WO2011035077A1

WO2011035077A1 - Novel compounds and therapeutic use thereof for protein kinase inhibition

Info

Publication number: WO2011035077A1
Application number: PCT/US2010/049199
Authority: WO
Inventors: Zhanggui Wu
Original assignee: Individual
Current assignee: Individual
Priority date: 2009-09-18
Filing date: 2010-09-17
Publication date: 2011-03-24
Anticipated expiration: 2012-03-18
Also published as: CA2774367A1; US20120232082A1; CN104628741A; EP2477496A4; US9487539B2; AU2010295492B2; AU2010295492A1; AU2016204867B2; IN2012DN03127A; BR112012006062A2; ES2481791T3; HK1170908A1; CN102711475A; AU2016204867A1; RU2012109220A; EP2826780A1; JP2013505254A; EP2826780B1; ES2587509T3; US8815863B2

Abstract

Novel compound having the following formula: (I) wherein Y is N, O, or S. Also disclosed are a pharmaceutical compositions comprising the same, methods for treating cancer using the same, and methods for the synthesis of the same. The novel compounds of the present invention are found to inhibit protein kinases, especially Checkpoint kinase Chk1/ Chk2.

Description

Novel Compounds and Therapeutic Use Thereof for Protein Kinase Inhibition

FIELD OF THE INVENTION

[001] The invention is related to methods and compositions for modulating the activities of check point kinase 1 (CHK1) and Checkpoint kinase 2 (CHK2), and receptor tyrosine kinase and non-receptor tyrosine kinase. More specifically, the present invention relates to the preparation and use of inhibitors of the above enzymes.

BACKGROUND OF THE INVENTION

[002] The etiology of cancer is extremely complex, and remains poorly understood.

What is known is that cancers are intimately related to both environmental factors as well as internal factors of the human body. About three quarters of cancers can be attributed to external, environmental causes. Cancers continue to pose great threat to humanity, and worldwide at least 5 million people die from cancer annually. Currently available methods of treatment, such as surgery, radiotherapy, chemotherapy all have their limitations.

[003] Of the available cancer treatment and prevention methods, chemical agents remain the most effective.

[004] One category of cancer chemotherapy agents are the inhibitors or antagonists of both receptor tyrosine kinases and non-receptor tyrosine kinases. Specific targets include vescular endothelial growth factor receptor (VEGFR), epidermal growth factor receptor (EGFR), HER2 (human epidermal growth factor receptor 2), SRC, JAK (Janus kinase) and TEK.

[005] Certain thieno-pyridazine compounds have been found to have anti-tumor activities. For example, WO2005105808 discloses such a compound as an IKK inhibitor:

In addition, WO2007124181 discloses compounds that are inhibitors of p38 protease, a type of tyrosine kinase:

Further, WO 03029241, WO 03028731 and WO2005066163 disclose similar compounds that are inhibitors of CHKL

[006] Nevertheless, there is a need for more anti-cancer compounds that are inhibitors of tyrosine kinases, especially CHK1/CHK2.

DESCRIPTION OF THE INVENTION

[007] The present invention provides novel inhibitors of certain protein kinases, especially cellular checkpoint kinases (CHK1 and CHK 2), and receptor tyrosine kinase and non-receptor tyrosine kinases, such as VEGFR (Vascular endothelial growth factor receptors), EGFR (epidermal growth factor receptor), HER2 (Human Epidermal growth factor Receptor 2), SRC kinases, JAK kinase and TEK kinases; and serine/threonine kinases such as MEK, JNK, c-MET, AKT, PIM, TIE and PLK.

[008] Checkpoint kinase 1 is an evolutionarily highly conserved protein kinase, and functions to control the progression of cell cycle at the S and G2/M check points. DNA damages activates Chkl, halting the cell cycle and causing the damaged DNA to be repaired. If the DNA damages are too extensive to repair, the cell dies such that the integrity and stability of the genome is maintained. Cancer cells devoid of or defective in Chkl display many defects in cell cycle control, including decreased cell division rate, lack of reaction to cell cycle check points, and increased sensitivity to DNA damages. Due to its functions in damage repair, Chkl plays an important role in the cancer ontology, cancer cell death, and cancer drug resistance.

[009] Research on checkpoints of cell cycle regulations revealed that inhibition of

CHK1 expression can reverse drug resistance or tolerance of cancer cells, thereby increasing the sensitivity of cancer cells to DNA damage therapy, and dramatically increasing the activities and effectiveness of anti-cancer agents. Most cancers have mutations in p53 that specifically eliminates Gl/S checkpoint, which can also be a basis for screening for specific anti-cancer agents. Inhibitors of Chkl kinase may be good candidates for specific anti-cancer agents or for enhancing the activities of other anti-cancer drugs, because Chkl kinase also regulates the G2/M checkpoint. Even though Chkl inhibitors themselves may not have strong anti-cancer activities, they may still be able to enhance the anticancer activities of other drugs if used in combination. [0010] The present invention provides novel protein kinase inhibitor compounds or pharmaceutically acceptable salts or derivatives thereof that have anti-cancer activities. The compounds of the present invention are inhibitors of cancer-related protein kinases such as CHK1 and CHK2, and can also increase or enhance the activities of other anti-cancer agents. The present invention also provides methods of preparing the compounds, pharmaceutical compositions comprising the compounds, and method of using the compounds in the preparation of pharmaceutical compositions for inhibiting cell multiplication or cell proliferation in warm blooded animals such as humans.

[0011] Cell cycle arrest-related or cell proliferation-related diseases that can be treated with compounds of the present invention or pharmaceutical compositions comprising the same include cancer (including solid tumors and leukemia), fibrosis and related diseases, psoriasis, rheumatoid arthritis,Kaposi sarcoma, acute and chronic renal diseases, atheroma, atherosclerosis,artery stenosis, autoimmune diseases, acute or chronic inflammation, bone dieseas, and eye diseases caused by retinal neo-angiogenesis.

[0012] The novel compounds of the present invention generally have Formula I described below.

(I)

where

Y = NH, O, or S,

R¹ = selected from following groups:

wherein X = CH₂, NH, S, or O,

R⁸ = -H, -NH₂, -OH, -N(R⁴, R⁵), -C(R⁴R⁵)_!_₇NR⁶R⁷, -C(R⁴R⁵)i_₇OR⁶, or -N(R⁴)NR⁵R⁶, wherein R⁴, R⁵, R⁶, R⁷ = H, alkyls (CrC₆), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S, and N, aryls (either unsubstituted or substituted aromatics), or heteroaromatics (either unsubstituted or substituted heteroaromatics),

R⁹, R¹⁰, R¹¹, R¹², and R¹³ = H, alkyls (Ci-C₆), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S or N; aryls (either unsubstituted and substituted aromatics), or heteroaromatics (either unsubstituted or substituted heteroaromatics),

R², is selected from a group consisting of H, OH, NH₂, OR¹⁴, NR¹⁴R¹⁵, alkyl, aryl, heteroaryl, cycloalkyl, arylalkyl, heterocyclyl, heterocyclylalkyl, alkenyl, and alkynyl,

wherein R¹⁴, R¹⁵ = H, alkyls (CrC₆), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S, N, aryls (either unsubstituted or substituted aromatics), or heteroaromatics (either unsubstituted or substituted heteroaromatics),

and

R is selected from a group consisting of H, alkyl, aryl, heteroaryl, cycloalkyl, arylalkyl, heterocyclyl, heterocyclylalkyl, alkenyl, and alkynyl. Specifically R is selected from the following groups:

wherein R , R , and R are selected from following groups: H; heteroatom such as F, CI, Br, I; alkyliCi-Cg); cycloalkyl (C₃-Cg) without or with substitutions, wherein a substitution is selected from the group consisting of alkyls (C Cg), cycloalkyls (C₃-Cg), aryls, heteroaryls; - OR¹⁹; -SR¹⁹; -NR¹⁹R²⁰; -S(0)R¹⁹; -S(0)₂R¹⁹; -S(0)₂NR¹⁹R²⁰; -C(0)NR¹⁹R²⁰; -N(R¹⁹)C(0)R²⁰; - N(R¹⁹)S(0)₂R²⁰; -N(R¹⁹)C(O)N(R²⁰R²¹); N(R¹⁹)C(0)OR²⁰; aryl with or without substitution, heteroaryl with or without substitution, aryalkyl with or without substitution, heterocyclyl with or without substitution, heteterocyclylalkyl with or without substitution; alkenyl with or without substitution, and alkynyl with or without substitution; where R 19 , R 20 , and R 21 are independently chosen from H, alkyl (C Cg), cycloalkyls (C₃-Cg), aryl with or without substitution, alkylaryl with or without substitutions, heteroaryl with or without substitution, or R¹⁶, R¹⁷, and R²¹ can be part of a fused ring containing 0-3 heteroatoms selected from N, O, and S.

