WO2013134174A3 - Recombinant bacteria comprising novel sucrose transporters - Google Patents

Recombinant bacteria comprising novel sucrose transporters Download PDF

Info

Publication number
WO2013134174A3
WO2013134174A3 PCT/US2013/028970 US2013028970W WO2013134174A3 WO 2013134174 A3 WO2013134174 A3 WO 2013134174A3 US 2013028970 W US2013028970 W US 2013028970W WO 2013134174 A3 WO2013134174 A3 WO 2013134174A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombinant bacteria
sucrose
recombinant
polypeptide
sucrose transporters
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/028970
Other languages
French (fr)
Other versions
WO2013134174A2 (en
Inventor
Kristin Ruebling-Jass
Jean-Francois Tomb
Tina K. Van Dyk
Zheng YOU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to AU2013230162A priority Critical patent/AU2013230162A1/en
Priority to JP2014561008A priority patent/JP2015509379A/en
Priority to CN201380012400.8A priority patent/CN104302661A/en
Priority to KR1020147027717A priority patent/KR20140132392A/en
Priority to SG11201405338YA priority patent/SG11201405338YA/en
Priority to IN6652DEN2014 priority patent/IN2014DN06652A/en
Priority to EP13709706.9A priority patent/EP2822960A2/en
Priority to US14/382,343 priority patent/US20150064755A1/en
Priority to CA2865014A priority patent/CA2865014A1/en
Publication of WO2013134174A2 publication Critical patent/WO2013134174A2/en
Publication of WO2013134174A3 publication Critical patent/WO2013134174A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/335—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Lactobacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01007—Sucrose phosphorylase (2.4.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01001—Hexokinase (2.7.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01003—Ketohexokinase (2.7.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01004—Fructokinase (2.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
PCT/US2013/028970 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters Ceased WO2013134174A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU2013230162A AU2013230162A1 (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
JP2014561008A JP2015509379A (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising a novel sucrose transporter
CN201380012400.8A CN104302661A (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
KR1020147027717A KR20140132392A (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
SG11201405338YA SG11201405338YA (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
IN6652DEN2014 IN2014DN06652A (en) 2012-03-05 2013-03-05
EP13709706.9A EP2822960A2 (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
US14/382,343 US20150064755A1 (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters
CA2865014A CA2865014A1 (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/412,193 2012-03-05
US13/412,193 US9017961B2 (en) 2012-03-05 2012-03-05 Recombinant bacteria comprising novel sucrose transporters

Publications (2)

Publication Number Publication Date
WO2013134174A2 WO2013134174A2 (en) 2013-09-12
WO2013134174A3 true WO2013134174A3 (en) 2013-12-27

Family

ID=47884600

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2013/028970 Ceased WO2013134174A2 (en) 2012-03-05 2013-03-05 Recombinant bacteria comprising novel sucrose transporters

Country Status (10)

Country Link
US (3) US9017961B2 (en)
EP (1) EP2822960A2 (en)
JP (1) JP2015509379A (en)
KR (1) KR20140132392A (en)
CN (1) CN104302661A (en)
AU (1) AU2013230162A1 (en)
CA (1) CA2865014A1 (en)
IN (1) IN2014DN06652A (en)
SG (1) SG11201405338YA (en)
WO (1) WO2013134174A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ602504A (en) 2008-11-18 2014-01-31 Beth Israel Hospital Antiviral vaccines with improved cellular immunogenicity
US9688967B2 (en) 2014-12-05 2017-06-27 Synlogic, Inc. Bacteria engineered to treat diseases associated with hyperammonemia
WO2016183532A1 (en) * 2015-05-13 2016-11-17 Synlogic, Inc. Bacteria engineered to treat a disease or disorder
US11883439B2 (en) 2015-05-13 2024-01-30 Synlogic Operating Company, Inc. Bacteria engineered to treat a disease or disorder
ES2881384T3 (en) 2015-05-13 2021-11-29 Synlogic Operating Co Inc Engineered bacteria to reduce hyperphenylalaninemia
ES2925049T3 (en) 2015-11-16 2022-10-13 Synlogic Operating Co Inc Engineered bacteria to reduce hyperphenylalaninemia
CN107058205B (en) * 2017-06-05 2020-05-08 江南大学 Recombinant bacillus subtilis for producing sucrose phosphorylase and application thereof
CN111655841A (en) 2017-06-21 2020-09-11 同生运营公司 bacteria used to treat disorders
CN108070579A (en) * 2017-12-21 2018-05-25 中诺生物科技发展江苏有限公司 A kind of invertase industrialized preparing process
CN108504616B (en) * 2018-04-03 2020-05-29 广西科学院 A kind of recombinant clostridium beijerinckii efficiently fermenting sucrose and method for improving sucrose fermentation performance of clostridium beijerinckii
KR102201720B1 (en) * 2018-05-24 2021-01-12 한화솔루션 주식회사 Recombinant Corynebacterium Producing 1,3-PDO and Inhibited 3-HP Production and Method for Preparing 1,3-PDO Using Thereof
BR112022018571A2 (en) 2020-03-20 2022-11-01 Synlogic Operating Co Inc MICRO-ORGANISMS MANIPULATED TO REDUCE HYPERPHENYLALANINEMIA
CN117947025B (en) * 2023-11-07 2025-06-10 华南农业大学 Yellow beam wood sucrose transporter NcSUT gene promoter and application thereof
CN118086444B (en) * 2024-04-28 2024-06-28 山东德普新材料科技有限公司 Method for co-producing 1, 3-propanediol and 3-hydroxypropionic acid through fermentation

