WO2017152146A2 - Compositions and methods for inducing hiv-1 antibodies - Google Patents

Abstract

In certain aspects the invention provides HIV-1 immunogens, including envelopes (CH0848) and selections therefrom, and methods for swarm immunizations using combinations of HIV- 1 envelopes.

Description

[0001] This application claims the benefit of and priority to U.S. Provisional Application No. 62/303,273 filed March 3, 2016 and U.S. Provisional Application No. 62/403,649 filed October 3, 2016 the contents of each of which are hereby incorporated by reference in their entireties.

[0002] The United States government has certain rights in this invention pursuant to Contract No, DE-AC52-06NA25396 between the United States Department of Energy and Los Alamos National Security, LLC for the operation of Los Alamos National Laboratory.

TECHNICAL FIELD

[0003] The present invention relates, in general, to human immunodeficiency virus (HIV), and, in particular, to HIV-1 immunogenic compositions their methods of making and their use in vaccination regimens.

BACKGROUND

[0004] Development of an effective vaccine for prevention of HTV-1 infection is a global priority. To provide protection, an HIV-1 vaccine should induce broadly neutralizing antibodies (bnAbs). One class of bnAbs among antibodies isolated from infected individuals targets the glycan-polypeptide at the base of the envelope third variable loop (V3). However, BnAbs have not been successfully induced by vaccine constructs thus far.

SUMMARY

[0005] The invention provides compositions comprising V3 antibody immunogens and methods for inducing antibodies to the V3 HIV-1 envelope region.

[0006] In one aspect the invention provides selection of immunogens which are used to induce V3 antibodies. In some embodiments, the immunogens include a homogeneous minimal immunogen with high mannose giycans reflective of a native Env V3-giycan bnAb epitope, (Man9-V3). In some embodiments, the immunogens include a homogeneous minimal immunogen without giycans.

[0007] V3-glycan bnAbs bound to Man9-V3 glycopeptide and native-like gpl40 trimers with similar affinities. Both fluorophore-labeled Mang-V3 or native-like trimers similarly bound to bn Ab memory B cells, and by flow sorting isolated members of a bnAb clonal lineage from an HIV-1 -infected individual. The glycopeptide of Figure 38A-E bound the germline of a V3-glycan bnAb lineage. Thus, a Mans-V3 glycopeptide mimics a HIV-1 V3-giycan bnAb epitope and is a candidate immunogen to initiate V3-glycan bnAb lineage maturation.

[0008] In some embodiments the compositions comprise immunologically and

pharmaceutically acceptable carriers and/or excipients.

[0009] In another aspect the invention provides a method of inducing an immune response in a subject comprising administering a combination of immunognes comprising V3-peptide and/or glycopeptide, wherein the peptide binds to a IJCA of a V3 glycan antibody, HIV-1 envelope CH848.0949.10.17; CH848.0836.10.31; CH848.0358.80.06; CH848.1432.5.41; CH848.0526.25.02 in any suitable form or any combination thereof as a prime and/or boost in an amount sufficient to induce an immune response, wherein the envelope is administered as a polypeptide or a nucleic acid encoding the same.

[0010] In certain embodiments, the compositions contemplate nucleic acid, as DNA and/or RNA, or proteins immunogens either alone or in any combination. In certain embodiments, the methods contemplate genetic, as DNA and/or RNA, immunization either alone or in combination with envelope protein(s).

[0011] In certain embodiments the nucleic acid encoding an envelope is operably linked to a promoter inserted an expression vector. In certain aspects the compositions comprise a suitable carrier. In certain aspects the compositions comprise a suitable adjuvant.

[0012] In certain embodiments the induced immune response includes induction of antibodies, including but not limited to autologous and/or cross-reactive (broadly) neutralizing antibodies against HIV-1 envelope. Various assays that analyze whether an immunogenic composition induces an immune response, and the type of antibodies induced are known in the art and are also described herein.

[0013] In certain aspects the invention provides an expression vector comprising any of the nucleic acid sequences of the invention, wherein the nucleic acid is operably linked to a promoter. In certain aspects the invention provides an expression vector comprising a nucleic acid sequence encoding any of the polypeptides of the invention, wherein the nucleic acid is operably linked to a promoter. In certain embodiments, the nucleic acids are codon optimized for expression in a mammalian cell, in vivo or in vitro. In certain aspects the invention provides nucleic acids comprising any one of the nucleic acid sequences of invention. In certain aspects the invention provides nucleic acids consisting essentially of any one of the nucleic acid sequences of invention. In certain aspects the invention provides nucleic acids consisting of any one of the nucleic acid sequences of invention. In certain embodiments the nucleic acid of the invention, is operably linked to a promoter and is inserted in an expression vector. In certain aspects the invention provides an immunogenic composition comprising the expression vector.

[0014] In certain aspects the invention provides a composition comprising at least one of the nucleic acid sequences of the invention. In certain aspects the invention provides a composition comprising any one of the nucleic acid sequences of invention. In certain aspects the invention provides a composition comprising at least one nucleic acid sequence encoding any one of the polypeptides of the invention,

[0015] The envelope used in the compositions and methods of the invention can be in any suitable form: a gpl60, gpl50, gpl45, any suitable form of a trimer, for example but not limited to SOSIP trimers, gpl40 (including but not limited to gpMGC, gpl40CF, gpl40CFI), gpl20, gp41 , N-terminal deletion variants (e.g. delta 11 deletions) as described herein, cleavage resistant variants, or codon optimized sequences thereof.

[0016] The polypeptide contemplated by the invention can be a polypeptide comprising any one of the polypeptides described herein. The polypeptide contemplated by the invention can be a polypeptide consisting essentially of any one of the polypeptides described herein. The polypeptide contemplated by the invention can be a polypeptide consisting of any one of the polypeptides described herein. In certain embodiments, the polypeptide is recombinantly produced. In certain embodiments, the polypeptides and nucleic acids of the invention are suitable for use as an immunogen, for example to be administered in a human subject.

[0017] In certain embodiments the envelope is any of the forms of HIV- 1 envelope. In certain embodiments the envelope is gp!20, gpl40, gp!45 (i.e. with a transmembrane), gpl50, optionally as a trimer. In certain embodiments the trimer is a chimeric SOSIP timer. See WO2016/037154 incorporated by reference in its entirety. In certain embodiments, envelope trimers are purified from cellular recombinant fractions by antibody binding and reconstituted in lipid comprising formulations. See for example WO2015/127108 titled "Trimeric HIV-1 envelopes and uses thereof" which content is herein incorporated by- reference in its entirety. In certain embodiments, the envelope is in a liposome and transmembrane with a cytoplasmic tail in a liposome. In certain embodiments, the nucleic acid comprises a nucleic acid sequence which encode a gpl20, gpl40 (including but not limited to gpl40C, gpl40CF, gpMOCFI), gpl45, gpl50 or gpl60.

[0018] The envelope used in the compositions and methods of the invention can be a gpl60, gpl50, gpl45, gp l 40, gp!20, gp41, N-terminal deletion variants as described herein, cleavage resistant variants as described herein, or codon optimized sequences thereof. In certain embodiments the composition comprises envelopes as trimers. In certain embodiments, envelope proteins are mutimerized, for example trimers are attached to a particl e such that multiple copies of the trimer are attached and the multimerized envelope is prepared and formulated for immunization in a human. In certain embodiments, the compositions comprise envelopes, including but not limited to trimers as particulate, high- density array on liposomes or other particles, for example but not limited to nanoparticles. In some embodiments, the trimers are in a well ordered, near native like or closed conformation. In some embodiments the trimer compositions comprise a homogenous mix of native like trimers. In some embodiments the trimer compositions comprise at least 85%, 90%, 95% native like trimers.

[0019] In certain embodiments, where the nucleic acids are operably linked to a promoter and inserted in a vector, the vectors is any suitable vector. Non-limiting examples, include, the VSV, replicating rAdenovirus type 4, MVA, Chimp adenovirus vectors, pox vectors, and the like. In certain embodiments, the nucleic acids are administered in NanoTaxi block polymer nanospheres. In certain embodiments, the composition and methods comprise an adjuvant. Non-limiting examples include, AS01 B, AS01 E, Gla/SE, alum, Poly I poly C (in any form, including but not limited to PolylC/long chain (LC)), TLR agonists, TLR7/8 and 9 agonists, or a combination of TLR 7/8 and TLR9 agonists (see Moody et al. (2014) J. Virol. March 2014 vol. 88 no. 6 3329-3339) , or any other adjuvant. Non-limiting examples of TLR7/8 agonist include TLR7/8 ligands, Gardiquimod, Imiquimod and R848 (resiquimod). A non- limiting embodiment of a combination of TLR7/8 and TLR9 agonist comprises R848 and oCpG in STS (see Moody et al. (2014) J. Virol. March 2014 vol. 88 no. 6 3329-3339).

[0020] In certain aspects, the invention provides a kit comprising a combination/selection of immunogens, for example but not limited to immunogens in Figure 37A-D, and Example 3. In some embodiments the selection of immunogens is selection F, selection G, or selection H. In some embodiments the kit comprises instructions on how to carry out the immunization regimen. In some embodiments the kit comprises instructions on administration of the selection of immunogens as a prime or boost as part of a prime/boost immunization regimen.

[0021] In certain aspects the invention provides a recombinant HIV-1 envelope polypeptide, wherein the polypeptide comprises the amino acid sequence of any one of the envel opes designs in Table 1, Table 3 or Example 3. In certain embodiments the envelope is engineered with modifications so as to improve its binding to the DH270UCA antibody. In certain embodiments, the engineered envelope is based on the sequence of HI V-1 envelope

CH848.0949.10.17. In certain embodiments, the protein does not include the signal peptide. In certain aspects the invention provides a recombinant HIV-1 envelope polypeptide from Table 1, Table 3 or Example 3 wherein the polypeptide is non-naturally occurring and designed to form a soluble trimer. In certain embodiments, the protein does not include the signal peptide. In certain aspects the invention provides a nucleic acid encoding any one of the polypeptides of the invention. In certain embodiments, the nucleic acids could be formulated in any suitable way for immunogenic delivery of nucleic acids.

[0022] In certain aspects the invention provides an immunogenic composition comprising the recombinant HIV-1 envelope polypeptides of the invention and a carrier. In certain aspects the invention provides an immunogenic composition comprising the nucleic acid of the invention and a carrier. The compositions could comprise an adjuvant,

[0023] In certain aspects the invention provides methods of inducing an immune response in a subject comprising administering a composition comprising an HIV-1 envelope

polypeptide(s) in an amount sufficient to induce an immune response from one or more of the following groups:

[0024] (a) V3 peptide in any suitable form such aglycone, glycosylated, multimerized, carrying T cell epitopes, etc., envelope polypeptide(s) designed to bind DH270UCA (Table 1,

Table 3, Ex. 3), or any combination thereof as a prime;

[0025] (b) envelope polypeptide(s) CH848.0949.10.17, CH848.0836.10.31,

CH848.0358, 80.06; CH848.1432,5.41 , CH848.0526.25.02 (Figure 37A), or any combination thereof;

[0026] (c) envelope polypeptide CH0848.3.dl651. 10.07;

[0027] and wherein the administration step can alternatively, or in addition, comprise administering any suitable form of a nucleic acid(s) encoding an HIV-1 envelope

polypeptide(s) in an amount sufficient to induce an immune response from one or more of the following groups:

[0028] (a) envelope polypeptide(s) designed to bind DH270UCA (Table 1 , Table 3, Ex. 3), or any combination thereof as a prime;

[0029] (b) envelope polypeptide(s) CH848.0949.10.17, CH848.0836.10.31 ,

CH848.0358.80.06; CH848.1432.5.41 ; CH848.0526.25.02 (Figure 37A), or any combination thereof;

[0030] (c) envelope polypeptide CH0848.3x11651 .10.07.

[0031] In certain embodiments, the first boost administered after the prime comprises HIV-1 envelope polypeptide CH848.0949.10. 17 in any suitable form.

[0032] In certain embodiments, the nucleic acid encodes a gpl20 envelope, gpl20Dl 1 envelope, a gpl 40 envelope (gpMOC, gpl40CF, gpl40CFI) as soluble or stabilized protomer of a SOSIP trimer, a gp 145 envelope, a gpl50 envelope, or a transmembrane bound envelope. In certain embodiments, the polypeptide is gpl20 envelope, gpl20Dl 1 envelope, a gpl40 envelope (gpl4()C, gpl4()CF, gpl40CFI) as soluble or stabilized protomer of a SOSIP trimer, a gpl45 envelope, a gpl50 envelope, or a transmembrane bound envelope. In certain embodiments, the methods further comprise administering an agent which modulates host immune tolerance. In certain embodiments, the immunogen of the invention is multimerized in a liposome or nanoparticle. In certain embodiment, the methods further comprise administering one or more additional HIV-1 immunogens to induce a T cell response.

