WO2014136076A2 - Assembly comprising an absorber of near infrared (nir) light covalently linked to an inhibitor of carbonic anhydrase - Google Patents

Assembly comprising an absorber of near infrared (nir) light covalently linked to an inhibitor of carbonic anhydrase Download PDF

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WO2014136076A2
WO2014136076A2 PCT/IB2014/059490 IB2014059490W WO2014136076A2 WO 2014136076 A2 WO2014136076 A2 WO 2014136076A2 IB 2014059490 W IB2014059490 W IB 2014059490W WO 2014136076 A2 WO2014136076 A2 WO 2014136076A2
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assembly according
nir
absorber
near infrared
linker
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WO2014136076A3 (en
Inventor
Roberto Pini
Fulvio RATTO
Francesca TATINI
Franco Fusi
Sonia CENTI
Andrea Scozzafava
Claudiu Trandafir SUPURAN
Fabrizio Carta
Sergio Capaccioli
Ewa Janina WITORT
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Consiglio Nazionale delle Richerche CNR
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Consiglio Nazionale delle Richerche CNR
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Priority to EP14714382.0A priority Critical patent/EP2964272A2/en
Priority to US14/773,046 priority patent/US20160015661A1/en
Publication of WO2014136076A2 publication Critical patent/WO2014136076A2/en
Publication of WO2014136076A3 publication Critical patent/WO2014136076A3/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
    • A61K41/0052—Thermotherapy; Hyperthermia; Magnetic induction; Induction heating therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
    • A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/22—Echographic preparations; Ultrasonic imaging preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/22—Echographic preparations; Ultrasonic imaging preparations
    • A61K49/221—Echographic preparations; Ultrasonic imaging preparations characterised by the targeting agent or modifying agent linked to the acoustically-active agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/22—Echographic preparations; Ultrasonic imaging preparations
    • A61K49/222—Echographic preparations; Ultrasonic imaging preparations characterised by a special physical form, e.g. emulsions, liposomes
    • A61K49/225—Microparticles, microcapsules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/062—Photodynamic therapy, i.e. excitation of an agent
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy
    • A61N5/06—Radiation therapy using light
    • A61N5/0613—Apparatus adapted for a specific treatment
    • A61N5/0625—Warming the body, e.g. hyperthermia treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • the present invention refers to the pharmaceutical field and in particular to an assembly comprising an absorber of near infrared (NIR) light covalently linked to an inhibitor of carbonic anhydrase (CA) specifically designed to hyperthermally target tumours by application of a near infrared light, for example from a laser, or for treating other conditions in which the CA activity is involved.
  • NIR near infrared
  • CA carbonic anhydrase
  • NIR near infrared
  • NIR near infrared
  • the ligands are intended to exploit constitutive anomalies of a specific subpopulation of malignant cells, which may vary from lesion to lesion and provide poor specificity.
  • the variability underlies the complexity of cancer.
  • a deficit of specificity mirrors the lack of qualitative differences between normal and malignant cells, which often exhibit the same biochemical diversity but at different levels of expression. Examples include the overexpression of epidermal growth factor receptors, vascular endothelial growth factor receptors, folate receptors, etc., which reflects the higher metabolic activity of malignant cells. Although with a different density these species are common to both normal and malignant cells .
  • inhibitors of carbonic anhydrases have been reported for the management of a variety of disorders (glaucoma, edema, obesity, cancer, epilepsy, etc. (Supuran, Nature Rev. Drug. Discov. 7, 168-181 (2008)).
  • Carbonic anhydrases exist in several isoforms and are ubiquitous within eukaryotic cells.
  • Carbonic anhydrase 9 (CA9) is a transmembrane protein which is overexpressed by cells only under conditions of deficient oxygenation. Therefore an inhibitor of CA9 which may not penetrate plasmatic membranes would specifically bind hypoxic cells (Stiti, J. Am. Chem. Soc. 130, 16130-16131 (2008) ) .
  • Hypoxia occurs in a broad variety of solid tumors as a result of their abnormal growth and insufficient vascularization.
  • solid tumors feature a coexistence of necrotic, hypoxic and normoxic tissue.
  • cancer is maintained by a small population of cancer stem cells, which are able to indefinitely divide, exhibit resistance to chemotherapeutics and require a hypoxic environment.
  • WO2004048544 discloses aromatic sulphonamide and heterocyclic sulfonamide CA9 specific inhibitors.
  • US20070212305 discloses optical imaging techniques of lung cancer by means of a contrast agent linked with a ligand for an overexpressed target associated with lung cancer. Among the targets it is mentioned carbonic anhydrase.
  • US20090104179 discloses a luminescent material responsive to ionizing radiation and a photosensitive biologically active material .
  • US20110262500 discloses nanoparticles of gold, silver or platinum bound by means of a linker to a platinum compound for cancer treatment .
  • US20130004523 discloses paclitaxel covalently linked to a nanoparticle .
  • WO9725039 discloses the combination between carbonic anhydrase inhibitors and another agent for multimodal treatment of cancer.
  • WO2007046893 discloses a dock and lock method to assemble effector moieties for treating cancer.
  • effector moieties of interest are mentioned ligands and anticancer agents such as those for optical hyperthermia.
  • WO2012018383 discloses gold nanoparticles conjugates with thiloated hyperbranched dendrons with moieties capable to carry anticancer agents.
  • WO2006137009 and O2006137092 disclose CA9 inhibitors comprising a moiety of a fluorescent dye.
  • WO2008071421 discloses nitrate ester derivatives of carbonic anhydrase inhibitors.
  • CA9 inhibitors are disclosed in WO201109861, WO2012021963, WO2012070024 and WO201217565 .
  • US20120321563 discloses fluorescent imaging agents that bind to carbonic anhydrase, in particular CA9, used for identifying hypoxic cells in hypoxic tumors.
  • said agents comprise a far-red or near-infrared fluorophore linked to a carbonic anhydrase targeting agent.
  • the fluorophore is a chemical such as a cyanine dye, carbocyanine dye, indocyanine dye, or a polymethine fluorescent dye providing a fluorescent signal detectable by imaging techniques.
  • WO2012154885 discloses carbonic anhydrase targeting agents chemically linked to fluorophores in the far-red or near-infrared region or radiolabels, optionally by a linker, which can be used to image or deliver radiation to hypoxic tumors.
  • the compounds disclosed roughly spherical in shape, of empirical formulas [Au 7 2o ( C16H24O3 2 S3 ) 144 ] and [AU724 ( C17H21O3N3S4 ) 135 ] , have an average particle size of 3.3 nm which corresponds to ⁇ 720-724 Au atoms and a number of sulfonamide ligands attached to the NP as being 144 and 135 respectively.