[0013] The above compounds can be prepared as illustrated in the following general schemes:

Scheme 1:

Scheme 2:

[0014] In a preferred embodiment, the novel compounds of the present invention have a Formula II:

(Π) wherein,

R¹ is selected from following groups:

where X = CH₂, NH, S, or O;

R⁸ = -H, -NH₂, -OH, -N(R⁴, R⁵), -C(R⁴R⁵)_!_₇NR⁶R⁷, -C(R⁴R⁵)i_₇OR⁶, -

N(R⁴)NR⁵R⁶

R⁴, R⁵, R⁶, R⁷ = H, alkyls (Ci-C ), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S, N, aryls (selected from unsubstituted and substituted aromatics), or

heteroaromatics (selected from unsubstituted and substituted heteroaromatics)

R⁹, R¹⁰, R¹¹, R¹², R¹³ = H, alkyls (C^C⁶), cycloalkyls (C³-C⁸) with or without nuclear heteroatoms such as O, S, N, aryls (selected from unsubstituted and substituted aromatics) heteroaromatics (selected from from unsubstituted and substituted heteroaromatics),

R² is selected from a group consisting of H, OH, NH₂, OR¹⁴, NR¹⁴R¹⁵, alkyl, aryl, heteroaryl, cycloalkyl, arylalkyl, heterocyclyl, heterocyclylalkyl, alkenyl, alkynyl, wherein R¹⁴ and R¹⁵ are H, alkyl (CrC₆); cycloalkyl (C₃-C₈) without or with substitutions with heteroatoms such as O, S, and N; or a substituted or un- substituted aromatic ring,

R is selected from a group consisting of H, alkyl, aryl, heteroaryl, cycloalkyl, arylalkyl, heterocyclyl, heterocyclylalkyl, alkenyl, and alkynyl; preferably R is selected from the following groups:

where R , R and R are selected from following groups: H; heteroatom such as F, CI, Br, I; alkyl (C Cs); cycloalkyl (C3-C8) without and with substitutions: substitutions are selected from alkyls (Ci-C₈), cycloalkyls (C₃-C₈), aryls, and roaryls; -OR¹⁹; -SR¹⁹; -NR¹⁹R²⁰, -S(0)R¹⁹; - S(0)₂R¹⁹; -S(0)₂NR¹⁹R²⁰; -C(0)NR¹⁹R²⁰; -N(R¹⁹)C(0)R²⁰; -N(R¹⁹)S(0)₂R²⁰; - N(R¹⁹)C(O)N(R²⁰R²¹); -N(R¹⁹)C(0)OR²⁰; Aryl with or without substitution, heteroaryl with or without substitution, aryalkyl with or without substitution, heterocyclyl with or without substitution, heteterocyclylalkyl with or without substitution, alkenyl with or without substitution, alkynyl with or without substitution;

where R 19 , R 20 and R 21 chosen from H, alkyl (C Cg), cycloalkyls (C₃-Cg), aryl with or without substitution, alkylaryl with or witout substitutions, and heteroaryl with or without substitution; or R¹⁶, R¹⁷ and R¹⁸ can be part of ring which is fused containing 0-3 heteroatoms selected from N, O, and S.

[0015] The above compounds can be prepared as illustrated in the following general scheme:

[0016] Synthesis of the novel compounds according to the above schemes can be implemented using processes known to those skilled in the art, for example those disclosed in U.S. Pat. Publication No. US2009-0275585A1 and WO2005105808, which are incorporated herein by reference in their entirety.

[0017] The novel compounds of the present invention are inhibitors of protein kinases including CHK1 and CHK2, and can prevent DNA damage repair mechanism from arresting the cell cycle at the G2/M checkpoint. Thus these compounds have anti-proliferative (e.g. anticancer) activities, and can also be sued in combination with otehr anti-cancer agents, to enhance their anti-cancer effects. The compounds are thus useful as cancer therapeutic agents for treating humans and animals. The present invention further includes methods for making the novel compounds, pharmaceutical compositions comprising the compounds, the use of the compounds or its salts or prodrugs for the manufacture of pharmaceutical compositions, as well as methods of treatment using the pharmaceutical composition.

[0018] In another embodiment, the present invention provides a pharmaceutical composition comprising a compound described above and a pharmaceutically acceptable adjuvant or excipient, and a method for treating cancer by administering an effective amount of the pharmaceutical composition to a patient in need thereof. Administration of an "effective amount" or a "therapeutically effective amount" of a compound of the present invention means an amount that is useful, at dosages and for periods of time necessary to achieve the desired result. The therapeutically effective amount of a compound in accordance with the present invention may vary according to factors, such as the disease state, age, sex, and weight of the subject. Dosage regimens in the patient may be adjusted to provide the optimum therapeutic response. For example, several divided doses may be administered daily or the dose may be proportionally reduced as indicated by the exigencies of the therapeutic situation. In the context of the present invention, a "pharmaceutically acceptable salt," refer to salts prepared from pharmaceutically acceptable, non-toxic acids.

[0019] The pharmaceutical compositions according to the invention can be present and administered as liquid, semi-solid or solid medicament forms and in the form of e.g. injection solutions, drops, juices, syrups, suspensions, sprays, granules, tablets, pellets, patches, capsules, plasters, suppositories, ointments, creams, lotions, gels, emulsions or aerosols, and comprise a compound of the present invention, and pharmaceutical auxiliary substances according to the galenical form, such as e.g. carrier materials, fillers, solvents, diluents, surface-active substances, dyestuffs, preservatives, disintegrating agents, anti-friction agents, lubricants, flavorings and/or binders. These auxiliary substances can be, for example: water, ethanol, 2- propanol, glycerol, ethylene glycol, propylene glycol, polyethylene glycol, polypropylene glycol, glucose, fructose, lactose, sucrose, dextrose, molasses, starch, modified starch, gelatine, sorbitol, inositol, mannitol, microcrystalline cellulose, methylcellulose, carboxymethylcellulose, cellulose acetate, shellac, cetyl alcohol, polyvinylpyrrolidone, paraffins, waxes, naturally occurring and synthetic gums, acacia gum, alginates, dextran, saturated and unsaturated fatty acids, stearic acid, magnesium stearate, zinc stearate, glyceryl stearate, sodium lauryl sulfate, edible oils, sesame oil, coconut oil, ground nut oil, soya bean oil, lecithin, sodium lactate, polyoxyethylene and -propylene fatty acid esters, sorbitan fatty acid esters, sorbic acid, benzoic acid, citric acid, ascorbic acid, tannic acid, sodium chloride, potassium chloride, magnesium chloride, calcium chloride, magnesium oxide, zinc oxide, silicon dioxide, titanium oxide, titanium dioxide, magnesium sulfate, zinc sulfate, calcium sulfate, potash, calcium phosphate, dicalcium phosphate, potassium bromide, potassium iodide, talc, kaolin, pectin, crosspovidone, agar and bentonite. The choice of auxiliary materials and the amounts thereof to be employed depend on whether the medicament is to be administered orally, perorally, subcutaneously, parenterally, intravenously, intraperitoneally, intradermally, intramuscularly, intranasally, buccally, rectally or locally, for example to infections on the skin, the mucous membranes and the eyes. Formulations in the form of tablets, coated tablets, capsules, granules, drops, juices and syrups are suitable, inter alia, for oral administration, and solutions, suspensions, easily reconstitutable dry formulations and sprays are suitable for parenteral, topical and inhalatory administration. A compound according to the invention in a depot in dissolved form or in a patch, optionally with the addition of agents which promote penetration through the skin, are suitable formulations for percutaneous administration. Formulation forms which can be used orally or percutaneously can release the compound according to the invention in a delayed or controlled manner.

