WO2013074492A1

WO2013074492A1 - Methods of treating cancer

Info

Publication number: WO2013074492A1
Application number: PCT/US2012/064772
Authority: WO
Inventors: Thomas Harding; Servando Palencia; Li Long; Kevin Hestir
Original assignee: Five Prime Therapeutics Inc
Current assignee: Five Prime Therapeutics Inc
Priority date: 2011-11-14
Filing date: 2012-11-13
Publication date: 2013-05-23
Anticipated expiration: 2014-05-14
Also published as: TW201326201A; US10016484B2; HK1201462A1; EP2780033B1; US20140341900A1; EP2780033A4; EP2780033A1; US20190000920A1; US20130136740A1; CA2855818A1; AU2012318247B2; AU2012318247A1; CN104168915A; JP2015505818A; JP2017206506A; JP6578318B2; CN107823630A; HK1202240A1; US10537611B2

Abstract

Methods of treating cancers comprising FGFR1 gene amplification are provided. In some embodiments, the methods comprise administering a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) and/or an FGFR1 ECD fusion molecule. In some embodiments, the methods comprise administering a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) and/or an FGFR1 ECD fusion molecule in combination with at least one additional therapeutic agent.

Description

METHODS OF TREATING CANCER

[0001] This application claims the benefit of U.S. Provisional Application No.

61/559,259, filed November 14, 2011 ; and U.S. Provisional Application No. 61/616,761, filed March 28, 2012, which are incorporated herein by reference in their entireties for any purpose.

BACKGROUND

[0002] Soluble forms of Fibroblast Growth Factor Receptor 1 (FGFRl) have been shown to inhibit tumor cell growth in vitro and in vivo. See, e.g., U.S. Patent No. 7,678,890. The efficacy of anti-cancer therapies is, in some instances, dependent on the genetic makeup of the cancer being targeted.

SUMMARY OF THE INVENTION

[0003] The inventors have demonstrated that certain cancers that comprise FGFRl gene amplification are, in some embodiments, more responsive to therapies involving

administration of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or FGFRl ECD fusion molecule, than cancers that do not comprise an FGFRl gene amplification. In some embodiments, cancers that have FGFRl overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have FGFRl overexpression. In some embodiments, FGFRl is FGFRlIIIc. In some embodiments, cancers that have fibroblast growth factor receptor 3 isoform IIIc (FGFR3IIIc) overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have FGFR3IIIc overexpression. In some embodiments, cancers that have fibroblast growth factor 2 (FGF2) overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have FGF2 overexpression. In some embodiments, cancers that have dickkopf-related protein 3 (DKK3) overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have DKK3 overexpression. In some embodiments, cancers that have ETS translocation variant 4 (ETV4) overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have ETV4 overexpression. In some embodiments, cancers that have FGF 18 overexpression are more responsive to therapies involving administration of FGFRl ECD or FGFRl ECD fusion molecules, than cancers that do not have FGF 18 overexpression. [0004] In some embodiments, methods of treating a cancer having an FGFRl gene amplification, wherein an FGFRl gene amplification is indicative of therapeutic

responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have an FGFRl gene amplification, and wherein an FGFRl gene amplification in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

[0005] In some embodiments, methods of treating a lung cancer having an FGFRl gene amplification, wherein an FGFRl gene amplification is indicative of therapeutic

responsiveness by the lung cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating lung cancer in a subject comprise administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the lung cancer have been determined to have an FGFRl gene amplification, and wherein an FGFRl gene amplification in a cancer is indicative of therapeutic responsiveness of the lung cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the lung cancer is small cell lung cancer. In some embodiments, the lung cancer is non-small cell lung cancer.

[0006] In some embodiments, at least a portion of the cells of the cancer comprise at least three, at least four, at least five, at least six, at least eight, or at least ten copies of the FGFRl gene. In some embodiments, at least a portion of the cells of the cancer have a ratio of FGFRl gene to chromosome 8 centromere of at least 1.5, at least 2, at least 2.5, at least 3, at least 3.5, or at least 4.

[0007] In some embodiments, including any of the foregoing embodiments, the cancer may overexpress at least one, at least two, at least three, at least four, or at least five markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4. In some embodiments, the cancer may overexpress at least one, at least two, at least three, at least four, or five markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, and FGF18. In some

embodiments, the cancer may overexpress ETV4. In some embodiments, including any of the foregoing embodiments, the cancer may overexpress Gene 1 and Gene 2 from any line in Table 10 below, or any comination thereof. In some embodiments, FGFRl is FGFRlIIIc. In some embodiments, including any of the foregoing embodiments, the FGFRl gene may be amplified.

[0008] In some embodiments, methods of treating a cancer that overexpress at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 are provided. In some embodiments, overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule. In some embodiments, a method comprises administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to a subject with cancer that overexpress at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to overexpress at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4, and wherein overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the cancer also has an FGFRl gene amplification. In some embodiments, at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least three, at least four, at least five, at least six, at least seven, or at least eight copies of the FGFRl gene. In some embodiments, the overexpression is mRNA overexpression. In some embodiments, mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the overexpression is protein overexpression. In some embodiments, protein overexpression is determined by immunohistochemistry. In some embodiments, FGFRl is FGFRlIIIc. [0009] In some embodiments, methods of treating a cancer having FGFRl overexpression, wherein FGFRl overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have FGFRl overexpression, and wherein FGFRl overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the cancer does not have an FGFRl gene amplification. In some embodiments, the FGFRl overexpression is mRNA overexpression. In some embodiments, FGFRl mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the FGFRl overexpression is protein overexpression. In some embodiments, FGFRl protein overexpression is determined by immunohistochemistry. In some embodiments, FGFRl is FGFRl IIIc.

[0010] In some embodiments, methods of treating a cancer having FGFR3IIIc overexpression, wherein FGFR3IIIc overexpression is indicative of therapeutic

responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have

FGFR3IIIc overexpression, and wherein FGFR3IIIc overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the cancer does not have an FGFRl gene amplification. In some embodiments, the FGFR3IIIc overexpression is mRNA overexpression. In some embodiments, FGFR3IIIc mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the FGFR3IIIc overexpression is protein overexpression. In some embodiments, FGFR3IIIc protein overexpression is determined by immunohistochemistry. In some embodiments, the cancer having FGFR3IIIc overexpression is selected from bladder cancer, renal cell carcinoma, head-and-neck squamous carcinoma, and colorectal cancer. [0011] In some embodiments, methods of treating a cancer having FGF2 overexpression, wherein FGF2 overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have FGF2 overexpression, and wherein FGF2 overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the cancer does not have an FGFRl gene amplification. In some embodiments, the FGF2 overexpression is mRNA overexpression. In some embodiments, FGF2 mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the FGF2 overexpression is protein overexpression. In some embodiments, FGF2 protein overexpression is determined by immunohistochemistry. In some embodiments, the cancer having FGF2 overexpression is selected from glioblastoma, renal cell carcinoma, and hepatocellular carcinoma.

[0012] In some embodiments, methods of treating a cancer having DKK3 overexpression, wherein DKK3 overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have DKK3 overexpression, and wherein DKK3 overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the DKK3 overexpression is mRNA overexpression. In some embodiments, DKK3 mRNA

overexpression is determined by quantitative RT-PCR. In some embodiments, the DKK3 overexpression is protein overexpression. In some embodiments, DKK3 protein

overexpression is determined by immunohistochemistry. In some embodiments, the cancer having DKK3 overexpression is selected from pancreatic cancer, prostate cancer, renal cell carcinoma, lung adenocarcinoma, hepatocellular cancer, and colorectal cancer. [0013] In some embodiments, methods of treating a cancer having FGF18 overexpression, wherein FGF18 overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to

administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have FGF18 overexpression, and wherein FGF 18 overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the FGF18 overexpression is mRNA overexpression. In some embodiments, FGF18 mRNA

overexpression is determined by quantitative RT-PCR. In some embodiments, the FGF18 overexpression is protein overexpression. In some embodiments, FGF18 protein

overexpression is determined by immunohistochemistry.

[0014] In some embodiments, methods of treating a cancer having ETV4 overexpression, wherein ETV4 overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, methods of treating cancer in a subject comprise administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject, wherein, prior to

administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have ETV4 overexpression, and wherein ETV4 overexpression in a cancer is indicative of therapeutic responsiveness of the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the ETV4

overexpression is mRNA overexpression. In some embodiments, ETV4 mRNA

overexpression is determined by quantitative RT-PCR. In some embodiments, the ETV4 overexpression is protein overexpression. In some embodiments, ETV4 protein

overexpression is determined by immunohistochemistry.

[0015] In some embodiments, methods of treating a lung cancer having FGFRl overexpression, wherein FGFRl overexpression is indicative of therapeutic responsiveness by the lung cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFR1 ECD or an FGFR1 ECD fusion molecule to the subject. In some embodiments, methods of treating lung cancer in a subject comprise administering a therapeutically effective amount of an FGFR1 ECD or an FGFR1 ECD fusion molecule to the subject, wherein, prior to administration of the FGFR1 ECD or FGFR1 ECD fusion molecule, at least a portion of the cells of the lung cancer have been determined to have FGFR1 overexpression, and wherein FGFR1 overexpression in a cancer is indicative of therapeutic responsiveness of the lung cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule. In some embodiments, the cancer does not have an FGFR1 gene amplification. In some embodiments, the lung cancer is small cell lung cancer. In some embodiments, the lung cancer is non-small cell lung cancer. In some embodiments, FGFR1 is FGFRlIIIc.

[0016] In some embodiments, methods of treating a lung cancer having FGF2

overexpression, wherein FGF2 overexpression is indicative of therapeutic responsiveness by the lung cancer to a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) or an FGFR1 ECD fusion molecule, comprise administering a therapeutically effective amount of an FGFR1 ECD or an FGFR1 ECD fusion molecule to the subject. In some embodiments, methods of treating lung cancer in a subject comprise administering a therapeutically effective amount of an FGFR1 ECD or an FGFR1 ECD fusion molecule to the subject, wherein, prior to administration of the FGFR1 ECD or FGFR1 ECD fusion molecule, at least a portion of the cells of the lung cancer have been determined to have FGF2 overexpression, and wherein FGF2 overexpression in a cancer is indicative of therapeutic responsiveness of the lung cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule. In some embodiments, the cancer does not have an FGFR1 gene amplification. In some embodiments, the lung cancer is small cell lung cancer. In some embodiments, the lung cancer is non-small cell lung cancer. In some embodiments, the lung cancer does not have an FGFR1 gene amplification.

[0017] In some embodiments, a method of treating a cancer having an FGFR1 gene amplification comprises administering an FGFR1 ECD or FGFR1 ECD fusion molecule and at least one additional therapeutic agent. In some embodiments, a method of treating a cancer that overexpresses at least one, at least two, at least three, or at least four markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 comprises administering an FGFR1 ECD or FGFR1 ECD fusion molecule and at least one additional therapeutic agent. In some embodiments, at least one additional therapeutic agent is selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, etoposide, topotecan, sorafenib, a VEGF antagonist, a VEGF trap, an anti-VEGF antibody, and bevacizumab. In some embodiments, the at least one additional therapeutic agent is docetaxel. In some embodiments, the cancer is non-small cell lung cancer. In some embodiments, the FGFRl is FGFRlIIIc.

[0018] In some embodiments, a method of treating a cancer having an FGFRl gene amplification comprises administering an FGFRl ECD or FGFRl ECD fusion molecule and at least two additional therapeutic agents. In some embodiments, a method of treating a cancer that overexpresses at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF 18, and ETV4 comprises

administering an FGFRl ECD or FGFRl ECD fusion molecule and at least two additional therapeutic agents. In some embodiments, at least two additional therapeutic agents are selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, etoposide, topotecan, sorafenib, a VEGF antagonist, a VEGF trap, an anti-VEGF antibody, and bevacizumab. In some embodiments, the two additional therapeutic agents are paclitaxel and carboplatin. In some embodiments, the two additional therapeutic agents are doxorubicin and paclitaxel. In some embodiments, the two additional therapeutic agents are cisplatin and etoposide. In some embodiments, the two additional therapeutic agents are oxaliplatin and 5-FU. In some embodiments, the two additional therapeutic agents are 5-FU and leucovorin. In some embodiments, the two additional therapeutic agents are 5-FU and bevacizumab. In some embodiments, the two additional therapeutic agents are paclitaxel and bevacizumab. In some embodiments, the cancer is non-small cell lung cancer.

[0019] In some embodiments, a method of treating a cancer having an FGFRl gene amplification comprises administering an FGFRl ECD or FGFRl ECD fusion molecule and at least three additional therapeutic agents. In some embodiments, a method of treating a cancer that overexpresses at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF 18, and ETV4 comprises

administering an FGFRl ECD or FGFRl ECD fusion molecule and at least three additional therapeutic agents. In some embodiments, at least three additional therapeutic agents are selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, etoposide, topotecan, sorafenib, a VEGF antagonist, a VEGF trap, an anti-VEGF antibody, and bevacizumab. In some embodiments, the three additional therapeutic agents are oxaliplatin, 5-FU and leucovorin. In some embodiments, the three additional therapeutic agents are bevacizumab, 5-FU and leucovorin. [0020] In some embodiments, methods of treating a cancer having an FGFRl gene amplification and/or that overexpresses at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 comprise administering an FGFRl ECD. In some such embodiments, the FGFRl ECD comprises an amino acid sequence selected from SEQ ID NOs: 1 to 4. In some embodiments, methods of treating a cancer having an FGFRl gene amplification and/or FGFRl overexpression and/or FGF2 overexpression and/or DKK3 overexpression and/or FGF 18 overexpression and/or ETV4 overexpression comprise administering an FGFRl ECD fusion molecule, wherein the FGFRl ECD fusion molecule comprises an FGFRl ECD and at least one fusion partner. In some embodiments, at least one fusion partner is selected from an Fc, albumin, and polyethylene glycol. In some embodiments, at least one fusion partner is an Fc. In some embodiments, the Fc comprises an amino acid sequence selected from SEQ ID NOs: 8 to 10. In some embodiments, the FGFRl ECD fusion molecule comprises a sequence selected from SEQ ID NO: 5 and SEQ ID NO: 6. In some embodiments, the at least one fusion partner is an Fc and polyethylene glycol. In some embodiments, the at least one fusion partners is polyethylene glycol. In some embodiments, the fusion molecule comprises a linker between the FGFRl ECD and one or more fusion partners. In some embodiments, the FGFRl ECD fusion molecule is FGFRl ECD.339-Fc.

[0021] In some embodiments, an FGFRl ECD or FGFRl ECD fusion molecule is glycosylated and/or sialylated. In some embodiments, an FGFRl ECD or the polypeptide portion of the FGFRl ECD fusion molecule is expressed in Chinese hamster ovary (CHO) cells. In some embodiments, an FGFRl ECD comprises an amino acid sequence selected from SEQ ID NO: 1 and SEQ ID NO: 3.

[0022] In some embodiments, the FGFRl ECD or FGFRl ECD fusion molecule is an amount in the range of about 0.5 mg/kg body weight to about 30 mg/kg body weight, such as an amount in the range of about 8 to about 16 mg/kg body weight. In some embodiments, the therapeutically effective amount of the FGFRl ECD or FGFRl ECD fusion molecule is a dose of about 8 mg/kg body weight. In some embodiments, the therapeutically effective amount of the FGFRl ECD or FGFRl ECD fusion molecule is a dose of about 16 mg/kg body weight. In some embodiments, the therapeutically effective amount of the FGFRl ECD or FGFRl ECD fusion molecule is a dose of about 20 mg/kg body weight. In some embodiments, dosages may be administered twice a week, weekly, every other week, at a frequency between weekly and every other week, every three weeks, every four weeks, or every month. [0023] In certain embodiments, the cancer is prostate cancer, breast cancer, colorectal cancer, lung cancer, brain cancer, ovarian cancer, endometrial cancer, esophageal cancer, head and neck cancer, laryngeal cancer, liver cancer, renal cancer, glioblastoma, or pancreatic cancer. In certain embodiments, the cancer is breast cancer, esophageal cancer, renal cancer, head and neck cancer, or lung cancer. In certain embodiments, the cancer is lung cancer. In some embodiments, the lung cancer is non-small cell lung cancer. In some embodiments, the lung cancer is small cell lung cancer. In some embodiments, the lung cancer is squamous cell carcinoma. In some embodiments, the cancer is head and neck cancer. In some

embodiments, the head and neck cancer is squamous cell carcinoma of the head and neck.

[0024] In some embodiments, methods of identifying a subject with cancer who may benefit from administration of an FGFR1 ECD or FGFR1 ECD fusion molecule are provided. In some embodiments, a method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject comprise an FGFR1 gene amplification, wherein FGFR1 gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule. In some embodiments, FGFR1 gene amplification is determined by a method selected from fluorescence in situ hybridization, array comparative genomic hybridization, DNA microarray, spectral karyotyping, quantitative PCR, southern blotting, or sequencing.

[0025] In some embodiments, methods of identifying a subject with cancer who may benefit from administration of an FGFR1 ECD or FGFR1 ECD fusion molecule are provided. In some embodiments, a method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject overexpress at least one, at least two, at least three, at least four, or at least five markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4, wherein overexpression is indicative of therapeutic

responsiveness by the cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule. In some embodiments, the method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject overexpress at least one, at least two, at least three, or at least four markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, and FGF18. In some embodiments, the method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject overexpress ETV4. In some embodiments, including any of the foregoing embodiments, the method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject overexpress Gene 1 and Gene 2 from any line in Table 10 below, or any comination thereof. In some embodiments, FGFR1 is FGFRlIIIc. In some embodiments, the overexpression is mRNA overexpression. In some embodiments, mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the overexpression is protein overexpression. In some embodiments, protein overexpression is determined by immunohistochemistry. In some embodiments, including any of the foregoing embodiments, the method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject have an FGFRl gene amplification.

[0026] In some embodiments, methods of identifying a subject with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule are provided. In some embodiments, a method comprises determining whether at least a portion of the cancer cells in a sample obtained from the subject overexpress FGF2, wherein overexpression is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, the overexpression is mRNA overexpression. In some embodiments, mRNA overexpression is determined by quantitative RT-PCR. In some embodiments, the overexpression is protein overexpression. In some embodiments, protein overexpression is determined by immunohistochemistry. In some embodiments, the cancer is determined not to have an FGFRl gene amplification. In some embodiments, the cancer is lung cancer. In some embodiments, the cancer is non-small cell lung cancer or small cell lung cancer.

[0027] Any embodiment described herein or any combination thereof applies to any and all methods of the invention described herein.

BRIEF DESCRIPTION OF THE DRAWINGS

[0028] FIG. 1 shows cell number in a culture of (A) NCI-H 1581 , (B) NCI-H520, (C) DMS53, and (D) DMS1 14 tumor cells grown in the presence or absence of FGFR1- ECD.339-Fc, with varying amounts of serum, as described in Example 1.

[0029] FIG. 2 shows thymidine incorporation by (A) NCI-H 1581 , (B) NCI-H520, (C) DMS53, and (D) DMS1 14 tumor cells grown in the presence or absence of FGFR1- ECD.339-Fc, with varying amounts of serum, as described in Example 1.

[0030] FIG. 3 shows a plot of average % decrease in cell number in various FGFRl gene amplified lung cancer cell lines and various FGFRl gene non-amplified lung cancer cell lines grown in the presence of FGFR1-ECD.339-Fc, as described in Example 1.

[0031] FIG. 4 shows a plot of average % reduction in 3H-thymidine incorporation in various FGFRl gene amplified lung cancer cell lines and various FGFRl gene non-amplified lung cancer cell lines grown in the presence of FGFR1-ECD.339-Fc, as described in Example 1.

[0032] FIG. 5 shows mean tumor volume at various time points in mice implanted with DMS53 cells and treated with FGFR1-ECD.339-Fc or albumin, as described in Example 2.

[0033] FIG. 6 shows mean tumor volume at various time points in mice implanted with DMS114 cells and treated with FGFR1-ECD.339-Fc or albumin, as described in Example 3.

[0034] FIG. 7 shows mean tumor volume at various time points in mice implanted with NCI-H1581 cells and treated with FGFR1-ECD.339-Fc or albumin, as described in Example 4.

[0035] FIG. 8 shows mean tumor volume at various time points in mice implanted with NCI-H520 cells and treated with FGFR1-ECD.339-Fc or albumin, as described in Example 5.

[0036] FIG. 9 shows % tumor growth inhibition by FGFR1-ECD.339-Fc in mouse xenografts of tumor cells having FGFR1 gene amplification and tumor cells having a non- amplified FGFR1 gene, as described in Example 6.

[0037] FIG. 10 shows a scatter plot of FGFR1 mRNA expression in lung cancer cell lines with and without FGFR1 gene amplification, as described in Example 7.

[0038] FIG. 11 shows graphs of (A) average luminescence in the CellTiterGlo® assay and (B) counts per minute in the tritiated thymidine incorporation assay carried out on NCI-H226 cells grown with varying amounts of serum and in the presence or absence of FGFR1- ECD.339-Fc, as described in Example 7.

[0039] FIG. 12 shows a scatter plot of FGFR1 mRNA expression in lung cancer xenografts with and without FGFR1 gene amplification, as described in Example 7.

[0040] FIG. 13 shows mean tumor volume at various time points in mice implanted with PDX D35087 cells and treated with FGFR1-ECD.339-Fc or albumin, as described in

Example 7.

[0041] FIG. 14 shows (A) FGF2 mRNA (normalized to GUSB) and (B) FGF2 protein expression (normalized to total protein) in FGFR1-ECD.339-Fc responder and non-responder xenografts, as described in Example 8.

[0042] FIG. 15 shows DKK3 mRNA expression (normalized to GUSB) in FGFR1- ECD.339-Fc responder and non-responder xenografts, as described in Example 9.

[0043] FIG. 16 shows anti-tumor activity of FGFR1-ECD.339-Fc in (A) a Caki-1 renal cell carcinoma xenograft model, and (B) a MSTO-21 1H mesothelioma xenograft model, as described in Example 8. [0044] FIG. 17 shows (A) FGFRl and (B) FGFR3IIIc mRNA expression in FGFR1- ECD.339-Fc responsive and non-responsive xenograft models, as described in Example 8.

[0045] FIG. 18 shows (A) plasma FGFR1-ECD.339-Fc levels over time in rats administered weekly doses of FGFR1-ECD.339-Fc, and (B) serum phosphate levels after 24 hours and 168 hours in rats administered FGFR1-ECD.339-Fc or FGFR kinase inhibitor PD 173074, as described in Example 10.

[0046] FIG. 19 shows FGFR1-ECD.339-Fc mediated inhibition of FGF-2 and VEGF-A induced angiogenesis in a matrigel plug assay, as described in Example 1 1.

[0047] FIG. 20 shows that FGFR1-ECD.339-Fc does not inhibit VEGF-A induced human umbilical vein endothelial cell (HUVEC) proliferation, as described in Example 11.

[0048] FIG. 21 shows inhibition of tumor angiogenesis (as assessed by CD31 immunostaining) in Caki-1 renal cell carcinoma xenograft model mice administered FGFRl - ECD.339-Fc, as described in Example 12.

[0049] FIG. 22 shows FGFR1-ECD.339-Fc mediated inhibition of FGFRl signaling in a JIMT-1 breast cancer xenograft, as described in Example 13.

DETAILED DESCRIPTION

[0050] The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described.

Definitions

[0051] Unless otherwise defined, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by those of ordinary skill in the art. Further, unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.

[0052] Certain techniques used in connection with recombinant DNA, oligonucleotide synthesis, tissue culture and transformation (e.g., electroporation, lipofection), enzymatic reactions, and purification techniques are known in the art. Many such techniques and procedures are described, e.g., in Sambrook et al. Molecular Cloning: A Laboratory Manual (2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)), among other places. In addition, certain techniques for chemical syntheses, chemical analyses, pharmaceutical preparation, formulation, and delivery, and treatment of patients are also known in the art. [0053] In this application, the use of "or" means "and/or" unless stated otherwise. In the context of a multiple dependent claim, the use of "or" refers back to more than one preceding independent or dependent claim in the alternative only. Also, terms such as "element" or "component" encompass both elements and components comprising one unit and elements and components that comprise more than one subunit unless specifically stated otherwise.

[0054] As used herein, all numbers are approximate, and may be varied to account for measurement error and the rounding of significant digits. The use of "about" before certain measured quantities includes variations due to sample impurities, measurement error, human error, and statistical variation, as well as the rounding of significant digits.

[0055] As utilized in accordance with the present disclosure, the following terms, unless otherwise indicated, shall be understood to have the following meanings:

[0056] The terms "nucleic acid molecule" and "polynucleotide" may be used interchangeably, and refer to a polymer of nucleotides. Such polymers of nucleotides may contain natural and/or non-natural nucleotides, and include, but are not limited to, DNA, RNA, and PNA. "Nucleic acid sequence" refers to the linear sequence of nucleotides that comprise the nucleic acid molecule or polynucleotide.

[0057] The terms "polypeptide" and "protein" are used interchangeably to refer to a polymer of amino acid residues, and are not limited to a minimum length. Such polymers of amino acid residues may contain natural or non-natural amino acid residues, and include, but are not limited to, peptides, oligopeptides, dimers, trimers, and multimers of amino acid residues. Both full-length proteins and fragments thereof are encompassed by the definition. The terms also include post-expression modifications of the polypeptide, for example, glycosylation, sialylation, acetylation, phosphorylation, and the like. Furthermore, for purposes of the present invention, a "polypeptide" refers to a protein which includes modifications, such as deletions, additions, and substitutions (generally conservative in nature), to the native sequence, as long as the protein maintains the desired activity. These modifications may be deliberate, as through site-directed mutagenesis, or may be accidental, such as through mutations of hosts which produce the proteins or errors due to PCR amplification. When a polypeptide "consists of a particular amino acid sequence, it may still contain post-translational modifications, such as glycosylation and sialylation.

[0058] The term "FGFR1 extracellular domain" ("FGFR1 ECD") includes full-length FGFR1 ECDs, FGFR1 ECD fragments, and FGFR1 ECD variants. As used herein, the term "FGFR1 ECD" refers to an FGFR1 polypeptide that lacks the intracellular and

transmembrane domains, with or without a signal peptide. In some embodiment, the FGFR1 ECD is a human full-length FGFRl ECD having an amino acid sequence selected from SEQ ID NOs: 1 and 2. The term "full-length FGFRl ECD", as used herein, refers to an FGFRl ECD that extends to the last amino acid of the extracellular domain, and may or may not include an N-terminal signal peptide. As defined herein, the last amino acid of the full-length FGFRl ECD is at position 353. Thus, a human full-length FGFRl ECD may consist of the amino acid sequence corresponding to SEQ ID NO.: 2 (mature form) or to SEQ ID NO.: 1 (with the signal peptide). As used herein, the term "FGFRl ECD fragment" refers to an FGFRl ECD having one or more residues deleted from the N and/or C terminus of the full- length ECD and that retains the ability to bind to FGF-2. The FGFRl ECD fragment may or may not include an N-terminal signal peptide. In some embodiments, the FGFRl ECD fragment is a human FGFRl ECD fragment having an amino acid sequence corresponding to SEQ ID NO.: 4 (mature form) or to SEQ ID NO.: 3 (with the signal peptide).

[0059] As used herein, the term "FGFRl ECD variants" refers to FGFRl ECDs that contain amino acid additions, deletions, and substitutions and that remain capable of binding to FGF-2. Such variants may be at least 90%, 92%, 95%, 97%, 98%, or 99% identical to the parent FGFRl ECD. The % identity of two polypeptides can be measured by a similarity score determined by comparing the amino acid sequences of the two polypeptides using the Bestfit program with the default settings for determining similarity. Bestfit uses the local homology algorithm of Smith and Waterman, Advances in Applied Mathematics 2:482-489 (1981) to find the best segment of similarity between two sequences. In some embodiments, an FGFRl ECD variant is at least 95% identical to the sequence of SEQ ID NO: 4.

[0060] A polypeptide having an amino acid sequence at least, for example, 95% identical to a reference amino acid sequence of an FGFRl ECD polypeptide is one in which the amino acid sequence of the polypeptide is identical to the reference sequence except that the polypeptide sequence may include up to five amino acid alterations per each 100 amino acids of the reference polypeptide. In other words, to obtain a polypeptide having an amino acid sequence at least 95% identical to a reference amino acid sequence, up to 5% of the amino acid residues in the reference sequence may be deleted or substituted with another amino acid, or a number of amino acids, up to 5% of the total amino acid residues in the reference sequence, may be inserted into the reference sequence. These alterations of the reference sequence may occur at the N- or C- terminal positions of the reference amino acid sequence or anywhere between those terminal positions, interspersed either individually among residues in the reference sequence, or in one or more contiguous groups within the reference sequence. [0061] As a practical matter, whether any particular polypeptide is at least 70%, 80%, 90%, or 95% identical to, for instance, an amino acid sequence or to a polypeptide sequence encoded by a nucleic acid sequence set forth in the Sequence Listing can be determined conventionally using known computer programs, such the Bestfit program. When using Bestfit or other sequence alignment program to determine whether a particular sequence is, for instance, 95% identical to a reference sequence according to the present invention, the parameters are set, of course, that the percentage of identity is calculated over the full length of the reference amino acid sequence and that gaps in homology of up to 5% of the total number of amino acid residues in the reference sequence are allowed.