  • [AU720 ( C16H24O3N2S 3 ) 144 ] shows excellent CA9 inhibitory properties and selectivity for the inhibition of the tumor-associated isoform over carbonic anhydrases 1 and 2, while [AU724 ( C17H21O3N3S4 ) 135] has been used as a control, since substituted sulfonamides do not act as CAIs.
  • the distinguishing feature of the present invention is the new covalent combination between an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light within the assembly of the present invention, wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 ran 2 .
  • the optical absorption cross section of the single unit of absorber of near infrared (NIR) light needs to exceed 100 ran 2 because the concentration of CA9 in hypoxic lesions is normally not higher than 10 12 - 10 13 molecules per ml (G.X. Zhou, J. Ireland, P. Rayman, J. Finke, M. Zhou, "Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials," Urology. 75, 257-61 (2010); M. Takacova, M. Bartosova, L. Skvarkova, M. Zatovicova, I. Vidlickova, L. Csaderova, M. Barathova, J.
  • Said combination shows a synergistic effect in improving efficacy and specificity of hyperthermia therapy.
  • the assembly of the present invention acts as anticancer agent in hyperthermia therapy.
  • the technical problem of the present invention is to provide anticancer agents different from those disclosed in the prior art having better properties when used in hyperthermia therapy.
  • the site-directed optical activation of the absorber of near infrared (NIR) light with an optical absorption cross section not lower than 100 nm 2 by local illumination of the tumour avoids the risk of damaging hypoxic benign lesions as well as those organs where the assembly may accumulate after systemic administration, such as liver and spleen.
  • NIR near infrared
  • said controlled optical activation also extends the effect of hyperthermia, primarily directed to the hypoxic core of the malignant lesion, to its adjacent normoxic periphery, thanks to heat dissipation.
  • the assembly between an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light with an optical absorption cross section not lower than 100 nm 2 enhances specificity and versatility .
  • NIR near infrared
  • the assembly of the present invention provides synergistic opportunities since both carbonic anhydrase targeting and optical hyperthermia profit from blood stagnation in solid tumours, which arouses hypoxia and jeopardizes the principal pathways of heat dissipation .
  • the technical problem has been solved by providing an assembly comprising an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light covalently bound by a linker wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 nm 2 , which is the object of the present invention.
  • NIR near infrared
  • Another object of the present invention is said assembly optionally further comprising at least one drug or oligonucleotide bound to the absorber of near infrared (NIR) light by a cross- linker able to dissociate at a temperature between 40-100°C.
  • NIR near infrared
  • Another object of the present invention is the use of said assembly as a contrast agent for photothermal treatments.
  • the assembly of the present invention may also be used as a contrast agent for photoacoustic imaging.
  • a further object of the present invention is the use of said assembly as a medicament, in particular as an anticancer agent in hyperthermia therapy of tumours or conditions in which the CA activity is involved.
  • step a) Contacting the NIR absorber covalently bound to the linker as obtained in step a) with the CAI and reacting to obtain the assemb1 .
  • step a) Contacting the CAI covalently bound to the linker as obtained in step a) with the NIR absorber and reacting to obtain the assembly.
  • Figure 1 shows a schematic representation of one object of this invention .
  • Figure 2 shows the targeting of gold particles with inhibitors of CA9. Images display HCT116 colon carcinoma cells expressing transmembrane carbonic anhydrases only under hypoxic conditions. The accumulation of gold particles is revealed by silver enhancement only in the presence of the carbonic anhydrase inhibitor and under hypoxia.
  • Figure 3 shows images of HCT116 colon carcinoma cells kept under hypoxic conditions and then treated for ten minutes with a NIR laser with a power density of 60 W cm -2 .
  • Panels display cells without particles (A) and incubated with PEGylated particles without (B) and with ligand (C) . After incubation, the growth medium was thoroughly replaced.
  • the synergistic effect of the inhibitor and optical excitation leads to cell death, which is identified by trypan blue staining in panel C. In the absence of ligand this optical fluence does not damage cells.
  • the power density required to reach the damage threshold both under hypoxia with particles without ligands and normoxia with particles with ligands falls between around 120 - 150 W cm -2 .
  • the main object of the present invention is an assembly comprising an absorber of near infrared (NIR) light and an inhibitor of carbonic anhydrase covalently bound by a linker wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 nm 2 .
  • NIR near infrared
  • optical hyperthermia requires an optical absorption coefficient (a) around 1 - 10 cm _1 in order to achieve contrast with endogenous tissue (see J. Mobley, T. Vo-Dinh, "Optical properties of tissue,” 2-60-70 in T. Vo-Dinh Ed., “Biomedical photonics handbook,” CRC Press, Boca Raton (2003)) and the concentration of CA9 in hypoxic tumors is normally below 10 12 - 10 13 molecules per ml (G.X. Zhou, J. Ireland, P. Rayman, J. Finke, M.
  • assembly means a combination of elements covalently linked to each other.
  • plasmonic particles means particles whose electron density can couple with electromagnetic radiation of wavelengths that are larger than the particle size due to the nature of the dielectric - metal interface between the medium and the particles.
  • absorber of near infrared (NIR) light means any compound capable to mediate photothermal conversion at NIR frequencies and having an optical absorption cross section not lower than 100 ran 2 .
  • nanoparticles containing near infrared dyes organic compounds with conjugated n systems containing polyene, polymethine and donor-acceptor near infrared chromophores, including indocyanine green, IR-780 iodide
  • near infrared dyes organic compounds with conjugated n systems containing polyene, polymethine and donor-acceptor near infrared chromophores, including indocyanine green, IR-780 iodide
  • organic nanoparticles such as single walled and multi walled carbon nanotubes, graphene, graphene oxide
  • metallic nanoparticles with near infrared plasmonic resonances such as gold nanorods, gold/silica nanoshells, gold nanocages, gold/gold sulfide nanoshells, hollow gold nanospheres
  • the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 10 2 to 10 5 ran 2 .
  • the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 10 3 to 10 4 ran 2 .
  • the absorber of near infrared (NIR) light is a plasmonic particle in a range of size between ⁇ 5 and 100 nm.
  • the absorber of near infrared (NIR) light is a gold nanorod .
  • the absorber of near infrared (NIR) light is impermeable to plasmatic membranes in order to inhibit its penetration inside the cellular cytoplasm and prevent its association with intracellular carbonic anhydrases isoforms.
  • NIR near infrared
  • inhibitor of carbonic anhydrase means any compound able to inhibit the activity of carbonic anhydrase by specific binding.
  • the inhibitor of carbonic anhydrase is an inhibitor of isoform carbonic anhydrase 9 (CA9) .