[0020] The medicaments and pharmaceutical compositions according to the invention are prepared with the aid of agents, devices, methods and processes which are well-known in the prior art of pharmaceutical formulation, such as are described, for example, in Remington's Pharmaceutical Sciences, 18th ed., Mack Publishing Co., Easton, Pa. (1990), in particular in part 8, sections 76 to 93.

[0021] Thus for a solid formulation, such as a tablet, the active compound of the medicament, can be mixed with a pharmaceutical carrier, e.g. conventional tablet constituents, such as maize starch, lactose, sucrose, sorbitol, talc, magnesium stearate, dicalcium phosphate or gum, and pharmaceutical diluents, such as e.g. water, in order to form a solid preformulation composition which comprises a compound according to the invention or a pharmaceutically acceptable salt thereof in homogeneous distribution. Homogeneous distribution here is understood as meaning that the active compound is distributed uniformly over the entire preformulation composition, so that this can easily be divided into unit dose forms of the same action, such as tablets, pills or capsules. The solid preformulation composition is then divided into unit dose forms. The tablets or pills of the medicament according to the invention or of the compositions according to the invention can also be coated, or compounded in another manner in order to provide a dose form with delayed release. Suitable coating compositions are, inter alia, polymeric acids and mixtures of polymeric acids with materials such as e.g. shellac, cetyl alcohol and/or cellulose acetate.

[0022] The amount of active compound to be administered to the patient varies and depends on the weight, age and disease history of the patient, as well as on the mode of administration, the indication and the severity of the disease. A range of does, for example 0.1 to 5,000 mg/kg, in particular 1 to 500 mg/kg, preferably 2 to 250 mg/kg of body weight of a compound according to the invention are usually administered.

[0023] The pharmaceutical composition of the present invention may be administered enterally (such as orally or via rectal administration), externally, or parenterally e.g. via injection. Suitable formulations include tablets (such as conventional tablets, buccal tablets, sublingual tablet, oral cavity patch, chewable tablet, effervescent tablet, vaginal tablet, vaginal effervescent tablet, sustained-release tablet, controlled release tablet, enteric coated tablet, buccal rapid-release tablet), capsules (hard capsules, soft capsules, sustained-release capsules, controlled-release capsules, enteric-coated capsules, etc), pills (dripping pills, sugar coated pills, pellets), oral liquid (oral solution, oral suspension, oral emulsion, etc), granules (suspension granules, soluble granules, effervescent granules, gastro-resistant granules, sustained-release granules, controlled-release granules, etc), injection (injectable solution, injectable emulsion, injectable suspension), intravenous infusion, powder for injection, concentrated solution for injection, implants, etc, and other medicament form such as suppositories, aerosol, aerosol powder, spray, gel, pellicles, patches, etc.

[0024] Compounds of the present invention may be incorporated into biodegradable polymers allowing for sustained release of the compound, the polymers being implanted in the vicinity of where drug delivery is desired, for example, at the site of a tumor or implanted so that the compound is slowly released. The biodegradable polymers and their use are described, for example, in detail in Brem et al., J. Neurosurg. 74:441-446(1991). Osmotic mini pumps may also be used to provide controlled delivery.

[0025] Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose the therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio LD50/ED50. Compounds which exhibit large therapeutic indices are preferred. The data obtained from these cell culture assays and animal studies can be used in formulating a range of dosage for use in human. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized.

[0026] Pharmaceutical composition of the present invention is suitable for treating cancers and the related diseases, and may be used alone or in combination with other anticancer drugs. An ordinarily skilled person in the art will be able to determine the suitable dosage for the treatment, depending on the types of disease to be treated, the formulation and the conditions of the patient. [0027] The compounds of this invention can be used to prevent or treat abnormal cell proliferation, especially those found in tumors or cancers, including lung cancer, liver cancer, leucocythaemia, osteocarcinoma, pancreas cancer, skin cancer, melanoma, metrocarcinoma, oophoroma, rectal carcinoma, gastric carcinoma, colon cancer, breast carcinoma, salpinx carcinoma, endometrium carcinoma, cervix carcinoma, vagina carcinoma, carcinoma of vulva, esophagus carcinoma, small intestine carcinoma, incretion carcinoma, soft tissue sarcoma, urethra carcinoma, prostatic cancer, lymphocytoma, bladder cancer, nephridium cancer, tumors of vertebral column, tumors in the neuroglia of the brain, and pituitary adenoma.

[0028] The pharmaceutical composition of the present inveniton may also be used for the prevention or treatment of autoimmune diseases, inflammation, nerve system diseases, and cardiovascular diseases. Especially, the pharmaceutical compositions of the present invention may be used to treat cell cycle-related or cell proliferation related diseases.

EXAMPLES

[0029] Example 1 Synthesis of Compounds of Compound (S)-2-(3-fluorophenyl)-4-

(piperidin-3-ylamino)thieno[2,3-d]pyridazine-7-carboxamide

[0030] Following the following scheme 3, Compound (S)-2-(3-fluorophenyl)-4-

(piperidin-3-ylamino)thieno[2,3-d]pyridazine-7-carboxamide was synthesized:

[0031] Step 1

[0032] The LDA (20 mmol) was added to the solution of thiophene (5.34 g, 20 mmol) and diethyl oxalate in THF (100 mL) at -78 °C dropwise. After the addition, the resulting mixture was allowed to warm to 0 °C and stirred at 0 °C for 30 min. HCl (IN) was added to quench the reaction and extracted with EtOAc. The organics was dried over Na₂S0₄ and concentrated. The residue was purified with column gave the product as yellowish solid (7.07 g). HPLC-MS t_R = 2.39 min (UV_{254 nm}); mass calculated for formula C₁₇H₁₅F0₅S 350.0, observed LCMS m/z 351.0 (M+H).

[0033] Step 2

[0034] The ketone from step 1 (7.07 g, 19.2 mmol) was dissolved in ethanol (50 mL) and hydrazine (1 mL) was added. The mixture was heated up to 60 °C and stirred for 10 min. Then, the mixture was cooled to room temperature and stirred for another 30 min. The solid was collected by filtration and washed with small amount cold ethanol. The solid was dried under air gave the pure product (6.17 g) as white solid. HPLC-MS tR = 1.88 min (UV_{254 nm}); mass calculated for formula C₁₅HnFN₂0₃S 318.0, observed LCMS m/z 319.0 (M+H).

[0035] Step 3 [0036] The heterocyclic compound from step 2 (2.8 g, 8.8 mmol) was added to P(0)C13

(20 mL) and the mixture was heated up to 90 °C and stirred at that temperature for 3 hours. The solvent was removed under reduced pressure and the residue was taken up with EtOAc. The solid was filtered off and the organics was concentrated and purified with column (silica gel) gave the product (2.31 g) as yellowish solid. HPLC-MS t_R = 2.40 min (UV_{254 nm}); mass calculated for formula C₁₅HioClFN₂0₂S 336.0, observed LCMS m/z 337.0 (M+H).

