WO2013082035A1

WO2013082035A1 - Method and composition for increasing the proportion of dietary ingredients that are resistant to degradation by ruminal microorganisms

Info

Publication number: WO2013082035A1
Application number: PCT/US2012/066661
Authority: WO
Inventors: James S. Drouillard; Dan A. KLAMFOTH; Kevin D. Ingram
Original assignee: LHOIST NORTH AMERICA Inc; Kansas State University
Current assignee: LHOIST NORTH AMERICA Inc; Kansas State University
Priority date: 2011-11-28
Filing date: 2012-11-27
Publication date: 2013-06-06
Anticipated expiration: 2014-05-28
Also published as: EP2787835A1; AU2012346157A1; AU2012346157B2; CL2014001385A1; CA2855883C; RU2626950C2; MY173226A; RU2014125809A; BR112014012733A8; CN104219960A; ZA201404537B; EP2787835A4; CO7101224A2; BR112014012733A2; NZ626354A; US20130136827A1; CA2855883A1; US20140335230A1

Abstract

Feed ingredients that are otherwise susceptible to degradation by ruminai microorganisms are combined with mineral hydrates (or oxides) and water, and processed through a pin mixer, pellet mill, extruder, or other suitable device to produce agglomerated particles. The ruminant animal feed which is so produced effectively increases the proportion of dietary ingredients presented for digestion and absorption within the post-ruminal digestive tract of the animal by inhibiting premature digestion by microorganisms inhabiting the rumen.

Description

METHOD AND COMPOSITION FOR INCREASING THE PROPORTION OF DIETARY INGREDIENTS THAT ARE RESISTATNT TO DEGRADATION BY RUMINAL

M!CROORGA ISMS

Technical Field The present invention relates generally to ruminant feedstocks for domesticated ruminants and, particularly, to such feedstocks which are resistant to degradation by ruminal microorganisms.

Background Art

Ruminant animals, including cattle, sheep, goats, deer, and buffalo, have a highly specialized and complex stomach, portions of which are inhabited by microorganisms capable of digesting complex carbohydrates, such as ceiiulose (fiber). The stomach of ruminants is divided into four distinct chambers— the rumen, reticulum, omasum, and abomasum. The first two of these compartments are characterized by the presence of dense populations of symbiotic bacteria, archaea, protozoa, and fungi. These microorganisms are capable of fermenting feeds that are ingested by ruminant animals, ultimately yielding metabolites that can be used by other microorganisms or the host animal, it is this symbiotic relationship that renders ruminants capable of producing milk, meat, and other products while eating fibrous feeds that cannot be digested by pigs, chickens, people, and other simple-stomached, monogasiric animais.

One of the challenges in production of ruminant animals is in balancing nutritional requirements of microorganisms in the gut with those of the host animal. High producing ruminants require substantial quantities of amino acids, energy, vitamins, and minerals to meet demands for production of milk, meat, and (or) fiber, The microbes within the rumen (i.e., reticulo-rumen) are very adept in their ability to degrade carbohydrates, protein, and other constituents of the diet, often to an extent thai far exceeds their own nutrient needs. Excessive degradation of nutrients by ruminal microorganisms can result in relative deficiencies of these nutrients for the ruminant host. Protein, amino acids, and certain vitamins are particularly susceptible to microbial degradation within the rumen. As an example, dietary proteins are extensively degraded by .microorganisms to yield amino acids, which then are deaminated to yield ammonia. The ammonia is utilized by microflora and fauna of the rumen ecosystem for synthesis of microbial protein, but when produced in excess is absorbed into the bloodstream, converted to urea by the liver, and excreted in urine via the kidneys as a waste product. If excessive degradation is avoided, these amino acids exit the rumen and become available for absorption within the small intestine, thereby contributing to the nutrient requirements of the host animal Various means have been employed to modify dietary ingredients in ways that decrease their susceptibility to microbial degradation within the rumen, thus increasing the proportion of the compound or ingredient that "bypasses" the rumen. Ruminally undegraded, rumen undegradab!e, ruminally protected, escape, and bypass all are terms used to describe compounds or products that exhibit some degree of resistance to the digestive actions of microorganisms withi the rumen,

In spite of these advances in the relevant arts, a need continues to exist for further improvements in techniques for increasing th proportion of dietary ingredients that are resistant to degradation by ruminai microorganisms.

Disclosure of the invention

In the method of the present invention, feed ingredients that are otherwise susceptible to degradation by ruminai microorganisms are combined with calcific and/or doiomitic mineral hydrates generieaily called hydrated lime as a binder, and typically with a blending aid, such as water. The mixture is then processed through a pin mixer, pellet mills disc pelletizer, drum pelletizer, extruder, or other suitable device to produce prills or pellets of agglomerated particles. The hydrated lime which is used in the method of the invention can be a high calcium, doiomitic or partially hydrated doiomitic lime produced in a pressure hydrator or in an atmospheric hydrator. This would include hydrates made from magnesium lime and/or calcific doiomitic lime, i.e,, high calcium lime, magnesium lime, caicitie doiomitic lime and doiomitic lime. While some mixtures of component ingredients used in the practice of the invention will contain the previous components alone, some mixtures will also include a caicitie and/or doiomitic carbonate mineral component, i.e., dolomite, calcium carbonate or magnesium carbonate or mixtures thereof. This method of processing ruminant animal feed and the feed product produced thereby effectively increases the proportion of dietary ingredients present in the feed that are resistant to degradation by ruminal microorganisms.

