WO2016044604A1

WO2016044604A1 - Carm1 inhibitors and uses thereof

Info

Publication number: WO2016044604A1
Application number: PCT/US2015/050712
Authority: WO
Inventors: Richard Chesworth; Oscar Miguel Moradei; Gideon Shapiro; Lei Jin; Robert E. Babine
Original assignee: Epizyme Inc
Current assignee: Epizyme Inc
Priority date: 2014-09-17
Filing date: 2015-09-17
Publication date: 2016-03-24
Anticipated expiration: 2017-03-17
Also published as: US20170305922A1; EP3193608A1; EP3193608A4; JP2017530959A

Abstract

Provided herein are compounds of Formula (I): and pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof, wherein R1, R2a, R2b, R3 and Ring B are as defined herein, and Ring A is a group of Formula (A-i), (A-ii), or (A-iii): wherein R, R, R, R, and R are as defined herein. Compounds of the present invention are useful for inhibiting CARMl activity. Methods of using the compounds for treating CARMl -mediated disorders are also described.

Description

CARM1 INHIBITORS AND USES THEREOF

RELATED APPLICATIONS

[0001] The present application claims priority under 35 U.S.C. § 119(e) to U.S.

provisional patent application, U.S. S.N. 62/051,872, filed September 17, 2014, the entire contents of which is incorporated herein by reference.

BACKGROUND OF THE INVENTION

[0002] Epigenetic regulation of gene expression is an important biological determinant of protein production and cellular differentiation and plays a significant pathogenic role in a number of human diseases.

[0003] Epigenetic regulation involves heritable modification of genetic material without changing its nucleotide sequence. Typically, epigenetic regulation is mediated by selective and reversible modification (e.g. , methylation) of DNA and proteins (e.g. , histones) that control the conformational transition between transcriptionally active and inactive states of chromatin. These covalent modifications can be controlled by enzymes such as

methyltransferases (e.g., CARM1 (co-activator-associated arginine methyltransferase 1 ; PRMT4)), many of which are associated with specific genetic alterations that can cause human disease.

[0004] Disease-associated chromatin-modifying enzymes play a role in diseases such as proliferative disorders, autoimmune disorders, muscular disorders, and neurological disorders. Thus, there is a need for the development of small molecules that are capable of inhibiting the activity of CARM1.

DETAILED DESCRIPTION OF CERTAIN EMBODIMENTS

[0005] CARM1 is an attractive target for modulation given its role in the regulation of diverse biological processes. It has now been found that compounds described herein, and pharmaceutically acceptable salts and compositions thereof, are effective as inhibitors of CARM1. Such compounds have the general Formula (I):

and pharmaceutically acceptable salts thereof, and pharmaceutical compositions thereof; wherein R¹, R^2a, R^2b, R³, Ring A and Ring B are as defined herein.

[0006] Pharmaceutical compositions are further provided comprising a compound described herein (e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof) and, optionally, a pharmaceutically acceptable excipient.

[0007] In certain embodiments, compounds described herein inhibit the activity of CARMl . In certain embodiments, methods of inhibiting CARMl are provided which comprise contacting CARMl with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof. The CARMl may be purified or crude, and may be present in a cell, tissue, or a subject. Thus, such methods encompass inhibition of CARMl activity both in vitro and in vivo. In certain embodiments, the CARMl is wild-type CARMl . In certain embodiments, the CARMl is overexpressed. In certain embodiments, the CARMl is a mutant. In certain embodiments, the CARMl is in a cell. In certain embodiments, the CARMl is in a tissue. In certain embodiments, the CARMl is in a biological sample. In certain embodiments, the CARMl is in an animal, e.g., a human. In some embodiments, the CARMl is expressed at normal levels in a subject, but the subject would benefit from

CARMl inhibition (e.g., because the subject has one or more mutations in an CARMl substrate that causes an increase in methylation of the substrate with normal levels of CARMl). In some embodiments, the CARMl is in a subject known or identified as having abnormal CARMl activity (e.g., overexpression). In some embodiments, the CARMl is in a subject known or identified as having aberrant CARMl activity. In some embodiments, a provided compound is selective for CARMl over other methyltransferases. In certain embodiments, a provided compound is at least about 10-fold selective, at least about 20-fold selective, at least about 30-fold selective, at least about 40-fold selective, at least about 50- fold selective, at least about 60-fold selective, at least about 70-fold selective, at least about 80-fold selective, at least about 90-fold selective, or at least about 100-fold selective relative to one or more other methyltransferases. [0008] In certain embodiments, methods of modulating gene expression or activity in a cell are provided which comprise contacting a cell with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical

composition thereof. In certain embodiments, the cell is cultured in vitro. In certain embodiments, cell is in an animal, e.g., a human.

[0009] In certain embodiments, methods of modulating transcription in a cell are provided which comprise contacting a cell with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof. In certain embodiments, the cell is cultured in vitro. In certain embodiments, the cell is in an animal, e.g., a human.

[0010] In some embodiments, methods of treating a CARMl -mediated disorder are provided which comprise administering to a subject suffering from a CARMl -mediated disorder an effective amount of a compound described herein {e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof), or a pharmaceutical composition thereof. In certain embodiments, the CARMl -mediated disorder is a proliferative disorder. In certain embodiments, compounds described herein are useful for treating cancer. In certain embodiments, compounds described herein are useful for treating breast cancer or prostate cancer. In certain embodiments, the CARMl -mediated disorder is a metabolic disorder.

[0011] Compounds described herein are also useful for the study of CARMl in biological and pathological phenomena, the study of intracellular signal transduction pathways mediated by CARMl, and the comparative evaluation of new CARMl inhibitors.

[0012] This application refers to various issued patent, published patent applications, journal articles, and other publications, all of which are incorporated herein by reference.

[0013] Definitions of specific functional groups and chemical terms are described in more detail below. The chemical elements are identified in accordance with the Periodic Table of the Elements, CAS version, Handbook of Chemistry and Physics, 75^th Ed., inside cover, and specific functional groups are generally defined as described therein. Additionally, general principles of organic chemistry, as well as specific functional moieties and reactivity, are described in Thomas Sorrell, Organic Chemistry, University Science Books, Sausalito, 1999; Smith and March, March 's Advanced Organic Chemistry, 5^th Edition, John Wiley & Sons, Inc., New York, 2001; Larock, Comprehensive Organic Transformations, VCH Publishers, Inc., New York, 1989; and Carruthers, Some Modern Methods of Organic Synthesis, 3^rd Edition, Cambridge University Press, Cambridge, 1987. [0014] Compounds described herein can comprise one or more asymmetric centers, and thus can exist in various isomeric forms, e.g., enantiomers and/or diastereomers. For example, the compounds described herein can be in the form of an individual enantiomer, diastereomer or geometric isomer, or can be in the form of a mixture of stereoisomers, including racemic mixtures and mixtures enriched in one or more stereoisomer. Isomers can be isolated from mixtures by methods known to those skilled in the art, including chiral high pressure liquid chromatography (HPLC) and the formation and crystallization of chiral salts; or preferred isomers can be prepared by asymmetric syntheses. See, for example, Jacques et al., Enantiomers, Racemates and Resolutions (Wiley Interscience, New York, 1981); Wilen et al, Tetrahedron 33:2725 (1977); Eliel, Stereochemistry of Carbon Compounds (McGraw- Hill, NY, 1962); and Wilen, Tables of Resolving Agents and Optical Resolutions p. 268 (E.L. Eliel, Ed., Univ. of Notre Dame Press, Notre Dame, IN 1972). The present disclosure additionally encompasses compounds described herein as individual isomers substantially free of other isomers, and alternatively, as mixtures of various isomers.

[0015] Unless otherwise stated, structures depicted herein are also meant to include compounds that differ only in the presence of one or more isotopically enriched atoms. For example, compounds having the present structures except for the replacement of hydrogen by deuterium or tritium, replacement of 19 F with 18 F, or the replacement of a carbon by 13 C or ¹⁴C are within the scope of the disclosure. Such compounds are useful, for example, as analytical tools or probes in biological assays.

[0016] When a range of values is listed, it is intended to encompass each value and subrange within the range. For example "Ci_₃ alkyl" is intended to encompass, C_ls C₂, C₃, Ci_₃, Ci_2, and C₂-₃ alkyl.

[0017] "Alkyl" refers to a radical of a straight-chain or branched saturated hydrocarbon group having from 1 to 3 carbon atoms ("Ci_₃ alkyl"). In some embodiments, an alkyl group has 1 to 2 carbon atoms ("Ci_₂ alkyl"). In some embodiments, an alkyl group has 1 carbon atom ("Ci alkyl"). Examples of Ci_₃ alkyl groups include methyl (Ci), ethyl (C₂), n-propyl (C₃),and isopropyl (C₃). Alkyl groups may be substituted or unsubstituted as described herein.

[0018] "Haloalkyl" refers to an alkyl group, as defined herein, substituted with one or more halogen atoms, e.g., 1, 2, 3, 4, 5, 6, or 7 halogen atoms independently selected from the group consisting of fluoro, bromo, chloro, and iodo. Haloalkyl encompasses perhaloalkyl as defined herein. "Perhaloalkyl" refers to a substituted alkyl group as defined herein wherein all of the hydrogen atoms are independently replaced by a halogen. In some embodiments, at least one of the hydrogen atoms is replaced with fluoro. In some embodiments, at least one of the hydrogen atoms is replaced with chloro. Examples of perhaloalkyl groups include - CF₃, -CF₂CF₃, -CF₂CF₂CF₃, -CCI3, -CFCI2, -CF₂C1, and the like. Examples of haloalkyl groups include all of the aforementioned perhaloalkyl groups, as well as groups such as - CH₂F, -CHF₂, -CH2CI, CHC1₂, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, - CH2CH2CI, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH2CH2CH2CI, -CH(CH₃)CHF₂, -CH(CH₃)CF₃, and the like.

[0019] "Alkenyl" refers to a radical of a straight-chain or branched hydrocarbon group having from 2 to 3 carbon atoms and one carbon-carbon double bond ("C₂_₃ alkenyl"). In some embodiments, an alkenyl group has 2 carbon atoms ("C₂ alkenyl"). In some

embodiments, an alkenyl group has 3 carbon atoms ("C₃ alkenyl"). Examples of C₂_₃ alkenyl groups include ethenyl (C₂), 1-propenyl (C₃), and 2-propenyl (C₃). Alkenyl groups may be substituted or unsubstituted as described herein.

[0020] "Alkynyl" refers to a radical of a straight-chain or branched hydrocarbon group having from 2 to 3 carbon atoms and one carbon-carbon triple bond ("C₂_₃ alkynyl"). In some embodiments, an alkynyl group has 2 carbon atoms ("C₂ alkynyl"). In some embodiments, an alkynyl group has 3 carbon atoms ("C₃ alkynyl"). Examples of C₂_₃ alkynyl groups include, without limitation, ethynyl (C₂), 1-propynyl (C₃), and 2-propynyl (C₃). Alkynyl groups may be substituted or unsubstituted as described herein.

[0021] "Carbocyclyl" or "carbocyclic" refers to a radical of a non-aromatic monocyclic hydrocarbon group having from 3 to 6 ring carbon atoms ("C₃_₆ carbocyclyl") and zero heteroatoms in the non-aromatic ring system. In some embodiments, a carbocyclyl group has 3 to 4 ring carbon atoms ("C₃^ carbocyclyl"). In some embodiments, a carbocyclyl group has 3 to 5 ring carbon atoms ("C₃_5 carbocyclyl"). In some embodiments, a

carbocyclyl group has 4 to 6 ring carbon atoms ("C4_₆ carbocyclyl"). In some embodiments, a carbocyclyl group has 5 to 6 ring carbon atoms ("C₅_6 carbocyclyl"). Exemplary C₃_₆ carbocyclyl groups include, without limitation, cyclopropyl (C₃), cyclopropenyl (C₃), cyclobutyl (C₄), cyclobutenyl (C₄), cyclopentyl (C₅), cyclopentenyl (C₅), cyclohexyl (C₆), cyclohexenyl (C₆), cyclohexadienyl (C₆), and the like.

[0022] "Heterocyclyl" or "heterocyclic" refers to a radical of a 4-6 membered monocyclic non-aromatic ring system having ring carbon atoms and 1, 2, or 3 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("4-6 membered heterocyclyl"). In heterocyclyl groups that contain one or more nitrogen atoms, the point of attachment can be a carbon or nitrogen atom, as valency permits. In some embodiments, a heterocyclyl group is a 4-membered monocyclic non-aromatic ring system having ring carbon atoms and 1 ring heteroatom, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("4-membered heterocyclyl"). In some embodiments, a heterocyclyl group is a 5 - membered monocyclic non-aromatic ring system having ring carbon atoms and 1, 2, or 3 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("5- membered heterocyclyl"). In some embodiments, a heterocyclyl group is a 6- membered monocyclic non-aromatic ring system having ring carbon atoms and 1, 2, or 3 ring heteroatoms, wherein each heteroatom is independently selected from nitrogen, oxygen, and sulfur ("6- membered heterocyclyl"). Exemplary 4-membered heterocyclyl groups containing one heteroatom include, without limitation, azetidinyl, oxetanyl, and thietanyl. Exemplary 5-membered heterocyclyl groups containing one heteroatom include, without limitation, tetrahydrofuranyl, dihydrofuranyl, tetrahydrothiophenyl, dihydrothiophenyl, pyrrolidinyl, dihydropyrrolyl, pyrrolyl-2,5-dione, and pyrrolidin-2-one. Exemplary 5-membered heterocyclyl groups containing two heteroatoms include, without limitation, dioxolanyl, oxasulfuranyl, disulfuranyl, and oxazolidin-2-one. Exemplary 5-membered heterocyclyl groups containing three heteroatoms include, without limitation, triazolinyl, oxadiazolinyl, and thiadiazolinyl. Exemplary 6- membered heterocyclyl groups containing one heteroatom include, without limitation, piperidinyl, tetrahydropyranyl, dihydropyridinyl, and thianyl. Exemplary 6-membered heterocyclyl groups containing two heteroatoms include, without limitation, piperazinyl, morpholinyl, dithianyl, and dioxanyl. Exemplary 6-membered heterocyclyl groups containing three heteroatoms include, without limitation, triazinanyl.

[0023] Affixing the suffix "-ene" to a group indicates the group is a divalent moiety, e.g., alkylene is the divalent moiety of alkyl, alkenylene is the divalent moiety of alkenyl, alkynylene is the divalent moiety of alkynyl, as defined herein.

[0024] In general, the term "substituted" means that at least one hydrogen present on a group {e.g., a carbon or nitrogen atom) is replaced with a substituent as defined herein and results in a stable compound, e.g., a compound which does not spontaneously undergo transformation such as by rearrangement, cyclization, elimination, or other reaction. Unless otherwise indicated, a "substituted" group may have a substituent at one or more substitutable positions of the group, and when more than one position in any given structure is substituted, the substituent may be the same or different at each position.

[0025] "Halo" or "halogen" refers to fluorine (fluoro, -F), chlorine (chloro, -CI), bromine (bromo, -Br), or iodine (iodo, -I). [0026] "Pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and other animals without undue toxicity, irritation, allergic response, and the like, and are

commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al. describe pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences (1977) 66: 1-19. Pharmaceutically acceptable salts of the compounds describe herein include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid, or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2- naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, /?-toluenesulfonate, undecanoate, valerate salts, and the like. Salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N⁺(Ci_₄alkyl)₄ salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, quaternary salts.

[0027] A "subject" to which administration is contemplated includes, but is not limited to, humans (e.g., a male or female of any age group, e.g., a pediatric subject (e.g, infant, child, adolescent) or adult subject (e.g., young adult, middle-aged adult or senior adult)) and/or other non-human animals, for example, non-human mammals (e.g., primates (e.g., cynomolgus monkeys, rhesus monkeys); commercially relevant mammals such as cattle, pigs, horses, sheep, goats, cats, and/or dogs), birds (e.g., commercially relevant birds such as chickens, ducks, geese, and/or turkeys), rodents (e.g., rats and/or mice), reptiles, amphibians, and fish. In certain embodiments, the non-human animal is a mammal. The non-human animal may be a male or female at any stage of development. A non-human animal may be a transgenic animal. [0028] "Condition," "disease," and "disorder" are used interchangeably herein.

[0029] "Treat," "treating" and "treatment" encompasses an action that occurs while a subject is suffering from a condition which reduces the severity of the condition or retards or slows the progression of the condition ("therapeutic treatment"). "Treat," "treating" and "treatment" also encompasses an action that occurs before a subject begins to suffer from the condition and which inhibits or reduces the severity of the condition ("prophylactic treatment").

[0030] An "effective amount" of a compound refers to an amount sufficient to elicit the desired biological response, e.g., treat the condition. As will be appreciated by those of ordinary skill in this art, the effective amount of a compound described herein may vary depending on such factors as the desired biological endpoint, the pharmacokinetics of the compound, the condition being treated, the mode of administration, and the age and health of the subject. An effective amount encompasses therapeutic and prophylactic treatment.

[0031] A "therapeutically effective amount" of a compound is an amount sufficient to provide a therapeutic benefit in the treatment of a condition or to delay or minimize one or more symptoms associated with the condition. A therapeutically effective amount of a compound means an amount of therapeutic agent, alone or in combination with other therapies, which provides a therapeutic benefit in the treatment of the condition. The term "therapeutically effective amount" can encompass an amount that improves overall therapy, reduces or avoids symptoms or causes of the condition, or enhances the therapeutic efficacy of another therapeutic agent.

[0032] A "prophylactically effective amount" of a compound is an amount sufficient to prevent a condition, or one or more symptoms associated with the condition or prevent its recurrence. A prophylactically effective amount of a compound means an amount of a therapeutic agent, alone or in combination with other agents, which provides a prophylactic benefit in the prevention of the condition. The term "prophylactically effective amount" can encompass an amount that improves overall prophylaxis or enhances the prophylactic efficacy of another prophylactic agent.

[0033] As used herein, the term "methyltransferase" represents transferase class enzymes that are able to transfer a methyl group from a donor molecule to an acceptor molecule, e.g. , an amino acid residue of a protein or a nucleic base of a DNA molecule. Methytransferases typically use a reactive methyl group bound to sulfur in S-adenosyl methionine (SAM) as the methyl donor. In some embodiments, a methyltransferase described herein is a protein methyltransferase. In some embodiments, a methyltransferase described herein is a histone methyltransferase. Histone methyltransferases (HMT) are histone -modifying enzymes, (including histone-lysine N-methyltransferase and histone-arginine N-methyltransferase), that catalyze the transfer of one or more methyl groups to lysine and arginine residues of histone proteins. In certain embodiments, a methyltransferase described herein is a histone-arginine N-methyltransferase.

[0034] As generally described above, provided herein are compounds useful as CARMl inhibitors. In some embodiments, the present disclosure provides a compound of Formula (I):

or pharmaceutically acceptable salt thereof;

wherein:

R¹ is hydrogen, -CHO, or unsubstituted Ci_3alkyl;

each instance of R^2a and R^2b is independently hydrogen, halogen, unsubstituted Ci_ ₃alkyl, or Ci_₃haloalkyl;

R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, or halogen;

Ring A is of formula (A-i), (A-ii), or (A-iii):

wherein:

each instance of R^A1 and R^ is independently unsubstituted Ci_₃alkyl, Ci_ haloalkyl, or unsubstituted cyclopropyl;

R^ is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN;

R^A4 is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN; and R^A5 is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl;

Ring B is any one of formula (i) to (xxviii):

(xxii) (xxiii) (xxiv)

(xxv) (xxvi) (xxvii)

(xxviii) (xxix) wherein:

q is 1 , 2, or 3 and w is 1 ; or q is 2 and w is 0 or 2; x is 1 and y is 1 or 2;

n is 0, 1 , or 2;

Li is -NH-, substituted or unsubstituted C₂alkylene, substituted or unsubstituted C₂alkenylene, or substituted or unsubstituted C₂alkynylene; R^N1 is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^N1A, -C(=0)N(R^N1A)(R^N1B), -C(=0)OR^N1A, or -S(0)₂R^N1A; wherein:

R^N1A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl;

R^N1B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6- membered heterocyclyl; or

R^N1A and R^N1B are joined to form a substituted or unsubstituted 4- to 6- membered heterocyclyl; or

each instance of R^N2 and R^B8 is independently substituted or unsubstituted Ci_ ₃alkyl or Ci_₃haloalkyl, or R^N2 and R^B8 are joined to form a substituted or

unsubstituted 5- to 6-membered ring;

R^B1 is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, -CN, - OR^B1B, -SR^B1B, -N(R^B1A)(R^B1B), substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^B1A, - C(=0)N(R^B1A)(R^B1B), -C(=0)OR^B1A, -S(0)₂R^B1A, -OC(=0)R^B1A, - OC(=0)N(R^B1A)(R^B1B), -OC(=0)OR^B1A, -NR^BIBC(=0)R^B1A, - NR^BIBC(=0)N(R^B1A)(R^B1B), or -NR^BIBC(=0)OR^B1A;

wherein:

R^B1A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl; and

R^B1B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6- membered heterocyclyl; or

R^B1A and R^B1B are joined to form a substituted or unsubstituted 4- to 6- membered heterocyclyl;

R^B2 is hydrogen, halogen, -OR^B2A, substituted or unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, wherein R^B2A i_s substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl; or R^B1 and R^B2 are joined to form a substituted or unsubstituted 4- to 6- membered heterocyclyl;

each instance of R is independently hydrogen, unsubstituted Ci_₃alkyl, or Ci_

₃haloalkyl, provided at least one instance of R B3 is hydrogen;

each instance of R^B4, R^B5, R^B6, and R^B7 is independently hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, -CN, -OR^B4B, -SR^B4B, - N(R^B4A)(R^B4B), substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^B4A, -C(=0)N(R^B4A)(R^B4B), - C(=0)OR^B4A, -S(0)₂R^B4A, -OC(=0)R^B4A, -OC(=0)N(R^B4A)(R^B4B), -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)OR^B4A;

wherein:

R^B4A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl; and

R^B4B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6- membered heterocyclyl; or

R^B4A and R^B4B are joined to form a substituted or unsubstituted 4- to 6- membered heterocyclyl;

and

wherein = represents a single or double bond; and

G

further wherein represents a single or double bond or G is -CH₂-;

wherein each instance of substituted independently refers to substitution with 1, 2, or 3 R groups, as valency permits,

and wherein:

each instance of R is independently unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, -CN, -OR^clB, -SR^C1B, -N(R^C1A)(R^C1B), -C(=0)R^C1A, -C(=0)N(R^C1A)(R^C1B), -C(=0)OR^clA, - S(0)₂R^C1A, -OC(=0)R^clA, -OC(=0)N(R^clA)(R^clB), -OC(=0)OR^clA, -NR^CIBC(=0)R^C1A, - NR^CIBC(=0)N(R^C1A)(R^C1B), or -NR^CIBC(=0)OR^clA;

wherein:

R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_6carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups; or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and R is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C3_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; or

R"^1A and R"^1D are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and

wherein:

each instance of R is independently halogen, -CN, -OR , unsubstituted Ci_3 alkyl, or Ci_₃haloalkyl, wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl.

[0035] In certain embodiments, the compound of Formula (I) is a stereoisomer of Formula:

or a pharmaceutically acceptable salt thereof.

[0036] In certain embodiments, the compound of Formula (I) is a stereoisomer of Formula:

or a pharmaceutically acceptable salt thereof.

(I) Groups R¹, R², and R³

[0037] As generally defined herein, R¹ is hydrogen, -CHO, or unsubstituted Ci_₃alkyl.

[0038] In certain embodiments, R¹ is hydrogen. In certain embodiments, R¹ is -CHO. In certain embodiments, R¹ is unsubstituted Ci_₃alkyl, i.e. , unsubstituted Ci alkyl (-C¾), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[0039] Furthermore, as generally defined herein, each instance of R^2a and R^2b is independently hydrogen, halogen, unsubstituted Ci_₃alkyl, or C^haloalkyl. [0040] In certain embodiments, at least one instance of R^2a and R^2b is hydrogen. In certain embodiments, each instance of R^2a and R^2b is hydrogen.

[0041] In certain embodiments, at least one instance of R^2a and R^2b is halogen, i.e., at least one instance of R^2a and R^2b is -F, -CI, -Br, or -I. In certain embodiments, R^2a is halogen and R^2b is halogen, i.e., each instance of R^2a and R^2b is independently -F, -CI, -Br, or -I. In certain embodiments, at least one instance of R^2a and R^2b is -F or -CI. In certain

embodiments, R^2a is -F or -CI. In certain embodiments, R^2b is -F or -CI. In certain

2 2b 2 2b

embodiments, R ^a is -CI and R is -CI. In certain embodiments, R ^a is -F and R is -F.

[0042] In certain embodiments, at least one instance of R^2a and R^2b is unsubstituted Ci_ ₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH ), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, at least one instance of R^2a and R^2b is -CH₃.

[0043] In certain embodiments, at least one instance of R^2a and R^2b is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, at least one instance of R^2a and R^2b is -CF₃. In certain embodiments, R^2a is -CF₃. In certain embodiments, R^2b is -CF₃.

[0044] In certain embodiments, R^2b is hydrogen and R^2a is halogen, unsubstituted Ci_ ₃alkyl, or Ci_₃haloalkyl. In certain embodiments, R^2b is hydrogen and R^2a is halogen, i.e., R^2b is hydrogen and R^2a is -F, -CI, -Br, or -I. In certain embodiments, R^2b is hydrogen and R^2a is unsubstituted Ci_₃ alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^2b is hydrogen and R^2a is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, - CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, - CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, - CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, R^2b is hydrogen and R^2a is -CI. In certain embodiments, R^2b is hydrogen and R^2a is -F. In certain embodiments, R^2b is hydrogen and R^2a is -CF₃.

[0045] In certain embodiments, R^2a is hydrogen and R^2b is halogen, unsubstituted Ci_ ₃alkyl, or Ci_₃haloalkyl. In certain embodiments, R^2a is hydrogen and R^2b is halogen, i.e., R^2a is hydrogen and R^2b is -F, -CI, -Br, or -I. In certain embodiments, R^2a is hydrogen and R^2b is unsubstituted Ci_₃ alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH ), or unsubstituted C₃ alkyl (-CH₂CH₂CH or -CH(CH )₂). In certain embodiments, R^2a is hydrogen and R^2b is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, -CFCI₂, -CF₂C1, -CH₂F, - CHF₂, -CH₂CI, CHCI₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, - CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, - CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, R^2a is hydrogen and R^2b is -CF₃.

[0046] In certain embodiments, R^2a is hydrogen and R^2b is -CI. In certain embodiments,

2 2b 2 2b

R ^a is hydrogen and R is -F. In certain embodiments, R ^a is hydrogen and R is -CF₃.

[0047] Furthermore, as generally defined herein, R is unsubstituted Ci_₃alkyl, Ci_

3haloalkyl, or halogen. In certain embodiments, R is unsubstituted Ci_₃ alkyl, i.e.,

unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (- CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R is Ci_₃haloalkyl, e.g., C_\ haloalkyl (-CF₃, -CCI₃, -CFCI₂, -CF₂CI, -CH₂F, -CHF₂, -CH₂CI, CHCI₂), C₂ haloalkyl (-CF₂CF₃, - CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (- CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂CI, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

3 3

embodiments, R is -CH₃. In certain embodiments, R is halogen, i.e., -F, -CI, -Br, or -I. In certain embodiments, R is -F or -CI.