[0037] Step 4

[0038] The chloro-compound (336 mg, 1.0 mmol) was dissolved in NMP (5 mL) and

K₂CO₃ (272 mg, 2.0 mmol) and (S)-tert-butyl 3-aminopiperidine-l-carboxylate (200 mg, 1.0 mmol) was added. The mixture was heated up to 80 °C and stirred for 3 hour. After cooling to room temperature, the mixture was diluted with EtOAc and washed with water, brine and dried over Na₂S0₄. After concentration, the crude was purified with column give the product (357 mg). HPLC-MS t_R = 2.09 min (UV_{254 nm}); mass calculated for formula C₂₅H₂₉FN₄0₄S 500.2, observed LCMS m/z 501.1 (M+H).

[0039] Step 5

[0040] The (S)-ethyl 4-(l-(tert-butoxycarbonyl)piperidin-3-ylamino)-2-(3- fluorophenyl)thieno[2,3-d]pyridazine-7-carboxylate (50 mg, 0.1 mmol) was mixed with ammonia (4 mL) in a sealed tube at room temperature. The mixture was heated up to 80 °C and stirred overnight. Then the solvent was removed by concentration. The crude product (S)-tert- butyl 3-(7-carbamoyl-2-(3-fluorophenyl)thieno[2,3-d]pyridazin-4-ylamino)piperidine-l- carboxylate was used in the next step directly without further purification. HPLC-MS t_R = 1.75 min (UV_{254 nm}); mass calculated for formula C^H^FNsOsS 471.2, observed LCMS m/z 472.2 (M+H).

[0041] Step 6

[0042] The crude product from step 5 (S)-tert-butyl 3-(7-carbamoyl-2-(3- fluorophenyl)thieno[2,3-d]pyridazin-4-ylamino)piperidine-l-carboxylate was treated with HC1 (4N in dioxane, 3 mL) at room temperature for 30 min. Then the solvent was removed under reduced pressure and the residue was purified with HPLC to obtain the final compound (S)-2- (3-fluorophenyl)-4-(piperidin-3-ylamino)thieno[2,3-d]pyridazine-7-carboxamide. HPLC-MS t_R = 1.27 min (UV_{254 nm}); mass calculated for formula CigHigFNsOS 371.1, observed LCMS m/z 372.1 (M+H).

[0043] Example 2

[0044] Synthesis of Compound 2-(3-fluorophenyl)-4-(piperidin-3-yloxy)thieno[2,3- d]pyridazine-7-carboxamide

[0045] Ethyl 4-chloro-2-(3-fluorophenyl)thieno[2,3-d]pyridazine-7-carboxylate has been made in example 1 step 3.

[0046] Step 1

[0047] iert-Butyl 3-hydroxypiperidine-l-carboxylate (100 mg, 0.5 mmol) was dissolved in dry NMP (5 mL) and NaH (20 mg, 60% in oil, 0.5 mmol) was added carefully. The mixture was stirred at room temperature for 10 min. Then, ethyl 4-chloro-2-(3-fluorophenyl)thieno[2,3- d]pyridazine-7-carboxylate (130 mg, 0.5 mmol) was added. The mixture was heated up to 80 °C and stirred for 30 min. After cooling to room temperature, EtOAc was added to dilute the reaction and washed with water and brine. The organics was dried over Na₂S0₄ and filtered, concentrated. The crude product was purified with column (silica gel) to give the product ethyl 4-(l-(tert-butoxycarbonyl)piperidin-3-yloxy)-2-(3-fluorophenyl)thieno[2,3-d]pyridazine-7- carboxylate (151 mg). HPLC-MS t_R = 2.17 min (UV_{254 nm}); mass calculated for formula C₂₅H₂₈FN₃0₅S 501.2, observed LCMS m/z 502.2 (M+H).

[0048] Step 2 [0049] The compound tert-butyl 3-(7-carbamoyl-2-(3-fluorophenyl)thieno[2,3- d]pyridazin-4-yloxy)piperidine-l-carboxylate was prepared with the same conditions described in example 1 step 5. HPLC-MS IR = 1.88 min (UV_{254 nm}); mass calculated for formula

C₂₃H₂₅FN₄O₄S 472.2, observed LCMS m/z 473.1 (M+H).

[0050] Step 3

[0051] The compound 2-(3-fluorophenyl)-4-(piperidin-3-yloxy)thieno[2,3- d]pyridazine-7-carboxamide was prepared with the same conditions described in example 1 step 6. HPLC-MS t_R = 1.33 min (UV₂₅4 nm); mass calculated for formula C18H17FN4O2S 372.1, observed LCMS m/z 373.1 (M+H).

[0052] Example 3

[0053] Synthesis of Compound 2-(3-fluorophenyl)-4-(piperidin-3-ylthio)thieno[2,3- d]pyridazine-7-carboxamide was prepared

[0054] Followed the same procedure described in the example 2, the following compound 2-(3-fluorophenyl)-4-(piperidin-3-ylthio)thieno[2,3-d]pyridazine-7-carboxamide was prepared. HPLC-MS tR = 1.35 min (UV254 _nm); mass calculated for formula C₁8H₁7FN₄0S₂ 388.1, observed LCMS m/z 389.0 (M+H).

[0055] Example 4 Synthesis of Compound 2-(3-fluorophenyl)-4-(piperidin-3- ylmethyl)thieno[2,3-d]pyridazine-7-carboxamide

[0056] Step 1

[0057] Under Ar, the compound tert-butyl 3-methylenepiperidine-l-carboxylate (600 mg, 3.0 mmol) was dissolved in dry THF (15 mmol) and 9-BBN (3.0 mmol) was added dropwise. The resulting mixture was stirred at room temperature for 2 hour. The solution was added to the mixture of the chloro-compound ethyl 4-chloro-2-(3-fluorophenyl)thieno[2,3- d]pyridazine-7-carboxylate (260 mg, 1.0 mmol) was mixed with Pd(dppf)Ci₂ (40 mg, 0.05 mmol, K₃PO₄ (636 mg, 3.0 mmol), in dioxane (10 mL with 1 ml water) under Ar. The resulting mixture was heated at 90 °C and stirred overnight. After cooled to room temperature, the mixture was diluted with EtOAc (60 mL) and filtered through celite. After concentration, the crude was purified with column (silica gel, 0-30% EtOAc/Hexane) gave the product ethyl 4- ((l-(tert-butoxycarbonyl)piperidin-3-yl)methyl)-2-(3-fluorophenyl)thieno[2,3-d]pyridazine-7- carboxylate (266 mg). HPLC-MS t_R = 2.38 min (UV_{254 nm}); mass calculated for formula C₂₆H₃₀FN₃O₄S 499.2, observed LCMS m/z 500.3 (M+H).

[0058] Step 2

[0059] The compound tert-butyl 3-((7-carbamoyl-2-(3-fluorophenyl)thieno[2,3- d]pyridazin-4-yl)methyl)piperidine-l-carboxylate was prepared with the same conditions described in example 1 step 5. HPLC-MS tR = 1.95 min (UV_{254 nm}); mass calculated for formula C₂₄H₂₇FN₄O₃S 470.2, observed LCMS m/z 471.2 (M+H).

[0060] Step 3

[0061] The compound 2-(3-fluorophenyl)-4-(piperidin-3-ylmethyl)thieno[2,3- d]pyridazine-7-carboxamide was prepared with the same conditions described in example 1 step 6. HPLC-MS t_R = 1.39 min (UV_{254 nm}); mass calculated for formula C₁₉H₁₉FN₄OS 370.1, observed LCMS m/z 371.1 (M+H).

[0062] Example 4

[0063] Similarly, following Scheme 3 described above, the following compounds are synthesized.