[0062] As used herein, the terms "hFGFRl-ECD.353" and "hFGFRl.353" may be used interchangeably to refer to the full-length human FGFRl ECD corresponding to SEQ ID NO: 1 (with signal peptide) or to SEQ ID NO: 2 (without signal peptide; mature form).

[0063] As used herein, the terms "hFGFRl-ECD.339" and "hFGFRl.339" may be used interchangeably to refer to the human FGFRl ECD corresponding to SEQ ID NO: 3 (with signal peptide) or to SEQ ID NO: 4 (without signal peptide; mature form).

[0064] Additional hFGFRl ECDs are described, for example, in U.S. Patent No.

7,678,890, which is incorporated by reference herein in its entirety for any purpose.

[0065] The term "FGFRl ECD fusion molecule" refers to a molecule comprising an FGFRl ECD, and one or more "fusion partners." In some embodiments, the FGFRl ECD and the fusion partner are covalently linked ("fused"). If the fusion partner is also a polypeptide ("the fusion partner polypeptide"), the FGFRl ECD and the fusion partner polypeptide may be part of a continuous amino acid sequence, and the fusion partner polypeptide may be linked to either the N terminus or the C terminus of the FGFRl ECD. In such cases, the FGFRl ECD and the fusion partner polypeptide may be translated as a single polypeptide from a coding sequence that encodes both the FGFRl ECD and the fusion partner polypeptide (the "FGFRl ECD fusion protein"). In some embodiments, the FGFRl ECD and the fusion partner are covalently linked through other means, such as, for example, a chemical linkage other than a peptide bond. Many known methods of covalently linking polypeptides to other molecules (for example, fusion partners) may be used. In other embodiments, the FGFRl ECD and the fusion partner may be fused through a "linker," which is comprised of at least one amino acid or chemical moiety.

[0066] In some embodiments, the FGFRl ECD polypeptide and the fusion partner are noncovalently linked. In some such embodiments, they may be linked, for example, using binding pairs. Exemplary binding pairs include, but are not limited to, biotin and avidin or streptavidin, an antibody and its antigen, etc.

[0067] Exemplary fusion partners include, but are not limited to, an immunoglobulin Fc domain, albumin, and polyethylene glycol. The amino acid sequences of some exemplary Fc domains are shown in SEQ ID NOs: 8 to 10. In some embodiments, an FGFRl ECD fused to an Fc is referred to as an "hFGFRl ECD-Fc." In some embodiments, the Fc domain is selected from an IgGl Fc, an IgG2 Fc, an IgG3 Fc, and an IgG4 Fc.

[0068] As used herein, the terms "hFGFRl-ECD.339-Fc" and "hFGFRl .339-Fc" may be used interchangeably to refer to an amino acid sequence selected from SEQ ID NO: 6 (without signal peptide, mature form) and SEQ ID NO: 5 (with signal peptide). Nonlimiting exemplary cancers that may be treated with hFGFRl-ECD.339-Fc include, but are not limited to, lung cancer, colon cancer, breast cancer, gastric cancer, head and neck cancer, prostate cancer, endometrial cancer, sarcoma, small cell lung cancer, ovarian cancer, Kaposi's sarcoma, Hodgkin's disease, leukemia, non-Hodgkin's lymphoma, neuroblastoma (brain cancer), rhabdomyosarcoma, Wilms' tumor, acute lymphoblastic leukemia, acute

lymphoblastic leukemia, bladder cancer, testicular cancer, lymphomas, germ cell tumors, cancers of the colon and rectum, gastrointestinal cancers, thyroid cancer, multiple myeloma, pancreatic cancer, mesothelioma, malignant pleural mesothelioma, hematological/lymphatic cancers, malignant peritoneal mesothelioma, esophageal cancer, renal cell carcinoma, glioblastoma multiforme, and liver cancer.

[0069] The term "signal peptide" refers to a sequence of amino acid residues located at the N terminus of a polypeptide that facilitates secretion of a polypeptide from a mammalian cell. A signal peptide may be cleaved upon export of the polypeptide from the mammalian cell, forming a mature protein. Signal peptides may be natural or synthetic, and they may be heterologous or homologous to the protein to which they are attached. Exemplary signal peptides include, but are not limited to, FGFRl signal peptides, such as, for example, the amino acid sequence of SEQ ID NO: 7. Exemplary signal peptides also include signal peptides from heterologous proteins. A "signal sequence" refers to a polynucleotide sequence that encodes a signal peptide. In some embodiments, an FGFRl ECD lacks a signal peptide. In some embodiments, an FGFRl ECD includes at least one signal peptide, which may be a native FGFRl signal peptide or a heterologous signal peptide.

[0070] The term "vector" is used to describe a polynucleotide that may be engineered to contain a cloned polynucleotide or polynucleotides that may be propagated in a host cell. A vector may include one or more of the following elements: an origin of replication, one or more regulatory sequences (such as, for example, promoters and/or enhancers) that regulate the expression of the polypeptide of interest, and/or one or more selectable marker genes (such as, for example, antibiotic resistance genes and genes that may be used in colorimetric assays, e.g., β-galactosidase). The term "expression vector" refers to a vector that is used to express a polypeptide of interest in a host cell.

[0071] A "host cell" refers to a cell that may be or has been a recipient of a vector or isolated polynucleotide. Host cells may be prokaryotic cells or eukaryotic cells. Exemplary eukaryotic cells include mammalian cells, such as primate or non-primate animal cells;

fungal cells; plant cells; and insect cells. Exemplary mammalian cells include, but are not limited to, 293 and CHO cells, and their derivatives, such as 293-6E and DG44 cells, respectively.

[0072] The term "isolated" as used herein refers to a molecule that has been separated from at least some of the components with which it is typically found in nature. For example, a polypeptide is referred to as "isolated" when it is separated from at least some of the components of the cell in which it was produced. Where a polypeptide is secreted by a cell after expression, physically separating the supernatant containing the polypeptide from the cell that produced it is considered to be "isolating" the polypeptide. Similarly, a

polynucleotide is referred to as "isolated" when it is not part of the larger polynucleotide (such as, for example, genomic DNA or mitochondrial DNA, in the case of a DNA polynucleotide) in which it is typically found in nature, or is separated from at least some of the components of the cell in which it was produced, e.g., in the case of an RNA

polynucleotide. Thus, a DNA polynucleotide that is contained in a vector inside a host cell may be referred to as "isolated" so long as that polynucleotide is not found in that vector in nature.

[0073] The term "anti-neoplastic composition" refers to a composition useful in treating cancer comprising at least one active therapeutic agent, e.g., an "anti-cancer agent."

Examples of therapeutic agents (anti-cancer agents) include, but are not limited to, e.g., chemotherapeutic agents, growth inhibitory agents, cytotoxic agents, agents used in radiation therapy, anti-angiogenic agents, apoptotic agents, anti-tubulin agents, and other agents to treat cancer, such as anti-VEGF antibodies (e.g., bevacizumab, AVASTIN^®), anti-HER-2 antibodies (e.g., trastuzumab, HERCEPTIN^®), anti-CD20 antibodies (e.g., rituximab, RITUXAN^®), an epidermal growth factor receptor (EGFR) antagonist (e.g., a tyrosine kinase inhibitor), HER 1 /EGFR inhibitors (e.g., erlotinib, TARCEVA^®), platelet derived growth factor inhibitors (e.g., GLEEVEC^®, imatinib mesylate)), COX-2 inhibitors (e.g., celecoxib), interferons, cytokines, antagonists (e.g., neutralizing antibodies) that bind to one or more of the following targets ErbB2, ErbB3, ErbB4, PDGFR-beta, BlyS, APRIL, BCMA or VEGF receptor(s), TRAIL/Apo2, and other bioactive and organic chemical agents, etc.

Combinations thereof are also included in the invention.

[0074] A "chemotherapeutic agent" refers to a chemical compound useful in the treatment of cancer. Examples of chemotherapeutic agents include alkylating agents such as thiotepa and cyclophosphamide (CYTOXAN®); alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphoramide and trimethylomelamine;

acetogenins (especially bullatacin and bullatacinone); delta-9-tetrahydrocannabinol (dronabinol, MARTNOL®); beta-lapachone; lapachol; colchicines; betulinic acid; a camptothecin (including the synthetic analogue topotecan (HYCAMTIN®), CPT- 11 (irinotecan, CAMPTOSAR®), acetylcamptothecin, scopolectin, and 9-aminocamptothecin); bryostatin; callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogues); podophyllotoxin; podophyllinic acid; teniposide; cryptophycins (particularly cryptophycin 1 and cryptophycin 8); dolastatin; duocarmycin (including the synthetic analogues, KW-2189 and CB 1-TM1); eleutherobin; pancratistatin; a sarcodictyin;

spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, chlorophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard;

nitrosoureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimnustine; antibiotics such as the enediyne antibiotics (e. g., calicheamicin, especially calicheamicin gammall and calicheamicin omegall (see, e.g., Nicolaou et al., Angew. Chem Intl. Ed. Engl, 33 : 183-186 (1994)); CDP323, an oral alpha-4 integrin inhibitor; dynemicin, including dynemicin A; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores), aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, carminomycin, carzinophilin, chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin (including ADRIAMYCIN®, morpholino-doxorubicin, cyanomorpholino- doxorubicin, 2-pyrrolino-doxorubicin, doxorubicin HC1 liposome injection (DOXIL®), liposomal doxorubicin TLC D-99 (MYOCET®), pegylated liposomal doxorubicin

(CAELYX®), and deoxydoxorubicin), epirubicin, esorubicin, idarubicin, marcellomycin, mitomycins such as mitomycin C, mycophenolic acid, nogalamycin, olivomycins, peplomycin, porfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate, gemcitabine (GEMZAR®), pemetrexed (ALIMTA®); tegafur (UFTORAL®), capecitabine (XELODA®), an epothilone, and 5-fluorouracil (5-FU); folic acid analogues such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogs such as fludarabine, 6- mercaptopurine, thiamiprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elfornithine; elliptinium acetate; an epothilone; etoglucid; gallium nitrate;

hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; nitraerine; pentostatin; phenamet; pirarubicin; losoxantrone; 2-ethylhydrazide; procarbazine; PSK® polysaccharide complex (JHS Natural Products, Eugene, OR); razoxane; rhizoxin; sizofiran; spirogermanium; tenuazonic acid; triaziquone; 2,2',2'-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine); urethan; vindesine (ELDISINE®, FILDESIN®); dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside ("Ara-C"); thiotepa; taxoid, e.g., paclitaxel (TAXOL®), albumin-engineered nanoparticle formulation of paclitaxel (ABRAXANE™), and docetaxel (TAXOTERE®); chloranbucil; 6-thioguanine; mercaptopurine; methotrexate; platinum agents such as cisplatin, oxaliplatin (e.g.,

ELOXATIN®), and carboplatin; vincas, which prevent tubulin polymerization from forming microtubules, including vinblastine (VELBAN®), vincristine (ONCOVIN®), vindesine (ELDISINE®, FILDESIN®), and vinorelbine (NAVELBINE®); etoposide (VP-16);

ifosfamide; mitoxantrone; leucovorin; novantrone; edatrexate; daunomycin; aminopterin; ibandronate; topoisomerase inhibitor RFS 2000; difluoromethylornithine (DMFO); retinoids such as retinoic acid, including bexarotene (TARGRETiN®); bisphosphonates such as clodronate (for example, BONEFOS® or OSTAC®), etidronate (DIDROCAL®), NE-58095, zoledronic acid/zoledronate (ZOMETA®), alendronate (FOSAMAX®), pamidronate (AREDIA®), tiludronate (SKELID®), or risedronate (ACTONEL®); troxacitabine (a 1,3- dioxolane nucleoside cytosine analog); antisense oligonucleotides, particularly those that inhibit expression of genes in signaling pathways implicated in aberrant cell proliferation, such as, for example, PKC-alpha, Raf, H-Ras, and epidermal growth factor receptor (EGF- R); vaccines such as THERATOPE® vaccine and gene therapy vaccines, for example, ALLOVECTIN® vaccine, LEUVECTIN® vaccine, and VAXID® vaccine; topoisomerase 1 inhibitor (e.g., LURTOTECAN®); rmRH (e.g., ABARELIX®); BAY439006 (sorafenib, NEXAVAR^®; Bayer); SU- 1 1248 (sunitinib, SUTENT®, Pfizer); perifosine, COX-2 inhibitor (e.g. celecoxib or etoricoxib), proteosome inhibitor (e.g. PS341); bortezomib (VELCADE®); CCI-779; tipifarnib (R11577); orafenib, ABT510; Bcl-2 inhibitor such as oblimersen sodium (GENASENSE®); pixantrone; EGFR inhibitors (see definition below); tyrosine kinase inhibitors (see definition below); serine-threonine kinase inhibitors such as rapamycin (sirolimus, RAPAMUNE®); farnesyltransferase inhibitors such as lonafarnib (SCH 6636, SARASAR™); and pharmaceutically acceptable salts, acids or derivatives of any of the above; as well as combinations of two or more of the above such as CHOP, an abbreviation for a combined therapy of cyclophosphamide, doxorubicin, vincristine, and prednisolone; and FOLFOX, an abbreviation for a treatment regimen with oxaliplatin (ELOXATIN®) combined with 5-FU and leucovorin.

[0075] Chemotherapeutic agents as defined herein include "anti-hormonal agents" or "endocrine therapeutics" which act to regulate, reduce, block, or inhibit the effects of hormones that can promote the growth of cancer. They may be hormones themselves, including, but not limited to: anti-estrogens with mixed agonist/antagonist profile, including, tamoxifen (NOLVADEX®), 4-hydroxytamoxifen, toremifene (FARESTON®), idoxifene, droloxifene, raloxifene (EVISTA®), trioxifene, keoxifene, and selective estrogen receptor modulators (SERMs) such as SERM3; pure anti-estrogens without agonist properties, such as fulvestrant (FASLODEX®), and EM800 (such agents may block estrogen receptor (ER) dimerization, inhibit DNA binding, increase ER turnover, and/or suppress ER levels);

aromatase inhibitors, including steroidal aromatase inhibitors such as formestane and exemestane (AROMASIN®), and nonsteroidal aromatase inhibitors such as anastrazole (ARIMIDEX®), letrozole (FEMARA®) and aminoglutethimide, and other aromatase inhibitors include vorozole (RIVISOR®), megestrol acetate (MEGASE®), fadrozole, and 4(5)-imidazoles; lutenizing hormone-releasing hormone agonists, including leuprolide (LUPRON® and ELIGARD®), goserelin, buserelin, and tripterelin; sex steroids, including progestins such as megestrol acetate and medroxyprogesterone acetate, estrogens such as diethylstilbestrol and premarin, and androgens/retinoids such as fluoxymesterone, all transretinoic acid and fenretinide; onapristone; anti-progesterones; estrogen receptor down- regulators (ERDs); anti-androgens such as flutamide, nilutamide and bicalutamide; and pharmaceutically acceptable salts, acids or derivatives of any of the above; as well as combinations of two or more of the above.

[0076] An "angiogenic factor or agent" refers to a growth factor which stimulates the development of blood vessels, e.g., promote angiogenesis, endothelial cell growth, stability of blood vessels, and/or vasculogenesis, etc. For example, angiogenic factors, include, but are not limited to, e.g., VEGF and members of the VEGF family (VEGF-B, VEGF-C and VEGF- D), P1GF, PDGF family, fibroblast growth factor family (FGFs), TIE ligands

(Angiopoietins), ephrins, delta-like ligand 4 (DLL4), del- 1 , fibroblast growth factors: acidic (aFGF) and basic (bFGF), follistatin, granulocyte colony-stimulating factor (G-CSF), hepatocyte growth factor (HGF) /scatter factor (SF), interleukin-8 (IL-8), leptin, midkine, neuropilins, placental growth factor, platelet-derived endothelial cell growth factor (PD- ECGF), platelet-derived growth factor, especially PDGF-BB or PDGFR-beta, pleiotrophin (PTN), progranulin, proliferin, transforming growth factor-alpha (TGF-alpha), transforming growth factor-beta (TGF-beta), tumor necrosis factor-alpha (TNF-alpha), etc. It would also include factors that accelerate wound healing, such as growth hormone, insulin-like growth factor-I (IGF-I), VIGF, epidermal growth factor (EGF), CTGF and members of its family, and TGF-alpha and TGF-beta. See, e.g., Klagsbrun and D'Amore (1991) Annu. Rev. Physiol. 53:217-39; Streit and Detmar (2003) Oncogene 22:3172-3179; Ferrara & Alitalo (1999) Nature Medicine 5(12): 1359-1364; Tonini et al. (2003) Oncogene 22:6549-6556 {e.g., Table 1 listing known angiogenic factors); and, Sato (2003) Int. J. Clin. Oncol. 8:200-206.

[0077] An "anti-angiogenic agent" or "angiogenesis inhibitor" refers to a small molecular weight substance, a polynucleotide (including, e.g., an inhibitory R A (R Ai or siRNA)), a polypeptide, an isolated protein, a recombinant protein, an antibody, or conjugates or fusion proteins thereof, that inhibits angiogenesis, vasculogenesis, or undesirable vascular permeability, either directly or indirectly. It should be understood that the anti-angiogenic agent includes those agents that bind and block the angiogenic activity of the angiogenic factor or its receptor. For example, an anti-angiogenic agent is an antibody or other antagonist to an angiogenic agent as defined above, e.g., fusion proteins that binds to VEGF- A such as ZALTRAP™ (Aflibercept), antibodies to VEGF-A such as AVASTIN^®

(bevacizumab) or to the VEGF-A receptor (e.g., KDR receptor or Flt-1 receptor), anti- PDGFR inhibitors such as GLEEVEC^® (Imatinib Mesylate), small molecules that block VEGF receptor signaling (e.g., PTK787/ZK2284, SU6668, SUTENT®/SU 11248 (sunitinib malate), AMG706, or those described in, e.g., international patent application WO

2004/1 13304). Anti-angiogenic agents also include native angiogenesis inhibitors, e.g., angiostatin, endostatin, etc. See, e.g., Klagsbrun and D'Amore (1991) Annu. Rev. Physiol. 53:217-39; Streit and Detmar (2003) Oncogene 22:3172-3179 (e.g., Table 3 listing anti- angiogenic therapy in malignant melanoma); Ferrara & Alitalo (1999) Nature Medicine 5(12): 1359-1364; Tonini et al. (2003) Oncogene 22:6549-6556 (e.g., Table 2 listing known anti-angiogenic factors); and, Sato (2003) Int. J. Clin. dOncol. 8:200-206 (e.g., Table 1 listing anti-angiogenic agents used in clinical trials).

[0078] The term "VEGF" or "VEGF-A" as used herein refers to the 165-amino acid human vascular endothelial cell growth factor and related 121-, 189-, and 206- amino acid human vascular endothelial cell growth factors, as described by Leung et al. (1989) Science 246: 1306, and Houck et al. (1991) Mol. Endocrin, 5: 1806, together with the naturally occurring allelic and processed forms thereof. The term "VEGF" also refers to VEGFs from non-human species such as mouse, rat or primate. Sometimes the VEGF from a specific species are indicated by terms such as hVEGF for human VEGF, mVEGF for murine VEGF, and etc. The term "VEGF" is also used to refer to truncated forms of the polypeptide comprising amino acids 8 to 109 or 1 to 109 of the 165-amino acid human vascular endothelial cell growth factor. Reference to any such forms of VEGF may be identified in the present application, e.g., by "VEGF (8-109)," "VEGF (1-109)," "VEGF-A₁₀₉" or "VEGF 165." The amino acid positions for a "truncated" native VEGF are numbered as indicated in the native VEGF sequence. For example, amino acid position 17 (methionine) in truncated native VEGF is also position 17 (methionine) in native VEGF. The truncated native VEGF has binding affinity for the KDR and Fit- 1 receptors comparable to native VEGF.

[0079] A "VEGF antagonist" refers to a molecule capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF activities including, but not limited to, its binding to one or more VEGF receptors. VEGF antagonists include, without limitation, anti-VEGF antibodies and antigen-binding fragments thereof, receptor molecules and derivatives which bind specifically to VEGF thereby sequestering its binding to one or more receptors, anti-VEGF receptor antibodies, VEGF receptor antagonists such as small molecule inhibitors of the VEGFR tyrosine kinases (e.g., pazopanib) and immunoadhesins that binds to VEGF such as VEGF trap (e.g., aflibercept). The term "VEGF antagonist," as used herein, specifically includes molecules, including antibodies, antibody fragments, other binding polypeptides, peptides, and non-peptide small molecules, that bind to VEGF and are capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF activities. Thus, the term "VEGF activities" specifically includes VEGF mediated biological activities of VEGF.

[0080] The term "VEGF trap" as used herein means a protein, such as a fusion molecule, that binds to VEGF and is capable of neutralizing, blocking, inhibiting, abrogating, reducing or interfering with VEGF activities. An example of a VEGF trap is aflibercept.

[0081] The term "anti-VEGF antibody" or "an antibody that binds to VEGF" refers to an antibody that is capable of binding to VEGF with sufficient affinity and specificity that the antibody is useful as a diagnostic and/or therapeutic agent in targeting VEGF. Anti-VEGF neutralizing antibodies suppress the growth of a variety of human tumor cell lines in nude mice (Kim et al, Nature 362:841-844 (1993); Warren et al, J. Clin. Invest. 95: 1789-1797 (1995); Borgstrom et al, Cancer Res. 56:4032-4039 (1996); Melnyk ei a/., Cancer Res. 56:921-924 (1996)) and also inhibit intraocular angiogenesis in models of ischemic retinal disorders. Adamis et al, Arch. Ophthalmol. 1 14:66-71 (1996). For example, the anti-VEGF antibody can be used as a therapeutic agent in targeting and interfering with diseases or conditions wherein the VEGF activity is involved. See, e.g., U.S. Patents 6,582,959, 6,703,020; W098/45332; WO 96/30046; WO94/10202, WO2005/044853; EP 0666868B1 ; US Patent Applications 20030206899, 20030190317, 20030203409, 200501 12126,

20050186208, and 20050112126; Popkov et al, Journal of Immunological Methods 288: 149- 164 (2004); and WO2005012359. The antibody selected will normally have a sufficiently strong binding affinity for VEGF. For example, the antibody may bind hVEGF with a ¾ value of between 100 nM-1 pM. Antibody affinities may be determined by a surface plasmon resonance based assay (such as the BIAcore assay as described in PCT Application Publication No. WO2005/012359); enzyme-linked immunoabsorbent assay (ELISA); and competition assays (e.g. RIA's), for example. The antibody may be subjected to other biological activity assays, e.g., in order to evaluate its effectiveness as a therapeutic. Such assays are known in the art and depend on the target antigen and intended use for the antibody. Examples include the HUVEC inhibition assay; tumor cell growth inhibition assays (as described in WO 89/06692, for example); antibody-dependent cellular cytotoxicity (ADCC) and complement-mediated cytotoxicity (CDC) assays (US Patent 5,500,362); and agonistic activity or hematopoiesis assays (see WO 95/27062). An anti-VEGF antibody will usually not bind to other VEGF homologues such as VEGF-B, VEGF-C, VEGF-D or VEGF- E, nor other growth factors such as P1GF, PDGF or bFGF.

[0082] In one embodiment, anti-VEGF antibodies include a monoclonal antibody that binds to the same epitope as the monoclonal anti-VEGF antibody A4.6.1 produced by hybridoma ATCC HB 10709; a recombinant humanized anti-VEGF monoclonal antibody (see Presta et al. (1997) Cancer Res. 57:4593-4599), including but not limited to the antibody known as "bevacizumab" also known as "rhuMAb VEGF" or "AVASTIN^®." AVASTI ^® is presently commercially available. Nonlimiting exemplary cancers that may be treated with bevacizumab include non-small cell lung cancer, colorectal cancer, breast cancer, renal cancer, ovarian cancer, glioblastoma multiforme, pediatric osteosarcoma, gastric cancer and pancreatic cancer. Bevacizumab comprises mutated human IgGi framework regions and antigen-binding complementarity-determining regions from the murine antibody A.4.6.1 that blocks binding of human VEGF to its receptors. Bevacizumab and other humanized anti- VEGF antibodies are further described in U.S. Pat. Nos. 6,884,879, and 7, 169,901.

Additional anti-VEGF antibodies are described in PCT Application Publication Nos.

WO2005/012359 and WO2009/073160; U.S. Pat. Nos. 7,060,269, 6,582,959, 6,703,020; 6,054,297; W098/45332; WO 96/30046; WO94/10202; EP 0666868B1 ; U.S. Patent Application Publication Nos. 2006009360, 20050186208, 20030206899, 20030190317, 20030203409, and 20050112126; and Popkov et al., Journal of Immunological Methods 288: 149-164 (2004).

[0083] The terms "subject" and "patient" are used interchangeably herein to refer to a mammal. In some embodiments, the subject or patient is a human. In other embodiments, methods of treating other mammals, including, but not limited to, rodents, simians, felines, canines, equines, bovines, porcines, ovines, caprines, mammalian laboratory animals, mammalian farm animals, mammalian sport animals, and mammalian pets, are also provided.

[0084] The term "sample" or "patient sample" as used herein, refers to a composition that is obtained or derived from a subject of interest that contains a cellular and/or other molecular entity that is to be characterized and/or identified, for example based on physical, biochemical, chemical and/or physiological characteristics. For example, the phrase "disease sample" and variations thereof refers to any sample obtained from a subject of interest that would be expected or is known to contain the cellular and/or molecular entity that is to be characterized. By "tissue or cell sample" is meant a collection of similar cells obtained from a tissue of a subject or patient. The source of the tissue or cell sample may be solid tissue as from a fresh, frozen and/or preserved organ or tissue sample or biopsy or aspirate; blood or any blood constituents; bodily fluids such as cerebral spinal fluid, amniotic fluid, peritoneal fluid, or interstitial fluid; cells from any time in gestation or development of the subject. The tissue sample may also be primary or cultured cells or cell lines. Optionally, the tissue or cell sample is obtained from a disease tissue/organ. The tissue sample may contain compounds which are not naturally intermixed with the tissue in nature such as preservatives, anticoagulants, buffers, fixatives, nutrients, antibiotics, or the like.

[0085] A "reference sample", "reference cell", or "reference tissue", as used herein, refers to a sample, cell or tissue obtained from a source known, or believed, not to be afflicted with the disease or condition for which a method or composition of the invention is being used to identify. In some embodiments, a reference sample, reference cell or reference tissue is obtained from a healthy part of the body of the same subject or patient in whom a disease or condition is being identified using a composition or method of the invention. In some embodiments, a reference sample, reference cell or reference tissue is obtained from a healthy part of the body of one or more individuals who are not the subject or patient in whom a disease or condition is being identified using a composition or method of the invention.

[0086] "Cancer" and "tumor," as used herein, are interchangeable terms that refer to any abnormal cell or tissue growth or proliferation in an animal. As used herein, the terms "cancer" and "tumor" encompass solid and hematological/lymphatic cancers and also encompass malignant, pre-malignant, and benign growth, such as dysplasia. Examples of cancer include but are not limited to, carcinoma, lymphoma, blastoma, sarcoma, and leukemia. More particular non-limiting examples of such cancers include squamous cell cancer, small-cell lung cancer, pituitary cancer, esophageal cancer, astrocytoma, soft tissue sarcoma, non-small cell lung cancer, adenocarcinoma of the lung, squamous carcinoma of the lung, cancer of the peritoneum, hepatocellular cancer, gastrointestinal cancer, pancreatic cancer, glioblastoma, cervical cancer, ovarian cancer, liver cancer, bladder cancer, hepatoma, breast cancer, colon cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney cancer, renal cancer, liver cancer, prostate cancer, vulval cancer, thyroid cancer, hepatic carcinoma, brain cancer, endometrial cancer, testis cancer, cholangiocarcinoma, gallbladder carcinoma, gastric cancer, melanoma, and various types of head and neck cancer.