There are a number of additional processing steps which may be employed, depending upon the desired characteristics of the end product. For example, the agglomerated particles may have a secondary coating applied after agglomeration. The processing technique can be used to protect other ingredients from the action of ruminal microorganisms. For example, the agglomerated particles may also include lysine, methionine or other amino acids as a means of increasing the proportion of those compounds that are available for absorption in the animal postruminal tract. The agglomerated particles may include choline and water soluble vitamins that may be required by the animal in quantities that exceed those which would normally escape digestion by ruminal microbes. The agglomerated particles so produced may also provide for the protection of monounsaturate or polyunsaturated lipids which normally are extensively biohydrogenated by ruminal microorganisms to yield saturated lipids. The same techniques can be used to provide for the protection of fat soluble vitamins, enzymes, probiotics, prebiotics, carbohydrates, pharmaceuticals, essential oils, minerals, and other compounds which insure that a greater proportion of these products are presented post-ruminally.

Additional objects, features and advantages will be apparent in the written description which follows.

Brief Description of the Drawings

Figure 1 is a graphical representation of the results of an In situ evaluation of the disappearance of dry matter after 24 hours of incubation in the rumen.

Figure 2 is a graph of fatty acid concentration in plasma of growing steers. Description of the Preferred Embodiment

The embodiments herein and the various features and advantageous details thereof are explained more fully with reference to the non-limiting embodiments that are detailed in the following description. Descriptions of well-known components and processes and manufacturing techniques are omitted so as to not unnecessarily obscure the embodiments herein. The examples used herein are intended merely to facilitate an understanding of ways in which the invention herei may be practiced and to further enable those of skill in the art to practice the embodiments herein. Accordingly, the examples should not be construed as limiting the scope of the claimed invention.

In the present invention, "animal feed ingredients" that are otherwise susceptible to degradation by ruminal microorganisms are combined with calcific and/or doiomitic mineral hydrates genericaily called hydrated lime as a binder, and typically with a blending aid, such as water. The mixture is then processed through a pin mixer, pellet mill, disc pelletizer, drum pelletizer, extruder, or othe suitable device to produce prills or pellets of agglomerated particles. In the case of a pin mixer, a mixture of dry powders will usually be charged to the mixer with water being injected via injection ports on the top of the pin mixer. However, either method of pre-mixing the water or adding the water during processing can be employed. Solubi!izable products can be pre-solubiiized and then injected with the water via the injection ports (for example, lysine has been successfully processed in this manner, as well as in the standard dry mix manner with water being injected via the injection ports). Semi-dry (pre-wetted) products can also be used in a disc pelletizer or a drum pelletizer. In some cases, water is not required, as where high moisture ingredients are combined with the other dry ingredients. Non-aqueous solvents, such as glycerol, may also be employed in some circumstances.

By "animal feed ingredient" is meant in this discussion that component of the agglomerated prill or pellet that would otherwise be susceptible to degradation by ruminal microorganisms/enzymes in the rumen, These ingredients will include such things as biologically active ingredients and/'or therapeutic or nutritional agents, as well as those ingredients merely having food value, in addition to those "food ingredients" previously mentioned, such ingredients may include mineral additives such as sodium, potassium, iron, calcium; vitamins such as vitamins A,B,D, etc.; protein/energy producing foods such as milted flax seed, dried blood or meat meai, cottonseed meai, soy meal, canoia meai, glucose, fatty acids and yeasts; growth factors; enzymes such as proteases, lipases, or carbohydrases, including but not limited to, amylases, lactases, hemicellulases, xyanases, and cellulases; antibiotics; exogenous growth promotans; and food adjuvants such as sodium bicarbonate, sorbitol, propylene glycol and sodium propionate. The "animal feed ingredient" ca be thought of as^' a core material which is embedded or tied up within a matrix consisting of the carbonate/hydrate complex, in other words, a matrix of agglomerated particles.

The hydrated lime which is used in the method of the invention can be a high calcium, dolomitic or partially hydrated dolomitic lime produced in a pressure hydrator or in an atmospheric hydrator. This would include hydrates made from magnesium lime and calcific dolomitic lime, i.e., high calcium lime, magnesium lime, calcific dolomitic lime and dolomitic lime.

Preferred calcific and dolomite mineral hydrates used as binder component for the food ingredients in making the agglomerated particles of the invention thus include both high calcium hydrate and dolomitic hydrate, as well as mixtures of calcium and magnesium hydroxide. The term "hydrated lime" is therefore intended in this discussion to generally encompass all of the following: High Calcium Hydrate: Hydrated lime (calcium hydroxide, or slaked lime) is a dry powder resulting from the controlled slaking of quicklime with water. The exothermic or released heat of reaction is captured and used to evaporate the excess slaking wafer. This is to be distinguished from "lime slurry" in which the excess water is not evaporated and the hydrate remains as a water suspension. The chemical formula is Ca(OH)₂.