2-(4-Chloro-phenyl)-4-(piperidin-3-yloxy) 2-(4-Chloro-phenyl)-4-(piperidin-3-ylsulfanyl) -thieno[2,3-rf]pyridazine-7-carboxylic acid amide -thieno[2,3-rf]pyridazine-7-carboxylic acid amide

[0064] Example 5 Synthesis of Compounds of Formula II:

[0065] The compounds of the present invention are synthesized as follows: In the presence of a base, a compound of Formula A is treated with dialkyl oxalate, to obtain a compound of formula B:

B

Compound B is treated with hydrazine, to form a compound of Formula C:

C which is treated with phosphoric trichloride (POCI₃), resulting in a compound of Formula D

D

Compound D is then reacted with amine NH₂R¹, to form a compound of Formula E,

E

which is then reacted with another amine, NH₂R , followed by the removal of the protective group on R¹, and a treatment with base, resulting in a compound of Formua 1, or a

pharmaceutically acceptable salt thereof.

[0066] In the above synthesis scheme, R, R¹, R² and R³ are as defined previously

Preferably, R is C_1-4 alkyl; R¹ is a saturated or unsatured 5- or 6-membered rign containing N,

S, or O, or a stereoisomer thereof, R 2 is independently H, or a C_1-4 alkyl; and R 1 is a singly or doubly halogen substituted benzyl, which substitution may occur at any position.

[0067] Following the above method, the compounds listed below are synthesized:

1. 2-(4-fluorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2. 2-(4-chlorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

3. 2-(4-bromophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

4. 2-(4-fluorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

5. 2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 6. 2-(4-bromophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

7. 2-(4-fluorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

8. 2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

9. 2-(4-bromophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

10. 2-(4-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

11. 2-(4-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

12. 2-(4-chlorophenyl)-4-(S-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

13. 2-(4-chlorophenyl)-4-(R-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

14. 2-(4-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

15. 2-(4-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

16. 2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

17. 2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N,N- dimethyl-carboxylic acid amide

18. 2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

19. 2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide 20. 2-(4-fluorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

21. 2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

22. 2-(4-bromophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

23. 2-(4-fluorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

24. 2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

25. 2-(4-bromophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

26. 2-(4-fluorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

27. 2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

28. 2-(4-bromophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

29. 2-(4-chlorophenyl)-4-(2-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

30. 2-(4-chlorophenyl)-4-(S-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

31. 2-(4-chlorophenyl)-4-(R-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

32. 2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

33. 2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide 34. 2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N-meth carboxylic acid amide

35. 2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

36. 2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

37. 2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

38. 2-(4-fluorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

39. 2-(4-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

40. 2-(4-bromophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

41. 2-(4-fluorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

42. 2-(4-chlorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

43. 2-(4-bromophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

44. 2-(4-fluorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

45. 2-(4-chlorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

46. 2-(4-bromophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

47. 2-(4-chlorophenyl)-4-(2-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

48. 2-(4-chlorophenyl)-4-(4-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

49. 2-(4-chlorophenyl)-4-(S-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

50. 2-(4-chlorophenyl)-4-(R-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 51. 2-(4-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

52. 2-(4-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

53. 2-(4-chlorophenyl)-4-(3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

54. 2-(4-chlorophenyl)-4-(3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

55. 2-(4-chlorophenyl)-4-(3-pyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

56. 2-(4-chlorophenyl)-4-(3-pyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

57. 2-(4-fluorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

58. 2-(4-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

59. 2-(4-bromophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

60. 2-(4-fluorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

61. 2-(4-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

62. 2-(4-bromophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

63. 2-(4-fluorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

64. 2-(4-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

65. 2-(4-bromophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

66. 2-(4-chlorophenyl)-4-(2-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

67. 2-(4-chlorophenyl)-4-(S-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

68. 2-(4-chlorophenyl)-4-(R-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

69. 2-(4-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide 70. 2-(4-chlorophenyl)-4-(3-pyrrol-amino)-thieno[

acid amide

71. 2-(4-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

72. 2-(4-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

73. 2-(4-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

74. 2-(4-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

75. 2-(3-fluorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

76. 2-(3-chlorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

77. 2-(3-bromophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

78. 2-(3-fluorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

79. 2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

80. 2-(3-bromophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

81. 2-(3-fluorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

82. 2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

83. 2-(3-bromophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

84. 2-(3-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 85. 2-(3-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

86. 2-(3-chlorophenyl)-4-(S-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

87. 2-(3-chlorophenyl)-4-(R-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

88. 2-(3-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

89. 2-(3-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

90. 2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

91. 2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N,N- dimethyl-carboxylic acid amide

92. 2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

93. 2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide

94. 2-(3-fluorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

95. 2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

96. 2-(3-bromophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

97. 2-(3-fluorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

98. 2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 99. 2-(3-bromophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

100. 2-(3-fluorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

101. 2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

102. 2-(3-bromophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

103. 2-(3-chlorophenyl)-4-(2-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

104. 2-(3-chlorophenyl)-4-(S-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

105. 2-(3-chlorophenyl)-4-(R-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

106. 2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

107. 2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N- dimethyl-carboxylic acid amide

108. 2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

109. 2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N,N- dimethyl-carboxylic acid amide

110. 2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

111. 2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N,N- dimethyl-carboxylic acid amide

112. 2-(3-fluorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 113. 2-(3-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

114. 2-(3-bromophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

115. 2-(3-fluorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

116. 2-(3-chlorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

117. 2-(3-bromophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

118. 2-(3-fluorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

119. 2-(3-chlorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

120. 2-(3-bromophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

121. 2-(3-chlorophenyl)-4-(2-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

122. 2-(3-chlorophenyl)-4-(4-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

123. 2-(3-chlorophenyl)-4-(S-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

124. 2-(3-chlorophenyl)-4-(R-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

125. 2-(3-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

126. 2-(3-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide 127. 2- (3-chlorophenyl)-4-(3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

128. 2- (3-chlorophenyl)-4-(3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

129. 2- (3-chlorophenyl)-4-(3-pyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

130. 2- (3-chlorophenyl)-4-(3-pyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

131. 2- (3-fluorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

132. 2- (3-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

133. 2- (3-bromophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

134. 2- (3-fluorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

135. 2- (3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

136. 2- (3-bromophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

137. 2- (3-fluorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

138. 2- (3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

139. 2- (3-bromophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

140. 2- (3-chlorophenyl)-4-(2-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

141. 2- (3-chlorophenyl)-4-(S-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

142. 2- (3-chlorophenyl)-4-(R-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 143. 2-(3-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

144. 2-(3-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

145. 2-(3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

146. 2-(3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

147. 2-(3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide, and

148. 2-(3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide.

[0068] Example 6 Formulations

[0069] Pharmaceutical preparations for delivery by various routes are formulated as shown in the following. "Active ingredient" or "Active compound" as used in the following means one or more of the compounds of Formula I.

[0070] 1. Parenteral injection. Formulation: Active ingredient, 50 g; Sodium chloride, 2250g; Water for injection add to 250,000 ml, to make 1,000 bottles.

[0071] Preparation: The active ingredient is dissolved in a portion of water for injection; a sufficient quantity of sodium chloride is then added with stirring to make the solution isotonic. Its pH is adjusted to 4.0 to 5.0. Activated carbon (250g)is added to for 30 min and then removed using a filter rod. The mixture is then bottled in 250 ml/ after filtration with Titanium Rod until the solution is clear, which is then sterilized in a 115°C water bath for 30 min.

[0072] 2. Pellet. Formulation: Active ingredient, 50 g; Starch, 160 g;

Hydroxypropyl cellulose, 39 g; Polyvidone K30, q.s.; sodium carboxymethyl starch, 10.4 g; magnesium stearate, 1.3 g; for making 1000 tablets.

[0073] Preparation: Active ingredient, starch and Hydroxypropyl cellulose are placed in the hopper of a fluid bed granulator and warmed to 38-60 °C . Polyvidone K30 water solution is nebulized to granulate the mixture. The mixture is then dried at 55-60 °C for lOmin, mixed with sodium carboxymethyl starch and magnesium stearate to tabletting.