[0087] The term "lung cancer," as used herein, refers to both small cell lung cancer and non-small cell lung cancers. Non-small cell lung cancer includes, but is not limited to, squamous cell lung cancer, adenocarcinoma, large-cell lung carcinoma, sarcomatoid carcinoma, carcinoid tumors, pulmonary pleomorphic carcinoma, and adenosquamous carcinoma and bronchioloalveolar carcinoma. Small cell lung cancer may, in some embodiments, be referred to as "oat-cell" cancer, and includes, but is not limited to, combined small-cell carcinoma, which comprises a mixture of small cell and non-small cell carcinomas. [0088] A "cell with FGFRl gene amplification" refers to a cell that comprises more than two copies of the FGFRl gene. In some embodiments, a cell with FGFRl gene amplification refers to a cell that has a ratio of FGFRl gene to chromosome 8 centromere of greater than 1. In some embodiments, the ratio is determined by fluorescence in situ hybridization. "Cancer with FGFRl gene amplification," as used herein, refers to a cancer in which at least a portion of the cancer cells have FGFRl gene amplification. In some embodiments, a cancer with FGFRl gene amplification refers to a cancer in which at least a portion of the cancer cells comprise at least four copies of the FGFRl gene. In some embodiments, a cancer with FGFRl gene amplification refers to a cancer in which at least a portion of the cancer cells have an FGFRl gene:chromosome 8 centromere ratio of greater than 1. An exemplary FGFRl gene sequence can be found, e.g., NCBI Reference Sequence: NG_007729.1 dated 25-MAR-2012.

[0089] In some embodiments, a cell with FGFRl gene amplification comprises at least 3 copies, at least 4 copies, at least 5 copies, at least 6 copies, at least 8 copies, or at least 10 copies of the FGFRl gene. In some embodiments, a cell with FGFRl gene amplification comprises at least 4 copies. In some embodiments, a cell with FGFRl gene amplification has a ratio of FGFRl gene: chromosome 8 centromere of at least 1.5, at least 2, at least 2.5, at least 3, at least 3.5, or at least 4. In some embodiments, a cell with FGFRl gene

amplification has a ratio of FGFRl gene: chromosome 8 centromere of at least 2. In some embodiments, each copy of the FGFRl gene in a cell with FGFRl gene amplification need not be a complete copy of the FGFRl gene. In some embodiments, a cell with FGFRl gene amplification has elevated levels of FGFRl (i.e., in some embodiments, a cell with FGFRl gene amplification is also a cell with FGFRl overexpression).

[0090] A "cell with FGFRl overexpression" or a "cell that overexpresses FGFRl" refers to a cell that has at least a 2-fold greater level of FGFRl mRNA or protein than a reference cell. A "cancer with FGFRl overexpression" or a "cancer that overexpresses FGFRl" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of FGFRl mRNA or protein than a reference cell. In some embodiments, a cell with FGFRl overexpression has at least 3-fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10-fold greater level of FGFRl mRNA or protein than a reference cell. The level of FGFRl mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. In some embodiments, FGFRl is FGFRlIIIc. An exemplary human FGFRl protein sequence can be found, e.g., at UniProtKB/Swiss-Prot Reference Sequence: PI 1362 (FGFR1_HUMAN) dated March 21, 2012. An exemplary human FGFR1 mRNA sequence can be found, e.g., at NCBI Reference Sequence:

NM_023110.2 dated 24-MAR-2012. An exemplary human FGFRlIIIc protein sequence can be found, e.g., at NCBI Reference Sequence: NP_075598.2 dated 24-MAR-2012. An exemplary human FGFRlIIIc mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_0231 10.2 dated 24-MAR-2012.

[0091] A "cell with FGFR3IIIc overexpression" or a "cell that overexpresses

FGFR3IIIc" refers to a cell that has at least a 2-fold greater level of FGFR3IIIc mRNA or protein than a reference cell. A "cancer with FGFR3IIIc overexpression" or a "cancer that overexpresses FGFR3IIIc" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of FGFR3IIIc mRNA or protein than a reference cell. In some embodiments, a cell with FGFR3IIIc overexpression has at least 3 -fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10-fold greater level of FGFR3IIIc mRNA or protein than a reference cell. The level of FGFR3IIIc mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. An exemplary human FGFR3IIIc protein sequence can be found, e.g., at NCBI Reference Sequence:

NP 000133.1 dated 12-FEB-2012. An exemplary human FGFR3IIIc mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_000142.4 dated 12-FEB-2012.

[0092] A "cell with FGF2 overexpression" or a "cell that overexpresses FGF2" refers to a cell that has at least a 2-fold greater level of FGF2 mRNA or protein than a reference cell. A "cancer with FGF2 overexpression" or a "cancer that overexpresses FGF2" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of FGF2 mRNA or protein than a reference cell. In some embodiments, a cell with FGF2

overexpression has at least 3-fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10- fold greater level of FGF2 mRNA or protein than a reference cell. The level of FGF2 mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. An exemplary human FGF2 protein sequence can be found, e.g., at NCBI Reference Sequence: NP_001997.5 dated 12-FEB-2012. An exemplary human FGF2 mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_002006.4 dated 12-FEB-2012.

[0093] A "cell with DKK3 overexpression" or a "cell that overexpresses DKK3" refers to a cell that has at least a 2-fold greater level of DKK3 mRNA or protein than a reference cell. A "cancer with DKK3 overexpression" or a "cancer that overexpresses DKK3" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of DKK3 mRNA or protein than a reference cell. In some embodiments, a cell with DKK3 overexpression has at least 3-fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10- fold greater level of DKK3 mRNA or protein than a reference cell. The level of DKK3 mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. An exemplary human DKK3 protein sequence can be found, e.g., at NCBI Reference Sequence: NP_001018067.1 dated 22-JAN-2012. An exemplary human DKK3 mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_001018057.1 dated 22-JAN-2012.

[0094] A "cell with FGF18 overexpression" or a "cell that overexpresses FGF18" refers to a cell that has at least a 2-fold greater level of FGF18 mRNA or protein than a reference cell. A "cancer with FGF18 overexpression" or a "cancer that overexpresses FGF18" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of FGF 18 mRNA or protein than a reference cell. In some embodiments, a cell with FGF18 overexpression has at least 3 -fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10-fold greater level of FGF 18 mRNA or protein than a reference cell. The level of FGF 18 mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. An exemplary human FGF 18 protein sequence can be found, e.g., at NCBI Reference Sequence: NP_003853 dated 27-JUN-2012. An exemplary human FGF 18 mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_003862.2 dated 27-JUN-2012.

[0095] A "cell with ETV4 overexpression" or a "cell that overexpresses ETV4" refers to a cell that has at least a 2-fold greater level of ETV4 mRNA or protein than a reference cell. A "cancer with ETV4 overexpression" or a "cancer that overexpresses ETV4" refers to a cancer in which at least a portion of the cells have at least a 2-fold greater level of ETV4 mRNA or protein than a reference cell. In some embodiments, a cell with ETV4 overexpression has at least 3-fold, at least 4-fold, at least 5-fold, at least 7-fold, or at least 10- fold greater level of ETV4 mRNA or protein than a reference cell. The level of ETV4 mRNA or protein can be determined by any suitable method including, but not limited to, the methods described herein. An exemplary human ETV4 protein sequence can be found, e.g., at NCBI Reference Sequence: NP_001977.1 dated 08-SEP-2012. An exemplary human ETV4 mRNA sequence can be found, e.g., at NCBI Reference Sequence: NM_001986.2 dated 08-SEP-2012.

[0096] "Treatment," as used herein, includes any administration or application of a therapeutic for condition in a mammal, including a human, and includes inhibiting the condition or progression of the condition, inhibiting or slowing the condition or its progression, arresting its development, partially or fully relieving the condition, or curing the condition, for example, by causing regression, or restoring or repairing a lost, missing, or defective function; or stimulating an inefficient process. In some embodiments, "treatment" refers to clinical intervention in an attempt to alter the natural course of the individual or cell being treated, and can be performed either for prophylaxis or during the course of clinical pathology. Desirable effects of treatment include preventing occurrence or recurrence of disease, alleviation of symptoms, diminishment of any direct or indirect pathological consequences of the disease, preventing metastasis, decreasing the rate of disease progression, amelioration or palliation of the disease state, and remission or improved prognosis.

[0097] An "effective amount" or "therapeutically effective amount" of a molecule or a combination of molecules means an amount that is sufficient to treat a condition and/or to inhibit growth of tumor cells in at least a subset of subjects when given alone or in combination with other treatments. In certain embodiments, a therapeutically effective amount refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired therapeutic or prophylactic result. A therapeutically effective amount of FGFR1 fusion protein of the invention may vary according to factors such as the disease state, age, sex, and weight of the individual, and the ability of FGFR1 fusion protein to elicit a desired response in the individual. A therapeutically effective amount is also one in which any toxic or detrimental effects of the FGFR1 fusion proteins are outweighed by the therapeutically beneficial effects. In the case of cancer, the effective amount of the drug may reduce the number of cancer cells; reduce the tumor size; inhibit (i.e., slow to some extent and typically stop) cancer cell infiltration into peripheral organs; inhibit (i.e., slow to some extent and typically stop) tumor metastasis; inhibit, to some extent, tumor growth; allow for treatment of the tumor, and/or relieve to some extent one or more of the symptoms associated with the disorder. To the extent the drug may prevent growth and/or kill existing cancer cells, it may be cytostatic and/or cytotoxic.

[0098] A "prophylactically effective amount" refers to an amount effective, at dosages and for periods of time necessary, to achieve the desired prophylactic result. Typically but not necessarily, since a prophylactic dose is used in subjects prior to or at an earlier stage of disease, the prophylactically effective amount will be less than the therapeutically effective amount.

[0099] The terms "inhibition" or "inhibit" refer to a decrease or cessation of any phenotypic characteristic or to the decrease or cessation in the incidence, degree, or likelihood of that characteristic. Nonlimiting exemplary inhibition includes inhibition of tumor growth.

[0100] The terms "benefit", "clinical benefit", "responsiveness", and "therapeutic responsiveness" as used herein in the context of benefiting from or responding to administration of a therapeutic agent, can be measured by assessing various endpoints, e.g., inhibition, to some extent, of disease progression, including slowing down and complete arrest; reduction in the number of disease episodes and/or symptoms; reduction in lesion size; inhibition (i.e., reduction, slowing down or complete stopping) of disease cell infiltration into adjacent peripheral organs and/or tissues; inhibition (i.e. reduction, slowing down or complete stopping) of disease spread; decrease of auto-immune response, which may, but does not have to, result in the regression or ablation of the disease lesion; relief, to some extent, of one or more symptoms associated with the disorder; increase in the length of disease-free presentation following treatment, e.g., progression-free survival; increased overall survival; higher response rate; and/or decreased mortality at a given point of time following treatment.

[0101] Administration "in combination with" one or more further therapeutic agents includes concurrent (including simultaneous) and consecutive (i.e., sequential) administration in any order.

[0102] A "pharmaceutically acceptable carrier" refers to a non-toxic solid, semisolid, or liquid filler, diluent, encapsulating material, formulation auxiliary, or carrier conventional in the art for use with a therapeutic agent that together comprise a "pharmaceutical composition" for administration to a subject. A pharmaceutically acceptable carrier is nontoxic to recipients at the dosages and concentrations employed and is compatible with other ingredients of the formulation. The pharmaceutically acceptable carrier is appropriate for the formulation employed. For example, if the therapeutic agent is to be administered orally, the carrier may be a gel capsule. If the therapeutic agent is to be administered subcutaneously, the carrier ideally is not irritable to the skin and does not cause injection site reaction.

Therapeutic Compositions and Methods

Methods of Treating Cancer Having FGFRl Gene Amplifications using FGFRl ECDs and/or FGFRl ECD Fusion Molecules

[0103] In some embodiments, the invention provides methods of treating cancers in which at least a portion of the cancer cells have FGFRl gene amplification. Such cancers have been found, in some embodiments, to be particularly responsive to treatment with a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or FGFRl ECD fusion molecule. Accordingly, in some embodiments, a method of treating cancer having an FGFRl gene amplification comprises administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, a method of treating cancer in a subject comprises administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have an FGFRl gene amplification. In such methods, an FGFRl gene amplification in a cancer is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

[0104] In some embodiments, the invention provides methods of treating cancers in which at least a portion of the cancer cells have overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4. In some embodiments, FGFRl is FGFRlIIIc. In some embodiments, the

overexpression is mRNA overexpression. In some embodiments, the overexpression is protein overexpression. In some embodiments, a method of treating cancer that

overexpresses at least marker selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 comprises administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject. In some embodiments, a method of treating cancer in a subject comprises administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have overexpression of at least marker selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4. In such methods, FGFRl, FGFR3IIIc, FGF2, DKK3, FGF 18, and/or ETV4 overexpression in a cancer is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, FGFRl is FGFRlIIIc.

[0105] In some embodiments, in a cancer with an FGFRl gene amplification, at least a portion of the cancer cells comprise at least four copies of the FGFRl gene. In some embodiments, in a cancer with an FGFRl gene amplification, at least a portion of the cancer cells comprise at least five, at least six, at least 8, or at least 10 copies of the FGFRl gene. Determination of the FGFRl gene copy number can be carried out by any suitable method in the art. Certain nonlimiting exemplary methods are discussed herein. In some embodiments, in a cancer with an FGFRl gene amplification, at least a portion of the cancer cells have a ratio of FGFRl gene to chromosome 8 centromere of at least 2. In some embodiments, in a cancer with an FGFRl gene amplification, at least a portion of the cancer cells have a ratio of FGFRl gene to chromosome 8 centromere of at least 2.5, at least 3, at least 3.5, or at least 4. Determination of such a ratio can be carried out by any suitable method in the art. Certain nonlimiting exemplary methods are discussed herein.

[0106] In some embodiments, the cancer is selected from prostate cancer, breast cancer, colorectal cancer, lung cancer, brain cancer, ovarian cancer, endometrial cancer, head and neck cancer, laryngeal cancer, liver cancer, renal cancer, glioblastoma, and pancreatic cancer. In certain embodiments, the cancer is selected from breast cancer, esophageal cancer, and lung cancer. In some embodiments, the cancer is lung cancer. In some embodiments, the lung cancer is selected from non-small cell lung cancer and small cell lung cancer. In some embodiments, the lung cancer is squamous cell carcinoma. In some embodiments, the cancer is head and neck cancer. In some embodiments, the head and neck cancer is squamous cell carcinoma of the head and neck.

[0107] In some embodiments, the FGFRl ECD has an amino acid sequence selected from SEQ ID NOs: 1 to 4. In some embodiments, the FGFRl ECD has an amino acid sequence selected from SEQ ID NOs: 2 and 4. In some embodiments, the FGFRl ECD fusion molecule has an amino acid sequence selected from SEQ ID NOs: 5 and 6. In some embodiments, the FGFRl ECD fusion molecule is FGFRl ECD.339-Fc with an amino acid sequence of SEQ ID NO: 6.

[0108] In some embodiments, an FGFRl ECD or FGFRl ECD fusion molecule is administered with one or more additional anti-cancer therapies. Examples of the additional anti-cancer therapies include, without limitation, surgery, radiation therapy (radiotherapy), biotherapy, immunotherapy, and chemotherapy or a combination of these therapies. In addition, cytotoxic agents, anti-angiogenic and anti-proliferative agents can be used in combination with the FGFRl ECD or FGFRl ECD fusion molecule. In certain aspects of any of the methods and uses, the invention provides treating cancer in which at least a portion of the cancer cells comprise an FGFRl gene amplification and/or overexpress at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4, by administering therapeutically effective amounts of an FGFRl ECD and/or FGFRl ECD fusion molecule and one or more chemotherapeutic agents to a subject. In some embodiments, the subject's cancer has not previously been treated. A variety of chemotherapeutic agents may be used in the combined treatment methods and uses of the invention. An exemplary and non-limiting list of chemotherapeutic agents contemplated is provided herein under "Definitions" and in the "Summary of the Invention." In some embodiments, the invention provides methods of treating cancer, by administering therapeutically effective amounts of an FGFRl ECD and/or FGFRl ECD fusion molecule and one or more anti-angiogenic agent(s) to a subject. In some embodiments, the invention provides treating cancer, by administering therapeutically effective amounts of an FGFRl ECD and/or FGFRl ECD fusion molecule and one or more VEGF antagonists to a subject. In some embodiments, the invention provides treating cancer, by administering

therapeutically effective amounts of an FGFRl ECD and/or FGFRl ECD fusion molecule and one or more VEGF antagonists in combination with one or more chemotherapeutic agents to a subject. In some embodiments, the one or more VEGF antagonists are anti-VEGF antibodies and/or VEGF traps.

[0109] In some embodiments, methods of treating cancer comprising administering to a subject an FGFRl ECD and/or FGFRl ECD fusion molecule in combination with at least one additional therapeutic agent selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, sorafenib, etoposide, topotecan, a VEGF antagonist, an anti-VEGF antibody, a VEGF trap, and bevacizumab are provided. In another example, methods of treating cancer comprising administering to a subject an FGFR1-ECD.339-Fc in combination with at least one additional therapeutic agent selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, sorafenib, etoposide, topotecan, a VEGF antagonist, an anti-VEGF antibody, a VEGF trap, and bevacizumab are provided. In some embodiments, methods of treating cancer comprising administering to a subject an FGFR1-ECD.339-Fc and docetaxel are provided.

[0110] Pharmaceutical compositions comprising FGFRl ECD and/or FGFRl ECD fusion molecules (e.g., FGFR1-ECD.339-Fc) are administered in a therapeutically effective amount for the specific indication. The therapeutically effective amount is typically dependent on the weight of the subject being treated, his or her physical or health condition, the extensiveness of the condition to be treated, and/or the age of the subject being treated. In general, an FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) is to be administered in an amount in the range of about 50 μg/kg body weight to about 100 mg kg body weight per dose. Optionally, the FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) can be administered in an amount in the range of about 100 μg/kg body weight to about 30 mg/kg body weight per dose. Further optionally, the FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) can be administered in an amount in the range of about 0.5 mg/kg body weight to about 20 mg/kg body weight per dose. In certain embodiments, the FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) is administered at a dose of about 8 mg/kg body weight to about 20 mg/kg body weight. In some embodiments, the FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) is administered at a dose of about 8 mg/kg body weight to about 16 mg/kg body weight (or about 10 mg/kg body weight to about 20 mg/kg body weight when calculated using an extinction coefficient of 1.11 mL/mg*cm). In some embodiments, the FGFRl ECD and/or FGFRl ECD fusion molecule (e.g., FGFR1-ECD.339- Fc) is administered at a dose of about 8 mg/kg body weight, about 10 mg/kg body weight, about 1 1 mg/kg body weight, about 12 mg/kg body weight, about 13 mg/kg body weight, about 14 mg/kg body weight, about 15 mg/kg body weight, about 16 mg/kg body weight, about 17 mg/kg body weight, about 18 mg/kg body weight, about 19 mg/kg body weight, or about 20 mg/kg body weight. In some embodiments, the FGFRl fusion protein is administered at a dose of about 10 mg/kg body weight as calculated using an extinction coefficient of 1.1 1 mL/mg*cm. In other embodiments, the FGFRl fusion protein is administered at a dose of about 20 mg/kg body weight as calculated using an extinction coefficient of 1.1 1 mL/mg*cm. The FGFRl ECD and/or FGFRl ECD fusion molecules may also be administered at ranges from one of the above doses to another. In some

embodiments, dosages may be administered twice a week, weekly, every other week, at a frequency between weekly and every other week, every three weeks, every four weeks, or every month.

[0111] In certain embodiments, dosages of the FGFRl ECD and/or FGFRl ECD fusion molecules can be calculated in two ways depending on the extinction coefficient (EC) used. The extinction coefficient differs depending on whether the glycosylation of the proteins is taken into account. In one embodiment, the extinction coefficient based on the amino acid composition of FGFR1-ECD.339-Fc, for example, is 1.42 mL/mg*cm. In another embodiment, when the carbohydrate portion as well as the amino acid portion of FGFRl - ECD.339-Fc is accounted for, the extinction coefficient is 1.1 1 mL/mg*cm. Calculation of the FGFR1-ECD.339-Fc dose using an EC of 1.1 1 mL/mg*cm increases the calculated dose by 28%, as shown in Table 1. Although the doses calculated using the two extinction coefficients are different, the molar concentrations, or the actual amounts of drug

administered, are identical. Unless otherwise noted, the doses disclosed herein are each calculated using the extinction coefficient that does not take account of glycosylation. How these dosages compare to those calculated using the extinction coefficient that takes account of glycosylation for FGFR1-ECD.339-Fc is shown in Table 1. As can be seen from Table 1, a dosage of about 8 mg/kg (e.g., 7.8 and 8.0) using an EC of 1.42 mL/mg*cm herein corresponds to a dosage of about 10 mg/kg (e.g. 10.0 and 10.2) when calculated using an EC of 1.1 1 mL/mg*cm. A dosage of about 16 mg/kg (e.g. 15.6 and 16.0 mg/kg) using an EC of 1.42 mL/mg*cm herein corresponds to a dosage of about 20 mg/kg (e.g. 20.0 and 20.5) when calculated using an EC of 1.11 mL/mg*cm. As noted in the "Definitions" section above, measured numbers provided herein are approximate and encompass values having additional significant digits that are rounded off. For instance, 8 mg/kg encompasses values with two significant digits such as 7.6, 7.8, 8.0, 8.2, 8.4, and 8.45, each of which round to 8. Likewise, a value such as 16 mg/kg encompasses values with three significant digits that round to 16, such as, for example 15.6 and 16.0.

Table 1. Conversion of FGFR1-ECD.339-FC Dose

a Doses shown in mg/kg.

[0112] The pharmaceutical compositions comprising FGFR1 ECDs, FGFR1 ECD fusion molecules, and/or at least one additional therapeutic agent can be administered as needed to subjects. In certain embodiments, an effective dose of a therapeutic molecule is administered to a subject one or more times. In various embodiments, an effective dose of a therapeutic molecule is administered to the subject at least once every two months, at least once a month, at least twice a month, once a week, twice a week, or three times a week. In various embodiments, an effective dose of a therapeutic molecule is administered to the subject for at least a week, at least a month, at least three months, at least six months, or at least a year.

[0113] In certain embodiments, the combined administration of an FGFR1 ECDs, FGFR1 ECD fusion molecule and at least one additional therapeutic agent includes concurrent administration, including simultaneous administration, using separate formulations or a single pharmaceutical formulation, as well as consecutive administration in any order. Optionally there is a time period while both (or all) active agents simultaneously exert their biological activities. Therapeutically effective amounts of therapeutic agents administered in combination with the FGFR1 ECD and/or FGFR1 ECD fusion molecule (e.g., FGFR1- ECD.339-Fc) will be at the physician's or veterinarian's discretion. Dosage administration and adjustment is done to achieve maximal management of the conditions to be treated. The dose will additionally depend on such factors as the type of therapeutic agent to be used, the specific patient being treated, the stage of the disease, and the desired aggressiveness of the treatment regime.

[0114] In certain embodiments, a patient is treated with a combination of the FGFR1 ECD and/or FGFR1 ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) and a VEGF antagonist. In some embodiments, the VEGF antagonist is a VEGF trap (e.g., aflibercept). In some embodiments, the VEGF antagonist is a tyrosine kinase inhibitor (e.g., pazopanib). In some embodiments, the VEGF antagonist is an anti-VEGF antibody. In some embodiments, the VEGF antibody is bevacizumab. One exemplary dosage of bevacizumab is in the range from about 0.05 mg/kg to about 20 mg/kg. Thus, one or more doses of about 0.5 mg/kg, 2.0 mg/kg, 4.0 mg/kg, 7.5 mg/kg, 10 mg/kg or 15 mg/kg (or any combination thereof) may be administered to the patient. Such doses may be administered intermittently, e.g., every week, every two, or every three weeks.

[0115] In some embodiments, the FGFR1 ECD and/or FGFR1 ECD fusion molecule (e.g., FGFR1-ECD.339-Fc) is administered in combination with another therapeutic agent, such as chemotherapeutic agent or anti-angiogenic agent, at the recommended or prescribed dosage and/or frequency of the therapeutic agent.

[0116] In some embodiments, an additional therapeutic agent is administered at a dosage approved by an agency responsible for approving therapeutic treatments, such as the Food and Drug Administration, or at the manfacturer's recommended dosage. Routes of Administration and Carriers

[0117] In some embodiments, an FGFR1 ECD and/or FGFR1 ECD fusion molecule can be administered intravenously and/or subcutaneously. In some embodiments, an FGFR1 ECD and/or FGFR1 ECD fusion molecule can be administered by another route, such as intra-arterial, parenteral, intranasal, intramuscular, intracardiac, intraventricular, intratracheal, buccal, rectal, intraperitoneal, intradermal, topical, transdermal, or intrathecal, or otherwise by implantation or inhalation. In various embodiments, at least one additional therapeutic agent can be administered in vivo by a variety of routes, including intravenous, intra-arterial, subcutaneous, parenteral, intranasal, intramuscular, intracardiac, intraventricular, intratracheal, buccal, rectal, intraperitoneal, intradermal, topical, transdermal, and intrathecal, or otherwise by implantation or inhalation. Each of the subject compositions can be formulated alone or in combination into preparations in solid, semi-solid, liquid, or gaseous forms, such as tablets, capsules, powders, granules, ointments, solutions, suppositories, enemas, injections, inhalants, and aerosols.

[0118] In various embodiments, compositions comprising an FGFR1 ECD, FGFR1 ECD fusion molecule, and/or at least one additional therapeutic agent are provided in formulation with pharmaceutically acceptable carriers, a wide variety of which are known in the art (see, e.g., Gennaro, Remington: The Science and Practice of Pharmacy with Facts and

Comparisons: Drugfacts Pius, 20th ed. (2003); Ansel et al, Pharmaceutical Dosage Forms and Drug Delivery Systems, 7^th ed., Lippencott Williams and Wilkins (2004); Kibbe et al, Handbook of Pharmaceutical Excipients, 3^rd ed., Pharmaceutical Press (2000)). Various pharmaceutically acceptable carriers, which include vehicles, adjuvants, carriers, and diluents, are available to the public. Moreover, various pharmaceutically acceptable auxiliary substances, such as pH adjusting and buffering agents, tonicity adjusting agents, stabilizers, wetting agents and the like, are also available to the public. Certain non-limiting exemplary carriers include saline, buffered saline, dextrose, water, glycerol, ethanol, and combinations thereof. In some embodiments, a therapeutic agent is formulated as the brand-name drug indicated above in the Definitions section, or a generic equivalent. In some embodiments, docetaxel is formulated as Taxotere® (Sanofi Aventis) or a generic equivalent.

[0119] In various embodiments, compositions comprising FGFR1 ECDs, FGFR1 ECD fusion molecules, and/or at least one additional therapeutic agent can be formulated for injection by dissolving, suspending, or emulsifying them in an aqueous or nonaqueous solvent, such as vegetable or other oils, synthetic aliphatic acid glycerides, esters of higher aliphatic acids, or propylene glycol; and if desired, with conventional additives such as solubilizers, isotonic agents, suspending agents, emulsifying agents, stabilizers and preservatives. In various embodiments, the compositions may be formulated for inhalation, for example, using pressurized acceptable propellants such as dichlorodifluoromethane, propane, nitrogen, and the like. The compositions may also be formulated, in various embodiments, into sustained release microcapsules, such as with biodegradable or nonbiodegradable polymers. A non-limiting exemplary biodegradable formulation includes poly lactic acid-glycolic acid polymer. A non-limiting exemplary non-biodegradable formulation includes a polyglycerin fatty acid ester. Certain methods of making such formulations are described, for example, in EP 1 125 584 Al .

[0120] Pharmaceutical dosage packs comprising one or more containers, each containing one or more doses of an FGFRl ECD, an FGFRl ECD fusion molecule, and/or at least one additional therapeutic agent are also provided. In certain embodiments, a unit dosage is provided wherein the unit dosage contains a predetermined amount of a composition comprising an FGFRl ECD, an FGFRl ECD fusion molecule, and/or at least one additional therapeutic agent with or without one or more additional agents. In certain embodiments, such a unit dosage is supplied in single-use prefilled syringe for injection. In various embodiments, the composition contained in the unit dosage may comprise saline, sucrose, or the like; a buffer, such as phosphate, or the like; and/or be formulated within a stable and effective pH range. Alternatively, in certain embodiments, the composition may be provided as a lyophilized powder that can be reconstituted upon addition of an appropriate liquid, for example, sterile water. In certain embodiments, a composition comprises one or more substances that inhibit protein aggregation, including, but not limited to, sucrose and arginine. In certain embodiments, a composition of the invention comprises heparin and/or a proteoglycan.

[0121] In some embodiments, a dosage pack comprises instructions to determine whether a cancer comprises an FGFRl gene amplification and/or overexpresses at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 prior to administering an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, FGFRl is FGFRl IIIc. In some such embodiments, the instructions indicate that the presence of a FGFRl gene amplification and/or overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIC, FGF2, DKK3, FGF18, and ETV4 in at least a portion of the cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that the presence of at least four copies of an FGFRl gene in at least a portion of the cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that the presence of at least four, at least six, at least eight, or at least ten copies of an FGFRl gene in at least a portion of the cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that a ratio of FGFRl gene to chromosome 8 centromere of at least 2 in at least a portion of the cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that a ratio of FGFRl gene to chromosome 8 centromere of at least 2.5, at least 3, at least 3.5, or at least 4 in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule.