  • the inhibitor of carbonic anhydrase is the compound of general formula (I) :
  • n is an integer from 0 to 2 Q is absent or is 0 or N
  • X is N, 0, S or COOR
  • R is H, Me, Et, n-Pr, halogen
  • halogen means CI, F, I or Br
  • Ar is an aromatic or heteroaromatic ring optionally substituted with at least one substituent selected from the group consisting of: CI, F, I, Br, CN, OMe, Ph, PhO, PhCH 2 , N0 2 , Me 2 N, Me, Et0 2 C, Ac, EtO, iPr, MeS, Ci-C 20 alkyl, Ci-C 20 heteroalkyl, Ci-Cio alkyl-C 5 -C 6 aryl, Ci-C 20 heteroalkyl-C5-C6 aryl .
  • heteroalkyl refers to an alkyl group, in which one or more carbon atoms are replaced by an oxygen, nitrogen, phosphorus or sulphur
  • n 1 or 2
  • Ar is a phenyl or a phenyl substituted with a halogen in position 3.
  • the inhibitor of carbonic anhydrase is 4- (2- Aminoethyl) benzenesul fonamide .
  • linker means a simple bond between the absorber of near infrared (NIR) light and the inhibitor of carbonic anhydrase or a compound of the general formula (II) :
  • n is an integer from 1 to 4
  • X is NQ, 0 or S
  • R is NQH, OH, COOR'
  • Q is H, Ci-C 20 alkyl, Ci-C 20 heteroalkyl, Ci-Cio alkyl-C 5 -C 6 aryl, Ci- C 20 heteroalkyl-C5-C6 aryl.
  • X is 0, n is 2 or 3 and R is COOH.
  • X is 0, n is 2 and R is COOH and the linker is alpha mercapto omega carboxy polyethylene glycol with a M.W. of 5000.
  • the absorber of near infrared (NIR) light is coated with a biocompatible polymer, preferably a biocompatible polymer with a molecular weight between 1000 and 10000 g mol -1 .
  • biocompatible polymer means any polymer suitable for pharmaceutical use.
  • the biocompatible polymer is polyethylene glycol.
  • the assembly may optionally further comprise at least one drug or oligonucleotide bound to the absorber of near infrared (NIR) light by a cross-linker able to dissociate at a temperature between 40- 100°C.
  • NIR near infrared
  • the assembly releases the drug or oligonucleotide when subjected to near infrared light.
  • cross-linker is either of:
  • said two molecules comprise a couple of complementary oligonucleotide strands undergoing hybridization below 40°C and melting in a range of temperatures between 40 - 100°C.
  • the drug is selected from the group consisting of: alkylating agents, antimetabolites, anthracyclines , plant alkaloids, topoisomerase inhibitors, tyrosine kinase inhibitors, monoclonal antibodies and other antineoplastic agents.
  • the oligonucleotide is selected from the group consisting of: antisense, short interfering RNA, antigen, DNA decoy, RNA decoy, ribozyme and aptamer oligonucleotides.
  • Said assembly can be used as a contrast agent for photothermal treatments .
  • Said assembly can be used as a contrast agent for photoacoustic imaging .
  • a further object of the present invention is the use of said assembly as a medicament.
  • Another object of the present invention is the use of said assembly for use as an anticancer agent in hyperthermia therapy of tumours or conditions in which the CA activity is involved.
  • tumours are: carcinomas, sarcomas, lymphomas, leukemias, germ cell tumours, blastomas, melanomas, brain tumours and nervous system tumours.
  • the conditions in which the CA activity is involved are for example cancer, tumour, glaucoma, obesity, osteoporosis, Alzheimer, epilepsy.
  • the assembly of the present invention may be prepared by a process comprising the following steps:
  • step a) Contacting the NIR absorber covalently bound to the linker as obtained in step a) with the CAI and reacting to obtain the assemb1y .
  • step a) Contacting the CAI covalently bound to the linker as obtained in step a) with the NIR absorber and reacting to obtain the assembly.
  • the CAI displays an amino moiety and the cross linker contains mercapto and carboxyl terminations which bind the gold particle and amino moiety from the CAI by amidation.
  • the above gold nanorods are modified with a mixture of ⁇ 90 % alpha mercapto omega methoxy and 10 % alpha mercapto omega carboxy polyethylene glycol (MW 5000 g mol "1 ) .
  • Gold nanorods with effective radii of ⁇ 10 nm and an aspect ratio of ⁇ 4 may be conjugated with 4- (2-aminoethyl) enzenesulfonamide by the use of an alpha mercapto omega carboxy polyethylene glycol cross linker, binding the gold particle through a gold sulphur bridge and the amino termination from 4- (2- aminoethyl ) benzenesul fonamide upon amidation.
  • Gold nanorods were synthesised at 25°C in the dark.
  • a seed suspension was prepared by rapid, injection of ice cold 10 mM aqueous sodium borohydride into a solution containing 100 mM cetrimonium bromide and 240 uM chloroauric acid until a final concentration of 560 ⁇ sodium borohydride. This suspension was left under vigorous agitation for 10 min and then at rest for another 2 hours before rapid addition into a growth solution.
  • the above assembly proves adequate to minimize the unspecific uptake of the particles by normoxic cells and enables an adequate load of the inhibitor by amidation.
  • the prepared assemblies prove stable in physiological buffers and biological fluids and display irrelevant cytotoxicity up to 400 ⁇ gold. Their cellular internalization may only occur via endocytotic pathways, i.e. these particles may not come into contact with intracellular carbonic anhydrases isoforms. Conversely their cellular uptake correlates with the expression of CA9, which may be modulated with oxygenation as it is documented in the scientific literature.
  • FIG. 2 The results of targeting with inhibitors of CA9 are presented in figure 2 wherein the images display HCT116 colon carcinoma cells expressing transmembrane carbonic anhydrases only under hypoxic conditions.
  • the accumulation of gold particles is revealed by silver enhancement only in the presence of the carbonic anhydrase inhibitor and under hypoxia.
  • the accumulation of these particles induces a substantial sensitization and decrease of the thresholds for cellular damage upon irradiation with NIR light under CW illumination. This effect already becomes visible in a cellular monolayer despite its inefficient heat confinement.
  • Figure 3 reports images showing HCT116 colon carcinoma cells maintained under hypoxic conditions and then treated for ten minutes with a NIR laser diode with a power density of 60 W cm -2 .
  • Panels display cells without particles (A) and incubated with PEGylated particles without (B) and with ligand (C) . After incubation, the cellular medium was thoroughly replaced. The synergistic effect of the inhibitor and optical excitation leads to cell death, which is identified by trypan blue staining in panel C. In the absence of ligand this optical fluence does not damage cells.
  • the power density required to reach the damage threshold both under hypoxia with particles without ligands and normoxia with particles with ligands falls between around 120 - 150 W cirf 2 .

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Abstract

An assembly comprising an absorber of near infrared (NIR) light having an optical absorption cross section not lower than 100 nm2 covalently linked to an inhibitor of carbonic anhydrase (CA), process for its preparation and its use to hyperthermally target tumours or for treating other conditions in which the CA activity is involved is disclosed.

