WO2014148931A3 - Plant aspartic proteases with deleted psi motifs - Google Patents

Plant aspartic proteases with deleted psi motifs Download PDF

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Publication number
WO2014148931A3
WO2014148931A3 PCT/PT2014/000017 PT2014000017W WO2014148931A3 WO 2014148931 A3 WO2014148931 A3 WO 2014148931A3 PT 2014000017 W PT2014000017 W PT 2014000017W WO 2014148931 A3 WO2014148931 A3 WO 2014148931A3
Authority
WO
WIPO (PCT)
Prior art keywords
aspartic proteases
motifs
psi
deleted
plant aspartic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/PT2014/000017
Other languages
French (fr)
Other versions
WO2014148931A2 (en
Inventor
Carla Sofia Gomes Malaquias de ALMEIDA
Isaura Isabel Gonçalves SIMÕES
Carlos José Fialho Costa FARO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocant Associacao de Transferencia de Tecnologia
Original Assignee
Biocant Associacao de Transferencia de Tecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocant Associacao de Transferencia de Tecnologia filed Critical Biocant Associacao de Transferencia de Tecnologia
Priority to US14/777,847 priority Critical patent/US10196624B2/en
Priority to DK14715461.1T priority patent/DK2976356T3/en
Priority to EP14715461.1A priority patent/EP2976356B1/en
Priority to ES14715461T priority patent/ES2763650T3/en
Priority to CA2942785A priority patent/CA2942785C/en
Priority to BR112015024120A priority patent/BR112015024120A2/en
Publication of WO2014148931A2 publication Critical patent/WO2014148931A2/en
Publication of WO2014148931A3 publication Critical patent/WO2014148931A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/02—Making cheese curd
    • A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
    • A23C19/041—Proteolytic or milk clotting enzymes from plants or vegetables, e.g. papain, ficin, bromelain
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/1203—Addition of, or treatment with, enzymes or microorganisms other than lactobacteriaceae
    • A23C9/1209—Proteolytic or milk coagulating enzymes, e.g. trypsine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to aspartic proteases, and particularly to aspartic proteases for plants. Disclosed are modified plant aspartic proteases, and methods for their manufacture, and uses thereof. Particularly contemplated are the uses of aspartic proteases in clotting milk.
PCT/PT2014/000017 2013-03-19 2014-03-19 Aspartic proteases Ceased WO2014148931A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US14/777,847 US10196624B2 (en) 2013-03-19 2014-03-19 Aspartic proteases
DK14715461.1T DK2976356T3 (en) 2013-03-19 2014-03-19 aspartic proteases
EP14715461.1A EP2976356B1 (en) 2013-03-19 2014-03-19 Aspartic proteases
ES14715461T ES2763650T3 (en) 2013-03-19 2014-03-19 Aspartic proteases
CA2942785A CA2942785C (en) 2013-03-19 2014-03-19 Aspartic proteases
BR112015024120A BR112015024120A2 (en) 2013-03-19 2014-03-19 vegetable aspartic protease, nucleic acid, vector, cell with a modified genome, method for producing a vegetable aspartic protease, use of a vegetable aspartic protease, method for coagulating milk involving exposing milk to a modified vegetable aspartic protease, method of making cheese which exposes milk to a modified vegetable aspartic protease, method for coagulating milk, method for promoting accumulation of a polypeptide of interest in a cell vacuole, and cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1305025.7A GB201305025D0 (en) 2013-03-19 2013-03-19 Aspartic proteases
GB1305025.7 2013-03-19

Publications (2)

Publication Number Publication Date
WO2014148931A2 WO2014148931A2 (en) 2014-09-25
WO2014148931A3 true WO2014148931A3 (en) 2014-12-31

Family

ID=48226676

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/PT2014/000017 Ceased WO2014148931A2 (en) 2013-03-19 2014-03-19 Aspartic proteases

Country Status (9)

Country Link
US (1) US10196624B2 (en)
EP (1) EP2976356B1 (en)
BR (1) BR112015024120A2 (en)
CA (1) CA2942785C (en)
DK (1) DK2976356T3 (en)
ES (1) ES2763650T3 (en)
GB (1) GB201305025D0 (en)
PT (1) PT2976356T (en)
WO (1) WO2014148931A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201305023D0 (en) 2013-03-19 2013-05-01 Biocant Associa O De Transfer Ncia De Tecnologia Aspartic proteases
ES2673702B2 (en) * 2016-12-23 2018-10-05 Universidade De Santiago De Compostela Recombinant strain, Galium verum aspartic protease production method and use in the dairy industry.