[0122] In some embodiments, a dosage pack comprises instructions to determine whether a lung cancer comprises an FGFRl gene amplification and/or overexpresses at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 prior to administering an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, FGFRl is FGFRlIIIc. In some such embodiments, the instructions indicate that the presence of an FGFRl gene amplification and/or overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that the presence of at least four copies of an FGFRl gene in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that the presence of at least four, at least six, at least eight, or at least ten copies of an FGFRl gene in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that a ratio of FGFRl gene to chromosome 8 centromere of at least 2 in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. In some embodiments, the instructions indicate that a ratio of FGFRl gene to chromosome 8 centromere of at least 2.5, at least 3, at least 3.5, or at least 4 in at least a portion of the lung cancer cells is indicative of therapeutic responsiveness to an FGFRl ECD and/or an FGFRl ECD fusion molecule. [0123] The term "instructions," as used herein includes, but is not limited to, labels, package inserts, instructions available in electronic form such as on a computer readable medium (e.g., a diskette, compact disk, or DVD), instructions available remotely such as over the internet, etc. A dosage pack is considered to include the instructions when the dosage pack provides access to the instructions, a link to the instructions (such as a uniform resource locator, or url), or other mechanism for obtaining a copy of the instructions (such as a return reply card, a physical address from which instructions may be requested, an e-mail address from which instructions may be requested, a phone number that may be called to obtain instructions, etc.).

FGFRl ECDs and FGFRl ECD fusion molecules

[0124] Nonlimiting exemplary FGFRl ECDs include full-length FGFRl ECDs, FGFRl ECD fragments, and FGFRl ECD variants. FGFRl ECDs may include or lack a signal peptide. Exemplary FGFRl ECDs include, but are not limited to, FGFRl ECDs having amino acid sequences selected from SEQ ID NOs.: 1, 2, 3, and 4.

[0125] Non-limiting exemplary FGFRl ECD fragments include human FGFRl ECD ending at amino acid 339 (counting from the first amino acid of the mature form, without the signal peptide). In some embodiments, an FGFRl ECD fragment ends at an amino acid between amino acid 339 and amino acid 360 (counting from the first amino acid of the mature form, without the signal peptide). Exemplary FGFRl ECD fragments include, but are not limited to, FGFRl ECD fragments having amino acid sequences selected from SEQ ID NOs.: 3 and 4.

[0126] In some embodiments, an FGFRl ECD comprises a sequence selected from SEQ ID NOs: 1 to 4. In some embodiments, an FGFRl ECD consists of a sequence selected from SEQ ID NOs: 1 to 4. When an FGFRl ECD "consists of a sequence selected from SEQ ID NOs: 1 to 4, the FGFRl ECD may or may not contain various post-translational

modifications, such as glycosylation and sialylation. In other words, when an FGFRl ECD consists of a particular amino acid sequence, it does not contain additional amino acids in the contiguous amino acid sequence, but may contain modifications to amino acid side chains, the N-terminal amino group, and/or the C-terminal carboxy group.

[0127] In some embodiments, an FGFRl ECD fusion molecule comprises a signal peptide. In some embodiments, an FGFRl ECD fusion molecule lacks a signal peptide. In some embodiments, the FGFRl ECD portion of an FGFRl ECD fusion molecule comprises a sequence selected from SEQ ID NOs: 1 to 4. In some embodiments, the FGFRl ECD portion of an FGFRl ECD fusion molecule consists of a sequence selected from SEQ ID NOs: 1 to 4. When an FGFRl ECD portion of an FGFRl ECD fusion molecule "consists of a sequence selected from SEQ ID NOs: 1 to 4, the FGFRl ECD portion of an FGFRl ECD fusion molecule may or may not contain various post-translational modifications, such as glycosylation and sialylation. In other words, when an FGFRl ECD portion of an FGFRl ECD fusion molecule consists of a particular amino acid sequence, it does not contain additional amino acids from FGFRl in the contiguous amino acid sequence, but may contain modifications to amino acid side chains, the N-terminal amino group, and/or the C-terminal carboxy group. Further, because the FGFRl ECD is linked to a fusion molecule, there may be additional amino acids at the N- and/or C-terminus of the FGFRl ECD, but those amino acids are not from the FGFRl sequence, but may be from, for example, a linker sequence, or a fusion partner sequence.

[0128] In some embodiments, the fusion partner portion of an FGFRl ECD fusion molecule is selected from Fc, albumin, and polyethylene glycol. Nonlimiting exemplary fusion partners are discussed herein.

[0129] The inventors have found that administration of an FGFRl ECD and/or an FGFRl ECD fusion molecule and at least one additional therapeutic agent selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, sorafenib, etoposide, topotecan, a VEGF antagonist, pazopanib, an anti-VEGF antibody, a VEGF trap, and bevacizumab is useful for treating cancers in which at least a portion of the cancer cells have FGFRl gene amplification and/or overexpress at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4. In some embodiments, FGFRl is FGFRlIIIc. In some embodiments, an FGFRl ECD and/or an FGFRl ECD fusion molecule is administered with docetaxel.

Fusion Partners and Conjugates

[0130] As discussed herein, an FGFRl ECD may be combined with at least one fusion partner, resulting in an FGFRl ECD fusion molecule. These fusion partners may facilitate purification, and the FGFRl ECD fusion molecules may show an increased half-life in vivo. Suitable fusion partners of an FGFRl ECD include, for example, polymers, such as water soluble polymers, the constant domain of immunoglobulins; all or part of human serum albumin (HSA); fetuin A; fetuin B; a leucine zipper domain; a tetranectin trimerization domain; mannose binding protein (also known as mannose binding lectin), for example, mannose binding protein 1 ; and an Fc region, as described herein and further described in U.S. Patent No. 6,686, 179. Nonlimiting exemplary FGFR1 ECD fusion molecules are described, e.g., in U.S. Patent No. 7,678,890.

[0131] An FGFR1 ECD fusion molecule may be prepared by attaching polyaminoacids or branch point amino acids to the FGFR1 ECD. For example, the polyaminoacid may be a carrier protein that serves to increase the circulation half life of the FGFR1 ECD (in addition to the advantages achieved via a fusion molecule). For the therapeutic purpose of the present invention, such polyaminoacids should ideally be those that have or do not create neutralizing antigenic responses, or other adverse responses. Such polyaminoacids may be chosen from serum albumin (such as HSA), an additional antibody or portion thereof, for example the Fc region, fetuin A, fetuin B, leucine zipper nuclear factor erythroid derivative-2 (NFE2), neuroretinal leucine zipper, tetranectin, or other polyaminoacids, for example, lysines. As described herein, the location of attachment of the polyaminoacid may be at the N terminus or C terminus, or other places in between, and also may be connected by a chemical linker moiety to the selected molecule.

Polymers

[0132] Polymers, for example, water soluble polymers, may be useful as fusion partners to reduce precipitation of the FGFR1 ECD fusion molecule in an aqueous environment, such as typically found in a physiological environment. Polymers employed in the invention will be pharmaceutically acceptable for the preparation of a therapeutic product or composition.

[0133] Suitable, clinically acceptable, water soluble polymers include, but are not limited to, polyethylene glycol (PEG), polyethylene glycol propionaldehyde, copolymers of ethylene glycol/propylene glycol, monomethoxy-polyethylene glycol, carboxymethylcellulose, dextran, polyvinyl alcohol (PVA), polyvinyl pyrrolidone, poly-l,3-dioxolane, poly-1,3,6- trioxane, ethylene/maleic anhydride copolymer, poly (β-amino acids) (either homopolymers or random copolymers), poly(n-vinyl pyrrolidone) polyethylene glycol, polypropylene glycol homopolymers (PPG) and other polyakylene oxides, polypropylene oxide/ethylene oxide copolymers, polyoxyethylated polyols (POG) (e.g., glycerol) and other polyoxyethylated polyols, polyoxyethylated sorbitol, or polyoxyethylated glucose, colonic acids or other carbohydrate polymers, Ficoll, or dextran and mixtures thereof.

[0134] As used herein, polyethylene glycol (PEG) is meant to encompass any of the forms that have been used to derivatize other proteins, such as mono-(Ci-Cio) alkoxy- or aryloxy- polyethylene glycol. Polyethylene glycol propionaldehyde may have advantages in manufacturing due to its stability in water. [0135] Polymers used herein, for example water soluble polymers, may be of any molecular weight and may be branched or unbranched. In some embodiments, the polymers have an average molecular weight of between about 2 kDa to about 100 kDa (the term "about" indicating that in preparations of a polymer, some molecules will weigh more, some less, than the stated molecular weight). The average molecular weight of each polymer may be between about 5 kDa and about 50 kDa, or between about 12 kDa and about 25 kDa. Generally, the higher the molecular weight or the more branches, the higher the

polymenprotein ratio. Other sizes may also be used, depending on the desired therapeutic profile; for example, the duration of sustained release; the effects, if any, on biological activity; the ease in handling; the degree or lack of antigenicity; and other known effects of a polymer on an FGFR1 ECD.

[0136] Polymers employed in the present invention are typically attached to an FGFR1 ECD with consideration of effects on functional or antigenic domains of the polypeptide. In general, chemical derivatization may be performed under any suitable condition used to react a protein with an activated polymer molecule. Activating groups which can be used to link the polymer to the active moieties include sulfone, maleimide, sulfhydryl, thiol, triflate, tresylate, azidirine, oxirane, and 5-pyridyl.

[0137] Polymers of the invention are typically attached to a heterologous polypeptide at the alpha (a) or epsilon (ε) amino groups of amino acids or a reactive thiol group, but it is also contemplated that a polymer group could be attached to any reactive group of the protein that is sufficiently reactive to become attached to a polymer group under suitable reaction conditions. Thus, a polymer may be covalently bound to an FGFR1 ECD via a reactive group, such as a free amino or carboxyl group. The amino acid residues having a free amino group may include lysine residues and the N-terminal amino acid residue. Those having a free carboxyl group may include aspartic acid residues, glutamic acid residues, and the C- terminal amino acid residue. Those having a reactive thiol group include cysteine residues.

[0138] Methods for preparing fusion molecules conjugated with polymers, such as water soluble polymers, will each generally involve (a) reacting an FGFR1 ECD with a polymer under conditions whereby the polypeptide becomes attached to one or more polymers and (b) obtaining the reaction product. Reaction conditions for each conjugation may be selected from any of those known in the art or those subsequently developed, but should be selected to avoid or limit exposure to reaction conditions such as temperatures, solvents, and pH levels that would inactivate the protein to be modified. In general, the optimal reaction conditions for the reactions will be determined case-by -case based on known parameters and the desired result. For example, the larger the ratio of polymenpolypeptide conjugate, the greater the percentage of conjugated product. The optimum ratio (in terms of efficiency of reaction in that there is no excess unreacted polypeptide or polymer) may be determined by factors such as the desired degree of derivatization (e.g., mono-, di-, tri-, etc.), the molecular weight of the polymer selected, whether the polymer is branched or unbranched and the reaction conditions used. The ratio of polymer (for example, PEG) to a polypeptide will generally range from 1 : 1 to 100 : 1. One or more purified conjugates may be prepared from each mixture by standard purification techniques, including among others, dialysis, salting-out, ultrafiltration, ion-exchange chromatography, gel filtration chromatography, and electrophoresis.

[0139] One may specifically desire an N-terminal chemically modified FGFR1 ECD. One may select a polymer by molecular weight, branching, etc., the proportion of polymers to FGFR1 ECD molecules in the reaction mix, the type of reaction to be performed, and the method of obtaining the selected N-terminal chemically modified FGFR1 ECD. The method of obtaining the N-terminal chemically modified FGFR1 ECD preparation (separating this moiety from other monoderivatized moieties if necessary) may be by purification of the N- terminal chemically modified FGFR1 ECD material from a population of chemically modified protein molecules.

[0140] Selective N-terminal chemical modification may be accomplished by reductive alkylation which exploits differential reactivity of different types of primary amino groups (lysine versus the N-terminal) available for derivatization in a particular protein. Under the appropriate reaction conditions, substantially selective derivatization of the protein at the N terminus with a carbonyl group-containing polymer is achieved. For example, one may selectively attach a polymer to the N terminus of the protein by performing the reaction at a pH that allows one to take advantage of the pKa differences between the ε-amino group of the lysine residues and that of the a-amino group of the N-terminal residue of the protein. By such selective derivatization, attachment of a polymer to a protein is controlled: the conjugation with the polymer takes place predominantly at the N terminus of the protein and no significant modification of other reactive groups, such as the lysine side chain amino groups, occurs. Using reductive alkylation, the polymer may be of the type described above and should have a single reactive aldehyde for coupling to the protein. Polyethylene glycol propionaldehyde, containing a single reactive aldehyde, may also be used.

[0141] In one embodiment, the present invention contemplates the chemically derivatized FGFR1 ECD to include mono- or poly- (e.g., 2-4) PEG moieties. Pegylation may be carried out by any of the pegylation reactions available. Methods for preparing a pegylated protein product will generally include (a) reacting a polypeptide with polyethylene glycol (such as a reactive ester or aldehyde derivative of PEG) under conditions whereby the protein becomes attached to one or more PEG groups; and (b) obtaining the reaction product(s). In general, the optimal reaction conditions will be determined case by case based on known parameters and the desired result.

[0142] There are a number of PEG attachment methods known in the art. See, for example, EP 0 401 384; Malik et al, Exp. Hematol, 20: 1028-1035 (1992); Francis, Focus on Growth Factors, 3(2):4-10 (1992); EP 0 154 316; EP 0 401 384; WO 92/16221; WO

95/34326; and the other publications cited herein that relate to pegylation.

[0143] Pegylation may be carried out, e.g., via an acylation reaction or an alkylation reaction with a reactive polyethylene glycol molecule. Thus, protein products according to the present invention include pegylated proteins wherein the PEG group(s) is (are) attached via acyl or alkyl groups. Such products may be mono-pegylated or poly-pegylated (for example, those containing 2-6 or 2-5 PEG groups). The PEG groups are generally attached to the protein at the a- or ε-amino groups of amino acids, but it is also contemplated that the PEG groups could be attached to any amino group attached to the protein that is sufficiently reactive to become attached to a PEG group under suitable reaction conditions.

[0144] Pegylation by acylation generally involves reacting an active ester derivative of polyethylene glycol (PEG) with an FGFR1 ECD. For acylation reactions, the polymer(s) selected typically have a single reactive ester group. Any known or subsequently discovered reactive PEG molecule may be used to carry out the pegylation reaction. An example of a suitable activated PEG ester is PEG esterified to N-hydroxysuccinimide (NHS). As used herein, acylation is contemplated to include, without limitation, the following types of linkages between the therapeutic protein and a polymer such as PEG: amide, carbamate, urethane, and the like, see for example, Chamow, Bioconjugate Chem., 5: 133-140 (1994). Reaction conditions may be selected from any of those currently known or those

subsequently developed, but should avoid conditions such as temperature, solvent, and pH that would inactivate the polypeptide to be modified.

[0145] Pegylation by acylation will generally result in a poly-pegylated protein. The connecting linkage may be an amide. The resulting product may be substantially only (e.g., > 95%) mono-, di-, or tri-pegylated. However, some species with higher degrees of pegylation may be formed in amounts depending on the specific reaction conditions used. If desired, more purified pegylated species may be separated from the mixture (particularly unreacted species) by standard purification techniques, including among others, dialysis, salting-out, ultrafiltration, ion-exchange chromatography, gel filtration chromatography, and

electrophoresis.

[0146] Pegylation by alkylation generally involves reacting a terminal aldehyde derivative of PEG with a polypeptide in the presence of a reducing agent. For the reductive alkylation reaction, the polymer(s) selected should have a single reactive aldehyde group. An exemplary reactive PEG aldehyde is polyethylene glycol propionaldehyde, which is water stable, or mono Ci-Cio alkoxy or aryloxy derivatives thereof, see for example, U.S. Pat. No. 5,252,714.

Markers

[0147] Moreover, FGFR1 ECDs of the present invention may be fused to marker sequences, such as a peptide that facilitates purification of the fused polypeptide. The marker amino acid sequence may be a hexa-histidine peptide such as the tag provided in a pQE vector (Qiagen, Mississauga, Ontario, Canada), among others, many of which are

commercially available. As described in Gentz et al, Proc. Natl. Acad. Sci. 86:821-824 (1989), for instance, hexa-histidine provides for convenient purification of the fusion protein. Another peptide tag useful for purification, the hemagglutinin (HA) tag, corresponds to an epitope derived from the influenza HA protein. (Wilson et al, Cell 37:767 (1984)). Any of these above fusions may be engineered using the FGFR1 ECDs described herein.

Oligomerization Domain Fusion Partners

[0148] In various embodiments, oligomerization offers some functional advantages to a fusion protein, including, but not limited to, multivalency, increased binding strength, and the combined function of different domains. Accordingly, in some embodiments, a fusion partner comprises an oligomerization domain, for example, a dimerization domain.

Exemplary oligomerization domains include, but are not limited to, coiled-coil domains, including alpha-helical coiled-coil domains; collagen domains; collagen-like domains; and certain immunoglobulin domains. Exemplary coiled-coil polypeptide fusion partners include, but are not limited to, the tetranectin coiled-coil domain; the coiled-coil domain of cartilage oligomeric matrix protein; angiopoietin coiled-coil domains; and leucine zipper domains. Exemplary collagen or collagen-like oligomerization domains include, but are not limited to, those found in collagens, mannose binding lectin, lung surfactant proteins A and D, adiponectin, ficolin, conglutinin, macrophage scavenger receptor, and emilin.

Antibody Fc Immunoglobulin Domain Fusion Partners

[0149] Many Fc domains that may be used as fusion partners are known in the art. In some embodiments, a fusion partner is an Fc immunoglobulin domain. An Fc fusion partner may be a wild-type Fc found in a naturally occurring antibody, a variant thereof, or a fragment thereof. Non-limiting exemplary Fc fusion partners include Fes comprising a hinge and the CH2 and CH3 constant domains of a human IgG, for example, human IgGl, IgG2, IgG3, or IgG4. Additional exemplary Fc fusion partners include, but are not limited to, human IgA and IgM. In some embodiments, an Fc fusion partner comprises a C237S mutation, for example, in an IgGl (see, for example, SEQ ID NO: 8). In some embodiments, an Fc fusion partner comprises a hinge, CH2, and CH3 domains of human IgG2 with a P33 IS mutation, as described in U.S. Patent No. 6,900,292. Certain exemplary Fc domain fusion partners are shown in SEQ ID NOs: 8 to 10.

Albumin Fusion Partners and Albumin-binding Molecule Fusion Partners

[0150] In some embodiments, a fusion partner is an albumin. Exemplary albumins include, but are not limited to, human serum album (HSA) and fragments of HSA that are capable of increasing the serum half-life or bioavailability of the polypeptide to which they are fused. In some embodiments, a fusion partner is an albumin-binding molecule, such as, for example, a peptide that binds albumin or a molecule that conjugates with a lipid or other molecule that binds albumin. In some embodiments, a fusion molecule comprising HSA is prepared as described, e.g., in U.S. Patent No. 6,686, 179.

Exemplary Attachment of Fusion Partners

[0151] The fusion partner may be attached, either covalently or non-covalently, to the N terminus or the C terminus of the FGFRl ECD. The attachment may also occur at a location within the FGFRl ECD other than the N terminus or the C terminus, for example, through an amino acid side chain (such as, for example, the side chain of cysteine, lysine, serine, or threonine).

[0152] In either covalent or non-covalent attachment embodiments, a linker may be included between the fusion partner and the FGFRl ECD. Such linkers may be comprised of at least one amino acid or chemical moiety. Exemplary methods of covalently attaching a fusion partner to an FGFRl ECD include, but are not limited to, translation of the fusion partner and the FGFRl ECD as a single amino acid sequence and chemical attachment of the fusion partner to the FGFRl ECD. When the fusion partner and an FGFRl ECD are translated as single amino acid sequence, additional amino acids may be included between the fusion partner and the FGFRl ECD as a linker. In some embodiments, the linker is selected based on the polynucleotide sequence that encodes it, to facilitate cloning the fusion partner and/or FGFRl ECD into a single expression construct (for example, a polynucleotide containing a particular restriction site may be placed between the polynucleotide encoding the fusion partner and the polynucleotide encoding the FGFRl ECD, wherein the polynucleotide containing the restriction site encodes a short amino acid linker sequence). When the fusion partner and the FGFRl ECD are covalently coupled by chemical means, linkers of various sizes may typically be included during the coupling reaction.

[0153] Exemplary methods of non-covalently attaching a fusion partner to an FGFRl ECD include, but are not limited to, attachment through a binding pair. Exemplary binding pairs include, but are not limited to, biotin and avidin or streptavidin, an antibody and its antigen, etc.

Co-Translational and Post-Translational Modifications

[0154] The invention encompasses administration of FGFRl ECDs and FGFRl ECD fusion molecules that are differentially modified during or after translation, for example by glycosylation, acetylation, phosphorylation, amidation, derivatization by known

protecting/blocking groups, proteolytic cleavage, or linkage to an antibody molecule or other cellular ligand. Any of numerous chemical modifications may be carried out by known techniques, including, but not limited to, specific chemical cleavage by cyanogen bromide, trypsin, chymotrypsin, papain, V8 protease; NABH_4; acetylation; formylation; oxidation; reduction; and/or metabolic synthesis in the presence of tunicamycin.

[0155] Additional post-translational modifications encompassed by the invention include, for example, for example, N-linked or O-linked carbohydrate chains, processing of N- terminal or C-terminal ends), attachment of chemical moieties to the amino acid backbone, chemical modifications of N-linked or O-linked carbohydrate chains, and addition or deletion of an N-terminal methionine residue as a result of prokaryotic host cell expression. A nonlimiting discussion of various post-translational modifications of FGFRl ECDs and

FGFRl ECD fusion molecules can be found, e.g., in U.S. Patent No. 7,678,890.

FGFRl ECD and FGFRl ECD Fusion Molecule Expression and Production Vectors

[0156] Vectors comprising polynucleotides that encode FGFRl ECDs are provided. Vectors comprising polynucleotides that encode FGFRl ECD fusion molecules are also provided. Such vectors include, but are not limited to, DNA vectors, phage vectors, viral vectors, retroviral vectors, etc.

[0157] In some embodiments, a vector is selected that is optimized for expression of polypeptides in CHO or CHO-derived cells. Exemplary such vectors are described, e.g., in Running Deer et al., Biotechnol. Prog. 20:880-889 (2004). [0158] In some embodiments, a vector is chosen for in vivo expression of FGFRl ECDs and/or FGFRl ECD fusion molecules in animals, including humans. In some such embodiments, expression of the polypeptide is under the control of a promoter that functions in a tissue-specific manner. For example, liver-specific promoters are described, e.g., in PCT Publication No. WO 2006/076288. A nonlimiting discussion of various expression vectors can be found, e.g., in U.S. Patent No. 7,678,890.

Host Cells

[0159] In various embodiments, FGFRl ECDs or FGFRl ECD fusion molecules may be expressed in prokaryotic cells, such as bacterial cells; or in eukaryotic cells, such as fungal cells, plant cells, insect cells, and mammalian cells. Such expression may be carried out, for example, according to procedures known in the art. Exemplary eukaryotic cells that may be used to express polypeptides include, but are not limited to, COS cells, including COS 7 cells; 293 cells, including 293 -6E cells; CHO cells, including CHO-S and DG44 cells; and NSO cells. In some embodiments, a particular eukaryotic host cell is selected based on its ability to make certain desired post-translational modifications to the FGFRl ECDs or FGFRl ECD fusion molecules. For example, in some embodiments, CHO cells produce FGFRl ECDs and/or FGFRl ECD fusion molecules that have a higher level of sialylation than the same polypeptide produced in 293 cells.

[0160] Introduction of a nucleic acid into a desired host cell may be accomplished by any method known in the art, including but not limited to, calcium phosphate transfection, DEAE-dextran mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection, etc. Nonlimiting exemplary methods are described, e.g., in Sambrook et al, Molecular Cloning, A Laboratory Manual, 3^rd ed. Cold Spring Harbor Laboratory Press (2001). Nucleic acids may be transiently or stably transfected in the desired host cells, according to methods known in the art. A nonlimiting discussion of host cells and methods of polypeptides in host cells can be found, e.g., in U.S. Patent No. 7,678,890.

[0161] In some embodiments, a polypeptide may be produced in vivo in an animal that has been engineered or transfected with a nucleic acid molecule encoding the polypeptide, according to methods known in the art.

Purification of FGFRl ECD Polypeptides

[0162] FGFRl ECDs or FGFRl ECD fusion molecules may be purified by various methods known in the art. Such methods include, but are not limited to, the use of affinity matrices or hydrophobic interaction chromatography. Suitable affinity ligands include any ligands of the FGFRl ECD or of the fusion partner. Suitable affinity ligands in the case of an antibody that binds FGFRl include, but are not limited to, FGFRl itself and fragments thereof. Further, a Protein A, Protein G, Protein A/G, or an antibody affinity column may be used to bind to an Fc fusion partner to purify an FGFRl ECD fusion molecule. Antibodies to FGFRl ECD may also be used to purify FGFRl ECD or FGFRl ECD fusion molecules. Hydrophobic interactive chromatography, for example, a butyl or phenyl column, may also suitable for purifying some polypeptides. Many methods of purifying polypeptides are known in the art. A nonlimiting discussion of various methods of purifying polypepides can be found, e.g., in U.S. Patent No. 7,678,890.

Methods of Identifying Patients Who Would Benefit from FGFRl ECDs and/or FGFRl ECD Fusion Molecules

[0163] In some embodiments, methods of identifying patients with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule are provided. In some such embodiments, the method comprises determining whether at least a portion of the cancer cells comprise an FGFRl gene amplification in a sample obtained from the subject. In some embodiments, FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, a sample is taken from a patient having or suspected of having cancer. A finding of FGFRl gene amplification in at least a portion of the cancer cells indicates that the patient having or suspected of having cancer may benefit from an FGFRl ECD or FGFRl ECD fusion molecule therapy. In some embodiments, the patient has or is suspected of having lung cancer.

[0164] In some embodiments, the method comprises determining whether at least a portion of the cancer cells comprise overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF 18, and ETV4 in a sample obtained from the subject. In some embodiments, the overexpression is mRNA overexpression. In some embodiments, the overexpression is protein overexpression. In some embodiments, FGFRl, FGFR3IIIc, FGF2, DKK3, FGF 18, and/or ETV4 overexpression is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule. In some embodiments, a sample is taken from a patient having or suspected of having cancer. A finding of FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and/or ETV4 overexpression in at least a portion of the cancer cells indicates that the patient having or suspected of having cancer may benefit from an FGFRl ECD or FGFRl ECD fusion molecule therapy. In some embodiments, FGFRl is FGFRl IIIc. In some embodiments, the patient has or is suspected of having lung cancer. [0165] In some embodiments, FGFRl gene amplification and/or overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 is determined by a laboratory. A laboratory may be a hospital laboratory or a laboratory independent of a hospital. In some embodiments, following a determination of FGFRl gene amplification and/or overexpression of at least one, at least two, at least three, or at least four markers selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4, the results of the determination are communicated to a medical professional. In some such embodiments, the results are communicated for the purpose of determining whether a patient should benefit from, or be responsive to, an FGFRl ECD or FGFRl ECD fusion molecule therapy. In some embodiments, medical professionals include, but are not limited to, doctors, nurses, hospital administration and staff, etc. In some embodiments, FGFRl is FGFRlIIIc.

[0166] Any suitable method of determining FGFRl gene amplification may be used. Nonlimiting exemplary such methods include fluorescence in situ hybridization (FISH; see, e.g., Monni et al. (2001) PNAS 98: 5711-5716), array comparative genomic hybridization (aCGH), DNA microarrays (see, e.g., Carter et al. (2007) Nat. Genet. 39: S16-21), spectral karyotyping (SKY; see, e.g. Liyanage et al. (1996) Nat. Genet. 14: 312-5), real-time quantitative PCR (see, e.g., Dhaene et al. (2010) Methods 50: 262-270), southern blotting, and sequencing, including, but not limited to, high-throughput sequencing (HTS; see, e.g. Medvedev et al. (2010) Genome Res. 20: 1613-22), and next generation sequencing technologies such as RNA-seq, also called "Whole Transcriptome Shotgun Sequencing" ("WTSS"), Applied Biosystems SOLiD™ System, Illumina (Solexa) sequencing, Ion semiconductor sequencing, DNA nanoball sequencing, Helioscope(TM) single molecule sequencing, Single Molecule SMRT(TM) sequencing, Single Molecule real time (RNAP) sequencing, Nanopore DNA sequencing, VisiGen Biotechnologies approach, and 454 pyrosequencing.