[0033] In certain aspects the invention provides a kit comprising a combination/selection of immunogens of Selection I (V3 peptide in any suitable form such aglycone, glycosylated, multimerized, carrying T cell epitopes, etc.); recombinant HIV- 1 envelopes

CH848.0949.10.17; CH848.0358.80.06; CH848.1432.5,41; CH848.0526.25.02), and optionally envelope polypeptide CH0848.3.dl651.10.0, and/or a nucleic acid encoding the same in any suitable form. A kit comprising a combination/selection of immunogens comprising any suitable envelope design which binds to the DH270UCA; recombinant HIV-1 envelopes CH848.0949.10. 17; CH848.0358, 80.06; CH848.1432,5.41 , CH848.0526.25.02), and optionally envelope polypeptide (Ί 10848. d 165 i , 10,0. and/or a nucleic acid encoding the same in any suitable form. The envelope and/or nucleic acid in the kits of the invention could be in any suitable form. The V3 peptide in the kits of the invention could be of SEQ ID NO: 1. In some embodiments the peptide is glycosylated. In some embodiments, the peptide is not glycosylated. In some embodiments the kit comprises an adjuvant. In some embodiment the kit comprises instructions on how to cany out the immunization regiment: the immunogen could be administered sequentially or additively.

[0034] In certain aspects the invention provides a recombinant CH848 envelope protein designed to form a soluble trimer, wherein the CH848 envelope protein comprises the sequence of any one of the envelopes or designs in Tables 1, 3 and Ex, 3, Figures 39 A, 40 A or 41 A. In certain embodiments, the protein does not include the signal peptide.

[0035] In certain aspects the invention provides an immunogenic composition comprising the recombinant HIV-1 envelope (Ί 1848,0949. 10 1 7. CH848.0836.10,31 , CH848.0358.80.06, CH848.1432.5.41; (Ί 1848.0526.25.02 in any suitable form or a nucleic acid encoding the same. In certain embodiments the recombinant envelope comprises the sequence of the CHIM.6R.SOSIP.664V4.1 design. In certain embodiments the recombinant envelope comprises the sequence any other envelope form (See e.g. Figures 39-41; other forms such as gpl 40C, gpl 40CF, gpl40CFI). The invention also provides compositions comprising suitable form of an HIV-1 envelope polypeptide or any suitable form of a nucleic acid encoding HIV-1 envelope from the selections of envelopes listed in Tables 1, 3 and Ex, 3, Figures 39 A, 40 A or 41 A, or any combination thereof.

[0036] In certain aspects the invention provides a kit comprising a combination/selection of immunogens described in Tables 1, 3 and Ex, 3, and instructions for which immunogen are administered as a prime and which immunogens are administered as a boost. In some embodiments the kit of Selection I (V3 peptide in any suitable form such aglycone, glycosylated, multimerized, carrying T cell epitopes, etc.; recombinant HIV-1 envelopes CH848.0949.10. 17; CH848.0358, 80.06; CH848.1432,5.41 , CH848.0526.25.02) and/or a nucleic acid encoding the same. The envelope and/or nucleic acid in the kits of the invention could be in any suitable form. The V3 peptide in the kits of the invention could be of SEQ ID NO: 1. In some embodiments the peptide is glycosylated. In some embodiments, the peptide is not glycosylated. In some embodiments the kit comprises an adjuvant. In some embodiment the kit comprises instructions on how to carry out the immunization regiment: the immunogen could be administered sequentially or additiveiy.

[0037] In some aspects the invention provides a recombinant cell, a clonal population of ceils, or a pool of cells comprising a nucleic acid encoding any one of the envelope proteins or immunogens of the invention,

[0038] A recombinant HIV-1 Envelope ectodomain trimer, comprising three gp!20-gp41 protomers comprising a gpl20 polypeptide and a gp41 ectodomain, wherein each protomer is the same and comprises portions from envelope BG505 HIV-1 strain and gpl20 polypeptide portions from a CH0848 HIV-1 strain and stabilizing mutations A316W and E64K, wherein the trimer is stabilized in a prefusion mature closed conformation, and wherein the trimer does not comprise non-natural disulfide bond between cysteine substitutions at positions 201 and 433 of the HXB2 reference sequence. In some embodiments, the amino acid sequence of one monomer of trimer. In some embodiments, the trimer is immunogenic. In some embodiments the trimer binds to any one of the antibodies PGT145, PGT151 , CH103UCA, CH I 03, VRCOl, PGT128, or any combination thereof. In some embodiments the trimer does not bind to antibody 19B and/or 17B.

[0039] In certain embodiments the compositions comprising trimers are immunogenic. In certain aspects, the invention provides a pharmaceutical composition comprising any one of the recombinant trimers of the invention. In certain embodiments the compositions comprising trimers are immunogenic. The percent trimer in such immunogenic compositions could vary. In some embodiments the composition comprises 70%, 71 %, 72%, 73%, 74%,75%, 76%, 77%, 78%, 79%, 80%, 81 %, 82%, 83%, 84%, 85%, 86%, 87%, 89%, 90%, 91 %, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% stabilized ί rimer.

BRIEF DESCRIPTION OF THE DRAWINGS

[0040] The patent or application file contains at least one drawing executed in color. To conform to the requirements for PCT patent applications, many of the figures presented herein are black and white representations of images originally created in color.

[0041 j Figures 1 A-B. DH270 lineage with time of appearance and neutralization by selected members. (A) Phylogenetic relationship of 6 mAbs and 93 NGS VHDJH sequence reads in the DH270 clonal lineage. External nodes (filled circles) represent VHDJH nucleotide sequences of either antibodies retrieved from cultured and sorted memory B cells (labeled) or a curated dataset of NGS VHDJH rearrangement reads (unlabeled). Coloring is by time of isolation. Samples from week 1 1, 19, 64, 1 1 1 , 160, 186 and 240 were tested and time-points from which no NGS reads within the lineage were retrieved are reported in Figures 30A-C.

Internal nodes (open circles) represent inferred ancestral intermediate sequences. Units for branch-length estimates are nucleotide substitution per site, (B) Neutralization dendrograms display single mAb neutralization of a genetically diverse panel of 207 HIV-1 isolates. Coloring is by ICso. See also Fig. 33.

[0042] Figures 2A-D. Heterologous breadth in the DH270 lineage. (A) Neutralizing activity of DH270. I, DH270.5 and DH270.6 bnAbs (columns) for 207 tier 2 heterologous viruses (rows). Coloring is by neutralization IC?o (ug/ml). The first column displays presence of a PNG site at position 332 (blue), N334 (orange) or at neither one (black). The second column indicates the clade of each individual HIV- 1 strain and is color coded as indicated: clade A: green; clade B : blue; clade C : yellow: clade D: purple; CRFOI : pink; clade G: cyan; others: gray. See Fig. 33 , (B). Heterologous neutralization of all Di 1270 lineage antibodies for a 24- virus panel. Color coding for presence of PNG sites, clade and ICso is the same of panel A. See Figures 7A-D and Figures 34-35, (C) Co-variation between VH mutation frequencies (x- axis), neutralization breadth (y-axis, top panels) and potency (y-axis, bottom panels) of individual antibodies against viruses with a PNG site at position N332 from the larger (left) and smaller (right) pseudovirus panels. (D) Correlation between viral VI loop length and DH270 lineage antibody neutralization. Top panel: neutralization of 17 viruses (with N332 and sensitive to at least one DH270 lineage antibody) by selected DH270 lineage antibodies from UCA to mature bnAbs (x-axis). Viruses are identified by their respective VI loop lengths (y-axis); for each virus, neutralization sensitivity is indicated by an open circle and resistance by a solid circle. The p-value is a Wilcoxon rank sum comparison of VI length distributions between sensitive and resistant viruses. Bottom panel: regression lines (ICso for neutralization vs. VI loop length) for DH270. and DH270.6, with a p-value based on Kendall's tau.

[0043] Figures 3A-E. A single disfavored mutation early during DH270 clonal development conferred neutralizing activity to the V3 glycan bnAb DH270 precursor antibodies. (A) Nucleotide (nt) alignment of DH270.IA4 and DH272 to VH1-2*02 sequence at the four VH positions that mutated from DH270.UCA to DH270.IA4. The mutated codons are highlighted in yellow. AID hotspots are indicated by red lines (solid: canonical; dashed: non-canonical); AID cold spots by blue lines (solid: canonical; dashed: non-canonical) (20). At position 169, DH270.IA4 retained positional conformity with DH272 but not identity conformity (red boxes), (B) Sequence logo plot of aa mutated from germline (top) in NGS reads of the DH270 (middle) and DH272 (bottom) lineages at weeks 186 and 111 post-transmission, respectively. Red asterisks indicate aa mutated in DH270.IA4. The black arrow indicates lack of identity conformity between the two lineages at aa position 57. (C) Sequence logo plot of nucleotide mutations (position 165-173) in the DFI270 and DH272 lineages at weeks 86 and 111 post-transmission, respectively. The arrow indicates position 169. (D) Effect of reversion mutations on DH270.IA4 neutralization. Coloring is by ICso. (E) Effect of G57R mutation on DH270.UCA autologous (top) and heterologous (bottom) neutralizing activity.

[0044] Figures 4A-C. Cooperation among 1)1 1270. DH272 and DH475 N332 dependent V3 glycan nAb lineages. (A) Neutralizing activity of DH272, DH475 and DH270 lineage antibodies (columns) against 90 autologous viruses isolated from GT848 over time (rows). Neutralization potency (ICso) is shown as indicated in the bar. For each pseudovirus, presence of an N332 PNG site and VI loop length are indicated on the right. Also see Figs. 34-35. (B, C) Susceptibility to DH270.1 and to (B) DH475 or (C) DH272 of autologous viruses bearing selected immunotype-specific mutations.

[0045] Figures 5A-H. Fab/scFv crystal structures and 3D-reconstruction of DH270.1 bound with the 92BR SOSIP.664 trimer. Superposition of backbone ribbon diagrams for DH270 lineage members: UCA1 (gray), DH270.1 (green), and DH270.6 (blue) (A) alone, (B) with the DH272 cooperating antibody (red), (C) with PGT 128 (magenta), and (D) with PGT124 (orange). Arrows indicate major differences in CDR regions, (E) Top and (F) side views of a fit of the DH270.1 Fab (green) and the BG505 SO SIP trimer (gray) into a map obtained from negative-stain EM. (G) Top and (H) side views of the BG505 trimer (PDB ID: 5ACO) (28) (gray, with V1/V2 and V3 loops highlighted in red and blue, respectively) bound with PGT124 (PDB ID: 4R2G) (27) (orange), PGT128 (PDB ID: 3TYG) ( ) (magenta), PGT135 (PDB ID: 4JM2) (22) (cyan) and DH270.1 (green), superposed. The arrows indicate the direction of the principal axis of each of the bnAb Fabs; the color of each arrow matches that of the corresponding bnAb. See also Fig. 24.

[0046] Figures 6A-B. DH270 lineage antibody binding to autologous CH848 Env components. (A) Binding of DH270 lineage antibodies (column) to 120 CH848 autologous gpl 20 Env glycoproteins (rows) grouped based on time of isolation (w: week; d: day, black and white blocks). The last three rows show the neutralization profile of the three autologous viruses that lost the PNG at position N332 (blue blocks). VI aa length of each virus is color- coded as indicated. Antibody binding is measured in ELISA and expressed as log area under the curve (LogAUC) and color-coded based on the categories shown in the histogram. The histogram shows the distribution of the measured values in each category. The black arrow indicated Env 10.17. Viruses isolated at and after week 186, which is the time of first evidence of DH270 lineage presence, are highlighted in different colors according to week of isolation. (B) Left: Binding to CH848.TF mutants with disrupted N301 and/or N332 glycan sites. Results are expressed as LogAUC. VH mutation frequency is shown in parenthesis for each antibody (see also Fig. 7 A). Middle: Binding to CH848 Env trimer expressed on the cell surface of CHO cells. Results are expressed as maximum percentage of binding and are representative of duplicate experiments. DH270 antibodies are shown in red. Palivizumab is the negative control (gray area). The curves indicate binding to the surface antigen on a 0 to 00 scale (y-axis), the highest peak between the test antibody and the negative control sets the value of 100. Right: Binding to free glycans measured on a microarray. Results are the average of background-subtracted triplicate measurements and are expressed in RU. Figures 2A-D.

[0047] Figures 7A-D. Characteristics of DH270 lineage monoclonal antibodies. (A)

Immunogenetics of DH270 lineage monoclonal antibodies. (B) Phylogenetic relationship of VHDJH rearrangements of the unmutated common ancestor (DH270.UCA) and maturation intermediates DH270.L 1 through DH270.IA4 inferred from mature antibodies DH270.1 through DH270.5. DH270.6 was not included and clusters close to DFI270.4 and DH270.5 as shown in Fig. 1. (C) Amino acid alignment of the VHDJH rearrangements of the inferred UCA and intermediate antibodies and DH270.1 through DH270.6 mature antibodies. (D) Amino acid alignment of VLJL rearrangements of the inferred UCA and intermediate antibodies and DH270.1 through DH270.6 mature antibodies. For DH270.6, all experimental data presented in this manuscript were obtained using the light chain sequence reported here. The light chain sequence of DH270.6 was subsequently revised to amino acids Q and A in positions 1 and 3 (instead of T and L). This difference did not affect neutralization and binding of DH270.6.

