150368712A1 (en) 2008-07-30 2015-09-08 Universal nucleic acid probe set and method for utilization thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2008-196513 2008-07-30
JP2008196513 2008-07-30
JP2009142608 2009-06-15
JP2009-142608 2009-06-15

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/737,567 A-371-Of-International US20110212442A1 (en) 2008-07-30 2009-07-30 Universal nucleic acid probe set and method for utilization thereof
US14/094,262 Continuation US20140178876A1 (en) 2008-07-30 2013-12-02 Universal nucleic acid probe set and method for utilization thereof

Publications (1)

Publication Number Publication Date
WO2010013771A1 true WO2010013771A1 (ja) 2010-02-04

Family

ID=41610470

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/063562 Ceased WO2010013771A1 (ja) 2008-07-30 2009-07-30 ユニバーサルな核酸プローブセットおよびその使用方法

Country Status (5)

Country Link
US (3) US20110212442A1 (ja)
EP (1) EP2333059A4 (ja)
JP (2) JP5272230B2 (ja)
CA (1) CA2735250A1 (ja)
WO (1) WO2010013771A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012173274A1 (ja) 2011-06-16 2012-12-20 日鉄環境エンジニアリング株式会社 核酸測定用の核酸プローブ
JP2016096763A (ja) * 2014-11-20 2016-05-30 セイコーエプソン株式会社 核酸増幅方法
JP2021151246A (ja) * 2016-08-12 2021-09-30 Ｎｅｘｕｓｐｉｒａｌ株式会社 ゲノム編集方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
JP4851188B2 (ja) 2003-12-19 2012-01-11 日鉄環境エンジニアリング株式会社 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ
JP6629224B2 (ja) * 2014-11-06 2020-01-15 公立大学法人大阪 クランピングプローブ

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3437816B2 (ja) 1999-04-20 2003-08-18 環境エンジニアリング株式会社 核酸の測定方法
JP3963422B2 (ja) 2000-08-03 2007-08-22 日鉄環境エンジニアリング株式会社 核酸の測定方法
WO2008021466A1 (en) * 2006-08-16 2008-02-21 Yacoubian M D Vahe S Heart wire
JP2008182974A (ja) * 2007-01-31 2008-08-14 J-Bio 21 Corp 核酸プローブセットおよびその使用方法

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1736554B1 (en) * 1996-05-29 2013-10-09 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
CN1251609A (zh) * 1997-02-12 2000-04-26 尤金·Y·查恩 分析聚合物的方法和产品
AU9063398A (en) * 1997-09-12 1999-04-05 Exiqon A/S Oligonucleotide analogues
CA2304260C (en) * 1999-04-20 2009-03-24 Japan Bioindustry Association Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method and method for analyzing data obtained by the method
JP3985959B2 (ja) * 1999-04-20 2007-10-03 独立行政法人産業技術総合研究所 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法
EP2278030B1 (en) * 2000-04-10 2017-05-24 Taxon Biosciences, Inc. Methods for the survey and genetic analysis of populations
US20020081589A1 (en) * 2000-10-12 2002-06-27 Jing-Shan Hu Gene expression monitoring using universal arrays
EP1390530A2 (en) * 2001-01-05 2004-02-25 Genomicfx, Inc. Method for relative quantification of attached nucleic acids
US6558907B2 (en) * 2001-05-16 2003-05-06 Corning Incorporated Methods and compositions for arraying nucleic acids onto a solid support
EP1438435B1 (en) * 2001-08-31 2007-10-31 Datascope Investment Corp. Methods for blocking nonspecific hybridisations of nucleic acid sequences
AU2002351193A1 (en) * 2001-11-28 2003-06-10 Mj Bioworks Incorporated Parallel polymorphism scoring by amplification and error correction
ATE414177T1 (de) * 2002-09-30 2008-11-15 Hoffmann La Roche Oligonukleotide zur genotypisierung des thymidylat-synthase gens
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
EP1694869A2 (en) * 2003-11-10 2006-08-30 Investigen, Inc. Methods of preparing nucleic acid for detection
US8407013B2 (en) * 2005-06-07 2013-03-26 Peter K. Rogan AB initio generation of single copy genomic probes
US8076067B2 (en) * 2006-08-15 2011-12-13 Genetag Technology, Inc. Probe-antiprobe compositions and methods for DNA or RNA detection
CA2735246C (en) * 2008-07-30 2015-02-24 Nippon Steel Kankyo Engineering Co., Ltd. Nucleic acid probe set and method of using the same
EP2230312A1 (en) * 2009-03-19 2010-09-22 Helmholtz-Zentrum für Infektionsforschung GmbH Probe compound for detecting and isolating enzymes and means and methods using the same
FR2945545B1 (fr) * 2009-05-14 2011-08-05 Univ Aix Marseille Ii Methode de detection d'adn procaryote extrait d'un echantillon de selles
CN107419002A (zh) * 2009-09-01 2017-12-01 皇家飞利浦电子股份有限公司 用于微阵列选择的设备和方法
JP5901046B2 (ja) * 2010-02-19 2016-04-06 国立大学法人 千葉大学 ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント
US20130225623A1 (en) * 2010-10-27 2013-08-29 Mount Sinai School Of Medicine Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists
US20140248611A1 (en) * 2011-06-16 2014-09-04 Nippon Steel & Sumikin Eco-Tech Corporation Nucleic acid probe for assaying nucleic acids
US9273349B2 (en) * 2013-03-14 2016-03-01 Affymetrix, Inc. Detection of nucleic acids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3437816B2 (ja) 1999-04-20 2003-08-18 環境エンジニアリング株式会社 核酸の測定方法
JP3963422B2 (ja) 2000-08-03 2007-08-22 日鉄環境エンジニアリング株式会社 核酸の測定方法
WO2008021466A1 (en) * 2006-08-16 2008-02-21 Yacoubian M D Vahe S Heart wire
JP2008182974A (ja) * 2007-01-31 2008-08-14 J-Bio 21 Corp 核酸プローブセットおよびその使用方法

