WO2016201972A1 - 一种小分子多肽zy4及其应用 - Google Patents

一种小分子多肽zy4及其应用 Download PDF

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Publication number
WO2016201972A1
WO2016201972A1 PCT/CN2016/070739 CN2016070739W WO2016201972A1 WO 2016201972 A1 WO2016201972 A1 WO 2016201972A1 CN 2016070739 W CN2016070739 W CN 2016070739W WO 2016201972 A1 WO2016201972 A1 WO 2016201972A1
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small molecule
application
polypeptide
molecule polypeptide
antibacterial
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Ceased
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PCT/CN2016/070739
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English (en)
French (fr)
Inventor
赖仞
容明强
杜彦军
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Sichuan Hetai Synlight Biotech Ltd
Kunming Institute of Zoology of CAS
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Sichuan Hetai Synlight Biotech Ltd
Kunming Institute of Zoology of CAS
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Priority to JP2017565996A priority Critical patent/JP6546296B2/ja
Priority to EP16810713.4A priority patent/EP3309167B1/en
Priority to US15/580,694 priority patent/US10208088B1/en
Publication of WO2016201972A1 publication Critical patent/WO2016201972A1/zh
Anticipated expiration legal-status Critical
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/10—Peptides having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/10—Anti-acne agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • the invention belongs to the field of biomedical technology, and particularly relates to the application of the small molecule polypeptide ZY4 in the preparation of acne, anti-infective anti-tumor drugs and cosmetics.
  • Acne is a chronic inflammation of the hair follicle sebaceous glands. It is a common inflammatory skin disease in adolescence. It occurs in the cheeks, forehead, cheeks and nasolabial folds, followed by the chest, back and shoulders. Level, sebaceous gland secretion, P. acnes proliferation, keratinization abnormalities of hair follicle sebaceous gland ducts and inflammation. Adolescent skin surface lipid composition has changed, and male hormones increase, causing hyperkeratosis of sebaceous hair follicles.
  • Epithelial cells that are detached from the hair follicle wall are mixed with sebum, embolized in the hair follicle mouth to form acne, and then acted as propionic acid bacteria. In addition to bacterial infection, it causes inflammation, produces abscesses, and is extremely painful after suffering.
  • Tumor is a new organism in which a certain cell of a local tissue loses its normal regulation at the genetic level due to various carcinogenic factors, resulting in its clonal abnormal proliferation. According to the size of the tumor and its growth characteristics, it is divided into benign tumors and malignant tumors. Malignant tumors grow rapidly, often infiltrating into surrounding tissues during growth, with few envelopes on the surface, and often have systemic metastases. At present, the incidence of malignant tumors has been increasing year by year, and it is also among the top causes of death of various diseases, and thus it is a disease that is mainly controlled.
  • the antibacterial peptide is a biologically active small molecule polypeptide induced by the living body, and has a molecular weight of about 1000 to 7000 and consists of 10 to 60 amino acid residues.
  • Antimicrobial peptides are high-efficiency, spectral antibacterial peptide molecules that are rapidly produced by organisms when they are invaded by microorganisms to participate in the body's immune response. Antibacterial peptides are widely used as effective defense molecules in the body. At present, thousands of antimicrobial peptides have been identified from microorganisms, plants, insects, arthropods, amphibians, mammals and even humans.
  • Antibacterial peptides have complex antibacterial mechanisms, but most of them believe that the mechanism involves the cationic and hydrophobic properties of antimicrobial peptides and the action of negatively charged microbial membranes. When the antimicrobial peptides and bacterial cell membranes are in contact, the membrane permeability changes. Or forming a transmembrane pore on the bacterial cell membrane, which eventually causes the bacterial contents to leak out and die. Therefore, antibacterial peptides are much faster than traditional antibiotics in killing bacteria, and unlike antibiotics, which inhibit the growth of bacteria at low concentrations, the effects of antimicrobial peptides on bacteria are almost fatal.
  • antibacterial peptides are less likely to induce drug-resistant strains than traditional antibiotics; antibacterial spectrum light has effects on bacteria, fungi, viruses, protozoa and cancer cells.
  • Three families of antimicrobial peptides have been found in poultry, including cathelicidin, liver-expressed antimicrobial peptide (LEAP), and beta-defensin. These antibacterial peptides are crucial for poultry to resist bacterial and viral diseases, and mutations or deletions associated with these genes will have a significant impact on the ability of poultry to be resistant to microbial infections;

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Dermatology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Cosmetics (AREA)

Abstract

提供了一种小分子多肽ZY4，所述多肽的氨基酸序列为缬氨酸-半胱氨酸-赖氨酸-精氨酸-色氨酸-赖氨酸-赖氨酸-色氨酸-赖氨酸-精氨酸-赖氨酸-色氨酸-赖氨酸-赖氨酸-色氨酸-半胱氨酸-缬氨酰胺。还提供了所述多肽在制备用于抗感染、抗肿瘤和治疗痤疮的药物中的用途。