[0048] Figures 8A-C. DH270 lineage displays a N332-dependent V3 glycan bnAb functional profile. (A) DH270 antibody lineage neutralization of five HIV-1 pseudoviruses and respective N332A mutants. Data are expressed as IC50 μ§/ηιί. Positivity <10 μg/ml is shown in bold. (B, C) DH270.1 ability to compete gpl2() Env binding of V3 glycan bnAbs PGT1.25 and PGT128. Inhibition by cold PGT125 or PGT128 (grey line) was used as control (see Methods).

[0049] Figures 9A-D. DH475 and DH272 are strain-specific, N332-glycan dependent antibodies. (A) Phylogenetic trees of DH475 (top) and 1)1 1272 (bottom) clonal lineages. External nodes (filled circles) representing VHDJH observed sequences retrieved from cultured and sorted memory B cells (labeled) or NGS antibody sequences (unlabeled) are colored according to time point of isolation. Internal nodes (open circles) represent inferred ancestral intermediate sequences. Branch length estimates units are nucleotide substitution per site. (B) Immunogenetics of DH475 and DH272 monoclonal antibodies; (C) Binding of DH475 (top) and 1)1 1272 (bottom) monoclonal antibodies to wild-type CH848TF gp!20 Env (wild-type (wt), on the x-axis, and mutants with disrupted the 301 and/or 332 N-linked glycosylation sites. Results are expressed as LogAUC. (D) Heterologous neutralization profile of DH475 and 1)1 1272 monoclonal antibodies expressed as IC50 μg ml on a multiclade panel of 24 viruses. White square indicates IC50 > 50 μg/ml, the highest antibody concentration tested, Clades are reported on the left and virus identifiers on the right, DFI475 neutralized no heterologous viruses and DH272 neutralized one Tier 1 heterologous virus.

[0050] Figure 10. CH848 was infected by a single transmitted founder virus. 79 HIV-1 3' half single genome sequences were generated from screening timepoint plasma. Depicted is a nuci eoti de Highlighter pi ot (http : //www. hi v .1 ani . gov/content/sequence/HIGHLIGHT/ HIGHLIGHT_XYPLOT/highlighter.html). Horizontal lines represent single genome sequences and tic marks denote nucleotide changes relative to the inferred TF sequence (key at top, nucleotide position relative to FIXB2).

[0051] Figures 1 A-B. CH848 was infected by a subtype C virus. (A) PhyML was used to construct a maximum likelihood phylogenetic tree comparing the CH848 transmitted founder virus to representative sequences from subtypes Al , A2, B, C, D, Fl, F2, G, H, and K (substitution model: GTR+I+G, scale bar bottom right). The CH848 TF sequence in the subtype C virus cluster is shown in red. (B) Similarity to each subtype reference sequence is plotted on the y-axis and nucleotide position is plotted the x-axis (window size = 400 nt, significance threshold ^:= 0.95, key to right). The two bars below the x-axis indicate which reference sequence is most similar to the CH848 TF sequence ("Best Match") and whether this similarity is statistically significant relative to the second best match ("Significant").

[0052] Figure 12. Co-evolution of CH848 autologous virus and N332-dependent V3 glycan antibody lineages DH272, DH475 and DH270. Mutations relative to the CH848 TF virus in the alignment of CH848 sequences with accompanying neutralization data

(Insertion/deletions = black. Substitutions: red = negative charge; blue = positive charge; cyan = PNG sites) (43). The green line indicates the transition between DH272 DH475 sensitive and DH270 lineage sensitive virus immunotypes at day 356 (week 51 ). Viruses isolated after week 186, time of first evidence of DH270 lineage presence, are highlighted in different colors according to week of isolation.

[0053] Figures 13A-B. Mutations in CH848 Env over time. (A) Variable positions that are close to the PGT128 epitope in a trimer structure (PDB ID: 4TVP) (13) are represented by- spheres color-coded by the time post-infection when they first mutate away from the CH8 8 TF sequence. The PGT128 antibody structure (PDB ID: 5C7K) (29) was used as a surrogate for DH270, as a high resolution structure is not yet available for DH270. Env positions with either main chain, side chain or glycans within 8.5A of any PGT128 heavy atom are shown in yellow surface and brown ribbon representations. Time of appearance of mutations are color coded as indicated. (B) Same as (A) for mutating Env sites that were autologous antibody signatures of antibody sensitivity and resistance.

[0054] Figure 14. Accumulation of amino acid mutations in CH848 virus over time. This figure shows all of the readily aligned positions near the contact site of V3 glycan antibodies in Figures 13A-B, (excluding amino acids that are embedded in the VI hypervariable regions). The magenta O is a PNG site, whereas an N is an Asn that is not embedded in a glycosylation site. The logo plots represent the frequency of amino acids at each position, and the TF amino acid is left blank to highlight the differences over time.

[0055] Figure 15. CH848 virus lineage maximum likelihood phylogenetic tree rooted on the transmitted founder sequence. The phylogenetic tree shows 1,223 Env protein sequences translated from single genome sequences. Sequences sampled prior to the development of Tier 2 heterologous breadth (week 186) are shaded in grey and sequences from after week 186 are highlighted using the color scheme from Figure 12. Four viral clades with distinct DH270 lineage phenotypes are indicated with a circle, triangle, cross and "X", respectively. [0056] Figures 16A-F. Inverse-correlation between the potency of V3 giycan broadly neutralizing antibodies and VI length shown for the full panel of 207 viruses. Correlation between neutralization potency (y-axis) and VI length of the respective viruses (x-axis, n =^;: 207) of DH270 lineage bnAbs DH270.1 (A), DH270.5 (B), DH270.6 (C) and V3 giycan bnAbs 10-1074 (D), PGT121 (E) and PGT128 (F) isolated from other individuals.

Correlation p-values are non-parametric two sided, Kendall's tau. Slopes show linear regression,

|0057| Figures 17A-B. Role of VH1-2*02 intrinsic mutability in determining DH270 lineage antibody somatic hypermutation. (A) The sequence logo plot shows the frequency of VH1- 2*02 amino acid (aa) mutations from germline at each position, calculated from an alignment of 10,995 VH 1-2*02 reads obtained from 8 fflV-1 negative individuals by NGS that replicated across two independent Illumina experiments (35), The logo plot shows the frequency of mutated aa at each position. The red line indicates the threshold of mutation frequency (20%) used to define frequently mutated aa. The VH aa sequences of DH270 lineage antibodies, DH272 and VRCOl are aligned on the top. The 12 red vertical stripes indicate frequently mutated aa that were also frequently mutated (>25¾ of the VH sequences of isolated antibodies) in the DH270 lineage. (B) VH aa encoded by VHl-2 sequences from genomic DNA aligned to DH270 lineage antibodies aa sequences (see "Sequencing of germline variable region from genomic DNA" in Methods).

[0058] Figures 18A-B. Fiffect of the G57R mutation on DH270.IA4 and DH270.UCA binding to Env 10.17 gp!20. (A) Binding to Env 10.17 gpl20 by wild-type DH270.LA4 (black) and DH270.IA4 variants in which each mutated aa was reverted to germline (D3 I G, blue; I34M, orange; T55S, green, R57G, red). Mean and standard deviation from duplicate observations are indicated for each datapoint and curve fitting (non-linear, 4-parameters) is shown for each dataset. Binding is quantified as background subtracted OD450 values. (B) Binding to Env 10.17 by wild-type DH270.UCA (black) and the DH270.UCA with the G57R mutation (red).

[0059] Figure 19. Virus signature analysis. Logo plots represent the frequency of amino acids mutations in CFI848 virus quasispecies from transmitted founder at indicated positions over time. Red indicates a negatively charged amino acid, blue positive, black neutral; the light blue O is a PNG site. The signatures outlined in detail in Figure 36 are summarized in the bottom right column where a red amino acid is associated with resistance to the antibody on the right, a blue amino acid is associated with sensitivity. [0060] Figures 20A-F. Autologous Env VI length associations with DH270 lineage neutralization and gpl20 binding. Eighty-two virus Envs - the subset from Figures 34-35 that were assayed for both neutralization (A-C) and binding (D-F) to DH270. 1 , DH270.4 and DH270.5 - were evaluated. The 3 Envs that had lost the PNG site at N332 were not included, as they were negative for all antibodies tested independently of VI length. Only points from positive results are plotted: IC50 <50 ^ig/mi for neutralization in panels A-C, and AUC >\ for binding in panels D-F. N is the number of positive sample.

[0061] Figures 21 A-C. Sequence and structural comparison of DH270.UCA1 and

DH270.UCA3. Sequence alignments of UCA3 and UCAl . (A) Heavy chains and (B) light chains, whose structures were obtained in this study, are aligned with UCA4, the germline antibody for the DH270 lineage (DH270.UCA). The UCA3 and UCA4 light chains are identical. Asterisks indicate positions in which the amino acids are the same. Colon period "." and blanks " " correspond to strictly conserved, conserved and major differences, respectively, (C) Superposition of UCA3 (cyan) and UCAl (gray). Structural differences in CDR regions are indicated with an arrow.

[0062] Figure 22. Accumulation of mutation in DH270 lineage antibodies. Mutations are highlighted as spheres on the Fv region of each antibody, where the CDR regions, labeled on the backbone of the UCA, face outward. The G57R mutation is shown in red, the other mutations incurred between the UCA and IA4 are shown in orange. Mutations between intermediates are colored as follows: between IA2 and IA4, yellow; between IA1 and IA2, green; between I A3 and IA4, magenta. Mutations between the late intermediates and DH270.1, [^')! 1270.2, DH270.3, DH270.4, and Pi 1270.5 are in brown, light puipie, dark purple, blue, and dark blue, respectively,

[0063] Figures 23A-B. Negative stain EM of DH270 Fab in complex with the 92BR

SOSIP.664 trimer, (A) 2D class-averages of the complex, Fabs are indicated with a red arrow. (B) Fourier shell correlation curve for the complex along with the resolution determined using FSC ^;=: 0.5,

[0064] Figure 24. DH270.1 and other N332 bnAbs bound to the 92BR SOSIP.664 trimer. Top and side views of the BG505 trimer (PDB ID: 5 AGO) (28) (gray, with V1/V2 and V3 loops highlighted in red and blue, respectively) bound with DH270.1 (green), PGT135 (PDB ID: 4JM2) (22) (cyan), PGT124 (PDB ID: 4R2G) (27) (orange) and PGT128 (PDB ID:

3TYG) (17) (magenta) illustrate the different positions of the several Fabs on gpl40. The arrows indicate the direction of the principal axis of each of the bnAb Fabs; the color of each arrow matches that of the corresponding bnAb. [0065] Figures 25A-B. DH270.1 binding kinetics to 92BR SOSIP.664 trimers with mutated PNG sites. (A) Giycans forming a "funnel" are shown on the surface of the trimer. VI -V2 and V3 loops are colored red and blue, respectively. (B) Association and dissociation curves, using biolayer interferometry, against different 92BR SOSIP.664 glycan mutants.

[0066] Figures 26A-C. DH270.1 binding kinetics to 92BR SOSIP.664 trimer with additional mutations. (A) Sequence Logo of the V3 region of CH848 autologous viruses are shown. (B) Binding kinetics, using biolayer interferometry, against different 92BR SOSIP.664 V3 loop region mutants. (C) 1)1 1270. 1 heavy chain mutants and 92BR SOSIP.664. Biolayer interferometry association and dissociation curves for the indicated Fab mutants for binding to 92BR SOSIP.664 (600nM curves are shown) Not shown are curves for DH270.1 heavy chain mutants K32A, R72A, D73A, S25D, S54D, S60D and double mutant S75/77A for which there was little or no reduction in affinity,

[0067] Figures 27A-B. Man9-V3 giycopeptide binding of DH270 lineage antibodies. DH270 lineage tree (A, top left) is shown with VH mutations of intermediates and mature antibodies. DH270.6 niAb, which clusters close to DH270.4 and DH270.5, is not shown in the phylogenetic tree. Binding of Man9-V3 giycopeptide and its aglycone form to DH270 lineage antibodies was measured by BLI assay using either biotinylated Man9-V3 (A) or biotinylated aglycone V3 (B) as described in Methods, DFI270 lineage antibodies were each used at concentrations of 5, 10, 25, 50, 100, 150 ug/mL. Insets in (A) for UCA (150 μg/mL), IA4 (100, 50, 25 uu, ml .), IA3 and IA2 (100, 50, 25, 10 ^ig/mL) show rescaled binding curves following subtraction of non-specific signal on a control antibody (Paiivizumah). Rate (ka, kd) and dissociation constants (Kd) were measured for intermediate IA1 and mature mAbs with glycan-dependent binding to Man9-V3. Kinetics analyses were performed by global curve fitting using bivalent avidity model and as described in methods ("Affinity

measurements" section ). Inset in (B) show overlay of binding of each mAbs to Man9-V3 (blue) and aglycone V3 (red) at the highest concentration used in each of the dose titrations, [0068] Figure 28. Example of an immunization regimen derived from studies of virus-bnAb coevolution in CH848. An immunization strategy composed of the following steps: first, prime with an immunogen that binds the UCA and the boost with immunogens with the following characteristics: i. engagement of DH270,IA4-iike antibodies and selection for the G57R mutation, ii. Selection of antibodies that favor recognition of trim eric Env and expand the variation in the autologous signature residue to potentially expand recognition of diversity in population; iii. Exposing maturing antibodies to viruses with longer loops, even though these viruses are not bound or neutralized as well as viruses with shorter VI loops, as this is the main constrain on antibody heterologous population neutralization breadth ,

[0069] Figure 29. N332-dependent CH848 plasma neutralization. Fold difference in CH848 plasma neutralization IC50 of selected wild-type and N332 mutant FflV-1 strains

[0070] Figures 30A-C. NGS longitudinal sampling of VHDJH rearrangements assigned to the

DH270, DH272 and DH475 lineages from memory B cell mRNA.