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
ANALYTICAL BIOCHEMISTRY, vol. 225, 1998, pages 32 - 38
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, 1997, pages 1143 - 1147
CROCKETT A.O. ET AL.: "Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides.", ANAL.BIOCHEM., vol. 290, no. 1, 2001, pages 89 - 97, XP002200212 *
KURATA S. ET AL.: "Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY((R)) FL-labeled probe or primer.", NUCLEIC ACIDS RES., vol. 29, no. 6, 2001, pages E34, XP002627527 *
LI X. ET AL.: "Universal molecular beacon-based tracer system for real-time polymerase chain reaction.", ANAL.CHEM., vol. 78, no. 22, 2006, pages 7886 - 7890, XP008136133 *
NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 303 - 308
NUCLEIC ACIDS RESEARCH, vol. 24, 1996, pages 4532 - 4535
See also references of EP2333059A4
TANI ET AL., ANALYTICAL CHEMISTRY
TANI H. ET AL.: "Universal quenching probe system: flexible, specific, and cost-effective real-time polymerase chain reaction method.", ANAL.CHEM., vol. 81, no. 14, 15 July 2009 (2009-07-15), pages 5678 - 5685, XP008136132 *
YANG C.J. ET AL.: "Synthesis and investigation of deoxyribonucleic acid/locked nucleic acid chimeric molecular beacons.", NUCLEIC ACIDS RES., vol. 35, no. 12, 2007, pages 4030 - 4041, XP008136134 *
YANG L. ET AL.: "A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids.", BMC MOL.BIOL., vol. 9, 4 June 2008 (2008-06-04), pages 54, XP021033494 *
ZHANG Y. ET AL.: "A novel real-time quantitative PCR method using attached universal template probe.", NUCLEIC ACIDS RES., vol. 31, no. 20, 2003, pages E123, XP002498258 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012173274A1 (ja) 2011-06-16 2012-12-20 日鉄環境エンジニアリング株式会社 核酸測定用の核酸プローブ
JP2016096763A (ja) * 2014-11-20 2016-05-30 セイコーエプソン株式会社 核酸増幅方法
JP2021151246A (ja) * 2016-08-12 2021-09-30 Ｎｅｘｕｓｐｉｒａｌ株式会社 ゲノム編集方法

Also Published As

Publication number Publication date
US20140178876A1 (en) 2014-06-26
JP5272230B2 (ja) 2013-08-28
EP2333059A4 (en) 2013-01-09
EP2333059A1 (en) 2011-06-15
CA2735250A1 (en) 2010-02-04
US20150368712A1 (en) 2015-12-24
JPWO2010013771A1 (ja) 2012-01-12
US20110212442A1 (en) 2011-09-01
JP2013150631A (ja) 2013-08-08

Similar Documents

Publication Publication Date Title
WO2008080029A3 (en) Methods and compositions for nucleic acid amplification
JP2013501508A5 (ja)
WO2007139723A8 (en) Chemicallymodified oligonucleotide primers for nucleic acid amplification
WO2008084405A3 (en) Circular chromosome conformation capture (4c)
WO2009135093A3 (en) Rnase-h-based assays utilizing modified rna monomers
WO2009060124A3 (en) Use of oligonucleotides with modified bases in hybridization of nucleic acids
WO2010013771A1 (ja) ユニバーサルな核酸プローブセットおよびその使用方法
JP2010535480A5 (ja)
WO2007064758A3 (en) Methods and systems for designing primers and probes
WO2007004057A3 (en) Chromosome conformation capture-on-chip (4c) assay
WO2009102896A3 (en) Isothermal nucleic acid amplification methods and compositions
WO2007106907A3 (en) Nucleic acid monomers with 2'-chemical moieties
WO2008109823A3 (en) Primers for melting analysis
JP2011502502A5 (ja)
WO2010040531A3 (en) Process of clean cloning
CN105658815A (zh) 用于添加适配体至核酸的方法及用于实施所述方法的组合物
JP2007532100A5 (ja)
KR101424192B1 (ko) 블로킹 올리고뉴클레오티드를 포함하는 핫스타트 ｐｃｒ용 조성물
WO2007011903A3 (en) Analyzing messenger rna and micro rna in the same reaction mixture
JP2015512251A5 (ja)
WO2011032034A3 (en) Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids
CA2532160A1 (en) Methods of genotyping using differences in melting temperature
WO2006074222A3 (en) Primer for nucleic acid detection
JP2016537005A5 (ja)
JP2005511055A5 (ja)

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09803013

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2010522750

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2735250

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2009803013

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 12737567

Country of ref document: US