WO2012068248A3 - Methods for preserving target cells - Google Patents

Methods for preserving target cells Download PDF

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Publication number
WO2012068248A3
WO2012068248A3 PCT/US2011/060990 US2011060990W WO2012068248A3 WO 2012068248 A3 WO2012068248 A3 WO 2012068248A3 US 2011060990 W US2011060990 W US 2011060990W WO 2012068248 A3 WO2012068248 A3 WO 2012068248A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
target cells
preserving target
preserving
degradable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/060990
Other languages
French (fr)
Other versions
WO2012068248A2 (en
Inventor
Michael Coleman
Jody Vykoukal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
InGeneron Inc
Original Assignee
InGeneron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by InGeneron Inc filed Critical InGeneron Inc
Priority to EP11840811.1A priority Critical patent/EP2640827B1/en
Publication of WO2012068248A2 publication Critical patent/WO2012068248A2/en
Publication of WO2012068248A3 publication Critical patent/WO2012068248A3/en
Priority to US13/896,234 priority patent/US20130260459A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/415—Assays involving biological materials from specific organisms or of a specific nature from plants
    • G01N2333/42—Lectins, e.g. concanavalin, phytohaemagglutinin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70582—CD71
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70589—CD45
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Oncology (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Methods for obtaining and preserving target cells using degradable three dimensional matrices are described.
PCT/US2011/060990 2010-11-16 2011-11-16 Methods for preserving target cells Ceased WO2012068248A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP11840811.1A EP2640827B1 (en) 2010-11-16 2011-11-16 Methods for preserving target cells
US13/896,234 US20130260459A1 (en) 2010-11-16 2013-05-16 Methods for Preserving Target Cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41433510P 2010-11-16 2010-11-16
US61/414,335 2010-11-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/896,234 Continuation US20130260459A1 (en) 2010-11-16 2013-05-16 Methods for Preserving Target Cells

Publications (2)

Publication Number Publication Date
WO2012068248A2 WO2012068248A2 (en) 2012-05-24
WO2012068248A3 true WO2012068248A3 (en) 2012-06-21

Family

ID=46084617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/060990 Ceased WO2012068248A2 (en) 2010-11-16 2011-11-16 Methods for preserving target cells

Country Status (3)

Country Link
US (1) US20130260459A1 (en)
EP (1) EP2640827B1 (en)
WO (1) WO2012068248A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104508122A (en) * 2012-06-20 2015-04-08 赛璐拉有限公司 Materials and methods for processing cell populations
IT201600111352A1 (en) * 2016-11-04 2018-05-04 Univ Campus Bio Medico Di Roma POROUS MATERIAL FOR THE INCLUSION OF CYTOLOGICAL PREPARATIONS, PROCEDURE FOR OBTAINING THE SAME AND ITS USE
AU2019265019B2 (en) 2018-05-11 2025-11-06 Beam Therapeutics Inc. Methods of substituting pathogenic amino acids using programmable base editor systems
US12426594B2 (en) 2020-09-24 2025-09-30 Everest Medical Innovation GmbH Cryoprotective compositions and methods for protection of a surgical site during cryosurgery
US12453805B2 (en) 2020-09-24 2025-10-28 Everest Medical Innovation GmbH Cryoprotective compositions, surgical kits, and methods for protection of a surgical site during cryosurgery

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007129828A1 (en) * 2006-05-04 2007-11-15 Seoul National University Industry Foundation Preparation method of porous hyaluronic acid sponge for cell delivery system
US20100239671A1 (en) * 2007-11-06 2010-09-23 Edelman Elazer R Tissue-Engineered Endothelial and Epithelial Implants Differentially and Synergistically Regulate Tissue Repair

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE456164B (en) * 1980-08-20 1988-09-12 Kjell Nilsson PROCEDURE FOR IMMOBILIZATION, CULTURE AND SUBSEQUENT RELEASE OF ANIMAL CELLS AND MICROBELLERS OF GELATIN WITH ABSORBED ANIMAL CELLS
DE4341005C2 (en) * 1993-11-29 1996-10-24 Ljuba Dr Rer Nat Mavrina Method for the quantitative determination of the residual leukocytes in a sample of a blood product
US5885829A (en) 1996-05-28 1999-03-23 The Regents Of The University Of Michigan Engineering oral tissues
US6184011B1 (en) * 1999-03-22 2001-02-06 Cbd Technologies, Ltd Method of releasing solid matrix affinity adsorbed particulates
KR100930139B1 (en) * 2001-12-07 2009-12-07 사이토리 테라퓨틱스, 인크. Systems and methods for treating patients with treated lipoasperate cells
US20050100877A1 (en) * 2003-09-02 2005-05-12 University Of North Carolina At Chapel Hill Biodegradable polymer-ligand conjugates and their uses in isolation of cellular subpolulations and in cryopreservation, culture and transplantation of cells
US20060063141A1 (en) 2004-09-17 2006-03-23 Mcgann Locksley E Method of cryopreserving cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007129828A1 (en) * 2006-05-04 2007-11-15 Seoul National University Industry Foundation Preparation method of porous hyaluronic acid sponge for cell delivery system
US20100239671A1 (en) * 2007-11-06 2010-09-23 Edelman Elazer R Tissue-Engineered Endothelial and Epithelial Implants Differentially and Synergistically Regulate Tissue Repair

Also Published As

Publication number Publication date
US20130260459A1 (en) 2013-10-03
EP2640827B1 (en) 2023-07-19
EP2640827A2 (en) 2013-09-25
WO2012068248A2 (en) 2012-05-24
EP2640827A4 (en) 2017-07-19

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