[0071] Figure 31. CH848 plasma neutralization breadth over time,

[0072] Figure 32. Data collection and refinement statistics.

[0073] Figure 33. DH270 lineage heterologous neutralization (207-virus panel).

[0074] Figures 34-35. Autologous binding and neutralization of DH270 lineage, DH272 and

DH475 and heterologous neutralization on 24 virus panel.

[0075] Figure 36. Virus signatures.

[0076] Figures 37A-D. Figures 37A-37D show non-limiting embodiments of selection of immunogens to induce V3 antibodies. The figures show binding of gpl 20 envelopes listed (and/or neutralization) in the figure to various antibodies from the V3 glycan antibody lineage DH270. Figure 37A: Prime with Man9 V3 glycopeptide or aglycone. Boost (i) - expected to activate IA4, select for rare mutation; Boost (ii) ~ select for antibodies that favor the trimer with, expand the variation in the autologous signature residue to potentially expand recognition of diversity in the population. Boost (ii) in Figure 37A is optional. Boost (iii) - expected to expose the virus to longer loops, even though these viruses don't bind or neutralize as well as viruses with shooter loops, as this is the main constrain on heterologous population breadth and that is what we need. One embodiment of a V3 peptide is SEQ ID NO: 1. Figure 37D shows one non-limiting embodiment of a selection of immunogens.

These immunogens in any suitable form are expected to be used as boost(s) in the induction of V3 glycan antibodies such as but not limited to antibodies with the specificity of DH270 lineage antibodies. For priming: Man9 V3 glycopeptide or aglycone engages UCA and allows G57R to occur (i.e. UCA to IA4). Boost with CH848.d949.10.17 (VI loop length 1 7) selects IA4 with G57R. (1) CH848.d794.05.41 (VI loop length = 17) engages IA3 and IA2-Hke antibodies and further increase chances to induce DH270.1 -like antibodies. The matching virus of this envelope is still neutralized by IA3 and L42. (optional step). (2) CH848.d358.80.06 (VI loop length - 24) engages DH270.1-like antibodies and bring them to DH270.4, .5 and .6-like gradually increasing exposure to longer VI loops. The matching virus is neutralized by DH270.1 and more mature bnAbs antibodies. At this point, we may or may not already have induced bnAbs, according to the importance of exposing antibodies to longer VI loops. (3) CH848.d526.25.09 (VI loop length = 27) exposes bnAbs DH270.4 - DH27Q.6 to longer VI loop. Binding to DH270.3 is disfavored . (4) CH848.d0526.25.02 (VI loop length = 34) further exposes to even longer VI loop. There is a cost in

neutralization IC50, yet if increasing VI length is correlated with breadth, then it should bring breadth.

[0077] Figures 38A-E. Figure 38A shows synthesis of Man9 derivatized V3 glycopeptide. Chemical synthesis of oligomannose (Mang) derivatized V3 glycopeptide. (A) Chemical structure of MamGlcNA^-NEb. (B) Synthesis of Man9-V3-biotin Glycopeptide 1 - reagents and conditions: (a) Man9GicNAc2-NH2 (2), PyAOP, DIEA, DMSO; (b) Cocktail R = 90:5:3 :2 TFA/thioanisole/ethanedithiol/anisole, 32% (2 steps); (c) Man9GlcNAc₂-NH₂ (2), PyAOP, DIEA, DMSO; (d) Cocktail R - 90:5:3 :2 TFA/thioanisole/ethanedithiol/anisoie, 35% (2 steps); (e) 6 M Gnd-HCl, 200 itiM Na₂FiP04, 200 mM MPAA, 20 mM TCEP-HC1, pH 7.2; (e) 0.1 M Gnd-HCl, pH 7, 40% (2 steps). (C) Chemical structure of Aglycone V3- biotin. PyAOP ^:=: (7-Azabenzotriazol-l -yloxy)tripyrrolidinophosphonium

hexafluorophosphate; DIEA = Ν,Ν-Diisopropyiethyiamine; DMSO = dimethyl sulfoxide; TFA = trifluoroacetic acid; Gnd-HCl ^:=: guanidine hydrochloride; MPAA = 4- Mercaptophenylacetic Acid; TCEP-HCi = tris(2-carboxy ethyl )phosphine hydrochloride. The aglycone V3 peptide has SEQ ID NO: 1.

[0078] Figure 38B shows Synthetic lipid based V3 peptides for multivalent lipid nanoparticle constructs. Schematic of lipid nanoparticles with mul timers of synthetic aglycone V3 (2C) and Man9V3 glycopeptide (D). In Figure 38C and 38D both Aglycone V3 and Man9V3 peptides were synthesized with a cholesterol moiety attached via PEG linker as outlined in Figure 38B, The length of PEG linker is variable and can be short with [PEG] 3 or longer with [PEG]9 or more units. In addition to the cholesterol unit linked to the V3 peptides, the lipid composition of the lipid nanoparticle constructs include the following phospholipid combinations- POPC:POPE:DMPA:cholesterol-V3 /POPC:POPG:cholesterol-V3

/DMPCDOPG: cholesterol- V3 / POPC:sphingomyelin:cholesterol-V3, each with varying %molar of cholesterol-V3 peptide (5-28molar%). The V3 peptide to lipid ratio will be used to provide 50-200 mer V3 peptide units per lOOnm lipid nanoparticle. The multimeric V3 peptide lipid nanoparticles will be produced by methods previously described (Alam et al., 2007, 2009; Dennison et al., 2009, 2011. Figure 38E shows synthetic V3 peptides for multivalent lipid nanoparticle constructs with T peptide. The schematics shown in 2 and 3 both include the Th peptide GTH1 of the sequence shown above and will be covaiently attached to the V3 aglycone or Man9 V3 glycopeptide (as shown in 2 and 3) via a [PEG]n linker of varying units. Other Th peptide sequence can be substituted and synthesized as in constructs 2 and 3. The amphipathic GTHl sequence is also utilized for anchoring to lipid nanoparticles (Alam et al ., 2007). The lipid compositions, and peptide:lipid molar ratios used will be as described in Figures 38C and 38D.

[0079] Figures 39A-B. Figure 39A shows amino acids sequences of CH848.0949.10.17 in various forms. Various other forms can readily be obtained from the gp l60 amino acid sequence. A ski lled artisan appreciates that recombinantly produced envelope of any form do not include the signal peptide. The endogenous signal peptide of CH0848.3.D0949.10.17 gpl60 is underlined in the figure. A heterologous signal peptide of CH0848.3.D0949. 10.17 chim.6R.DS.SOSIP.664 is underlined in the figure. Figure 39B shows one embodiment of nucleic acid sequences of the designs in Figure 39A.

[0080] Figures 40A-C. Figure 40 A. shows amino acid sequences of various forms of CH848 envelopes CH848.0836.10.31 ; CH848.0358.80.06; CH848.1432.5.41 ; CH848.0526.25.02. A skilled arti san appreciates that recombinantly produced envelope of any form do not include the signal peptide. Figure 40B shows one embodiment of nucleic acid sequences of the designs in Figure 40A. Figure 40B shows one embodiment of codon optimized nucleic acid sequences of the designs in Figure 40A. Figure 40C shows the amino acids sequence as gpl60 of CH848 T/F envelope. Using the description of the various envelopes, including but not limited to SOSIP designs, the CH848 T/F envelope can also be designed in any suitable form.

[0081] Figures 41 A-C. Figure 41 A shows amino acid sequences of various forms of CH0848 envelopes. A heterologous signal peptide of CH0848.3.D0949.10. 17

chim.6R.DS. SOSIP.664 V4.1 is underlined in the figure. A skilled artisan appreciates that recombinantly produced envelope of any form do not include the signal peptide. Figure 4 IB shows one embodiment of nucleic acid sequences of the designs in Figure 41A. Figure 41C shows annotated amino acid sequence of a chimeric trimer design of

CH848.3.D0949.10.17CHIM.6R. SOSIP.664V4. L

[0082] Figures 42 and 43 show the contacts, emphasizes position D325 , and that envelope CH848 1305.10. 13 retains some binding and neutralization sensitivity. CH8481305.10.13 has a proline after the N, GDIR -> GN PR. The proline at that position is really rare. Thus envelope O 10848. . d 1 65 1 . 1 0.07. which has OMR, and residual binding to DH270.4 and Di 1270.6 is a better vaccine choice. [0083] Figures 44A-44D show amino acid and nucleic acid sequences of envelopes: >CH848 703010848.3. d0949.10.17 signature opt b gpl60 (Fig. 44A), >CH848

703010848.3 , d0949.10, 17_signature_opt_filled_rare_hol es_a gp 160 (Figure 44B),

CH0848.3.dl651.10.07 (Figure 44C), CH848

703010848.3.d0949.10.17_signature_opt_b_T250.4_Vl V^"2 (Fig, 44D). Signal peptide, furin site and delta N deletion are indicated in Fig. 44C.

[0084] Figure 45 shows amino acid sequences listed in Table 3 of Example 3 A ,

[0085] Figure 46 shows amino acid sequences of engineered VI loop variants of CH848

3.d0949.10, 17 envelope (Table 3 lines 23-25),

[0086] Figures 47A-47C shows SORTASE-C designs and sequences.

[0087] Figures 48 A-48B show screening of various envelope constructs for binding to DH270UCA4 by SPR (SPR—S200). Figure 48A shows 1)1 1270 UCA (unmutated common ancestor) binding to CH848 SOSIP gpl40 trimers. CH848.3.D0949.10.17 SOSIP trimers (lOOmg/mL) were injected over DH270JUCA captured on an anti-human Ig-Fc immobilized mAb sensor surface and binding monitored by SPR analysis on BIAcore S200 (GE

Healthcare). 1)1 1270 I .^'{^'A bound to SOSIP gp!4Q trimers but not to gp! 20 of CH848 Env, Among the CH848 trimers, more stable binding was observed with N301 A mutation, indicating that the removal of the glycan at N301 facilitate the formation of more stable complex with 1)1 1270 t^'C.A. Figure 48B shows screening of various envelopes for binding to PGT121tkUCA v:2 and DH270UCA4 Protein Panel Screening by SPR (SPR— -S200). Only J, K and O samples show binding to DH270UCA4. Figure 48C lists the names of different envelopes tested in Figure 48B. Figure 48B listing of tested envelopes is as follows:

[0088] B.JRFL gpl20core_mini_V3_v2/Kif/293F EndoH treated/Denatured 01.31 .2017 A

[0089] B.JRFL gpl20core mini V3_v2/Kif/293F EndoH treated/Native 01.31.2017 B

[0090] CH0848.3.D0949.10.17 gpl40c/5uM-Kif/293F Lot: 170131D C

[0091] CH0848.3.D0836.10.31 gpl40c/293F Lot: 17013 IB D

[0092] CH0848.3.D0949.10. 17 gpl40c/25uM-Kif/293F Lot: 170131 A E

[0093] CH0848.3.D0949.10.17 gpl40c/293F Lot: 170131C F

[0094] BG5015 MUT1 IB Dl I gpl20 _avi/293F/Mon Lot: 170130B G

[0095] CON-S gp l 40 CFI_avi V1_4Q/293F Lot: 160809C H

[0096] JRFL mini V3 gpl20 Core GNTI-/- Lot: 539HC I

[0097] CH848.3.D0949.10.17chim.6R.DS.SOSIP.664.avi_N301A/293F Lot: 20.) A.) J

[0098] CH848.3.D0949.10.17 CHIM.6R.SOSIP.664V4.1/293F Lot: 225ESD K

[0099] CH848.31⁾0949.10.17 GT1 Dl lgpl 20_avi/293F/MON Lot: 170130C L 0100] BG505_MUT1 IB Dl 1 gpl20_avi/ if/293F/Mon Lot: 170130A M

0101] B.JRFI, gpl20 Core__mini-V3__v2/Kit7293F/Mon Lot: 170130D N

0102] CH848.3.D0949.10.17chim.6R.DS.SOSIP.664/293F Lot: 226ESD O

0103] CH0848.3.D0358.80.06CHIM.6R.SOSIP.664v4.1/293F Lot: 558HC P

0104] B.JRFLgpl 40CF_aviVl 3Q/293F Lot: 160908B Q

DETAILED DESCRIPTION

[0105] The third variable region, V3, of the envelope glycoprotein, gpl20 of HIV- 1 is a target for virus broad neutralizing antibodies. Several V3 glycan dependent broad neutralizing antibodies (bnAbs) have been isolated that neutralize diverse strains of difficult to neutralize viruses. A questions remains as to what form of Env could bind and initiate V3- glycan bnAb lineages. Soluble Env gpl20 or cell surface Env trimers do not bind V3 -glycan bnAb UCAs (20) (See Example 1 Bonsignori, M et al. submitted). In some aspects the invention provides that the Man9-V3glycopeptide (Example 2) as well as its aglycone (Example 2) form binds the UCA of the DH270V3 -glycan bnAb lineage. Moreover, Mam- V3/aglycone binds to the UCA of gpl4()-induced V3 -glycan neutralizing mAb, DH501. With affinity maturation in both the DH270 bnAb and the DH501 lineages, binding to the aglycone- V3 diminished and binding to Man<>-V3 was dramatically enhanced. These observations raise the hypothesis that initiating immunogens for V3 -glycan lineages may be denatured or Env fragments (Example 1 Bonsignori, M, et al. submitted).

