WO2012129036A3 - Promoters for high level recombinant expression in fungal host cells - Google Patents

Promoters for high level recombinant expression in fungal host cells Download PDF

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Publication number
WO2012129036A3
WO2012129036A3 PCT/US2012/029146 US2012029146W WO2012129036A3 WO 2012129036 A3 WO2012129036 A3 WO 2012129036A3 US 2012029146 W US2012029146 W US 2012029146W WO 2012129036 A3 WO2012129036 A3 WO 2012129036A3
Authority
WO
WIPO (PCT)
Prior art keywords
promoters
host cells
recombinant expression
fungal host
high level
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/029146
Other languages
French (fr)
Other versions
WO2012129036A2 (en
Inventor
Robert Davidson
Bianka Prinz
I-Ming Wang
Stephen Hamilton
Ming-Tang Chen
Brian MICKUS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Organon Pharma UK Ltd
Merck Sharp and Dohme LLC
Original Assignee
Merck Sharp and Dohme Ltd
Merck Sharp and Dohme LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp and Dohme Ltd, Merck Sharp and Dohme LLC filed Critical Merck Sharp and Dohme Ltd
Priority to EP12760797.6A priority Critical patent/EP2689023A2/en
Priority to US14/005,340 priority patent/US20140011236A1/en
Publication of WO2012129036A2 publication Critical patent/WO2012129036A2/en
Anticipated expiration legal-status Critical
Publication of WO2012129036A3 publication Critical patent/WO2012129036A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides, in part, promoters for recombinant expression of polypeptides in host cells such as Pichia pastoris as well as methods of use thereof. In one embodiment, an isolated hybrid polynucleotide comprises a promoter selected from a group of Pichia pastoris promoters. In another embodiment, a method for making a polypeptide by introducing an isolated hybrid polynucleotide into an isolated fungal host cell while utilizes a Pichia pastoris promoter.
PCT/US2012/029146 2011-03-22 2012-03-15 Promoters for high level recombinant expression in fungal host cells Ceased WO2012129036A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP12760797.6A EP2689023A2 (en) 2011-03-22 2012-03-15 Promoters for high level recombinant expression in fungal host cells
US14/005,340 US20140011236A1 (en) 2011-03-22 2012-03-15 Promoters for high level recombinant expression in fungal host cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161466220P 2011-03-22 2011-03-22
US61/466,220 2011-03-22
US201161473426P 2011-04-08 2011-04-08
US61/473,426 2011-04-08

Publications (2)

Publication Number Publication Date
WO2012129036A2 WO2012129036A2 (en) 2012-09-27
WO2012129036A3 true WO2012129036A3 (en) 2014-01-03

Family

ID=46879969

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/029146 Ceased WO2012129036A2 (en) 2011-03-22 2012-03-15 Promoters for high level recombinant expression in fungal host cells

Country Status (3)

Country Link
US (1) US20140011236A1 (en)
EP (1) EP2689023A2 (en)
WO (1) WO2012129036A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2669375B1 (en) * 2011-01-27 2018-10-17 Kaneka Corporation Yeast of the genus pichia modified to express high levels of a mpp1 homolog and process for producing protein
US9255154B2 (en) 2012-05-08 2016-02-09 Alderbio Holdings, Llc Anti-PCSK9 antibodies and use thereof
US20150299648A1 (en) * 2012-11-29 2015-10-22 Merck Sharp & Dohme Corp Improved cultivation media and process for improved protein production by pichia strains
BR112015021753B1 (en) * 2013-03-08 2023-02-23 Keck Graduate Institute Of Applied Life Sciences NUCLEIC ACIDS ISOLATED FROM PICHIA PASTORIS, EXPRESSION VECTOR, YEAST HOST CELLS AND DNA CONSTRUCT, AS WELL AS METHOD FOR PRODUCING A PROTEIN
HUE042897T2 (en) * 2013-03-08 2019-07-29 Biogrammatics Inc Yeast promoters for protein expression
WO2016057954A2 (en) 2014-10-10 2016-04-14 Board Of Regents Of The University Of Texas System Exogenous terminators for controlling fungal gene expression
WO2016089516A2 (en) * 2014-10-31 2016-06-09 Board Of Regents, The University Of Texas System Short exogenous promoter for high level expression in fungi
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
CN115793401A (en) * 2017-03-15 2023-03-14 Asml荷兰有限公司 Apparatus for delivering gas and irradiation source for generating high harmonic radiation
CN107841556B (en) * 2017-12-19 2020-12-01 武汉大学 A kit and application for screening drug developmental toxicity based on C/EBPα and IGF1R genes
US11866714B2 (en) 2018-06-07 2024-01-09 Basf Se Promoter for yeast
KR20220034848A (en) 2019-07-11 2022-03-18 클라라 푸드즈 컴퍼니 Protein composition and edible products thereof
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
CN115960736B (en) * 2023-03-01 2024-05-28 石河子大学 A kind of engineering yeast of saccharomyces cerevisiae producing vanillylamine and capsaicin and its construction method and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258559B1 (en) * 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
US20100196913A1 (en) * 2005-02-23 2010-08-05 Technische Universitat Graz Mutant AOX1 Promoters

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6258559B1 (en) * 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
US20100196913A1 (en) * 2005-02-23 2010-08-05 Technische Universitat Graz Mutant AOX1 Promoters

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RUTH ET AL.: "Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris.", SYST. SYTH. BIOL., vol. 4, 29 May 2010 (2010-05-29), pages 181 - 191 *

Also Published As

Publication number Publication date
EP2689023A2 (en) 2014-01-29
WO2012129036A2 (en) 2012-09-27
US20140011236A1 (en) 2014-01-09

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