Dolomitic Hydrate: Dolomitic Hydrate is manufactured from doiomitic quickiime basically by two methods. The first method; is similar to high calcium hydrate manufacture and usually does not completely hydrate ail the oxides; especially the magnesium oxide component. The second method relates to pressure hydration of dolomitic quicklime under special bydrating conditions that control temperature and pressure i order to insure that all the calcium and magnesium oxides are fully hydrated. Varieties of hydrates from both methods may be utilized for purposes of the present invention, either those produced by pressure hydrators, or those produced by atmospheric hydrators."

A spectrum of products of the above type are commercially available from Lhoist North America, 3700 Hulen Street, Fort Worth, Texas 76107, or from Lhoist operations worldwide.

As has been mentioned, while some mixtures of component ingredients used in th practice of the invention will contain the previous components alone, some mixtures will also include a caieitic and/or dolomitic carbonate mineral component, i.e., calcium carbonate or magnesium carbonate or dolomite or mixtures thereof. The addition of such a mineral component generally helps in the ultimate pri!l formatio and also yields a stronger prill. Other minerals such as selenium may be included, as well as aluminum containing compounds, in some cases, mineral oxides, e.g., calcium oxide or magnesium oxide, may also be present.

Preferred binder compositions of the inventio will thus typically be comprised of hydrated lime in combination with a companion material or materials, such as, for example, a dolomitic or caieitic limestone. The hydrated lime component will typically be present in the range from 10 to 95% by weight of the total composition, preferably about 25 to 90% weight. By way of an example, the binder composition ca contain about 40% by weight of hydrated lime and 80% by weight dolomitic limestone or dolomite. An example dolomitic limestone is Applicant's "Pro g™ 95" dolomitic limestone which is commercially available from Lhoist North America. Other companion materials include clay(s), magnesium oxide, magnesium carbonate (magnesite) and magnesium hydroxide (brueite). In other circumstances, the binder is made u of the hydrated lime alone with the animal feed ingredient. When combined with the binder component or components of the invention and processed as described, a matrix of agglomerated particles is produced. The end result may be either a pellet or prill as those terms are commonly understood. A "pellet" typically takes the form of a rod or cylinder, while a "prill" wit! be taken to mean a small aggregate of a materia!, most often a dry sphere which is a solid a room temperature. As has been mentioned, it is useful to think of the products of the invention as having a core material (the animal feed ingredient) which is embedded or tied up within a matrix consisting of the carbonate/hydrate complex.

The manufacturing process for manufacturing the agglomerated pellets/prills of the invention will now be described in greater detail. Table ! below gives the settings used for a pin mill in manufacturing the agglomerated particles of the invention. A "pin mill or pi mixer" will be understood by those skilled in the relevant arts to be a high speed, conditioning and micro-pe!letizing device that converts powders into small agglomerates through the action of a high speed rotor shaft and pin assembly, with the addition of liquids such as water, binders, oil or surfactants.

Table !:

Production Run - Settings

50% Dolomitic Hydrate (pressure Mydrated}/50% Milled Flax Seed

Material Feed Rate Noz2le Wsisr Pin Mixer Green Pellets

ti.l 2,0 '.302 is Oil -is 4. SO 2 ISO'S 7:2 >J i4.4^:& 34.3

*4G02 tip -applies 0.2 gallons of water per minute in a 40 degree flat spray pattern (at 40 psi)

*^*Aerated bulk density (lbs/ft³)

Table II below gives the raw material properties for the raw ingredients fed to the pin mixer. Table Π:

Raw Material Properties (Pre-Run

Table II! gives the size distribution information for the ml!ied flax seed which comprises the "animal feed ingredient" which is to be protected from ruminai degradation. Milled flax seed is a commonly available product which can be produced, for example, by processing with a hammer miii. Flax seeds contain high levels of dietary fiber as well as iignans, an abundance of micronutrients and omega~3 fatty acids, Table ill:

Milled Flax Seed Sizing

Tables IV and V below give the finished pellet properties of the pellets produced with the pin mixer: Table I

Finished Pellet Properties

Bulk Density (I /ff') % Attrition Comp. Strength

% Moisture* {Aerated) {Compacted} Loss**- { g&omds

C,S¾ 36.2 45.2 <03% 10,"

^■Cab-dried samples at 90°C

** Measured as % loss of -18X20 mesh after 5 inins on 30 mesh screen (Ro-Tap)

"""Conducted on 1/8"^' pri!ls/peHets (7' samples ~ highest & iowesi dropped) Table V

Finished Pellet Size Distribution

(0.5% moisture - 9G°C lab dried samples)

Sieve Size % Retained % Cumulative (Retained

14 mesh 69.2% 69.2%

16 mesh 9.2% 78.4%

20 mesh 0.3% 88.7%

45 mesh 10.3% 99.0%

80 mesh 0.8% 99.8%

120 mesh 0,2% 99.8%

pan 0.2% 100.0%

The pellets of agglomerated particles so prepared were then used in two test evaluations of the efficacy of the method of the invention in protecting feed ingredients from degradation that would otherwise occur in the animal rumen. The first evaluation was an "in situ" trial. The test pellets were 50% dolomitic lime hydrate/50% milled flax seed; 75% lime hydrate/25% milled flax seed; and 90% lime hydrate/ lysine, respectively. They are compared with flax seeds or lysine alone.