[0048] Various combinations of R^2a, R^2b, and R³ are contemplated herein.

[0049] For example, in certain embodiments, each of R^2a and R³ is the same group. In certain embodiments, R^2a and R³ are different groups. In certain embodiments, each of R^2a and R³ is halogen, e.g., R^2a is -CI and R³ is -CI, or R^2a is -F and R³ is -F, or R^2a is -CI and

3 2 3 2 3

R is -F, or R ^a is -F and R is -CI. In certain embodiments, R ^a is halogen and R is unsubstituted C_1-3alkyl, e.g., wherein R^2a is -CI and R³ is -CH₃, or R^2a is -F and R³ is -CH₃. In certain embodiments, R^2a is Ci_₃haloalkyl and R³ is unsubstituted Ci_₃alkyl, e.g., R^2a is - CF₃ and R³ is -CH₃. In certain embodiments, R^2a is hydrogen and R³ is unsubstituted Ci_ ₃alkyl, e.g., wherein R^2a is hydrogen and R³ is -CH₃.

[0050] In certain embodiments, R^2a is halogen {e.g., -F or -CI), R^2b is hydrogen, and R³ is unsubstituted Ci_₃alkyl {e.g., -CH ). In certain embodiments, R^2a is -CI, R^2b is hydrogen, and R³ is -CH₃, or R^2a is -F, R^2b is hydrogen, and R³ is -CH₃, to provide a compound of

Formulae:

or a pharmaceutically acceptable salt thereof.

[0051] In certain embodiments, R^2a is halogen (e.g., -F or -CI), R^2b is hydrogen, and R³ is halogen (e.g., -F or -CI). In certain embodiments, R^2a is -CI, R^2b is hydrogen, and R³ is -CI, or R^2a is -F R^2b is hydrogen, and R³ is -F, to provide a compound of Formulae:

or a pharmaceutically acceptable salt thereof.

[0052] In certain embodiments, R^2a is Ci_₃haloalkyl (e.g., -CF₃), R^2b is hydrogen, and R³ is unsubstituted Ci_₃alkyl (e.g., -CH ). In certain embodiments, R^2a is -CF , R^2b is hydrogen, and R is -CH₃ to provide a com ound of Formula:

or a pharmaceutically acceptable salt thereof.

[0053] In certain embodiments, each of R^2a and R^2b is hydrogen, and R³ is unsubstituted Ci_₃alkyl (e.g., -CH₃). In certain embodiments, R^2a is hydrogen, R^2b is hydrogen, and R³ is - CH to provide a compound of Formula:

or a pharmaceutically acceptable salt thereof.

[0054] In certain embodiments, each of R^2a, R^2b, and R³ is halogen (e.g., -F or -CI). For

2 2b 3 2 2b 3 example, in certain embodiments, each of R ^a, R , and R is -CI, or each of R ^a, R , and R is -F to provide a compound ofFormulae:

or a pharmaceutically acceptable salt thereof.

[0055] In certain embodiments, each of R^2a and R^2b is independently halogen (e.g., -F or -CI), R³ is unsubstituted Ci_₃alkyl (e.g., -CH₃). In certain embodiments, R^2a is -CI, R^2b is -CI, and R³ is -CH or R^2a is -F, R^2b is -F, and R³ is -CH₃, provide a compound of Formulae:

[0056] As generally defined herein, Ring A is of formula (A-i), (A-ii), or (A-iii):

wherein:

each instance of R^A1 and R^ is independently unsubstituted Ci_₃alkyl or Ci_

₃haloalkyl;

R^ is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN; R is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN; and

R^A5 is unsubstituted C_halky! or Ci_₃haloalkyl.

(II) Ring A

[0057] In certain embodiments, Ring A is of Formula (A-i):

wherein each instance of R^A1 a anndd RR^ iiss iinnddependently unsubstituted C^alkyl, Ci_₃haloalkyl, or unsubstituted cyclopropyl.

[0058] In certain embodiments, at least one instance of R^A1 and R^ is unsubstituted Ci_ 3alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, at least one of R^A1 and R^ is -CH₃. In certain embodiments, at least one of R^A1 and R^ is -CH₂CH₃.

[0059] In certain embodiments, at least one instance of R^A1 and R^ is Ci_3haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, at least one instance of R^A1 and R^ -CF₃.

[0060] In certain embodiments, at least one of R^A1 and R^ is unsubstituted cyclopropyl.

[0061] In certain embodiments, R^A1 and R^ are the same group, e.g., in certain embodiments, R^A1 and R^ are each -CH₃. However, in certain embodiments, R^A1 and R^ are different groups, e.g., in certain embodiments, R^A1 is -CH₃ and R^ is -CH₂CH₃, or in certain embodiments, R^A1 is -CH₂CH₃ and R^ is -CH₃, or in certain embodiments, R^A1 is unsubstituted cyclopropyl and R^ is -CH_3i or in certain embodiments, R^ is unsubstituted cyclopropyl and R^A1 is -CH₃.

[0062] In certain embodiments, Ring A is selected from the group consisting of:

[0063] In certain embodiments, Ring A is of Formula (A-ii):

[0064] In certain embodiments, R is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or - CH(CH₃)₂). In certain embodiments, R^ is Ci_₃haloalkyl, e.g., C\ haloalkyl Ci haloalkyl (- CF₃, -CCI3, -CFCI2, -CF2CI, -CH₂F, -CHF₂, -CH2CI, CHCI2), C₂ haloalkyl (-CF₂CF₃, - CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (- CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂CI, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, R^ is halogen, i.e., -F, -CI, -Br, or -I. In certain embodiments, R^ is -CN. In certain embodiments, R^ is -CN provided R^A4 is is not also -CN.

[0065] In certain embodiments, R^A4 is hydrogen. In certain embodiments, R^A4 is unsubstituted Ci_₃ alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^A4 is Ci_ ₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHCI₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, - CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or - CH(CH )CF ). In certain embodiments, R^A4 is halogen, i.e., -F, -CI, -Br, or -I. In certain embodiments, R^A4 is -CN. In certain embodiments, R^A4 is -CN provided R^ is is not also - CN.

[0066] In certain embodiments, R^A5 is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl

(-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -

CH(CH )₂). In certain embodiments, R^A5 is Ci_₃haloalkyl, e.g., C_\ haloalkyl (-CF , -CC1₃, -

CFC1₂, -CF₂CI, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -

CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -

CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, -

CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃).

[0067] Various combinations of R^, R^A4, and R^A5 are contemplated herein.

[0068] For example, in certain embodiments, R^ is halogen, -CN, unsubstituted Ci_₃ alkyl, or Ci_₃haloalkyl, and R^A4 is hydrogen. In certain embodiments, R^ is halogen (i.e., -F, -CI, -

Br, or -I), and R^A4 is hydrogen. In certain embodiments, R^ is -CN and R^A4 is hydrogen. In certain embodiments, R is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃) and R is hydrogen. Furthermore, in certain embodiments, in any of the above recited instances, R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[0069] In certain embodiments, R^ is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl and R^A4 is halogen or -CN. In certain embodiments, R^A4 is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl and R^ is halogen or -CN. In certain embodiments, R^ is unsubstituted Ci_₃alkyl (e.g., -CH , - CH₂CH₃) and R^A4 is halogen (i.e., -F, -CI, -Br, or -I). In certain embodiments, R^ is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃) and R^A4 is -CN. Furthermore, in certain embodiments, in any of the above recited instances, R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH , -CH₂CH₃).

[0070] In certain embodiments, Ring A is selected from the group consisting of:

[0072] In certain embodiments, R^ is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or - CH(CH₃)₂). In certain embodiments, R is Ci_₃haloalkyl, e.g., C_\ haloalkyl (-CF , -CC1₃, - CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, - CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, R^ is halogen, i.e., -F, -CI, -Br, or -I.

[0073] In certain embodiments, R^A5 is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or - CH(CH )₂). In certain embodiments, R^A5 is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF , -CC1₃, - CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, - CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃).

[0074] Various combinations of R^ and R^A5 are contemplated herein.

[0075] For example, in certain embodiments, R^ is halogen, -CN, unsubstituted Ci_₃ alkyl, or Ci_₃haloalkyl, and R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). In certain embodiments, R^ is halogen (i.e., -F, -CI, -Br, or -I), and R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH , -CH₂CH ). In certain embodiments, R^ is -CN and R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). In certain embodiments, R^ is unsubstituted Ci_₃alkyl (e.g., -CH₃, - CH₂CH₃) and R^A5 is unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). In certain embodiments, R^ and R^ are the same group. In certain embodiments, R^ and R^A5 are different groups.

[0076] In certain embodiments, Ring A is:

[0077] Various combinations of Ring A, R¹, R^2a, and R^2b, are contemplated herein.

[0078] For example, in certain embodiments, wherein Ring A is of Formula (A-i), (A-ii), or (A-iii), R¹ is -CH , and each R^2a and R^2b is hydrogen, provided is a compound of Formula:

or

or a pharmaceutically acceptable salt thereof.

[0079] In certain embodiments, wherein Ring A is of Formula (A-i), (A-ii), or (A-iii), R¹ is -CH R^2a is -CI, and R^2b is hydrogen, provided is a compound of Formula:

or a pharmaceutically acceptable salt thereof.

[0080] In certain embodiments, wherein Ring A is of Formula (A-i), (A-ii), or (A-iii), R¹ is -CH₃, R^2a is -F, and R^2b is hydrogen, provided is a compound of Formulae:

or

or a pharmaceutically acceptable salt thereof.

[0081] In certain embodiments, wherein Ring A is of Formula (A-i), (A-ii), or (A-iii), R¹ is -CH R^2a is -CF₃, and R^2b is hydrogen, provided is a compound of Formulae:

or a pharmaceutically acceptable salt thereof.

[0082] In certain embodiments, wherein Ring A is of Formula (A-i), (A-ii), or (A-iii), R¹ is -CH₃, R^2a is -CI, and R^2b is -CI, provided is a compound of Formulae:

or

or a pharmaceutically acceptable salt thereof.

(Ill) Ring B Groups: Substitution by R^C1 and R⁰¹

[0083] It is generally understood, as described herein, that each instance of "substituted" preceding a group refers to a group, e.g., substituted C₂alkylene, substituted C₂alkenylene, or substituted C₂alkynylene in the instance of L_ls and substituted Ci_₃alkyl, substituted C3-6 carbocyclyl, substituted 4- to 6-membered heterocyclyl, and substituted 5- to 6-membered ring, in the instance of various Ring B recitations, refers to a group substituted with 1, 2, or 3 R groups, as valency permits. In certain embodiments, such groups are substituted with 1 or 2 R^C1 groups.

[0084] As generally defined herein, each instance of R is independentlyunsubstituted Ci_ ₃alkyl, Ci_₃haloalkyl, halogen, -CN, -OR^clB, -SR^C1B, -N(R^C1A)(R^C1B), -C(=0)R^C1A, - C(=0)N(R^C1A)(R^C1B), -C(=0)OR^clA, -S(0)₂R^C1A, -OC(=0)R^clA, -OC(=0)N(R^clA)(R^clB), - OC(=0)OR^clA, -NR^CIBC(=0)R^C1A, -NR^CIBC(=0)N(R^C1A)(R^C1B), or -NR^CIBC(=0)OR^clA, wherein R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and R^C1B is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_ ₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered

heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; or R^C1A and R^C1B are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and wherein each instance of R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl.

[0085] By way of example, in certain embodiments, substituted Ci_₃alkyl refers to a Ci_ ₃alkyl substituted with 1, 2, or 3 R groups, as valency permits, selected from the group consisting of halogen, -CN, -OR^clB, -SR^C1B, -N(R^C1A)(R^C1B), -C(=0)R^C1A, - C(=0)N(R^C1A)(R^C1B), -C(=0)OR^clA, -S(0)₂R^C1A, -OC(=0)R^clA, -OC(=0)N(R^clA)(R^clB), - OC(=0)OR^clA, -NR^CIBC(=0)R^B1A, -NR^CIBC(=0)N(R^C1A)(R^C1BB), and -NR^CIBC(=0)OR^clA In certain embodiments, any recited instance of substituted Ci_₃alkyl refers to a Ci_₃alkyl substituted with 1 or 2 R groups selected from the group consisting of -CN, -OR , - N(R^C1A)(R^C1B), -C(=0)N(R^C1A)(R^C1B), and -C(=0)OR^clA

[0086] In certain embodiments, at least one instance of R is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (- CH₂CH₂CH₃ or -CH(CH₃)₂). Such embodiments are particularly envisioned for substitution on a C₃_6 carbocyclyl, 4- to 6-membered heterocyclyl, or 5- to 6-membered ring.

[0087] In certain embodiments, at least one instance of R is Ci_₃haloalkyl, e.g., C haloalkyl (-CF₃, -CCI₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). Such embodiments are also particularly envisioned for substitution on a C₃_₆ carbocyclyl, 4- to 6- membered heterocyclyl, or 5- to 6-membered ring.

[0088] In certain embodiments, at least one instance of R is halogen, i.e., -F, -CI, -Br, or -I. In certain embodiments, at least one instance of R is -F or -CI. Such embodiments are also particularly envisioned for substitution on a C₃_₆ carbocyclyl, 4- to 6-membered heterocyclyl, or 5- to 6-membered ring.

[0089] In certain embodiments, at least one instance of R is -CN. For example, in certain embodiments, Ci_3 alkyl groups are contemplated substituted by -CN. In certain embodiments, C₂ alkyl groups are contemplated substituted by 1 -CN group, e.g., of formula:

[0090] In certain embodiments, at least one instance of R is -OR , wherein R is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. For example, in certain embodiments, Ci_3 alkyl groups are contemplated substituted by -OR . In certain embodiments, Ci_3 alkyl groups are contemplated substituted by 1 or 2 -OR groups, e.g., of formula:

[0091] In certain embodiments, R is -OR , wherein R is hydrogen.

[0092] In certain embodiments, R is -OR , wherein R is unsubstituted C_1-3alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH3), or unsubstituted C3 alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[0093] In certain embodiments, R^C1 is -OR^clB, wherein R^C1B is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI3, -CFCI₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, R^C1 is -OR^clB, wherein R^C1B is -CF₃, -CH₂F, -CHF₂, -CH₂C1, - CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[0094] In certain embodiments, R^C1 is -OR^clB, wherein R^C1B is C₃_6carbocylyl or 4-6 membered heterocyclyl , each independently unsubstituted or substituted with 1 or 2 R^D1 groups, wherein R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_ ₃haloalkyl, and wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl. In certain embodiments, R is -OR , wherein R is C3carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^C1 is -OR^clB, wherein R^C1B is 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^C1 is -OR^clB, wherein R^C1B is 5-membered

heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R is -OR , wherein R is 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain

, D1

embodiments, such groups are unsubstituted by R . In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH3), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). [0095] In certain embodiments, at least one instance of R is -SR , wherein R is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups.

[0096] In certain embodiments, R is -SR , wherein R is hydrogen.

[0097] In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is unsubstituted C_1-3alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH3), or unsubstituted C3 alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[0098] In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[0099] In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is C₃_6carbocylyl or 4-6 membered heterocyclyl , each independently unsubstituted or substituted with 1 or 2 R^D1 groups, wherein R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_ ₃haloalkyl, and wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl. In certain embodiments, R is -SR , wherein R is C3carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^C1 is -SR^C1B, wherein R^C1B is 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R is -SR , wherein R is 6-membered heterocyclyl (e.g.,

tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain

embodiments, such groups are unsubstituted by R^D1. In other embodiments, such groups are substituted, e.g., wherein at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00100] In certain embodiments, at least one instance of R^C1 is -N(R^C1A)(R^C1B) or - C(=0)N(R^C1A)(R^C1B) or -OC(=0)N(R^clA)(R^clB) or -NR^CIBC(=0)N(R^C1A)(R^C1B), wherein R is unsubstituted Ci_3alkyl, Ci_3haloalkyl, C3-₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; R is hydrogen, unsubstituted Ci_₃alkyl, C ^haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; or R^C1A and R^C1B are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. For example, in certain embodiments, Ci_₃ alkyl groups are contemplated substituted by -N(R )(R ) or - C(=0)N(R )(R ). In certain embodiments, Ci_₃ alkyl groups are contemplated substituted by 1 -N(R^C1A)(R^C1B) or -C(=0)N(R^C1A)(R^C1B) group, e.g., of formula:

[00101] In certain embodiments of -N(R^C1A)(R^C1B) or -C(=0)N(R^C1A)(R^C1B) or - OC(=0)N(R^clA)(R^clB) or -NR^CIBC(=0)N(R^C1A)(R^C1B), R^C1A and R^C1B do not join to form a cyclic ring structure, such that R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and R^C1B is hydrogen, unsubstituted Ci_ ₃alkyl, Ci_₃haloalkyl, C₃_6carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4- 6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R is hydrogen or unsubstituted Ci_₃alkyl (e.g., -CH ). In certain

embodiments, R is unsubstituted Ci_₃alkyl (e.g., -CH ), Ci_₃haloalkyl (e.g., -CF , -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃), C₃carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups, 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, , such groups are substituted, e.g., wherein at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00102] In certain embodiments of -N(R^C1A)(R^C1B) or -C(=0)N(R^C1A)(R^C1B) or - OC(=0)N(R^clA)(R^clB) or -NR^CIBC(=0)N(R^C1A)(R^C1B), wherein R^C1B is hydrogen or -CH₃, any recited instance of - N(R )(R ) (e.g., for example, alone or part of a group, such as - C(=0) N(R^C1A)(R^C1B) or -NR^CIBC(=0)N(R^C1A)(R^C1B) or -OC(=0)N(R^clA)(R^clB))

independently refers to a group selected from:

wherein R is as defined herein.

[00103] In certain embodiments of -N(R^C1A)(R^C1B) or -C(=0)N(R^C1A)(R^C1B) or - OC(=0)N(R^clA)(R^clB) or -NR^CIBC(=0)N(R^C1A)(R^C1B), R^C1A and R^C1B are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups, e.g., for example, in certain embodiments, R and R are joined to form an 4- membered heterocyclyl (e.g., azetidinyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5- membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, , such groups are substituted, e.g., wherein at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (i.e., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (-CH₃, -CH₂CH₃).

[00104] In certain embodiments of -N(R^C1A)(R^C1B) or -C(=0)N(R^C1A)(R^C1B) or - OC(=0)N(R^clA)(R^clB) or -NR^CIBC(=0)N(R^C1A)(R^C1B), wherein R^C1A and R^C1B are joined to form an 4- to 6- membered heterocyclyl, any recited instance of - N(R )(R ) (e.g., for example, alone or part of a group, such as -C(=0) N(R )(R ) or - NR^CIBC(=0)N(R^C1A)(R^C1B) or -OC(=0)N(R^clA)(R^clB)) independently refers to a group selected from:

wherein R is as defined herein.

p| |

[00105] In certain embodiments, any recited instance of - N(R )(R ) (e.g., for example, alone or part of a group, such as -C(=0) N(R^C1A)(R^C1B) or NR^CIBC(=0)N(R^C1A)(R^C1B) or - O =0)N(R^clA)(R^clB)) independently refers to:

[00106] In certain embodiments, at least one instance of R is -C(=0)R or - C(=0)OR^clA, wherein R^C1A is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. For example, in certain embodiments, Ci_ alkyl groups are contemplated substituted by -C(=0)R or -C(=0)OR . In certain embodiments, Ci_₃ alkyl groups are contemplated substituted by one (1) -C(=0)OR"^1A group, e.g., of formula:

[00107] In certain embodiments, at least one instance of R is -C(=0)R or - C(=0)OR^clA, wherein R^C1A is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00108] In certain embodiments, at least one instance of R is -C(=0)R or -

C(=0)OR^c , wherein R^C1A is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC "11₂2,

CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, - CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, - CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, -C(=0)R^C1A or -C(=0)OR^clA, wherein R^C1A is -CF₃, -CH₂F, -CHF₂, -CH₂

CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00109] IInn cceerrttaaiinn eemmbbooddiimi ents, at least one instance of R is -C(=0)R or -

C(=0)OR , wherein R is C₃_₆carbocylyl or 4-6 membered heterocyclyl , each independently unsubstituted or substituted with 1 or 2 R^D1 groups, wherein R^D1 is

independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, and wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl. In certain embodiments, at least one instance of R^C1 is -C(=0)R^C1A or -C(=0)OR^clA, wherein R^C1A is C₃carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is -C(=0)R^C1A or -C(=0)OR^clA, wherein R^C1A is 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R is -C(=0)R or -C(=0)OR , wherein R is 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is -C(=0)R^C1A or - C(=0)OR , wherein R is 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups a unsubstituted by R^D1. In other embodiments, , such groups are substituted, e.g., wherein at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCF or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00110] In certain embodiments, at least one instance of R is -OC(=0)R or - OC(=0)OR^clA, wherein R^C1A is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R groups.

[00111] In certain embodiments, at least one instance of R is -OC(=0)R or - OC(=0)OR^clA, wherein R^C1A is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00112] In certain embodiments, at least one instance of R is -OC(=0)R or - OC(=0)OR^clA, wherein R^C1A is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, - CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, - CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, - CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, at least one instance of R^C1 is -OC(=0)R^clA or -OC(=0)OR^clA, wherein R^C1A is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00113] In certain embodiments, at least one instance of R is -OC(=0)R or - OC(=0)OR , wherein R is C₃_₆carbocylyl or 4-6 membered heterocyclyl , each independently unsubstituted or substituted with 1 or 2 R^D1 groups, wherein R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, and wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl. In certain embodiments, at least one instance of R^C1 is -OC(=0)R^clA or -OC(=0)OR^clA, wherein R^C1A is C₃carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is -OC(=0)R^clA or -OC(=0)OR^clA, wherein R^C1A is 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is -OC(=0)R^clA or -OC(=0)OR^clA, wherein R is 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is - OC(=0)R^clA or -OC(=0)OR^clA, wherein R^C1A is 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00114] In certain embodiments, at least one instance of R is -S(0)₂R , wherein R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups.

[00115] In certain embodiments, at least one instance of R is -S(0)₂R , wherein R is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH ), unsubstituted C₂ alkyl (-CH₂CH ), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00116] In certain embodiments, at least one instance of R is -S(0)₂R , wherein R is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, - CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or - CH(CH₃)CF₃). In certain embodiments, at least one instance of R is -OC(=0)R or - OC(=0)OR^clA, wherein R^C1A is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, - CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00117] In certain embodiments, at least one instance of R is -S(0)₂R , wherein R is C₃_6carbocylyl or 4-6 membered heterocyclyl , each independently unsubstituted or substituted with 1 or 2 R^D1 groups, wherein R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, and wherein R is hydrogen, unsubstituted C

3 ¾∑alkyl, or Ci_₃haloalkyl. In certain embodiments, at least one instance of R is -S(0)₂R , wherein R^U1A is C₃carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^m groups. In certain embodiments, at least one instance of R is -S(0)₂R , wherein R is 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 is -S(0)₂R^C1A, wherein R^C1A is 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, at least one instance of R^C1 -S(0)₂R^C1A, wherein R^C1A is 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). [00118] In certain embodiments, at least one instance of R^C1 is -NR^CIBC(=0)R^C1A or - NR^CIBC(=0)OR^clA, wherein R^C1A is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; R^C1B is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; or R^C1A and R^C1B are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups.

[00119] In certain embodiments of -NR^CIBC(=0)R^C1A or -NR^CIBC(=0)OR^clA, R^C1A and

is unsubstituted Ci_₃alkyl, Ci_ ₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups, and R^C1B is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^C1B is hydrogen or unsubstituted Ci_₃alkyl (e.g., -CH₃). In certain embodiments, R is unsubstituted Ci_₃alkyl (e.g., -CH₃), Ci_₃haloalkyl (e.g., - CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃), C₃carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups, 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (i.e., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (-CH₃, -CH₂CH₃).

[00120] In certain embodiments of -NR^CIBC(=0)R^C1A or -NR^CIBC(=0)OR^clA, R^C1A and are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups, e.g., for example, in certain embodiments, R^C1A and R^C1B are joined to form an 4- membered heterocyclyl (e.g., azetidinyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5-membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In

, D1

certain embodiments, such groups are unsubstituted by R . In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). [00121] In certain embodiments wherein R and R are joined to form an 4- to 6- membered heterocyclyl, any recited instance of -NR C(=0)OR independently refers to the group:

(IV) Ring B Substituents: Groups comprising -N(R^N1A)(R^N1B), -N(R^B1A)(R^B1B), and -

_N(RB4A_{) (R}B4_B)

[00122] Various embodiments of Ring B substituents, e.g., wherein R^N1 is - C(=0)N(R^N1A)(R^N1B), R^B1 is -N(R^B1A)(R^B1B) (or comprises such a group, such as - C(=0)N(R^B1A)(R^B1B), -OC(=0)N(R^B1A)(R^B1B), or -NR^BIBC(=0)N(R^B1A)(R^B1B)), and at least one of R^B4, R^B5, R^B6, and R^B7 is -N(R^B4A)(R^B4B) (or comprises such a group, such as - C(=0)N(R^B4A)(R^B4B), -OC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)N(R^B4A)(R^B4B)), are contemplated herein.

[00123] For example, in each of the above-recited instances of -C(=0)N(R^N1A)(R^N1B), - N(R^B1A)(R^B1B), or -N(R^B4A)(R^B4B), in certain embodiments the two R groups attached to the amino (N) atom do not join to form a cyclic ring structure, such that R^N1A , R^B1A, or R^B4A is unsubstituted Ci_3alkyl, Ci_3haloalkyl, C3-₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and R^N1B, R^B1B, or R^B4B is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_ ₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered

heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, R^N1B, R^B1B, or R^B4B is hydrogen or unsubstituted Ci_₃alkyl {e.g., -CH₃). In certain

embodiments, R^N1A , R^B1A, or R^B4A is unsubstituted Ci_₃alkyl (e.g., -CH₃), Ci_₃haloalkyl (e.g., -CF₃, -CH₂F, -CHF₂, -CH₂CI, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃), C3carbocylyl (e.g., cyclopropyl) unsubstituted or substituted with 1 or 2 R^D1 groups, 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5-membered heterocyclyl (e.g., tetrahydrofuranyl), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., tetrahydropyranyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃). [00124] In certain embodiments of -C(=0)N(R^{N 1A})(R^{N 1B}), -N(R )(R ), or - N(R^B4A)(R^B4B), wherein R^N1B, R^B1B, or R^B4B is hydrogen or -CH₃, any recited instance of - N(R^N1A)(R^N1B), -N(R^B1A)(R^B1B), or -N(R^B4A)(R^B4B) (e.g., for example, alone or part of a group) independently refers to a group selected from:

for -N(R^N1A)(R^N1B);

>B4A

B4A N

I

H or CH₃ for N(R^B4A)(R^B4B).

wherein R^N1A, R^B1A, and R^B4A are as defined herein.