[0167] Fluorescence in situ hybridization (FISH) is a cytogenetic technique to detect and localize the presence or absence of specific DNA sequences on chromosomes. In some embodiments, FISH uses fluorescent probes to detect certain regions of chromosomes in a sequence-specific manner. Thus, in some embodiments, to detect gene amplification in cancer using FISH, in some embodiments, a fluorescent probe is developed that binds specifically to the gene of interest, such as the FGFRl gene. In some such embodiments, this gene specific probe is hybridized to a cancer sample and the copy number determined by counting the number of fluorescent signals present per cell using fluorescence microscopy. For a normal diploid cell, the majority of genes will have a copy number of two (exceptions exist when the gene is present on one of the sex chromosomes rather than an autosome or the cell is undergoing division and the genome replicated). If more than two signals are detected in a cell, in certain instances, the gene may be amplified.

[0168] Dual color FISH may also be used for assessing gene amplification in cancer. In some embodiments, a reference probe that binds to the centromere region of the chromosome on which the gene of interest is located can be used as a control. In some instances, the centromere (CEN) region of a chromosome is considered to be genomically stable and is therefore assumed to be representative of the entire chromosome. CEN copy number can therefore, in some embodiments, assist in distinguishing focal gene amplification from increased gene copy number resulting from polysomy (>3 copies of the chromosome centromere) of the chromosome. Gene amplification can be distinguished from polysomy, in some embodiments, by calculating the ratio the signal from the gene-of-interest probe / signal from the centromere probe. For a normal diploid cell, where the gene of interest in located on an autosome, this ratio is typically 1. In some embodiments, a ratio of >1 is indicative of gene amplification. In some embodiments, a probe to a chromosomal reference gene can be used in place of, or in addition to, a centromere probe (see, e.g., Tse et al. (2011) J. Clin. Oncol. 29: 4168-74). In some embodiments, the selected reference gene is also on chromosome 8. In some embodiments, the reference gene is located close to the centromere of chromosome 8. In some embodiments, the reference sequence comprises non-coding DNA on chromosome 8.

[0169] In some embodiments, FISH allows the determination of multiple parameters of gene amplification, including, but not limited to, the fraction of cells with an amplified gene, the amplification levels within various subpopulations of cells, and the amplification pattern within a cell (for example, a clustered signal versus multiple scattered signals). In some embodiments, the ratio of the copy number of the gene of interest to the centromere reference for each cancer cell is determined. In some such embodiments, the mean ratio for a particular sample or subset of cells in a sample is then calculated. A mean ratio of greater than two is generally considered to indicate gene amplification, whereas signals between 1.5 to 2 may indicate low-level amplification. In some embodiments, cells that have a greater copy number of the gene of interest than a reference control probe are considered amplified (see, e.g., Kobayashi et al. (2002) Hum. Pathol. 33 : 21-8; and Kunitomo et al. (2002) Pathol. Int. 52: 451-7). In some embodiments, single-color FISH is used to determine the copy number of a gene of interest without a chromosomal reference probe control. In some such embodiments, four or more copies of the gene per nucleus is considered to be gene amplification (see, e.g., Couturier et al. (2000) Mod. Pathol. 13: 1238-43; Jacobs et al. (1999) J. Clin. Oncol. 17: 1974-82; Wang et al. (2000) J. Clin. Pathol. 53 : 374-81).

[0170] Any suitable method of determining protein overexpression (FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and/or ETV4 overexpression) may be used. In certain embodiments, the expression of proteins in a sample is examined using immunohistochemistry ("IHC") and staining protocols. Immunohistochemical staining of tissue sections has been shown to be a reliable method of assessing or detecting presence of proteins in a sample.

Immunohistochemistry techniques utilize an antibody to probe and visualize cellular antigens in situ, generally by chromogenic or fluorescent methods.

[0171] The tissue sample may be fixed (i.e. preserved) by conventional methodology (See e.g., "Manual of Histological Staining Method of the Armed Forces Institute of Pathology," 3^rd edition (1960) Lee G. Luna, HT (ASCP) Editor, The Blakston Division McGraw-Hill Book Company, New York; The Armed Forces Institute of Pathology Advanced Laboratory Methods in Histology and Pathology (1994) Ulreka V. Mikel, Editor, Armed Forces Institute of Pathology, American Registry of Pathology, Washington, D.C.). One of skill in the art will appreciate that the choice of a fixative is determined by the purpose for which the sample is to be histologically stained or otherwise analyzed. One of skill in the art will also appreciate that the length of fixation depends upon the size of the tissue sample and the fixative used. By way of example, neutral buffered formalin, Bouin's or paraformaldehyde, may be used to fix a sample.

[0172] Generally, the sample is first fixed and is then dehydrated through an ascending series of alcohols, infiltrated and embedded with paraffin or other sectioning media so that the tissue sample may be sectioned. Alternatively, one may section the tissue and fix the sections obtained. By way of example, the tissue sample may be embedded and processed in paraffin by conventional methodology (See e.g., "Manual of Histological Staining Method of the Armed Forces Institute of Pathology", supra). Examples of paraffin that may be used include, but are not limited to, Paraplast, Broloid, and Tissuemay. Once the tissue sample is embedded, the sample may be sectioned by a microtome or the like (See e.g., "Manual of Histological Staining Method of the Armed Forces Institute of Pathology", supra). By way of example for this procedure, sections may range from about three microns to about five microns in thickness. Once sectioned, the sections may be attached to slides by several standard methods. Examples of slide adhesives include, but are not limited to, silane, gelatin, poly-L-lysine and the like. By way of example, the paraffin embedded sections may be attached to positively charged slides and/or slides coated with poly-L-lysine.

[0173] If paraffin has been used as the embedding material, the tissue sections are generally deparaffinized and rehydrated to water. The tissue sections may be deparaffinized by several conventional standard methodologies. For example, xylenes and a gradually descending series of alcohols may be used (See e.g., "Manual of Histological Staining Method of the Armed Forces Institute of Pathology", supra). Alternatively, commercially available deparaffinizing non-organic agents such as Hemo-De7 (CMS, Houston, Tex.) may be used.

[0174] In some embodiments, subsequent to the sample preparation, a tissue section may be analyzed using IHC. IHC may be performed in combination with additional techniques such as morphological staining and/or fluorescence in-situ hybridization. Two general methods of IHC are available; direct and indirect assays. According to the first assay, binding of antibody to the target antigen is determined directly. This direct assay uses a labeled reagent, such as a fluorescent tag or an enzyme-labeled primary antibody, which can be visualized without further antibody interaction. In a typical indirect assay, unconjugated primary antibody binds to the antigen and then a labeled secondary antibody binds to the primary antibody. Where the secondary antibody is conjugated to an enzymatic label, a chromogenic or fluorogenic substrate is added to provide visualization of the antigen. Signal amplification occurs because several secondary antibodies may react with different epitopes on the primary antibody.

[0175] The primary and/or secondary antibody used for immunohistochemistry typically will be labeled with a detectable moiety. Numerous labels are available which can be generally grouped into the following categories: (a) Radioisotopes, such as ³⁵S, ¹⁴C, ¹²⁵1, ³H, and ¹³¹I. The antibody can be labeled with the radioisotope using the techniques described in Current Protocols in Immunology, Volumes 1 and 2, Coligen et al, Ed. Wiley-Interscience, New York, N.Y., Pubs. (1991) for example and radioactivity can be measured using scintillation counting, (b) Colloidal gold particles, (c) Fluorescent labels including, but are not limited to, rare earth chelates (europium chelates), Texas Red, rhodamine, fluorescein, dansyl, Lissamine, umbelliferone, phycocrytherin, phycocyanin, or commercially available fluorophores such SPECTRUM ORANGE7 and SPECTRUM GREEN7 and/or derivatives of any one or more of the above. The fluorescent labels can be conjugated to the antibody using the techniques disclosed in Current Protocols in Immunology, supra, for example, fluorescence can be quantified using a fluorimeter. (d) Various enzyme-substrate labels are available and U.S. Pat. No. 4,275, 149 provides a review of some of these. The enzyme generally catalyzes a chemical alteration of the chromogenic substrate that can be measured using various techniques. For example, the enzyme may catalyze a color change in a substrate, which can be measured spectrophotometrically. Alternatively, the enzyme may alter the fluorescence or chemiluminescence of the substrate. Techniques for quantifying a change in fluorescence are described above. The chemiluminescent substrate becomes electronically excited by a chemical reaction and may then emit light which can be measured (using a chemiluminometer, for example) or donates energy to a fluorescent acceptor.

Examples of enzymatic labels include luciferases (e.g., firefly luciferase and bacterial luciferase; U.S. Pat. No. 4,737,456), luciferin, 2,3-dihydrophthalazinediones, malate dehydrogenase, urease, peroxidase such as horseradish peroxidase (HRPO), alkaline phosphatase, .beta.-galactosidase, glucoamylase, lysozyme, saccharide oxidases (e.g., glucose oxidase, galactose oxidase, and glucose-6-phosphate dehydrogenase), heterocyclic oxidases (such as uricase and xanthine oxidase), lactoperoxidase, microperoxidase, and the like.

Techniques for conjugating enzymes to antibodies are described in O'Sullivan et al, Methods for the Preparation of Enzyme- Antibody Conjugates for use in Enzyme Immunoassay, in Methods in Enzym. (ed. J. Langone & H. Van Vunakis), Academic press, New York, 73: 147- 166 (1981).

[0176] Examples of enzyme-substrate combinations include, for example: (i) Horseradish peroxidase (HRPO) with hydrogen peroxidase as a substrate, wherein the hydrogen peroxidase oxidizes a dye precursor (e.g., orthophenylene diamine (OPD) or 3,3',5,5'- tetramethyl benzidine hydrochloride (TMB)); (ii) alkaline phosphatase (AP) with para- Nitrophenyl phosphate as chromogenic substrate; and (iii) .beta.-D-galactosidase (.beta.-D- Gal) with a chromogenic substrate (e.g., p-nitrophenyl-.beta.-D-galactosidase) or fluorogenic substrate (e.g., 4-methylumbelliferyl-.beta.-D-galactosidase).

[0177] Numerous other enzyme-substrate combinations are available to those skilled in the art. For a general review of these, see U.S. Pat. Nos. 4,275, 149 and 4,318,980.

Sometimes, the label is indirectly conjugated with the antibody. The skilled artisan will be aware of various techniques for achieving this. For example, the antibody can be conjugated with biotin and any of the four broad categories of labels mentioned above can be conjugated with avidin, or vice versa. Biotin binds selectively to avidin and thus, the label can be conjugated with the antibody in this indirect manner. Alternatively, to achieve indirect conjugation of the label with the antibody, the antibody is conjugated with a small hapten and one of the different types of labels mentioned above is conjugated with an anti-hapten antibody. Thus, indirect conjugation of the label with the antibody can be achieved.

[0178] Aside from the sample preparation procedures discussed above, further treatment of the tissue section prior to, during or following IHC may be desired. For example, epitope retrieval methods, such as heating the tissue sample in citrate buffer may be carried out (see, e.g., Leong et al. Appl. Immunohistochem. 4(3):201 (1996)).

[0179] Following an optional blocking step, the tissue section is exposed to primary antibody for a sufficient period of time and under suitable conditions such that the primary antibody binds to the target protein antigen in the tissue sample. Appropriate conditions for achieving this can be determined by routine experimentation. The extent of binding of antibody to the sample is determined by using any one of the detectable labels discussed above. In some embodiments, the label is an enzymatic label (e.g. HRPO) which catalyzes a chemical alteration of the chromogenic substrate such as 3,3'-diaminobenzidine chromogen. In one embodiment, the enzymatic label is conjugated to antibody which binds specifically to the primary antibody (e.g. the primary antibody is rabbit polyclonal antibody and secondary antibody is goat anti-rabbit antibody).

[0180] Specimens thus prepared may be mounted and coverslipped. Slide evaluation is then determined, e.g., using a microscope, and staining intensity criteria, routinely used in the art, may be employed.

[0181] In some embodiments, when IHC is used, a tiered system of staining is used to determine whether a cell or collection of cells overexpresses FGFR1 protein. For example, in some embodiments, a four-tiered system is used in which the tiers are no staining, 1+, 2+, and 3+, where 1+, 2+, and 3+ indicate increasing levels of staining, respectively. In some such embodiments, greater than 1+, greater than 2+, or greater than 3+ may be used to indicate FGFR1 protein overexpression. As a nonlimiting example, if a particular cell type typically shows no staining for FGFR1 in an IHC assay, then any staining in that IHC assay (i.e., 1+, 2+, or 3+) may be indicative as protein overexpression. As a further nonlimiting example, if a particular cell type typically shows little to no staining for FGFR1 in an IHC assay, then any staining above 1+ in that IHC assay (i.e., 2+ or 3+) may be indicative as protein overexpression. One skilled in the art can determine the staining level that indicates protein overexpression depending on the particular IHC assay (including the particular antibody), the cell type, etc.

[0182] Any suitable method of determining mRNA overexpression (such as FGFR1 overexpression, and/or FGF2 overexpression, and/or DKK3 overexpression, and/or FGF18 overexpression, and/or ETV4 overexpression) may be used. Methods for the evaluation of mRNAs in cells are well known and include, for example, hybridization assays using complementary DNA probes (such as in situ hybridization using labeled riboprobes specific for FGFR1, FGF2, DKK3, FGF18, or ETV4 Northern blot and related techniques) and various nucleic acid amplification assays (such as RT-PCR using complementary primers specific for FGFR1, FGFRlIIIc, FGFR3IIIc, FGF2, DKK3, FGF18, or ETV4 and other amplification type detection methods, such as, for example, branched DNA, SISBA, TMA and the like).

[0183] Tissue or cell samples from mammals can be conveniently assayed for mRNAs using Northern, dot blot or PCR analysis. For example, RT-PCR assays such as quantitative PCR assays are well known in the art. In some embodiments, mRNA expression levels are levels quantified using real-time qRT-PCR. In some embodiments of the invention, a method for detecting a target mRNA in a biological sample comprises producing cDNA from the sample by reverse transcription using at least one primer; amplifying the cDNA so produced using a target polynucleotide as sense and antisense primers to amplify target cDNAs therein; and detecting the presence of the amplified target cDNA. In addition, such methods can include one or more steps that allow one to determine the levels of target mRNA in a biological sample (e.g., by simultaneously examining the levels a comparative control mRNA sequence of a "housekeeping" gene such as an actin family member). Optionally, the sequence of the amplified target cDNA can be determined.

[0184] Optional methods of the invention include protocols which examine or detect mRNAs, such as target mRNAs, in a tissue or cell sample by microarray technologies. Using nucleic acid microarrays, test and control mRNA samples from test and control tissue samples are reverse transcribed and labeled to generate cDNA probes. The probes are then hybridized to an array of nucleic acids immobilized on a solid support. The array is configured such that the sequence and position of each member of the array is known.

Hybridization of a labeled probe with a particular array member indicates that the sample from which the probe was derived expresses that gene. Differential gene expression analysis of disease tissue can provide valuable information. Microarray technology utilizes nucleic acid hybridization techniques and computing technology to evaluate the mRNA expression profile of thousands of genes within a single experiment, (see, e.g., WO 01/75166 published Oct. 1 1, 2001; (see, for example, U.S. Pat. No. 5,700,637, U.S. Pat. No. 5,445,934, and U.S. Pat. No. 5,807,522, Lockart, Nature Biotechnology, 14: 1675-1680 (1996); Cheung, V. G. et al, Nature Genetics 21(Suppl): 15-19 (1999) for a discussion of array fabrication). DNA microarrays are miniature arrays containing gene fragments that are either synthesized directly onto or spotted onto glass or other substrates. Thousands of genes are usually represented in a single array. A typical microarray experiment involves the following steps: 1) preparation of fluorescently labeled target from RNA isolated from the sample, 2) hybridization of the labeled target to the microarray, 3) washing, staining, and scanning of the array, 4) analysis of the scanned image and 5) generation of gene expression profiles.

Currently two main types of DNA microarrays are being used: oligonucleotide (usually 25 to 70 mers) arrays and gene expression arrays containing PCR products prepared from cDNAs. In forming an array, oligonucleotides can be either prefabricated and spotted to the surface or directly synthesized on to the surface (in situ). In some embodiments, a DNA microarray is a single-nucleotide polymorphism (SNP) microarrays, e.g., Affymetrix^® SNP Array 6.0.

[0185] The Affymetrix GeneChip^® system is a commercially available microarray system which comprises arrays fabricated by direct synthesis of oligonucleotides on a glass surface. Probe/Gene Arrays: Oligonucleotides, usually 25 mers, are directly synthesized onto a glass wafer by a combination of semiconductor-based photolithography and solid phase chemical synthesis technologies. Each array contains up to 400,000 different oligos and each oligo is present in millions of copies. Since oligonucleotide probes are synthesized in known locations on the array, the hybridization patterns and signal intensities can be interpreted in terms of gene identity and relative expression levels by the Affymetrix Microarray Suite software. Each gene is represented on the array by a series of different oligonucleotide probes. Each probe pair consists of a perfect match oligonucleotide and a mismatch oligonucleotide. The perfect match probe has a sequence exactly complimentary to the particular gene and thus measures the expression of the gene. The mismatch probe differs from the perfect match probe by a single base substitution at the center base position, disturbing the binding of the target gene transcript. This helps to determine the background and nonspecific hybridization that contributes to the signal measured for the perfect match oligo. The Microarray Suite software subtracts the hybridization intensities of the mismatch probes from those of the perfect match probes to determine the absolute or specific intensity value for each probe set. Probes are chosen based on current information from Genbank and other nucleotide repositories. The sequences are believed to recognize unique regions of the 3' end of the gene. A GeneChip Hybridization Oven ("rotisserie" oven) is used to carry out the hybridization of up to 64 arrays at one time. The fluidics station performs washing and staining of the probe arrays. It is completely automated and contains four modules, with each module holding one probe array. Each module is controlled independently through Microarray Suite software using preprogrammed fluidics protocols. The scanner is a confocal laser fluorescence scanner which measures fluorescence intensity emitted by the labeled cRNA bound to the probe arrays. The computer workstation with Microarray Suite software controls the fluidics station and the scanner. Microarray Suite software can control up to eight fluidics stations using preprogrammed hybridization, wash, and stain protocols for the probe array. The software also acquires and converts hybridization intensity data into a presence/absence call for each gene using appropriate algorithms. Finally, the software detects changes in gene expression between experiments by comparison analysis and formats the output into .txt files, which can be used with other software programs for further data analysis.

EXAMPLES

[0186] The examples discussed below are intended to be purely exemplary of the invention and should not be considered to limit the invention in any way. The examples are not intended to represent that the experiments below are all or the only experiments performed. It is understood that various other embodiments may be practiced, given the general description provided above. Efforts have been made to ensure accuracy with respect to numbers used (for example, amounts, temperature, etc.) but some experimental errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, molecular weight is weight average molecular weight, temperature is in degrees Centigrade, and pressure is at or near atmospheric.

Example 1: FGFR1-ECD.339-Fc inhibits proliferation of FGFR1 amplified lung cancer cell lines in tissue culture

[0187] A panel of lung cancer cell lines displaying potential amplification of the FGFR1 gene was identified using CONAN (http://www.sanger.ac.uk/cgi- bin/genetics/CGP/conan/search.cgi) and Tumorscape

(http://www.broadinstitute.org/tumorscape/pages/portalHome.jsf). CONAN and Tumorscape represent public data mining tools to extract gene copy number information for predefined or user defined loci across the SNP6.0 dataset of cancer. Lung cancer cell lines DMS53, DMSl 14, NCI-H1581 and NCI-H520 were identified as having potential amplification of the FGFR1 gene (>4 copies/cell) and were selected for further analysis. Human small cell lung cancer (SCLC) cell lines DMS53 and DMSl 14 were purchased from ATCC (Manassas, VA; Cat. No. CRL-2062; Cat. No. CRL-2066, respectfully). The cells were cultured in

Waymouth's MB 752/1 medium + 10% FBS + 2mM L-glutamine at 37°C in a humidified atmosphere with 5% CO₂. Human non-small cell lung cancer (NSCLC) cell line NCI-H1581 was purchased from ATCC (Manassas, VA; Cat. No. CRL-5878) and cultured in ACL-4 medium (serum-free). The base medium for NCI-H1581 is DMEM: F12 (50/50 mix) with the following components to the base medium: 0.02 mg/ml insulin, 0.01 mg/ml transferrin, 25 nM sodium selenite (final cone), 50 nM Hydrocortisone (final cone), 1 ng/ml Epidermal Growth Factor (final cone), 0.01 mM ethanolamine (final cone), 0.01 mM

phosphorylethanolamine (final cone), 100 pM triiodothyronine (final cone), 0.5% (w/v) bovine serum albumin (final cone), 0.5 mM sodium pyruvate (final cone) and 4.5mM L- glutamine. Cells were grown at 37°C in a humidified atmosphere with 5% C02. Human non- small cell lung cancer (NSCLC) cell line NCI-H520 was purchased from ATCC (Manassas, VA; Cat. No. HTB-182). The cells were cultured in RPMI-1640 Medium + 10% FBS + 2mM L-glutamine at 37°C in a humidified atmosphere with 5% CO₂.

[0188] Amplification status of the FGFRl gene in the cell lines was confirmed by QuantiGene® Plex DNA Assay (Panomics). The QuantiGene Plex DNA Assay is a hybridization-based assay using xMAP® Luminex® magnetic beads. Individual, bead-based, oligonucleotide probe sets (including capture, capture extenders, blockers, and label probes) specific for FGFRl (NM_023110), ALB (NM_000477) and DCK (NM_000788) genes were designed to prevent cross-reactivity (Panomics, Affymetrix, Santa Clara, CA). ALB and DCK were used as reference genes for normalizing FGFRl copy number. Cell samples were lysed to release DNA and incubated overnight with FGFRl target specific probe sets. On the second day a signal amplification tree was built via sequential hybridization of PreAmplifier (PreAmp), Amplifier (Amp) and biotinylated Label Probe (LP). The signal was detected by adding phycoerythrin streptavidin (SAPE) substrate. SAPE fluorescence was detected at 575nm for each capture bead using a Luminex 200 flow cytometer instrument (Luminex, Austin, TX). All data were normalized to the reference genes and expressed as a ratio (FGFRL ALB). Data for the four cell lines is shown in Table 2.

Table 2. FGFRl gene amplification in lung cancer cell lines

TGI = tumor growth inhibition. [0189] To determine the impact of FGFR1-ECD.339-Fc on lung cancer cell lines in tissue culture, cells were plated in a Microtest™ 96-well tissue culture plate (Becton Dickenson, Franklin Lakes, NJ) at a density of 5xl0³ cells/well in medium containing 10%, 1% or 0.1% FBS in the presence or absence of 15μ πύ FGFR1-ECD.339-Fc (SEQ ID NO: 6) or an unrelated ECD-Fc fusion protein (as a negative control). Plates were incubated at 37°C at 5% CO₂ for 4 days and then assayed to determine the impact of FGFR1-ECD.339-Fc on cell number and proliferation.

[0190] To determine cell number the CellTiter-Glo^® Luminescent Cell Viability Assay (Promega, Madison, WI) was employed. CellTiter-Glo^® is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. In brief, CellTiter-Glo^® Reagent was added to each well of the tissue culture plate at a volume equal to the volume of cell culture medium present in each well (ΙΟΟμΙ), the contents mixed for 2 minutes on an orbital shaker to induce cell lysis and then the plate incubated for 10 minutes at room temperature. Luminescence was then determined on an EnVision™ Multilabel Plate Reader (PerkinElmer, Boston, MA) with a 0.2 second integration time. Results were expressed as relative light units (RLU) / well.

[0191] Results from the CellTiter-Glo^® assay demonstrated that cell number was significantly (P = >0.01) reduced by FGFR1-ECD.339-Fc incubation in all four cell lines with FGFRl amplification (FIG. 1A-D show NCI-H 1581, NCI-H520, DMS53, and DMS114, respectively). -values were determined using an unpaired t-test. See Mathematical Statistics and Data Analysis. 1988, Wadsworth & Brooks, Pacific Grove, CA.

[0192] To determine the impact of FGFR1-ECD.339-Fc on cell proliferation the tritiated thymidine ([3H]-TdR) incorporation assay was employed. Following incubation of lung cancer cell lines with FGFR1-ECD.339-Fc or an unrelated ECD-Fc negative control, tritiated thymidine ([3H]-TdR; PerkinElmer, Boston, MA) was added at activity of 1 μθϊ/ννεΐΐ. After 16-h exposure, tritiated thymidine incorporation was assessed. Cells were washed with Dulbecco's phosphate-buffered saline (DPBS; Mediatech, Inc.) and removed from cell culture surface by incubation with trypsin-EDTA (Mediatech, Inc.). The cell suspension (200 μΐ) was then removed from the tissue culture plate using a FilterMate harvester (PerkinElmer) and filtered through a UniFilter-96 GF/B (PerkinElmer) plate. Cells were lysed using 95% ethanol and 40 μΐ of Microscint 40 (PerkinElmer) scintillant fluid added per well. Thymidine incorporation was determined as counts per minute (cpm) on a Topcount NXT (PerkinElmer) scintillation counter. Results were expressed as cpm/well. [0193] In the tritiated thymidine incorporation assay, FGFR1-ECD.339-Fc reduced NCI- H1581, NCI-H520, DMS53, and DMS1 14 cell proliferation by 85, 33, 52 and 81 %, respectively (FIG. 2A-D, respectively). The control ECD-Fc demonstrated no impact on cell proliferation in any cell line. An additional lung tumor cell line, NCI-H1703 ( SCLC;

FGFRl gene copy number: 6 copies/cell) was also tested in the tritiated thymidine incorporation assay following incubation with FGFR1-ECD.339-Fc, as described above. FGFR1-ECD.339-Fc reduced NCI-H 1703 proliferation by 15%.

[0194] Results from the tritiated thymidine incorporation assay demonstrate that cell proliferation was significantly (* indicates P = >0.05) reduced by FGFR1-ECD.339-Fc incubation in all four cell lines with FGFRl gene amplification (FIG. 2). P- values were determined using an unpaired t-test. See Mathematical Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA. The control ECD Fc had little no impact on cell proliferation in any cell line.

[0195] Percent reduction in CellTiterGlo relative light units (RLU) in the presence of FGFR1-ECD.339-Fc was averaged across all FBS concentrations examined for each of the four FGFRl gene-amplified lung cancer cell lines and was compared to a panel of lung cancer cell lines without FGFRl gene amplification (FIG. 3). Lung cancer cell lines without FGFRl gene amplification examined in this experiment included NCI-H838, NCI-H1793, A549, Calu-1, NCI-H226, NCI-H441, NCI-H460, NCI-H522 and NCI-H2126. Non- amplified cell lines were purchased from ATTC (Manassas, VA) and cultured according to supplier instructions. Lung cancer cell lines with FGFRl gene amplification on average had a 46.25 % reduction in cell number, as assessed by CellTiterGlo, with the addition of FGFR1- ECD.339-Fc compared to addition of control ECD-Fc. In comparison, lung cancer cell lines without FGFRl gene amplification displayed on average a 9.33 % decrease in cell number, as assessed by CellTiterGlo, on addition of FGFR1-ECD.339-Fc compared to addition of control ECD-Fc. This difference between the impact on cell number of FGFR1-ECD.339-Fc on FGFRl gene amplified and non-amplified lung cancer cell lines was statistically significant ( =0.0039).

[0196] The impact of FGFR1-ECD.339-Fc on cell proliferation as assessed by tritiated thymidine incorporation was also compared between FGFRl gene amplified and non- amplified lung cancer cell lines (FIG. 4). An average percent reduction in cell proliferation with FGFR1-ECD.339-Fc addition was determined across all FBS concentrations examined for each FGFRl gene amplified cell line and the panel of non-FGFRl gene amplified cell lines indicated above. Lung cancer cell lines with FGFRl gene amplification on average had a 62.75% reduction in CPM with the addition of FGFR1-ECD.339-Fc compared to addition of control ECD-Fc. In comparison, lung cancer cell lines without FGFRl gene amplification displayed on average a 17.0 % decrease in CPM, on addition of FGFR1-ECD.339-Fc compared to addition of control ECD-Fc. This difference between FGFRl gene amplified and non-amplified lung cancer cell lines was statistically significant ( =0.0088).

Example 2: Administration of FGFR1-ECD.339-Fc inhibits tumor growth in the DMS53 small cell lung cancer (SCLC) xenograft model

[0197] Six week old female SCID mice were purchased from Charles River Laboratories

(Wilmington, MA) and were acclimated for 1 week before the start of the study. Human small cell lung cancer (SCLC) cell line DMS53 was used as the tumor model and was purchased from ATCC (Manassas, VA; Cat. No. CRL-2062). The cells were cultured for three passages in Waymouth's MB 752/1 medium + 10% FBS + 2mM L-glutamine at 37°C in a humidified atmosphere with 5% CO₂. When the cultured cells reached 85-90% confluence, cells were harvested and resuspended in cold Ca²⁺ and Mg²⁺ free phosphate buffered saline

(PBS) containing 50% Matrigel at 5 xlO⁷ cells per milliliter. The cells were implanted subcutaneous ly over the right flank of the mice at 5xl0⁶ cells/100 μΐ/mouse. One day following cell implantation mice were sorted and randomized (n=10) and treatment initiated according to Table 3, below.