[0074] 3. Capsule. Formulation: Active ingredient, 50 g; lactose, 194.4 g; sodium carboxymethylstarch, 7.8 g; silion dioxide, 5.2 g; magnesium stearate, 2.6 g.

[0075] Preparation: Mix active ingredient, Lactose, sodium carboxymethylstarch and silion dioxide in the mixer for lh and then added magnesium stearate for another lOmin before filled in gelatin plastic shell.

[0076] Example 7. Toxicity, in vitro and in vivo effectiveness tests

[0077] Some of the above compounds are tested in vitro or in vivo for their anti-cancer or anti-tumor activities, as well as cellular toxicity tests using SRB and MTT methods. The in vitro anti-cancer effects on human cancer HT-29 and mouse lung cancer cell 3LL are summarized in Table 1, and the effects on treating transplanted human colon cancer HT-99 on nude mice are summarized in Table 2.

Table 1 In vitro anti-cancer cell activities IC₅₀ (μΜ)

Table 2: Effects on Human colon cancer HT-99 Transplanted Nude Mice

Notes: ip = intra-peritoneal injection

iv: intravenous injection;

GCT=gemcitabine

[0078] The data above show that the compounds of the present invention have antitumor effects, and can also enhance the anti-tumor effects of other compounds such as GCT, CPT-11, ADR.

[0079] Example 5 In vitro anticancer effects of XC608

[0080] Materials and Methods

[0081] Cell lines: human low differentiation stomach cancer BGC-823, human liver cancer QGY-7701, mouse lung cancer 3LL.

[0082] DMEM cell culture medium (GIBCO BRL), supplemented with 10% bovine fetal serum, L-glutamine, and antibiotics

[0083] 0.25% Trypsin solution (Invitrogen)

[0084] MTT Solution: made using phosphate buffer solution to 5 mg/ml.

[0085] Dissolution solution: SDS lOg, isobutanol 5 ml, and concentrated sulfuric acid

0.12 ml, added into 100 ml double distilled water.

[0086] Test compound and method of preparation The compound used for this test is

XC608, which is a whitish powder and has the following formula:

XC608

[0087] Control: XC302, another anticancer compound that is undergoing clinical trial.

[0088] Experimental Details: The cyto-toxicity of XC608 is measured using the MTT method. Specifically: (1) cell culture: (i) frozen cells are removed from liquid nitrogen, rapidly thawed in 37°C water bath; added to 6 ml DMEM culture medium in a 10 ml centrifuge tubes under sterile conditions, centrifuged at 1000 rpm for 5 min., with the supernatant removed. To the sediment is added 5-6 ml of DMEM medium, and re-suspended and cultured overnight at 37°C. (ii) The cells are centrifuged, washed twice with PBS, and treated with 3-4 drops of trypsin solution for about 3 minute at 37°C, and monitored under microscope to ensure that all cells are separated from the culture tube walls, and re-suspended in additional DMEM medium. The cells are separated into additional cultural vessels, and the above process repeated to ensure that the wall-adhered cells do not become overly dense and suspended cells remain in log-growth state. (2) Test compound preparation: XC608 and XC302 are dissolved in PBS and serial diluted to 1000, 100, 10, 1, 0.1 and 0.01 μg/ml. (3) The prepared test compound solution is added to the holes of a 96-hole culture plate, 10 μΐ/hole, with two holes per concentration point, and 10 μΐ PBS as control. (4) Prepare a suspension of log phase cell in a DMEM medium containing 10% new born bovine serum with a cell concentration of 2xl0⁵/ml. (5) add 90 μΐ cell suspension to each of the holes containing the test compound or control. (6) culture the cells at 37oC under 5% C02 for 48 hours. (7) Add 20 μΐ of 5 mg/ml MTT solution, and continue to culture for 3-4 hours. (8) Add 20 μΐ dissolution solution, and culture overnight. This ensures the formazan crystals are completely dissolved. (9) measure OD₅₇₀. (10) calculate relative cell survival rate by the formula: Relative cell survival rate = (treatment group OD - Background OD)/(PBS Control Group OD-Background OD) x 100%.

[0089] Results: The results are shown in Table 3 below:

Table 3. Inhibitive effects of X608 on cancer cell multiplication

[0090] Example 6. XC608 combined with Gemcitabine more effectively inhibits human ovarian cancer in nude mice

[0091] Objectives: Using the nude mice human ovarian cancer model (BALB/c-nu)to test the effect of XC608, XC608-C1, AZD7762 combined with Gemcitabine in cancer treatment.

[0092] Experimental Details [0093] 1. Test compound and method of preparation The compound used for this test is

XC608, shown above, and XC608-C1 is the chloride salt of XC608, 090208-1, also a whitish powder. AZD7762 is a yellow powder.

[0094] All of the above are dissolved in 5% glucose injectible solution and diluted to suitable concentrations. Control: Gemcitabine is obtained from Jiangsu Haosen Pharmaceuticals Co., Ltd., also dissolved to suitable concentration in saline solution.

[0095] Animals and Cancer cell lines: Test animals: 30 nude mice BALB/c-nu, female, bw 18-20 g, supplied by B&K Universal Group Limited , Shanghai, China. Cancer cell lines: the human ovarian cell line is from a commercial source, transplanted onto nude mice, which were maintained by Shanghai Pharmaceutical Industry Research Institute.

[0096] Test Methods: The tumors from the ovarian cancer carrying nude mice is isolated and cut into 1-mm pieces, which are then implanted subcutaneously to the test nude mice. After 10 days, the tumors grow to a size of about 0.1 cm . The test mice are randomly divided into 5 groups of six animals.

[0097] Treatment Groups: Gemcitabine 25 mg/kg+ XC608-F 50 mg/kg Gemcitabine

25 mg/kg+AZD7762 10 mg/kg, and control.

[0098] Starting from 11 days after tumor implant, the animals are administered the test compounds i.v. The animals are weighed every two days, and the tumor volume is measured. After 24 days from the beginning of test compound administration, the animals are killed and the tumors are isolated and weighed.

[0099] The tumor volume (TV) is measured and calculated.

[00100] Relative Tumor volume (RTV) is Vt/VO, wherein Vt is the tumor volume at the measurement time, while V0 is the tumor volume when drug administration was started.

[00101] A compound's antitumor activity is measured by the RTV growth rate, T/C ( ), which is calculated as: T/C = Treatment RTC/Control Group RTC x 100%.

[00102] If TC(%) is more than 60, then the treatment is considered to have no effect.

Only when the TC is less than 60, an the difference is statistically significant would the treatment be considered to be effective. [00103] Another measurement is rate of tumor inhibition, which is IR (%) = (control group tumor weight - treatment group tumor weight)/control group tumor weight x 100%.

[00104] Results: The results are summarized in the two tables below:

Table 4-1. Effects of XC608, and XC608-C1 (i.v.) combined with Gemcitabine in treating human ovarian cancer transplanted nude mice (body weight)

Table 4-2. Effects of XC608, and XC608-C1 (i.v.) combined with Gemcitabine in treating human ovarian cancer transplanted nude mice (tumor volume and weight)

Note: *p < 0.05; **p < 0.01; i.v. = intravenous injection

[00105] A graphical depiction of the above results in shown in Figure 1.

[00106] Example 7: Anti-cancer effects of XC608 and its mechanism

[00107] I. Summary

[00108] The experiments below examined the action mechanism and sensitizing effect of

XC608 on cytotoxic antitumor drugs, as well as its therapeutic effects when combined with 5- fluorouracil (5-Fu) and adriamycin (ADR) on nude mice with transplanted human colon cancer and breast cancer. [00109] Action Mechanism of XC608

[00110] XC608 clearly inhibits the activities of Chkl and Chk2, but has not apparent effect on c-met, HER2, or EGFR; it eliminates cdc2 phosphorylation induced by topoisomerase I, drives cells with DNA damages to continue to enter cell cycle; and clearly enhances the reaction of the cells to drugs that cause DNA damages. These results, taken together, demonstrate that XC608 is a specific Chkl inhibitor, and sensitizing other anticancer drugs by inhibiting the DNA repair process.