[00125] In certain embodiments of -C(=0)N(R^N1A)(R^N1B), -N(R^B1A)(R^B1B), or - N(R^B4A)(R^B4B), the two R groups attached to the amino (N) atom are joined to form an 4- to 6- membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups, e.g., for example, in certain embodiments, R^N1A and R^N1B (or R^B1A and R^B1B, or R^B4A and R^B4B) are joined to form an 4- membered heterocyclyl (e.g., azetidinyl), unsubstituted or substituted with 1 or 2 R^D1 groups, 5-membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), unsubstituted or substituted with 1 or 2 R^D1 groups, or 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl), unsubstituted or substituted with 1 or 2 R^D1 groups. In certain embodiments, such groups are unsubstituted by R^D1. In other embodiments, at least one instance of R^D1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^D1A (i.e., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (-CH₃, -CH₂CH₃).

[00126] In certain embodiments of -C(=0)N(R^N1A)(R^N1B), -N(R^B1A)(R^B1B), or - N(R^B4A)(R^B4B), wherein R^N1A and R^N1B, or R^B1A and R^B1B, or R^{B A} and R^B4B, are joined to form an 4- to 6- membered heterocyclyl, any recited instance of -N(R^N1A)(R^N1B), - N(R^B1A)(R^B1B), or -N(R^B4A)(R^B4B) (e.g., for example, alone or part of a group) independently refers to a group selected from:

wherein R is as defined herein. [00127] In certain embodiments, any recited instance of -C(=0)N(R^{N 1A})(R^{N 1B}), - N(R^B1A)(R^B1B), or -N(R^B4A)(R^B4B) (e.g., for example, alone or part of a group) independently refers to:

(V) Ring B Groups comprising R^N1, R⁸², and Li

[00128] Groups R^N1, R^B2 and/or L_{l s} are present in Ring B groups of formula (iii), (v), (vi), vii), (viii), (ix), (x), (xii), (xiii), (xxii (xxvii), (xxviii), and (xxiv):

(^xxii) (xxvii) (xxviii)

(xxiv).

[00129] Various embodiments of R^N1 , R^B2 and L¹ are further contemplated herein. In particular, embodiments wherein R^N1 is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A, is further contemplated herein.

[00130] Embodiments wherein R^N1 is -C(=0)N(R^N1A)(R^N1B) is contemplated in a preceding section. For example, in certain embodiments, R^N1 is -C(=0)N(R^N1A)(R^N1B), wherein R^N1A and R^N1B are as defined herein. In certain embodiments, R^N1 is -C(=0)N(R^N1A)(R^N1B), wherein

wherein R is as defined herein.

[00131] In certain embodiments, R^N1 is -C(=0)N(R^N1A)(R^N1B), wherein the group - N( ^N1A)(R^N1B) is of the formula:

[00132] In certain embodiments, R is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_ ₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, R^N1 is unsubstituted Ci_₃alkyl, i.e., C_\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^N1 is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or - CH(CH₃)₂. In certain embodiments, R^N1 is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00133] In certain embodiments, R^N1 is Ci_3haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, - CFC1₂, -CF₂CI, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, - CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, R^N1 is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00134] In certain embodiments, R^N1 is substituted or unsubstituted C₃_6carbocylyl. In certain embodiments, R^N1 is substituted or unsubstituted C₃carbocylyl (e.g., substituted or unsubstituted cyclopropyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^clA (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl

-CH₃, -CH₂CH₃).

[00135] In certain embodiments, R^N1 is substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, R^N1 is a substituted or unsubstituted 4- membered heterocyclyl (e.g., azetidinyl), substituted or unsubstituted 5-membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), or substituted or unsubstituted 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR (e.g., - OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00136] In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl. [00137] In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (- CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00138] In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, - CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, - CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, - CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, - CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, R^N1 is -C(=0)R^N1A, - C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, - CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00139] In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is substituted or unsubstituted C₃_₆carbocylyl or substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or - S(0)₂R^N1A wherein R^N1A is substituted or unsubstituted C₃carbocylyl {e.g., cyclopropyl). In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is substituted or unsubstituted 4- membered heterocyclyl {e.g., azetidinyl, oxetanyl). In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is substituted or unsubstituted 5-membered heterocyclyl {e.g., tetrahydrofuranyl). In certain embodiments, R^N1 is -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A wherein R^N1A is substituted or

unsubstituted 6-membered heterocyclyl {e.g., tetrahydropyranyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR {e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl {e.g., -CH₃, -CH₂CH₃).

[00140] For example, in certain embodiments, R^N1 is a substituted or unsubstituted 4- to 6- membered heterocyclyl comprising one oxygen ring heteroatom. In certain embodiments, R^N1 is:

[00141] In certain embodiments, R is:

(a) substituted or unsubstituted Ci_₃alkyl (-CH₃, -CH₂CH₃, -CH(CH₃)₂); Ci_₃haloalkyl (-CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂ or -CH(CH₃)CF₃);

(c) substituted or unsubstituted C₃ carbocyclyl (e.g.,

(d) -C(=0)R^N1A, -C(=0)OR^N1A, or -S(0)₂R^N1A, wherein R^N1A is -CH₃, CH₂CH₃, - CH(CH₃)₂, -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or CH(CH₃)CF₃;

(e) -C(=0)N(R^N1A)(R^N1B), as previously contemplated, e.g., wherein N(R^N1A)(R^N1B) is:

(f) substituted or unsubstituted 4- membered heterocyclyl (e.g., -

(g) substituted or unsubstituted 5- membered heterocyclyl (e.g., X

(h) substituted or unsubstituted 6-membered heterocyclyl (e.g.,

[00142] In certain embodiments, wherein Ring B is of formula:

[00143] In certain embodiments f formula iii wherein Rin B is of formula:

[00144] In certain embodiments, Rin B is of formula:

[00145] In certain embodiments of formula (v), Ring B is of formula:

[00146] In certain embodiments, Ring B is of formula:

[00147] In certain embodiments of formula vi), Ring B is of formula:

[00148] In certain embodiments, Ring B is of formula:

[00149 In certain embodiments of formula x Rin B is of formula:

[00150] In certain embodiments, Ring B is of formula:

[00151] In certain embodiments of formula xii), Ring B is of formula:

[00152] In certain embodiments, Ring B is of formula:

[00153] In certain embodiments of formula (xiii), Ring B is of formula:

[00154] In certain embodiments, Ring B is of formula:

[00155] In certain embodiments of formula (xxii), represents a single or double bond (e.g., represented by =^) to provide Ring B of formula:

[00156] In certain embodiments of formula (xxii), =^ represents a single bond. In certain embodiments of formula (xxii), == represents a single bond, and the ring fusion is in the trans configuration. In certain embodiments of formula (xxii), represents a single bond, and the ring fusion is in the cis configuration. In certain embodiments of formula (xxii), =^ represents a double bond.

G

[00157] In certain embodiments of formula (xxii), G of is -CH₂- to provide a cyclopropanated Ring B of formula:

[00158] In certain embodiments of formula (xxii), x is 1 and y is 1. In certain embodiments of formula (xxii), x is 1 and y is 2. For example, in certain embodiments of formula (xxii), Ring B is of formula:

[00159] In certain embodiments of formula xxii), Ring B is of formula:

[00160] In certain embodiments of formula xxii), Ring B is of formula:

[00161] In certain embodiments of formula (xxii), Ring B is of formula: N1

[00162] In certain embodiments of formula (xxii), Ring B is of formula:

[00163] In certain embodiments of formula xxii), Ring B is of formula:

[00164] In certain embodiments of formula (xxii), Ring B is of formula:

[00165] In certain embodiments, Ring B is of formula:

(xxvii).

[00166] In certain embodiments of formula (xxvii), q is 1, 2, or 3 and w is 1. In certain embodiments of formula (xxvii), q is 2 and w is 0 or 2. For example, in certain embodiments of formula (xxvii), Ring B is of formula:

[00168] In certain embodiments of formula (xxvii), Ring B is of formula:

[00169] In certain embodiments of formula (xxvii), Ring B is of formula:

[00170] In certain embodiments of formula (xxvii), Ring B is of formula:

[00171] In certain embodiments of formula (xxvii), Ring B is of formula:

[00172] In certain embodiments, Ring B is of formula:

(xxviii).

In certain embodiments of formula (xxviii), Ring B is of formula:

[00174] In certain embodiments, Ring B is of formula:

wherein Li is -NH-, substituted or unsubstituted C₂alkylene, substituted or unsubstituted C₂alkenylene, or substituted or unsubstituted C₂alkynylene.

[00175] In certain embodiments of formula (viii), Li is -NH-. In certain embodiments of formula (viii), Li is substituted or unsubstituted C₂alkylene. In certain embodiments of formula (viii), Li is substituted or unsubstituted C₂alkenylene. In certain embodiments of formula (viii), Li is substituted or unsubstituted C₂alkynylene. In certain embodiments of formula (viii), Li is an unsubstituted C₂alkylene, unsubstituted C₂alkenylene, or unsubstituted C₂alkynylene group. However, in certain embodiments of formula (viii), Li is a substituted C₂alkylene, substituted C₂alkenylene, or substituted C₂alkynylene group, e.g., substituted with 1 R group such as -OR {e.g., -OCH₃ . Exemplary substituted L groups include:

wherein R is as defined herein, excluding hydrogen.

[00176] Furthermore, in certain embodiments of formula (viii), q is 1, 2, or 3 and w is 1. In certain embodiments of formula (viii), q is 2 and w is 0 or 2. For example, in certain embodiments of formula (viii), Ring B is of formula:

[00177] In certain embodiments of formula (viii), Ring B is of formula:

[00178 In certain embodiments Ring B is of formula:

wherein R is hydrogen, halogen, -OR , substituted or unsubstituted Ci_₃alkyl, or Ci_ ₃haloalkyl, and wherein R^B2A i_s substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl.

[00179] In certain embodiments, R^B2 is hydrogen. In certain embodiments, R^B2 is halogen, e.g., -F, -CI, -Br, or -I. In certain embodiments, R is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an

B2

unsubstituted Ci_₃alkyl. In certain embodiments, R is unsubstituted Ci_₃alkyl, i.e., C\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -

B2

CH(CH₃)₂). In certain embodiments, R is unsubstituted Ci_₃alkyl of formula -CH₃, -

B2

CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, R is substituted C _₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

, B2

[00180] In certain embodiments, R is C^haloalkyl, e.g., C_\ haloalkyl (-CF₃, -CCI₃, CFCI2, -CF2CI, -CH₂F, -CHF₂, -CH2CI, CHCI2), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, R^B2 is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00181] In certain embodiments, R^B2 is -OR^B2A, wherein R^B2A i_s substituted or

unsubstituted Ci_₃alkyl or Ci_₃haloalkyl. In certain embodiments, R^B2A is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, R^B2A is

unsubstituted Ci_₃alkyl, i.e., C_\ alkyl (-CH ), unsubstituted C₂ alkyl (-CH₂CH ), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^B2A is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, R^B2A is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00182] In certain embodiments, R is hydrogen, -OR , -F, unsubstituted Ci_₃ alkyl, Ci_

₃haloalkyl, or Ci_₃ alkyl substituted with -OR

[00183] In certain embodiments, Rin B is of formula:

[00184] In certain embodiments of formula (vii), R is hydrogen or -CH₃CH₃. For example, in certain embodiments of formula (vii), Ring B is of formula:

[00185] In certain embodiments, Ring B is of formula:

[00186] In certain embodiments ooff ffoorrmmuullaa ((iixx)),, RR iiss hhyydrogen or -CH₃. For example, in certain embodiments of formula ix), Ring B is of formula:

[00187] In certain embodiments, Ring B is of formula:

[00188] In certain embodiments of formula (xxiv), R l^B2 iiss hhydrogen or halogen (e.g., -F, -CI,

-Br, or -I). In certain embodiments of formula xxiv), Ring B is of formula:

(VI) Ring B Groups comprising R⁸¹ and optionally R⁸²

[00189] Group R^B1, and optionally group R^B2, are present in Ring B groups of formula (i), (ii), (iv), (xi), (xxiii), and (xxvi):

(xxiii) (xxvi)

[00190] Various embodiments of R are contemplated herein. In particular, embodiments wherein R^B1 is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, -CN, -OR^B1B, - SR^B1B, substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6- membered heterocyclyl, -C(=0)R^B1A, -C(=0)OR^B1A, -S(0)₂R^B1A, -OC(=0)R^B1A, - OC(=0)N(R^B1A)(R^B1B), -OC(=0)OR^B1A, -NR^BIBC(=0)R^B1A, and -NR^BIBC(=0)OR^B1A, embodiments wherein R^B2 is hydrogen, halogen, -OR^B2A, substituted or unsubstituted Ci_ ₃alkyl, or Ci_₃haloalkyl, wherein R^B2A is substituted or unsubstituted Ci_₃alkyl or Ci_ ₃haloalkyl; or R and R are joined to form a substituted or unsubstituted 4- to 6-membered heterocyclyl, are contemplated herein.

[00191] Embodiments wherein R^B1 is -N(R^B1A)(R^B1B), -C(=0)N(R^B1A)(R^B1B), - OC(=0)N(R^B1A)(R^B1B), or -NR^BIBC(=0)N(R^B1A)(R^B1B) are contemplated in a preceding section. In certain embodiments, R^B1 is -N(R^B1A)(R^B1B), wherein R^B1A and R^B1B are as defined herein. In certain embodiments, R^B1 is -C(=0)N(R^B1A)(R^B1B), wherein R^B1A and R^B1B are as defined herein. In certain embodiments, R^B1 is -OC(=0)N(R^B1A)(R^B1B), or - NR^BIBC(=0)N(R^B1A)(R^B1B) , wherein R^B1A and R^B1B are as defined herein. For example, in certain embodiments, R^B1 is -N(R^B1A)(R^B1B), -C(=0)N(R^B1A)(R^B1B), -OC(=0)N(R^B1A)(R^B1B), or -NR^BIB =0)N(R^B1A)(R^B1B), wherein the group -N(R^B1A)(R^B1B) is of the formula:

wherein R is as defined herein.

[00192] In certain embodiments, R is -N(R )(R ), -C(=0)N(R^B1A)(R^B1B), - OC(=0)N(R^B1A)(R^B1B), or -NR^BIBC(=0)N(R^B1A)(R^B1B), wherein the group -N(R^B1A)(R^B1B) is of the formula:

[00193] In certain embodiments, R is halogen, i.e., -F, -CI, -Br, or -I.

[00194] In certain embodiments, R^B1 is -CN.

[00195] In certain embodiments, R^B1 is -OR^B1B or -SR^B1B, wherein R^B1B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C3-6 carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl. In certain embodiments, R^B1 is -OR^B1B or -SR^B1B, wherein R^B1B is substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl. In certain embodiments, R^B1 is -OR^B1B or -SR^B1B, wherein R^B1B is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, R^B1 is - OR^B1B or -SR^B1B, wherein R^B1B is unsubstituted Ci_₃alkyl, i.e., C_\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^B1 is -OR^B1B or -SR^B1B, wherein R^B1B is unsubstituted Ci_₃alkyl of formula CH₃, -CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, R^B1 is -OR^B1B or -SR^B1B, wherein R^B1B is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00196] In certain embodiments, R^B1 is substituted or unsubstituted C_1-3alkyl, i.e., a Ci_ alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, R^B1 is unsubstituted Ci_₃alkyl, i.e., Ci alkyl (-CH ), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^B1 is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or - CH(CH )₂. In certain embodiments, R^B1 is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00197] In certain embodiments, R^B1 is Ci_₃haloalkyl, e.g., C_\ haloalkyl (-CF , -CC1₃, - CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

embodiments, R^B1 is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃. [00198] In certain embodiments, R is substituted or unsubstituted C3_₆carbocylyl. In certain embodiments, R^B1 is substituted or unsubstituted C₃carbocylyl (e.g., substituted or unsubstituted cyclopropyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^clA (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl

-CH₃, -CH₂CH₃).

[00199] In certain embodiments, R^B1 is substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, R^B1 is a substituted or unsubstituted 4- membered heterocyclyl (e.g., azetidinyl), substituted or unsubstituted 5-membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), or substituted or unsubstituted 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR (e.g., - OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00200] In certain embodiments, R^B1 is -C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A, wherein R^B1A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl, and R^B1B is as defined herein.

[00201] In certain embodiments, R^B1 is -C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH ), unsubstituted C₂ alkyl (-CH₂CH ), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00202] In certain embodiments, R^B1 is C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is Ci_ ₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, - CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or - CH(CH₃)CF₃). In certain embodiments, R^B1 is C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is - CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00203] In certain embodiments, R^B1 is C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is substituted or unsubstituted C3_₆carbocylyl or substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, R^B1 C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, - OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is substituted or unsubstituted C₃carbocylyl (e.g., cyclopropyl). In certain embodiments, R^B1 is C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, -OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, - NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is substituted or unsubstituted 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl). In certain embodiments, R^B1 is C(=0)R^B1A, - C(=0)OR^B1A, -OC(=0)R^B1A, -OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or - S(0)₂R^B1A wherein R^B1A is substituted or unsubstituted 5-membered heterocyclyl (e.g., tetrahydrofuranyl). In certain embodiments, R^B1 is C(=0)R^B1A, -C(=0)OR^B1A, -OC(=0)R^B1A, -OC(=0)OR^B1A, -NR^B1BC(=0)R^B1A, -NR^B1BC(=0)OR^B1A, or -S(0)₂R^B1A wherein R^B1A is substituted or unsubstituted 6-membered heterocyclyl (e.g., tetrahydropyranyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^clA (e.g., -OH, -OCH3), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00204] In certain embodiments, R^B1 is unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, Ci_₃ alkyl substituted with -OR^clB, Ci_₃ alkyl substituted with -N(R^C1A)(R^C1B), Ci_₃alkyl substituted with -CN, Ci_₃ alkyl substituted with -C(=0)N(R^C1A)(R^C1B), Ci_₃ alkyl substituted with - C(=0)OR^clA, -C(=0)N(R^B1A)(R^B1B), -OC(=0)OR^B1A, -N(R^B1A)(R^B1B), -OR^B1B, -SR^B1B, - S(0)₂R^B1A, -F, -CI, -CN, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl.

[00205] In certain embodiments, R^B1 is:

(a) unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃, -CH₂CH₂CH₃, -CH(CH₃)₂); or

(b) Ci_₃haloalkyl (e.g., -CF₃, -CCI₃, -CFCI₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, - CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, - CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, -CH(CH₃)CF₃); or

(c) substituted C _₃ alkyl, e.g.,

(d) -N(R )(R ), as previously contemplated, e.g.,

(e) substitut

[00206] Furthermore, as generally defined herein, in certain embodiments, R is hydrogen, halogen, -OR , substituted or unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, wherein R^B2A is substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl.

[00207] In certain embodiments, R^B2 is hydrogen. In certain embodiments, R^B2 is halogen, e.g., -F, -CI, -Br, or -I. In certain embodiments, R is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an

B2

CH(CH )₂). In certain embodiments, R is unsubstituted Ci_₃alkyl of formula -CH , -

B2

wherein R and R are as defined herein. [00208] In certain embodiments, R is Ci_3haloalkyl, e.g., C_\ haloalkyl (-CF₃, -CCI₃, - CFC1₂, -CF2CI, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain

[00209] In certain embodiments, R^B2 is -OR^B2A, wherein R^B2A i_s substituted or

unsubstituted Ci_3alkyl or Ci_3haloalkyl. In certain embodiments, R^B2A is substituted or unsubstituted Ci_3alkyl, i.e., a Ci_3alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_3alkyl. In certain embodiments, R^B2A is

unsubstituted Ci_3alkyl, i.e., C_\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, R^B2A is unsubstituted Ci_3alkyl of formula -CH₃, -CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, R^B2A is substituted Ci_3alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00210] In certain embodiments, R is hydrogen, -OR , -F, unsubstituted Ci_3 alkyl, Ci_

3haloalkyl, or Ci_3 alkyl substituted with -OR¹ CIB

[00211] In certain embodiments, wherein R and R , ^aB^z2 are each present on Ring B, such as

, B2

Ring B of formula (i), (ii), or (xxvi), various combinations of R^B1 and R^az are contemplated herein. For example, in certain embodiments, the following R^B1 and R^B2 combinations are specifically contemplated:

a. R^B1 is -N(R^B1A)(R^B1B), -OR^B1B, -SR^B1B, -S(0)₂R^B1A, -F, -CI, -CN, - OC(=0)OR^B1A, -C(=0)N(R^B1A)(R^B1B), and R^B2 is hydrogen; or b. R^B1 is -F and R^B2 is -F; or c. R is -OR , -C(=0)N(R^B1A)(R^B1B), -CN , or Ci_₃ alkyl substituted with - OR^clB, Ci_₃ alkyl substituted with -N(R^C1A)(R^C1B), and R^B2 is substituted or unsubstituted Ci_₃ alkyl or Ci_₃haloalkyl; or

d. R^B1 is -OR^B1B and R^B2 is -OR^B2A, and each instance of R^B1B and R^B2A is

independently substituted or unsubstituted Ci_₃ alkyl or Ci_₃haloalkyl.

[00212] Furthermore, as generally defined herein, in certain embodiments, R^B1 and R^B2 are joined to form a substituted or unsubstituted 4- to 6-membered heterocyclyl, e.g., a substituted or unsubstituted 4-membered heterocyclyl, a substituted or unsubstituted 5- membered heterocyclyl, or a substituted or unsubstituted 6-membered heterocyclyl. For example, in certain embodiments, wherein R^B1 is -OR^B1B and R^B2 is -OR^B2A, R^B1B and R^B2A are joined to form a substituted or unsubstituted 5- membered heterocyclyl {e.g., dioxolanyl) or substituted or unsubstituted 6- membered heterocyclyl {e.g., dioxanyl).

[00213] In certain embodiments, Rin B is of formula:

[00214] In certain embodiments of formula (i), Ring B is of formula:

[00215 In certain embodiments of formula (i), Ring B is of formula:

[00216] In certain embodiments, Ring B is of formula:

[00217] In certain embodiments of formula (ii), Ring B is of formula:

[00218] In certain embodiments of for Ring B is of formula:

[00219] In certain embodiments, Ring B is of formula:

[00220] In certain embodiments of formula (iv), Ring B is of formula:

[00221] In certain embodiments, Rin B is of formula:

[00222] In certain embodiments of formula (xi), Ring B is of formula:

[00223] In certain embodiments, Ring B is of formula:

(xxiii).

[00224] In certain embodiments of formula (xxiii), =^ represents a single bond. In certain embodiments of formula (xxiii), represents a single bond, and the ring fusion is in the trans configuration. In certain embodiments of formula (xxiii), represents a single bond, and the ring fusion is in the cis configuration. In certain embodiments of formula (xxiii), =^ represents a double bond.

[00225] In certain embodiments Ring B is of formula:

[00226] In certain embodiments, Ring B is of formula:

[00227 In certain embodiments Ring B is of formula:

[00228] In certain embodiments of formula (xxiii), Ring B is of formula:

[00229] In certain embodiments, Ring B is of formula:

[00230] In certain embodiments of formula (xxvii), q is 1, 2, or 3 and w is 1. In certain embodiments of formula (xxvii), q is 2 and w is 0 or 2. For example, in certain embodiments of formula (xxvii), Ring B is of formula:

[00231 In certain embodiments of formula (xxvii), Ring B is of formula:

[00232] In certain embodiments of formula (xxvii), Ring B is of formula:

[00233] In certain embodiments of formula (xxvii), Ring B is of formula:

[00234] In certain embodiments of formula xxvii Rin B is of formula:

(VII) Ring B: Groups comprising R^N2, R⁸³, R⁸⁴, R⁸⁵, R⁸⁶, and/or R⁸⁷

[00235] Groups R^N2, R^B3, R^B4, R^B5, R^B6, and/or R^B7are provided in Rin formula (xiv), (xv), (xvi), (xvii), (xviii), (xix), (xx), and (xxi):

(xv) (xvi)

(xxi)

[00236] Various embodiments of R^B4, R^B5, R^B6, and R^B7are contemplated herein. In particular, embodiments wherein at least one of R^B4, R^B5, R^B6, and R^B7 is hydrogen, substituted or unsubstituted Ci_3alkyl, Ci_3haloalkyl, halogen, -CN, -OR^B4B, -SR^B4B, substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^B4A, -C(=0)OR^B4A, -S(0)₂R^B4A, -OC(=0)R^B4A, - OC(=0)N(R^B4A)(R^B4B), -OC(=0)OR^B4A, -NR^B4BC(=0)R^B4A, and -NR^B4BC(=0)OR^B4A.

[00237] Embodiments wherein R^B4, R^B5, R^B6, or R^B7 is -N(R^B4A)(R^B4B), - C(=0)N(R^B4A)(R^B4B), -OC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)N(R^B4A)(R^B4B) are contemplated in a preceding section. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -N(R^B4A)(R^B4B), wherein R^B4A and R^B4B are as defined herein. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)N(R^B4A)(R^B4B), wherein R^B4A and R^B4B are as defined herein. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is-OC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)N(R^B4A)(R^B4B) , wherein R^{B A} and R^B4B are as defined herein. For example, in certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -N(R^B4A)(R^B4B), -C(=0)N(R^B4A)(R^B4B), -OC(=0)N(R^B4A)(R^B4B), or - NR^B4BC(=0)N(R^{B4A B4B}), wherein the group -N(R^B4A)(R^B4B) is of the formula:

wherein R is as defined herein.

[00238] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -N(R^B4A)(R^B4B), C(=0)N(R^B4A)(R^B4B), -OC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)N(R^B4A)(R^B4B), wherein the

[00239] In certain embodiments, at least one of R , R , R , and R is hydrogen. In certain embodiments, two of R^B4, R^B5, R^B6, and R^B7 are hydrogen. In certain embodiments, each of R^B4, R^B5, R^B6, and R^B7 is hydrogen.

[00240] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is halogen, i.e., -F, - CI, -Br, or -I. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -CN.

[00241] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -OR^B4B or - SR^B4B, wherein R^B4B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃-6 carbocyclyl, or substituted or unsubstituted 4- to 6- membered heterocyclyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is - OR^B4B or -SR^B4B, wherein R^B4B is substituted or unsubstituted Ci_₃alkyl or C^haloalkyl In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -OR^B4B or -SR^B4B, wherein R^B4B is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R^C1 groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -OR^B4B or -SR^B4B, wherein R^B4B is unsubstituted Ci_ ₃alkyl, i.e., C_\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (- CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -OR^B4B or -SR^B4B, wherein R^B4B is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or - CH(CH₃)₂. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -OR^B4B or - SR^B4B, wherein R^B4B is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00242] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is substituted or unsubstituted Ci_₃alkyl, i.e., a Ci_₃alkyl substituted by 1, 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is unsubstituted Ci_₃alkyl, i.e., C\ alkyl (-CH₃), unsubstituted C₂ alkyl (- CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is substituted Ci_ ₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00243] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CCI₃, -CFCI₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00244] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is substituted or unsubstituted C₃_₆carbocylyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is substituted or unsubstituted C₃carbocylyl (e.g., substituted or unsubstituted cyclopropyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^clA (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00245] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is a substituted or unsubstituted 4- membered heterocyclyl (e.g., azetidinyl), substituted or unsubstituted 5-membered heterocyclyl (e.g., pyrrolidinyl, pyrrolidin-2-one, oxazolidin-2-one), or substituted or unsubstituted 6-membered heterocyclyl (e.g., morpholinyl, piperidinyl, piperazinyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R^C1 is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR^clA (e.g., -OH, -OCH₃), or unsubstituted d_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00246] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, - C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, -NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or - S(0)₂R^B4A, wherein R^B4A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl, and R^B4B is as defined herein.