Evaluation No. 1 :

The in situ procedure utilizes small in situ bags made of a nitrogen-free synthetic polyester fabric (Dacron®; Ankom Technology, ivlecedon, NY) that has a 50 m pore size. The pores are sufficiently small such that when feed materials are placed into the bag the contents are retained, The pore size also is large enough to allow for entry of microorganisms into the bag when placed into the rumen, thus exposing the contents to the degradative actions of ruminal microbes. Disappearance of feed particles from the bag is presumed to be due to microbial fermentative activity whilst the bag and its contents are suspended within the rumen environment. In situ assays provide useful information regarding the susceptibility of feeds to microbial digestion within th rurnen<

The test procedure consisted of adding 3.2 g of sample (as is) to Dacron bags, which then were heat sealed and subsequently placed into the rumen and allowed to incubate for 24 hours. Bags then were removed from the rumen, dried and weighed to determine disappearance of dry matter. Concentrations of protein, total fatty acids, and fatty acid profile were determined for the residue from each sample. Samples were prepared in duplicate within each animal, along with blank bags for correction, and six animals were used. Three cattle were fed a high-concentrate diet and 3 were fed a high-forage, i.e., low concentrate diet.

Table VI summarizes dry matter contents, and well as the as-fed and dry matter concentrations of crude protein and total fatty acids for pure ground flaxseed, the 50:50 fiaxseed/Lime mixture, the 75:25 Ftaxseed/Lime mixture; the 90:10 Ume/Lysine mixture, and pure lysine hydrochloride prior to in situ fermentation. These values were used to calculate the extent of dry matter and nutrient disappearance during the in situ digestion procedure. Table VI

Product .} Dry Total Fatty Crude Crude Protein (% Total Fatty Acids

Matter Acids {%) Protein (%} Dry Matter Basis) (^'% Dry Matter I (%) Basis)

Flaxseed | 93,73 43.653 22.375 23.872 46.573

50/50 98,16 10.281 10.165 10.356 10.474

Flaxseed I

75/25 98.23 7.510 5.995 6.103 7.645

Flaxseed |

90/10 I 98,75 0.085 8,884 8.978 0.086

Lysine j

Lysine i 99.38 15.337 15.433 Table VI! summarizes the percent disappearance of dry matter from in situ bags during a 24-hour period of ruminal incubation. Two sets of donor animals were used (High Forage/Low Concentrate and High Concentrate/Low Forage) to evaluate disappearance under varying ruminai conditions. The column identified as "Mean" represents the average of the Low and High concentrate groups. Flaxseed in its unprotected form was between 47.95 and 81.38% ruminally degraded (mean of 54.68%), whereas disappearance of the Lime/F!axseed mixtures ranged from 5.16 to 14.42%, with the greater proportion of lime (i.e., 75%) yielding the greatest ruminal stability Unprotected lysine was almost completely degraded (>99.83%), whereas the !ime/!ysine mixture was substantially more stable within the rumen.

Table VII:

In situ dr matter disa earance % after 24 hours

Table VHI summarizes the fatty acid contents of the unprotected and protected fiax products after 24-hours of in situ incubation. These values were used in conjunction with information from Tables VI and VI to calculate the proportion of fatty acids that were retained through the in situ incubation, which are summarized in Table IX. On average, less than 34% of fatty acids remained after the 24-hour incubation of unprotected flaxseed (range of 27.27-39,95), whereas more than double this amount was retained for the protected fiax products.

Table VIII:

Total fatty acids (%) in residue after 24 hours of incubation

Table IX:

Ruminal escape of fatty acids (%) after 24 hours of incubation

Table X illustrates the concentrations of protein of residue retained in the bags following 24 hours of ruminal incubation. Note that values are zero for the unprotected lysine, indicating that 100% of the material disappeared from the bag. Information in Table X was used in conjunction with data in Tables VI and VII to calculate the fractions of protein that were resistant to ruminal degradation {i.e., ruminal escape protein), which are summarized in Table XI. Lysine in its unprotected form was completely degraded, while the lime treated products were substantially more resistant to degradation. Similarly, protein in the protected forms of flaxseed was approximaiely 2.5-foid more resistant to degradation during the 24-hour in situ incubation period, indicating that the method has substantial efficacy for protecting nutrients against microbial digestion, Table X;

Table XI;

Ruminal escape of crude protein (%) after 2 hours of incubation.