[0198] FGFR 1 -ECD .339 -Fc was formulated in PBS at 3 mg/ml and administered intraperitoneally (i.p.) at 15 mg/kg (300 μg/100 μΐ/mouse) twice a week for four weeks. Human albumin was purchased from Grifols USA (Los Angeles, CA; Cat. No. NDC 61953- 0002-1), diluted to a working stock (3 mg/ml) with 0.9% sodium chloride, and was used as negative control at 300 μg/100 μΐ/mouse (15 mg/kg) administered twice a week for four weeks.

Table 3. Dosing groups

[0199] Tumor sizes were measured in each mouse on days 7, 14, 21, 28, 35 and 39 following the day of tumor cell inoculation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula: Tumor size (mm³) = (width (mm) x length (mm))²/2

Mice were euthanized as a "cancer death" when the subcutaneous tumor volumes exceeded 2000 mm³ or when the tumors became excessively necrotic.

[0200] FIG. 5 shows the results of this experiment. Mice that received FGFR1-ECD.339-

Fc showed a 64% reduction of tumor growth compared to albumin-treated animals.

Comparison of DMS 53 tumor volume at day 37 in the FGFR1-ECD.339-Fc treatment group and vehicle treated group indicated that this result was statistically significant (P = 0.003). P- values were calculated using an ANOVA analysis. See, e.g., Mathematical Statistics and

Data Analysis. 1988, Wadsworth & Brooks, Pacific Grove, CA. This analysis demonstrated that FGFR1-ECD.339-Fc significantly reduced tumor growth in the lung cancer cell line

DMS53, which has amplification of the gene encoding the FGFR1 receptor.

Example 3: Administration of FGFR1-ECD.339-Fc inhibits tumor growth in the DMS114 small cell lung cancer (SCLC) xenograft model

[0201] Six week old female SCID mice were purchased from Charles River Laboratories

(Wilmington, MA) and were acclimated for 1 week before the start of the study. Human small cell lung cancer (SCLC) cell line DMS 114 was used as the tumor model and was purchased from ATCC (Manassas, VA; Cat. No. CRL-2066). The cells were cultured for three passages in Waymouth's MB 752/1 medium + 10% FBS + 2mM L-glutamine at 37°C in a humidified atmosphere with 5% CO₂. When the cultured cells reached 85-90% confluence, cells were harvested and resuspended in cold Ca²⁺ and Mg²⁺ free phosphate buffered saline

(PBS) containing 50% Matrigel at 5 xlO⁷ cells per milliliter. The cells were implanted subcutaneous ly over the right flank of the mice at 5xl0⁶ cells/100 μΐ/mouse. One day following cell implantation mice were sorted and randomized (n=10) and treatment initiated as described in Example 2, above.

[0202] Tumor sizes were measured in each mouse on days 3, 10, 16, 19, 24, 27, and 31 following the day of tumor cell inoculation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula:

Tumor size (mm³) = (width (mm) x length (mm))²/2

[0203] FIG. 6 shows the results of this experiment. Mice that received FGFR1-ECD.339- Fc showed a 64% reduction of tumor growth compared to albumin-treated animals.

Comparison of DMS 114 tumor volume at day 31 in the FGFR1-ECD.339-Fc treatment group and vehicle treated group indicated that this result was statistically significant (P = 0.002). -values were calculated using an ANOVA analysis. See, e.g.. Mathematical

Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA. This analysis demonstrated that FGFR1-ECD.339-Fc significantly reduced tumor growth in the lung cancer cell line DMS1 14, which has amplification of the gene encoding the FGFR1 receptor.

Example 4: Administration of FGFR1-ECD.339-Fc inhibits tumor growth in the NCI-H1581 non-small cell lung cancer (NSCLC) xenograft model

[0204] Six week old female SCID mice were purchased from Charles River Laboratories (Wilmington, MA) and were acclimated for 1 week before the start of the study. Human non- small cell lung cancer (NSCLC) cell line NCI-H1581 was used as the tumor model and was purchased from ATCC (Manassas, VA; Cat. No. CRL-5878). The cells were cultured for three passages in ACL-4 medium (serum-free). The base medium for this cell line is DMEM: F12 (50/50 mix) with the following components to the base medium: 0.02 mg/ml insulin, 0.01 mg/ml transferrin, 25 nM sodium selenite (final cone), 50 nM Hydrocortisone (final cone), 1 ng/ml Epidermal Growth Factor (final cone), 0.01 mM ethanolamine (final cone), 0.01 mM phosphorylethanolamine (final cone), 100 pM triiodothyronine (final cone), 0.5% (w/v) bovine serum albumin (final cone), 0.5 mM sodium pyruvate (final cone) and 4.5mM L-glutamine. Cells were grown at 37°C in a humidified atmosphere with 5% CO₂. When the cultured cells reached 85-90% confluence, cells were harvested and resuspended in cold Ca²⁺ and Mg²⁺ free phosphate buffered saline (PBS) containing 50% Matrigel at 5 xlO⁷ cells per milliliter. The cells were implanted subcutaneous ly over the right flank of the mice at 5xl0⁶ cells/100 μΐ/mouse. One day following cell implantation mice were sorted and randomized (n=10) and treatment initiated as described in Example 2, above.

[0205] Tumor sizes were measured in each mouse on days 7, 10, 14, 17, 21, 25 and 31 following the day of tumor cell inoculation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula:

Tumor size (mm³) = (width (mm) x length (mm))²/2

[0206] FIG. 7 shows the results of this experiment. Mice that received FGFR1-ECD.339- Fc showed a 74% reduction of tumor growth compared to albumin-treated animals.

Comparison of NCI-H1581 tumor volume at day 31 in the FGFR1-ECD.339-Fc treatment group and vehicle treated group indicated that this result was statistically significant (P < 0.001). -values were calculated using an ANOVA analysis. See, e.g.. Mathematical Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA. This analysis demonstrated that FGFR1-ECD.339-Fc significantly reduced tumor growth in the lung cancer cell line NCI-H1581, which has amplification of the gene encoding the FGFR1 receptor.

Example 5: Administration of FGFR1-ECD.339-Fc inhibits tumor growth in the NCI-H520 non-small cell lung cancer (NSCLC) xenograft model

[0207] Six week old female SCID mice were purchased from Charles River Laboratories (Wilmington, MA) and were acclimated for 1 week before the start of the study. Human non- small cell lung cancer (NSCLC) cell line NCI-H520 was used as the tumor model and was purchased from ATCC (Manassas, VA; Cat. No. HTB-182). The cells were cultured for three passages in RPMI-1640 Medium+ 10% FBS + 2mM L-glutamine at 37°C in a humidified atmosphere with 5% CO₂. When the cultured cells reached 85-90% confluence, cells were harvested and resuspended in cold Ca²⁺ and Mg²⁺ free phosphate buffered saline (PBS) containing 50% Matrigel at 5 xlO⁷ cells per milliliter. The cells were implanted subcutaneous ly over the right flank of the mice at 5xl0⁶ cells/100 μΐ/mouse. One day following cell implantation mice were sorted and randomized (n=10) and treatment initiated as described below.

[0208] FGFR1-ECD.339-FC was formulated in PBS at 3 mg/ml and administered intraperitoneally (i.p.) at 20 mg/kg (400 μg/125 μΐ/mouse) twice a week for four weeks. Human albumin was purchased from Grifols USA (Los Angeles, CA; Cat. No. NDC 61953- 0002-1), diluted to a working stock (3 mg/ml) with 0.9% sodium chloride, and was used as negative control at 400 μg/125 μΐ/mouse (20 mg/kg) administered twice a week for six weeks.

[0209] Tumor sizes were measured in each mouse on days 11, 18, 25, 32, 39 and 46 following the day of tumor cell inoculation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula:

Tumor size (mm³) = (width (mm) x length (mm))²/2

[0210] FIG. 8 shows the results of this experiment. Mice that received FGFR1-ECD.339- Fc showed a 47% reduction of tumor growth compared to albumin-treated animals.

Comparison of NCI-H520 tumor volume at day 46 in the FGFR1-ECD.339-Fc treatment group and vehicle treated group indicated that this result was statistically significant (P < 0.01). P- values were calculated using an ANOVA analysis. See, e.g., Mathematical Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA. This analysis demonstrated that FGFR1-ECD.339-Fc significantly reduced tumor growth in the lung cancer cell line NCI-H520, which has amplification of the gene encoding the FGFRl receptor.

[0211] Efficacy of FGFR1-ECD.339-Fc treatment in one additional xenograft model, using non-small cell lung cancer (NSCLC) cell line NCI-H1703, was tested in a similar manner as the SCLC and NSCLC cell lines described above. Mice that received FGFRl - ECD.339-Fc showed a 31% reduction of tumor growth compared to albumin-treated animals. It is noted that NCI-H1703 cell line contains a drug-sensitive PDGFRA / PDGFC genomic amplification in addition to FGFRl amplification, which may be responsible for the modest efficacy observed.

Example 6: Certain lung cancer xenograft models with FGFRl gene

amplification were more sensitive to FGFRl-ECD.339-Fc-mediated growth inhibition than certain n n-FGFRl gene amplified lung cancer xenograft models

[0212] The impact of FGFR1-ECD.339-Fc on tumor growth was compared between FGFRl gene amplified and non-amplified lung cancer xenograft models. Lung cell lines without FGFRl -amplification examined in this experiment were as follows: A549, NCI- H460, NCI-H226, NCI-H2126, NCI-H441, NCI-H358, NCI-H522 and Colo699. Non- amplified cell lines were purchased from ATTC (Manassas, VA) and cultured according to supplier instructions. Lung cancer xenograft models using non-FGFRl gene amplified cell lines were carried out substantially as described in Example 2.

[0213] A panel of patient-derived xenograft (PDX) models of lung cancer without G Ri-amplification was also examined for sensitivity to FGFR1-ECD.339-Fc. PDX xenografts have been transplanted directly from cancer patients into nude mice without in vitro tissue culture. The tumor xenografts retain most of the characteristics of the parental patient tumors including histology and sensitivity to anticancer drugs. Lung PDX models examined were as follows: PDX D35087, PDX D37638, PDX D35376, LXFL-430, LXFE- 937, LXFE-397, LXFA-737 and LXFA-629. Preliminary pathology and patient

characteristics for the lung PDXs examined are outlined in Table 4.

Table 4: Characteristics of lung cancer patient-derived xenograph (PDX) models

LXFA_629 Adeno Lung poorly 59 male T3N2Mx differentiated

LXFA_737 Adeno Lung moderately 56 male T3N2Mx differentiated

PDX Squamous Lung moderately - - T3N0M0 D35087 differentiated

PDX Squamous Lung poorly - - T3N2M0 D37638 differentiated

PDX Squamous Lung moderately - - T2N0M0 D35376 differentiated

[0214] Six week old female SCID mice were purchased from Charles River Laboratories (Wilmington, MA) and were acclimated for 1 week before the start of the study. PDX tumor fragments were obtained from xenografts in serial passage in donor SCID mice. After removal of tumors from donor mice, they were cut into fragments (1-2 mm diameter, ~25 mgs) and placed in RPMI 1640 culture medium until subcutaneous implantation. Recipient mice were anaesthetized by inhalation of isoflurane. A small pocket was formed with blunt forceps and one chunk of tumor PDX was placed in the pocket. The wound was sealed using dermabond glue and a drop of bupivicaine placed on the incision. One day following PDX implantation mice were sorted and randomized (n=10) and treatment initiated as described below.

[0215] FGFR1-ECD.339-Fc was formulated in PBS at 3 mg/ml and administered intraperitoneally (i.p.) at 15 mg/kg (300 μg/100 μΐ/mouse) twice a week for four to eight weeks depending on the growth rate of the PDX tumor implanted. Human albumin was purchased from Grifols USA (Los Angeles, CA; Cat. No. NDC 61953-0002-1), diluted to a working stock (3 mg/ml) with 0.9% sodium chloride, and was used as negative control at 300 μg/100 μΐ/mouse (15 mg/kg) administered twice a week for four to eight weeks depending on the growth rate of the PDX tumor implanted.

[0216] Tumor sizes were measured in each mouse on days 11, 18, 25, 32, 39 and 46 following the day of tumor cell inoculation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula:

Tumor size (mm³) = (width (mm) x length (mm))²/2

[0217] Percentage tumor growth inhibition by FGFR1-ECD.339-Fc was determined by area-under-the-curve (AUC) analysis of xenograft growth curves treated with FGFR1- ECD.339-Fc compared to albumin control. FIG. 9 shows a scatterplot of the results of this analysis. Lung cancer xenografts with FGFR1 gene amplification had an average a 56% reduction in tumor growth with FGFR1-ECD.339-Fc treatment. In comparison, lung cancer xenografts without FGFR1 gene amplification displayed an average 22% decrease in xenograft growth with FGFR1-ECD.339-Fc treatment compared to control. The difference in FGFRl-ECD.339-Fc-mediated xenograft inhibition between FGFR1 gene amplified and non- amplified lung cancer xenograft models was statistically significant ( =0.0333).

[0218] Thus, FGFR1 gene amplified tumor cells were found to be more sensitive to FGFR1-ECD.339-Fc administration than tumor cells with a non-amplified FGFR1 gene.

Example 7: FGFR1 overexpression in FGFR1 gene-amplified and non-amplified lung cancer cell lines and xenografts

[0219] The expression of the FGFR1 at the RNA level was compared between FGFR1 gene amplified and non-amplified lung cancer cell lines, xenograft models, and PDX models.

Lung cancer cell lines without FGFR1 gene amplification examined in this experiment were as follows: A549, NCI-H460, NCI-H226, NCI-H2126, NCI-H441, NCI-H358, NCI-H522,

MSTO-21 1H, and Colo699. Non-amplified cell lines were purchased from ATTC

(Manassas, VA) and cultured according to supplier instructions. A panel of patient-derived xenograft (PDX) models of lung cancer without FGFR1 gene amplification was also examined for FGFR1 mRNA expression. Lung PDX models examined were as follows:

PDX D35087, PDX D37638, PDX D35376, LXFL-430, LXFE-937, LXFE-397, LXFA-737, and LXFA-629. Preliminary pathology and patient characteristics for the lung PDXs examined are outlined above in Table 4.

[0220] RNA was extracted from cell lines grown in vitro or tumor xenografts grown in vivo using the RNAeasy® mini kit (cat. No. 74104, Qiagen, Germany). Extracted RNA was treated with DNAse I prior to creating cDNA with random hexamer priming and reverse transcriptase using the QuantiTect Reverse Transcription Kit (cat. No. 20531 1, Qiagen, Germany). Human FGFR1 RNA expression was determined using an FGFR1 QuantiTect Primer Assay (Hs FGFRl l SG, cat. No. QT00102837, Qiagen, Germany) and a human GUSB control reference QuantiTect Primer Assay (Hs GUSB l SG, cat. No. QT00046046, Qiagen, Germany). QuantiTect SYBR Green PCR Kits (cat. No. 204145, Qiagen, Germany) were used to quantify mRNA expression levels using real-time qRT-PCR and an ABI Prism ViiA™ 7 Real-Time PCR System (Applied Biosystems, Foster City, CA). Relative gene expression quantification was calculated according to the comparative Ct method using human GUSB as a reference and commercial RNA controls (Stratagene, La Jolla, CA). Relative quantification was determined according to the formula: 2 (^{ACt sam}P^{le ACt calibrator}). [0221] GUSB-normalized FGFRl RNA expression was compared between lung cancer cell lines (FIG. 10) and xenograft models (FIG. 12) with and without FGFRl gene amplification.

[0222] FIG. 10 shows a scatterplot of FGFRl RNA expression in cell lines with and without FGFRl gene amplification. Lung cancer cell lines with FGFRl gene amplification have a statistically significant increase (P = 0.0114) in FGFRl mRNA expression compared to cell lines without FGFRl gene amplification. FIG. 10 also demonstrates that a sub- population of lung cancer cell lines have high FGFRl mRNA expression in the absence of FGFRl gene amplification. NCI-H226, which has a GUSB normalized gene expression of FGFRl of 1.48, and NCI-H522, which has a GUSB normalized gene expression of FGFRl of 1.26, represent the two uppermost outlier points in the non-amplified lung cancer cell line population.

[0223] NCI-H226 and NCI-H522 were also sensitive to FGFR1-ECD.339-Fc in vitro, having decreased cell proliferation and number using the tritiated thymidine ([3H]-TdR) incorporation assay and CellTiter-Glo® Luminescent Cell Viability Assay (Promega, Madison, WI), respectively. FIG.1 1 A shows results from the CellTiter-Glo® assay for the NCI-H226 cell line, demonstrating that cell number was significantly (* indicates P = >0.05) reduced by FGFR1-ECD.339-Fc incubation in the NCI-H226 cell line, which does not have G Ri-amplification. -values were determined using an unpaired t-test. See, e.g., Mathematical Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA.

[0224] FIG. 1 IB shows results from the tritiated thymidine incorporation assay for the NCI-H226 cell line, demonstrating that cell proliferation was significantly (* indicates P = >0.05) reduced by FGFR1-ECD.339-Fc incubation in the NCI-H226 cell line, which does not have FGFRl gene amplification. -values were determined using an unpaired t-test. The control ECD Fc had little no impact on NCI-H226 cell proliferation.

[0225] Thus, certain lung cancer cell lines that do not have FGFRl gene amplification, but which have FGFRl overexpression, are sensitive to FGFR1-ECD.339-Fc treatment.

[0226] FIG. 12 shows a scatterplot of FGFRl mRNA expression comparing FGFRl gene amplified to non-amplified lung cancer xenografts. Xenograft models with FGFRl gene amplification had a statistically significant (P = 0.0146) increase in FGFRl RNA levels compared to non-amplified cell lines. In addition, in agreement with the in vitro data, a sub- population of lung cancer xenograft models has high FGFRl RNA expression in the absence of FGFRl gene amplification. Xenograft models NCI-H226, NCI-H522 and PDX D35087 represent the 3 outlier points for FGFR1 RNA expression in the non-amplified lung models (FIG.12), with Gt/SS-normalized gene expression levels of 3.70, 3.75 and 4.30, respectively.

[0227] NCI-H226, NCI-H522, and PDX D35087 were also sensitive to FGFR1-ECD.339- Fc in vivo, demonstrating a statistically significant (P < 0.05) reduction in tumor growth of 55, 42 and 57 % respectively with FGFR1-ECD.339-Fc treatment. For PDX D35087, the experiment was carried out substantially as described in Example 6.

[0228] Tumor sizes were measured in each mouse on days 26, 35, 41 and 45 following the day of PDX D35087 implantation. The length and width of each tumor was measured using calipers and the tumor size calculated according to the formula:

[0229] Tumor size (mm³) = (width (mm) x length (mm))²/2

[0230] FIG. 13 shows the results of this experiment. Mice that received FGFR1-ECD.339- Fc showed an inhibition of tumor growth compared to albumin-treated animals. Comparison of PDX 35087 tumor volume at day 45 in the FGFR1-ECD.339-Fc treatment group and vehicle treated group indicated that this result was statistically significant (P < 0.01). P- values were calculated using an ANOVA analysis. See, e.g., Mathematical Statistics and Data Analysis, 1988, Wadsworth & Brooks, Pacific Grove, CA. This analysis demonstrated that FGFR1-ECD.339-Fc significantly reduced tumor growth in the PDX lung tumor model D35087, which does not have amplification of the FGFR1 gene, but expresses relatively high-levels of FGFR1 mRNA.

[0231] Thus, certain lung cancer xenograft models that do not have FGFR1 gene amplification, but which have FGFR1 overexpression, are sensitive to FGFR1-ECD.339-Fc treatment.

Example 8: Predictors of FGFR1-ECD.339-Fc response

[0232] The RNA expression of a panel of FGFR1 -related genes including FGF ligands, FGF receptors, FGF binding proteins, FGF signaling molecules, and a group of angiogenesis- related targets was determined in a set of 35 tumor cell lines and xenografts using qRT-PCR. RNA was extracted from cell lines grown in vitro or tumor xenografts grown in vivo using the RNAeasy® mini kit (Qiagen, Germany). Extracted RNA was treated with DNAse I prior to creating cDNA with random hexamer priming and reverse transcriptase using the

QuantiTect Reverse Transcription Kit (Qiagen, Germany). Human and mouse RNA expression was determined using QuantiTect Primer Assays (Qiagen, Germany) employing a human GUSB control reference QuantiTect Primer Assay (Qiagen, Germany). QuantiTect SYBR Green PCR Kits (Qiagen, Germany) were used to quantify mRNA expression levels using real-time qRT-PCR and an ABI Prism ViiA™ 7 Real-Time PCR System (Applied Biosystems, Foster City, CA). Relative gene expression quantification was calculated according to the comparative Ct method using human GUSB as a reference and commercial RNA controls (Stratagene, La Jolla, CA). Relative quantification was determined according to the formula: ₂-(^{ACt sam}P^le-^{ACt calibratOT}).

[0233] The tumor cell lines and xenografts used in this experiment are shown in Table 5. Also shown in Table 5 are the dosing schedule for FGFR1-ECD.339-Fc in a mouse xenograft model, the percent tumor growth inhibition (TGI (%)) and the statistical significance of the tumor growth inhibition (P Value), as well as whether the FGFR1 gene is amplified in the cell line.

Table 5: Anti-tumor activity of FGFR1-ECD.339-Fc in a panel of xenograft models

amplified

Non-

D37638 PDX IP 15 mg/kg BIW 0% ns amplified

Non-

D35376 PDX IP 15 mg/kg BIW 0% ns amplified

Non-

LXFA-737 PDX IP 15 mg/kg BIW 0% ns amplified

Non-

LXFA-629 PDX IP 15 mg/kg BIW 65% P = 0.007 amplified

MesothelMSTO- P < Non- ioma 211H Cell Line IP 15 mg/kg BIW 64% 0.0001 amplified

Non-

U-87 Cell Line IP 15 mg/kg BIW 0% ns amplified

GlioNon- blastoma U-118 Cell Line IP 15 mg/kg BIW 36% ns amplified

P = Non-

U-251 Cell Line IP 15 mg/kg BIW 48% 0.0078 amplified

RetinoNon- blastoma Y79 Cell Line IP 10 mg/kg BIW 0% ns amplified

0.15 Non-

Prostate Dul45 Cell Line IP mg/kg 3x/w 31% ns amplified

Non-

MFE-280 Cell Line IP 15 mg/kg BIW 96% P < 0.001 amplified

EndoNon- metrial HEC-1B Cell Line IP 15 mg/kg BIW 30% < 0.05 amplified

Non-

MFE-319 Cell Line IP 15 mg/kg BIW 0% ns amplified

MDA-MB- Non- 231 Cell Line IP 15 mg/kg BIW 0% ns amplified

Breast

Non-

JIMT1 Cell Line IP 1 mg/kg BIW 28% < 0.05 amplified

[0234] An exemplary xenograft experiment is as follows. For Caki-1 and MSTO-21 1H, five million cells were implanted subcutaneously over the right flank of SCID mice (N=10 per group). FGFR1-ECD.339-Fc or albumin was administered i.p. twice a week at the dose indicated in Table 5. FIG. 16 shows anti-tumor activity of FGFR1-ECD.339-Fc in selected xenograft models. Representative tumor growth curves are shown for a renal cancer, Caki-1, (A), and mesothelioma, MSTO-211H, (B) xenograft cancer model. In the renal cell carcinoma (RCC) Caki-1 model, administration of FGFR1-ECD.339-Fc at 10 mg/kg twice a week for 6 weeks resulted in 81% (P < 0.001) tumor growth inhibition (TGI; FIG. 16a). In the MSTO-211H mesothelioma model, FGFR1-ECD.339-Fc administration reduced tumor growth (FIG. 16b) by 64% (P < 0.0001). In responding tumors, FGFR1-ECD.339-Fc significantly reduced tumor volume as assessed by area-under-the-curve (AUC) analysis. Responses were observed in 19/35 (54 %) of the models examined, with a range of 25-96% inhibition (see Table 5). [0235] In order to further understand the potential molecular determinants that make xenograft models sensitive to treatment with FGFR1-ECD.339-Fc, the RNA expression of a panel of genes including FGF ligands, FGF receptors, FGF binding proteins and FGF signaling molecules was examined using qRT-PCR in certain xenograft models from Table 5. The results are shown in Table 7, below.

[0236] Gene expression was then correlated to FGFR1-ECD.339-Fc response to determine RNA expression signatures positively and negatively correlated with anti-tumor activity. Table 8 shows the results of that analysis. In addition to FGF2, RNA expression of FGF 18 (P = 0.02227) was also positively (6.9-fold) correlated with FGFR1-ECD.339-Fc anti-tumor activity. The downstream target gene of FGF signaling, ets variant 4 (ETV4), was the most significant (P = 0.01639) gene for its positive (2.897-fold) association with FGFR1- ECD.339-Fc activity. Expression oiFGFRl (P = 0.01276), including the FGFRlIIIc splice variant (P = 0.01603), was a positive predictor for FGFR1-ECD.339-Fc response. Expression of the FGFRlIIIb splice variant was not correlated with FGFR1-ECD.339-Fc response in that experiment. In addition to FGFR1, expression of the FGFR3IIIc receptor (P = 0.02488) was also positively correlated with FGFR1-ECD.339-Fc response, reflecting the potential overlap in FGF-ligand binding affinities between the Illc-splice isoforms of FGFR1 and FGFR3 receptors. Significant genes with a negative association with FGFR1-ECD.339-Fc activity were not found in this analysis.

Table 8: Statistical analysis of FGF-related gene expression in relation to FGFR1- ECD.339-Fc anti-tumor response in xenograft models

^Gene expression ratio determined by median gene expression in FGFR1-ECD.339-Fc responders / non-responders

^† -values are determined by a Mann- Whitney test of PCR gene expression in responders vs. non-responders for each gene using all models in Table 5.

[0237] To determine what RNA factors may determine lung xenograft response in the absence of FGFRl-gene amplification, the correlation of FGFR1-ECD.339-Fc response in the non-FGFRl amplified subset of lung models was examined (N = 13). The results of that analysis are shown in Table 9. FGF2 expression was up-regulated >3,000 fold in responding vs. non-responding FGFR1 non-amplified lung models (P = 0.029). The expression of

FGFRlIIIc and FGFR3IIIc also displayed a positive trend with FGFR1-ECD.339-Fc response in the non-FGFRl amplified lung subset in this experiment.

Table 9: Statistical analysis of FGF-related gene expression in relation to FGFR1- ECD.339-Fc anti-tumor response in non-FGFRl amplified lung xenograft models

^Gene expression ratio determined by median gene expression in FGFR1-ECD.339-Fc responders /median gene expression in non-responders

^† -values are determined by a Mann- Whitney test of PCR gene expression in responders vs. non-responders for each gene using the non-FGFRl amplified lung models in table 5.

[0238] It was examined if there was a correlation in gene expression amongst the significant gene markers identified for their association with FGFR1-ECD.339-Fc response in all models. The results of that analysis are shown in Table 10. In this experiment, there was a significant, positive correlation between the majority of the individual RNA markers identified as predictive for FGFR1-ECD.339-Fc xenograft response. For example, xenograft

FGF2 RNA expression is positively correlated with FGFR3IIIc, FGFRlIIIc and FGFR1 expression (P < 0.05); FGFR1 RNA expression is positively correlated with FGFR3IIIc,

FGF2 and FGF18. The expression of ETV4 was not associated with other FGFR1-ECD.339-

Fc responsive genes.

Table 10: Spearman correlation of gene expression markers predictive of FGFR1-

FGF18 FGFRlIIIc 0.57 0.0008

FGF2 FGFR3IIIC 0.49 0.0139

FGFR1 FGFR3IIIC 0.41 0.0244

FGF2 FGFRlIIIc 0.43 0.0336

FGF2 FGFR1 0.39 0.0447

^¾2-sided p-values approximated with a Monte Carlo simulation

[0239] FIG. 14 shows (A) FGF2 mRNA (normalized to GUSB) and (B) FGF2 protein expression in FGFR1-ECD.339-Fc responder and non-responder xenografts. Expression of FGF2 (P = 0.03569) was positively associated with FGFR1-ECD.339-Fc response. FGF2 displayed a high ratio (247.7-fold) of mRNA gene expression between FGFR1-ECD.339-Fc responder and non-responder xenografts. FGF2 protein levels were also confirmed to correlate with FGFR1-ECD.339-Fc response.