[00247] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, - C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, -NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or - S(0)₂R^B4A, wherein R^B4A is unsubstituted Ci_₃alkyl, i.e., unsubstituted Ci alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂).

[00248] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, - C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, -NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or - S(0)₂R^B4A, wherein R^B4A is Ci_₃haloalkyl, e.g., Ci haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, - CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (-CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, - CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, - CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, -C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or -S(0)₂R^B4A, wherein R^{B A} is -CF₃, -CH₂F, - CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00249] In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, - C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, -NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or - S(0)₂R^B4A, wherein R^B4A is substituted or unsubstituted C3_₆carbocylyl or substituted or unsubstituted 4-6 membered heterocyclyl. In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, -C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or -S(0)₂R^B4A, wherein R^{B A} is substituted or unsubstituted C₃carbocylyl (e.g., cyclopropyl). In certain embodiments, at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, -C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or -S(0)₂R^B4A, wherein R^{B A} is substituted or unsubstituted 4- membered heterocyclyl (e.g., azetidinyl, oxetanyl). at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, -C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or -S(0)₂R^B4A, wherein R^{B A} is substituted or unsubstituted 5-membered heterocyclyl (e.g., tetrahydrofuranyl). at least one of R^B4, R^B5, R^B6, and R^B7 is -C(=0)R^B4A, -C(=0)OR^B4A, -OC(=0)R^B4A, -OC(=0)OR^B4A, - NR^B4BC(=0)R^B4A, -NR^B4BC(=0)OR^B4A, or -S(0)₂R^B4A, wherein R^{B A} is substituted or unsubstituted 6-membered heterocyclyl (e.g., tetrahydropyranyl). In certain embodiments, such groups are unsubstituted by R . In other embodiments, such groups are substituted, e.g., wherein at least one instance of R is halogen (i.e., -F, -CI, -Br, or -I), -CN, -OR (e.g., -OH, -OCH₃), or unsubstituted Ci_₃alkyl (e.g., -CH₃, -CH₂CH₃).

[00250] In certain embodiments, at least one instance of R^B4, R^B5, R^B6, and R^B7, is Ci_₃ alkyl, Ci_₃haloalkyl, Ci_₃ alkyl substituted with -OR , Ci_₃ alkyl substituted with - N(R^C1A)(R^C1B), Ci_₃alkyl substituted with -CN, Ci_₃ alkyl substituted with - C(=0)N(R^C1A)(R^C1B), Ci_₃ alkyl substituted with -C(=0)OR^clA, -C(=0)N(R^B4A)(R^B4B), - OC(=0)OR^B1A, -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, -S(0)₂R^B4A, -F, -CI, -CN, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl.

[00251] Various combination s of the above described embodiemnts of R^B4, R^B5, R^B6, and R^B7 is further contemplated herein.

[00252] For example, in certain embodiments, wherein Ring B is of formula (xiv), various combinations of R^B5 and R^B7 are contemplated, e.g., wherein:

a. each instance of R^B5 and R^B7 is hydrogen; or

b. each instance of R^B5 and R^B7 is independently -OR^B4B; or

c. R^B5 is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl; and R^B7 is hydrogen; or d. R^B5 is hydrogen and R^B7 is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, or substituted or unsubstituted C₃ carbocyclyl.

[00253] In certain embodiments, wherein Ring B is of formula (xv), various combinations of R^B5, R^B6, and R^B7 are contemplated, e.g., wherein:

a. each instance of R^B5, R^B6, and R^B7 is hydrogen; or

b. R^B5is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B6 and R^B7 are hydrogen; or

c. R^B6is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B5 and R^B7 are hydrogen; or

d. R^B7is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B5 and R^B6 are hydrogen.

[00254] In certain embodiments, wherein Ring B is of formula (xvi), various combinations of R^B4, R^B6 and R^B7 are contemplated, e.g., wherein:

a. R^B4is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted

Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or ssuubbssttiittuutteedd oorr unsubstituted 4-6 membered heterocyclyl, and R and R are hydrogen; or

b. RR^BB6biiss --NN((RR^B4' )(R ), -OR , -SR. , halogen, substituted or unsubstituted

c. RR^B7iiss --NN((RR^B4' )(R ), -OR , -SR. , halogen, substituted or unsubstituted

d. RR^BB44aanndd RR^BB66aarr,e-N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or

unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B7 is hydrogen.

[00255] In certain embodiments, wherein Ring B is of formula (xvii), various combinations of R^B5 and R^B6 are contemplated, e.g., wherein:

a. R^B5is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_3 alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B6 is hydrogen; or

b. R^B6is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B5 is hydrogen.

[00256] Furthermore, as generally defined herein, in certain embodiments, each instance of R is independently hydrogen, unsubstituted Ci_₃alkyl, or C^haloalkyl, provided at least one instance of R^B3 is hydrogen. In certain embodiments, each instance of R^B3 is hydrogen. In

B3

certain embodiments, one instance of R is unsubstituted Ci_₃alkyl (e.g., -CH₃) or Ci_

3haloalkyl (e.g., -CF₃). R^B3 is hydrogen or -CH₃, provided at least one instance of R^B3 is hydrogen.

[00257] Furthermore, as generally defined herein, in certain embodiments, each instance of R^N2 and R^B8 is independently substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl, or R^N2

B8

and R are joined to form a substituted or unsubstituted 5- to 6-membered ring.

[00258] In certain embodiments, each instance of R^N2 and R^B8 is independently substituted or unsubstituted Ci_₃alkyl or C^haloalkyl.

[00259] In certain embodiments, at least one of R^N2 and R^B8 is substituted or unsubstituted Ci_₃alkyl, i.e. , a Ci_₃alkyl substituted by 1 , 2, or 3 R groups as previously described herein, or an unsubstituted Ci_₃alkyl. In certain embodiments, at least one of R^N2 and R^B8 is unsubstituted Ci_₃alkyl, i.e., C_\ alkyl (-CH₃), unsubstituted C₂ alkyl (-CH₂CH₃), or unsubstituted C₃ alkyl (-CH₂CH₂CH₃ or -CH(CH₃)₂). In certain embodiments, at least one of R^N2 and R^B8 is unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or -CH(CH₃)₂. In certain embodiments, at least one of R^N2 and R^B8 is substituted Ci_₃alkyl, e.g., of formula:

wherein R and R are as defined herein.

[00260] In certain embodiments, at least one of R^N2 and R^B8 is Ci_₃haloalkyl, e.g., C_\ haloalkyl (-CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂), C₂ haloalkyl (- CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1), or C₃ haloalkyl (-CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃). In certain embodiments, at least one of R^N2 and R^B8 is -CF₃, -CH₂F, -CHF₂, -CH₂C1, -CH₂CF₃, -CH₂CHF₂, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

[00261] Alternatively, in certain embodiments, R^N2 and R^B8 are joined to form a substituted or unsubstituted 5- to 6-membered ring. In certain embodiments, R^N2 and R^B8 are joined to form a substituted or unsubstituted 5-membered ring. In certain embodiments, R^N2 and R^B8 are joined to form a substituted or unsubstituted 6-membered ring. In certain embodiments, R^N2 and R^B8 are joined to form an unsubstituted ring.

[00262] In certain embodiments, each instance of R^B8 and R^N2 is independently -CH₃, - CH₂CH₃, -CH₂CH₂CH₃, -CH(CH₃)₂, -CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, -CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, - CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃; or R^N2 and R^B8 are joined to form an unsubstituted 5-membered ring.

[00263] In certain embodiments, Rin B is of formula:

[00264] In certain embodiments of formula (xiv), Ring B is of formula:

73

[00269] In certain embodiments, Ring B is of formula:

[00270] In certain embodiments of formula (xvii), Ring B is of formula:

[00271] In certain embodiments, Ring B is of formula:

(xviii).

[00272] In certain embodiments of formula (xviii), Ring B is of formula:

[00273] In certain embodiments Ring B is of formula:

In certain embodiments of formula xix), Ring B is of formula:

[00275] In certain embodiments Ring B is of formula:

[00276] In certain embodiments of formula xx), Ring B is of formula:

[00277] In certain embodiments, Ring B is of formula:

[00278] In certain embodiments of formula xxi), Ring B is of formula:

(VIII) Other Ring B groups

[00279] Other Ring B groups contemplated herein include Ring B groups of formula (xxv) and (xxix). For example, in certain ing B is of formula:

(xxv).

[00280] In certain embodiments, Ring B is of formula:

wherein R¹, R^2a, R^2b, R³, Ring A, and = are as defined herein.

G

[00281] In certain embodiments, represents a double or single bond (e.g., represented by =^) to provide a Ring B of formula:

[00282] In certain embodiments of formula (xxix), =^ represents a single bond. In certain embodiments of formula (xxix), represents a single bond, and the ring fusion is in the trans configuration. In certain embodiments of formula (xxix), represents a single bond, and the ring fusion is in the cis configuration. In certain embodiments of formula (xxix), represents a double bond.

G

[00283] In certain embodiments of formula (xxix), G of is -CH₂- to provide a cyclopropanated Ring B of formula:

[00284] In certain embodiments of formula (xxix), Ring B is of formula:

Various Contemplated Combinations of Specific Embodiments [00285] Various combinations of specific embodiments as described herein are specifically contemplated.

[00286] For example, in certain embodiments, wherein Ring B is of formula (i), and wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00287] In certain embodiments, Ring B is of formula (i), wherein Ring A is of formula (A- ii), and wherein R^A5 is -CH₃, R^A4 is -Br, and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00288] In certain embodiments, Ring B is of formula (ii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00289] In certain embodiments, Ring B is of formula (iii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00290] In certain embodiments, Ring B is of formula (iv), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00291] In certain embodiments, Ring B is of formula (v), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00292] In certain embodiments, Ring B is of formula (vi), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00293] In certain embodiments, Ring B is of formula (vii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00294] In certain embodiments, Ring B is of formula (viii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00295] In certain embodiments, Ring B is of formula (viii), wherein Ring A is of formula (A-iii), and wherein each of R^ and R^A5 is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00296] In certain embodiments, Ring B is of formula (ix), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00297] In certain embodiments, Ring B is of formula (ix), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00298] In certain embodiments, Ring B is of formula (x), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00299] In certain embodiments, Ring B is of formula (x), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00300] In certain embodiments, Ring B is of formula (xi), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00301] In certain embodiments, Ring B is of formula (xii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00302] In certain embodiments, Ring B is of formula (xiii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00303] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00304] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-i), and wherein R^A1 is -CH₃ and R^ is -CH₂CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00305] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -CI, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00306] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -Br, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00307] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -CN, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00308] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00309] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is hydrogen, and R^A5 is -CH₂CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00310] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is hydrogen, and R^A5 is -CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00311] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -Br, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00312] In certain embodiments, Ring B is of formula (xiv), wherein Ring A is of formula (A-ii), and wherein R^ is -CN, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00313] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

harmaceutically acceptable salt thereof. [00314] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-i), and wherein R^A1 is -CH₃ and R^ is -CH₂CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00315] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-i), and wherein R^A1 is -CH₂CH and R^ is -CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00316] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -Br, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00317] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -CI, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00318] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -Br, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00319] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -I, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00320] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00321] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CN, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00322] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00323] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is -hydrogen, and R^A5 is -CH₂CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00324] In certain embodiments, Ring B is of formula (xv), wherein Ring A is of formula (A-ii), and wherein R^ is -CH , R^A4 is -hydrogen, and R^A5 is -CH₂CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00325] In certain embodiments, Ring B is of formula (xvi), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. [00326] In certain embodiments, Ring B is of formula (xvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00327] In certain embodiments, Ring B is of formula (xviii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00328] In certain embodiments, Ring B is of formula (xix), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00329] In certain embodiments, Ring B is of formula (xx), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00330] In certain embodiments, Ring B is of formula (xxi), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00331] In certain embodiments, Ring B is of formula (xxii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00332] In certain embodiments, Ring B is of formula (xxii), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00333] In certain embodiments, Ring B is of formula (xxii), wherein Ring A is of formula (A-ii), and wherein R^ is -Br, R^A4 is -hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00334] In certain embodiments, Ring B is of formula (xxiii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00335] In certain embodiments, Ring B is of formula (xxiv), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00336] In certain embodiments, Ring B is of formula (xxv), wherein Ring A is of formula (A-i), and wherein eac ^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00337] In certain embodiments, Ring B is of formula (xxvi), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃ provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00338] In certain embodiments, Ring B is of formula (xxvi), wherein Ring A is of formula (A-i), and wherein R^A1 is -CH₃ and nd R^ is -CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00339] In certain embodiments, Ring B is of formula (xxvi), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -CI, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00340] In certain embodiments, Ring B is of formula (xxvi), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -Br, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00341] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00342] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-i), and wherein R^A1 is -CH₃ and R^ is -CH₂CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00343] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CH , R^A4 is -CI, and R^A5 is -CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00344] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00345] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is hydrogen, and R^A5 is -CH₂CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00346] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is hydrogen, and R^A5 is -CH₂CH , provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00347] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CI, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00348] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -Br, R^A4 is hydrogen, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00349] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -Br, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00350] In certain embodiments, Ring B is of formula (xxvii), wherein Ring A is of formula (A-ii), and wherein R^ is -CH₃, R^A4 is -CN, and R^A5 is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00351] In certain embodiments, Ring B is of formula (xxviii), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof.

[00352] In certain embodiments, Ring B is of formula (xxix), wherein Ring A is of formula (A-i), and wherein each of R^A1 and R^ is -CH₃, provided is a compound of formula:

or a pharmaceutically acceptable salt thereof. (X) Exemplary Compounds

[00353] In certain embodiments, a compound of Formula (I) is selected from any one of the compounds provided in Table 1 , and pharmaceutically acceptable salts thereof.

[00354] In certain embodiments, the compound of Formula (I) is not a compound or pharmaceutically acceptable salt thereof as disclosed in PCT/US2014/028463, the disclosure of which is incorporated herein by reference.

[00355] In certain embodiments, compounds of Formula (I), wherein R^A1 and R^ are each -CH₃, i.e., to provide a Ring A of formu

are specifically excluded.

[00356] In certain embodiments, compounds of Formula (I), wherein R^2a, R^2b, and R³ are any of the following specific combinations:

a. R^2a is hydrogen, R^2b is hydrogen, and R³ is -CH₃; and/or

b. R^2a is hydrogen, R^2b is hydrogen, and R³ is -F; and/or

c. R^2a is hydrogen, R^2b is hydrogen, and R³ is -CI; and/or

d. R^2a is -CI, R^2b is hydrogen, and R³ is -CI; and/or

e. R^2a is -CI, R^2b is hydrogen, and R³ is -F; and/or

f. R^2a is -F, R^2b is hydrogen, and R³ is -CI; and/or

g. R^2a is -CI, R^2b is hydrogen, and R³ is -CH₃; and/or

h. R^2a is -F, R^2b is hydrogen, and R³ is -CH₃; and/or

i. R^2a is -CF₃, R^2b is hydrogen, and R³ is -CH₃; and/or

j . R^2a is -CH₃, R^2b is hydrogen, and R³ is -CH₃; and/or k. R^2a is hydrogen, R^2b is -CI, and R³ is -CH₃;

are specifically excluded.

[00357] In certain embodiments, any one or all of the below compounds, and pharmaceutically acceptable salts thereof, are specifically excluded:

ı77

181

ı83

ı84

ı85

ı86

191

ı92

ı94

240

242

Ĳ43

Ĳ44











250

251

Ĳ54

Ĳ55







260

262

Ĳ63

Ĳ64

Ĳ65

Ĳ66







270

271

272

Ĳ74

Ĳ75

Ĳ76





280

281

282

Ĳ84

Ĳ85

Ĳ87

Ĳ88

290

291

292

Ĳ93

Ĳ94

Ĳ95

Ĳ96

Ĳ97

Ĳ99

300

301

302

303

310

313

T/US2015/050712

353

ĳ54

ĳ55

and pharmaceutically acceptable salts thereof.

[00358] In certain embodiments, a provided compound inhibits CARMl . In certain embodiments, a provided compound inhibits wild-type CARMl . In certain embodiments, a provided compound inhibits a mutant CARMl . In certain embodiments, a provided compound inhibits CARMl, e.g., as measured in an assay described herein. In certain embodiments, the CARMl is from a human. In certain embodiments, a provided compound inhibits CARMl at an IC₅₀ less than or equal to 10 μΜ. In certain embodiments, a provided compound inhibits CARMl at an IC₅₀ less than or equal to 1 μΜ. In certain embodiments, a provided compound inhibits CARMl at an IC₅₀ less than or equal to 0.1 μΜ. In certain embodiments, a provided compound inhibits CARMl in a cell at an EC₅₀ less than or equal to 10 μΜ. In certain embodiments, a provided compound inhibits CARMl in a cell at an EC₅₀ less than or equal to 1 μΜ. In certain embodiments, a provided compound inhibits CARMl in a cell at an EC₅₀ less than or equal to 0.1 μΜ. In certain embodiments, a provided compound inhibits cell proliferation at an EC₅₀ less than or equal to 10 μΜ. In certain embodiments, a provided compound inhibits cell proliferation at an EC₅₀ less than or equal to 1 μΜ. In certain embodiments, a provided compound inhibits cell proliferation at an EC₅₀ less than or equal to 0.1 μΜ. In some embodiments, a provided compound is selective for CARMl over other methyltransferases. In certain embodiments, a provided compound is at least about 10-fold selective, at least about 20-fold selective, at least about 30-fold selective, at least about 40-fold selective, at least about 50-fold selective, at least about 60-fold selective, at least about 70-fold selective, at least about 80-fold selective, at least about 90- fold selective, or at least about 100-fold selective for PRMTl relative to one or more other methyltransferases .

[00359] It will be understood by one of ordinary skill in the art that the CARMl can be wild-type CARMl, or any mutant or variant of CARMl .

[00360] The present disclosure provides pharmaceutical compositions comprising a compound described herein, e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof, as described herein, and optionally a pharmaceutically acceptable excipient. It will be understood by one of ordinary skill in the art that the compounds described herein, or salts thereof, may be present in various forms, such as amorphous, hydrates, solvates, or polymorphs. In certain embodiments, a provided composition comprises two or more compounds described herein. In certain embodiments, a compound described herein, or a pharmaceutically acceptable salt thereof, is provided in an effective amount in the pharmaceutical composition. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is an amount effective for inhibiting CARM1. In certain embodiments, the effective amount is an amount effective for treating a CARM1 -mediated disorder. In certain embodiments, the effective amount is a prophylactically effective amount. In certain embodiments, the effective amount is an amount effective to prevent a CARM1 -mediated disorder.

[00361] Pharmaceutically acceptable excipients include any and all solvents, diluents, or other liquid vehicles, dispersions, suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants, and the like, as suited to the particular dosage form desired. General considerations in formulation and/or manufacture of pharmaceutical compositions agents can be found, for example, in

Remington 's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980), and Remington: The Science and Practice of Pharmacy, 21st Edition (Lippincott Williams & Wilkins, 2005).

[00362] Pharmaceutical compositions described herein can be prepared by any method known in the art of pharmacology. In general, such preparatory methods include the steps of bringing a compound described herein (the "active ingredient") into association with a carrier and/or one or more other accessory ingredients, and then, if necessary and/or desirable, shaping and/or packaging the product into a desired single- or multi-dose unit.

[00363] Pharmaceutical compositions can be prepared, packaged, and/or sold in bulk, as a single unit dose, and/or as a plurality of single unit doses. As used herein, a "unit dose" is discrete amount of the pharmaceutical composition comprising a predetermined amount of the active ingredient. The amount of the active ingredient is generally equal to the dosage of the active ingredient which would be administered to a subject and/or a convenient fraction of such a dosage such as, for example, one-half or one-third of such a dosage.

[00364] Relative amounts of the active ingredient, the pharmaceutically acceptable excipient, and/or any additional ingredients in a pharmaceutical composition of the present disclosure will vary, depending upon the identity, size, and/or condition of the subject treated and further depending upon the route by which the composition is to be administered. By way of example, the composition may comprise between 0.1% and 100% (w/w) active ingredient.

[00365] Pharmaceutically acceptable excipients used in the manufacture of provided pharmaceutical compositions include inert diluents, dispersing and/or granulating agents, surface active agents and/or emulsifiers, disintegrating agents, binding agents, preservatives, buffering agents, lubricating agents, and/or oils. Excipients such as cocoa butter and suppository waxes, coloring agents, coating agents, sweetening, flavoring, and perfuming agents may also be present in the composition.

[00366] Exemplary diluents include calcium carbonate, sodium carbonate, calcium phosphate, dicalcium phosphate, calcium sulfate, calcium hydrogen phosphate, sodium phosphate lactose, sucrose, cellulose, micro crystalline cellulose, kaolin, mannitol, sorbitol, inositol, sodium chloride, dry starch, cornstarch, powdered sugar, and mixtures thereof.

[00367] Exemplary granulating and/or dispersing agents include potato starch, corn starch, tapioca starch, sodium starch glycolate, clays, alginic acid, guar gum, citrus pulp, agar, bentonite, cellulose and wood products, natural sponge, cation-exchange resins, calcium carbonate, silicates, sodium carbonate, cross-linked poly(vinyl-pyrrolidone) (crospovidone), sodium carboxymethyl starch (sodium starch glycolate), carboxymethyl cellulose, cross- linked sodium carboxymethyl cellulose (croscarmellose), methylcellulose, pregelatinized starch (starch 1500), microcrystalline starch, water insoluble starch, calcium carboxymethyl cellulose, magnesium aluminum silicate (Veegum), sodium lauryl sulfate, quaternary ammonium compounds, and mixtures thereof.

[00368] Exemplary surface active agents and/or emulsifiers include natural emulsifiers (e.g., acacia, agar, alginic acid, sodium alginate, tragacanth, chondrux, cholesterol, xanthan, pectin, gelatin, egg yolk, casein, wool fat, cholesterol, wax, and lecithin), colloidal clays (e.g., bentonite (aluminum silicate) and Veegum (magnesium aluminum silicate)), long chain amino acid derivatives, high molecular weight alcohols (e.g., stearyl alcohol, cetyl alcohol, oleyl alcohol, triacetin monostearate, ethylene glycol distearate, glyceryl monostearate, and propylene glycol monostearate, polyvinyl alcohol), carbomers (e.g., carboxy polymethylene, polyacrylic acid, acrylic acid polymer, and carboxyvinyl polymer), carrageenan, cellulosic derivatives (e.g., carboxymethylcellulose sodium, powdered cellulose, hydroxymethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose, methylcellulose), sorbitan fatty acid esters (e.g., polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene sorbitan (Tween 60), polyoxyethylene sorbitan monooleate (Tween 80), sorbitan monopalmitate (Span 40), sorbitan monostearate (Span 60], sorbitan tristearate (Span 65), glyceryl monooleate, sorbitan monooleate (Span 80)), polyoxyethylene esters (e.g., polyoxyethylene monostearate (Myrj 45), polyoxyethylene hydrogenated castor oil, polyethoxylated castor oil, polyoxymethylene stearate, and Solutol), sucrose fatty acid esters, polyethylene glycol fatty acid esters (e.g., Cremophor™), polyoxyethylene ethers, (e.g., polyoxyethylene lauryl ether (Brij 30)), poly(vinyl-pyrrolidone), diethylene glycol monolaurate, triethanolamine oleate, sodium oleate, potassium oleate, ethyl oleate, oleic acid, ethyl laurate, sodium lauryl sulfate, Pluronic F68, Poloxamer 188, cetrimonium bromide, cetylpyridinium chloride, benzalkonium chloride, docusate sodium, and/or mixtures thereof.

[00369] Exemplary binding agents include starch (e.g., cornstarch and starch paste), gelatin, sugars (e.g., sucrose, glucose, dextrose, dextrin, molasses, lactose, lactitol, mannitol, etc.), natural and synthetic gums (e.g., acacia, sodium alginate, extract of Irish moss, panwar gum, ghatti gum, mucilage of isapol husks, carboxymethylcellulose, methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl

methylcellulose, microcrystalline cellulose, cellulose acetate, poly(vinyl-pyrrolidone), magnesium aluminum silicate (Veegum), and larch arabogalactan), alginates, polyethylene oxide, polyethylene glycol, inorganic calcium salts, silicic acid, polymethacrylates, waxes, water, alcohol, and/or mixtures thereof.

[00370] Exemplary preservatives include antioxidants, chelating agents, antimicrobial preservatives, antifungal preservatives, alcohol preservatives, acidic preservatives, and other preservatives.

[00371] Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium

metabisulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabisulfite, and sodium sulfite.

[00372] Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g. , sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g. , citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal. [00373] Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid.

[00374] Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol. Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, beta-carotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid.

[00375] Other preservatives include tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT),

ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabisulfite, potassium sulfite, potassium metabisulfite, Glydant Plus, Phenonip, methylparaben, Germall 115, Germaben II, Neolone, Kathon, and Euxyl. In certain embodiments, the preservative is an anti-oxidant. In other embodiments, the preservative is a chelating agent.

[00376] Exemplary buffering agents include citrate buffer solutions, acetate buffer solutions, phosphate buffer solutions, ammonium chloride, calcium carbonate, calcium chloride, calcium citrate, calcium glubionate, calcium gluceptate, calcium gluconate, D- gluconic acid, calcium glycerophosphate, calcium lactate, propanoic acid, calcium levulinate, pentanoic acid, dibasic calcium phosphate, phosphoric acid, tribasic calcium phosphate, calcium hydroxide phosphate, potassium acetate, potassium chloride, potassium gluconate, potassium mixtures, dibasic potassium phosphate, monobasic potassium phosphate, potassium phosphate mixtures, sodium acetate, sodium bicarbonate, sodium chloride, sodium citrate, sodium lactate, dibasic sodium phosphate, monobasic sodium phosphate, sodium phosphate mixtures, tromethamine, magnesium hydroxide, aluminum hydroxide, alginic acid, pyrogen-free water, isotonic saline, Ringer's solution, ethyl alcohol, and mixtures thereof.

[00377] Exemplary lubricating agents include magnesium stearate, calcium stearate, stearic acid, silica, talc, malt, glyceryl behanate, hydrogenated vegetable oils, polyethylene glycol, sodium benzoate, sodium acetate, sodium chloride, leucine, magnesium lauryl sulfate, sodium lauryl sulfate, and mixtures thereof.

[00378] Exemplary natural oils include almond, apricot kernel, avocado, babassu, bergamot, black current seed, borage, cade, camomile, canola, caraway, carnauba, castor, cinnamon, cocoa butter, coconut, cod liver, coffee, corn, cotton seed, emu, eucalyptus, evening primrose, fish, flaxseed, geraniol, gourd, grape seed, hazel nut, hyssop, isopropyl myristate, jojoba, kukui nut, lavandin, lavender, lemon, litsea cubeba, macademia nut, mallow, mango seed, meadowfoam seed, mink, nutmeg, olive, orange, orange roughy, palm, palm kernel, peach kernel, peanut, poppy seed, pumpkin seed, rapeseed, rice bran, rosemary, safflower, sandalwood, sasquana, savoury, sea buckthorn, sesame, shea butter, silicone, soybean, sunflower, tea tree, thistle, tsubaki, vetiver, walnut, and wheat germ oils.