[0106] The invention provides various methods to choose a subset of viral variants, including but not limited to envelopes, to investigate the role of antigenic diversity in serial samples. In other aspects, the invention provides compositions comprising viral variants, for example but not limited to envelopes, selected based on various criteria as described herein to be used as immunogens. In some embodiments, the immunogens are selected based on the envelope and/or peptide binding to the UCA, and/or intermediate antibodies. In some embodiments, the immunogens are selected based on UCA and/or intermediate antibodies neutralizing properties against viruses. In some embodiments the immunogens are selected based on their chronological appearance and/or sequence diversity during infection.

[0107] In other aspects, the invention provides immunization strategies using the selections of immunogens to induce cross-reactive neutralizing antibodies,

[0108] As Example 2 shows that a synthetic homogeneous Man¾-V3 glycopeptide mimics a HIV-1 Env V3 -glycan bnAb epitope, Man9~V3 recognition by V3 -glycan memory B cell and UCA BCR suggest that a minimal V3 -glycan epitope construct may be a candidate for the induction of V3-glycan bnAb lineages. In HIV-1 infection, the DH270 V3-glycan bnAb lineage developed over ~4 years (Bonsignori, M et al. submitted), and V3-gly can-targeted antibodies took 4 years to develop in macaques repetitively immunized with Env gpl40 (Saunders, K et ai. submitted). Thus, while whole Env monomers or trimers do not bind to V3-glycan bnAb UCAs, the V3-glycopeptide does bind UCAs, suggesting that minimal Env epitopes may accelerate induction of V3-glycan bnAb B cell lineages.

[0109] Described herein are both the design and selections of immunogens to elicit neutralizing antibodies directed toward the V3 glycan epitope defined by V3 binding antibodies. Minimal V3 region glycopeptides bearing two glycans of appropriate structure can mimic the antigenic nature of this epitope, and can provide an effective platform for immunogen development. This concept— based on the "two glycans and a strand" paradigm of recognition suggested by x-ray analysis. sup.6— has been successfully applied to the V1V2 region anti -glycan BnAb site. Given the likely rarity of naive B cells relevant to BnAb ontogeny in the immune repertoire, preferred immunogens include those that exclude potentially interfering immunodominant epitopes. These immunogens can be evaluated not only based on their affinities for mature BnAbs, but also their germline precursors.

[0110] In certain embodiment, the invention provides a composition comprising any one of the inventive peptides, wherein the composition comprises purified homogenously

glycosylated peptides. In certain embodiments, about 70%, 75%, 80%, 85%, 90%>, 95%, 99%, or 99,9% of the peptides in the composition are homogenously glycosylated peptides. In certain embodiments, 70%, 75%, 80%, 85%, 90%, 95%, 99%, or 99.9% of the peptides in the composition are homogenously glycosylated peptides. In certain embodiments, 70%-75%, 75.1 %-80%, 80.1 %-85%, 85.1%-90%, 90.1%-95%, 95.1%-99%, 96%-99%, 97%-99%, 98%- 99%o or 99.9% of the peptides in the composition are homogenously glycosylated peptides. In certain embodiment, the glycosylation pattern is homogenous on all V3 peptides in the composition. In certain embodiment, the glycosylation pattern is substantially identical on ail V3 peptides in the composition,

[0111] Various methods of determining the glycosylation pattern on a peptide are known in the art. In certain embodiments, glycosylation pattern on the peptides and % homogeneity can be determined by Liquid chromatography—mass spectrometry (LC-MS, or alternatively HPLC-MS).

[0112] As indicated in the Examples that follow, V3 glycopeptides can be synthesized with well-defined glycans at N332 and N301 using clade B and clade C sequences (derived from Envs with known antigenicity toward V3 anti -glycan BnAbs). Variations of the peptide framework include full length vs. truncated V3 loops, as well as linear vs. constrained cyclic forms (via disulfide bond formation). Antigenicity testing provides the data needed to determine the peptide design motif that is optimal for binding to HIV- 1 Env anti-glycan BnAbs. Using the best peptide "scaffold", derivatives can be synthesized bearing different glycans at N332 and N301 and the determination made as to the optimal carbohydrate design for anti-glycan BnAb binding. The constructs that exhibit the highest affinity for V3 -directed anti-glycan BnAbs and their UCAs can be synthesized on larger scale and subjected to trials e.g., in non-human primates— immunogeni city can be evaluated for constructs both with and without conjugation to carrier protein.

[0113] The present invention thus relates, at least in part, to immunogens that focus the immune response to the V3 glycan epitope on gpl20 that lead to BnAbs and away from epitopes that lead to non-neutralizing antibodies. Central to the present design strategy is making the immunogen as minimal in size as possible so as not to introduce diverting, non- neutralizing epitopes. Non-limiting embodiments of immunogens are described in the Examples below,

[0114] The immunogens can be formulated with appropriate carriers using standard techniques to yield compositions suitable for administration. The compositions can include an adjuvant, such as, for example, alum, poly IC, poly IC/LC, MF-59 or other squalene-based adjuvant, ASOIB or other liposomal based adjuvant suitable for protein immunization.

Suitable vaccine strategies include, e.g., those described, for in the Examples that follow.

[0115] Nucleic acid sequences (e.g., DNA sequences) encoding the immunogens can also be administered to a subject (e.g., a human) under conditions such that the immunogen is expressed in vivo and BNAbs are produced. The DNA can be present as naked DNA with a potent promoter such as the CMV promoter as used in the pCMVr plasmid (Churchyard et al, PLoS One 6:e21225 (2011)) or as an insert in a vector, such as a r Adenoviral (Barouch, et al. Nature Med. 16: 319-23 (2010), recombinant mycobacterial (i.e., BCG or M smegmatis) (Yu et al. Clinical Vaccine Immunol. 14: 886-093 (2007), ibid 13: 1204-1 1 (2006), or

recombinant vaccinia type of vector (Santra S. Nature Med. 16: 324-8 (2010)).

[0116] Immunogens of the invention, and nucleic acids (e.g., DNAs) encoding same, are suitable for use in generating an immune response (e.g., BNAbs) in a patient (e.g., a human patient) to HIV-1. The V3 N301, N332 peptide glycan can optimally be administered as a peptide-glycan formulated in a squalene based adjuvant such as MF59, or GLA-SE (Aiving et al, Current Opinion in Immunology 24:310 (2012)). The mode of administration of the immunogen, or encoding sequence, can vary with the particular immunogen, the patient and the effect sought, similarly, the dose administered. Typically, the administration route is intramuscular or subcutaneous injection (intravenous and intraperitoneal can also be used). Additionally, the formulations can be administered via the intranasal route, or iiitrarectally or vaginally as a suppository-like vehicle. Optimum dosing regimens can be readily determined by one skilled in the art. The immunogens (and nucleic acids encoding same) are preferred for use prophylacticaliy, however, their administration to infected individuals may reduce viral load,

[0117] The present invention includes the specific protein immunogens disclosed herein and nucleic acids comprising nucleotide sequences encoding same. The proteins can be expressed, for example, in 293 T cells, 293F cells or CHO ceils (Liao et ai, Virology 353 :268- 82 (2006))

[0118] Peptides

[0119] The polypeptides of the present invention may be fused to or chemically linked with an appropriate carrier molecule, such as tetanus toxin, MLv gp70, cholera toxin, keyhole limpet haemocyanin or gpl20. Alternatively, the polypeptides of the present invention may^¬ be inserted by genetic engineering techniques into permissi ble exposed loops of antigenic proteins.

[0120] Versions of the constructs that are conjugated to carrier protein will be produced for the purposes of comparison. Carrier proteins used in currently licensed vaccines include tetanus toxoid (TT), diphtheria toxoid (DT), CRM. sub.197 (cross-reactive material of diphtheria toxin. sub.197), N. meningitidis outer membrane protein (OMP), and H. influenzae protein D.. up.64 For the initial studies, CRM. sub.197, a non-toxic mutant (G52.fwdarw.D) of diphtheria toxin, will be selected which, unlike TT and DT, does not require chemical detoxification with formaldehyde. Thus, it is a well-defined, homogeneous 63 kD protein with a complete set of free, surface-exposed lysine chains (39 total), devoid of cross-linking, which are available for conjugation with potential haptens.. sup.65 Keyhole limpet hemocyanin (KLH) would be a potential alternative.

[0121] Alternatively the polypeptides of the present invention may be linked to amino acids derived from a T-helper epitope to enhance their immunogenicity.

[0122] A T-helper epitope is a peptide capable of activating a T helper cell. The T-helper epitope may be a human immunodeficiency virus (HIV) T helper epitope e.g. from the C4 domain of HIV gpl20. According to one embodiment, the T helper epitope comprises about 16 consecutive residues from the C4 domain (about residues 421 to 436). According to another embodiment, the T-helper sequence is a variation of the above. [0123] Contemplated T helper epitopes from the C4 domain are described in U.S. Pat. Appl. No. 20030147888, incorporated herein by reference. Other T helper determinants from HIV or from non-HIV proteins can also be used. For example, a further T helper epitope suitable for use in the invention is from HIV gag (e.g., residues 262-278). One such sequence is designated GTH1 , Variants of this sequence can also be used.

[0124] Another contemplated T helper epitope is derived from murine HSP60 458-474.

[0125] In some embodiments, a carbohydrate such as the outer membrane protein of pneumococcus, or another carbohydrate or protein with immunogenic, T helper activity can be used.

[0126] The T-helper epitope amino acids may be linked to the V3 portion of the peptides of the present invention using any method known in the art so long as it does not decrease the immunogenic and antigenic properties of the peptide.

[0127] The amino acids of the V3 domain of gp^'120 are preferably linked C terminal to the amino acids of the T-helper epitope.

[0128] According to one embodiment, the V3 portion of the polypeptide is linked to the T helper epitope via a covalent bond (e.g. a peptide bond). According to another embodiment, the V3 portion of the polypeptide is linked to the T helper epitope via a non-covalent linker. The linkage may be direct or via bonding to an intervening linker element, such as a linker peptide or other chemical moiety, such as an organic polymer.

[0129] Any suitable method for conjugating the V3 portion with the T helper epitope portion are known in the art.

[0130] Natural aromatic amino acids, Trp, Tyr and Phe, may be substituted for synthetic non- natural acid such as Phenylglycine, TIC, naphthylelanine (Nol), ring-methylated derivatives of Phe, halogenated derivatives of Phe or o-methyl-Tyr.

[0131] In addition to the above, the polypeptides of the present invention may also include one or more modified amino acids or one or more non-amino acid moieties (e.g. lipids, complex carbohydrates etc). In some embodiments, these non-amino acid moieties are used to multimerize the peptides of the invention.

[0132] Amino acids incorporated in the peptides of the invention could include the 20 naturally occurring amino acids, D- and L-amino acids (stereoisomers); those amino acids often modified post-translationally in vivo, including, for example, hydroxy proline, phosphoserine and phosphothreonine; and other unusual amino acids including, but not limited to, 2-aminoadipic acid, hydroxylysine, isodesmosine, nor-valine, nor-leucine and ornithine. [0133] Sequences/Clones

[0134] Described herein are nucleic and amino acids sequences of HIV- 1 envelopes. The sequences for use as immunogens are in any suitable form. In certain embodiments, the described HTV-1 envelope sequences are gpl60s. In certain embodiments, the described HIV-1 envelope sequences are gpl20s. Other sequences, for example but not limited to stable SOSIP trimer designs, gpl45s, gpl40s, both cleaved and uncleaved, gpl40 Envs with the deletion of the cleavage (C) site, fusion (F) and immunodominant (I) region in gp41 ~ named as gpMOACFI (gpMOCFI), gpl40 Envs with the deletion of only the cleavage (C) site and fusion (F) domain -- named as gpMOACF (gpMOCF), gpl40 Envs with the deletion of only the cleavage (C)— named gpl40AC (gp l 40C) (See e.g. Liao et ai. Virology 2006, 353, 268-282), gplSOs, gp41s, which are readily derived from the nucleic acid and amino acid gpl oO sequences. In certain embodiments the nucleic acid sequences are codon optimized for optimal expression in a host cell, for example a mammalian cell, a rBCG cell or any other suitable expression system.