[00111] 2. Effects of XC608 on sensitizing cytotoxic anti-cancer drugs at the cellular level

[00112] The effects of XC608 and AZD7762 on a number of cytotoxic antitumor drugs are evaluated using cell cultures. The cytitoxic antitumor drugs include cisplatin (DDP), paclitaxel, pemetrexed, 5-fluorouracil (5-fu), vinorelbine, oxaliplatin, and gemcitabine are studied. The results show that XC608 has strong sensitizing effects on gemcitabine, DDP, 5-fu and pemetrexed, with that on gemcitabine being the highest, reaching more than 20 times.

[00113] 3. Animal tests showing the effects of XC608 on sensitizing cytotoxic anticancer drugs

[00114] Nude mice transplanted with human colon cancer HT-29 and SW-620 and breast cancer MDA-MB-231 are tested using XC608 alone and in combination with ADR and 5-fu. Results show that XC608 has significant sensitizing effects on the treatment effects of ADR and 5-fu on HT-29 and SW-620.

[00115] 4. Conclusion

[00116] XC608 is a specific Chkl inhibitor; it sensitizes the cancer cells to enhance the effects of other cytotoxic anticancer drugs by inhibiting DNA damage repair. Such sensitizing effects of XC608 is strongly correlated with the types of cytotoxic anticancer drugs as well as the types of cancer cell lines.

[00117] II. Action Mechanism of XC608

[00118] 1. Summary The effects of XC608 on the activity of protein kinases Chkl, Chk2 and c-met, and on cells entering cell cycle after drug-induced DNA damages and cellular reactions to DNA damages are studied. XC608 strongly inhibits the activities of Chk-1 and Chk-2, reduces topoisomerase I induced cdc-2 phosphorylation, and drives cells to continue to enter cell cycle. At the same time, XC608 clearly increased cellular susceptibility to DNA damages. These results show that XC608 inhibits DNA damage repair.

[00119] 2. Materials and Methods

[00120] XC608 and AZD-7762 are whitish powder, with a lot number of 090309-2 and

081107, respectively. They well dissolved in DMSO into 10 mM stock solution.

[00121] The K-LISA™ Chk- 1/Chk2 Activity Kit was supplied by Calibiochem (Cat. No.

CBA020). Chkl and Chk2 are from Upstate Biotechnology, Inc., and C-Met, HER2, EGFR are from Sigma. The Chk Peptide (KKKVSRSGLYRSPSMPENLNRPR) (SEQ ID NO: 1) is from Cell Signaling, Inc., (Beverly, MA, USA), and is stored at -70°C.

[00122] The cell line HT29 is purchased from ATCC, and cultured at 37°C with 5% C0₂ on Mycoy's 5A medium (GIBCO, Grand Island, NY, USA), which contains 10% bovine serum, L-glutamine, penicillin (100 IU/ml) and streptomycin (100 μg/ml).

[00123] Kinase activity measurements: Enzyme, sample, Chk peptide, 5 μιη ATP, and reaction buffer are added to the holes of biotinylated reaction plates. After the reaction is terminated with a stop buffer, the contents are discarded, replaced with 100 μΐ/hole of serine kinase antibody, to detect phosphorylation, and HRP-labeled goat-anti-rabbit IgG is added and incubated at room temperature for 1 hour. TMB reaction is conducted to determine substrate phosphorylation, with proper controls. OD450 is measured using VERSAmax (Sunnyvale, CA, USA). The rate of inhibition is calculated as the follows: Rate of Inhibition (I) = (reaction hole OD Value - OD value of Enzyme-free control)/(OD value of negative control - OD value of enzyme-free control) x 100%.

[00124] 4. Results

[00125] 4.1 Effects of XC608 on Protein Kinases

[00126] These results are shown in Table 5, which shows that XC608 has strong inhibitive effect on protein kinases.

Table 5 Effects of XC608 on Protein Kinase Activities

Drugs Inhibition (%)(Mean±SD)

Chkl Chk2 c-Met HER2 EGFR

XC608(l|iM) 85+11.1 90+13.2 15.9+ 10.2+1.3 15.9+5.3

AZD7762(100 nM) 80.6+11.3 99+0 / / / [00127] III. Effects of XC608 on Sensitizing Cancers Cells to Cytotoxic Anti-Cancer

Drugs

[00128] 1. Summary

[00129] The effects of XC608 and AZD7762 on a number of cytotoxic antitumor drugs are evaluated. The cytitoxic antitumor drugs include cisplatin (DDP), paclitaxel, pemetrexed, 5-fluorouracil (5-fu), vinorelbine, oxaliplatin, and gemcitabine are studied. The results show that XC608 has strong sensitizing effects on gemcitabine, DDP, 5-fu and pemetrexed, with that on gemcitabine being the highest, reaching more than 20 times.

[00130] 2. Objectives

[00131] The effects of XC608 in sensitizing the cells for a number of cytotoxic antitumor drugs are evaluated, and compared with that of a known sensitizer AZD7762.

[00132] 3. Materials and Methods

[00133] 3.1 Test Compounds: XC608 and AZD-7762 are whitish powder, with a lot number of 090309-2 and 081107, respectively.

[00134] 3.2 Cell Lines: HT-29 Cell line (from ATCC).

[00135] 3.3 Equipment/Devices: McCoy's 5a, from Gibco BRL; bovin fetal serum from

JRH; McCoy's 5a, from Gibco BRL; bovine fetal serum, from JRH Biosciences, Australia, SPECTRA MAX 190 microplate spectrophotometer, from Molecular Device ; SRB from Sigma.

[00136] 4. Test Protocol: The effects of the compounds on cancer cell multiplication was measured using Sulforhodamine B (SRB). Specifically, log phase cells are inoculated into the holes of 96-hole culture plates, allowed to grow using the wall-adhesion procedure at 37°C under 5% C0₂. The test compound at 9 different concentrations were added, each concentration in duplicate, with appropriate saline water and cell-less controls. The cells are cultured for a further 72 hours, and fixed with TCA, and SRB is added for staining, and washed, and air-dried. After adding the Tris solution, the OD value was measured at 510 nm using a Spectra Max 190. The rate of inhibition is calculated according to the formula below: Rate of Inhibition = (OD_controi -ODdrug)/OD_controi x 100%. [00137] The IC₅₀ is calculated based on the above ratios.

[00138] 5. Results The sensitizing effects of XCCS650(S) and AZD7762 are shown in

Table 6. Consistent with prior results, XC608 has strong sensitizing effects on gemcitabine, DDP, 5-fu and pemetrexed, with that on gemcitabine being the highest, reaching more than 20 times.

Table 6 Sensitizing Effects of XC608 on Cytocotix Antitumor Drugs

[00139] Conclusion: XC-608 is effective in sensitizing the cancer cells for cytotoxic anti-cancer drugs DDP, 5-Fu, oxaliplatin, and Gemcitabine.

[00140] Example 8: Anti-cancer effects of XC620 and its mechanism

[00141] A compound identical to XC608 except where in Formula I Y = O is synthesized and designated XC620. The action mechanism and sensitizing effect of XC620 on cytotoxic antitumor drugs, as well as its therapeutic effects when combined with 5-fluorouracil (5-Fu) and adriamycin (ADR) on nude mice with transplanted human colon cancer and breast cancer are also tested, following the protocols described above. The results show that XC620 also clearly inhibits the activities of Chkl and Chk2, and is a specific Chkl inhibitor, and sensitizing other anticancer drugs by inhibiting the DNA repair process.