Description

ASSEMBLY COMPRISING AN ABSORBER OF NEAR INFRARED (NIR) LIGHT COVALENTLY LINKED TO AN INHIBITOR OF CARBONIC ANHYDRASE
*****
Background of the invention
The present invention refers to the pharmaceutical field and in particular to an assembly comprising an absorber of near infrared (NIR) light covalently linked to an inhibitor of carbonic anhydrase (CA) specifically designed to hyperthermally target tumours by application of a near infrared light, for example from a laser, or for treating other conditions in which the CA activity is involved.
Prior Art
It is known in the art the use of absorbers of near infrared (NIR) light for photo-acoustic imaging and optical hyperthermia of cancer .
Furthermore, combinations of absorbers of near infrared (NIR) light with ligands are known in the art. The ligands are intended to exploit constitutive anomalies of a specific subpopulation of malignant cells, which may vary from lesion to lesion and provide poor specificity. The variability underlies the complexity of cancer. A deficit of specificity mirrors the lack of qualitative differences between normal and malignant cells, which often exhibit the same biochemical diversity but at different levels of expression. Examples include the overexpression of epidermal growth factor receptors, vascular endothelial growth factor receptors, folate receptors, etc., which reflects the higher metabolic activity of malignant cells. Although with a different density these species are common to both normal and malignant cells .
Wang M. and Thanou M., 2010, "Targeting nanoparticles to cancer", Pharmacological Research 62, 90-99 reviews nanotechnology applications in medicine, wherein devices with dimensions on the nanometer length scale can carry detection signals and/or therapeutic cargos and are designed to reach their target and specifically release their load, thus enhancing the drug's tissue bioavailability. They comprise liposomes, solid lipid nanoparticles (SLN) , dendrimers, iron oxide nanoparticles used for imaging purposes, noble metal nanoparticles such as gold, noble metal nanoshells consisting of a dielectric bead surrounded by a thin metal shell, such as gold. For example, NIR laser treatment of the bulk tissue selectively heats and destroys the nanoshell- laden tumor tissue, while leaving surrounding tissue intact, or nanorods with the appropriate PEG stealth layer are being developed as an improved means of optical hyperthermia.
Huang X. et al . , 2010, "A Reexamination of Active and Passive Tumor Targeting by Using Rod-Shaped Gold Nanocrystals and Covalently Conjugated Peptide Ligands", 2010, ACSNANO 4, 5887- 5896, discloses colloidal gold nanorods stabilized with polyethylene glycols (PEGs) conjugated to three different ligands: a single-chain variable fragment (ScFv) peptide that recognizes the epidermal growth factor receptor (EGFR) ; an amino terminal fragment (ATF) peptide that recognizes the urokinase plasminogen activator receptor (uPAR) ; a cyclic RGD peptide that recognizes the ay 3 integrin receptor. Quantitative tumour uptake studies show that the use of active targeting ligands only marginally improves the total tumor uptake in comparison with control particles resting on the enhanced permeability and retention (EPR) pathway only. However, the nature of targeting ligands can affect the nanoparticle distribution in the tumor cells and the tumor microenvironments , suggesting that for photothermal cancer therapy, the preferred route of gold nanorods is intratumoral administration .
The use of inhibitors of carbonic anhydrases as therapeutic agents has been reported for the management of a variety of disorders (glaucoma, edema, obesity, cancer, epilepsy, etc. (Supuran, Nature Rev. Drug. Discov. 7, 168-181 (2008)).
Carbonic anhydrases exist in several isoforms and are ubiquitous within eukaryotic cells. However, Carbonic anhydrase 9 (CA9) is a transmembrane protein which is overexpressed by cells only under conditions of deficient oxygenation. Therefore an inhibitor of CA9 which may not penetrate plasmatic membranes would specifically bind hypoxic cells (Stiti, J. Am. Chem. Soc. 130, 16130-16131 (2008) ) . Hypoxia occurs in a broad variety of solid tumors as a result of their abnormal growth and insufficient vascularization. As a rule solid tumors feature a coexistence of necrotic, hypoxic and normoxic tissue. Furthermore, it is widely recognized that cancer is maintained by a small population of cancer stem cells, which are able to indefinitely divide, exhibit resistance to chemotherapeutics and require a hypoxic environment.
WO2004048544 discloses aromatic sulphonamide and heterocyclic sulfonamide CA9 specific inhibitors. US20070212305 discloses optical imaging techniques of lung cancer by means of a contrast agent linked with a ligand for an overexpressed target associated with lung cancer. Among the targets it is mentioned carbonic anhydrase.
US20090104179 discloses a luminescent material responsive to ionizing radiation and a photosensitive biologically active material .
US20110262500 discloses nanoparticles of gold, silver or platinum bound by means of a linker to a platinum compound for cancer treatment .
US20130004523 discloses paclitaxel covalently linked to a nanoparticle .
WO9725039 discloses the combination between carbonic anhydrase inhibitors and another agent for multimodal treatment of cancer.
WO2007046893 discloses a dock and lock method to assemble effector moieties for treating cancer. Among the effector moieties of interest are mentioned ligands and anticancer agents such as those for optical hyperthermia.
WO2012018383 discloses gold nanoparticles conjugates with thiloated hyperbranched dendrons with moieties capable to carry anticancer agents.
WO2006137009 and O2006137092 disclose CA9 inhibitors comprising a moiety of a fluorescent dye.
WO2008071421 discloses nitrate ester derivatives of carbonic anhydrase inhibitors.
Further classes of CA9 inhibitors are disclosed in WO201109861, WO2012021963, WO2012070024 and WO201217565 .
US20120321563 discloses fluorescent imaging agents that bind to carbonic anhydrase, in particular CA9, used for identifying hypoxic cells in hypoxic tumors. In particular said agents comprise a far-red or near-infrared fluorophore linked to a carbonic anhydrase targeting agent. The fluorophore is a chemical such as a cyanine dye, carbocyanine dye, indocyanine dye, or a polymethine fluorescent dye providing a fluorescent signal detectable by imaging techniques.
WO2012154885 discloses carbonic anhydrase targeting agents chemically linked to fluorophores in the far-red or near-infrared region or radiolabels, optionally by a linker, which can be used to image or deliver radiation to hypoxic tumors.
Ratto F. et al., 2011, "Gold nanorods as new nanochromophores for photothermal therapies", Journal of Biophotonics 4, 64-73, disclose the use of colloidal gold nanorods excited by diode laser radiation at 810 nm to mediate functional photothermal effects, in particular laser welding mediated by aqueous colloids of gold nanorods or gold nanorods into biopolymeric solders. The authors also speculate the possible use of gold nanorods conjugated with antibodies, aptamers, natural factors, peptides, etc. in diagnosis, imaging and therapy of cancer.