[0135] An HIV-1 envelope has various structurally defined fragments/forms: gpl60; gp^'140— -including cleaved gpl 40 and uncleaved gpl40 (gpMQC), gpMQCF, or gpMOCFI; gpl20 and gp41. A skilled artisan appreciates that these fragments/forms are defined not necessarily by their crystal structure, but by their design and bounds within the full length of the gpl60 envelope. While the specific consecutive amino acid sequences of envelopes from different strains are different, the bounds and design of these forms are well known and characterized in the art.

[0136] For example, it is well known in the art that during its transport to the cell surface, the gpl60 polypeptide is processed and proteolytically cleaved to gpl20 and gp41 proteins. Cleavages of gpl60 to gpl20 and gp 1 occurs at a conserved cleavage site "REKR." See Chakrabarti et al. Journal of Virology vol. 76, pp. 5357-5368 (2002) see for example Figure 1, and Second paragraph in the Introduction on p. 5357; Binley et al. Journal of Virology vol. 76, pp. 2606-2616 (2002) for example at Abstract; Gao et al . Journal of Virology vol. 79, pp. 1154-1 163 (2005); Liao et al. Virology vol. 353(2): 268-282 (2006).

[0137] The role of the furin cleavage site was well understood both in terms of improving cleave efficiency, see Binley et al. supra, and eliminating cleavage, see Bosch and Pawlita, Virology 64 (5):2337-2344 (1990); Quo et al. Virology 174: 217-224 (1990); McCune et al. Cell 53 :55-67 (1988); Liao et al. J Virol. Apr;87(8):4185-201 (2013).

[0138] Likewise, the design ofgpl40 envelope forms is also well known in the art, along with the various specific changes which give rise to the gpl4()C (uncleaved envelope), gpl40CF and gpl40CFI forms. Envelope gpl40 forms are designed by introducing a stop codon within the gp41 sequence. See Chakrabarti et al. at Figure 1.

[0139] Envelope gpl 40C refers to a gpl40 HIV-1 envelope design with a functional deletion of the cleavage (C) site, so that the gpl40 envelope is not cleaved at the furin cleavage site. The specification describes cleaved and uncleaved forms, and various furin cleavage site modifications that prevent envelope cleavage are known in the art. In some embodiments of the gpl4()C form, two of the R residues in and near the furin cleavage site are changed to E, e.g., RRWEREKR is changed to ERVVEREKE, and is one example of an uncleaved gpl40 form. Another example is the gpl40C form which has the REKR site changed to SEKS. See supra for references.

[0140] Envelope gpl40CF refers to a gpl40 HIV- 1 envelope design with a deletion of the cleavage (C) site and fusion (F) region. Envelope gpl40CFI refers to a gpl40 HIV-1 envelope design with a deletion of the cleavage (C) site, fusion (F) and immunodominant (I) region in gp41. See Chakrabarti et al. Journal of Virology vol. 76, pp. 5357-5368 (2002) see for example Figure 1, and Second paragraph in the Introduction on p. 5357; Binley et al. Journal of Virology vol . 76, pp. 2606-2616 (2002) for example at Abstract; Gao et al. Journal of Virology vol. 79, pp. 1154-1163 (2005); Liao et al. Virology vol. 353(2): 268-282 (2006).

[0141] In certain embodiments, the envelope design in accordance with the present invention involves deletion of residues (e.g., 5-1 1, 5, 6, 7, 8, 9, 10, or 1 amino acids) at the N- terminus. For delta N-terminal design, amino acid residues ranging from 4 residues or even fewer to 14 residues or even more are deleted. These residues are between the maturation (signal peptide, usually ending with CXX, X can be any amino acid) and "VPVXXXX.

In one embodiments, CH0848.3.D0949.10.17 Delta 11 gpl20 is shown as an example in Figure 3 A. In certain embodiments, the invention relates generally to an immunogen, gpl60, gpl 20 or gpl 40, without an N-terminal Herpes Simplex gD tag substituted for amino acids of the N-terminus of gpI20, with an HIV leader sequence (or other leader sequence), and without the original about 4 to about 25, for example 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 amino acids of the N-terminus of the envelope (e.g. gpl20). See WO2013/006688, e.g. at pages 10-12, the contents of which publication is hereby incorporated by reference in its entirety.

[0142] The general strategy of deletion of N-terminal amino acids of envelopes results in proteins, for example gpi20s, expressed in mammalian cells that are primarily monomeric, as opposed to dimeric, and, therefore, solves the production and scalability problem of commercial gpl20 Env vaccine production. In other embodiments, the amino acid deletions at the N-terminus result in increased immunogenicity of the envelopes.

[0143] In certain embodiments, the invention provides envelope sequences, amino acid sequences and the corresponding nucleic acids, and in which the V3 loop is substituted with the following V3 loop sequence TRPNNNTRKSIRIGPGQTFY ATGD IIG RQAH, This substitution of the V3 loop reduced product cleavage and improves protein yield during recombinant protein production in CHO cells.

[0144] Soluble trimers comprising CH848 envelopes are contemplated by the invention and such trimer are contemplated for use in the methods of the invention. Various ways to form soluble envelope trimers are known in the art. See e.g. US Pub. 20100041875; US Pub 201 0076298; US Pub. 201 0250220; WO2016/037154, de Taeye et al. Cell. 2015 Dec 17; 163(7): 1702-15. doi: 10.1016/j . cell .2015.11 , 056,; Kwon et al . Nat Struct Mol Biol. 2015 Jui;22(7): 522-31. doi: 10.1038/nsmb.3051. Epub 2015 Jun 22; Sharma et al. Ceil Rep. 2015 Apr 28, 11(4):539-50. doi : 10. 1016/j .celrep.2015.03.047. Epub 2015 Apr 16 all of these publications are incorporated by reference in their entirety. The invention provides new chimeric designs, for example but not limited to

CH848.3.D0949.10.17CHIM.6R.SOSIP.664V4.1 (Figure 41C).

[0145] Non-limiting examples of trimer sequence designs are shown in Figures 39A-B, 40A- C, and 41 A-C. In some embodiments, the HIV-l envelope trimer complex incorporated some aspects of the SOSIP HIV-l trimer design.

[0146] Properties of the trimer complexes of the invention can be determined by any suitable assay used to characterize trimer envelope complexes. Antigenicity of the trimers, for example binding to HIV-l antibodies, including but not limited to antibodies described in the invention, conformational state of the trimers, i.e., "open" or "closed", immunogenicity can be determined by any suitable assay. For discussion on open versus closed envelope confirmation see de Taeye et al. Cell. 2015 Dec 17; 163(7): 1702-15; Munro et a.; Science 07 Nov 2014: Vol. 346, Issue 6210, pp. 759-763, DOI: 10.1126/science.1254426; Guttman et al., Nature Communications 6, Article number: 6144 doi: 10.1038/ncomms7144.

[0147] In certain aspects, the invention provides composition and methods which use a selection of Envs, as gpl20s, gp 140s cleaved and uncleaved, gpl45s, gpl50s and gp!60s, as proteins, as monomers or trimers, as DNAs, as RNAs, or any combination thereof, administered as primes and boosts to elicit immune response. Envelopes as proteins could be co-administered with nucleic acid vectors containing Envs to amplify antibody induction. In certain embodiments, the compositions and methods include any immunogenic HIV-1 sequences to give the best coverage for T cell help and cytotoxic T cell induction. In certain embodiments, the compositions and methods include mosaic and/or consensus HIV-1 genes to give the best coverage for T cell help and cytotoxic T cell induction. In certain

embodiments, the compositions and methods include mosaic group M and/or consensus genes to give the best coverage for T cell help and cytotoxic T cell induction. In some embodiments, the mosaic genes are any suitable gene from the HIV-1 genome. In some embodiments, the mosaic genes are Env genes, Gag genes, Pol genes, Nef genes, or any combination thereof. See e.g. US Patent No. 7951377. In some embodiments the mosaic genes are bivalent mosaics. In some embodiments the mosaic genes are trivalent. In some embodiments, the mosaic genes are administered in a suitable vector with each immunization with Env gene inserts in a suitable vector and/or as a protein. In some embodiments, the mosaic genes, for example as bivalent mosaic Gag group M consensus genes, are

administered in a suitable vector, for example but not limited to HSV2, would be

administered with each immunization with Env gene inserts in a suitable vector, for example but not limited to HSV-2.

10148 f In certain aspects the invention provides compositions and methods of Env genetic immunization either alone or with Env proteins to recreate the swarms of evolved viruses that have led to bnAb induction, Nucleotide-based vaccines offer a flexible vector format to immunize against virtually any protein antigen. Currently, two types of genetic vaccination are available for testing— DNAs and mRNAs.

[0149] In certain aspects the invention contemplates using immunogenic compositions wherein immunogens are delivered as DNA. See Graham BS, Enama ME, Nason MC, Gordon IJ, Peel SA, et ai. (2013) DNA Vaccine Delivered by a Needle-Free Injection Device Improves Potency of Priming for Antibody and CD8+ T-Cell Responses after rAd5 Boost in a Randomized Clinical Trial. PLoS ONE 8(4): e59340, page 9. Various technologies for delivery of nucleic acids, as DNA and/or RNA, so as to elicit immune response, both T-cell and humoral responses, are known in the art and are under developments. In certain embodiments, DNA can be delivered as naked DNA. In certain embodiments, DNA is formulated for delivery by a gene gun. In certain embodiments, DNA is administered by electroporation, or by a needle-free injection technologies, for example but not limited to Biojector® device. In certain embodiments, the DNA is inserted in vectors. The DNA is delivered using a suitable vector for expression in mammalian ceils. In certain embodiments the nucleic acids encoding the envelopes are optimized for expression. In certain embodiments DNA is optimized, e.g. codon optimized, for expression. In certain embodiments the nucleic acids are optimized for expression in vectors and/or in mammalian cells. In non-limiting embodiments these are bacterial ly derived vectors, adenovirus based vectors, rAdenovirus (e.g. Barouch DH, et al. Nature Med. 16: 319-23, 2010), recombinant mycobacteria (e.g. rBCG or M smegmatis) (Yu, JS et al . Clinical Vaccine Immunol. 14: 886- 093,2007; ibid 13 : 1204-11,2006), and recombinant vaccinia type of vectors (Santra S.

Nature Med. 16: 324-8, 2010), for example but not limited to ALVAC, replicating (Kibler KV et al., PLoS One 6: e25674, 2011 nov 9.) and non-replicating (Perreau M et al. J.

virology 85: 9854-62, 201 1) NYVAC, modified vaccinia Ankara (MVA)), adeno-associated virus, Venezuelan equine encephalitis (VEE) replicons, Herpes Simplex Virus vectors, and other suitable vectors.

[0150] In certain aspects the invention contemplates using immunogenic compositions wherein immunogens are delivered as DNA or RNA in suitable formulations. Various technologies which contemplate using DNA or RNA, or may use complexes of nucleic acid molecules and other entities to be used in immunization. In certain embodiments, DNA or RNA is administered as nanoparticles consisting of low dose antigen-encoding DNA formulated with a block copolymer (amphophilic block copolymer 704). See Cany et al., Journal of Hepatology 201 1 vol. 54 j 115-121 ; Arnaoty et al., Chapter 17 in Yves Bigot (ed.), Mobile Genetic Elements: Protocols and Genomic Applications, Methods in Molecular Biology, vol. 859, pp293-305 (2012), Arnaoty et al. (2013) Mol Genet Genomics. 2013 Aug;288(7-8):347-63. Nanocarrier technologies called Nanotaxi® for immunogenic macromolecules (DNA, RNA, Protein) deliver}' are under development. See for example technologies developed by Incellart. In certain embodiments, the nucleic acids, for e.g.

mRNAs encoding immunogens of the invention, are delivered by a lipid nanoparticle (LNP) technology. In non-limiting embodiments, the LNPs could comprise four different lipids that could self assemble to 80-100nm size partciles.

[0151] In certain aspects the invention contemplates using immunogenic compositions wherein immunogens are delivered as recombinant proteins. Various methods for production and purification of recombinant proteins suitable for use in immunization are known in the art. In certain embodiments recombinant proteins are produced in CHO ceils.

[0152] The immunogenic envelopes can also be administered as a protein boost in

combination with a variety of nucleic acid envelope primes (e.g., HIV -1 Envs delivered as DNA expressed in viral or bacterial vectors). [0153] Dosing of proteins and nucleic acids can be readily determined by a skilled artisan. A single dose of nucleic acid can range from a few nanograms (ng) to a few micrograms (μ ) or milligram of a single immunogenic nucleic acid. Recombinant protein dose can range from a few ^ig micrograms to a few hundred micrograms, or milligrams of a single immunogenic polypeptide.

[0154] Administration: The compositions can be formulated with appropriate carriers using known techniques to yield compositions suitable for various routes of administration. In certain embodiments the compositions are delivered via intramascular (IM), via

subcutaneous, via intravenous, via nasal, via mucosal routes, or any other suitable route of immunization.

