WO2013113699A3 - Hyperthermophilic polymerase enabled proximity extension assay - Google Patents

Hyperthermophilic polymerase enabled proximity extension assay Download PDF

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Publication number
WO2013113699A3
WO2013113699A3 PCT/EP2013/051690 EP2013051690W WO2013113699A3 WO 2013113699 A3 WO2013113699 A3 WO 2013113699A3 EP 2013051690 W EP2013051690 W EP 2013051690W WO 2013113699 A3 WO2013113699 A3 WO 2013113699A3
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Prior art keywords
polymerase
proximity
analyte
assay
nucleic acid
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Ceased
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PCT/EP2013/051690
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French (fr)
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WO2013113699A2 (en
Inventor
Simon Fredriksson
Martin LUNDBERG
Anna Eriksson
Emma RENNEL-DICKENS
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Olink Holding AB
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Olink AB
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Priority to CN201380007279.XA priority Critical patent/CN104114718B/en
Priority to EP13703352.8A priority patent/EP2809804B1/en
Priority to US14/375,236 priority patent/US9902993B2/en
Publication of WO2013113699A2 publication Critical patent/WO2013113699A2/en
Publication of WO2013113699A3 publication Critical patent/WO2013113699A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a proximity probe based detection assay ("proximity assay") for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific "background" signals, wherein the improvement comprises the use in such assays of a hyperthermophilic polymerase, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) extending the 3' end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the extension reaction comprises increasing the temperature of assay above room temperature and uses a polymerase enzyme which is characterised as having less than 20% of its maximal enzyme activity at 40°C and having less than 10% of its maximal enzyme activity at 25°C, wherein the optimum temperature for maximal activity of the polymerase is more than 40°C and wherein the polymerase is selected from Pyrococcus furiosus (Pfu) DNA polymerase and Pyrococcus woesei (Pwo) DNA polymerase or a derivative or mutant thereof, preferably wherein said derivative is a sequence- modified derivative; and (d) amplifying and detecting the extension product.
PCT/EP2013/051690 2012-01-30 2013-01-29 Hyperthermophilic polymerase enabled proximity extension assay Ceased WO2013113699A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201380007279.XA CN104114718B (en) 2012-01-30 2013-01-29 Proximity extension assays enabled by hyperthermophilic polymerases
EP13703352.8A EP2809804B1 (en) 2012-01-30 2013-01-29 Hyperthermophilic polymerase enabled proximity extension assay
US14/375,236 US9902993B2 (en) 2012-01-30 2013-01-29 Hyperthermophilic polymerase enabled proximity extension assay

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1201547.5 2012-01-30
GBGB1201547.5A GB201201547D0 (en) 2012-01-30 2012-01-30 Method and product

Publications (2)

Publication Number Publication Date
WO2013113699A2 WO2013113699A2 (en) 2013-08-08
WO2013113699A3 true WO2013113699A3 (en) 2014-03-20

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PCT/EP2013/051690 Ceased WO2013113699A2 (en) 2012-01-30 2013-01-29 Hyperthermophilic polymerase enabled proximity extension assay

Country Status (5)