Exemplary synthetic oils include, but are not limited to, butyl stearate, caprylic triglyceride, capric triglyceride, cyclomethicone, diethyl sebacate, dimethicone 360, isopropyl myristate, mineral oil, octyldodecanol, oleyl alcohol, silicone oil, and mixtures thereof.

[00379] Liquid dosage forms for oral and parenteral administration include

pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active ingredients, the liquid dosage forms may comprise inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol, dimethylformamide, oils (e.g., cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents. In certain embodiments for parenteral administration, the compounds described herein are mixed with solubilizing agents such as Cremophor™, alcohols, oils, modified oils, glycols, polysorbates, cyclodextrins, polymers, and mixtures thereof.

[00380] Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions can be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1 ,3-butanediol. Among the acceptable vehicles and solvents that can be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectables.

[00381] The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use. [00382] In order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This can be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle.

[00383] Compositions for rectal or vaginal administration are typically suppositories which can be prepared by mixing the compounds described herein with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active ingredient.

[00384] Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active ingredient is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may comprise buffering agents.

[00385] Solid compositions of a similar type can be employed as fillers in soft and hard- filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. Solid compositions of a similar type can be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.

[00386] The active ingredient can be in micro-encapsulated form with one or more excipients as noted above. The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings, release controlling coatings and other coatings well known in the pharmaceutical formulating art. In such solid dosage forms the active ingredient can be admixed with at least one inert diluent such as sucrose, lactose, or starch. Such dosage forms may comprise, as is normal practice, additional substances other than inert diluents, e.g., tableting lubricants and other tableting aids such a magnesium stearate and micro crystalline cellulose. In the case of capsules, tablets, and pills, the dosage forms may comprise buffering agents. They may optionally comprise opacifying agents and can be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes.

[00387] Dosage forms for topical and/or transdermal administration of a provided compound may include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants and/or patches. Generally, the active ingredient is admixed under sterile conditions with a pharmaceutically acceptable carrier and/or any desired preservatives and/or buffers as can be required. Additionally, the present disclosure encompasses the use of transdermal patches, which often have the added advantage of providing controlled delivery of an active ingredient to the body. Such dosage forms can be prepared, for example, by dissolving and/or dispensing the active ingredient in the proper medium. Alternatively or additionally, the rate can be controlled by either providing a rate controlling membrane and/or by dispersing the active ingredient in a polymer matrix and/or gel.

[00388] Formulations suitable for topical administration include, but are not limited to, liquid and/or semi liquid preparations such as liniments, lotions, oil in water and/or water in oil emulsions such as creams, ointments and/or pastes, and/or solutions and/or suspensions. Topically-administrable formulations may, for example, comprise from about 1% to about 10% (w/w) active ingredient, although the concentration of the active ingredient can be as high as the solubility limit of the active ingredient in the solvent. Formulations for topical administration may further comprise one or more of the additional ingredients described herein. [00389] A provided pharmaceutical composition can be prepared, packaged, and/or sold in a formulation suitable for pulmonary administration via the buccal cavity. Such a

formulation may comprise dry particles which comprise the active ingredient and which have a diameter in the range from about 0.5 to about 7 nanometers or from about 1 to about 6 nanometers. Such compositions are conveniently in the form of dry powders for

administration using a device comprising a dry powder reservoir to which a stream of propellant can be directed to disperse the powder and/or using a self-propelling

solvent/powder dispensing container such as a device comprising the active ingredient dissolved and/or suspended in a low-boiling propellant in a sealed container. Such powders comprise particles wherein at least 98% of the particles by weight have a diameter greater than 0.5 nanometers and at least 95% of the particles by number have a diameter less than 7 nanometers. Alternatively, at least 95% of the particles by weight have a diameter greater than 1 nanometer and at least 90% of the particles by number have a diameter less than 6 nanometers. Dry powder compositions may include a solid fine powder diluent such as sugar and are conveniently provided in a unit dose form.

[00390] Low boiling propellants generally include liquid propellants having a boiling point of below 65 °F at atmospheric pressure. Generally the propellant may constitute 50 to 99.9% (w/w) of the composition, and the active ingredient may constitute 0.1 to 20%> (w/w) of the composition. The propellant may further comprise additional ingredients such as a liquid non-ionic and/or solid anionic surfactant and/or a solid diluent (which may have a particle size of the same order as particles comprising the active ingredient).

[00391] Pharmaceutical compositions formulated for pulmonary delivery may provide the active ingredient in the form of droplets of a solution and/or suspension. Such formulations can be prepared, packaged, and/or sold as aqueous and/or dilute alcoholic solutions and/or suspensions, optionally sterile, comprising the active ingredient, and may conveniently be administered using any nebulization and/or atomization device. Such formulations may further comprise one or more additional ingredients including, but not limited to, a flavoring agent such as saccharin sodium, a volatile oil, a buffering agent, a surface active agent, and/or a preservative such as methylhydroxybenzoate. The droplets provided by this route of administration may have an average diameter in the range from about 0.1 to about 200 nanometers.

[00392] Formulations described herein as being useful for pulmonary delivery are useful for intranasal delivery of a pharmaceutical composition. Another formulation suitable for intranasal administration is a coarse powder comprising the active ingredient and having an average particle from about 0.2 to 500 micrometers. Such a formulation is administered by rapid inhalation through the nasal passage from a container of the powder held close to the nares.

[00393] Formulations for nasal administration may, for example, comprise from about as little as 0.1% (w/w) and as much as 100% (w/w) of the active ingredient, and may comprise one or more of the additional ingredients described herein. A provided pharmaceutical composition can be prepared, packaged, and/or sold in a formulation for buccal

administration. Such formulations may, for example, be in the form of tablets and/or lozenges made using conventional methods, and may contain, for example, 0.1 to 20%> (w/w) active ingredient, the balance comprising an orally dissolvable and/or degradable

composition and, optionally, one or more of the additional ingredients described herein. Alternately, formulations for buccal administration may comprise a powder and/or an aerosolized and/or atomized solution and/or suspension comprising the active ingredient. Such powdered, aerosolized, and/or aerosolized formulations, when dispersed, may have an average particle and/or droplet size in the range from about 0.1 to about 200 nanometers, and may further comprise one or more of the additional ingredients described herein.

[00394] A provided pharmaceutical composition can be prepared, packaged, and/or sold in a formulation for ophthalmic administration. Such formulations may, for example, be in the form of eye drops including, for example, a 0.1/1.0%) (w/w) solution and/or suspension of the active ingredient in an aqueous or oily liquid carrier. Such drops may further comprise buffering agents, salts, and/or one or more other of the additional ingredients described herein. Other opthalmically-administrable formulations which are useful include those which comprise the active ingredient in microcrystalline form and/or in a liposomal preparation. Ear drops and/or eye drops are contemplated as being within the scope of this disclosure.

[00395] Although the descriptions of pharmaceutical compositions provided herein are principally directed to pharmaceutical compositions which are suitable for administration to humans, it will be understood by the skilled artisan that such compositions are generally suitable for administration to animals of all sorts. Modification of pharmaceutical compositions suitable for administration to humans in order to render the compositions suitable for administration to various animals is well understood, and the ordinarily skilled veterinary pharmacologist can design and/or perform such modification with ordinary experimentation. [00396] Compounds provided herein are typically formulated in dosage unit form for ease of administration and uniformity of dosage. It will be understood, however, that the total daily usage of provided compositions will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular subject or organism will depend upon a variety of factors including the disease, disorder, or condition being treated and the severity of the disorder; the activity of the specific active ingredient employed; the specific composition employed; the age, body weight, general health, sex and diet of the subject; the time of administration, route of administration, and rate of excretion of the specific active ingredient employed; the duration of the treatment; drugs used in combination or coincidental with the specific active ingredient employed; and like factors well known in the medical arts.

[00397] The compounds and compositions provided herein can be administered by any route, including enteral (e.g., oral), parenteral, intravenous, intramuscular, intra-arterial, intramedullary, intrathecal, subcutaneous, intraventricular, transdermal, interdermal, rectal, intravaginal, intraperitoneal, topical (as by powders, ointments, creams, and/or drops), mucosal, nasal, bucal, sublingual; by intratracheal instillation, bronchial instillation, and/or inhalation; and/or as an oral spray, nasal spray, and/or aerosol. Specifically contemplated routes are oral administration, intravenous administration (e.g., systemic intravenous injection), regional administration via blood and/or lymph supply, and/or direct

administration to an affected site. In general the most appropriate route of administration will depend upon a variety of factors including the nature of the agent (e.g., its stability in the environment of the gastrointestinal tract), and/or the condition of the subject (e.g., whether the subject is able to tolerate oral administration).

[00398] The exact amount of a compound required to achieve an effective amount will vary from subject to subject, depending, for example, on species, age, and general condition of a subject, severity of the side effects or disorder, identity of the particular compound(s), mode of administration, and the like. The desired dosage can be delivered three times a day, two times a day, once a day, every other day, every third day, every week, every two weeks, every three weeks, or every four weeks. In certain embodiments, the desired dosage can be delivered using multiple administrations (e.g., two, three, four, five, six, seven, eight, nine, ten, eleven, twelve, thirteen, fourteen, or more administrations).

[00399] In certain embodiments, an effective amount of a compound for administration one or more times a day to a 70 kg adult human may comprise about 0.0001 mg to about 3000 mg, about 0.0001 mg to about 2000 mg, about 0.0001 mg to about 1000 mg, about 0.001 mg to about 1000 mg, about 0.01 mg to about 1000 mg, about 0.1 mg to about 1000 mg, about 1 mg to about 1000 mg, about 1 mg to about 100 mg, about 10 mg to about 1000 mg, or about 100 mg to about 1000 mg, of a compound per unit dosage form.

[00400] In certain embodiments, a compound described herein may be administered at dosage levels sufficient to deliver from about 0.001 mg/kg to about 1000 mg/kg, from about 0.01 mg/kg to about mg/kg, from about 0.1 mg/kg to about 40 mg/kg, from about 0.5 mg/kg to about 30 mg/kg, from about 0.01 mg/kg to about 10 mg/kg, from about 0.1 mg/kg to about 10 mg/kg, or from about 1 mg/kg to about 25 mg/kg, of subject body weight per day, one or more times a day, to obtain the desired therapeutic effect.

[00401] In some embodiments, a compound described herein is administered one or more times per day, for multiple days. In some embodiments, the dosing regimen is continued for days, weeks, months, or years.

[00402] It will be appreciated that dose ranges as described herein provide guidance for the administration of provided pharmaceutical compositions to an adult. The amount to be administered to, for example, a child or an adolescent can be determined by a medical practitioner or person skilled in the art and can be lower or the same as that administered to an adult.

[00403] It will be also appreciated that a compound or composition, as described herein, can be administered in combination with one or more additional therapeutically active agents. In certain embodiments, a compound or composition provided herein is administered in combination with one or more additional therapeutically active agents that improve its bioavailability, reduce and/or modify its metabolism, inhibit its excretion, and/or modify its distribution within the body. It will also be appreciated that the therapy employed may achieve a desired effect for the same disorder, and/or it may achieve different effects.

[00404] The compound or composition can be administered concurrently with, prior to, or subsequent to, one or more additional therapeutically active agents. In certain embodiments, the additional therapeutically active agent is a compound of Formula (I). In certain embodiments, the additional therapeutically active agent is not a compound of Formula (I). In general, each agent will be administered at a dose and/or on a time schedule determined for that agent. In will further be appreciated that the additional therapeutically active agent utilized in this combination can be administered together in a single composition or administered separately in different compositions. The particular combination to employ in a regimen will take into account compatibility of a provided compound with the additional therapeutically active agent and/or the desired therapeutic effect to be achieved. In general, it is expected that additional therapeutically active agents utilized in combination be utilized at levels that do not exceed the levels at which they are utilized individually. In some embodiments, the levels utilized in combination will be lower than those utilized

individually.

[00405] Exemplary additional therapeutically active agents include, but are not limited to, small organic molecules such as drug compounds (e.g., compounds approved by the U.S. Food and Drug Administration as provided in the Code of Federal Regulations (CFR)), peptides, proteins, carbohydrates, monosaccharides, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic polypeptides or proteins, small molecules linked to proteins, glycoproteins, steroids, nucleic acids, DNAs, RNAs, nucleotides, nucleosides, oligonucleotides, antisense oligonucleotides, lipids, hormones, vitamins, and cells.

[00406] Also encompassed by the present discosure are kits (e.g., pharmaceutical packs). The kits provided may comprise a provided pharmaceutical composition or compound and a container (e.g., a vial, ampule, bottle, syringe, and/or dispenser package, or other suitable container). In some embodiments, provided kits may optionally further include a second container comprising a pharmaceutical excipient for dilution or suspension of a provided pharmaceutical composition or compound. In some embodiments, a provided pharmaceutical composition or compound provided in the container and the second container are combined to form one unit dosage form. In some embodiments, a provided kits further includes instructions for use.

[00407] Compounds and compositions described herein are generally useful for the inhibition of CARM1. In some embodiments, the CARMl is human CARMl . In some embodiments, methods of treating CARMl -mediated disorder in a subject are provided which comprise administering an effective amount of a compound described herein (e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof), to a subject in need of treatment. In certain embodiments, the effective amount is a therapeutically effective amount. In certain embodiments, the effective amount is a prophylactically effective amount. In certain embodiments, the subject is suffering from a CARMl -mediated disorder. In certain embodiments, the subject is susceptible to a CARMl -mediated disorder.

[00408] As used herein, the term "CARMl -mediated disorder" means any disease, disorder, or other pathological condition in which CARMl is known to play a role.

Accordingly, in some embodiments, the present disclosure relates to treating or lessening the severity of one or more diseases in which CARMl is known to play a role. [00409] In some embodiments, the present disclosure provides a method of inhibiting CARMl comprising contacting CARMl with an effective amount of a compound described herein, e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof. The CARMl may be purified or crude, and may be present in a cell, tissue, or subject. Thus, such methods encompass both inhibition of in vitro and in vivo CARMl activity. In certain embodiments, the method is an in vitro method, e.g., such as an assay method. It will be understood by one of ordinary skill in the art that inhibition of CARMl does not necessarily require that all of the CARMl be occupied by an inhibitor at once. Exemplary levels of inhibition of CARMl include at least 10% inhibition, about 10% to about 25% inhibition, about 25% to about 50% inhibition, about 50% to about 75% inhibition, at least 50% inhibition, at least 75% inhibition, about 80% inhibition, about 90% inhibition, and greater than 90% inhibition.

[00410] In some embodiments, provided is a method of inhibiting CARMl activity in a subject in need thereof comprising administering to the subject an effective amount of a compound described herein (e.g., a compound of Formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof.

[00411] In certain embodiments, provided is a method of modulating gene expression or activity in a cell which comprises contacting a cell with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof. In certain embodiments, the cell in culture in vitro. In certain embodiments, the cell is in an animal, e.g., a human. In certain embodiments, the cell is in a subject in need of treatment.

[00412] In certain embodiments, provided is a method of modulating transcription in a cell which comprises contacting a cell with an effective amount of a compound of Formula (I), or a pharmaceutically acceptable salt thereof. In certain embodiments, the cell in culture in vitro. In certain embodiments, the cell is in an animal, e.g., a human. In certain

embodiments, the cell is in a subject in need of treatment.

[00413] In certain embodiments, a method is provided of selecting a therapy for a subject having a disease associated with CARMl -mediated disorder or mutation comprising the steps of determining the presence of CARMl -mediated disorder or gene mutation in the CARMl gene or and selecting, based on the presence of CARMl -mediated disorder a gene mutation in the CARMl gene a therapy that includes the administration of a provided compound. In certain embodiments, the disease is cancer.

[00414] In certain embodiments, a method of treatment is provided for a subject in need thereof comprising the steps of determining the presence of CARMl -mediated disorder or a gene mutation in the CARMl gene and treating the subject in need thereof, based on the presence of a CARMl -mediated disorder or gene mutation in the CARMl gene with a therapy that includes the administration of a provided compound. In certain embodiments, the subject is a cancer patient.

[00415] In some embodiments, a compound provided herein is useful in treating a proliferative disorder, such as cancer. For example, while not being bound to any particular mechanism, protein arginine methylation by CARMl is a modification that has been implicated in signal transduction, gene transcription, DNA repair and mRNA splicing, among others; and overexpression of CARMl within these pathways is often associated with various cancers. Thus, compounds which inhibit the action of PRMTs, and specifically CARMl, as provided herein, are effective in the treatment of cancer.

[00416] In some embodiments, compounds provided herein are effective in treating cancer through the inhibition of CARMl . For example, CARMl levels have been shown to be elevated in castration-resistant prostate cancer (CRPC) (e.g., see Di Lorenzo et al., Drugs (2010) 70:983-1000), as well as in aggressive breast tumors (Hong et al, Cancer 2004 101, 83-89; El Messaoudi et al, Proc. Natl. Acad. Sci. U. S. A. 2006, 103, 13351-13356;

Majumder et al, Prostate 2006 66, 1292-1301). Thus, in some embodiments, inhibitors of CARMl, as described herein, are useful in treating cancers associated with aberrant CARMl activity, e.g., CARMl overexpression or aberrant protein methylation. For example, aberrant CARMl activity has been found in prostate cancer {e.g., see Hong et al, Cancer (2004), 101 :83-89); plays a coactivator role in the dysragulation of beta-catenin activity in colorectal cancer {e.g., see Ou et al, Mol. Cancer Res. (2011) 9:660); and has been linked to estrogen signaling and estrogen related cancers such as breast cancer (see, e.g., Teyssiewr et al, Trends in Endocrinology and Metabolism (2010) 21 : 181-189). CARMl has also been shown to affect estrogen receptor alpha (ER-alpha) dependent breast cancer cell differentiation and proliferation (Al-Dhaheri et al., Cancer Res. 2011 71, 2118-2128), thus in some aspects CARMl inhibitors, as described herein, are useful in treating ERa-dependent breast cancer by inhibiting cell differentiation and proliferation. In another example, CARMl has been shown to be recruited to the promoter of E2F1 (which encodes a cell cycle regulator) as a transcriptional co-activator (Frietze et al., Cancer Res. 2008 68, 301-306). Thus, CARMl - mediated upregulation of E2F1 expression may contribute to cancer progression and chemoresistance as increased abundance of E2F1 triggers invasion and metastasis by activating growth receptor signaling pathways, which in turn promote an antiapoptotic tumor environment (Engelmann and Piitzer, Cancer Res 2012 72; 571). Accordingly, in some embodiments, the inhibition of CARMl, e.g., by compounds provided herein, is useful in treating cancers associated with E2F1 upregulation, e.g., such as lung cancer (see, e.g., Eymin et al, Oncogene (2001) 20: 1678-1687), and breast cancer (see, e.g., Brietz et al, Cancer Res. (2008) 68:301-306). Thus, without being bound by any particular mechanism, the inhibition of CARMl, e.g., by compounds described herein, is beneficial in the treatment of cancer. CARMl overexpression has also been demonstrated to be elevated in 75% of colorectal cancers (Kim et al., BMC Cancer, 10, 197). It has been additionally been determined that depletion of CARMl in WNT/ -catenin dysregulated colorectal cancer suppressed anchorage independent growth (Ou et al, Mol. Cancer. Res., 2011 9, 660-670). This, in some embodiments, the inhibition of CARMl, e.g. by compounds provided herein, is useful in colorectal cancer associated with elevated CARMl expression or dysregulated WNT/ -catenin signaling.

[00417] In some embodiments, compounds described herein are useful for treating a cancer including, but not limited to, acoustic neuroma, adenocarcinoma, adrenal gland cancer, anal cancer, angiosarcoma {e.g., lymphangiosarcoma, lymphangioendotheliosarcoma,

hemangiosarcoma), appendix cancer, benign monoclonal gammopathy, biliary cancer {e.g., cholangiocarcinoma), bladder cancer, breast cancer {e.g., adenocarcinoma of the breast, papillary carcinoma of the breast, mammary cancer, medullary carcinoma of the breast), brain cancer {e.g., meningioma; glioma, e.g., astrocytoma, oligodendroglioma;

medulloblastoma), bronchus cancer, carcinoid tumor, cervical cancer {e.g., cervical adenocarcinoma), choriocarcinoma, chordoma, craniopharyngioma, colorectal cancer {e.g., colon cancer, rectal cancer, colorectal adenocarcinoma), epithelial carcinoma, ependymoma, endotheliosarcoma {e.g., Kaposi's sarcoma, multiple idiopathic hemorrhagic sarcoma), endometrial cancer {e.g., uterine cancer, uterine sarcoma), esophageal cancer {e.g., adenocarcinoma of the esophagus, Barrett's adenocarinoma), Ewing sarcoma, eye cancer {e.g., intraocular melanoma, retinoblastoma), familiar hypereosinophilia, gall bladder cancer, gastric cancer {e.g., stomach adenocarcinoma), gastrointestinal stromal tumor (GIST), head and neck cancer {e.g., head and neck squamous cell carcinoma, oral cancer {e.g., oral squamous cell carcinoma (OSCC), throat cancer {e.g., laryngeal cancer, pharyngeal cancer, nasopharyngeal cancer, oropharyngeal cancer)), hematopoietic cancers {e.g., leukemia such as acute lymphocytic leukemia (ALL) {e.g., B-cell ALL, T-cell ALL), acute myelocytic leukemia (AML) {e.g., B-cell AML, T-cell AML), chronic myelocytic leukemia (CML) {e.g., B-cell CML, T-cell CML), and chronic lymphocytic leukemia (CLL) (e.g., B-cell CLL, T- cell CLL); lymphoma such as Hodgkin lymphoma (HL) (e.g., B-cell HL, T-cell HL) and non-Hodgkin lymphoma (NHL) (e.g., B-cell NHL such as diffuse large cell lymphoma (DLCL) (e.g., diffuse large B-cell lymphoma (DLBCL)), follicular lymphoma, chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma (MCL), marginal zone B-cell lymphomas (e.g., mucosa-associated lymphoid tissue (MALT) lymphomas, nodal marginal zone B-cell lymphoma, splenic marginal zone B-cell

lymphoma), primary mediastinal B-cell lymphoma, Burkitt lymphoma, lymphoplasmacytic lymphoma (i.e., "Waldenstrom's macroglobulinemia"), hairy cell leukemia (HCL), immunoblastic large cell lymphoma, precursor B-lymphoblastic lymphoma and primary central nervous system (CNS) lymphoma; and T-cell NHL such as precursor T-lymphoblastic lymphoma/leukemia, peripheral T-cell lymphoma (PTCL) (e.g., cutaneous T-cell lymphoma (CTCL) (e.g., mycosis fungiodes, Sezary syndrome), angioimmunoblastic T-cell lymphoma, extranodal natural killer T-cell lymphoma, enteropathy type T-cell lymphoma, subcutaneous panniculitis-like T-cell lymphoma, anaplastic large cell lymphoma); a mixture of one or more leukemia/lymphoma as described above; and multiple myeloma (MM)), heavy chain disease (e.g., alpha chain disease, gamma chain disease, mu chain disease), hemangioblastoma, inflammatory myofibroblastic tumors, immunocytic amyloidosis, kidney cancer (e.g., nephroblastoma a.k.a. Wilms' tumor, renal cell carcinoma), liver cancer (e.g., hepatocellular cancer (HCC), malignant hepatoma), lung cancer (e.g., bronchogenic carcinoma, small cell lung cancer (SCLC), non-small cell lung cancer (NSCLC), adenocarcinoma of the lung), leiomyosarcoma (LMS), mastocytosis (e.g., systemic mastocytosis), myelodysplasia syndrome (MDS), mesothelioma, myeloproliferative disorder (MPD) (e.g., polycythemia Vera (PV), essential thrombocytosis (ET), agnogenic myeloid metaplasia (AMM) a.k.a. myelofibrosis (MF), chronic idiopathic myelofibrosis, chronic myelocytic leukemia (CML), chronic neutrophilic leukemia (CNL), hypereosinophilic syndrome (HES)), neuroblastoma, neurofibroma (e.g., neurofibromatosis (NF) type 1 or type 2, schwannomatosis),

neuroendocrine cancer (e.g., gastroenteropancreatic neuroendocrine tumor (GEP-NET), carcinoid tumor), osteosarcoma, ovarian cancer (e.g., cystadenocarcinoma, ovarian embryonal carcinoma, ovarian adenocarcinoma), papillary adenocarcinoma, pancreatic cancer (e.g., pancreatic andenocarcinoma, intraductal papillary mucinous neoplasm (IPMN), Islet cell tumors), penile cancer (e.g., Paget's disease of the penis and scrotum), pinealoma, primitive neuroectodermal tumor (PNT), prostate cancer (e.g., prostate adenocarcinoma), rectal cancer, rhabdomyosarcoma, salivary gland cancer, skin cancer (e.g., squamous cell carcinoma (SCC), kerato acanthoma (KA), melanoma, basal cell carcinoma (BCC)), small bowel cancer (e.g., appendix cancer), soft tissue sarcoma (e.g., malignant fibrous

histiocytoma (MFH), liposarcoma, malignant peripheral nerve sheath tumor (MPNST), chondrosarcoma, fibrosarcoma, myxosarcoma), sebaceous gland carcinoma, sweat gland carcinoma, synovioma, testicular cancer (e.g., seminoma, testicular embryonal carcinoma), thyroid cancer (e.g., papillary carcinoma of the thyroid, papillary thyroid carcinoma (PTC), medullary thyroid cancer), urethral cancer, vaginal cancer, and vulvar cancer (e.g., Paget 's disease of the vulva).

[00418] In certain embodiments, the cancer is a solid cancer. In certain embodiments, the cancer is a liquid cancer.

[00419] In certain embodiments, the cancer is breast cancer, prostate cancer, colorectal cancer, or a hematopoietic cancer (e.g., multiple myeloma).

[00420] CARMl is also the most abundant PRMT expressed in skeletal muscle cells, and has been found to selectively control the pathways modulating glycogen metabolism, and associated AMPK (AMP -activated protein kinase) and p38 MAPK (mitogen-activated protein kinase) expression. See, e.g., Wang et ah, Biochem (2012) 444:323-331. Thus, in some embodiments, inhibitors of CARMl, as described herein, are useful in treating metabolic disorders, e.g., for example skeletal muscle metabolic disorders, e.g., glycogen and glucose metabolic disorders. Exemplary skeletal muscle metabolic disorders include, but are not limited to, Acid Maltase Deficiency (Glycogenosis type 2; Pompe disease), Debrancher deficiency (Glycogenosis type 3), Phosphorylase deficiency (McArdle's; GSD 5), X-linked syndrome (GSD9D), Autosomal recessive syndrome (GSD9B), Tarui's disease (Glycogen storage disease VII; GSD 7), Phosphoglycerate Mutase deficiency (Glycogen storage disease X; GSDX; GSD 10), Lactate dehydrogenase A deficiency (GSD 11), Branching enzyme deficiency (GSD 4), Aldolase A (muscle) deficiency, β-Enolase deficiency, Triosephosphate isomerase (TIM) deficiency, Lafora's disease (Progressive myoclonic epilepsy 2), Glycogen storage disease (Muscle, Type 0, Phosphoglucomutase 1 Deficiency (GSD 14)), and

Glycogenin Deficiency (GSD 15).

EXAMPLES

[00421] In order that the invention described herein may be more fully understood, the following examples are set forth. It should be understood that these examples are for illustrative purposes only and are not to be construed as limiting this invention in any manner. Synthetic Methods

[00422] The synthesis of an exemplary set of compounds of Formula (I) is provided below. These compounds are also provided in Table 1.