Description

一种小分子多肽ZY4及其应用 技术领域
本发明属于生物医学技术领域，具体涉及小分子多肽ZY4在制备治疗痤疮、抗感染抗肿瘤药物及化妆品中的应用。
背景技术
痤疮是一种毛囊性皮脂腺的慢性炎症，是青少年时期常见的炎性皮肤病，好发生于面颊、额部、颊部和鼻唇沟，其次是胸部、背部和肩部，其发病主要与性激素水平、皮脂腺大量分泌、痤疮丙酸杆菌增殖、毛囊皮脂腺导管的角化异常及炎症等因素相关。青春期的皮肤表面脂质成分有所改变，雄性激素增多，造成皮脂毛囊导管角化过度，毛囊壁上脱落的上皮细胞与皮脂混合，栓塞在毛囊口内，形成粉刺，再受丙酸菌等作用，加上细菌感染，引起炎症，产生脓疮，患后异常痛苦。
肿瘤是机体在各种致癌因素作用下，局部组织的某一个细胞在基因水平上失去对其生长的正常调控，导致其克隆性异常增生而形成的新生物。根据肿瘤对人体危害的大小及其生长特性而分为良性肿瘤和恶性肿瘤两类。恶性肿瘤生长迅速，生长时常向周围组织浸润，表面几无包膜，常有全身转移。目前，恶性肿瘤的发病率有逐年升高的趋势，在各种疾病的死亡原因中也占前几位，因而是重点防治的一种疾病。
抗菌肽是生物体内经诱导产生的一种具有生物活性的小分子多肽，分子量在1000～7000左右，由10～60个氨基酸残基组成。抗菌肽是当生物受到微生物侵入后机体迅速产生的高效、光谱的抗菌肽类分子，来参与机体的免疫反应。抗菌肽作为机体有效的防御分子是广泛存在的，目前已从微生物、植物、昆虫、节肢动物、两栖动物、哺乳动物甚至人体内鉴定出上千种抗菌肽。抗菌肽抗菌机理复杂，但大多数理论都认为其机制涉及到抗菌肽的阳离子性和疏水性与带负电荷的微生物胞膜的作用，抗菌肽和细菌的细胞膜接触后，引起膜通透性改变，或在细菌细胞膜上形成跨膜的孔洞，最后导致细菌内容物外泄而死亡。因此，抗菌肽在杀灭细菌的速度上要远远高于传统的抗生素，且不像抗生素在低浓度下抑制细菌的生长，抗菌肽对细菌的作用几乎都是致死性的。研究表明：抗菌肽与传统抗生素相比，不易诱导耐药菌株的产生；抗菌谱光，对细菌、真菌、病毒、原虫及癌细胞均有作用。已经在家禽体内发现了3个家族的抗菌肽，包括cathelicidin，liver-expressed antimicrobial peptide(LEAP)，andβ-defensin。这些抗菌肽对于家禽抵抗细菌性、病毒性疾病具有至关重要的作用，相关这些基因的突变或者缺失对于家禽抗微生物感染的能力将有显著的影响；这些抗菌肽除了具

Claims (1)

  1. Figure PCTCN2016070739-appb-100001
PCT/CN2016/070739 2015-06-15 2016-01-12 一种小分子多肽zy4及其应用 Ceased WO2016201972A1 (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2017565996A JP6546296B2 (ja) 2015-06-15 2016-01-12 低分子ポリペプチドｚｙ４及びその使用
EP16810713.4A EP3309167B1 (en) 2015-06-15 2016-01-12 Small-molecule polypeptide zy4 and application thereof
US15/580,694 US10208088B1 (en) 2015-06-15 2016-01-12 Low molecular polypeptide ZY4 and applications thereof

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CN201510331263.5A CN104974228B (zh) 2015-06-15 2015-06-15 一种小分子多肽zy4及其应用
CN201510331263.5 2015-06-15

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Cited By (1)

* Cited by examiner, † Cited by third party
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CN115974970A (zh) * 2022-11-24 2023-04-18 山东理工大学 一种具有广谱抗菌性能的短链抗菌肽及其应用

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CN104974228B (zh) 2015-06-15 2017-10-17 四川合泰新光生物科技有限公司 一种小分子多肽zy4及其应用
CN107021999B (zh) * 2016-02-02 2021-03-09 香港中文大学深圳研究院 一种抗念珠菌的多肽及其用途、抗念珠菌的药物
CN110627870B (zh) * 2019-09-20 2022-10-21 成都佩德生物医药有限公司 一种小分子多肽rk12及其应用
CN113307855B (zh) 2021-06-25 2023-08-01 厦门大学 拟穴青蟹抗菌多肽Spampcin56-86及其应用
CN114681588B (zh) * 2022-02-17 2023-06-06 中山大学 多肽en-9在制备治疗痤疮的产品中的应用
CN116063390B (zh) * 2022-09-07 2023-11-24 东北农业大学 一种抗菌肽vck-17及其联合香茅醛做为抗菌药物的应用

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CN102924574A (zh) * 2012-10-26 2013-02-13 苏州康尔生物医药有限公司 抗菌肽lz1和该抗菌肽在制备抗菌药物中的用途
CN103275190A (zh) * 2013-06-14 2013-09-04 四川科伦新光生物科技开发有限公司 一种小分子多肽zy13及其应用
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115974970A (zh) * 2022-11-24 2023-04-18 山东理工大学 一种具有广谱抗菌性能的短链抗菌肽及其应用

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JP2018521041A (ja) 2018-08-02
US10208088B1 (en) 2019-02-19
CN104974228A (zh) 2015-10-14
EP3309167A1 (en) 2018-04-18
CN104974228B (zh) 2017-10-17
EP3309167B1 (en) 2019-11-27
JP6546296B2 (ja) 2019-07-17
EP3309167A4 (en) 2018-12-19

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