[0155] The compositions can be formulated with appropriate carriers and adjuvants using techniques to yield compositions suitable for immunization. The compositions can include an adjuvant, such as, for example but not limited to, alum, poly IC, MF-59 or other squaiene- based adjuvant, ASOIB, or other liposomal based adjuvant suitable for protein or nucleic acid immunization. In certain embodiments, the adjuvant is GSK AS01E adjuvant containing MPL and QS21 . This adjuvant has been shown by GSK to be as potent as the similar adjuvant ASOIB but to be less reactogenic using HBsAg as vaccine antigen [Leroux-Roels et al., lABS Conference, April 2013,9]. In certain embodiments, TLR agonists are used as adjuvants. In other embodiment, adjuvants which break immune tolerance are included in the immunogenic compositions.

[0156] In certain embodiments, the compositions are formulated such that the immunoges are comprises in nanoparticles. In some embodiments, these are lipid nanoparticle immunogens. In some embodiments, these are liposomes comprising immunogens. In some embodiments these are lipid nanodiscs. The immunogens could be arranged as particulate, high-density array on liposomes or other particles, for example but not limited to nanoparticles. In non- limiting embodiment, the liposome comprises cholesterol, PC, PE, PA, or any combination thereof. See Alam et al. J Immunol. 2007 Apr l ; 178(7):4424-35; Alam et al. J Virol, 2008 Jan;82(l): 115-25; Alam et al. Proc Natl Acad Sci U S A. 2009 Dec l ; 106(48):20234-9. doi: 10.1073/pnas.0908713106; Dennison et al. J Virol. 2009 Oct;83(19): 10211-23. doi:

10.1128/JVI.00571 -09; Dennison et al. PLoS One. 2011;6(l l):e27824. doi:

10.1371/journal.pone.0027824. In some embodiments, the lipid composition of lipid nanoparticle comprises cholesterol, POPC, sphingomyelin, or any combination thereof. In some embodiments, the lipids could comprise POPC, POPE, DMPA, cholesterol, or any combination thereof. In some embodiments, the ratio is POPC:POPE:DMPA:Cholesterol 45 :25 :20: 1.33. In some embodiments, the protein to lipid ratio is about 1 :3000. In some embodiments, the peptide to lipid ratio used provides 50-100 mer V3 peptide units per 100- 200nm lipid nanoparticle. In some embodiments the peptide:lipid ratio is 1 : 100. A skilled artisan can readily determine conditions and lipids to achieve different desired ratios.

[0157J In certain embodiments, the compositions and methods comprise any suitable agent or immune modulation which could modulate mechanisms of host immune tolerance and release of the induced antibodies. In non-limiting embodiments modulation includes PD-1 blockade; T regulatory cell depletion; anti-CD25 antibodies; CD40L hyperstimulation; anti-CTLA4 antibodies, soluble antigen administration, wherein the soluble antigen is designed such that the soluble agent eliminates B cells targeting dominant epitopes, or a combination thereof. In certain embodiments, an immunomodulatory agent is administered in at time and in an amount sufficient for transient modulation of the subject's immune response so as to induce an immune response which comprises broad neutralizing antibodies against HTV- 1 envelope. Non-limiting examples of such agents is any one of the agents described herein: e.g.

chloroquine (CQ), PTP1B Inhibitor - CAS 765317-72-4 - Calbiochem or MSI 1436 clodronate or any other bisphosphonate; a Foxol inhibitor, e.g. 344355 j Foxol Inhibitor, AS 1842856 - Calbiochem; Gleevac, anti-CD25 antibody, anti-CCR4 Ab, an agent which binds to a B cell receptor for a dominant HIV-1 envelope epitope, or any combination thereof. In non-limiting embodiments, the modulation includes administering an anti-CTLA4 antibody. Non-limiting examples are ipilimumab and tremelimumab. In certain

embodiments, the methods comprise administering a second immunomodulatory agent, wherein the second and first immunomodulatory agents are different.

[0158] Table J Summary of some disclosure of proteins and sequences.

0949.10,18 aa 41A

One

embodiment of

a nucleic acid

CH848 T/F Fig 40C

[0159] It is readily understood that the envelope glycoproteins referenced in various examples and figures comprise a signal/leader sequence. It is well known in the art that HIV- 1 envelope glycoprotein is a secretory protein with a signal or leader peptide sequence that is removed during processing and recombinant expression (without removal of the signal peptide, the protein is not secreted). See for example Li et al . Control of expression, glycosylation, and secretion of HIV- 1 gpl20 by homologous and heterologous signal sequences. Virology 204(l):266-78 ( 1994) ("Li et al. 1994"), at first paragraph, and Li et al. Effects of inefficient cleavage of the signal sequence of HIV- 1 gpl20 on its association with calnexin, folding, and intracellular transport. PNAS 93 :9606-9611 (1996) ("Li et al . 1996"), at 9609. Any suitable signal sequence could be used. In some embodiments the leader sequence is the endogenous leader sequence. Most of the gpl20 and gp!60 amino acid sequences include the endogenous leader sequence. In other non-limiting examples the leaders sequence is human Tissue Plasminogen Activator (TP A) sequence, human CD 5 leader sequence (e.g. MPMGSLQPLATLYLLGMLVASVLA). Most of the chimeric designs include CDS leader sequence. A skilled artisan appreciates that when used as immunogens, and for example when recombinantly produced, the amino acid sequences of these proteins do not comprise the leader peptide sequences.

[0160] The invention is described in the following non-limiting examples.

[0161] Nomenclature for trimers: chim.6R.DS.SOSIP.664 is SOSIP.I CHTM.6R.SGSIP.664 is SOSIP.II, CHIM.6R.SQSIP.664V4. I is SOSIP.III.

EXAMPLES

[0162] The following specific examples are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever. Without further elaboration, it is believed that one skilled in the art can, based on the description herein, utilize the present invention to its fullest extent.

Example 1 Staged induction of HIV- 1 glycan-dependent broadly neutralizing antibodies

[0163] Stages of V3-glycan neutralizing antibody maturation are identified that explain the long duration required for their development. [0164] Abstract

[0165] A preventive HIV-1 vaccine should induce HIV-1 specific broadly neutralizing antibodies (bnAbs). However, bnAbs generally require high levels of somatic hypermutation (SHM) to acquire breadth and current vaccine strategies have not been successful in inducing bnAbs, Since bnAbs directed against a glycosylated site adjacent to the third variable loop (V3) of the HIV-1 envelope protein require limited SHM, the V3 glycan epitope is a desirable vaccine target. By studying the cooperation among multiple V3 -glycan B-cell lineages and their co-evolution with autologous virus throughout 5 years of infection, we identify here key events in the ontogeny of a V3-giycan bnAb. Two autologous neutralizing antibody lineages selected for virus escape mutations and consequently allowed initiation and affinity maturation of a V3 -glycan bnAb lineage. The nucleotide substitution required to initiate the bnAb lineage occurred at a low probability site for activation-induced cytidine deaminase activity. Cooperation of B-cell lineages and an improbable mutation critical for bnAb activity define the necessary events leading to V3 -glycan bnAb development, explain why initiation of V3 -glycan bnAbs is rare, and suggest an immunization strategy for inducing V3- glycan bnAbs.

[0166] Introduction

[0167] A vaccine to prevent HIV-1 infection should include immunogens that can induce broadly neutralizing antibodies (bnAbs) (7, 2). Of the five major targets for bnAbs, the glycan -rich apex of the HIV-1 envelope (Env) trimer and the base of the third variable loop (V3 ) are distinguished by the potency of antibodies directed against them (3-8). Although these antibodies have less breadth than those directed against the CD4 binding site (CD4bs) or the gp41 membrane-proximal region (MPER), one current goal of vaccine development is to elicit them in combination with other bnAb specificities to achieve broad coverage of transmitted/founder (TF) viruses to prevent HIV-1 integration upon exposure (/, 2).

[0168] Mapping the co-evolution of vims and antibody lineages over time informs vaccine design by defining the succession of HIV-1 Env variants that evolve in vivo during the course of bnAb development (9-11). Antibody lineages with overlapping specificities can influence each other's affinity maturation by selecting for synergistic or antagonistic escape mutations: an example of such "cooperating" lineages is provided by two CD4bs-directed bnAbs that we characterized previously (11, 12). Thus, cooperating antibody lineages and their viral escape mutants allow identification of the specific Envs, among the diverse repertoire of mutated Envs that develop within the autologous quasi-species in the infected individual, that stimulate bnAb development and that we wish to mimic in a vaccine. [0169] Here we describe the co-evolution of an HIV-1 Env quasispecies and a memory IB- cell lineage of gpl20 V3-giycan directed bnAbs in an acutely infected individual followed over time as broadly neutralizing plasma activity developed. To follow virus evolution, we sequenced -1,200 HIV-1 env genes sampled over a 5 year period; to follow the antibody response, we identified natural heavy- and light-chain pairs of six antibodies from a bnAb lineage, designated DH270, and augmented this lineage by next generation sequencing (NGS). Structural studies defined the position of the DH270 Fab on gpl40 Env, We also found two B-cell lineages (DH272 and DH475) with neutralization patterns that likely selected for observed viral escape variants, which in turn stimulated the DH270 lineage to potent neutralization breadth. VVe found a mutation in the DH270 heavy chain that occurred early in affinity maturation at a disfavored activation-induced cytidme deaminase (AID) site and that was necessary for bnAb lineage initiation. This improbable mutation can explain the long period of antigenic stimulation needed for initial expansion of the bnAb B-cell lineage in this individual.

[0170] Results

[0171] Three N332 V3-glycan dependent antibody lineages

[0172] We studied an African male from Malawi (CH848) followed from the time of infection to 5 years post-transmission. He was infected with a clade C virus, developed plasma neutralization breadth 3.5 years post-transmission and did not receive antiretroviral therapy during this time as per country treatment guidelines. Reduced plasma neutralization of N332A Env-mutated HIV-1 pseudoviruses and plasma neutralization fingerprinting demonstrated the presence of N332-sensitive broadly neutralizing antibodies (bnAbs) (Fig. 29) (75). To identify these antibodies, we studied memory B cells from weeks 205, 232, and 234 post-infection using memory B cell cultures (14) and antigen-specific sorting (75, 16) and found three N332-sensitive lineages, designated DH270, DH272 and DH475. Their genealogy was augmented by NGS of memory B-cell cDNA from seven time points spanning week 1 1 to week 240 post-transmission.

[0173] DH270 antibodies were recovered from memory B cells at all three sampling times (weeks 205, 232, and 234) and expansion of the clone did not occur until week 186 (Fig. 1 A and Figs. 30A-C). Clonal expansion was concurrent with development of plasma

neutralization breadth (Fig. 31), and members of the DH270 lineage also displ ayed neutralization breadth (Fig. I B and Fig. 33). The most potent 1)1 1270 lineage bnAb

(DH270.6) was isolated using a fluorophore-labeied Man9-V3 glycopeptide that is a mimic of the V3-glycan bnAb epitope (16) comprising a discontinuous 30 amino acid residue peptide segment within gpl20 V3 and representative of the PGT128-bound minimal epitope described by Pejchal et al . (17). The synthetic Man9-V3 glycopeptide includes high rnannose glycan residues (Mam) each at N301 and N332 and was synthesized using a chemical process similar to that described previously (18, 19). V3 glycan bnAb PGT128 affinity for the Mang- V3 glycopeptide was similar to that of PGT128 for the BG505 SOSIP trimer and Maii9-V3 glycopeptide was therefore an effective affinity bait for isolating of V3 glycan bnAbs (16). The lineage derived from a VH1-2*02 rearrangement that produced a CDRH3 of 20 amino acid residues paired with a light chain encoded by V>J2-23 (Figs. 7A-D). Neutralization assays and competition with V3 -glycan bnAbs PGT125 and PGT128 confirmed lineage N332- dependence (Figs. 8A-C).

[0174] The DH475 mAb was recovered from memory B cells at week 232 post-transmission by antigen-specific sorting using the fluorophore-labeled Man9-V3 glycopeptide (16). The earliest DH475 lineage VHDJH rearrangements were identified with NGS at week 64 post- transmission (Fig. 9A and Figs. 30A-C). Its heavy chain came from VH3-23*01 (VH mutation frequency = 10.1%) paired with a VD4-69*02 light chain (Fig. 9B),

[0175] The DH272 mAb came from cultured memory B cells obtained at week 205 post- transmission. DH272 lineage VHDJH rearrangements were detected as early as 19 weeks post-transmission by NGS (Fig. 9A and Figs. 30A-C). The DH272 heavy chain used VHI - 2*02, as did DH270, but it paired with a V 2-30 light chain. Its CDRH3 was 17 amino acids long; VH mutation was 14.9%. DH272, an IgA isotype, had a 6-nt deletion in FRH3 (Fig. 9B).

[0176] For both DH272 and DH475 lineages, binding to CH848 TF Env gpl20 depended on the N332 potential N -l i nked glycosylation (PNG) site (Fig. 9C). 1)1 1272 binding also depended on the N301 PNG site (Fig. 9C). Neither lineage had neutralization breadth (Fig. 9D).