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075283A1 (en) * 1999-06-09 2000-12-14 Instituto De Ciencia Aplicada E Tecnologia (Icat) Production by yeasts of aspartic proteinases from plant origin with sheep's, cow's, goat's milk, etc. clotting and proteolytic activity

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT103839B (en) 2007-09-28 2008-10-23 Ecbio Investigacao E Desenvolv PHARMACEUTICAL COMPOSITIONS CONTAINING CIPROSINE ENZYME, AN ASPARTIC PEPTIDASE OF CYNARA CARDUNCULUS, AND ITS INCLUSION IN ANTITUMURIAL FORMULATIONS
GB201305023D0 (en) 2013-03-19 2013-05-01 Biocant Associa O De Transfer Ncia De Tecnologia Aspartic proteases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000075283A1 (en) * 1999-06-09 2000-12-14 Instituto De Ciencia Aplicada E Tecnologia (Icat) Production by yeasts of aspartic proteinases from plant origin with sheep's, cow's, goat's milk, etc. clotting and proteolytic activity

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CURTO PEDRO ET AL: "Establishing the yeast Kluyveromyces lactis as an expression host for production of the saposin-like domain of the aspartic protease cirsin", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 80, no. 1, 1 January 2014 (2014-01-01), pages 86 - 96, XP009178777, ISSN: 1098-5336, [retrieved on 20131011] *
K. TORMAKANGAS: "A Vacuolar Sorting Domain May Also Influence the Way in Which Proteins Leave the Endoplasmic Reticulum", THE PLANT CELL ONLINE, vol. 13, no. 9, 1 September 2001 (2001-09-01), pages 2021 - 2032, XP055125636, ISSN: 1040-4651, DOI: 10.1105/tpc.13.9.2021 *
MAZORRA-MANZANO M A ET AL: "Expression and characterization of the recombinant aspartic proteinase A1 from Arabidopsis thaliana", PHYTOCHEMISTRY, PERGAMON PRESS, GB, vol. 69, no. 13, 1 October 2008 (2008-10-01), pages 2439 - 2448, XP025535878, ISSN: 0031-9422, [retrieved on 20080914], DOI: 10.1016/J.PHYTOCHEM.2008.07.012 *
MILISAVLJEVIC M DJ ET AL: "Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis", JOURNAL OF PLANT PHYSIOLOGY, URBAN UND FISCHER VERLAG, DE, vol. 165, no. 9, 16 June 2008 (2008-06-16), pages 983 - 990, XP022691027, ISSN: 0176-1617, [retrieved on 20071017], DOI: 10.1016/J.JPLPH.2007.03.016 *
PRASAD B D ET AL: "Heterologous expression and characterization of recombinant OsCDR1, a rice aspartic proteinase involved in disease resistance", PROTEIN EXPRESSION AND PURIFICATION, ACADEMIC PRESS, SAN DIEGO, CA, vol. 72, no. 2, 1 August 2010 (2010-08-01), pages 169 - 174, XP027052627, ISSN: 1046-5928, [retrieved on 20100515], DOI: 10.1016/J.PEP.2010.03.018 *
SAMPAIO P N ET AL: "Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, ELSEVIER, AMSTERDAM, NL, vol. 105, no. 4, 1 April 2008 (2008-04-01), pages 305 - 312, XP022672643, ISSN: 1389-1723, [retrieved on 20080401], DOI: 10.1263/JBB.105.305 *
WHITE PAUL C ET AL: "Processing, activity, and inhibition of recombinant cyprosin, an aspartic proteinase from Cardoon (Cynara cardunculus)", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 274, no. 24, 11 June 1999 (1999-06-11), pages 16685 - 16693, XP002149833, ISSN: 0021-9258, DOI: 10.1074/JBC.274.24.16685 *

Also Published As

Publication number Publication date
CA2942785A1 (en) 2014-09-25
ES2763650T3 (en) 2020-05-29
EP2976356B1 (en) 2019-09-25
DK2976356T3 (en) 2020-01-02
GB201305025D0 (en) 2013-05-01
US20160083711A1 (en) 2016-03-24
US10196624B2 (en) 2019-02-05
EP2976356A2 (en) 2016-01-27
CA2942785C (en) 2021-07-13
BR112015024120A2 (en) 2017-10-24
PT2976356T (en) 2020-01-10
WO2014148931A2 (en) 2014-09-25

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