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US (1) US9902993B2 (en)
EP (1) EP2809804B1 (en)
CN (1) CN104114718B (en)
GB (1) GB201201547D0 (en)
WO (1) WO2013113699A2 (en)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904181A1 (en) * 2013-03-13 2014-10-09 Anahit Aghvanyan Sandwich immunoassay comprising anchoring reagent
EP2971121A4 (en) 2013-03-15 2016-11-30 Fluidigm Corp SIMULTANEOUS DETECTION OF TARGET PROTEIN AND TARGET NUCLEIC ACIDS IN A SINGLE CELL
EP3041957A4 (en) * 2013-09-04 2017-03-29 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
EP3143401A4 (en) 2014-05-15 2017-10-11 Meso Scale Technologies, LLC Improved assay methods
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
FR3024465B1 (en) 2014-07-30 2018-03-23 Biomerieux CHARACTERIZATION OF MICROORGANISMS BY MALDI-TOF
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
EP4108781A1 (en) 2016-05-27 2022-12-28 President and Fellows of Harvard College Conditional primer extension for single-molecule detection
AU2017302300B2 (en) 2016-07-27 2023-08-17 The Board Of Trustees Of The Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US20180119202A1 (en) * 2016-11-01 2018-05-03 University Of Washington Proximity-enhanced nucleic acid-amplified protein detection
EP3580351A1 (en) 2017-02-13 2019-12-18 Qiagen Sciences, LLC Polymerase enzyme from 9°n
WO2019238944A1 (en) 2018-06-15 2019-12-19 Olink Proteomics Ab Biomarker panel for ovarian cancer
US11421216B2 (en) 2018-12-21 2022-08-23 Illumina, Inc. Nuclease-based RNA depletion
CN120098057A (en) 2019-03-01 2025-06-06 中尺度技术有限责任公司 Electrochemiluminescent labeled probes for use in immunological methods, methods of using such probes, and kits containing such probes
CN114616344B (en) 2019-10-28 2026-03-31 普赛尔根技术股份公司 Methods for plotting rolling circle amplification products
EP4069731A4 (en) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. NUCLEIC ACID-COUPLED IMMUNE SANDWICH ASSAY (NULISA)
GB202004472D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Method for detecting analytes of varying abundance
WO2021191442A1 (en) 2020-03-27 2021-09-30 Olink Proteomics Ab Method for detecting analytes of varying abundance
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
CN115552028A (en) * 2020-05-29 2022-12-30 美国西门子医学诊断股份有限公司 Method of detecting an analyte in a sample
WO2022051872A1 (en) * 2020-09-14 2022-03-17 Mcmaster University Electrochemical biosensor for target analyte detection
GB202017873D0 (en) 2020-11-12 2020-12-30 Olso Unversitetssykehus Hf Method of determining DLBCL prognosis
GB202018503D0 (en) 2020-11-25 2021-01-06 Olink Proteomics Ab Analyte detection method employing concatamers
EP4303315B1 (en) * 2021-03-30 2024-11-06 Pixelgen Technologies Ab Spatial mapping by serial primer extension
CN114277090A (en) * 2021-12-17 2022-04-05 宁波熙宁检测技术有限公司 Detection method and detection kit for AAV8 neutralizing antibody
EP4615996A1 (en) 2022-11-11 2025-09-17 Olink Proteomics AB Library of proximity probes and method of use
EP4382534A1 (en) 2022-12-06 2024-06-12 Biomérieux Synthetic peptide for use in an immunomolecular assay
WO2025012398A1 (en) 2023-07-11 2025-01-16 Olink Proteomics Ab Dried reagents
WO2025012226A1 (en) 2023-07-12 2025-01-16 Olink Proteomics Ab Method, components and software for detecting a systematic error in a protein detection system
WO2025031642A1 (en) 2023-08-07 2025-02-13 Olink Proteomics Ab A method for determining run errors for an analysis-method for quantifying a plurality of analytes in a plurality of biological samples
CN117821412B (en) * 2023-12-15 2025-02-18 湖北大学 A dCE-KOD DNA polymerase and its preparation method and application
WO2025199005A1 (en) 2024-03-20 2025-09-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biomarkers for identifying cancer patients at high risk for chemotherapy-induced cardiotoxicity
WO2025252658A1 (en) 2024-06-03 2025-12-11 Olink Proteomics Ab New product and method
WO2025262178A1 (en) * 2024-06-18 2025-12-26 Olink Proteomics Ab System and method for detecting analytes in a sample

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
WO2006137932A2 (en) * 2004-11-03 2006-12-28 Leucadia Technologies, Inc. Homogeneous analyte detection
WO2007044864A2 (en) * 2005-10-11 2007-04-19 Stratagene California Target nucleic acid signal detection
WO2007107743A1 (en) * 2006-03-20 2007-09-27 Olink Ab Method for analyte detection using proximity probes
WO2012007511A1 (en) * 2010-07-15 2012-01-19 Olink Ab Blocking reagent and methods for the use thereof
WO2012104261A1 (en) * 2011-01-31 2012-08-09 Olink Ab Exonuclease enabled proximity extension assays