Crude Protein Escape (%)

Product Mean Low Concentrate High Concentrate

Flaxseed 32.184 27.774 36.594

50/50 Flaxseed 81.618 79.517 83.719

75/25 Flaxseed 81 ^"784 82.848 80.720

90/10 lysine 19.456 10.622 28.291

Lysine 0 0 Table XI! summarizes the fatty acid profiles of residues after 24-hour in situ incubation. Notable differences are seen with C18:1n9t, C18:1n11 , and C18:2n6t, ail of which are formed during partial biohydrogenation of aJpha-linolenic acid or linoieic acid by ruminai microbes, in each case, values are lower for the protected forms of flaxseed, indicating that the matrix was an effective microbial barrier. Most notable is the increase in C 8:3n3 (i.e., linolenic acid), which is the predominant polyunsaturated^ fatty acid in flaxseed. Compared to the unprotected form of flaxseed, the lime matrix increased retention of this fatty acid by between 87 and 116%.

Table XII:

Fatty acids appearing in residue following 24 hours of incubation, expressed as percent of the amount initially placed into the rumen. Values are the result of conversion from one fatty acid to another (i.e., biohydrogenation).

ND: not detected 1 The notation used to identify fatty acids is as foi!ows: The number immediately following the letter "Q" Indicates the number of carbon atoms in the fatty acid chain. The number immediately following the colon indicates the number of double bonds between carbon atoms in the fatty acid chain (i.e., degree of saturation}. Omega 3 fatty acids are denoted as "n3°, omega 6 fatty acids as ^! , and so on. The cis and trans configurations of double bonds are denoted as "c" and^: Ύ.

The results of the first evaluation are illustrated graphically in Figure 1 of the drawings. The in situ dry percentage disappearance can be seen to be dramatically lower for the co-priiled milled fiaxseed/!ime hydrate trials or even for lysine/lime hydrate trial, compared to flax seeds or lysine alone, in the next evaluation, a study was conducted to determine If feeding milled flaxseed co-prllied with doiomitic hydrate and doiomiiic carbonate will decrease biohydrogenation of polyunsaturated fatty acids by rumen microorganisms, thus Increasing their concentrations within the rumen blood.

Evaluation No, 2:

Procedure; Forty-five steers were blocked by weight, randomly assigned to individual pens, and pens to dietary treatments (15 replicates), Steers were fed for 14 days with a basal diet consisting of 30.0% wet corn gluten feed, 25% wheat straw, 25% prairie hay, 12.78% steam-flaked corn, and 3.02% supplement, In treatments 2 and 3, a portion of flaked corn was replaced with 2.79% flaxseed or 8.13% of a blend of Lime and Flaxseed according as shown in Table 1. Corn oil was included to provide for similar fat concentrations in the three diets. Diets were formulated to provide at least 12% crude protein, 300 mg/day monensin, 1000 iU/lb vitamin A, 0,1% added sodium, and 0.15% added chlorine, 0.7% calcium, G.7% potassium, and 10 ppm of Cu. Weights of unconsumed feed {oris} were determined every day. Weekly samples of feeds were taken and composited sample per treatment thai was analyzed for dry matter (DM), organic matter (O!VI), crude protein (CP), neutral detergent fiber (NOF), and total lipids. Blood samples were taken from the jugular vein for analysis of long chain fatty acid (LCFA) concentrations on day 0, 7, and 14 of the study. Heparinized vacuum tubes (green top) were used which immediately placed on ice and centrsfuged (1200 x g for 20 min). On day 16 of the study, and 3 h after feeding, samples of ruminal fluid and ruminal headspace gas were taken by rumenocentesis in order to determine ruminal pH, LCFA profile of ruminal digesta, and gas composition.

Data were statistically analyzed using the MIXED procedure of SAS (Version 9.0 with treatment and day as fixed effects, bam nested within strata, barn as the random effect, and animal as the experimental unit.

Table XIII:

Diets.

Flaxseed/

Ingredients, % Pre-experiment Control Flaxseed Lime

Wet corn gluten feed 30.00 30.00 30.00 30.00

Wheat stra 25.00 25.00 25.00 25.00

Prairie hay 25.00 25.00 25.00 25.00

Steam flaked corn 10.36 12.78 12.86 8.50

Linseed meal 3.01 1.22 1.51

Corn oil 1.19 0.1 -

Flaxseed .... .... 2.79 —

Lime/Flax — — — 8.13

Glycerin 5.00 — —

Supplement³ 4.64 3.017 3.027 1.86

ormulated to provide 300 mg/day monensin, 1000 lU/lb vitamin A, 0.1¾

sodium, and 0.15% added chlorine, 0. /% calcium, 0.7% potassium, and 10 ppm copper.

RESULTS;

Table XIV:

Feed intake and ruminal pH.

Control Flaxseed Flax/Lime SEM_ P value

Initial weight, lb 556.5 556.4 556;8 26.2 Q.7859

Feed intake (dry basis), lb 14.29 13.81 13.40 0.41 0.2032

Ruminal pH 7.00 7.02 7.04 0.069 0.8396 Docket No. 20480.203-PC

17

Table XV;

Fatty acid concentrations in plasma (pg/mi) of growing steers.