Stiti M. et al . , 2008, "Carbonic anhydrase inhibitor coated gold nanoparticles selectively inhibit the tumor-associated isoform IX over the cytosolic isozymes I and II", J. Am. Chem. Soc. 130, 16130-16131 disclose gold nanoparticles coated with sulphonamide carbonic anhydrase inhibitors which can be used for targeting tumors overexpressing CA9. Said gold nanoparticles are not near infrared absorbers.
The compounds disclosed, roughly spherical in shape, of empirical formulas [Au72o ( C16H24O3 2 S3 ) 144 ] and [AU724 ( C17H21O3N3S4 ) 135 ] , have an average particle size of 3.3 nm which corresponds to ~ 720-724 Au atoms and a number of sulfonamide ligands attached to the NP as being 144 and 135 respectively.
[AU720 ( C16H24O3N2S 3 ) 144 ] shows excellent CA9 inhibitory properties and selectivity for the inhibition of the tumor-associated isoform over carbonic anhydrases 1 and 2, while [AU724 ( C17H21O3N3S4 ) 135] has been used as a control, since substituted sulfonamides do not act as CAIs.
Groves et al . , 2011, Bioorganic & Medicinal Chemistry Letters, 22(1), 653:657 disclose human carbonic anhydrase (hCA) IX inhibitors conjugated to fluorescent dyes with the aim of imaging hypoxia-induced hCA IX expression in tumour cells.
Tafreshi et al . , 2012, Clinical Cancer Research, 18(1), 207:219 disclose carbonic anhydrase IX-specific monoclonal antibodies conjugated to a near-infrared fluorescent dye as molecular imaging probes .
Maamar Stiti et al., 2008, Journal of the American Chemical Society, 130(48), 16130:16131 disclose gold nanoparticles coated with sulfonamide carbonic anhydrase inhibitors.
Ratto et al., 2009, Journal of Nanoparticle Research, 12(6), 2029:2036 report the control of the overgrowth of gold nanorods by modulating their size and shape.
The distinguishing feature of the present invention is the new covalent combination between an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light within the assembly of the present invention, wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 ran2.
The optical absorption cross section of the single unit of absorber of near infrared (NIR) light needs to exceed 100 ran2 because the concentration of CA9 in hypoxic lesions is normally not higher than 1012 - 1013 molecules per ml (G.X. Zhou, J. Ireland, P. Rayman, J. Finke, M. Zhou, "Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials," Urology. 75, 257-61 (2010); M. Takacova, M. Bartosova, L. Skvarkova, M. Zatovicova, I. Vidlickova, L. Csaderova, M. Barathova, J. Breza, P. Bujdak, J. Pastorek, J. Breza, S. Pastorekova, "Carbonic anhydrase IX is a clinically significant tissue and serum biomarker associated with renal cell carcinoma," Oncol Lett. 5, 191-7 (2013)) and the optical absorption coefficient needed for optical hyperthermia is in the order of 1 - 10 cnT1 (i.e. higher than that typical of endogenous tissue, see J. Mobley, T. Vo-Dinh, "Optical properties of tissue," 2-60-70 in T. Vo-Dinh Ed., "Biomedical photonics handbook," CRC Press, Boca Raton (2003)).
Said combination shows a synergistic effect in improving efficacy and specificity of hyperthermia therapy.
On optical stimulation, the assembly of the present invention acts as anticancer agent in hyperthermia therapy.
The technical problem of the present invention is to provide anticancer agents different from those disclosed in the prior art having better properties when used in hyperthermia therapy.
The combination between an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light with an optical absorption cross section not lower than 100 ran2, which is inert without optical activation, allows to specifically target malignant lesions .
Moreover, the site-directed optical activation of the absorber of near infrared (NIR) light with an optical absorption cross section not lower than 100 nm2 by local illumination of the tumour avoids the risk of damaging hypoxic benign lesions as well as those organs where the assembly may accumulate after systemic administration, such as liver and spleen.
Moreover, said controlled optical activation also extends the effect of hyperthermia, primarily directed to the hypoxic core of the malignant lesion, to its adjacent normoxic periphery, thanks to heat dissipation.
The assembly between an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light with an optical absorption cross section not lower than 100 nm2 enhances specificity and versatility .
Specificity arises from the high contrast in terms of expression of transmembrane carbonic anhydrase between normoxic and hypoxic tissue, while versatility originates from the ubiquity of carbonic anhydrase 9 expression under hypoxic conditions.
The assembly of the present invention provides synergistic opportunities since both carbonic anhydrase targeting and optical hyperthermia profit from blood stagnation in solid tumours, which arouses hypoxia and jeopardizes the principal pathways of heat dissipation .
Object of the Invention
As already discussed, the technical problem has been solved by providing an assembly comprising an inhibitor of carbonic anhydrase and an absorber of near infrared (NIR) light covalently bound by a linker wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 nm2, which is the object of the present invention.
Another object of the present invention is said assembly optionally further comprising at least one drug or oligonucleotide bound to the absorber of near infrared (NIR) light by a cross- linker able to dissociate at a temperature between 40-100°C.
Another object of the present invention is the use of said assembly as a contrast agent for photothermal treatments.
The assembly of the present invention may also be used as a contrast agent for photoacoustic imaging.
A further object of the present invention is the use of said assembly as a medicament, in particular as an anticancer agent in hyperthermia therapy of tumours or conditions in which the CA activity is involved.
It is an object of the present invention a process for the preparation of said assembly comprising the following steps:
) Contacting the NIR absorber with the linker and reacting to obtain a covalent bond between the NIR absorber and the linker;
) Contacting the NIR absorber covalently bound to the linker as obtained in step a) with the CAI and reacting to obtain the assemb1 .
Or alternatively:
) Contacting the CAI with the linker and reacting to obtain a covalent bond between the CAI and the linker;
) Contacting the CAI covalently bound to the linker as obtained in step a) with the NIR absorber and reacting to obtain the assembly.
Further features of the present invention will be clear from the following detailed description with references to experimental examples and attached figures.
Brief description of the figures
Figure 1 shows a schematic representation of one object of this invention .
Figure 2 shows the targeting of gold particles with inhibitors of CA9. Images display HCT116 colon carcinoma cells expressing transmembrane carbonic anhydrases only under hypoxic conditions. The accumulation of gold particles is revealed by silver enhancement only in the presence of the carbonic anhydrase inhibitor and under hypoxia.
Figure 3 shows images of HCT116 colon carcinoma cells kept under hypoxic conditions and then treated for ten minutes with a NIR laser with a power density of 60 W cm-2. Panels display cells without particles (A) and incubated with PEGylated particles without (B) and with ligand (C) . After incubation, the growth medium was thoroughly replaced. The synergistic effect of the inhibitor and optical excitation leads to cell death, which is identified by trypan blue staining in panel C. In the absence of ligand this optical fluence does not damage cells. The power density required to reach the damage threshold both under hypoxia with particles without ligands and normoxia with particles with ligands falls between around 120 - 150 W cm-2. Detailed description of the invention
The main object of the present invention is an assembly comprising an absorber of near infrared (NIR) light and an inhibitor of carbonic anhydrase covalently bound by a linker wherein the absorber of near infrared (NIR) light has an optical absorption cross section not lower than 100 nm2.
