WO2012061331A3 - Filamentous fungi and methods for producing isoprenoids - Google Patents

Filamentous fungi and methods for producing isoprenoids Download PDF

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Publication number
WO2012061331A3
WO2012061331A3 PCT/US2011/058700 US2011058700W WO2012061331A3 WO 2012061331 A3 WO2012061331 A3 WO 2012061331A3 US 2011058700 W US2011058700 W US 2011058700W WO 2012061331 A3 WO2012061331 A3 WO 2012061331A3
Authority
WO
WIPO (PCT)
Prior art keywords
filamentous fungi
methods
isoprenoid
producing isoprenoids
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/058700
Other languages
French (fr)
Other versions
WO2012061331A2 (en
Inventor
Thomas M. Hohn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Priority to BR112013008045A priority Critical patent/BR112013008045A2/en
Priority to US13/819,198 priority patent/US20130224817A1/en
Priority to EP11838644.0A priority patent/EP2635672A2/en
Priority to CN2011800636420A priority patent/CN103282484A/en
Publication of WO2012061331A2 publication Critical patent/WO2012061331A2/en
Publication of WO2012061331A3 publication Critical patent/WO2012061331A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungi isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03006—Trichodiene synthase (4.2.3.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03047—Beta-farnesene synthase (4.2.3.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/77—Fusarium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to the production of a isoprenoid products from a lignocellulosic feedstock. Specifically at least triple mutant of filamentous fungi having the isoprenoid pathway results in production of isoprenoid products in commercial quantities. One embodiment of the invention relates to producing the isoprenoid products at the site of the lignocellulosic feedstock to reduce costs of shipping the feedstock.
PCT/US2011/058700 2010-11-01 2011-11-01 Filamentous fungi and methods for producing isoprenoids Ceased WO2012061331A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR112013008045A BR112013008045A2 (en) 2010-11-01 2011-11-01 mutant isoprenoid-producing filamentous fungus, and method of producing isoprenoid
US13/819,198 US20130224817A1 (en) 2010-11-01 2011-11-01 Filamentous Fungi and Methods for Producing Isoprenoids
EP11838644.0A EP2635672A2 (en) 2010-11-01 2011-11-01 Filamentous fungi and methods for producing isoprenoids
CN2011800636420A CN103282484A (en) 2010-11-01 2011-11-01 Filamentous fungi and methods for producing isoprenoids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40867910P 2010-11-01 2010-11-01
US61/408,679 2010-11-01

Publications (2)

Publication Number Publication Date
WO2012061331A2 WO2012061331A2 (en) 2012-05-10
WO2012061331A3 true WO2012061331A3 (en) 2012-07-26

Family

ID=46025052

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/058700 Ceased WO2012061331A2 (en) 2010-11-01 2011-11-01 Filamentous fungi and methods for producing isoprenoids

Country Status (5)

Country Link
US (1) US20130224817A1 (en)
EP (1) EP2635672A2 (en)
CN (1) CN103282484A (en)
BR (1) BR112013008045A2 (en)
WO (1) WO2012061331A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2847331A4 (en) * 2012-05-11 2015-11-25 Teknologian Tutkimuskeskus Vtt Oy PROCESS FOR PRODUCING TERPENA
CN106987578B (en) * 2017-04-10 2020-01-07 武汉大学 A kind of terpenoid synthase for producing koraiol and its application
CN107954839B (en) * 2017-12-04 2020-05-05 云南大学 Anti-inflammatory active compound peniroquesine A and preparation method and application thereof
CN109988219B (en) * 2019-04-22 2020-06-05 云南大学 A kind of sesquiterpene cyclohexenone compound and its preparation method and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696282B2 (en) * 1999-07-23 2004-02-24 The United States Of America As Represented By The Secretary Of Agriculture Fusarium sporotrichioides strains for production of lycopene
WO2011017549A2 (en) * 2009-08-05 2011-02-10 Dorsan Biofuels, Inc. Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696282B2 (en) * 1999-07-23 2004-02-24 The United States Of America As Represented By The Secretary Of Agriculture Fusarium sporotrichioides strains for production of lycopene
WO2011017549A2 (en) * 2009-08-05 2011-02-10 Dorsan Biofuels, Inc. Filamentous fungi and methods for producing trichodiene from lignocellulosic feedstocks

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANDREW W. PEPLOW ET AL.: "Identification of new genes positively regulated by Tri1O and a regulatory network for trichothecene Mycotoxin production.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 69, no. 5, May 2003 (2003-05-01), pages 2731 - 2736, XP007921338 *
JAYANAND BODDU ET AL.: "Transcriptome analysis of trichothecene-induced gene expression in barley", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 20, no. 11, November 2007 (2007-11-01), pages 1364 - 1375, XP055090257 *
KYE-YONG SEONG ET AL.: "Global gene regulation by Fusarium transcription factors Tri6 and TrilO reveals adaptations for toxin biosynthesis", MOLECULAR MICROBIOLOGY, vol. 72, no. 2, 17 March 2009 (2009-03-17), pages 354 - 367, XP055046150 *
ROBERT H. PROCTOR ET AL.: "Tri6 encodes an unusual zinc finger protein involved in regulation of trichothecene biosynthesis in Fusarium sporotrichioides", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 61, no. 5, May 1995 (1995-05-01), pages 1923 - 1930, XP002125090 *

Also Published As

Publication number Publication date
WO2012061331A2 (en) 2012-05-10
US20130224817A1 (en) 2013-08-29
BR112013008045A2 (en) 2016-06-14
CN103282484A (en) 2013-09-04
EP2635672A2 (en) 2013-09-11

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