[0240] FIG. 17 shows (A) FGFR1 mRNA expression (normalized to GUSB) and (B) FGFR3IIIC mRNA expression (normalized to GUSB) in FGFR1-ECD.339-Fc responder and non-responder xenografts. Expression of FGFR1 (P = 0.01669; Fig 17a), and the FGFRlIIIc splice variant (P = 0.0431 ; Table 8), was positively correlated with FGFR1-ECD.339-Fc antitumor activity. In addition to FGFR1, expression of the FGFR3IIIc receptor (P = 0.01944, Table 8) was also positively correlated with FGFR1-ECD.339-Fc anti-tumor response (FIG. 5b), reflecting the overlap in FGF-ligand binding specificity between the c-splice isoforms of FGFR1 and FGFR3 receptors (see, e.g., Zhang, et al. J. Biol. Chem. 281, 15694-15700 (2006); Ornitz, et al. J. Biol. Chem. 271, 15292-15297 (1996)).

Example 9: Predictor of FGFR1-ECD.339-Fc response

[0241] DKK3 mRNA expression was determined in a set of 25 xenografts using qRT- PCR. RNA was extracted from tumor xenografts grown in vivo using the RNAeasy® mini kit (Qiagen, Germany). Extracted RNA was treated with DNAse I prior to creating cDNA with random hexamer priming and reverse transcriptase using the QuantiTect Reverse Transcription Kit (Qiagen, Germany). Human DKK3 RNA expression was determined using QuantiTect Primer Assays (Qiagen, Germany) employing a human GUSB control reference QuantiTect Primer Assay (Qiagen, Germany). QuantiTect SYBR Green PCR Kits (Qiagen, Germany) were used to quantify mRNA expression levels using real-time qRT-PCR and an ABI Prism ViiA™ 7 Real-Time PCR System (Applied Biosystems, Foster City, CA).

Relative gene expression quantification was calculated according to the comparative Ct method using human GUSB as a reference and commercial RNA controls (Stratagene, La Jolla, CA). Relative quantification was determined according to the formula: 2 ^{samp e"} calibrator)

[0242] The tumor xenografts used in this experiment are shown in Table 1 1. Also shown in Table 1 1 are the dosing schedule for FGFR1-ECD.339-Fc in a mouse xenograft model, the percent tumor growth inhibition (TGI (%)) and the statistical significance of the tumor growth inhibition (P Value).

Table 11. Panel of xenograft models with microarray data.

[0243] Gene expression was then correlated to FGFR1-ECD.339-Fc response to determine RNA expression signatures positively and negatively correlated with anti-tumor activity. Expression of DKK3 mRNA was higher in tumors that were sensitive to FGFR1-ECD.339- Fc than in tumors that were not sensitive to FGFR1-ECD.339-Fc (P = 0.0069). [0244] FIG. 15 shows DKK3 mR A levels (normalized to GUSB) in FGFR1-ECD.339-

Fc responder and non-responder xenografts. The horizontal line indicates the median expression level for that group.

Example 10: FGFR1-ECD.339-Fc does not increase serum phosphate following high dose administration in rats

[0245] FGFR 1 -ECD .339 -Fc binds to the mitogenic FGFs with 10 to 100-fold higher affinity than to FGF-23. The binding affinity of FGFR1-ECD.339-Fc for rodent FGF-23 is comparable to that of human FGF-23 by SPR analysis (6.0 x 10^"8 vs. 6.7 x 10^"8 M). The potential biological impact of this relatively weak FGFR1-ECD.339-Fc /FGF-23 binding was investigated in rats following four weekly doses of FGFR1-ECD.339-Fc at a dose range of

10-200 mg/kg/qwk.

[0246] In the first experiment, Sprague Dawley rats (Charles River Labs; N = 5/group) were dosed with vehicle, 10, 50 or 200 mg/kg/qwk of FGFR1-ECD.339-Fc for four weekly doses and plasma concentrations of FGFR1-ECD.339-Fc were determined throughout the study by an ELISA based detection method.

[0247] FGFR1-ECD.339-Fc concentration in plasma was determined using a quantitative ELISA. Briefly, recombinant human FGF-2 (R&D Systems) was immobilized on a half-well microtiter ELISA plate, blocked and incubated with test samples (diluted 1 : 10 with blocking buffer/20 μg/mL of heparin). The plate was subsequently washed and a dilute goat anti- human IgG-Fc HRP antibody solution (Sigma) was added and incubated. After a final wash step, a tetramethylbenzidine peroxidase substrate solution was added and incubated at ambient temperature with gentle shaking. The reaction was stopped with a phosphoric acid solution. Plates were read on a plate reader (450 nm). FGFR1-ECD.339-Fc concentrations were determined on a standard curve obtained by plotting optical density (OD) versus concentration.

[0248] In the second experiment, Sprague Dawley rats (Charles River Labs; N = 5/group) were administered the FGFR kinase inhibitor PD 173074 (Chemdea, Ridgewood, NJ; 50 mg/kg/day) or vehicle control by oral gavage for 7 days; or were administered FGFR1- ECD.339-Fc (200 mg/kg) or appropriate vehicle weekly by intravenous administration. Blood samples were collected at the time points indicated and serum phosphate was determined at 24 and 168 hours post-initiation of dosing (Idexx laboratories, Westbrook, MA).

[0249] The results of those experiments are shown in FIG. 18. At the 200 mg/kg/qwk dose the maximal plasma concentration of the drug was 3.6 and 4.2 mg/ml for female and male rats, respectively (FIG. 18A). Despite these sustained high levels of drug, no significant changes in plasma phosphate were observed for any FGFR1-ECD.339-Fc dose compared to animals that received vehicle (9.61 vs. 10.19 mg/dL for vehicle and 200 mg/kg/qwk FGFR1-

ECD.339-Fc, respectively). In contrast, daily dosing of rats with the small molecule FGFR kinase inhibitor PD 173043 resulted in significantly elevated plasma phosphate levels either at

24 hour or 1 week of daily dosing (FIG. 18B). Additionally, histological analysis of 55 tissues in animnals treated with high-dose FGFR1-ECD.339-Fc failed to reveal any changes consistent with those reported by Brown et al. (Toxicol. Pathol. 33, 449-455 (2005)), who observed hyperphosphatemia and calcium-phosphorus deposition in various organs following administration of a small molecule inhibitor of FGFR1 kinase activity.

[0250] In addition, FGFR1-ECD.339-Fc has completed a phase 1 dose-escalation study

(N=39) of up to 16 mg/kg/qwk in patients with solid tumors. No impact of FGFR1-ECD.339-

Fc on serum phosphate was observed at any of the dose-levels examined (See, e.g., Tolcher, et al. Proceedings of the 22nd EORTC-NCI-AACR Symposium on Molecular Targets and

Cancer Therapeutics (2010)). In summary, these results support the biophysical data that

FGFR1-ECD.339-Fc does not bind to FGF-23 with high-affinity and does not induce hyperphosphatemia as was shown for other broad inhibitors of the FGFR pathway.

Example 11: FGFR1-ECD.339-Fc mediated inhibition of FGF-2 and VEGF-A induced angiogenesis in a matrigel plug assay

[0251] Recombinant human FGF-2 (final concentration 250 ng/ml; Peprotech) and/or recombinant human VEGF-A (final concentration 100 ng/ml; Peprotech) were added to matrigel (BD Biosciences, Franklin Lakes, NJ) with sodium heparin (2 units/ml; Sigma).

FGF-2 and/or VEGF-A containing matrigel plugs (one per animal) were implanted subcutaneous ly in the abdomen region of C57BL/6 mice (Charles River, Wilmington, MA).

FGFR1-ECD.339-Fc was administered by tail vein injection on days 1, 4, and 7 post-matrigel implantation. On day 9, plugs were excised and processed for hematoxylin and eosin (H&E) staining. Digital images of the stained matrigel sections were generated using a Retiga 2000R digital camera (Qlmaging, Burnaby, BC). Image analysis was performed using Image-Pro

Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD). Neovascularization was defined as the cellular response in the Matrigel plugs, consisting of newly formed blood vessels and migrated cells.

[0252] The results of that experiment are shown in FIG. 19. Administration of 5 mg/kg or higher FGFR1-ECD.339-Fc completely blocked in vivo angiogenesis induced by a matrigel plug impregnated with FGF-2. Administration of 15 or 45 mg/kg FGFR1-ECD.339-Fc also completely blocked in vivo angiogenesis in response to a matrigel plug impregnated with VEGF-A only or FGF-2 plus VEGF-A. Anti-angiogenic activity against VEGF induced angiogenesis in this model system may reflect inhibition of the synergistic activity between VEGF in the plug and murine-derived stromal FGFs since SPR analysis shows that FGFR1- ECD.339-Fc does not directly interact with VEGF-A.

[0253] To determine whether FGFR1-ECD.339-Fc blocks VEGF-induced proliferation of endothelial cells, HUVEC cells (Life Technologies, Grand Island, NY) were seeded at a density of 4X10³ cells/well in basal media (Medium 200 (Life Technologies) with 2% heat inactivated FBS) and stimulated with either 10 ng/ml FGF2 (R&D Systems, Minneapolis,

MN) or 15 ng/ml VEGF-A 165 (R&D Systems, Minneapolis, MN) either in the presence of absence of 10 μg/ml FGFR1-ECD.339-Fc. HUVEC cell proliferation was determined 3 days post-stimulation using CellTiter-Glo® Luminescent Cell Viability Assay.

[0254] The results of that experiment are shown in FIG. 20. FGFR1-ECD.339-Fc did not block VEGF-induced proliferation of HUVECs, although it is capable of blocking FGF-2 induced HUVEC proliferation.

Example 12: FGFR1-ECD.339-Fc inhibits tumor angiogenesis in the Caki-1 renal cell carcinoma xenograft model

[0255] Human renal carcinoma Caki-1 cells (1.5 x 10⁷ cells/mouse) cells were implanted subcutaneous ly into the right flank of CB17-SCID mice. One day after tumor implantation, the mice were randomized and treated intravenously with either vehicle or FGFR1-ECD.339-

Fc (5 mg/kg) twice a week. At the end of the study (Day 57), tumors were excised (N=3/gp) and used for histological analysis. Frozen sections were probed with anti-mouse CD31 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ) and visualized using HRP- conjugated conjugated secondary antibody coupled with diaminobenzidine staining (brown color). Slides were counter-stained with hematoxylin to identify cell nuclei (blue color).

Representative sections are shown (5x magnification).

[0256] The results of that experiment are shown in FIG. 21. Following treatment with FGFR1-ECD.339-Fc, reduced CD31 staining is observed, indicatingthat tumor angiogenesis was inhibited by FGFR1-ECD.339-Fc administration in this experiment. Example 13: FGFR1-ECD.339-Fc -mediated inhibition of FGFR1 signaling in the JIMT-1 breast cancer xenograft model

[0257] Animals with established (200mm3) human breast cancer JIMT-1 tumors were administered either a single (24 and 72 hour timepoints) or three times per week (multidose) i.p. dose(s) of FGFR1-ECD.339-Fc at 15 mg/kg. Tumor samples were collected at 24 and 72 hours post-dose for the single dose groups and 48 hours post the last dose in multi-dose group, snap-frozen in liquid nitrogen and lyzed in RIPA buffer (Sigma Aldrich, St Luis, MO).

Tumor lysates were separated by SDS-PAGE and western blotting was performed using monoclonal antibodies FGFR1, pFGFRl, FRS2a, pFRS2a, Akt, pAkt, and Actin (Cell

Signaling Technology, Inc). FGFR1-ECD.339-Fc was detected using anti-human Fc monoclonal antibody (Jackson Immuno Research).

[0258] The results of that experiment are shown in FIG. 22. FGFR1-ECD.339-Fc reduced levels of phosphorylated FGFR1 by 24 hours post-dose and completely abolished FGFR1 phosphorylation by 72 hours post-dose. Phosphorylated FRS and Akt levels were reduced 24 hours post-dose and further reduced two days later. Thus, FGFR1-ECD.339-Fc inhibited FGFR1 signaling in the JIMT-1 breast cancer xenograft model.

TABLE OF SEQUENCES

[0259] Table 6 lists certain sequences discussed herein. FGFRl sequences are shown without the signal peptide, unless otherwise indicated.

Table 6: Sequences and Descriptions

SEQ ID NO Description Sequence

MWSWKCLLFW AVLVTATLCT ARPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD

Full-length human ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME FGFRl ECD (with KKLHAVPAAK TVKFKCPSSG TPNPTLRWLK NGKEFKPDHR signal peptide); SP- IGGYKVRYAT WSIIMDSWP SDKGNYTCIV ENEYGSINHT hFGFRl-ECD.353 YQLDWERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD

PQPHIQWLKH IEV GSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL EERPAVMTSP LYLE

RPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA

Full-length human CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE FGFRl ECD (without TDNTKPNPVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG signal peptide); TPNPTLRWLK NGKEFKPDHR IGGYKVRYAT WSIIMDSWP

SDKGNYTCIV ENEYGSINHT YQLDWERSP HRPILQAGLP

hFGFRl-ECD.353

ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL EERPAVMTSP LYLE

MWSWKCLLFW AVLVTATLCT ARPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME

SP-hFGFRl-ECD.339 KKLHAVPAAK TVKFKCPSSG TPNPTLRWLK NGKEFKPDHR

IGGYKVRYAT WSIIMDSWP SDKGNYTCIV ENEYGSINHT YQLDWERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL

RPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG

hFGFRl-ECD.339 TPNPTLRWLK NGKEFKPDHR IGGYKVRYAT WSIIMDSWP

SDKGNYTCIV ENEYGSINHT YQLDWERSP HRPILQAGLP

ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL

MWSWKCLLFW AVLVTATLCT ARPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE TDNTKPNPVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG TPNPTLRWLK NGKEFKPDHR

SP-hFGFRl-ECD.339- IGGYKVRYAT WSIIMDSWP SDKGNYTCIV ENEYGSINHT Fc YQLDWERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD

PQPHIQWLKH IEVNGSKIGP DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL AGNSIGLSHH SAWLTVLEAL EPKSSDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVWD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRWSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP G

RPSPTLPEQ AQPWGAPVEV ESFLVHPGDL LQLRCRLRDD VQSINWLRDG VQLAESNRTR ITGEEVEVQD SVPADSGLYA CVTSSPSGSD TTYFSVNVSD ALPSSEDDDD DDDSSSEEKE TDNTKPNFVA PYWTSPEKME KKLHAVPAAK TVKFKCPSSG TPNPTLRWLK NGKEFKPDHR IGGYKVRYAT WSIIMDSWP SDKGNYTCIV ENEYGSINHT YQLDWERSP HRPILQAGLP ANKTVALGSN VEFMCKVYSD PQPHIQWLKH IEVNGSKIGP

hFGFRl-ECD.339-Fc DNLPYVQILK TAGVNTTDKE MEVLHLRNVS FEDAGEYTCL

AGNSIGLSHH SAWLTVLEAL EPKSSDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVWD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRWSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

hFGFRl signal peptide MWSW CLLFWAVLVTATLCTA

EPKSSDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVWD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRWSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT

Fc C237S ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS

DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK

ERKCCVECPP CPAPPVAGPS VFLFPPKPKD TLMISRTPEV TCVWDVSHE DPEVQFNWYV DGVEVHNAKT KPREEQFNST FRWSVLTW HQDWLNGKEY KCKVSNKGLP APIEKTISKT

Exemplary Fc #1 KGQPREPQVY TLPPSREEMT KNQVSLTCLV KGFYPSDIAV

EWESNGQPEN NYKTTPPMLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGK

ESKYGPPCPS CPAPEFLGGP SVFLFPPKPK DTLMISRTPE VTCVWDVSQ EDPEVQFNWY VDGVEVHNAK TKPREEQFNS TYRWSVLTV LHQDWLNGKE YKCKVSNKGL PSSIEKTISK

Exemplary Fc #2 AKGQPREPQV YTLPPSQEEM TKNQVSLTCL VKGFYPSDIA

VEWESNGQPE NNYKTTPPVL DSDGSFFLYS RLTVDKSRWQ EGNVFSCSVM HEALHNHYTQ KSLSLSLGK

Table 7. Gene expression values for certain xenograft models from Table 5.

Gene A498 A549 Caki-1 Calu-1 Colo201 Colo205 D35087

FGFBP3 0.000203 0.001861 0.003217 0.000868 0.001642 0.002438 NA

FGFR1 0.356012 0.535887 1.1487 1.53688 0.664343 0.126745 4.30765

FGFRlIIIb 0.000152 0.000309 0.000288 0.000282 0.000963 0.000456 NA

FGFRlIIIc 0.119908 0.131215 0.193446 0.646176 0.114229 0.009753 0.381142

FGFR2 0.166086 0.001186 0.00072 0.001554 0.092142 0.003401 2.3227

FGFR2IIIb 0.009163 0.000334 8.63E-05 0.000169 0.045753 0.001797 NA

FGFR2IIIC 0.196146 0.000175 0.000133 0.000804 0.000275 8.51E-05 0.00162

FGFR3 0.327598 0.044811 0.456916 0.033493 0.148651 0.038741 4.50554

FGFR3IIIb 0.006661 0.006003 0.006524 0.00014 0.023036 0.010167 NA

FGFR3IIIC 0.039555 0.001576 0.063813 0.005048 0 0 0.001059

FGFR4 0.167241 0.111105 0.558644 0.000399 0.184284 0.107321 0.041146

FLRT1 0.002489 0.02352 0.01209 0.007867 0.040107 0.076415 NA

FLRT2 4.03E-05 0.042986 0.003879 1.12506 0 5.24E-05 NA

FLRT3 0.001586 0.051474 0.042986 0.000052 0.000186 0.001773 NA

HGF 0 0.007977 0.033961 0.000725 0 0 0

IGF1 0.000405 0.002613 0 0.000381 3.25E-05 0 NA

IGF1R 0.02977 0.598739 0.071794 0.469761 1.10957 1.01396 NA

IGF2 0.004129 0.05954 0.060371 0.043285 0.002438 0.000299 NA

KDR 0.000502 8.34E-05 0.000238 0.01418 0.000478 0.000122 0.000281

MET 1.28343 0.503478 7.26015 1.50525 0.790041 0.366021 NA

MMP1 2.51E-05 0.018841 0.007599 0.303549 0.000413 0.000899 NA

MMP2 1.54E-05 0.030186 0.888843 2.39496 0 0 12.3138

NCAM1 0.05366 5.85E-05 0.000485 0.000394 0.000159 2.44E-05 NA

PDGFRa 0.000627 0.00095 0.173139 0.219151 0 0 0.023016

PDGFRb 0.001887 0.000735 0.021793 0.952638 0.002405 0.001114 NA

PLAU 0.013888 0.267943 5.20537 0.456916 0.271684 0.289172 NA

PLAUR 0.228458 0.97942 0.920188 1.94531 0.582367 0.248273 NA

SERPINE1 0.61132 0.230047 1.94531 9.00047 0.077482 0.105843 NA

SOX9 0.602904 1.26576 2.82843 1.72907 1.87905 4.85678 NA

SPRY1 0.013415 0.022718 0.160428 0.198884 0.119908 0.186856 NA

SPRY2 0.028756 0.136787 0.5 0.301452 0.395021 0.50698 NA

SPRY3 0.002668 0.003086 0.014579 0.001491 0.002668 0.003521 0.003134

SPRY4 0.002372 0.001565 0.005336 0.022876 0.009163 0.020905 NA

TGFa 0.456916 0.051833 0.258816 0.009552 0.271684 0.127626 NA

TNC 0.002542 0.007139 0.222211 1.67018 0.50698 0.123279 NA

VIM 27.0958 13.8326 122.786 60.9688 0.336808 0.166086 43.9259 Gene D35376 D37638 DMS114 DMS53 Dul45 G-401 HCT116