The above absorber of near infrared (NIR) light is not a single fluorescent molecule since the value of optical absorption cross section for single fluorescent molecules is in the order of 0.01 nm2 (e.g. about 0.02 nm2 for indocyanine green in water, which is among the most efficient near infrared dyes) .
The above values are calculated considering the following parameters: optical hyperthermia requires an optical absorption coefficient (a) around 1 - 10 cm _1 in order to achieve contrast with endogenous tissue (see J. Mobley, T. Vo-Dinh, "Optical properties of tissue," 2-60-70 in T. Vo-Dinh Ed., "Biomedical photonics handbook," CRC Press, Boca Raton (2003)) and the concentration of CA9 in hypoxic tumors is normally below 1012 - 1013 molecules per ml (G.X. Zhou, J. Ireland, P. Rayman, J. Finke, M. Zhou, "Quantification of carbonic anhydrase IX expression in serum and tissue of renal cell carcinoma patients using enzyme-linked immunosorbent assay: prognostic and diagnostic potentials, " Urology. 75, 257-61 (2010); M. Takacova, M. Bartosova, L. Skvarkova, M. Zatovicova, I. Vidlickova, L. Csaderova, M. Barathova, J. Breza, P. Bujdak, J. Pastorek, J. Breza, S. Pastorekova, "Carbonic anhydrase IX is a clinically significant tissue and serum biomarker associated with renal cell carcinoma, " Oncol Lett. 5, 191-7 (2013)), which represents an upper limit for the density (d) of absorbers of near infrared (NIR) light that may be accumulated in hypoxic tumors by the use of this molecular target. A lower limit for the optical absorption cross section (σ) of the single unit of absorber of near infrared (NIR) light is given by the formula σ = a / d.
Within the meaning of the present invention assembly means a combination of elements covalently linked to each other.
Within the meaning of the present invention plasmonic particles means particles whose electron density can couple with electromagnetic radiation of wavelengths that are larger than the particle size due to the nature of the dielectric - metal interface between the medium and the particles. Within the meaning of the present invention absorber of near infrared (NIR) light means any compound capable to mediate photothermal conversion at NIR frequencies and having an optical absorption cross section not lower than 100 ran2. Examples include nanoparticles containing near infrared dyes (organic compounds with conjugated n systems containing polyene, polymethine and donor-acceptor near infrared chromophores, including indocyanine green, IR-780 iodide) such as solid lipid, liposomal, micellar, porous silica, polymeric, viral carriers; phospholipid porphyrin conjugates; organic nanoparticles such as single walled and multi walled carbon nanotubes, graphene, graphene oxide; metallic nanoparticles with near infrared plasmonic resonances such as gold nanorods, gold/silica nanoshells, gold nanocages, gold/gold sulfide nanoshells, hollow gold nanospheres; upconversion nanoparticles containing lanthanide moieties, and combinations thereof .
Preferably the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 102 to 105 ran2.
More preferably the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 103 to 104 ran2.
Preferably the absorber of near infrared (NIR) light is a plasmonic particle in a range of size between ~ 5 and 100 nm.
Preferably the absorber of near infrared (NIR) light is a gold nanorod .
Preferably the absorber of near infrared (NIR) light is impermeable to plasmatic membranes in order to inhibit its penetration inside the cellular cytoplasm and prevent its association with intracellular carbonic anhydrases isoforms.
Within the meaning of the present invention inhibitor of carbonic anhydrase means any compound able to inhibit the activity of carbonic anhydrase by specific binding.
Preferably the inhibitor of carbonic anhydrase is an inhibitor of isoform carbonic anhydrase 9 (CA9) .
Preferably the inhibitor of carbonic anhydrase is the compound of general formula (I) :
X- (CH2) n-Ar- [Q] -SO2NH2 (I)
wherein
n is an integer from 0 to 2 Q is absent or is 0 or N
X is N, 0, S or COOR
R is H, Me, Et, n-Pr, halogen
Wherein halogen means CI, F, I or Br
Ar is an aromatic or heteroaromatic ring optionally substituted with at least one substituent selected from the group consisting of: CI, F, I, Br, CN, OMe, Ph, PhO, PhCH2, N02, Me2N, Me, Et02C, Ac, EtO, iPr, MeS, Ci-C20 alkyl, Ci-C20 heteroalkyl, Ci-Cio alkyl-C5-C6 aryl, Ci-C20 heteroalkyl-C5-C6 aryl .
The term heteroalkyl refers to an alkyl group, in which one or more carbon atoms are replaced by an oxygen, nitrogen, phosphorus or sulphur
Preferably n is 1 or 2, Ar is a phenyl or a phenyl substituted with a halogen in position 3.
Preferably the inhibitor of carbonic anhydrase is 4- (2- Aminoethyl) benzenesul fonamide .
Within the meaning of the present invention linker means a simple bond between the absorber of near infrared (NIR) light and the inhibitor of carbonic anhydrase or a compound of the general formula (II) :
-HS-[ (CH2)n-X- (CH2)n]-R- (ID
wherein
n is an integer from 1 to 4
X is NQ, 0 or S
R is NQH, OH, COOR'
R'is H, Me, Et,n-Pr, halogen
Q is H, Ci-C20 alkyl, Ci-C20 heteroalkyl, Ci-Cio alkyl-C5-C6 aryl, Ci- C20 heteroalkyl-C5-C6 aryl.
Preferably X is 0, n is 2 or 3 and R is COOH.
More preferably X is 0, n is 2 and R is COOH and the linker is alpha mercapto omega carboxy polyethylene glycol with a M.W. of 5000.
Optionally the absorber of near infrared (NIR) light is coated with a biocompatible polymer, preferably a biocompatible polymer with a molecular weight between 1000 and 10000 g mol-1. Within the meaning of the present invention biocompatible polymer means any polymer suitable for pharmaceutical use.
Preferably the biocompatible polymer is polyethylene glycol.
The assembly may optionally further comprise at least one drug or oligonucleotide bound to the absorber of near infrared (NIR) light by a cross-linker able to dissociate at a temperature between 40- 100°C.
In this case the assembly releases the drug or oligonucleotide when subjected to near infrared light.
Preferably the cross-linker is either of:
a molecule covalently bound to the absorber of NIR light and the drug or oligonucleotide
or
two molecules non-covalently bound to each other, where the one is covalently bound to the absorber of NIR light and the other to the drug or oligonucleotide.
More preferably said two molecules comprise a couple of complementary oligonucleotide strands undergoing hybridization below 40°C and melting in a range of temperatures between 40 - 100°C.