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU530410B2 (en) 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5266684A (en) 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
US5733523A (en) 1990-12-10 1998-03-31 Akzo Nobel N.V. Targeted delivery of a therapeutic entity using complementary oligonucleotides
CA2119126C (en) 1991-09-16 1996-09-03 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes
US5639603A (en) 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5770358A (en) 1991-09-18 1998-06-23 Affymax Technologies N.V. Tagged synthetic oligomer libraries
WO1993015226A1 (en) 1992-01-28 1993-08-05 President And Fellows Of Harvard College Method for nucleic acid hybridization using single-stranded dna binding protein
US5321130A (en) 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5541061A (en) 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
AU687010B2 (en) 1992-07-17 1998-02-19 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5288514A (en) 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5565324A (en) 1992-10-01 1996-10-15 The Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5721099A (en) 1992-10-01 1998-02-24 Trustees Of Columbia University In The City Of New York Complex combinatorial chemical libraries encoded with tags
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5658751A (en) 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5440016A (en) 1993-06-18 1995-08-08 Torrey Pines Institute For Molecular Studies Peptides of the formula (KFmoc) ZZZ and their uses
US5851829A (en) 1993-07-16 1998-12-22 Dana-Farber Cancer Institute Method of intracellular binding of target molecules
US5593853A (en) 1994-02-09 1997-01-14 Martek Corporation Generation and screening of synthetic drug libraries
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5688997A (en) 1994-05-06 1997-11-18 Pharmacopeia, Inc. Process for preparing intermediates for a combinatorial dihydrobenzopyran library
US5525735A (en) 1994-06-22 1996-06-11 Affymax Technologies Nv Methods for synthesizing diverse collections of pyrrolidine compounds
US5549974A (en) 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5463564A (en) 1994-09-16 1995-10-31 3-Dimensional Pharmaceuticals, Inc. System and method of automatically generating chemical compounds with desired properties
US5688696A (en) 1994-12-12 1997-11-18 Selectide Corporation Combinatorial libraries having a predetermined frequency of each species of test compound
WO1997000887A1 (en) 1995-06-21 1997-01-09 Martek Biosciences Corporation Combinatorial libraries of labeled biochemical compounds and methods for producing same
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5731423A (en) 1996-03-21 1998-03-24 Transcell Technologies, Inc. Process for preparing sulfoxides
US6117635A (en) 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6183997B1 (en) 1997-03-21 2001-02-06 Stratagene Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
WO1998054312A1 (en) 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
US5989823A (en) 1998-09-18 1999-11-23 Nexstar Pharmaceuticals, Inc. Homogeneous detection of a target through nucleic acid ligand-ligand beacon interaction
EP0930370B1 (en) 1997-12-15 2007-08-08 CSL Behring GmbH Labeled primer for use in detection of target nucleic acids
AU3601599A (en) 1998-03-25 1999-10-18 Ulf Landegren Rolling circle replication of padlock probes
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
SE516272C2 (en) 2000-02-18 2001-12-10 Ulf Landegren Methods and kits for analyte detection using proximity probing
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
JP2005509444A (en) 2001-11-23 2005-04-14 シモン・フレデリックソン Methods and kits for proximity probing with multivalent proximity probes
US10669569B2 (en) 2010-10-15 2020-06-02 Navinci Diagnostics Ab Dynamic range methods

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
WO2006137932A2 (en) * 2004-11-03 2006-12-28 Leucadia Technologies, Inc. Homogeneous analyte detection
US20080131883A1 (en) * 2004-11-03 2008-06-05 Iris Molecular Diagnostics, Inc. Homogeneous Analyte Detection
WO2007044864A2 (en) * 2005-10-11 2007-04-19 Stratagene California Target nucleic acid signal detection
WO2007107743A1 (en) * 2006-03-20 2007-09-27 Olink Ab Method for analyte detection using proximity probes
WO2012007511A1 (en) * 2010-07-15 2012-01-19 Olink Ab Blocking reagent and methods for the use thereof
WO2012104261A1 (en) * 2011-01-31 2012-08-09 Olink Ab Exonuclease enabled proximity extension assays

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
D. A. DI GIUSTO: "Proximity extension of circular DNA aptamers with real-time protein detection", NUCLEIC ACIDS RESEARCH, vol. 33, no. 6, 23 March 2005 (2005-03-23), pages e64 - e64, XP055096915, DOI: 10.1093/nar/gni063 *
M. LUNDBERG ET AL: "Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood", NUCLEIC ACIDS RESEARCH, vol. 39, no. 15, 6 June 2011 (2011-06-06), pages e102 - e102, XP055022762, ISSN: 0305-1048, DOI: 10.1093/nar/gkr424 *

Also Published As

Publication number Publication date
EP2809804B1 (en) 2016-04-06
CN104114718B (en) 2018-02-23
GB201201547D0 (en) 2012-03-14
US20150044674A1 (en) 2015-02-12
EP2809804A2 (en) 2014-12-10
WO2013113699A2 (en) 2013-08-08
CN104114718A (en) 2014-10-22
US9902993B2 (en) 2018-02-27

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