Example 1. Preparation of methyl 2-(2-(5-((R)-2-hydroxy-3-

(methylamino)propoxy)phenyl)- 6-(l,4-dimethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-4- yl)-2,7-diazaspiro[3.5] nonane-7-carboxylate

[00423] Step 1: methyl 2-(2-(3-((R)-3-(tert-butoxycarbonyl(methyl)amino) -2-(tert- butyldimethylsilyloxy)propoxy)phenyl)-6-chloro-5-methylpyrimidin-4-yl)-2,7-diazaspiro

[3.5]nonane-7-carboxylate. To a solution of (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3- (3-(4,6-dichloro-5- methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (1 g, 1.8 mmol) in DMF (20 mL) was added methyl 2,7-diazaspiro[3.5]nonane-7-carboxylate TFA salt (2.15 g, 7.2 mmol) and triethylamine (909 mg, 9 mmol) at room temperature. The reaction mixture was heated at 110 °C for 16h, cooled down to room temperature, diluted with EtOAc (120 mL) and then washed with water (80 mL x 2) and brine (80 mL). The organic layer was dried over Na₂S0₄, filtered and concentrated in vacuo to render a residue which was purified by column chromatography over silicagel to give methyl 2-(2-(3-((R)-3-(tert- butoxycarbonyl(methyl)amino)-2-(tert-butyldimethylsilyloxy) propoxy)phenyl)-6-chloro-5- methylpyrimidin-4-yl)-2,7-diazaspiro [3.5] nonane-7-carboxylate as a yellow solid (1.2 g, 95% yield). ESI-LCMS (m/z): 704.3 found for [M+l]⁺.

[00424] Step 2: methyl 2-(2-(3-((R)-3-(tert-butoxycarbonyl(methyl)amino)-2 -(tert- butyldimethylsilyloxy)propoxy)phenyl)-6-(l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl pyrimidin-4-yl)-2,7-diazaspiro[3.5]nonane-7-carboxylate. To a solution of methyl 2-(2-(3- ((R)-3-(tert-butoxycarbonyl(methyl)amino)-2-(tert- butyldimethylsilyloxy)propoxy)phenyl)- 6-chloro-5-methylpyrimidin-4-yl)-2,7-diaza- spiro[3.5] nonane-7-carboxylate (160 mg, 0.23 mmol) in degassed dioxane:H₂0 (5: 1, 6 mL) was added l,4-dimethyl-5-(4,4,5,5-tetramethyl- l,3,2-dioxaborolan-2-yl)-lH-pyrazole (78 mg, 0.35 mmol), Pd(dppf)Cl₂ (16 mg, 0.02mmol) and Na₂C0₃ (73 mg, 0.69 mmol) at room temperature. The system was purged with N₂ and the mixture was stirred at 90°C for 3h. After being cooled down to room temperature the mixture was filtered through a pad of celite. The filtrate was concentrated and the resulting residue was purified by preparative-TLC on silicagel eluting with petroleum ether/EtOAc = 2/1 to give methyl 2-(2-(3- ((R)-3-(tert-butoxycarbonyl (methyl)amino)-2-(tert- butyldimethylsilyloxy)propoxy) phenyl)-6-(l,4-dimethyl-lH-pyrazol-5-yl)-5- methylpyrimidin-4-yl)-2,7-diazaspiro[3.5]nonane-7-carboxylate (130 mg, 74% yield) as a white solid. ESI-LCMS (m/z): 764.1 found for [M+l]⁺.

[00425] Step 3: methyl 2-(2-(5-((R)-2-hydroxy-3-(methylamino)propoxy) phenyl)-6- (l,4-dimethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-4-yl)-2,7-diazaspiro[3.5] nonane-7- carboxylate. A solution of methyl 2-(2-(3-((R)-3-(tert-butoxycarbonyl(methyl)amino)-2- (tert-butyldimethyl- silyloxy)propoxy)phenyl)-6-( 1 ,4-dimethyl- 1 H-pyrazol-5 -yl)-5 - methylpyrimidin-4-yl)-2,7-diazaspiro[3.5]nonane-7-carboxylate (130 mg, 0.17 mmol) in 90% TEA (2 mL), was stirred at room-temperature for lh. The solvent was then removed in vacuo and the resulting residue was dissolved in MeOH (5 mL). The solution was adjusted pH 7-8 with aqueous K₂C0₃ and concentrated. The residue was purified by preparative-HPLC to give methyl 2-(6-(l,4-dimethyl-lH-pyrazol-5-yl)-2-(3-((R)-2-hydroxy-3-(methylamino) propoxy) phenyl)-5-methylpyrimidin-4-yl)-2,7-diazaspiro[3.5]nonane-7-carboxylate as a white solid (20 mg, 21% ). ESI-LCMS (m/z): 550.2 found for [M+l]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 7.92-7.87 (m, 2H), 7.38 (s, 1H), 7.32 (t, J= 7.6 Hz, 1H), 7.03 (dd, J= 2.0 and 7.6 Hz, 1H), 4.23-4.12 (m, 4H), 4.10-4.07 (m, 1H), 4.03-3.98 (m, 2H), 3.73 (s, 3H), 3.67 (s, 3H), 3.55-3.45 (m, 4H), 2.86-2.70 (m, 2H), 2.44 (s, 3H), 2.08 (s, 3H), 1.98 (s, 3H) , 1.86- 1.78 (m, 4H).

Example 2. Preparation of (2R)-l-(4-chloro-3-(4-(4-chloro-l-methyl-lH-pyrazol-5-yl)-5- methyl-6-(5H-pyrrolo [3,4-b] pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl - amino)propan-2-ol.

[00426] Step 1: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-chloro- 6-(4-chloro-l-methyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)

phenoxy)propyl(methyl)carbamate . A solution of (R)-tert-butyl 2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 5 -methyl-6-(l -methyl- lH-pyrazol-5- yl)pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (120 mg, 0.19 mmol) and NCS (45 mg, 0.34 mmol) in DMF (2 ml) was stirred at room temperature for 2 h; the mixture was then diluted with water (10 mL) and extracted with EtOAc (10 mL x 3). The combined organic layers were washed with water (10 mL) and brine (10 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (2R)-2-(tert-butyl- dimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 6-(4-chloro- 1 -methyl- 1 H-pyrazol-5 -yl) -5 -methylpyrimidin-2-yl)phenoxy)propyl (methyl)carbamate (120 mg, 94% yield) as white solid. ESI-LCMS (m/z): 670 found for [M+l]⁺.

[00427] Step 2: tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-(4- chloro-l-methyl-lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl) pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 6-(4- chloro- 1 -methyl- 1 H-pyrazol-5 -yl)-5 -methylpyrimidin-2-yl)phenoxy)propyl

(methyl)carbamate (90 mg, 0.13 mmol); 6,7-dihydro -5H-pyrrolo[3,4-b]pyridine

dihydrochloride (or any other suitably substituted primary or secondary amine, 0.15 mmol), triethylamine (30 mg, 0.3 mmol) and n-BuOH (2 mL). The vessel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 110 °C. After being cooled down to room temperature, the mixture was diluted with water (20 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3- (4-(4-chloro-l -methyl- 1 H-pyrazol-5 -yl)-5-methyl-6- (5H-pyrrolo[3,4-b]pyridin-6(7H) -yl)pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate as a yellow solid (120 mg, crude), which was used directly into next step without further purification. ESI-LCMS (m/z): 754.0 found for [M+l]⁺.

[00428] Step 3: (2R)-l-(4-chloro-3-(4-(4-chloro-l-methyl-lH-pyrazol-5-yl)-5- methyl- 6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl- amino)propan-2-ol. A solution of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro- 3-(4-(4-chloro-l -methyl -lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)- yl)pyrimidin-2-yl) phenoxy) propyl(methyl)carbamate (120 mg, crude, from step 2) in a 4N HC1 solution in dioxane (6 mL), was stirred at room temperature for 1 h. The solvent was then removed in vacuo and the resulting residue was purified by preparative HPLC to give (2R)-l-(4-chloro-3-(4-(4-chloro-l- methyl-lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4- b]pyridin-6(7H)-yl)pyrimidin-2-yl) phenoxy)-3-(methylamino)propan-2-ol as a white solid (22 mg, 31% yield for 2 steps). ESI-LCMS (m/z): 540.2 found for [M+H]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 8.50 (d, J= 4.8 Hz, 1H), 7.88 (d, J= 7.6 Hz, 1H), 7.61 (s, 1H), 7.44- 7.38 (m, 2H), 7.35 (d, J= 3.2 Hz, 1H), 7.05(dd, J= 2.8 and 8.8 Hz, 1H), 5.44 (t, J= 14.8 Hz, 2H), 5.23 (t, J= 16.8 Hz, 2H), 4.15-4.08 (m, 1H), 4.06-3.98 (m, 2H), 3.89 (s, 3H), 2.86-2.73 (m, 2H), 2.51 (s, 3H), 2.47 (s, 3H). Example 3. Preparation of (2R)-l-(3-(4-(4-bromo-l-methyl-lH-pyrazol-5-yl)-5-methyl- 6-(5H -pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)-4-chlorophenoxy)-3-(methyl- amino)propan-2-ol

[00429] Step 1: (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4 -chloro- 5-methyl-6-(l-methyl-lH-pyrazol-5-yl)pyrimidin-2-yl)phenoxy)propyl

(methyl)carbamate. To a solution of (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3-(4- chloro-3-(4,6-di- chloro-5-methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (1.0 g, 1.7 mmol) in degassed dioxane and H₂0 (3/1, 24 niL) was added Na₂C0₃ (541 mg, 5.1 mmol), Pd(PPh₃)₄ (98 mg, 0.08 mmol) and l-methyl-5-(4,4,5,5-tetramethyl-l,3,2-dioxa- borolan-2-yl)-lH-pyrazole (707 mg, 3.4 mmol) at room temperature. The system was purged with N₂ and the mixture was stirred at 90 °C for 16h. After being cooled down to room temperature the solvent was removed in vacuo. The residue was diluted with water (30 mL) and extracted with EtOAc (100 mL x 2). The combined organic layers were washed with water (50 mL) and brine (50 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel (petroleum ether/EtOAc = 4/1) to give (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3- (4-chloro-3-(4-chloro-5-methyl-6-(l -methyl- lH-pyrazol-5-yl)pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (420 mg, 39 % yield). ESI-LCMS (m/z): 658.2 found for [M+23]⁺.

[00430] Step 2: tert-butyl (2R)-3-(3-(4-(4-bromo-l-methyl-lH-pyrazol-5-yl)- 6-chloro- 5-methylpyrimidin-2-yl)-4-chlorophenoxy)-2-(tert-butyldimethyl- silyloxy)propyl(methyl)carbamate. A solution of (R)-tert-butyl 2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 5 -methyl-6-(l -methyl- lH-pyrazol-5- yl)pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (120 mg, 0.19 mmol) and NBS (50 mg, 0.28 mmol) in DMF (3 ml) was stirred at room temperature for 2 h. After the reaction was complete the mixture was diluted with water (10 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (2R)-3-(3-(4-(4- bromo-1 -methyl- 1H- pyrazol-5-yl)-6-chloro-5-methylpyrimidin-2-yl)-4-chloro- phenoxy)-2-(tert-butyl

dimethylsilyloxy)propyl(methyl)carbamate (120 mg, 88% yield). ESI-LCMS (m/z): 736.1 found for [M+23]⁺.

[00431] Step 3: tert-butyl (2R)-3-(3-(4-(4-bromo-l-methyl-lH-pyrazol-5-yl)- 5- methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)-4-chlorophenoxy)-2-(tert- butyldimethylsilyloxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (2R)-3-(3-(4-(4-bromo-l-methyl-lH-pyrazol-5-yl)-6- chloro-5- methylpyrimidin-2-yl)-4-chlorophenoxy)-2-(tert-butyl dimethylsilyloxy)- propyl(methyl)carbamate (120 mg, 0.17 mmol); 6,7-dihydro -5H-pyrrolo[3,4-b]pyridine dihydrochloride (or any other suitably substituted primary or secondary amine, 0.34 mmol), triethylamine (0.5 mL, 3.5 mmol) and DMSO (3 mL). The vessel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 110 °C. After being cooled down to room temperature, the mixture was diluted with water (15 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel to give tert-butyl (2R)-3 -(3 -(4-(4-bromo-l -methyl- 1H- pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo [3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)-4- chlorophenoxy)-2-(tert-butyldimethyl- silyloxy)propyl(methyl)carbamate (120 mg, 89% yield) as white solid. ESI-LCMS (m/z): 798.2found for [M+H]⁺.

[00432] Step 4: (2R)-l-(3-(4-(4-bromo-l-methyl-lH-pyrazol-5-yl)-5-methyl- 6-(5H- pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)-4-chlorophenoxy)-3- (methylamino)propan-2-ol. A solution of tert-butyl (2R)-3-(3-(4-(4-bromo-l-methyl-lH- pyrazol-5-yl)-5-methyl-6-(5H- pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)-4- chlorophenoxy)-2-(tert-butyldi- methylsilyl oxy)propyl(methyl)carbamate (120 mg, 0.15 mmol) in 90% TFA (2 mL) was stirred at room temperature for 16h; concentrated in vacuo and the residue was dissolved in MeOH (2 ml); the resulting solution was adjusted to pH 7-8 with aqueous K₂CO₃ solution, filtered and the filtrate was concentrated again. The residue was purified by preparative-HPLC to give (2R)-1 -(3 -(4-(4-bromo-l -methyl- lH-pyrazol-5- yl)-5-methyl-6-(5H-pyrrolo[3,4-b] pyridin-6(7H)-yl)pyrimidin -2-yl)-4-chlorophenoxy)-3- (methylamino)propan-2-ol (26 mg, 30% yield ) as white solid. ESI-LCMS (m/z): 584.1 found for [M+H]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: : 8.50 (d, J= 5.2 Hz, 1H), 7.89 (d, J = 7.6 Hz, 1H), 7.64 (s, 1H), 7.45-7.39 (m, 2H) , 7.35 (d, J= 3.2 Hz, 1H), 7.07 (dd, J= 2.8 and 8.8 Hz, 1H), 5.43 (t, J= 14.8 Hz, 2H), 5.24 (t, J= 16.8 Hz, 2H), 4.14-4.10 (m, 1H), 4.05- 4.01 (m, 2H), 3.89 (s, 3H), 2.90-2.75 (m, 2H), 2.50 (s, 3H), 2.48 (s, 3H).

Example 4. Preparation of (2R)-l-(4-chloro-3-(4-(4-iodo-l-methyl-lH-pyrazol-5-yl)-5- methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl- amino)propan-2-ol

[00433] Step 1: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-chloro- 6-(4-iodo-l-methyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2- yl)phenoxy)propyl(methyl)carbamate. To a solution of (R)-tert-butyl 2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro -5 -methyl-6-(l -methyl- lH-pyrazol-5- yl)pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (220 mg, 0.35 mmol) in HO Ac (3 ml) was added NIS (117 mg, 0.52 mmol). The mixture was stirred at room temperature for 16h., diluted with water (10 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (2R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 6-(4-iodo- 1 -methyl- 1 H-pyrazol-5-yl)-5-methylpyrimidin-2- yl)phenoxy)propyl(methyl)carbamate (160 mg, 60% yield). ESI-LCMS (m/z): 784.0 found for [M+Na]⁺.

[00434] Step 2: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-(4- iodo-l-methyl-lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)- yl)pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4- chloro-6-(4-iodo-l -methyl- lH-pyrazol-5 -yl)-5-methylp yrimidin-2- yl)phenoxy)propyl(methyl)carbamate (160 mg, 0.2 mmol); 6,7-dihydro -5H-pyrrolo[3,4- b]pyridine dihydrochloride (or any other suitably substituted primary or secondary amine, 0.4 mmol), triethylamine (0.5 mL, 3.5 mmol) and DMSO (3 mL). The vessel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 110 °C. After being cooled down to room temperature, the mixture was diluted with water (15 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel to give tert-butyl (2R)-2-(tert-butyldimethyl-silyloxy)-3-(4-chloro-3-(4-(4-iodo-l -methyl- 1H- pyrazol-5-yl)-5-methyl-6-(5H- pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2- yl)phenoxy)propyl(methyl)carbamate (160 mg, 95% yield ). ESI-LCMS (m/z): 846 found for [M+H]⁺.

[00435] Step 3: (2R)-l-(4-chloro-3-(4-(4-iodo-l-methyl-lH-pyrazol-5-yl)-5- methyl-6- (5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl-amino)propan- 2-ol . A solution of tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-(4-iodo-l- methyl -lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl) phenoxy)propyl(methyl)carbamate (160 mg, 0.19 mmol) was treated with 90% TFA (2.2 mL), and the mixture was stirred at room temperature for 16h. The mixture was concentrated in vacuo and the residue was dissolved in MeOH (2 ml). The solution was adjusted to pH 7-8 with aqueous K₂C0₃ solution, then the mixture was filtered. The filtrate was concentrated, and the residue was purified by preparative HPLC to give (2R)-l-(4-chloro-3-(4-(4-iodo-l- methyl-lH -pyrazol-5-yl)-5-methyl-6-(5H- pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2- yl)phenoxy)-3-(methylamino) propan-2-ol (91 mg, 76% yield ). ESI-LCMS (m/z): 632.1 found for [M+H]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 8.50 (d, J= 4.8 Hz, 1H), 7.89 (d, J = 7.2 Hz, 1H), 7.65 (s, 1H), 7.43-7.38 (m, 2H) , 7.37 (d, J= 3.2 Hz, 1H), 7.05 (dd, J= 3.2 and 8.8 Hz, 1H), 5.45-5.37 (m, 2H), 5.30-5.21 (m, 2H), 4.12-4.09 (m, 1H), 4.06-3.98 (m, 2H), 3.90 (s, 3H), 2.84-2.71 (m, 2H), 2.47 (s, 3H), 2.46 (s, 3H).

Example 5. Preparation of (2R)-l-(4-chloro-3-(4-(4-chloro-l-ethyl-lH-pyrazol-5-yl)-5- methyl-6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl- amino)propan-2-ol

[00436] Step 1: tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(3-(4-chloro- 6-(l-ethyl- lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)phenoxy)propyl(methyl) carbamate. To a solution of (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3-(3-(4,6-dichloro-5- methyl- pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (1.0 g, 1.7 mmol) in degassed dioxane / H₂0 (5/1, 30 mL) was added l-ethyl-5-(4,4,5,5-tetra- methyl- l,2-oxaborolan-2-yl)-lH- pyrazole (420 mg, 1.9 mmol); Pd(PPh₃)₄ (104 mg, 0.09 mmol) and Na₂C0₃ (572 mg, 5.4 mmol) at room temperature. The system was purged with N₂ and the mixture was stirred at 90 °C for 16h. After being cooled down to room temperature, the solvent was removed in vacuo. The residue was diluted with water (30 mL) and extracted with EtOAc (100 mL x 2). The combined organic layers were washed with water (30 mL) and brine (30 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel (petroleum ether/EtOAc = 2/1) to give tert-butyl (R)-2-(tert-butyldimethyl- silyloxy)-3-(3-(4-chloro-6-(l-ethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)

phenoxy)propyl(methyl)carbamate (540 mg, white solid, 49% yield). ESI-LCMS (m/z): 638.4 found for [M+23]⁺.

[00437] Step 2: tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-chloro- 6-(4-chloro-l-ethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)phenoxy) propyl(methyl) carbamate. To a solution of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(3-(4-chloro-6- ( 1 - ethyl- 1 H-pyrazol-5 -yl)-5 -methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (400 mg, 0.65 mmol) in 30 mL of DMF was added NCS (389 mg, 2.92 mmol) and the mixture was heated at 70°C for 40 min; cooled down to room temperature, diluted with EtOAc (30 mL) and washed with water (30 mL x 2) and brine (30 mL). The organic layer was dried over Na₂S0₄, filtered and concentrated and the resulting residue was purified by preparative TLC (petroleum ether/EtOAc = 3/1) to give tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4- chloro-3-(4-chloro-6- (4-chloro-l -ethyl- 1 H-pyrazol-5 -yl)-5-methylpyrimidin-2- yl)phenoxy)propyl(methyl) carbamate (390 mg, white solid, 88% yield). ESI-LCMS (m/z): 630.4 found for [M-56]⁺.

[00438] Step 3: tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-(4- chloro-l-ethyl-lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridin-6 (7H)- yl)pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4- chloro-6-(4-chloro- 1 -ethyl- 1 H-pyrazol-5 -yl)-5 -methylpyrimidin-2-yl)phenoxy)propyl (methyl) carbamate (100 mg, 0.15 mmol); 6,7-dihydro -5H-pyrrolo[3,4-b]pyridine dihydrochloride (or any other suitably substituted primary or secondary amine, 0.35 mmol), KI (30 mg, 0.18 mmol), triethylamine (2 mL) and n-BuOH (4 mL). The vessel was capped, placed in a microwave reactor and irradiated for 2h. at external temperature of 140 °C. After being cooled down to room temperature, 30 mL of water was added and the mixture was extracted with EtOAc (40 mL x 3). The combined organic layers were washed with water (30 mL) and brine (30 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (R)-2- (tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-(4-chloro-l- ethyl- 1 H-pyrazol-5 -yl)-5-methyl- 6-(5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2- yl)phenoxy)propyl (methyl) carbamate (128 mg, crude), which was used for next step directly without further purification. ESI- LCMS (m/z): 768.4 found for [M+l]⁺.

[00439] Step 4: (2R)-l-(4-chloro-3-(4-(4-chloro-l-ethyl-lH-pyrazol-5-yl)-5- methyl-6- (5H-pyrrolo[3,4-b]pyridin-6(7H)-yl)pyrimidin-2-yl)phenoxy)-3-(methyl-amino)propan- 2-ol . A solution of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-(4-chloro - 1 -ethyl- 1 H-pyrazol-5 -yl)-5 -methyl-6-(5H-pyrrolo [3 ,4-b]pyridin-6(7H)-yl)pyrimidin-2- yl)phenoxy) propyl(methyl)carbamate (128 mg, crude from step 3) in MeOH (2 mL) was treated with 2 mL of 4N HC1 solution in dioxane and the mixture was stirred at room temperature for 2 h. The solvent was then removed in vacuo, the resulting residue was dissolved in MeOH (5 mL) and treated with ammonia till pH 8-9. The mixture was concentrated under vacuum and the residue was purified by preparative HPLC to give (2R)- l-(4-chloro-3-(4-(4-chloro-l -ethyl- lH-pyrazol-5-yl)-5-methyl-6-(5H-pyrrolo[3,4-b]pyridine- 6(7H)-yl) pyrimidin-2-yl)phenoxy)-3-(methylamino)propan-2-ol as white solid (60 mg, 72 % yield for two steps). ESI-LCMS: 554.0 found for [M+l]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 8.49 (d, J= 4.8 Hz, 1H), 7.87 (d, J= 7.2 Hz, 1H), 7.62 (s, 1H), 7.44-7.37 (m, 2H), 7.34 (d, J= 3.2 Hz, 1H), 7.05 (dd, J= 2.8 and 8.8 Hz, 1H), 5.43 (t, J= 15.2 Hz, 2H), 5.20 (t, J = 17.6 Hz, 2H), 4.35-4.26 (m, 1H), 4.21-4.10 (m, 2H), 4.06-3.98 (m, 2H), 2.88-2.74 (m, 2H), 2.49 (s, 3H), 2.48 (s, 3H), 1.36 (t, J= 7.2 Hz, 3H).

Example 6. Preparation of (2R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6- (6- (2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)phenoxy)-3- (methylamino)propan-2-ol

80 °C, o/n Step 2

Step 1

[00440] Step 1: tert-butyl 6-(2,2,2-trifluoroethyl)-2,6-diazaspiro [3.3] heptane -2- carboxylate. To a solution of tert-butyl 2,6-diazaspiro[3.3]heptane-2-carboxylate (200 mg, 1.01 mmol) in MeCN (6 mL) was added 3,3,3-trifluoropropyl trifluoromethanesulfonate (403 mg, 1.65 mmol) and CS₂CO₃ (804 mg, 2.47 mmol) at room temperature. The reaction mixture was stirred at 80 °C overnight, cooled down to room temperature, diluted with water (80 mL) and extracted with EtOAc (60 mL x 3). The combined organic layers were washed with water (30 mL) and brine (30 mL), dried over Na₂S0₄, filtered and concentrated in vacuo to give tert-butyl 6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptane-2-carboxylate as white solid (250 mg, crude), which was used into next step directly without further purification. ESI- LCMS (m/z): 281.1 found for [M+l]⁺.

[00441] Step 2: 2-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptane TFA salt. A solution of tert-butyl 6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptane-2-carboxylate (250 mg, crude from step 1) in TFA (5 mL) and water (0.5 ml) was stirred at room temperature for 2 h. The solvent was then removed in vacuo to give 2-(2,2,2-trifluoroethyl)-2,6-diaza- spiro[3.3]heptane 2,2,2-trifluoroacetate trifluoroacetate salt as brown solid (920 mg, crude), which was used into next step directly without further purification. ¹HNMR (400 MHz, CDCI₃) δ ppm: 4.36 (s, 4H), 4.29(s, 4H), 3.97-3.90 (m, 2H).

[00442] Step 3: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-(3,5- dimethylisoxazol-4-yl)-5-methyl-6-(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro [3.3]heptan-2- yl) pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4- chloro -6-(3,5-dimethylisoxazol-4-yl)-5-methyl pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (100 mg, 0.15 mmol); 2-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptane TFA salt (or any other suitably substituted primary or secondary amine, 1.54 mmol), triethylamine (186 mg, 1.85 mmol) and n-BuOH (1 mL). The vessel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 110 °C; cooled down to room temperature, diluted with water (70 mL) and extracted with EtOAc (60 mL x 3). The organic layers were combined, concentrated in vacuo and the residue was purified by preparative TLC developed with petroleum ether/EtOAc = 2/1 to give tert-butyl (2R)-2-(tert- butyldimethyl- silyloxy)-3-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6-(6-(2,2,2- trifluoro-ethyl)-2,6-diazaspiro[3 ]heptan-2-yl)pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate as light yellow solid (110 mg, 89.7 % ). ESI-LCMS (m/z): 795.3 found for

[M+l]⁺.

[00443] Step 4: (2R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6 -(6- (2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)phenoxy)-3- (methylamino)propan-2-ol. A solution of tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3- (4-chloro-3-(4-(3, 5 -dimethyl- isoxazol-4-yl)-5-methyl-6-(6-(2,2,2-trifluoroethyl)-2,6- diazaspiro[3.3]heptan-2-yl) pyrimidin-2-yl) phenoxy)propyl(methyl)carbamate (110 mg, 0.14 mmol) in TFA (5 mL) and water (0.5 ml), was stirred at 40 °C for 8 h. The solvent was then removed in vacuo, the residue was dissolved in MeOH (3 ml) and the solution was adjusted to pH 9 with ammonia. The solvent was removed in rotary evaporator and the residue was purified by preparative HPLC to give (2R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5- methyl-6-(6-(2,2,2-trifluoroethyl) -2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)phenoxy)- 3-(methylamino) propan-2-ol as a white solid (26 mg, 32%). ESI-LCMS (m/z): 581.1 found for [M+H]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 7.39 (d, J= 8.8 Hz, 1H), 7.19 (d, J= 2.8 Hz, 1H), 7.05 (dd, J = 3.2 and 8.8 Hz, 1H), 4.50 (s, 4H), 4.14-4.07 (m, 1H), 4.05-3.96 (m, 2H), 3.65 (s, 4H), 3.20-3.12 (m, 2H), 2.85-2.70 (m, 2H), 2.46 (s, 3H), 2.37 (s, 3H), 2.24 (s, 3H), 2.14 (s, 3H).