Preferably the drug is selected from the group consisting of: alkylating agents, antimetabolites, anthracyclines , plant alkaloids, topoisomerase inhibitors, tyrosine kinase inhibitors, monoclonal antibodies and other antineoplastic agents.
Preferably the oligonucleotide is selected from the group consisting of: antisense, short interfering RNA, antigen, DNA decoy, RNA decoy, ribozyme and aptamer oligonucleotides.
Said assembly can be used as a contrast agent for photothermal treatments .
Said assembly can be used as a contrast agent for photoacoustic imaging .
A further object of the present invention is the use of said assembly as a medicament.
Another object of the present invention is the use of said assembly for use as an anticancer agent in hyperthermia therapy of tumours or conditions in which the CA activity is involved.
Within the meaning of the present invention tumours are: carcinomas, sarcomas, lymphomas, leukemias, germ cell tumours, blastomas, melanomas, brain tumours and nervous system tumours.
Within the meaning of the present invention, the conditions in which the CA activity is involved are for example cancer, tumour, glaucoma, obesity, osteoporosis, Alzheimer, epilepsy.
The assembly of the present invention may be prepared by a process comprising the following steps:
) Contacting the NIR absorber with the linker and reacting to obtain a covalent bond between the NIR absorber and the linker;
) Contacting the NIR absorber covalently bound to the linker as obtained in step a) with the CAI and reacting to obtain the assemb1y .
Or alternatively:
) Contacting the CAI with the linker and reacting to obtain a covalent bond between the CAI and the linker;
) Contacting the CAI covalently bound to the linker as obtained in step a) with the NIR absorber and reacting to obtain the assembly.
In a preferred embodiment wherein the near infrared absorber is a gold particle, the CAI displays an amino moiety and the cross linker contains mercapto and carboxyl terminations which bind the gold particle and amino moiety from the CAI by amidation.
In a preferred embodiment of the present invention the assembly comprises gold nanorods with effective radii (r = (3*V/ (4*n) ) (1/3) , with V particle volume) of ~ 10 nm and an aspect ratio (AR = 1/d, with 1 and d particle length and diameter respectively) of ~ 4, which has an estimated optical absorption cross section of ~ 4000 nm2, conjugated with 4- (2- aminoethyl ) benzenesul fonamide by means of an alpha mercapto omega carboxy polyethylene glycol cross linker binding the gold particle through a gold sulphur bridge and the amino termination from 4- (2- aminoethyl ) benzenesul fonamide upon amidation.
In a further preferred embodiment the above gold nanorods are modified with a mixture of ~ 90 % alpha mercapto omega methoxy and 10 % alpha mercapto omega carboxy polyethylene glycol (MW 5000 g mol"1) .
Examples
Gold nanorods with effective radii of ~ 10 nm and an aspect ratio of ~ 4 may be conjugated with 4- (2-aminoethyl) enzenesulfonamide by the use of an alpha mercapto omega carboxy polyethylene glycol cross linker, binding the gold particle through a gold sulphur bridge and the amino termination from 4- (2- aminoethyl ) benzenesul fonamide upon amidation.
Gold nanorods were synthesised at 25°C in the dark. A seed suspension was prepared by rapid, injection of ice cold 10 mM aqueous sodium borohydride into a solution containing 100 mM cetrimonium bromide and 240 uM chloroauric acid until a final concentration of 560 μΜ sodium borohydride. This suspension was left under vigorous agitation for 10 min and then at rest for another 2 hours before rapid addition into a growth solution. Meanwhile 500 ml growth solution were prepared with 100 mM cetrimonium bromide, 470 μΜ chloroauric acid, 92 μΜ silver nitrate and 520 μΜ ascorbic acid, which was dosed with a burette in order to keep a precise ratio between gold ions and their reducing agents. This growth solution was injected with 1 ml seed suspension and left under mild agitation for 30 min and then at rest for 24 hours. Finally 100 mM aqueous ascorbic a.cid was added until a total content of 710 uM ascorbic acid, which served to complete the metal reduction, as it is described elsewhere (Ratto, J. Na.nopa.rt. Res. 12, 2029-2036 (2010)).
After two cycles of centrifugation and decantation (dead volume ratio ~ 1 / 200), these particles were transferred at a rate of 1.9 mM gold into an acetate buffer at pH 5 containing 500 uM cetrimonium bromide and 50 μ.Μ of a mixture of ~ 90 % alpha mercapto omega methoxy and 10 % alpha mercapto omega carboxy polyethylene glycol (MW 5000 g rnol"1) . This suspension was left to react at 37°C for two hours before centrif gation, decantation and resuspension in 1% (v/v) aqueous polysorbate 20, which was left at rest for 2 hours at 37°C in an attempt to extract possible contaminants .
After purification by two cycles of centrifugation and decantation, these particles were transferred at a rate of 1.9 mM gold into a MES buffer at pH 6 containing 130 mM NaCl and 0.005% (v/v) polysorbate 20. In a typical modification, 1.5 ml of this suspension were activated 'with 1.5 ml 16 M l-ethyl-3- (3- dimethylaminopropyl ) carbodiimide and 4 mM N-hydroxysuccinimide, left at rest for 15 min, added with 3 ml 200 uM 4- (2- aminoethyl ) benzenesulfonamide and then kept at rest for another 2 hours. Chemicals were dissolved in a MES buffer at pH 6 containing 130 mM NaCl and 0.005% (v/v) polysorbate 20 and maintained at 37 °C throughout the modification. Finally these particles were transferred at a rate of 4.0 mM gold into sterile PBS after purification by four cycles of centrifligation and decantation .
The above assembly proves adequate to minimize the unspecific uptake of the particles by normoxic cells and enables an adequate load of the inhibitor by amidation. The prepared assemblies prove stable in physiological buffers and biological fluids and display irrelevant cytotoxicity up to 400 μΜ gold. Their cellular internalization may only occur via endocytotic pathways, i.e. these particles may not come into contact with intracellular carbonic anhydrases isoforms. Conversely their cellular uptake correlates with the expression of CA9, which may be modulated with oxygenation as it is documented in the scientific literature.
The results of targeting with inhibitors of CA9 are presented in figure 2 wherein the images display HCT116 colon carcinoma cells expressing transmembrane carbonic anhydrases only under hypoxic conditions. The accumulation of gold particles is revealed by silver enhancement only in the presence of the carbonic anhydrase inhibitor and under hypoxia. In turn the accumulation of these particles induces a substantial sensitization and decrease of the thresholds for cellular damage upon irradiation with NIR light under CW illumination. This effect already becomes visible in a cellular monolayer despite its inefficient heat confinement.