[00142] The effects of XC620 on sensitizing cytotoxic anti-cancer drugs at the cellular level are comparable to XC608. It also has similar effect, though not as effective, on sensitizing tumor cell lines for cytitoxic antitumor drugs include cisplatin (DDP), paclitaxel, pemetrexed, 5-fluorouracil (5-fu), vinorelbine, oxaliplatin, and gemcitabine are studied.

[00143] Thus, XC620 is a also specific Chkl inhibitor; it sensitizes the cancer cells to enhance the effects of other cytotoxic anticancer drugs by inhibiting DNA damage repair.

[00144] Example 9: Anti-cancer effects of XC655 and its mechanism [00145] A compound identical to XC608 except where in Formula I Y = S is synthesized and designated XC620. The action mechanism and sensitizing effect of XC655 on cytotoxic antitumor drugs, as well as its therapeutic effects when combined with 5-fluorouracil (5-Fu) and adriamycin (ADR) on nude mice with transplanted human colon cancer and breast cancer are also tested, following the protocols described above. The results show that XC655 also clearly inhibits the activities of Chkl and Chk2, and is a specific Chkl inhibitor, and sensitizing other anticancer drugs by inhibiting the DNA repair process.

[00146] The effects of XC655 on sensitizing cytotoxic anti-cancer drugs at the cellular level are comparable to XC608. It also has similar effect, though not as effective, on sensitizing tumor cell lines for cytitoxic antitumor drugs include cisplatin (DDP), paclitaxel, pemetrexed, 5-fluorouracil (5-fu), vinorelbine, oxaliplatin, and gemcitabine are studied.

[00147] XC650 is therefore also specific Chkl inhibitor; it sensitizes the cancer cells to enhance the effects of other cytotoxic anticancer drugs by inhibiting DNA damage repair.

[00148] The foregoing description and examples have been set forth merely to illustrate the invention and are not intended to be limiting. Since modifications of the disclosed embodiments incorporating the spirit and substance of the invention may occur to persons skilled in the art, the invention should be construed broadly to include all variations falling within the scope of the appended claims and equivalents thereof. All references cited hereinabove and/or listed below are hereby expressly incorporated by reference.

Claims

What is claimed is:

1. A compound of formula I:

(I)

wherein

Y = NH, O, or S,

R¹ = selected from following groups:

wherein X = CH₂, NH, S, or O,

R⁸ = -H, -NH₂, -OH, -N(R⁴, R⁵), -C(R⁴R⁵)i_₇NR⁶R⁷, -C(R⁴R⁵)i_₇OR⁶, or -N(R⁴)NR⁵R⁶, wherein R⁴, R⁵, R⁶, R⁷ = H, alkyls (Ci-C ), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S, and N, aryls (either unsubstituted or substituted aromatics), or heteroaromatics (either unsubstituted or substituted heteroaromatics),

R², is selected from a group consisting of H, OH, NH₂, OR¹⁴, NR¹⁴R¹⁵, alkyl, aryl, heteroaryl, cycloalkyl, arylalkyl, heterocyclyl, heterocyclylalkyl, alkenyl, and alkynyl, wherein R , R = H, alkyls (C -C ), cycloalkyls (C₃-C₈) with or without nuclear heteroatoms such as O, S, N, aryls (either unsubstituted or substituted aromatics), or heteroaromatics (either unsubstituted or substituted heteroaromatics),

and

wherein R , R , and R are selected from following groups: H; heteroatom such as F, CI, Br, I; alkyliCi-Cs); cycloalkyl (C₃-C₈) without or with substitutions, wherein a substitution is selected from the group consisting of alkyls (Ci-C₈), cycloalkyls (C₃-C₈), aryls, heteroaryls; - OR¹⁹; -SR¹⁹; -NR¹⁹R²⁰; -S(0)R¹⁹; -S(0)₂R¹⁹; -S(0)₂NR¹⁹R²⁰; -C(0)NR¹⁹R²⁰; -N(R¹⁹)C(0)R²⁰; - N(R¹⁹)S(0)₂R²⁰; -N(R¹⁹)C(O)N(R²⁰R²¹); N(R¹⁹)C(0)OR²⁰; aryl with or without substitution, heteroaryl with or without substitution, aryalkyl with or without substitution, heterocyclyl with or without substitution, heteterocyclylalkyl with or without substitution; alkenyl with or without substitution, and alkynyl with or without substitution;

19 20 21

where R , R , and R are independently chosen from H, alkyl (Ci-C₈), cycloalkyls (C₃-C₈), aryl with or without substitution, alkylaryl with or without substitutions, heteroaryl with or without substitution, or R¹⁶, R¹⁷, and R²¹ can be part of a fused ring containing 0-3 heteroatoms selected from N, O, and S.

2. The compound of Claim 1, where Y=N.

3. The compound of Claim 1, where Y=S.

4. The compound of Claim 1, where Y=0.

5. The compound of Claim 1, selected from the group consisting of:

2-(4-fluorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(4-bromophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(4-fluorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-bromophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-fluorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-bromophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(S-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(R-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide 2-(4-chlorophenyl)-4-(3-piperidine-amino

acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide

2-(4-fluorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-bromophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-fluorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-bromophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-fluorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-bromophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-chlorophenyl)-4-(2-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(S-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(R-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(4-fluorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(4-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(4-bromophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(4-fluorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-chlorophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-bromophenyl)-4-(3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-fluorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-chlorophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-bromophenyl)-4-(3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2- (4-chlorophenyl)-4- 2- pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2- (4-chlorophenyl)-4- 4- pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2- (4-chlorophenyl)-4- 5- 3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2- (4-chlorophenyl)-4- R-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2- (4-chlorophenyl)-4- 3- pyridin-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-pyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-pyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(4-fluorophenyl)-4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-bromophenyl)-4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(4-fluorophenyl)-4- 3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-bromophenyl)-4- 3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(4-fluorophenyl)-4- 3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-chlorophenyl)-4- 3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-bromophenyl)-4- 3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (4-chlorophenyl)-4- 2-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(4-chlorophenyl)-4-(S-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(R-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(4-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(4-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-fluorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-bromophenyl)-4-(3-piperidineamino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-fluorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-bromophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-fluorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(3-bromophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(2-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(S-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(R-3-piperidine-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-piperidine-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothiapyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N-methyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyran-amino)-thieno[2,3-d]pyridazine-7- N,N-dimethyl- carboxylic acid amide

2-(3-fluorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-bromophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-fluorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide 2-(3-bromophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-fluorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-bromophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(2-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(S-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(R-3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydropyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrothieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N-methyl- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-tetrahydrofuran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl- carboxylic acid amide

2-(3-fluorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(3-chlorophenyl)-4-(3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(3-bromophenyl)-4- 3-pyridin-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2- (3 -fluorophenyl) -4- 3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-bromophenyl)-4- 3-a-pyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2- (3 -fluorophenyl) -4- 3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 3-a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-bromophenyl)-4- 3- a-thiapyran-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 2- pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 4- pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 5- 3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- R-3-pyridin-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4- 3- pyridin-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-pyridin-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-thiapyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-pyran-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-pyran-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2- (3 -fluorophenyl) -4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-chlorophenyl)-4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-bromophenyl)-4- 3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide 2-(3-fluorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-bromophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-fluorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-bromophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-carboxylic acid amide

2-(3-chlorophenyl)-4-(2-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(S-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(R-3-pyrrol-amino)-thieno[2,3-d]pyridazine-7- carboxylic acid amide

2-(3-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-pyrrol-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-thieno-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide

2-(3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N-methyl-carboxylic acid amide, and

2-(3-chlorophenyl)-4-(3-furan-amino)-thieno[2,3-d]pyridazine-7-N,N-dimethyl-carboxylic acid amide.

6. A pharmaceutical composition comprising a therapeutically effective amount of the compound of claim 1 and a pharmaceutically acceptable excipient.

7. A method for treating cancer, comprising administering to a patient in need thereof a therapeutically effective amount of the pharmaceutical composition of claim 5.