Example 7. Preparation of (2R)-l-(4-chloro-3-(4-(3-chloro-l,4-dimethyl-lH-pyrazol-5- yl)-5-methyl-6-(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2- yl)phenoxy)-3-(methylamino)propan-2-ol

[00444] Step 1: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro- 6-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)

phenoxy)propyl(methyl)carbamate. A solution of tert-butyl (2R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro-6-(l,4-dimethyl-lH-pyrazol-5-yl)-5- methylpyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (1.66 g, 2.55 mmol) and N- chlorosuccinimide (509 mg, 3.83 mmol) in DMF (20 mL) was stirred at room temperature for 12 h. After the reaction was complete, water (50 mL) and ethyl acetate (50 mL) were added. The organic layer was separated and washed with water (50 mL x 4) and brine (50 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel eluted with petroleum ether/ethyl acetate = 5: 1 to give tert-butyl (2R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro -6-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)- 5-methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (420 mg, 24% yield) as a colorless oil. ESI-LCMS (m/z): 706.2 found for [M+Na]⁺.

[00445] Step 2: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4-(3- chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl-6-(6-(2,2,2-trifluoroethyl)-2,6- diazaspiro [3.3] heptan-2-yl)pyrimidin-2-yl)phenoxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (2R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-chloro-6-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)- 5-methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (200 mg, 0.29 mmol); 2-(2,2,2- trifluoroethyl)-2,6-diazaspiro[3.3]heptane TFA salt (or any other suitably substituted primary or secondary amine, 0.35 mmol), DIPEA (151 mg, 1.17 mmol) and DMSO (4 mL). The veseel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 140 °C. After being cooled down to room temperature, the mixture was diluted with water (50 mL) and extracted with EtOAc (50 mL x 2). The combined organic layers were washed with water (50 mL x 4) and brine (40 mL), dried over Na₂S0₄, filtered and concentrated to give tert-butyl (2R)-2-(tert-butyl dimethyl silyloxy)-3-(4- chloro-3-(4-(3- chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl-6-(6-(2,2,2-trifluoro- ethyl)-2,6- diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)phenoxy)propyl (methyl) carbamate (270 mg, crude ) as a brown oil, which was used directly in the next step without further purification. ESI- LCMS (m/z): 828.0 found for [M+H]⁺.

[00446] Step 3: (2R)-l-(4-chloro-3-(4-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5- methyl-6-(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro 3.3]heptan-2-yl)pyrimidin-2- yl)phenoxy)-3-(methylamino)propan-2-ol. A solution of tert-butyl(2R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl- 6-(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2- yl)phenoxy)propyl(methyl)carbamate (270 mg, crude, from step 2) in 90% TEA (5 mL) was stirred at 30 °C for 3h. The solvent was then removed in vacuo, the residue was dissolved in MeOH (5 ml) and the solution was adjusted to pH 7-8 with ammonia. The solvent was removed in rotary evaporator and the residue was purified by preparative HPLC to give (2R)- l-(4-chloro-3-(4-(3-chloro-l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl-6- (6-(2,2,2- trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)phenoxy)-3- (methylamino)propan-2-ol as a white solid (27 mg, 15% yield for 2 steps ). ESI-LCMS (m/z): 614.0 found for [M+H]⁺; ¹HNMR (400 MHz, MeOD) δ ppm: 7.39 (d, J= 8.8 Hz, 1H), 7.21 (d, J = 3.2 Hz, 1H), 7.04 (dd, J= 3.2 and 8.8 Hz, 1H), 4.58-4.49 (m, 4H), 4.13-4.07 (m, 1H), 4.05-3.96 (m, 2H), 3.72 (s, 3H), 3.65 (s, 4H), 3.16 (q, J= 9.6 Hz, 2H), 2.86-2.72 (m, 2H), 2.48 (s, 3H), 2.13 (s, 3H), 1.95 (s, 3H).

Example 8. Preparation of (2R)-l-(3-(4-(3-bromo-l,4-dimethyl-lH-pyrazol-5-yl)- 5- methyl-6 -(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro [3.3] heptan-2-yl)pyrimidin-2-yl)-4- chlor -phenoxy)-3-(methylamino)propan-2-ol

[00447] Step 1: tert-butyl (2R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3- (4-chloro- 6-(l,4-dimethyl-lH-pyrazol-5-yl)-5-methylpyrimidin-2-yl)phenoxy)

propyl(methyl)carbamate. To a solution of (R)-tert-butyl 2-(tert-butyldimethylsilyloxy)-3- (4-chloro-3-(4,6- dichloro-5-methylpyrimidin-2-yl)phenoxy)propyl(methyl)carbamate (1.0 g, 1.7 mmol) in degassed dioxane and H₂0 (3/1, 20 niL) was added Na₂C0₃ (541 mg, 5.1 mmol), Pd(PPh₃)₄ (98 mg, 0.08 mmol) and l,4-dimethyl-5-(4,4,5,5-tetramethyl-l,3,2-dioxa- borolan-2-yl)-lH-pyrazole (755 mg, 3.4 mmol). The system was purged with N₂ and the mixture was stirred at 90 °C for 16h. After being cooled down to room temperature, the solvent was removed in vacuo. The residue was diluted with water (30 mL) and extracted with EtOAc (100 mL x 2). The combined organic layers were washed with water (30 mL) and brine (30 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by column chromatography over silicagel (petroleum ether/EtOAc = 4/1) to give tert-butyl (2R)- 2-(tert-butyldimethylsilyloxy)- 3-(4-chloro- 3-(4-chloro-6-(l,4-dimethyl-lH-pyrazol-5-yl)-5- methylpyrimidin-2-yl)phenoxy)propyl(methyl) carbamate (520 mg, 47 % yield) as white solid. ESI-LCMS (m/z): 650 found for [M+l]⁺.

[00448] Step 2: tert-butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethy l-lH-pyrazol-5 -yl)-6- chloro-5-methylpyrimidin-2-yl)-4-chlorophenoxy)-2-(tert-butyldimethyl- silyloxy)propyl(methyl)carbamate. A solution of (2R)-2-(tert-butyldimethylsilyloxy)-3-(4- chloro-3-(4-chloro-6-(l,4- dimethyl- lH-pyrazol-5 -yl)-5-methylpyrimidin-2- yl)phenoxy)propyl(methyl) carbamate (520 mg, 0.8 mmol) and NBS (470 mg, 2.64 mmol) in DMF (5 ml) was stirred at room temperature for 2h. The mixture was diluted with water (50 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated to give tert- butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethyl-lH-pyrazol-5-yl)-6-chloro-5-methylpyrimidin-2- yl)-4-chlorophenoxy)-2-(tert-butyldimethylsilyloxy)propyl(methyl)carbamate (600 mg, 103% yield). ESI-LCMS (m/z): 750 found for [M+23]⁺.

[00449] Step 3: tert-butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethyl-lH-pyrazol-5- yl)-5- methyl-6-(6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)-4- chlorophenoxy)-2-(tert-butyldimethylsilyloxy)propyl(methyl)carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethyl- lH-pyrazol-5-yl)-6- chloro-5-methylpyrimidin-2-yl)-4-chlorophenoxy)-2-(tert- butyldimethylsilyloxy) propyl(methyl)carbamate (130 mg, 0.18 mmol); 2-(2,2,2- trifluoroethyl)-2,6-diazaspiro[3.3]heptane TFA salt (or any other suitably substituted primary or secondary amine, 0.36 mmol), triethylamine (0.5 mL, 3.5 mmol). and DMSO (3 mL). The vessel was capped, placed in a microwave reactor and irradiated for 30 min. at external temperature of 110 °C. After being cooled down to room temperature, the mixture was diluted with water (15 mL) and extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (20 mL) and brine (20 mL), dried over Na₂S0₄, filtered and concentrated and the residue was submitted to purification by column chromatography over silicagel to give tert-butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethyl-lH-pyrazol-5-yl)-5-methyl-6- (6-(2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3] heptan-2-yl)pyrimidin-2-yl)-4-chlorophenoxy)-2- (tert-butyldimethylsilyloxy) propyl (methyl) carbamate (120 mg, 76% yield) as white solid. ESI-LCMS (m/z): 872.2found for [M+H]⁺.

[00450] Step 4: (2R)-l-(3-(4-(3-bromo-l,4-dimethyl-lH-pyrazol-5-yl)-5- methyl-6-(6- (2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)-4-chlorophenoxy)- 3-(methylamino)propan-2-ol. A solution of tert-butyl (2R)-3-(3-(4-(3-bromo-l,4-dimethyl- lH-pyrazol-5-yl)-5-methyl-6-(6- (2,2,2-trifluoroethyl)-2,6-diazaspiro[3.3]heptan-2- yl)pyrimidin-2-yl)-4-chlorophenoxy)-2-(tert-butyldimethylsilyloxy)propyl(methyl)carbamate (100 mg, 0.11 mmol) in 90% TFA (2 mL) was stirred at room temperature for 16h. After removal of voltiles in vacuo, the residue was dissolved in MeOH (2 ml), the solution pH was adjusted to 7-8 with aqueous K₂C0₃ solution; the mixture was filtered and the filtrate was concentrated. The resulting residue was purified by preparative HPLC to give (2R)-l-(3-(4- (3-bromo-l,4-dimethyl-lH-pyrazol-5-yl) -5-methyl-6-(6-(2,2,2-trif uoroethyl)-2,6- diazaspiro[3.3]heptan-2-yl)pyrimidin-2-yl)-4-chlorophenoxy)-3-(methyl amino)propan-2-ol (23 mg, 32% yield ) as white solid. ESI-LCMS (m/z): 658.1 found for [M+H]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: : 7.39 (d, J = 8.8 Hz, 1H), 7.21 (d, J = 2.8 Hz, 1H), 7.04 (dd, J =2.8 and 8.8 Hz, 1H) , 4.60-4.50 (m, 4H), 4.13-4.08 (m, 1H), 4.04-3.98 (m, 2H), 3.75 (s, 3H), 3.65 (s, 4H), 3.21-3.12 (m, 2H), 2.88-2.75 (m, 2H), 2.49 (s, 3H), 2.12 (s, 3H), 1.94 (s, 3H).

Example 9. Preparation of methyl (R)-5-(2-(2-chloro-5-(2-hydroxy-3- (methylamino)propoxy)phenyl)-6-(3,5-dimethylisoxazol-4-yl)-5-methylpyrimidin-4-yl)- 3,4,5,6-tetrahydropyrrolo [3,4-c] pyrrole-2(lH)-carboxylate

TFA

[00451] Step 1: 2-(tert-butyl) 5-methyl 4,6-dihydropyrrolo[3,4-c]pyrrole-2,5(lH,3H)- dicarboxylate. To a solution tert-butyl 3,4,5, 6-tetrahydropyrrolo[3,4-c]pyrrole-2(lH)- carboxylate (100 mg, 0.404 mmol) and triethylamine (82 mg, 0.80 mmol) in 5 niL of DCM was added and ClC0₂Me (58 mg, 0.61 mmol) and the mixture was stirred at room

temperature for lh. After the reaction was complete, water (10 mL) was added and the mixture was extracted with DCM (15 mL x 3). The combined organic layers were washed with brine (20 mL), dried over Na₂S0₄, filtered and concentrated to give 2-(tert-butyl) 5- methyl 4,6-dihydropyrrolo[3,4-c]pyrrole-2,5(lH,3H)-dicarboxylate (98 mg, 90% yield). ESI- LCMS (m/z): 269.7 found for [M+l]⁺.

[00452] Step 2: methyl 3,4,5,6-tetrahydropyrrolo [3,4-c] pyrrole-2(lH)-carboxylate TFA salt. A solution of 2-(tert-butyl) 5-methyl 4,6-dihydropyrrolo[3,4-c]pyrrole-2,5(lH,3H)- dicarboxylate (98 mg, 0.37 mmol) in DCM (3 mL) was treated with TFA (2 mL) and the mixture was stirred at 30^°C for 2 h. The solvent was removed in vacuo to give methyl 3,4,5,6-tetrahydropyrrolo[3,4-c]pyrrole-2(lH)-carboxylate TFA salt (210 mg, 100% yield), which was used directly for the next step without further purification. ESI-LCMS (m/z): 169.7 found for [M+l]⁺.

[00453] Step 3: methyl (R)-5-(2-(5-(3-((tert-butoxycarbonyl)(methyl)amino)-2-((tert- butyldimethylsilyl)oxy)propoxy)-2-chlorophenyl)-6-(3,5-dimethylisoxazol-4-yl)-5-methyl pyrimidin-4-yl)-3,4,5,6-tetrahydropyrrolo [3,4-c]pyrrole-2(lH)-carboxylate. A reaction pressure vessel was charged with a mixture of tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3- (4-chloro-3-(4- chloro-6-(3,5-dimethylisoxazol-4-yl)-5-methyl pyrimidin-2- yl)phenoxy)propyl(methyl) carbamate (150 mg, 0.23 mmol); methyl 3,4,5,6- tetrahydropyrrolo[3,4-c]pyrrole-2(lH)-carboxylate TFA salt, (or any other suitably substituted primary or secondary amine, 0.79 mmol), KI (30 mg, 0.18 mmol), triethylamine (2 mL).and n-BuOH (3 mL). The vessel was capped, placed in a microwave reactor and irradiated for 2h. at external temperature of 140 °C. After being cooled down to room temperature, 20 mL of water was added and the mixture was extracted with EtOAc (20 mL x 3). The combined organic layers were washed with water (30 mL) and brine (30 mL), dried over Na₂S0₄, filtered and concentrated. The residue was purified by preparative TLC

(MeOH/CH₂Cl₂ = 1/25) to give methyl (R)-5-(2-(5-(3-((tert-butoxycarbonyl)(methyl)amino)- 2-((tert-butyldimethylsilyl)oxy)propoxy)-2-chloro phenyl)-6-(3,5-dimethylisoxazol-4-yl)-5- methylpyrimidin-4-yl)-3 ,4,5 ,6-tetrahydropyrrolo[3 ,4-c]pyrrole-2( 1 H)-carboxylate (145 mg, 81% yield). ESI-LCMS (m/z): 783.4 found for [M+l]⁺.

[00454] Step 4: methyl (R)-5-(2-(2-chloro-5-(2-hydroxy-3-

(methylamino)propoxy)phenyl)-6-(3,5-dimethylisoxazol-4-yl)-5-methylpyrimidin-4-yl)- 3,4,5,6-tetrahydropyrrolo[3,4-c]pyrrole-2(lH)-carboxylate. A solution of methyl (R)-5-(2- (5-(3-((tert-butoxycarbonyl)(methyl)amino)-2-((tert-butyldimethylsilyl)oxy) propoxy)-2- chlorophenyl)-6-(3, 5 -dimethylisoxazol-4-yl)-5 -methyl pyrimidin-4-yl)-3,4,5,6- tetrahydropyrrolo[3,4-c]pyrrole-2(lH)-carboxylate (100 mg, 0.12 mmol) in 90% TFA (3 ml) was stirred at 30°C for 72 h. The solvent was then removed in vacuo and the residue was dissolved in MeOH (5 mL). Ammonia was added to adjust the pH to 8-9; the mixture was concentrated under vacuum and the residue was purified by preparative HPLC to give methyl (R)-5-(2-(2-chloro-5-(2-hydroxy-3-(methylamino) propoxy)phenyl)-6-(3,5-dimethylisoxazol- 4-yl)-5-methylpyrimidin-4-yl)-3,4,5,6-tetrahydro pyrrolo[3,4-c]pyrrole-2(lH)-carboxylate (45 mg, 62 % yield). ESI-LCMS: 569.1 found for [M+l]⁺; ¹HNMR (400 MHz, CD₃OD) δ ppm: 7.39 (d, J= 8.8 Hz, 1H), 7.27 (d, J= 2.8 Hz, 1H) 7.06-7.00 (m, 1H), 4.71 (br s, 4H), 4.25 (br s, 4H), 4.13-4.08 (m, 1H), 4.06-3.98 (m, 2H), 3.76 (s, 3H), 2.84-2.70 (m, 2H), 2.47 (s, 3H), 2.41 (s, 3H), 2.36 (s, 3H), 2.27 (s, 3H).

Example 10. Preparation of (R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6- (5-(2,2,2-trifluoroethyl)-l,3,4,5,6,7-hexahydro-2H-pyrrolo[3,4-c]pyridin-2-yl)pyrimidin- 2-yl) phenoxy)-3-(methylamino)propan-2-ol

[00455] Step 1: tert-butyl lH-pyrrolo[3,4-c]pyridine-2(3H)-carboxylate. A solution of 2,3-dihydro-lH-pyrrolo[3,4-c]pyridine dihydrochloride (1.0 g, 5.2 mmol) and Et N (1.1 g, 10.9 mmol) in DCM (50 mL) was treated with slow addition of Boc₂0 (1.2 g, 5.5 mmol). The mixture was stirred at room temperature for 2h, concentrated in rotary evaporator and the residue was purified by column chromatography over silicagel (petroleum ether/EtOAc = 1/1) to give tert-butyl lH-pyrrolo[3,4-c]pyridine-2(3H)-carboxylate (0.9 g, 78 % yield). ESI- LCMS (m/z): 221.1 found for [M+H]⁺.

[00456] Step 2: 2-(tert-butoxycarbonyl)-5-(2,2,2-trifluoroethyl)-2,3-dihydro-lH- pyrrolo[3,4-c]pyridin-5-ium trifluoromethanesulfonate. A solution of tert-butyl 1H- pyrrolo[3,4-c]pyridine-2(3H)-carboxylate (500 mg, 2.3 mmol) in MeCN (10 mL) was treated with 2,2,2-trifluoroethyl trifluoromethane sulfonate (1.1 g, 4.7 mmol) and the reaction mixture was stirred at external temperature of 80°C for 4 h. After being cooled down to room temperature, the mixture was concentrated to give 2-(tert- butoxycarbonyl)-5-(2,2,2- trifluoroethyl)-2,3-dihydro-lH-pyrrolo[3,4-c]pyridin-5-ium trifluoro methanesulfonate (0.7 g, crude), which was used directly without further purification. ESI-LCMS (m/z): 303.1 found for [M+H]⁺.

[00457] Step 3: tert-butyl 5-(2,2,2-trifluoroethyl)-4,5,6,7-tetrahydro-lH-pyrrolo[3,4- c]pyridine-2(3H)-carboxylate. A solution of 2-(tert-butoxycarbonyl)-5-(2,2,2- trifluoroethyl)-2,3-dihydro-lH- pyrrolo[3,4-c]pyridin-5-ium trifluoromethanesulfonate (700 mg, crude from step 2) in MeOH (100 mL) was treated with NaBH₃CN (302 mg, 4.8 mmol) and the reaction mixture was stirred at room temperature for 16h. and then concentrated under vacuum. The residue was dissolved in DCM (50 ml) and the solution was washed with water (50 ml). The organic layer was concentrated and the residue was purified by column chromatography over silicagel (petroleum ether/EtOAc = 1/3) to give tert-butyl 5- (2,2,2- trifluoroethyl)-4,5,6,7-tetrahydro-lH-pyrrolo[3,4-c]pyridine-2(3H)-carboxylate (white solid, 350 mg, 50% yield for two steps). ESI-LCMS (m/z): 307.0 found for [M+H]⁺.

[00458] Step 4: 5-(2,2,2-trifluoroethyl)-2,3,4,5,6,7-hexahydro-lH-pyrrolo[3,4- c]pyridine trifluoroacetate salt. A solution of tert-butyl 5-(2,2,2-trifluoroethyl)-4, 5,6,7- tetrahydro-lH-pyrrolo[3,4-c]pyridine-2(3H)-carboxylate (200 mg, 0.65 mmol) in TFA/DCM (v/v=l/3, 10 ml) was stirred at room temperature for 2h. and then the solvent was removed under vacuo to afford 5-(2,2,2-trifluoroethyl)-2,3,4,5,6,7- hexahydro-lH-pyrrolo[3,4- c]pyridine as trifluoroacetate salt, which was used directly without further purification.

Assumed quantitative yield. ESI-LCMS (m/z): 207.1 found for [M+H]⁺.

[00459] Step 5: tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4-chloro-3-(4- (3,5- dimethylisoxazol-4-yl)-5-methyl-6-(5-(2,2,2-trifluoroethyl)-4,5,6,7-tetrahydro-lH- pyrrolo[3,4-c]pyridin-2(3H)-yl)pyrimidin-2-yl)phenoxy)propyl(methyl) carbamate. A reaction pressure vessel was charged with a mixture of tert-butyl (R)-2-(tert-butyl

dimethylsilyloxy)-3-(4-chloro-3-(4-chloro-6-(3,5-dimethylisoxazol-4-yl)-5-methylpyrimidin- 2-yl)phenoxy)propyl(methyl)carbamate (364 mmol, 0.56 mmol); 5-(2,2,2-trifiuoroethyl)- 2,3,4,5,6,7-hexahydro-lH-pyrrolo[3,4-c] pyridine TFA salt, (or any other suitably substituted primary or secondary amine, 0.65 mmol), Et₃N (226 mg, 2.24 mmol) and n-BuOH (5 mL). The vessel was capped, placed in a microwave reactor and irradiated for 2h. at external temperature of 145 °C. The solvent was then concentrated in vacuo and the residue was purified by preparative TLC to afford tert-butyl (R)-2-(tert-butyldimethylsilyloxy)-3-(4- chloro-3-(4-(3,5-dimethylisoxazol- 4-yl)-5-methyl-6-(5-(2,2,2-trifluoroethyl)-4, 5,6,7- tetrahydro-lH-pyrrolo[3,4-c]pyridin-2(3H)-yl)pyrimidin-2- yl)phenoxy)propyl(methyl)carbamate (450 mg, 98 % yield). ESI-LCMS (m/z): 821.0 found for [M+H]⁺.

[00460] Step 6: (2R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6-(5-(2,2,2- trifluoroethyl)-4,5,6,7-tetrahydro-lH-pyrrolo[3,4-c]pyridin-2(3H)-yl)pyrimidin-2- yl)phenoxy)-3-(methylamino)propan-2-ol. A solution of tert-butyl (R)-2-(tert- butyldimethylsilyloxy)-3-(4-chloro-3-(4-(3,5-dimethyl- isoxazol-4-yl)-5-methyl-6-(5-(2,2,2- trifluoroethyl)-4,5,6,7-tetrahydro-lH-pyrrolo[3,4-c]pyridin-2(3H)-yl)pyrimidin-2- yl)phenoxy)propyl(methyl)carbamate (150 mg, 0.18 mmol) in TFA/water (20: 1 v/v, 10.5 mL) was stirred at room temperature for 16h. The mixture was concentrated under vacuum, the residue was dissolved in MeOH (10 ml), and the solution was adjusted to pH 7-8 with ammonia. The mixture was concentrated, and the residue was purified by preparative HPLC to give (2R)-l-(4-chloro-3-(4-(3,5-dimethylisoxazol-4-yl)-5-methyl-6-(5-(2,2,2- trifluoroethyl)-4,5,6,7-tetrahydro-lH-pyrrolo[3,4-c]pyridin-2(3H)-yl)pyrimidin-2-yl) phenoxy)-3 -(methyl amino)propan-2-ol (white solid, 38 mg, 35% yield). ESI-LCMS (m/z): 607.2 found for [M+H]⁺; ¹HNMR (400 MHz, CDC1₃) δ ppm: 7.26 (d, J= 8.8 Hz, 1H), 7.22(d, J= 3.2 Hz, 1H), 6.81 (dd, J= 8.8 and 3.2 Hz, 1H), 4.50 (br s, 4H), 4.02-3.97 (m, 1H), 3.91 (d, J = 5.2 Hz, 2H), 3.23 (s, 2H), 3.13-3.04 (m, 2H), 2.87-2.83 (m, 2H), 2.76-2.63 (m, 2H), 2.40 (s, 3H), 2.32 (s, 3H), 2.22 (s, 3H), 2.21 (s, 3H), 2.20-2.15 (m, 2H).

Biological Assays

General Materials

[00461] S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), bicine, Tween20, dimethylsulfoxide (DMSO), bovine skin gelatin (BSG), sodium butyrate and Tris(2- carboxyethyl)phosphine hydrochloride solution (TCEP) were purchased from Sigma- Aldrich at the highest level of purity possible. H-SAM was purchase from American Radiolabeled Chemicals with a specific activity of 80 Ci/mmol. 384-well streptavidin Flashplates were purchased from PerkinElmer.

Substrates

[00462] Peptide representative of human histone H3 residues 16-30 was synthesized with an N-terminal linker-affinity tag motif and a C-terminal amide cap by 21^st Century

Biochemicals. The peptide was purified by high-performance liquid chromatography (HPLC) to greater than 95% purity and confirmed by liquid chromatography mass spectrometry (LC- MS). The sequence was Biot-Ahx-PRKQLATKAARKSAP-amide and contained a monomethylated arginine at position 26 (SEQ ID NO.:l).

Molecular Biology

[00463] Human CARM1 (PRMT4) (NM 199141.1) transcript clone was amplified from an HEK 293 cDNA library, incorporating a flanking 5 ' sequence encoding a FLAG tag

(MDYKDDDDK) (SEQ ID NO.:2) fused directly to Ala 2 of CARM1 and 3' sequence encoding a hexa His sequence (EGHHHHHH) (SEQ ID NO.:3) fused directly to Ser 608. The gene sequence encoding isoforml containing a deletion of amino acids 539-561 was amplified subsequently and subcloned into pFastBacMam (Viva Biotech).

Protein Expression

[00464] Recombinant baculovirus were generated according to Bac-to-Bac kit instructions (Life Technologies). Protein over-expression was accomplished by infecting exponentially growing HEK 293F cell culture at 1.3X10⁶cell/ml with virus (MOI = 10) in the presence of 8 mM sodium butyrate. Infections were carried out at 37°C for 48 hours, harvested by centrifugation, and stored at -80°C for purification.

Protein Purification

[00465] Expressed full-length human Flag- and His-tagged CARMl protein was purified from cell paste by anti-flag M2 affinity chromatography with resin equilibrated with buffer containing 20 mM Tris, 150 mM NaCl, 5% glycerol, pH 7.8. Column was washed with 500 mM NaCl in buffer A and Flag-CARMl-His was eluted with 200 ug/ml FLAG peptide in buffer A. Pooled fractions were dialyzed in 20 mM Tris, 150 mM NaCl, 5% glycerol and 1 mM DTT, pH 7.8. The purity of recovered protein was 94.