Figure 3 reports images showing HCT116 colon carcinoma cells maintained under hypoxic conditions and then treated for ten minutes with a NIR laser diode with a power density of 60 W cm-2. Panels display cells without particles (A) and incubated with PEGylated particles without (B) and with ligand (C) . After incubation, the cellular medium was thoroughly replaced. The synergistic effect of the inhibitor and optical excitation leads to cell death, which is identified by trypan blue staining in panel C. In the absence of ligand this optical fluence does not damage cells. The power density required to reach the damage threshold both under hypoxia with particles without ligands and normoxia with particles with ligands falls between around 120 - 150 W cirf2.

Claims

1) Assembly comprising an absorber of near infrared (NIR) light having an optical absorption cross section not lower than 100 nm2 and an inhibitor of carbonic anhydrase covalently bound by a linker .
2) Assembly according to claim 1 wherein the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 102 to 105 nm2.
3) Assembly according to claim 1 wherein the absorber of near infrared (NIR) light has an optical absorption cross section ranging from 103 to 104 nm2.
4) Assembly according to anyone of claims 1-3 wherein the absorber of near infrared (NIR) light is selected from the group consisting of: nanoparticles containing near infrared dyes (organic compounds with conjugated n systems containing polyene, polymethine or donor-acceptor near infrared chromophores, including indocyanine green, IR-780 iodide) within solid lipid, liposomal, micellar, porous silica, polymeric or viral carriers; phospholipid porphyrin conjugates; organic nanoparticles comprising single walled carbon nanotubes, multi walled carbon nanotubes, graphene, graphene oxide; metallic nanoparticles with near infrared plasmonic resonances comprising gold nanorods, gold/silica nanoshells, gold nanocages, gold/gold sulfide nanoshells, hollow gold nanospheres; up-conversion nanoparticles containing lanthanide moieties, and combinations thereof.
5) Assembly according to claim 4 wherein the absorber of near infrared (NIR) light is a plasmonic particle.
6) Assembly according to claim 5 wherein the plasmonic particle has a size between ~ 5 and 100 nm.
7) Assembly according to claim 5 wherein the plasmonic particle is a gold nanorod.
8) Assembly according to anyone of claims 1-3 wherein the inhibitor of carbonic anhydrase is an inhibitor of carbonic anhydrase 9 (CA9) .
9) Assembly according to anyone of claims 1-3 wherein the inhibitor of carbonic anhydrase is the compound of general formula (I) :
X- (CH2) n-Ar- [Q] -SO2NH2
wherein n is an integer from 0 to 2
Q is absent or is 0 or N
X is N, 0, S or COOR
R is H, Me, Et, n-Pr, halogen
Ar is an aromatic or heteroaromatic ring optionally substituted with at least one substituent selected from the group consisting of: CI, F, I, Br, CN, OMe, Ph, PhO, PhCH2, N02, Me2N, Me, Et02C, Ac, EtO, iPr, MeS, Ci-C20 alkyl, Ci-C20 heteroalkyl, Ci-Cio alkyl-C5-C6 aryl, Ci-C20 heteroalkyl-C5-C6 aryl .
10) Assembly according to claim 9 wherein in general formula (I) n is 1 or 2, Ar is a phenyl or a phenyl substituted with a halogen in position 3.
1 1) Assembly according to claim 9 wherein the inhibitor of carbonic anhydrase is 4- ( 2-Aminoethyl ) benzenesul fonamide .
12) Assembly according to anyone of claims 1-3 wherein the linker is a simple bond between the absorber of near infrared (NIR) light and the inhibitor of carbonic anhydrase or a compound of general formula (II) :
-HS-[ (CH2)n-X- ( CH2)n]-R- wherein
n is an integer from 1 to 4
X is NQ, 0 or S
R is NQH, OH, COOR'
R'is H, Me, Et,n-Pr, halogen
Q is H, Ci-C20 alkyl, Ci-C20 heteroalkyl, Ci-Cio alkyl-Cs-C6 aryl, Ci-C20 heteroalkyl-C5-C6 aryl
13) Assembly according to claim 12 wherein in general formula (II) X is 0, n is 2 or 3 and R is COOH.
14) Assembly according to claim 13 wherein in general formula (II) X is 0, n is 2 and R is COOH.
15) Assembly according to claim 14 wherein the linker is alpha mercapto omega carboxy polyethylene glycol with a M.W. of 5000.
16) Assembly according to anyone of claims 1-3 wherein the absorber of near infrared (NIR) light is coated with a biocompatible polymer . 17) Assemb1y according to claim 16 wherein the biocompatible polymer has a molecular weight between 1000 and 10000 g mol"1.
18) Assembly according to claim 17 wherein the biocompatible polymer is polyethylene glycol.
19) Assembly according to anyone of claims 1-3 wherein gold nanorods with effective radii of ~ 10 nm and an aspect ratio of ~ 4 are linked to 4- (2-aminoethyl) benzenesul fonamide by means of an alpha mercapto omega carboxy polyethylene glycol cross linker.
20) ssembly according to anyone of claims 1-3 optionally further comprising at least one drug or oligonucleotide bound to the absorber of near infrared (NIR) light by a cross-linker able to dissociate at a temperature between 40-100°C.
21) Assembly according to anyone of claims 1-20 for use as contrast agent for photothermal treatments .
22) Assembly according to anyone of claims 1-20 for use as a contrast agent for photoacoustic imaging.
23) Assembly according to anyone of claims 1-20 for use as a medicament .
24) Assembly according to anyone of claims 1-20 for use as anticancer agent in hyperthermia therapy of tumours or conditions in which the CA activity is involved.
25) Assembly according to anyone of claims 1-20 for use as carrier for photothermal release of drugs and oligonucleotides.
26) Use according to claim 24 wherein the condition in which the CA activity is involved is selected from the group consisting of: cancer, tumour, glaucoma, obesity, osteoporosis, Alzheimer, epilepsy .
27) Process for the preparation of the assembly of anyone of claims 1- 3 comprising the following steps:
a) Contacting the NIR absorber with the linker and reacting to obtain a covalent bond between the NIR absorber and the linker;
b) Contacting the NIR absorber covalently bound to the linker as obtained in step a) with the CAI and reacting to obtain the assemb1y .
or alternatively:
a) Contacting the CAI with the linker and reacting to obtain a covalent bond between the CAI and the linker; Contacting the CAI covalently bound to the linker as obtained in step a) with the NIR absorber and reacting to obtain the assembly.
Process according to claim 27 wherein gold nanorods with effective radii of ~ 10 nm and an aspect ratio of ~ 4 are linked to 4- (2- aminoethyl ) benzenesul fonamide by means of an alpha mercapto omega carboxy polyethylene glycol cross linker through a gold sulphur bridge and the amino termination from 4- (2- aminoethyl ) benzenesul fonamide upon amidation.
PCT/IB2014/059490 2013-03-07 2014-03-06 Assembly comprising an absorber of near infrared (nir) light covalently linked to an inhibitor of carbonic anhydrase Ceased WO2014136076A2 (en)

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