AREG 0.004051 1.51362 0.000292 0.008144 0.166086 0.0019 2.18859

CA12 NA NA NA NA 0.015303 0.02936 0.026278

CDH1 NA NA NA NA 0.933033 0.003262 1.09429

DKK3 0.000737 3.12315 NA NA 0.010237 0.018073 5.43E-05

DUSP4 NA NA NA NA 0.01468 0.000155 0.052193

DUSP5 NA NA NA NA 0.028956 0.011281 0.316439

DUSP6 NA NA NA NA 0.692555 2.63902 4.08405

EGF NA NA 0 0.000918 0.065607 1.09E-05 0.008609

EGFR NA NA NA NA 0.594604 0.000399 1.42405

ELK3 NA NA NA NA 0.041521 0.156041 0.234881

ELK4 NA NA NA NA 0.000023 6.28E-05 0.000104

ERBB2 NA NA NA NA 0.389582 0.121582 0.217638

ERBB3 0.000903 0.108909 0.001913 0.012691 0.260616 0.031686 0.231647

EREG 0 0.002591 0 9.93E-06 0.034197 0.003853 5.65685

ETV4 0.151082 1.54928 NA NA 0.014579 1.20581 0.15822

ETV5 NA NA NA NA 0.046071 0.426317 0.371131

FGF1 0.000328 0.050036 NA NA 0.001631 0.000176 0.034674

FGF10 0.000157 0.00023 NA NA 3.39E-05 0.5 0.000192

FGF11 0.012728 0.101173 NA NA 0.008669 0.251739 0.022876

FGF16 0.026669 0.026479 NA NA 0.000585 0.000311 0.000918

FGF17 0.000632 0.006306 NA NA 0.006801 0.000681 0.011359

FGF18 0.000445 0.002484 NA NA 0.00286 0.003826 0.03082

FGF19 NA NA NA NA 0.000128 0.000937 0.035897

FGF2 NA NA NA NA 0.107321 0.008373 0.10083

FGF20 NA NA NA NA 0.00145 0.30566 0.00613

FGF21 NA NA NA NA 0.000193 4.59E-05 0.000231

FGF22 NA NA NA NA 0.008373 0.002668 0.01937

FGF3 NA NA NA NA 0 6.23E-05 0.000331

FGF4 NA NA NA NA 0 0 0

FGF5 5.91E-05 0.000808 NA NA 0 1.84E-05 0

FGF6 NA NA NA NA 0.000052 0 0.00015

FGF7 0 0 NA NA 7.11E-05 0.000233 0.000045

FGF8 0.000961 0.001714 NA NA 0.000301 0.01541 0.006003

FGF9 NA NA NA NA 0.003065 0.001137 0.009227

FGFBP1 NA NA NA NA 0.050067 0 0.248273

FGFBP2 NA NA NA NA 0.001211 0.00029 0.005048 Gene D35376 D37638 DMS114 DMS53 Dul45 G-401 HCT116

FGFBP3 NA NA NA NA 0.000618 0.060371 0.00588

FGFR1 0.581641 0.709808 0.678302 0.078563 0.220676 1.32869 0.517632

FGFRlIIIb NA NA 0 0 0.001665 5.62E-05 0.085378

FGFRlIIIc 0.069464 0.386462 0.027585 0.01698 0.057512 0.473029 0.063373

FGFR2 0.000917 1.05416 0.008974 0.001084 0.033032 1.22264 0.137738

FGFR2IIIb NA NA NA NA 0.023036 0.049721 0.118257

FGFR2IIIC 0.000498 0.012137 NA NA 0.00075 0.972655 0.000294

FGFR3 0.009346 0.580312 0.009163 0.002093 0.033262 0.025559 0.329877

FGFR3IIIb NA NA NA NA 0.005799 0.000844 0.030607

FGFR3IIIC 9.87E-05 0.00035 NA NA 0.000135 0.003747 6.36E-05

FGFR4 0.000564 0.009061 0.002879 0.000168 0.004395 0.015953 0.042394

FLRT1 NA NA NA NA 0.01698 0.005839 0.034197

FLRT2 NA NA NA NA 0.009889 0.010027 0

FLRT3 NA NA NA NA 0.007867 0.000886 0.002372

HGF 0.044508 0.009057 NA NA 6.2E-06 2.23457 0

IGF1 NA NA NA NA 0.002036 0.000294 0

IGF1R NA NA NA NA 0.297302 0.065154 0.088388

IGF2 NA NA NA NA 0.006754 0.104386 0.20166

KDR 0.000377 0.009784 NA NA 0.00294 0.000142 0.000557

MET NA NA NA NA 0.119908 0.003747 1.1487

MMP1 NA NA NA NA 0.044502 0.000184 0.002339

MMP2 0.000158 0.138658 NA NA 0 0.325336 0

NCAM1 NA NA NA NA 0.000061 0.562529 0.003401

PDGFRa 0.005323 0.038353 NA NA 0.000208 0.001748 0

PDGFRb NA NA NA NA 0.001381 0.007443 0.00294

PLAU NA NA NA NA 0.289172 0.00324 0.297302

PLAUR NA NA NA NA 0.194791 0.035403 0.429283

SERPINE1 NA NA NA NA 0.03983 0.001153 0.45376

SOX9 NA NA NA NA 0.063813 0.012174 1.94531

SPRY1 NA NA NA NA 0.004876 0.088388 0.030396

SPRY2 NA NA NA NA 0.027017 0.721965 0.055553

SPRY3 0.00269 0.006099 7.89E-05 0.000644 0.007599 0.007922 0.020054

SPRY4 NA NA NA NA 0.000162 0.00162 0.003773

TGFa NA NA NA NA 0.05954 0.000428 0.121582

TNC NA NA NA NA 0.014579 0.000162 0.000118

VIM 16.4293 3.26549 NA NA 2.15846 38.5858 0.051119 LXFA- MDA- MFE- MFE-

Gene HEC-1B JIMT1 LXFA-629 737 MB-231 280 319

AREG 0.000804 0.0625 0.794269 0.941087 1.37554 0.001511 0.001271

CA12 2.8481 0.010672 NA NA 0.119908 0.02683 0.035403

CDH1 0.033493 3.20428 NA NA 0.000139 0.602904 0.895025

DKK3 0.646176 0.118257 0.039949 0.067093 0.000516 0.188156 0.000761

DUSP4 0.000446 0.023683 NA NA 0.070805 0.001511 6.87E-05

DUSP5 0.203063 0.050067 NA NA 0.432269 0.039282 0.02936

DUSP6 2.36199 0.183011 NA NA 3.68075 1.3566 0.084202

EGF 0.00588 0.023196 NA NA 0.011125 0.001061 0.00362

EGFR 0.432269 3.03143 NA NA 1.86607 0.092783 0.307786

ELK3 0.628507 0.154963 NA NA 0.539614 0.03983 0.037163

ELK4 0.000032 0.00143 NA NA 8.28E-05 0 8.3E-06

ERBB2 0.535887 5.06303 NA NA 0.11744 1.31039 0.48971

ERBB3 0.072293 0.271684 0.152936 1.94598 0.046071 0.309927 0.080214

EREG 2.08E-05 0.06164 0.067803 0.041083 0.25349 1.78E-05 0.000119

ETV4 0.528509 0.493116 0.185141 0.889459 0.210224 0.888843 0.011598

ETV5 0.371131 0.179244 NA NA 0.248273 0.05672 0.017824

FGF1 0.003354 0.036398 0.0984 0.004799 0.077482 0.000462 0.001032

FGF10 3.03E-05 0 3.2E-05 2.51E-05 3.23E-05 0.000168 4.9E-06

FGF11 0.009552 0.017948 0.173307 0.554631 0.003086 0.057115 0.009037

FGF16 9.78E-05 0.002137 0.016327 0.025879 0.000341 0.000485 0.000147

FGF17 0.000821 0.024349 0.000633 0.003234 0.000391 0.034197 0.013139

FGF18 1.45397 0.057115 0.00032 0.001085 0.001362 0.049378 0.043586

FGF19 0 0 NA NA 7.26E-05 0.008432 7.3E-06

FGF2 0.021793 0 NA NA 0 0.009889 0.001598

FGF20 0.006896 0 NA NA 0.001785 0.001004 0.000016

FGF21 2.66E-05 0.000452 NA NA 1.25E-05 0.000084 7.1E-06

FGF22 0.00519 0.019237 NA NA 0.003401 0.012691 0.049037

FGF3 0.000011 0.001289 NA NA 0 0.000735 0

FGF4 0 0 NA NA 0 0.000437 0

FGF5 0.001011 0.004364 0.006428 5.47E-05 0.181747 2.23E-05 7.8E-06

FGF6 0 0 NA NA 9.5E-06 0 3.09E-05

FGF7 2.14E-05 0 0 0 4.14E-05 9.85E-05 0.003173

FGF8 0.001325 0.00064 7.08E-05 0.000522 8.11E-05 0.000331 0.000368

FGF9 0.001011 0.008549 NA NA 0.000495 0.001245 0.013697

FGFBP1 0.20733 0.664343 NA NA 0.002244 0.002355 0.002065

FGFBP2 0.003195 0.000428 NA NA 0.000127 0.001887 0.003961 LXFA- LXFA- MDA-MB- MFE- MFE-

Gene HEC-1B JIMT1 629 737 231 280 319

FGFBP3 0.000267 0.003065 NA NA 0.00734 0.001047 0.00162

FGFR1 0.479632 5.89708 0.6208 0.448755 0.524858 1.22264 0.554785

FGFRlIIIb 0.000475 0.204476 NA NA 0.00097 0.00734 0.000509

FGFRlIIIc 0.236514 1.86607 0.114633 0.108525 0.204476 1.02101 0.267943

FGFR2 0.050067 1.21419 0.121945 0.001513 0.003065 0.027394 0.211686

FGFR2IIIb 0.012344 0.602904 NA NA 0.001169 0.014279 0.160428

FGFR2IIIC 0.016289 0.005448 2.79E-05 0.000266 0.000137 0.001178 0.009486

FGFR3 0.200267 0.840896 1.05256 1.51215 0.005154 0.094732 0.062935

FGFR3IIIb 0.023196 0.148651 NA NA 0.00147 0.007391 0.005486

FGFR3IIIC 0.013139 0.000194 0.000669 0.000864 0.000132 0.000144 0.000152

FGFR4 0.225313 0.094732 0.005931 0.111491 0.000523 0.013985 0.004581

FLRT1 0.00362 0.018711 NA NA 0.031034 0.041521 0.040667

FLRT2 0.001677 0 NA NA 0.069348 0.00362 0.089003

FLRT3 0.041521 0 NA NA 2.87E-05 0.002228 0.034197

HGF 2.62E-05 0 4.75E-05 0 0 5.13E-05 2.36E-05

IGF1 0 0.000581 NA NA 0.000045 0.030186 0.000653

IGF1R 0.125869 0.61132 NA NA 0.200267 0.063373 0.004743

IGF2 0.137738 0.196146 NA NA 0.034197 0.0625 0.11744

KDR 0.000375 0.000233 0.000274 0.000304 0.01038 0.000686 0.001532

MET 4.46915 0.920188 NA NA 0.450625 0.019915 0.057115

MMP1 0.021642 0.00162 NA NA 0.45376 0.00093 0.002981

MMP2 0.162668 0.038741 0.67301 0.009119 0.000419 0.001381 0.000509

NCAM1 0.000104 9.58E-05 NA NA 9.7E-06 0.039555 0.010027

PDGFRa 8.51E-05 0.001011 4.55E-06 0.001835 0.004016 0.018581 0.001253

PDGFRb 0.000862 0.002559 NA NA 0.019915 0.003521 0.001025

PLAU 1.34723 1.40444 NA NA 2.32947 0.007139 0.004581

PLAUR 0.316439 0.632878 NA NA 0.757858 0.080772 0.008201

SERPINE1 0.096723 7.51618 NA NA 2.82843 0.008432 0.001069

SOX9 0.858565 0.000145 NA NA 0.429283 0.149685 0.004395

SPRY1 0.234881 0.00982 NA NA 0.061214 0.039282 0.014989

SPRY2 0.271684 0.035403 NA NA 0.297302 0.017098 0.029157

SPRY3 0.008432 0.012604 0.001365 0.045286 0.004518 0.006087 0.015843

SPRY4 0.020334 0.002981 NA NA 0.018581 0.001861 0.000821

TGFa 0.118257 0.120742 NA NA 0.034915 0.027776 0.087172

TNC 0.01541 0.737135 NA NA 0.146604 0.020617 0.00613

VIM 69.551 5.54044 0.091157 0.065954 44.3235 2.39496 0.463294 MSTO- NCI- NCI- NCI- NCI- NCI- NCI-

Gene 211H H1581 HI 703 H2126 H226 H358 H441

AREG 0.0017 0.000868 1.87E-05 0.064704 0.013048 5.73582 2.44528

CA12 0.084788 0.084202 0.000012 0.003645 0.015734 0 0

CDH1 0.009618 0.073302 0.000772 1.81504 0.042986 12.7286 9.84916

DKK3 4.11246 0.127626 0.094732 0.000255 0.161544 0 3.71E-05

DUSP4 0.000309 0.000219 0.0007 0.045123 0.003496 0.040386 0.013508

DUSP5 0.186856 0.02797 0.02977 0.02836 0.174343 0.223756 0.190782

DUSP6 0.255253 1.47427 0.149685 0.062935 0.063813 4.34694 2.86791

EGF 0.003595 0.000997 0.00011 0.000542 0.00982 0.07966 0.049721

EGFR 1.56917 0.108819 0.34151 0.460094 3.05252 0.628507 0.895025

ELK3 0.473029 0.214641 0.376312 0.063813 0.435275 0.463294 0.329877

ELK4 3.97E-05 3.55E-05 0.000788 2.85E-05 4.44E-05 2.64E-05 0.00181

ERBB2 0.189465 0.368567 0.246558 0.20733 0.156041 0.641713 0.482968

ERBB3 0.011125 0.208772 0.00942 0.099442 0.073812 0.721965 0.447513

EREG 0 0.000157 1.41E-05 2.93E-05 0.000145 0.907519 1.18099

ETV4 0.063813 0.408951 0.466516 0.019641 0.166086 0.230047 0.148651

ETV5 0.15932 0.271684 0.907519 0.03125 0.293209 0.183011 0.20733

FGF1 0.007813 0.00564 0.002668 0.000158 0.016289 0.0819 0.006849

FGF10 0.000194 0.000546 9.58E-05 1.35E-05 0.000343 7.31E-05 3.58E-05

FGF11 0.022876 0.301452 0.001543 0.00282 0.005486 0.042394 0.019641

FGF16 0.002079 0.000523 7.46E-05 0.000239 0.002372 0.00029 0.001099

FGF17 4.32E-05 0.00879 0.001887 0.000117 0.001091 0.002307 1.46E-05

FGF18 0.005373 0.119908 0.005154 0.000549 0.619854 0.000686 0.000816

FGF19 9.25E-05 0.01038 2.25E-05 5.7E-06 3.63E-05 0.000804 0

FGF2 3.07375 0.528509 0.069348 7.26E-05 2.12874 0.000273 4.63E-05

FGF20 0.008432 0.121582 0.000174 0 0 0.000478 3.36E-05

FGF21 0 2.01E-05 6.28E-05 0.000003 7.94E-05 0 9.9E-06

FGF22 0.004158 0.009685 0.003173 0.00162 0.014082 0.00292 0.004843

FGF3 0 0.000109 0 1.12E-05 6.28E-05 3.48E-05 0

FGF4 0 0 0 0 0 0 0

FGF5 0.939523 0.00181 0.514057 0 0.148651 0.002595 0

FGF6 0 1.11E-05 2.68E-05 4.1E-06 3.03E-05 0 0

FGF7 0.013322 0.001609 0.000378 2.87E-05 4.11E-05 0.000112 2.08E-05

FGF8 0.000472 0.10083 0.000495 1.39E-05 0.000193 0.000397 0.000695

FGF9 0.002008 0.001253 0.000146 0.000234 0.028164 0.070316 0.010599

FGFBP1 0.111105 0.000782 2.77E-05 0.007239 0.469761 2.88786 0.607097

FGFBP2 0.000109 0.000478 4.03E-05 0.002743 0.000318 0.00128 0.000296 MSTO- NCI- NCI- NCI- NCI- NCI- NCI-

Gene 211H H1581 HI 703 H2126 H226 H358 H441

FGFBP3 0.004187 3.20428 0.001797 0.000597 0.00012 0.002405 0.001773

FGFR1 3.75809 2.05623 1.76541 0.146604 3.70635 0.607097 0.397768

FGFRlIIIb 0.000593 5.54E-05 0.000228 0.000589 0.00141 0.000362 0.001654

FGFRlIIIc 1.33793 1.17283 0.521233 0.011842 1.12506 0.045437 0.048027

FGFR2 0.002152 4.85678 0.02936 0.00071 0.023196 0.033726 0.001861

FGFR2IIIb 0.000644 0.303549 0.001773 0.000277 0.009355 0.020054 0.001106

FGFR2IIIC 0.000345 3.78423 0.008974 4.35E-05 0.006302 0.000531 0.000173

FGFR3 0.008315 0.043586 0.277392 0.051119 0.086569 0.156041 0.00367

FGFR3IIIb 7.57E-05 0.001835 0.01278 0.00849 0.005719 0.009889 2.23E-05

FGFR3IIIC 0.00088 0.006615 0.035403 0.000026 0.003377 0.000443 0

FGFR4 0.001343 0.004645 0.010309 0.005048 0.001642 0.004581 0.004334

FLRT1 0.004044 0.029564 0.036906 0.027017 0.002743 0.016863 0.033961

FLRT2 0.028164 0.008729 0.41466 0.013048 0.11908 0.118257 0.077482

FLRT3 2.77E-05 0.002559 0.001114 0.190782 0.001665 0.005226 0.005563

HGF 6.59E-05 0.005524 2.44E-05 0.00013 0 0 0

IGF1 0 0.006801 9.71E-05 0.000005 3.97E-05 0.030186 0.008729

IGF1R 0.275476 0.965936 0.021793 0.179244 0.840896 0.737135 0.211686

IGF2 2.36199 0.047366 0.005448 0.048361 0.023357 0.214641 3.94E-05

KDR 0.001253 0.004044 4.03E-05 8.63E-05 0.036398 0.5 0.271684

MET 1.75321 0.017337 0.128514 0.173139 2.53151 0.558644 4.82323

MMP1 0.035403 0.022718 0.307786 0.002542 0.058315 0.503478 0.001797

MMP2 3.11666 0.004809 0.003906 0.001099 0.078563 0 0

NCAM1 0.002524 0.000174 5.13E-05 0.000413 0.000856 0.000264 0.000169

PDGFRa 0.005962 0.486327 6.45313 0.000142 0.001926 0.001253 6.73E-05

PDGFRb 0.392292 0.178006 0.000627 0 0.267943 0.004518 0.001654

PLAU 1.6358 0.641713 0.00471 0.054788 0.021793 1.43396 3.53081

PLAUR 0.646176 0.11908 0.143587 0.447513 2.23457 0.773782 0.732043

SERPINE1 37.7918 0.275476 1.07923 0.06983 18.1261 0.316439 0.554785

SOX9 0.417544 0.450625 0.006087 0.214641 0.124137 1.45397 0.103665

SPRY1 0.012344 0.50698 0.185565 0.010525 0.00879 0.119908 0.0625

SPRY2 0.044502 0.030186 0.021642 0.062068 0.019641 0.186856 0.161544

SPRY3 0.001522 0.007289 0.01278 0.004016 0.003472 0.00296 0.001797

SPRY4 0.002323 0.009291 0.015093 0.000288 0.001091 0.004843 0.00471

TGFa 0.001161 0.008088 0.000581 0.01937 0.010097 0.320857 0.521233

TNC 0.02352 0.003262 3.76E-05 0.007546 0.100134 2.14355 1.07923

VIM 78.249 21.8566 32.6724 0.110338 19.8353 5.38893 0.479632 NCI- NCI- NCI-

Gene H460 H520 H522 U-118 U-251 U-87 Y79

AREG 0.052556 0.05329 0.111878 0.000605 0.000065 4.3E-06 9.9E-06

CA12 0.082469 0.003906 0.010237 0.659754 0.087172 1.02811 0.358489

CDH1 0.004809 0.111105 0.005839 6.02E-05 0.007867 0.000181 0.000121

DKK3 0.017824 0.091505 0.017098 5.20537 1.51572 0.089003 0.00026

DUSP4 0.059129 0.002668 3.73E-05 0.000343 0.005448 0.01468 0.001785

DUSP5 0.032129 0.013697 0.016863 0.021642 0.094732 0.06164 0.046391

DUSP6 0.30566 1.49485 0.946058 0.273573 0.63728 0.476319 0.001491

EGF 0.07966 0.01176 5.35E-05 0.014885 0.15822 0.005083 2.14E-05

EGFR 0.11344 0.017948 0.473029 0.673617 0.993092 0.48971 0

ELK3 0.055169 0.006302 0.096055 0.368567 0.25349 0.084788 0.008669

ELK4 7.62E-05 7.41E-05 0 1.49E-05 4.89E-05 0.000129 4.1E-06

ERBB2 0.04181 0.049378 0.348686 0.169575 0.111878 0.005013 0.005962

ERBB3 0.001773 0.018841 0.011518 0.002275 0.019641 0.000416 0.001913

EREG 0.01698 3.03E-05 0.089622 0.034435 0.004216 0.395021 1.44E-05

ETV4 0.5 0.312083 0.266093 0.003424 0.085971 0.026278 0.00015

ETV5 0.133972 0.858565 0.056328 0.156041 1.09429 0.271684 0.003065

FGF1 0.000192 0.002137 0.001511 0.035649 0.120742 NA 0.000388

FGF10 3.55E-05 0.000233 0.02683 0.000236 0.000482 NA 0

FGF11 0.007289 0.010672 0.072796 0.00176 0.025033 0.003401 0.005759

FGF16 0.001554 0.00176 0.000383 0.000163 0.000225 NA 0.000112

FGF17 0.000176 0.006615 0.000288 4.14E-05 0.002421 NA 0.000681

FGF18 0.001665 0.055939 0.002065 0.039282 0.014378 NA 0.004487

FGF19 8.22E-05 0.447513 2.79E-05 0 0.000167 NA 0.000231

FGF2 0.162668 0.125 1.02101 0.325336 0.456916 NA 0.021493

FGF20 0 0.070805 0.000892 0.000104 0.001362 NA 1.27E-05

FGF21 5.28E-05 0.002022 0 6.28E-05 0.00012 NA 0.00002

FGF22 0.001913 0.028164 0.005719 0.001848 0.006708 NA 0.018073

FGF3 0 3.29436 9.6E-06 0 0 NA 7.2E-06

FGF4 0 0.000147 0 0 0 NA 0

FGF5 0 0.00052 0.000042 0.230047 0.032577 NA 5.7E-06

FGF6 4.5E-06 4.32E-05 0 4.4E-06 1.81E-05 NA 6.9E-06

FGF7 0.00143 0.001106 3.07E-05 0.00294 0.001554 NA 7.5E-06

FGF8 0.000148 0.002197 0.001236 8.94E-05 0 NA 0.006172

FGF9 0.001106 0.217638 0.03983 0.000886 0.001289 NA 0.000341

FGFBP1 0.000943 0.02352 6.32E-05 0.000113 0.000475 7.78E-05 0.000019

FGFBP2 0.002307 0.000943 0.000977 0.001271 0.002079 0.000502 0.000411 NCI- NCI- NCI-

Gene H460 H520 H522 U-118 U-251 U-87 Y79

FGFBP3 0.017824 0.008549 0.00982 0.00639 0.007239 0.005263 0.00128

FGFR1 0.101531 7.46426 4.16986 1.25701 1.81504 NA 0.10083

FGFRlIIIb 1.47E-05 0.11744 0.000217 0.000104 0.000527 NA 1.02E-05

FGFRlIIIc 0.020054 2.17347 3.83706 0.952638 0.231647 NA 0.032804

FGFR2 0.001631 0.006003 0.004129 0.00088 0.082469 NA 0.044502

FGFR2IIIb 7.57E-05 0.001848 0.000462 0.000103 0.007189 NA 0.004876

FGFR2IIIC 0.000402 0.000109 0.003853 0.000488 0.023036 NA 0.016516

FGFR3 0.004016 0.291183 0.01937 0.002291 0.373712 NA 0.029977

FGFR3IIIb 5.35E-05 0.049378 0.00044 1.12E-05 0.010672 NA 0.000756

FGFR3IIIC 0.000136 0.000299 0.002981 1.55E-05 0.021051 NA 0.000899

FGFR4 0.000715 0.007041 0.001047 7.16E-05 0.001748 NA 0.006003

FLRT1 0.0625 0.020475 0.012517 0.001848 0.016747 0.006434 0.015625

FLRT2 0.395021 0.001381 0.006944 0.329877 0.033262 0.084788 0.007239

FLRT3 0.000618 0.00074 0.00072 0.000108 0 0.002108 0.000223

HGF 2.87E-05 0.007391 0.011679 1.19748 0.000411 1.32869 2.01E-05

IGF1 0 5.02805 0.001689 0.070805 0.015303 0.00471 0.000226

IGF1R 0.368567 0.028956 1.34723 0.041521 0.668964 0.052193 0.142595

IGF2 0.008258 0.00357 0.05872 0.097396 0.000459 0.000197 0.035649

KDR 0.007705 0.001145 0.000196 0.001228 0.003308 0.000108 0.00004

MET 0.262429 0.066064 0.089622 1.3566 0.366021 0.697372 0.00088

MMP1 0.00639 0.000125 0.033493 0.104386 0.003906 0.049378 5.4E-06

MMP2 0.006708 0.139661 0.003545 5.61778 2.37841 10.9283 0.001289

NCAM1 0.022251 0.02836 8.7E-06 0.000446 0.125 0.004016 0.030186

PDGFRa 0 0.001325 0.005759 1.07177 0.650671 0.120742 0.000121

PDGFRb 0.021945 0.00148 0.002152 3.50642 0 1.28343 0.000338

PLAU 0.011598 0.000226 0.021493 1.46409 0.933033 2.56685 8.57E-05

PLAUR 0.098755 0.022718 0.003826 0.190782 0.939523 0.933033 0.041235

SERPINE1 0.044811 0.010027 0.003002 1.54756 3.53081 2.2974 2.46E-05

SOX9 0.535887 0.496546 0.02797 0.119908 3.34035 0.30566 0.000983

SPRY1 0.010097 0.001532 0.334482 0.070316 0.092783 0.003496 0.019505

SPRY2 0.028956 0.115024 0.008851 0.092783 0.432269 0.351111 0.017458

SPRY3 0.046391 0.015517 0.001785 0.001598 0.009291 0.007813 0.004425

SPRY4 0.00181 0.007239 0.002668 0.002065 0.002879 0.002197 0.00012

TGFa 0.001665 0.099442 0.021793 0.002259 0.266093 0.024689 0.000296

TNC 0 0.000531 0.001609 2.62079 2.32947 4.02782 0.000341

VIM 13.0864 2.71321 5.1337 31.3414 48.1679 22.4711 0.790041

Claims

1. A method of treating cancer having an FGFRl gene amplification in a subject, wherein an FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule, comprising:

administering a therapeutically effective amount of an FGFRl ECD or an FGFRl ECD fusion molecule to the subject.

2. A method of treating cancer in a subject, comprising:

administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFRl) extracellular domain (ECD) or an FGFRl ECD fusion molecule to the subject, wherein, prior to administration of the FGFRl ECD or FGFRl ECD fusion molecule, at least a portion of the cells of the cancer have been determined to have an FGFRl gene amplification, and

wherein an FGFRl gene amplification in a cancer is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

3. The method of claim 1 or claim 2, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least three copies of the FGFRl gene.

4. The method of claim 3, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least four copies of the FGFRl gene.

5. The method of claim 3, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least five copies of the FGFRl gene.

6. The method of claim 3, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least six copies of the FGFRl gene.

7. The method of claim 3, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least eight copies of the FGFRl gene.

8. The method of claim 1 or claim 2, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification have a ratio oiFGFRl gene to chromosome 8 centromere of at least 1.5.

9. The method of claim 8, wherein the ratio oiFGFRl gene to chromosome 8 centromere is at least 2.

10. The method of claim 8, wherein the ratio oiFGFRl gene to chromosome 8 centromere is at least 2.5.

1 1. The method of claim 8, wherein the ratio oiFGFRl gene to chromosome 8 centromere is at least 3.

12. The method of claim 8, wherein the ratio oiFGFRl gene to chromosome 8 centromere is at least 3.5.

13. The method of claim 8, wherein the ratio oiFGFRl gene to chromosome 8 centromere is at least 4.

14. The method of claim 2, wherein FGFR1 gene amplification was determined by a method selected from fluorescence in situ hybridization, array comparative genomic hybridization, DNA microarray, spectral karyotyping, quantitative PCR, southern blotting, or sequencing.

15. The method of any one of the preceding claims, wherein the cancer overexpresses at least one, at least two, at least three, at least four, or at least five markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4.

16. The method of any one of the preceding claims, wherein the cancer overexpresses at least one, at least two, at least three, or at least four markers selected from FGFR1, FGFR3IIIC, FGF2, DKK3, and FGF18.

17. The method of any one of claims 1 to 15, wherein the cancer overexpresses

ETV4.

18. The method of claim 15, wherein the cancer overexpresses at least two, at least three, at least four, or at least five markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4.

19. The method of any one of claims 15, 16, and 18, wherein FGFR1 is

FGFRlIIIc.

20. A method of treating cancer that overexpresses at least one, at least two, at least three, at least four, or at least five markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in a subject, wherein overexpression of at least one marker selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) or an FGFR1 ECD fusion molecule, comprising:

administering a therapeutically effective amount of an FGFR1 ECD or an FGFR1 ECD fusion molecule to the subject.

21. A method of treating cancer in a subject, comprising:

administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) or an FGFR1 ECD fusion molecule to the subject, wherein, prior to administration of the FGFR1 ECD or FGFR1 ECD fusion molecule, at least a portion of the cells of the cancer have been determined to overexpress at least one, at least two, at least three, at least four, or at least five marker selected from FGFR1, FGFR3IIIC, FGF2, DKK3, FGF18, and ETV4, and

wherein overexpression of at least one marker selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in a cancer is indicative of therapeutic responsiveness by the cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule.

22. The method of claim 20 or claim 21, wherein the cancer overexpresses at least two, at least three, at least four, or at least five markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, FGF 18, and ETV4.

23. The methof of any one of claims 20 to 22, wherein the cancer overexpresses

ETV4.

24. The method of claim 20 or claim 21, wherein the cancer overexpresses at least one, at least two, at least three, or at least four markers selected from FGFR1, FGFR3IIIc, FGF2, DKK3, and FGF18.

25. A method of treating cancer that overexpresses FGF2, wherein FGF2 overexpression is indicative of therapeutic responsiveness by the cancer to a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) or an FGFR1 ECD fusion molecule, comprising:

26. A method of treating cancer in a subject, comprising:

administering a therapeutically effective amount of a fibroblast growth factor receptor 1 (FGFR1) extracellular domain (ECD) or an FGFR1 ECD fusion molecule to the subject, wherein, prior to administration of the FGFR1 ECD or FGFR1 ECD fusion molecule, at least a portion of the cells of the cancer have been determined to overexpress FGF2, and wherein FGF2 overexpression is indicative of therapeutic responsiveness by the cancer to an FGFR1 ECD or FGFR1 ECD fusion molecule.

27. The method of claim 25 or claim 26, wherein the cancer does not have an FGFR1 gene amplification.

28. The method of any one of claims 20 to 27, wherein the overexpression is protein overexpression.

29. The method of claim 28, wherein protein overexpression is determined using immunohistochemistry.

30. The method of any one of claims 20 to 27, wherein the overexpression is mRNA overexpression.

31. The method of claim 30, wherein mRNA overexpression is determined using quantitative RT-PCR.

32. The method of any one of claims 20 to 31 , wherein the cancer has an FGFRl gene amplification.

33. The method of claim 32, wherein at least a portion of the cells of the cancer having an FGFRl gene amplification comprise at least three, at least four, at least five, at least six, at least seven, or at least eight copies of the FGFRl gene.

34. The method of any one of the preceding claims, wherein the method further comprises administering at least one additional therapeutic agent.

35. The method of claim 34, wherein at least one additional therapeutic agent is selected from docetaxel, paclitaxel, vincristine, carboplatin, cisplatin, oxaliplatin, doxorubicin, 5-fluorouracil (5-FU), leucovorin, pemetrexed, etoposide, topotecan, sorafenib, a VEGF antagonist, a VEGF trap, an anti-VEGF antibody, and bevacizumab.

36. The method of any one of the preceding claims, wherein the method comprises administering an FGFRl ECD.

37. The method of claim 36, wherein the FGFRl ECD comprises an amino acid sequence selected from SEQ ID NOs: 1 to 4.

38. The method of any one of claims 1 to 35, wherein the method comprises administering an FGFRl ECD fusion molecule.

39. The method of claim 38, wherein the FGFRl ECD fusion molecule comprises an FGFRl ECD and a fusion partner, and wherein the fusion partner is Fc.

40. The method of claim 39, wherein the FGFRl ECD fusion molecule comprises a sequence selected from SEQ ID NO: 5 and SEQ ID NO: 6.

41. The method of any one of the preceding claims, wherein the cancer is selected from lung cancer, renal cancer, colon cancer, liver cancer, breast cancer, ovarian cancer, endometrial cancer, esophageal cancer, head and neck cancer, glioblastoma, and prostate cancer.

42. The method of claim 41, wherein the cancer is lung cancer, breast cancer, head and neck cancer, renal cancer, or esophageal cancer.

43. The method of claim 42, wherein the cancer is lung cancer.

44. The method of claim 43, wherein the lung cancer is non-small cell lung cancer.

45. The method of claim 44, wherein the lung cancer is small cell lung cancer.

46. A method of identifying a subject with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule, comprising determining the number of copies of an FGFRl gene in at least a portion of the cancer cells in a sample obtained from the subject, wherein greater than 2 copies of the FGFRl gene in a cell indicates that the cell has FGFRl gene amplification, and wherein FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

47. A method of identifying a subject with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule, comprising determining the ratio of FGFRl gene to chromosome 8 centromere in at least a portion of the cancer cells in a sample obtained from the subject, a ratio of greater than 1 in a cell indicates that the cell has FGFRl gene amplification, and wherein FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

48. The method of claim 46 or claim 47, wherein the number of copies of an FGFRl gene or the ratio of FGFRl gene to chromosome 8 centromere is determined by a method selected from fluorescence in situ hybridization, array comparative genomic hybridization, DNA microarray, spectral karyotyping, quantitative PCR, southern blotting, or sequencing.

49. The method of any one of claims 46 to 48, further comprising determining the level of at least one, at least two, at least three, at least four, or at least five proteins or mRNAs selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in at least a portion of the cancer cells in a sample obtained from the subject, wherein overexpression of at least one protein or mRNA selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

50. The method of claim 49, wherein the method further comprises determining the level of at least one, at least two, at least three, or at least four proteins or mRNAs selected from FGFRl, FGFR3IIIc, FGF2, DKK3, and FGF18.

51. The method of claim 49, wherein the method further comprises determining the level of ETV4.

52. A method of identifying a subject with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule, comprising determining the level of at least one, at least two, at least three, at least four, or at least five proteins or mRNAs selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 in at least a portion of the cancer cells in a sample obtained from the subject, wherein overexpression of at least one protein or mRNA selected from FGFRl, FGFR3IIIc, FGF2, DKK3, FGF18, and ETV4 is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

53. The method of claim 52, wherein the method comprises determining the level of at least one, at least two, at least three, or at least four proteins or mR As selected from FGFRl, FGFR3IIIC, FGF2, DKK3, and FGF18.

54. The method of claim 52, wherein the method comprises determining the level of ETV4.

55. The method of claim 52 or claim 53, wherein FGFRl is FGFRlIIIc.

56. A method of identifying a subject with cancer who may benefit from administration of an FGFRl ECD or FGFRl ECD fusion molecule, comprising determining the level of FGF2 in at least a portion of the cancer cells in a sample obtained from the subject, wherein overexpression of FGF2 is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

57. The method of claim 56, further comprising determining that the cancer does not have an FGFRl gene amplification.

58. The method of any one of claims 49 to 57, wherein the level of one or more proteins is determined.

59. The method of claim 58, wherein the level of protein is determined using immunohistochemistry.

60. The method of any one of claims 49 to 57, wherein the level of one or more mRNAs is determined.

61. The method of claim 60, wherein the level of mRNA is determined using quantitative RT-PCR.

62. The method of any one of claims 49 to 56, further comprising determining the number of copies of an FGFRl gene in at least a portion of the cancer cells in a sample obtained from the subject, wherein greater than 2 copies of the FGFRl gene in a cell indicates that the cell has FGFRl gene amplification, and wherein FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

63. The method of any one of claims 49 to 56, further comprising determining the ratio of FGFRl gene to chromosome 8 centromere in at least a portion of the cancer cells in a sample obtained from the subject, a ratio of greater than 1 in a cell indicates that the cell has FGFRl gene amplification, and wherein FGFRl gene amplification is indicative of therapeutic responsiveness by the cancer to an FGFRl ECD or FGFRl ECD fusion molecule.

64. The method of any one of claims 46 to 63, wherein the FGFRl ECD comprises an amino acid sequence selected from SEQ ID NOs: 1 to 4.

65. The method of any one of claims 46 to 63, wherein the FGFRl ECD fusion molecule comprises an FGFRl ECD and a fusion partner, and wherein the fusion partner is Fc.

66. The method of claim 65, wherein the FGFRl ECD fusion molecule comprises a sequence selected from SEQ ID NO: 5 and SEQ ID NO: 6.

67. The method of any one of claims 46 to 66, wherein the cancer is selected from lung cancer, renal cancer, colon cancer, liver cancer, breast cancer, ovarian cancer, endometrial cancer, esophageal cancer, head and neck cancer, glioblastoma, and prostate cancer.

68. The method of claim 67, wherein the cancer is lung cancer, breast cancer, renal cancer, head and neck cancer, or esophageal cancer.

69. The method of claim 68, wherein the cancer is lung cancer.

70. The method of claim 69, wherein the lung cancer is non-small cell lung cancer.

71. The method of claim 69, wherein the lung cancer is small cell lung cancer.