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010049126A1 (en) * 2000-04-26 2001-12-06 Ajinomoto Co., Inc. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid
US20110136190A1 (en) * 2009-12-04 2011-06-09 E. I. Du Pont De Nemours And Company Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose
WO2013025286A1 (en) * 2011-08-16 2013-02-21 E. I. Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5686276A (en) 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
JP4327909B2 (en) 1996-11-13 2009-09-09 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー Method for producing 1,3-propanediol by recombinant organisms
WO1999028480A1 (en) 1997-12-02 1999-06-10 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
BRPI0013315B8 (en) 1999-08-18 2018-02-27 Du Pont isolated nucleic acid fragment, polypeptide, chimeric gene, microorganism, recombinant microorganism, e.g. recombinant coli, klp23 strain of e.g. recombinant coli, strain rj8 of e.g. recombinant coli, pdt29 vector, pkp32 vector and 1,3-propanediol bioproduction processes
EP2374879A3 (en) 2002-04-22 2012-01-11 E. I. du Pont de Nemours and Company Promoter and plasmid system for genetic engineering
WO2004033646A2 (en) 2002-10-04 2004-04-22 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
US7524660B2 (en) 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
ES2424240T3 (en) 2007-12-18 2013-09-30 Korea Advanced Institute Of Science And Technology Recombinant microorganism with the ability to use sucrose as a carbon source
MX2011004842A (en) 2008-11-07 2011-05-30 Metabolic Explorer Sa Use of sucrose as substrate for fermentative production of 1,2-propanediol.
US20110144377A1 (en) 2009-06-16 2011-06-16 E. I. Du Pont Nemours And Company Process for the biological production of 3-hydroxypropionic acid with high yield
US8129170B1 (en) 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8222000B2 (en) 2010-12-06 2012-07-17 E I Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US8686114B2 (en) 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010049126A1 (en) * 2000-04-26 2001-12-06 Ajinomoto Co., Inc. Amino acid producing strains belonging to the genus Escherichia and method for producing amino acid
US20110136190A1 (en) * 2009-12-04 2011-06-09 E. I. Du Pont De Nemours And Company Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose
WO2013025286A1 (en) * 2011-08-16 2013-02-21 E. I. Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
DATABASE UniProt [online] 1 March 2003 (2003-03-01), "SubName: Full=Sucrose transport protein;", XP002713334, retrieved from EBI accession no. UNIPROT:Q8G7X9 Database accession no. Q8G7X9 *
DATABASE UniProt [online] 10 May 2005 (2005-05-10), "SubName: Full=Sugar transport protein;", XP002713332, retrieved from EBI accession no. UNIPROT:Q564C6 Database accession no. Q564C6 *
DATABASE UniProt [online] 22 February 2012 (2012-02-22), "SubName: Full=Oligosaccharide:H+ symporter;", XP002705617, retrieved from EBI accession no. UNIPROT:G9SK52 Database accession no. G9SK52 *
DATABASE UniProt [online] 23 January 2007 (2007-01-23), "SubName: Full=Sucrose transport protein;", XP002713335, retrieved from EBI accession no. UNIPROT:A1A2J7 Database accession no. A1A2J7 *
DATABASE UniProt [online] 26 May 2009 (2009-05-26), "SubName: Full=Sucrose transporter;", XP002705616, retrieved from EBI accession no. UNIPROT:C1M5D2 Database accession no. C1M5D2 *
DATABASE UniProt [online] 9 February 2010 (2010-02-09), "SubName: Full=Sucrose transport protein;", XP002713333, retrieved from EBI accession no. UNIPROT:D1NSR6 Database accession no. D1NSR6 *
JAHREIS KNUT ET AL: "Adaptation of sucrose metabolism in the Escherichia coli wild-type strain EC3132.", JOURNAL OF BACTERIOLOGY OCT 2002, vol. 184, no. 19, October 2002 (2002-10-01), pages 5307 - 5316, XP002705618, ISSN: 0021-9193 *
JANER C ET AL: "Hydrolysis of Oligofructoses by the Recombinant beta-Fructofuranosidase from Bifidobacterium lactis", SYSTEMATIC AND APPLIED MICROBIOLOGY, URBAN & FISCHER, AMSTERDAM, NL, vol. 27, no. 3, 1 January 2004 (2004-01-01), pages 279 - 285, XP004957499, ISSN: 0723-2020, DOI: 10.1078/0723-2020-00274 *
MONICA M OLSON ET AL: "Production of tyrosine from sucrose or glucose achieved by rapid genetic changes to phenylalanine-producing Escherichia coli strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 74, no. 5, 11 January 2007 (2007-01-11), pages 1031 - 1040, XP019489541, ISSN: 1432-0614, DOI: 10.1007/S00253-006-0746-2 *
SCHELL M A ET AL: "The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES - PNAS, NATIONAL ACADEMY OF SCIENCES, US, vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14422 - 14427, XP002313989, ISSN: 0027-8424, DOI: 10.1073/PNAS.212527599 *
TSUNEKAWA H ET AL: "ACQUISITION OF A SUCROSE UTILIZATION SYSTEM IN ESCHERICHIA COLI K-12 DERIVATIVES AND ITS APPLICATION TO INDUSTRY", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 58, no. 6, 1 June 1992 (1992-06-01), pages 2081 - 2088, XP001024474, ISSN: 0099-2240 *