C18:0 122.6 148.9 130.1 125.8 1. 149.0 126.3 124.9 151.4 147.5 6.2 <0.0001 0.1586 0.315 C18: 1 76.8 92.0 83.1 80.8 87.3 86.5 80.1 79.4 86.4 4.8 0.1018 0.7244 0.346 C1 S:2 300.4 287.6 311.3 277,2 255.5 260.7 292.7 303.5 336.9 13.2 0.1791 O.0001 0.138 C18:3n³ 0.00 0.00 0.00 0.00 18,9 29.7 0.0 77,8 87.0 <0.0001 <0.0001 <0.00

Alpha-!ino!enic acid (C18:3n3; also commonly referred fo as ALA) is regarded as an essential nutrient for most animals, meaning that the body is incapable of synthesizing the fatty acid: in quantities sufficient to fulfill nutritional requirements of animals, thus indicating that it must be included as part of the animal's diet. This fatty acid is utilized as a precursor for synthesis of other important long-chain fatty acids, including eicosapentaenoic acid and docosahexaenoic acid (EPA and DBA), as well as in the synthesis of cholesterol, steroid hormones, eicosanoids, and other important compounds. This polyunsaturated fatty acid typically is subject to extensive biohydrogenatio (thus yielding stearic acid) by microorganisms within the rumen ecosystem, as taught by Montgomery et al, who have show that less than 5% of dietary ALA is available for absorption In the posfruminal digestive tract. See, Montgomery SP, Drouii!ard JS, Nagaraja TG, Titgemeyer EC, Sindi JJ., 2008, "Effects Of Supplemental Fat Source On Nutrient Digestion and Ruminal Fermentation In Steers^'"; J Anim Set; 86(3):640-5Q. Alpha-linolenic acid is present in immature cool-season grasses, legumes, and some forbs species, but is relatively deficient in mature forages, cereal grains, and many oilseeds. Flaxseed is a oilseed grown in temperate climates that is a rich source of alpha linolenic acid, containing approximately 40-45% oil, roughly 55-60% of which is in the form of ALA. Concentrations of linolenic acid i blood plasma are more~or~iess linearly associated with dietary concentrations of the fatty acid, thus making flaxseed an ideal candidate for evaluating efficacy of the method for protecting nutrients from the action of microorganisms within the forestomachs of ruminant animals.

Figure 2 of the drawings illustrates differences in blood concentrations of alpha- linolenic acid in animals fed different diets. During the pretrial period ail animals were fed a basal diet containing low levels of ALA, thus leading to low plasma concentrations of ALA in all treatment groups on Day 0 of the experiment. From day 1-14, all cattle were fed a common basal diet, but the flaxseed and flaxseed/lime treatment groups were supplemented with an equivalent amount of flaxseed in the unprotected and protected forms, respectively. On days 7 and 14 of the experiment, plasma concentrations of ALA remained low in the Control group, but increased sharply in the groups fed flaxseed. Moreover, compared to cattle fed the unprotected form of flaxseed, plasma concentrations of ALA were 412 and 292% greater for the Flaxseed/Ume treatment groups on days 7 and 14, respectively. These results clearly illustrate that the method was successful in rendering a greater proportion of the dietary ALA resistant to biohydfOgenatio by ruminal microorganisms.

As expected, fatty acid concentraitons among treatments were similar at day 0 of the experiment (prior to administration of dietary treatments). Differences among treatments were readily apparent after 7 and 4 days of supplementing the flaxseed and the prilled fla seed/iime mixture. Most notable are the elevated concentrations of alpha iinolenic acid {C18:3n3) for the prilled f!axseed/lime treatment, indicating that the process decreased susceptibility of the flaxseed to microbial biohydrogenation within the reticuio-rumen.

One particular advantage of the method of the invention might be referred to as the "self-healing" nature of the agglomerated particles which are produced in as far as their ability to protect core nutrients/compositions from degradation by ruminal microorganisms. Prior art products known to Applicant applied such things as fats (Baichenfs protected choline), synthetic polymers (Adisseo's protected lysine and methionine) or proteinaceous films to the surface of the core material, thus encasing the core materials and serving as a protective barrier. Ef icacy of these products is limited, however, due to the propensity for the outer shell to become fractured, thus exposing the core material to ruminal microorganisms, in the method of the present invention, a product is produced in the nature of a core material embedded within a matrix consisting of the carbonate/hydrate complex. Within the rumen, the material is exposed to relatively high concentrations of carbon dioxide, which further "re- carbonates" the surface to form an impervious outer layer. Fracturing of the prills is inevitabl during feed processing and as a result of masticatio by the animal. However, in the case of the method of the invention, the unprotected surfaces of fractured materials become carbonated through exposure to carbon dioxide in the rumen.