Predicted Translations

[00466] Flag-CARM 1 -His (SEQ ID NO. : 4)

[00467] MDYKDDDDKAAAAAAVGPGAGGAGSAVPGGAGPCATVSVFPGARLLTI

GDANGEIQRHAEQQALRLEVRAGPDSAGIALYSHEDVCVFKCSVSRETECSRVGKQS

FIITLGCNSVLIQFATPNDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLS

QQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAV

EASTMAQHAEVLVKS NLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLES

YLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALR

GAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLV

HGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLI

ANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNT

GSTYNLSSGMAVAGMPTAYDLSSVIASGSSVGHNNLIPLGSSGAQGSGGGSTSAHYA

VNSQFTMGGPAISMASPMSIPTNTMHYGSEGHHHHHH

General Procedure for CARM1 Enzyme Assays on Peptide Substrates

[00468] The assays were all performed in a buffer consisting of 20 mM Bicine (pH=7.6), 1 mM TCEP, 0.005% BSG, and 0.002% Tween 20, prepared on the day of use. Compounds in 100%) DMSO (lul) were spotted into a polypropylene 384-well V-bottom plates (Greiner) using a Platemate Plus outfitted with a 384-channel head (Thermo Scientific). DMSO (lul) was added to Columns 11, 12, 23, 24, rows A-H for the maximum signal control and lul of SAH, a known product and inhibitor of CARMl, was added to columns 11, 12, 23, 24, rows I-P for the minimum signal control. A cocktail (40ul) containing the CARMl enzyme was added by Multidrop Combi (Thermo-Fisher). The compounds were allowed to incubate with CARMl for 30 min at room temperature, then a cocktail (lOul) containing H-SAM and peptide was added to initiate the reaction (final volume = 5 lul). The final concentrations of the components were as follows: CARMl was 0.25 nM, H-SAM was 30 nM, peptide was 250 nM, SAH in the minimum signal control wells was 1 mM, and the DMSO concentration was 2%o. The assays were stopped by the addition of non-radiolabeled SAM (lOul) to a final concentration of 300 uM, which dilutes the H-SAM to a level where its incorporation into the peptide substrate is no longer detectable. 50ul of the reaction in the 384-well

polypropylene plate was then transferred to a 384-well Flashplate and the biotinylated peptides were allowed to bind to the streptavidin surface for at least 1 hour before being washed once with 0.1%Tween20 in a Biotek ELx405 plate washer. The plates were then read in a PerkinElmer TopCount plate reader to measure the quantity of H-labeled peptide bound to the Flashplate surface, measured as disintegrations per minute (dpm) or alternatively, referred to as counts per minute (cpm).

% inhibition calculation

o/o ink = 100 - (^dVm_cmpd - dpm_min^ _χ ^

dpm_max - dpm_min )

where dpm = disintegrations per minute, cmpd = signal in assay well, and min and max are the respective minimum and maximum signal controls.

parameter IC50 fit

Bottom

where top and bottom are the normally allowed to float, but may be fixed at 100 or 0 respectively in a 3 -parameter fit. The Hill Coefficient normally allowed to float but may also be fixed at 1 in a 3 -parameter fit. Y is the % inhibition and X is the compound concentration.

RKO methylation assay

[00469] RKO adherent cells were purchased from ATCC (American Type Culture

Collection), Manassas, VA, USA. DMEM/Glutamax medium, penicillin-streptomycin, heat inactivated fetal bovine serum, 0.05% trypsin and D-PBS were purchased from Life

Technologies, Grand Island, NY, USA. Odyssey blocking buffer, 800CW goat anti-rabbit IgG (H+L) antibody, and Licor Odyssey infrared scanner were purchased from Licor Biosciences, Lincoln, NE, USA. Asymmetric di-methyl PABP1 antibody was purchased from Cell Signaling Technology, Danvers, MA, USA. Methanol was purchased from VWR, Franklin, MA, USA. 10% Tween 20 was purchased from KPL, Inc., Gaithersburg, Maryland, USA. Paraformaldehyde (PFA) was purchased from EM Sciences. DRAQ5 was purchased from Biostatus Limited, Leicestershire, UK.

[00470] RKO adherent cells were maintained in growth medium (DMEM/Glutamax medium supplemented with 10% v/v heat inactivated fetal bovine serum and 100 units/mL penicillin-streptomycin) and cultured at 37 °C under 5% C0₂.

[00471] Cell treatment, In Cell Western (ICW) for detection of asymmetric di-methyl PABPl and DNA content: RKO cells were seeded in assay medium at a concentration of 30,000 cells per mL to a poly-D-lysine coated 384 well culture plate (BD Biosciences 356697) with 50 μΐ, per well. Compound (100 nL) from a 96-well source plate was added directly to 384 well cell plate. Plates were incubated at 37°C, 5% C0₂ for 48 hours. After two days of incubation, plates were brought to room temperature outside of the incubator for ten minutes and blotted on paper towels to remove cell media. Cells were fixed for 20 minutes at room temperature by adding 50 ul of 8% PFA followed by aspiration of supernatant with the Biotek EL406 plate washer. Cells were then permeabilized by addition of 50 μΐ_^ of ice cold 100% methanol directly to each well and incubated for 30 min at room temperature. After 30 min, plates were transferred to a Biotek EL406 plate washer and washed 2 times with 100 μΐ, per well of wash buffer (IX PBS). Next 60 μΐ, per well of Odyssey blocking buffer (Odyssey Buffer with 0.1% Tween 20 (v/v)) were added to each plate and incubated 1 hour at room temperature. Blocking buffer was removed and 20 per well of primary antibody was added (asymmetric-methyl PABPl) diluted 1 :400 in Odyssey buffer with 0.1%> Tween 20 (v/v)) and plates were incubated overnight (16 hours) at 4°C. Plates were washed 5 times with 100 μΐ, per well of wash buffer. Next 20 μΐ, per well of secondary antibody was added (1 :800 800CW goat anti-rabbit IgG (H+L) antibody, 1 :2000 DRAQ5 in Odyssey buffer with 0.1 % Tween 20 (v/v)) and incubated for 1 hour at room temperature. The plates were washed 5 times with 100 μί per well wash buffer then 2 times with 100 μί per well of water. Plates were allowed to dry at room temperature then imaged on the Licor Odyssey machine which measures integrated intensity at 700nm and 800nm wavelengths. Both 700 and 800 channels were scanned.

[00472] Calculations. First, the ratio for each well was determined by:

[00473] Each plate included fourteen control wells of DMSO only treatment (minimum inhibition) as well as fourteen control wells for maximum inhibition treated with 20 μΜ of a reference compound. The average of the ratio values for each control type was calculated and used to determine the percent activation for each test well in the plate. Reference compound was serially diluted three-fold in DMSO for a total of nine test concentrations, beginning at 20 μΜ.

[00474] Percent inhibition was determined and IC₅₀ curves were generated using triplicate wells per concentration of compound.

Percent Inhibition = 100—

OTHER EMBODIMENTS

[00475] The foregoing has been a description of certain non-limiting embodiments of the invention. Those of ordinary skill in the art will appreciate that various changes and modifications to this description may be made without departing from the spirit or scope of the present invention, as defined in the following claims.

Claims

What is claimed is:

1. A compound of Formula (I) :

or pharmaceutically acceptable salt thereof;

wherein:

R¹ is hydrogen, -CHO, or unsubstituted Ci_₃alkyl;

R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, or halogen;

Ring A is of formula (A-i), (A- -iii):

wherein:

each instance of R^A1 and R^ is independently unsubstituted Ci_₃alkyl, Ci_ 3haloalkyl, or unsubstituted cyclopropyl;

R^ is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN; R^A4 is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, or -CN; and R^A5 is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl;

Ring B is any one of formula (i) to (xxviii):

(i) (ii) (iii)

402

(xxii) (xxiii) (xxiv)

(xxv) (xxvi) (xxvii)

(xxviii) (xxix) wherein:

q is 1 , 2, or 3 and w is 1 ; or q is 2 and w is 0 or 2;

x is 1 and y is 1 or 2;

n is 0, 1, or 2;

Li is -NH-, substituted or unsubstituted C₂alkylene, substituted or unsubstituted C₂alkenylene, or substituted or unsubstituted C₂alkynylene;

R^N1 is substituted or unsubstituted Ci_3alkyl, Ci_3haloalkyl substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^N1A, -C(=0)N(R^N1A)(R^N1B), -C(=0)OR^N1A, or -S(0)₂R^N1A; wherein:

R^N1A is substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl; R^N1B is hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6- membered heterocyclyl; or

unsubstituted 5- to 6-membered ring;

wherein:

R^B2 is hydrogen, halogen, -OR^B2A, substituted or unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, wherein R^B2A i_s substituted or unsubstituted Ci_₃alkyl or Ci_₃haloalkyl; or

R^B1 and R^B2 are joined to form a substituted or unsubstituted 4- to 6- membered heterocyclyl;

each instance of R is independently hydrogen, unsubstituted Ci_₃alkyl, or Ci_ haloalkyl, provided at least one instance of R B3 is hydrogen;

each instance of R^B4, R^B5, R^B6, and R^B7 is independently hydrogen, substituted or unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, halogen, -CN, -OR^B4B, -SR^B4B, - N(R^B4A)(R^B4B), substituted or unsubstituted C₃_₆ carbocyclyl, substituted or unsubstituted 4- to 6-membered heterocyclyl, -C(=0)R^B4A, -C(=0)N(R^B4A)(R^B4B), - C(=0)OR^B4A, -S(0)₂R^B4A, -OC(=0)R^B4A, -OC(=0)N(R^B4A)(R^B4B), -OC(=0)OR^B4A, NR^B4BC(=0)R^B4A, -NR^B4BC(=0)N(R^B4A)(R^B4B), or -NR^B4BC(=0)OR^B4A;

wherein:

R^B4A is substituted or unsubstituted Ci_3alkyl, Ci_3haloalkyl, substituted or unsubstituted C₃_₆ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl; and

and

wherein =^ represents a single or double bond; and

G

further wherein represents a single or double bond or G is -CH₂-;

and wherein:

wherein:

R is unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_6carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups; or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; and

R is hydrogen, unsubstituted Ci_₃alkyl, Ci_₃haloalkyl, C₃_₆carbocylyl unsubstituted or substituted with 1 or 2 R^D1 groups, or 4-6 membered heterocyclyl unsubstituted or substituted with 1 or 2 R^D1 groups; or

wherein:

each instance of R^D1 is independently halogen, -CN, -OR^D1A, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl, wherein R^D1A is hydrogen, unsubstituted Ci_₃alkyl, or Ci_₃haloalkyl.

2. The compound of claim 1, wherein R¹ is -CH₃.

3. The compound of claim 1, wherein R¹ is hydrogen.

4. The compound of claim 1, wherein R¹ is -CHO.

5. The compound of any one of claims 1-4, wherein R^2a is halogen and R^2b is hydrogen.

6. The compound of claim 5, wherein R^2a is -F or -CI.

7. The compound of any one of claims 1-4, wherein R^2a is Ci_3haloalkyl and R^2b is hydrogen.

8. The compound of claim 7, wherein R^2a is -CF₃.

9. The compound of any one of claims 1-4, wherein R^2a is halogen and R^2b is halogen.

10. The compound of claim 9, wherein R^2a is -CI and R^2b is -CI.

11. The compound of any one of claims 1-4, wherein each of R^2a and R^2b is hydrogen.

12. The compound of any one of claims 1-11, wherein R is -CH₃.

13. The compound of any one of claims 1-11, wherein R is -CI or -F.

14. The compound of claim 1, wherein each of R^2a is -CI and R³ is -CI.

15. The compound of claim 1, wherein R^2a is -F and R³ is -F.

16. The compound of claim 1, wherein R^2a is -CI and R³ is -CH₃.

17. The compound of claim 1, wherein R^2a is -F and R³ is -CH₃.

18. The compound of claim 1, wherein R^2a is -CF₃ and R³ is -CH₃.

The compound of any one of cl g A is of formula (A-i)

20. The compound of claim 19, wherein at least one of R and R is -CH₃ or -CH₂CH₃.

The compound of claim 19, wherein at least one of R and R is unsubstituted cyclopropyl.

22. The com ound of claim 19, wherein Rin A is:

The compound of any one of cl g A is of formula (A-ii)

24. The compound of claim 23, wherein at least one of R and R is halogen or -CN.

25. The compound of claim 23 or 24, wherein R is -CH₃ or -CH₂CH₃.

26. The compound of claim 23, wherein R is hydrogen.

The compound of claim 23, wherein R is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl R^A4 is halogen or -CN.

28. The compound of claim 23, wherein R^A4 is unsubstituted Ci_₃alkyl or Ci_₃haloalkyl and R^ is halogen or -CN. The compound of claim 23, wherein Ring A

31. The compound of claim 30, wherein R is -CH₃ or -CH₂CH .

32. The compound of claim 30, wherein R^ is halogen, unsubstituted Ci_₃alkyl, or Ci_ ₃haloalkyl.

33. The compound of claim 30, wherein Ring A is:

34. The compound of any one of the proceeding claims, wherein each instance of unsubstituted Ci_3alkyl is independently -CH₃, -CH₂CH₃, or -CH(CH₃)₂.

35. The compound of any one of the proceeding claims, wherein each instance of Ci_ ₃haloalkyl is independently -CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, - CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, - CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

36. The compound of any one of the proceeding claims, wherein each instance of substituted Ci_₃alkyl is independently Ci_₃alkyl substituted with 1, 2, or 3 R groups, as valency permits, selected from the group consisting of halogen, -CN, -O

, - N(R^C1A)(R^C1B), -C(=0)R^C1A, -C(=0)N(R^C1A)(R^C1B), -C(=0)OR^clA, -S(0)₂R^C1A, - OC(=0)R^clA, -OC(=0)N(R^clA)(R^clB), -OC(=0)OR^clA, -NR^CIBC(=0)R^B1A, - NR^CIBC(=0)N(R^C1A)(R^C1BB), and -NR^CIBC(=0)OR^clA.

37. The compound of claim 36, wherein each instance of substituted Ci_₃alkyl is independently Ci_₃alkyl substituted with 1 or 2 R groups selected from the group consisting of -CN, -OR^clB, -N(R^C1A)(R^C1B), -C(=0)N(R^C1A)(R^C1B), and -C(=0)OR^clA.

38. The compound of claim 37, wherein each instance of substituted Ci_₃alkyl is independently:

The compound of any one of the preceding claims, wherein each instance of B^1A)(R^B1B) is independently:

40. The compound of claim 39, wherein each instance of - N(R )(R ) is independently:

41. The compound of any one of the preceding claims, wherein each instance of^■ N(R^C1A)(R^C1B) is independentl

CIA. C1B

42. The compound of claim 41, wherein each instance of - N(R )(R ) is independently:

43. The compound of any one of the preceding claims wherein Ring B is of formula (iii), (v), (vi), (vii), (viii), (ix), (x), (xii), (xiii), (xxii), (xxvii), or (xxviii), and further wherein each instance of R^N1 is independently substituted or unsubstituted C_1-3alkyl, C_1-3haloalkyl, substituted or unsubstituted C₃ carbocyclyl, -C(=0)R^N1A, -C(=0)OR^N1A, -S(0)₂R^N1A, - C(=0)N(R^N1A)(R^N1B), substituted or unsubstituted 4- membered heterocyclyl, substituted or unsubstituted 5- membered heterocyclyl, or substituted or unsubstituted 6-membered heterocyclyl.

44. The compound of claim 43, wherein at least one instance of R is an unsubstituted Ci_₃alkyl of formula -CH₃, -CH₂CH₃, or -CH(CH₃)₂.

45. The compound of claim 43, wherein at least one instance of R is Ci_₃haloalkyl of formula -CF₃, -CC1₃, -CFC1₂, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, -CF₂CF₃, -CH₂CF₃, CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, -CF₂CF₂CF₃, -CH₂CF₂CF₃, - CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, or -CH(CH₃)CF₃.

46. The compound of claim 43, wherein at least one instance of R is substituted Ci_ ₃alkyl of formula:

47. The compound of claim 43, wherein at least one instance of R is substituted or unsubstituted C₃ carbocyclyl.

48. The compound of claim 47, wherein at least one instance of R^N1 is

49. The compound of claim 43, wherein at least one instance of R is substituted or unsubstituted 4- to 6-membered heterocyclyl comprising one oxygen ring heteroatom.

50. The compound of claim 49, wherein at least one instance of R^N1 is:

51. The compound of claim 43, wherein at least one instance of R is -C(=0)R , - C(=0)OR^N1A, or -S(0)₂R^N1A, wherein R^N1A is -CH₃, -CH₂CH₃, -CH₂CH₂CH₃, -CH(CH₃)₂, - CF₃, -CCI3, -CFCI2, -CF2CI, -CH₂F, -CHF₂, -CH2CI, CHC1₂, -CF₂CF₃, -CH₂CF₃, - CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, -CF₂CF₂CF₃, -CH₂CF₂CF₃, - CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, -CH(CH₃)CF₃. least one instance of R is -

st one instance of R

54. The compound of any one of the preceding claims,

wherein:

R^B1 is unsubstituted Ci_₃ alkyl, C_1-3haloalkyl, Ci_₃ alkyl substituted with - OR^clB, Ci_₃ alkyl substituted with -N(R^C1A)(R^C1B), Ci_₃alkyl substituted with -CN, Ci 3 alkyl substituted with -C(=0)N(R^C1A)(R^C1B), Ci_₃ alkyl substituted with - C(=0)OR^clA, -C(=0)N(R^B1A)(R^B1B), -OC(=0)OR^B1A, -N(R^B1A)(R^B1B), -OR^B1B, - SR^B1B, -S(0)₂R^B1A, -F, -CI, -CN, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4- to 6-membered heterocyclyl;

or

at least one instance of R^B4, R^B5, R^B6, and R^B7 is unsubstituted Ci_₃ alkyl, Ci_ 3haloalkyl, Ci_₃ alkyl substituted with -OR , Ci_₃ alkyl substituted with - N(R^C1A)(R^C1B), Ci_₃alkyl substituted with -CN, Ci_₃ alkyl substituted with - C(=0)N(R^C1A)(R^C1B), Ci_₃ alkyl substituted with -C(=0)OR^clA, - C(=0)N(R^B4A)(R^B4B), -OC(=0)OR^B4A, -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, -S(0)₂R^B4A, F, -CI, -CN, substituted or unsubstituted C₃ carbocyclyl, or substituted or

unsubstituted 4- to 6-membered heterocyclyl

55. The compound of claim 54,

wherein:

R^B1 is -CH₃, -CH₂CH₃, -CH₂CH₂CH₃, -CH(CH₃)₂, -CF₃, -CC1₃, -CFC1₂, -CF₂C1, - CH₂F, -CHF₂, -CH₂CI, CHC1₂, -CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH₂CC1₃, - CH₂CHC1₂, -CH₂CH₂CI, -CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, - CH(CH₃)CF₃,

at least one instance of R , R , R^B6, and R^B7 is -C¾, -CH₂CH₃, -CH₂CH₂CH₃ CH(CH₃)₂, -CF₃, -CCI3, -CFCI2, -CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, -CF₂CF CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, -CH2CCI3, -CH₂CHC1₂, -CH₂CH₂C1, -CF₂CF₂CF₃ CH₂CF₂CF₃, -CH₂CH₂CF₃, -CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, -CH(CH₃)CHF₂, -CH(CH₃)CF₃,

56. The compound of any one of the preceding claims, wherein R is hydrogen, -OR¹ -F, unsubstituted Ci_₃ alkyl, Ci_ haloalkyl, or Ci_ alkyl substituted with -0R^C1B.

57. The compound of any one of the preceding claims, wherein R is hydrogen or - provided at least one instance of R is hydrogen.

58. The compound of any one of the preceding claims, wherein each instance of R^B8 R^N2 is independently -CH₃, -CH₂CH₃, -CH₂CH₂CH₃, -CH(CH₃)₂, -CF₃, -CC1₃, -CFC1₂, CF₂C1, -CH₂F, -CHF₂, -CH₂C1, CHC1₂, -CF₂CF₃, -CH₂CF₃, -CH₂CHF₂, -CH₂CH₂F, - CH₂CC1₃, -CH₂CHC1₂, -CH₂CH₂C1, -CF₂CF₂CF₃, -CH₂CF₂CF₃, -CH₂CH₂CF₃, - CH₂CH₂CHF₂, - CH₂CH₂CH₂F, - CH₂CH₂CC1₃, - CH₂CH₂CHC1₂, - CH₂CH₂CH₂C1, - CH(CH₃)CHF₂, or -CH(CH₃)CF₃; or R^N2 and R^B8 are joined to form an unsubstituted 5- membered ring; or R^N2 and R^B8 are joined to form an unsubstituted 5-membered ring.

59. The compound of any one of the preceding claims wherein Ring B is of formula (i), (ii), or (xxvi), further wherein:

a. R^B1 is -N(R^B1A)(R^B1B), -OR^B1B, -SR^B1B, -S(0)₂R^B1A, -F, -CI, -CN, - OC(=0)OR^B1A, -C(=0)N(R^B1A)(R^B1B), and R^B2 is hydrogen; or b. R^B1 is -F and R^B2 is -F; or

c. R^B1 is -OR^B1B, -C(=0)N(R^B1A)(R^B1B), -CN , or Ci_₃ alkyl substituted with - OR^clB, Ci_₃ alkyl substituted with -N(R^C1A)(R^C1B), and R^B2 is substituted or unsubstituted Ci_₃ alkyl or Ci_₃haloalkyl; or

d. R^B1 is -OR^B1B and R^B2 is -OR^B2A, and each instance of R^B1B and R^B2A is

independently substituted or unsubstituted Ci_₃ alkyl or Ci_₃haloalkyl; or R^B1 is -OR^B1B and R^B2 is -OR^B2A, and R^B1B and R^B2A are joined to form a substituted or unsubstituted 5- to 6- membered heterocyclyl.

60. The compound of any one of the preceding claims, wherein Ring B is of formula (xiv), further wherein:

a. each instance of R^B5 and R^B7 is hydrogen; or

b. each instance of R^B5 and R^B7 is independently -OR^B4B; or

61. The compound of any one of the preceding claims, wherein Ring B is of formula (xv), further wherein:

a. each instance of R^B5, R^B6, and R^B7 is hydrogen; or

62. The compound of any one of the preceding claims, wherein Ring B is of formula (xvi), further wherein:

a. R^B4is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B6 and R^B7 are hydrogen; or

b. R^B6is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B5 and R^B7 are hydrogen; or

c. R^B7is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B5 and R^B6 are hydrogen; or d. R^B4and R^B6are-N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B7 is hydrogen.

63. The compound of any one of the preceding claims, wherein Ring B is of formula (xvii), further wherein:

a. R^B5is -N(R^B4A)(R^B4B), -OR^B4B, -SR^B4B, halogen, substituted or unsubstituted Ci_₃ alkyl, Ci_₃haloalkyl, substituted or unsubstituted C₃ carbocyclyl, or substituted or unsubstituted 4-6 membered heterocyclyl, and R^B6 is hydrogen; or

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 64, wherein Ring B is of formula:

66. The compound of claim 65, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 67, wherein Ring B is of formula:

The compound of claim 68, wh of formula:

70. The compound of any one of claims 1-58, wherein Ring B is of formula:

JD^N-^RNI (iii).

71. The compound of claim 70, wherein Ring B is of formula:

The compound of any one of claims 1-58 wherein Ring B is of formula:

The compound of claim 72, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 74, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The com ound of claim 76 wherein Ring B is of formula:

78. The compound of any one of claims 1-58, wherein Ring B is of formula:

79. The compound of claim 78, wherein R is hydrogen or -CH₃CH₃.

The compound of claim 79, wherein Ring B is of formula:

81. The compound of any one of claims 1-58, wherein Ring B is of formula:

82. The compound of claim 81 , wherein q is 1 , 2, or 3 and w is 1.

83. The compound of claim 81 , wherein q is 2 and w is 0 or 2.

84. The compound of claim 81 , wherein Ring B is of formula:

85. The compound of claim 84, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 86, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 88, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 90, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 92 wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

95. The compound of claim 94, wherein Ring B is of formula:

96. The compound of any one of claims 1-58, wherein Ring B is of formula:

424

425

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 100, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 102, wherein Rin B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

(xviii).

The compound of claim 104, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of laim 106, wherein Ring B is of formula:

The compound of any one of claims 1-58 wherein Ring B is of formula:

The compound of claim 108, wherein Ring B is of formula:

110. The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 110, wherein Rin B is of formula:

The compound of any one of claims 1-58 wherein Ring B is of formula:

113. The compound of claim 112, wherein x is 1 and y is 1.

114. The compound of claim 112 , wherein x is 1 and y is 2.

The compound of claim 112, wherein Ring B is of formula:

The compound of claim 112, wherein Rin B is of formula:

The com ound of claim 112, wherein Ring B is of formula:

The compound of claim 112, wherein Ring B is of formula:

The compound of any one of claims 1-58 wherein Ring B is of formula:

(xxiii).

The compound of claim 119, wherein Rin B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 121 wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

The compound of claim 124, wherein Ring B is of formula:

The compound of claim 124, wherein Ring B is of formula:

The compound of claim 124 wherein Rin B is of formula:

The compound of any one of claims 1-58, wherein Ring B is of formula:

(xxvii).

131. The compound of claim 130 , wherein q is 1 , 2, or 3 and w is 1.

132. The compound of claim 130, wherein q is 2 and w is 0 or 2.

The compound of claim 130, wherein Ring B is of formula:

Th compound of claim 130, wherein Ring B is of formula:

The compound of claim 130, wherein Ring B is of formula:

The com ound of claim 130, wherein Ring B is of formula:

The compound of claim 130, wherein Ring B is of formula:

138. The compound of claim 130, wherein Ring B is of formula:

139. The compound of any one of claims 1-58, wherein Ring B is of formula:

(xxviii).

140. The compound of claim 139, wherein Ring B is of formula:

The compound of an one of claims 1-58, wherein Ring B is of formula:

The compound of claim 141 wherein Ring B is of formula:

The compound of any one of claims 1-142, wherein the compound is of Formula:

harmaceutically acceptable salt thereof.

The compound of any one of claims 1-142, wherein the compound is of Formula:

or a pharmaceutically acceptable salt thereof.

145. The compound of any one of claims 1-142, wherein the compound is of Formula:

441

or a pharmaceutically acceptable salt thereof.

147. The compound of any one of claims 1-146, wherein the compound is selected from the group consisting of compounds depicted in Table 1 and pharmaceutically acceptable salts thereof.

148. A pharmaceutical composition comprising a compound of any one of claims 1-146 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.

149. A kit or packaged pharmaceutical comprising a compound of any any one of claims 1- 146 or a pharmaceutically acceptable salt thereof, and instructions for use thereof.

150. A method of treating a CARM1 -mediated disorder, comprising administering to a subject in need thereof an effective amount of a compound of any one of claims 1-146, or a pharmaceutically acceptable salt thereof.

151. The method of claim 150, wherein the disorder is a proliferative disorder.

152. The method of claim 151 , wherein the proliferative disorder is cancer.

153. The method of claim 152, wherein the cancer is associated with E2F1 upregulation.

154. The method of claim 152, wherein the cancer is associated with aberrant CARM1 activity.

155. The method of claim 152, wherein the cancer is breast cancer, prostate cancer, or colorectal cancer.

156. The method of claim 152, wherein the cancer is ERa-dependent breast cancer.

157. The method of claim 152, wherein the cancer is castration-resistant prostate cancer.

158. The method of claim 152, wherein the cancer is colorectal cancer associated with dysregulated WNT/ -catenin signaling.

159. The method of claim 150, wherein the disorder is a metabolic disorder.