Also Published As

Publication number Publication date
SG11201405338YA (en) 2014-09-26
KR20140132392A (en) 2014-11-17
US9017961B2 (en) 2015-04-28
CA2865014A1 (en) 2013-09-12
IN2014DN06652A (en) 2015-05-22
CN104302661A (en) 2015-01-21
US20130230893A1 (en) 2013-09-05
EP2822960A2 (en) 2015-01-14
AU2013230162A1 (en) 2014-08-28
US20150064755A1 (en) 2015-03-05
US20150203876A1 (en) 2015-07-23
JP2015509379A (en) 2015-03-30
WO2013134174A2 (en) 2013-09-12

Similar Documents

Publication Publication Date Title
WO2013134174A3 (en) Recombinant bacteria comprising novel sucrose transporters
WO2014118360A3 (en) Carbohydrate degrading polypeptide and uses thereof
WO2012061530A8 (en) Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
PH12014501726B1 (en) Glyphosate resistant plants and associated methods
MY160897A (en) Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
WO2014202616A3 (en) Rasamsonia gene and use thereof
HK1207880A1 (en) Improved enzyme variants
MX2012015142A (en) Polypeptide having beta-glucosidase activity and uses thereof.
EA201300455A1 (en) POLYPEPTIDES WITH PERMAIA ACTIVITY
WO2010133644A3 (en) Amylase polypeptides
WO2014020143A3 (en) Use of a xylanase in corn and corn by-products
WO2014202622A3 (en) Rasamsonia gene and use thereof
CA2863578C (en) Methods and compositions for increasing sialic acid production and treating sialic related disease conditions
MX2015001542A (en) Method.
WO2013016724A3 (en) Decarboxylase proteins with high keto-isovalerate decarboxylase activity
AR087885A1 (en) USEFUL ENZYMES FOR THE FOOD INDUSTRY
MX2012009847A (en) Modified family 5 cellulases and uses thereof.
WO2013189972A3 (en) Use of polypeptides having protease activity in animal feed and detergents
WO2014036806A8 (en) Glyphosate-tolerant gene and an application thereof
WO2015040609A8 (en) Erk-derived peptides and uses thereof
IN2015DN02650A (en)
WO2012067933A3 (en) Recombinant escherichia coli having enhanced acetyl-coenzyme a synthetase activity for producing glyerol and glycerol-derived products
JP2013512675A5 (en)
WO2011152568A8 (en) A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family, having attenuated expression of genes encoding a lysine/arginine/ornithine transporter
WO2014078213A3 (en) Modified apol1 polypeptides

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13709706

Country of ref document: EP

Kind code of ref document: A2

REEP Request for entry into the european phase

Ref document number: 2013709706

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2013709706

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2865014

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2013230162

Country of ref document: AU

Date of ref document: 20130305

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 2014561008

Country of ref document: JP

Kind code of ref document: A

ENP Entry into the national phase

Ref document number: 20147027717

Country of ref document: KR

Kind code of ref document: A

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112014021896

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112014021896

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20140904