By creating a homogeneous or semi-homogeneous matrix, the present inventive method allows the intimate contact of active binder and coating material with the bypass material Hydrated lime of all forms will readily react with CC¾ to form calcium carbonate, i a wet C0₂ environment, such as the animal rumen, this reaction will proceed quickiy. Any surface that is alkaline due to the hydrate will react in these conditions, whether they are the outsides of non-coated prills, the surfaces in cracks or fresh surfaces brought about by degradation in handling or consumption. The formation of fresh caicium carbonate will passivate the surfaces and protect them from further ruminal degradation not only due to the creation of a chemically neutral surface, but also due to the increase in volume of the calcium compound as it recarbonates, The effect is somewhat like that achieved with doiomitic lime in construction applications. Doiomitic hydrated lime is specified for use in mortars and stuccos in earthquake zones due to its ability to recarbonate, fill in microcracks due to this volumetric expansion, and prevent the coalescing of these cracks Into big cracks that lead to failures. The method of the present invention thus uses a special hydrated lime binder to create a matrix wit an ability to repair defects while in the animal rumen, an effect not achieved with the products of the prior art, Additionally, any of the binder that does abrade, break off or dissolve will provide positive rumen buffering.

While the invention has been described in several preferred forms, those skilled in the relevant arts will recognize that various modifications can be made while still falling within the scope of the invention as defined in the claims which follow. For example, the controlling parameters of these manufacturing processes can be modified or altered to adjust the finished characteristics of the agglomerated¹ particles. Those characteristics whic can be modified Include, but are not necessary limited to, the particles apparent density, particle size, particle porosity all of which can impart or retard: certain characteristics which are deemed beneficial or detrimental to their use as discussed in the body of this invention. Additional control of the finished materials' characteristics may be modified by a secondary coaling or a layering of a secondary coating.

When fed to ruminants, the particles are exposed to the aqueous, C0₂~ric environment of the rumen, and: chemical hydrates on the surface of the particle are recarbonated to form CaCQs, iVigCQg, or other chemical carbonates, which are substantially resistant to degradation within the rumen. The re-carbonated surface serves as an effective barrier to microorganisms, preventing access to feed ingredients or other components imbedded within the agglomerated particles. The agglomerated particles, or fragments thereof, are passed: from the rumen, through the omasum, and into the abomasum where they are exposed to gastric hydrochloric acid secretions. In the presence of hydrochloric acid the carbonates are dissolved, releasing the feed ingredients or other components embedded therein. Components released from the matrix are then available for digestion and absorption or other actions in the post-rurninai digestive tract.

As has been explained, the preferred process utilizes mineral hydrates (hydroxides) as the binder for the matrix-forming materials. Where it may be suitable to release some proportion of the agglomerated material within the rumen, the matrix would be presented to the animal in its hydrated (or partially hydrated) form without prior re~ carbonation, thu depending on the ruminal environment to generate a protective carbonate laye on the particle surface, and in so doing releasing a portion of the matrix material. Where it is desired to minimize release of materials within the rumen, hydrates may be exposed to carbon dioxide during the manufacturing to yield products that contain a greater proportion of mineral carbonates that are more-or- iess ruminaliy inert. As an alternative, it is conceivable to utilize carbonates directly for preparation of the matrix.

The process is suitable for increasing the proportion of ^'dietary ingredients presented for digestion and absorption withi the post-ruminal digestive tract by inhibiting premature digestion by microorganisms inhabiting the rumen. The method can be applied: to lysine, methionine, or other amino acids as a means of increasing the proportion of these compounds that are available for absorption in the postruminai tract, thus improving nutritional status of the host.

Aluminum compounds may also be included in the binder compositions in some cases.

Similarly, the process can be applied for choline and/or water soluble vitamins, vitamins, including ascorbic acid (vitami C), vitamin including^; Bi (thiamine), B₂ (riboflavin), B₃ (niacin or niacinamide), B₅ (pantothenic acid), Β_δ (pyridoxine, pyridoxal, or pyridoxamine, or pyridoxine hydrochloride), B₇ (biotin), B_s (folic acid), and B₁₂ (cobalamins; commonly cyanocobaiamin), ail of which are highly susceptible to extensive hydrolysis by ruminai microorganisms, and that may be required by the host animal in quantities that exceed those which normally escape digestion by ruminai microbes.

The method also has application for the protection of monounsaturated or polyunsaturated lipids, which normally are extensively biohydrcgenated by ruminai microorganisms to yield saturated lipids. Complexing lipids in the manner described herein decreases the extent of biohydrogenation of unsaturated fatty acids, thereby making it feasible to increase the proportion of unsaturated fats i meat, milk, and animal fats. As examples, animal products can be enriched with omega-3 fatty acids, conjugated linoleic acids, or other fatty acids deemed useful as nutrients for humans and other animals. As a further consideration, unsaturated fats and derivatives thereof may be toxic to ruminai microorganisms, and when present in excess can decrease digestion of other components of the diet, especially fiber. Complexing lipids usin the method described: herein avoids interaction between lipids and ruminai microorganisms, thus maintaining more optimal digestion of fibrous feeds and other ingredients that may otherwise be impaired in the presence of unsaturated lipids. In the postruminai digestive track polyunsaturated fats generally are more digestible than saturated: fats, thus yielding more energy for the animal. Preventing extensive biohydrogenation of lipids thus represents a means of improving energy value of fats for ruminants. Mineral elements also constitute a logical target for protection. For example, sodium se!enite, which is a relatively available source of essential selenium, is utilized by ruminal microorganism to synthesize selenocysteine, which has relatively poor bioavailability in the post-ruminal digestive tract. Protecting selenium within the mineral matrix precludes interaction with ruminal microbes, preserving the more available form of this essential mineral. Minimizing interactions between mineral elements and ruminal microorganisms may have other advantages, as well. For instance, heavy metals such as zinc, copper, and¹ manganes are capable of inducing antimicrobial resistance among microorganisms exposed to these elements, thus impacting efficacy of important antimicrobial drugs. By embedding the heavy metals within a protective matrix, interaction with ruminal microorganisms are avoided, thus precluding the necessity for microorganisms to transcribe genes that encode for antimicrobial resistance elements.

The applications above are intended to serve on!y as examples, and by no means should these be construed as a finite iist of applications. The same process could be employed as a means of protecting fat soluble vitamins, enzymes, probiotics, prebiotics, carbohydrates, pharmaceuticals, essential oils, minerals, and other compounds, thus assuring that greater proportions of these products are presented post-rumina!ly to enhance their desired effects on the host animal or microbial populations in the postruminal tract

Thus, while the invention has been shown in several of its forms, it is not thus iimited but is susceptible to various changes and modifications without departing from the spirit thereof.

Claims

Claims;

1. A method for processing ruminant animal feed which increases the proportion of dietary ingredients present in the feed that are resistant to degradation by rumina! microorganisms, the method comprising the steps of: combining ruminant animal feed ingredients with a binder composition and a blending aid to thereby form a raw feed mixture; processing the raw feed mixture so formed into a pellet or prill comprised of agglomerated particles; and wherein the binder composition is comprised of a calcific or dolomitic mineral hydrate, either alone or in combination with a companion composition selected from the group consisting of mineral carbonates, mineral oxides, and combinations thereof.

2. The method of Claim 1., wherei the binder composition is made u of hyd rated lime combined with a mineral carbonate.

3, The method of Claim 2, wherein the hydrated lime is selected from the group consisting of high calcium, doiomitic or partially hydrated dolomitic limes produced in a pressure hydrator or in an atmospheric hydrator.

4. The method of Claim 2, wherein the hydrated limes are made from a starting materiar selected from the group consisting of high calcium lime, magnesium lime, calcific dolomitic lime and doiomitic lime.

5. The method of Claim 1 , wherein the raw feed mixture is processed by means of a pin mixer, peiiet mill, disc pelletizer, drum pelletizer, extruder, or other device suitable for producing agglomerated particles.

6. The method of Claim 1 , wherein the blending aid is water.

7. The method of Claim 1 , wherein the blending aid is a hig moisture content ingredient which contains water.

8. The method of Claim 1 , wherein the blending aid is a non-aqueous solvent.

9. The method of Claim 1 , wherein the binder composition is comprised of a mixture of hydrated lime and a calcific or dolomitic carbonate mineral material.

1.0. Th method of Claim 9. wherein the carbonate mineral material is selected from the group consisting of calcium carbonate, magnesium carbonate, dolomite and mixtures thereof.

11. The method of Claim 1 , wherein the binder composition is about 40% by weight hydrated lime and 60% by weight dolomitic limestone,

12. The method of Claim 1, wherein the agglomerated particles have a secondar coating applied after agglomeration.

13. The method of Claim 1.2, wherein the agglomerated particles are exposed to carbon dioxide during processing to thereby yield a final product which contains a greater proportion of mineral carbonates which are basically ruminai!y inert.

14. The method of Claim 1, wherein the agglomerated particles include lysine, methionine or other amino acids as a means of increasing the proportion of those compounds that are available for absorption in the animal postrum^'inal tract.

15. The method of Claim 1 , wherein the agglomerated particles include choline and water soluble vitamins that may be required by the animal in quantities that exceed those whic would normally escape digestion by ruminai microbes.

1.6. The method of Claim 1 , wherein the agglomerated particles so produced provide for the protection of rnonounsaturate or polyunsaturated lipids whic normally are extensively biOhydrogenated^ by ruminai microorganisms to yield saturated lipids.

17. The method of Claim 1 , wherein the agglomerated particles so produced provide for the protection of fat soluble vitamins, enzymes, probiotics, prebiotics, carbohydrates, pharmaceuticals, essential oils, minerals, and other compounds which assure that a greater proportion of these products are presented post- ruminaSiy.

18. The method of Claim 2, wherein the hydrated lime is present in the range from about 10 to 95% by weight of the total raw feed mixture.

19. A ruminant animal feed, comprising: ruminant animal feed ingredients combined with a binder composition and water to thereby form a raw feed mixture, the raw feed mixture so formed being processed into a processed feed pellet comprised of agglomerated particles; wherein the binder composition is comprised of a calcific or dolomitic mineral hydrate, either alone or in combination with a companion composition selected from the group consisting of mineral carbonates, mineral oxides, and combinations thereof; and wherein the so processed agglomerated particles are effective to increase the proportion of dietary ingredients present in the feed that are resistant to degradation by ruminai microorganisms.

20. The ruminant animal feed of Claim 19, wherein the binder compositio is comprised of dolomitic hydrated lime combined with a companion material selected from the group consisting of calcium carbonate, magnesium carbonate, dolomite and mixtures thereof.