[0177] Evolution of the CH848 virus quasispecies

[0178] We sequenced 1,223 HIV-1 3'-half single-genomes from virus in plasma collected at 26 time points over 246 weeks. Analysis of sequences from the earliest plasma sample indicated that CH848 had been infected with a single, subtype clade C founder virus, - 17 (CI 14-19) days prior to screening (Figs. 10 and 1 1A-B). By week 51 post-infection, 91% of the sequences had acquired an identical, 10-residue deletion in variable loop 1, a region that includes the PGT128-proximal residues 133-135 and 141 (Figs. 12 and 13A-B). Further changes accrued during the ensuing four years, including additional insertions and deletions (indels) in VI , mutations in the ³²⁴GDIR³²⁷ motif within the V3 loop, deletion or shifting of N-linked glycosylation sites at positions 301 and 322, and mutations at PGT128-proximal positions in VI , V3, and C4, but none of these escape variants went to fixation during 4.5 years of follow-up (Figs. 12-15).

[0179] Simultaneously with the first detection of DH270 lineage antibodies at week 186, four autologous virus clades emerged that defined distinct immunological resistance profiles of the CH848 autologous quasi species (Fig. 12). The first clade included viruses that shifted the potential N-glycosylation (PNG) site at N332 to 334 (Fig. 12, open circles) and despite this mutation was associated with complete resistance to the DH270 lineage bnAbs, this clade was detected only transiently and at relatively low frequency (7-33% per sample), suggesting a balance where immune escape was countered by a cost in virological fitness. Conversely, viruses in the other three clades retained N332 and persisted throughout the 5 years of sampling. Viruses in the second ciade resisted DH270 lineage neutralization and comprised gpl20 Envs that were not bound by the DH270 antibodies (Fig, 12, triangles and Figs, 34- 35). The third and fourth clades defined autologous viruses whose gpl20 Env was bound by DH270 lineage antibodies but that were either only weakly neutralized by the most mature members of the DH270 lineage (Fig. 12, "X" and Figs. 34-35) or were completely

neutralization resistant (Fig. 12, "+" and Figs, 34-35), respectively. Persistence of four divergent clades in the CH848 Env, each with distinctive immunological resistance phenotypes, suggests that multiple distinctive immune escape routes were explored and selected, allowing continuing Env escape mutations to accaie in distinct frameworks and exposing the antibody to Env diversity that may have been necessary to acquire neutralization breadth.

[0180] Ontogeny of DH270 lineage and acquisition of neutralization breadth

[0181] As with other V3-glycan bnAbs, viral neutralization clade specificity and intra-clade breadth of DH270 depended primarily on the frequency of the N332 glycosylation site within the relevant clade (Fig, 2A). Only one of 62 pseudoviruses tested that lacked the PNG site at N332, the B clade virus 5768.04, was sensitive to DH270.5 and DH270.6 (Fig. 33). Across the full M group HIV-1 virus isolate panel used in neutrali zation assays, the loss of the PNG N332 sites accounted for 70% of the observed neutralization resistance. The circulating recombinant form CRFOl very rarely has this glycosylation site (3% of sequences in the Los Alamos database and 4% (1/23) in our test panel) and DH270 lineage antibodies did not neutralize CRFOl strains (Fig. 2A). As a consequence of the N332 PNG site requirement of V3 glycan bnAbs to neutralize, in vitro estimation of neutralizing breadth was impacted simply by the fraction of CRF01 viruses included in the panel . Other V3-glycan bnAbs (10- 1074, PGT121 and PGT128) shared this N332 glycan dependency but PGT121 and PGT128 were not as restrictive (Fig. 33) (5, 6, 8), Anti body 10-1074 was similar to DH270.6 in that it more strictly required the N332 PNG site, and its neutralization potency correlated with that of D! 1270 b (Pearson ' s p = 8.0c ¹ \ r = 0.63) (8).

[0182] Heterologous breadth and potency of DH270 lineage antibodies increased with accumulation of VH mutations and although DH270.UCA did not neutralize heterologous HIV- 1 , five amino-acid substitutions in DH270.LA.4 (four in the heavy chain, one in the light chain) were sufficient to initiate the bnAb lineage and confer heterologous neutralization (Figs. 2B, C and Figs. 34-35).

[0183] The capacity of the early DH270 lineage members to neutralize heterologous viruses correlated with the presence of short VI loops (Fig. 2D). As the lineage evolved, it gained capacity to neutralize viruses with longer VI loops, although with reduced potency (Fig. 2D and Figs. 16A-C). Neutralization of the same virus panel by V3 glycan bnAbs 10-1074, PGT121 and PGT128 followed the same inverse correlation between potency and VI length (Figs. 16D-F).

[0184] Mutations in the DH270 antibody lineage that initiated heterologous neutralization

[0185] The likelihood of AID-generated somatic mutation in immunoglobulin genes has strong nucleotide-sequence dependence (20)(21). Moreover, we have recently shown for CD4bs bnAbs that VH sites of high intrinsic mutability indeed determine many sites of somatic hypermutation (11). Like the VRCOl -class CD4bs bnAbs, both DH270 and DH272 used VH 1 -2*02 although unlike the CD4bs bnAbs, V3 glycan bnAbs in general can use quite disparate VH gene segments (3, 17, 22-25), and antibodies in both lineages have mutations at the same amino acid positions that correspond to sites of intrinsic mutability that we identified in the VH1-2*02 CD4bs bnAbs (/ /) (Fig. 17A). In HIV-1 negative individuals, we identified 20 aa that frequently mutate from the VH 1-2*02 germiine sequence (Fig. 17A). Twelve of these 20 aa were also frequently mutated in DH270 lineage antibodies and 1 1 of these 12 aa mutated to one of the two most frequent aa mutated in non-HTV-l VH1-2*02 sequences (identity conformity). G57R was the lone exception. DH272 mutated in 6 of these 12 positions and CD4bs bnAb VRCOl mutated in 1 1 out of 12 positions (Fig. 17A).

[0186] Presence of the canonical VH1-2*02 allele in individual CH848 was confirmed by genomic DNA sequencing (Fig. 17B). Four nucleotide changes in the 1)1 1270 UCA conferred heterologous neutralization activity to the next intermediate antibody (IA4). The G92A and G102A. nucleotide mutations in DH270.IA4 (and in DH272) occurred at "canonical" AID hotspots (DGYW) and encoded amino acid substitutions G31 D and M34I, respectively (Fig. 3A). G164C (G164A for DH272) was in a "non-canonical" AID hotspot with a comparable level of mutability (20) and encoded the S55T (N for DH272) substitution (Fig. 3A). In contrast, the G169C mutation in DH270.IA4, which encoded the G57R amino acid mutation, occurred at a site with a very low predicted level of mutabi lity (20), generated a canonical cold spot (GTC) and disrupted the overlapping AID hotspot at G170 within the same codon, which was instead used by DH272 and resulted in the G57V substitution (Fig. 3 A). Thus, while both the DH270 bnAb and DH272 autologous neutralizing lineages had mutations at Gly57, the substitution in the DH270 lineage (G57R) was an improbable event whereas the substitution (G57V) in the DH272 lineage was much more probable.

[0187] The G3 ID and M31I substitutions that occurred in AID hotspots became fixed in both lineages and S55T eventually became prevalent also in the DH272 lineage (Fig. 3B). By week 1 1 1 post-transmission, all DH272 lineage VHDJH transcripts sequenced by NGS harbored a mutation in the Gly57 codon, which resulted in the predominance of an encoded aspartic acid (Fig. 3B). In contrast, only 6/758 (0.8%) DH270 lineage transcripts isolated 186 weeks post-transmission had Val57 or Asp57; 48/758 (6,3%) retained Gly 57, whi le over two- thirds, 514/758 (67.8%), had G57R (Fig. 3B).

[0188] Since the rare G169C nucleotide mutation in DH270.IA4 introduced a cold spot and simultaneously disrupted the overlapping AID hotspot, it had a high probability once it occurred of being maintained, and indeed it was present in 523/758 (68%) DH270 lineage VH sequences identified with NGS at week 186 post-transmission (Fig. 3C).

[0189] Reversion of Arg57 to Gly abrogated DH270.IA4 neutralization of autologous and heterologous HIV-1 i solates (Fig. 3D). A DH270.IA4 R57V mutant, with the base change that would have occurred had the overlapping AID hotspot been used, also greatly reduced DH270. IA4 neutralization, confirming that Arg57, rather than the absence of Gly 57 was responsible for the acquired neutralizing activity (Fig. 3D). Finally, the DH270.UCA G57R mutant neutralized both autologous and heterologous viruses, confirming that G57R alone could confer neutralizing activity on the DH270 germline antibody (Fig. 3E). Thus, the improbable G169C mutation conferred reactivity against autologous virus and initiated acquisition of heterologous neutralization breadth in the DH270 lineage.

[0190] A search for an Env that might select for the critical G57R mutation in DH270 UCA or IA4-iike antibodies yielded Env 10.17 from week 135 of infection (Figs. 18 A, B), which derived from the only autologous virus Env that DH270.IA4 could bind. DH270.IA4 binding to Env 10.17 depended on presence of Arg57 and reversion of R57G was necessary and sufficient to abrogate binding (Fig. 18 A). Also, binding to Env 10.17 was acquired by DH270.UCA upon introduction of the G57R mutation (Fig. 18B).

[0191] Autologous neutralizing antibody lineages that cooperated with DH270

[0192] Evidence for functional interaction among the three N332-dependent lineages came from the respective neutralization profiles against a panel of 90 autologous viruses from transmitted/founder to week 240 post-transmission (Fig. 4A and Figs. 34-35). Both DH475 and DH272 neutralized autologous viruses isolated during the first year of infection that were resistant to most DH270 lineage antibodies (only DH270.IA1 and DH270.4 neutralized weakly) (Fig. 4 A). DH475 neutralized viruses from week 15 through week 39 and DH272 neutralized the CH848 transmitted/founder and all viruses isolated up to week 51 , when viruses that resisted DH475 and DH272 became strongly sensitive to the more mature antibodies in the DH270 lineage (V^'H iit mutation frequency > 5.6%) (Fig. 4A).

[0193] The identification of specific mutations implicated in the switch of virus sensitivity was complicated by the high levels of mutations accumulated by virus Env over time (Fig. 19 and Fig. 36). We identified virus signatures that defined the DH270.1 and DH272/DH475 immunotypes and introduced four of them, in various combinations, into the DH272/DH475- sensitive virus that was closest in sequence to the DH270.1 -sensitive immunotype: a 10 amino-acid residue deletion in VI (Δ134-143); a D 185N mutation in V2, which introduced an N-linked glycosylation site; an N413Y mutation in V4, which disrupted an N-iinked glycosylation site; and a 2 amino-acid residue deletion (Δ4. 63-464) in V5.

[0194] The large V deletion was critical for DH270.1 neutralization, with smaller contributions from the other changes; the VI deletion increased virus resistance to DH475 (3.5-fold increase), VI -loop-mediated resistance to DH475 neutralization increased further when combined with the Δ463-464 V5 deletion (5-fold increase) (Fig. 4B).

[0195] The VI loop of the transmitted/founder vims (34 residues) was longer than the average VI length of 28 residues (range 1 1 to 64) of HIV- 1 Env sequences found in the Los Alamos Sequence Database (26). As we found for heterologous neutralization, DH270 lineage antibodies acquired the ability to neutralize larger fractions of autologous viruses as maturation progressed by gaining activity for viruses with longer VI loops, although at the expense of lower potency (Figs. 20A-C). This correlation was less clear for gpl20 binding (Figs. 20D-F), however, suggesting that the VI loop-length dependency of V3 glycan bnAb neutralization has a conformational component. Thus, DH475 cooperated with the DH270 bnAb lineage by selecting viral escape mutants sensitive to bnAb lineage members. [0196] For DH272, the viral variants that we made did not implicate a specific cooperating escape mutation. The Δ134-143 (VI deletion) mutated virus remained sensitive to 1)1 1272 neutralization; both combinations of the VI deletion in our panel that were resistant to DH272 and sensitive to DH270.1 included D 185N, which on its own also caused DH272 resistance but did not lead to DH270.1 sensitivity (Fig. 4C). Thus, we have suggestive, but not definitive, evidence that DH272 also participated in selecting escape mutants for the DH270 bnAb lineage.

[0197] Structure of DH270 lineage members

[0198] We determined crystal structures for the single-chain variable fragment of DH270.1 and the Fabs of DH270.UCA3, DH270.3, DH270.5 and 1)1 1270.6, as well as for 1)1 1272 (Fig. 32). Because of uncertainty in the inferred sequence of the germline precursor (Figs. 21 A, B), we also determined the structure of DH270.UCA1, which has a somewhat differently configured CDR H3 loop (Fig. 21 C); reconfiguration of this loop during early affinity maturation could account for the observed increase with respect to the UCA in heterologous neutralization by several intermediates. The variable domains of the DH270 antibodies superposed well, indicating that affinity maturation modulated the antibody-antigen interface without substantially changing the antibody conformation (Fig. 5 A). Mutations accumulated at different positions for DH270 lineage bnAbs in distinct branches (Fig. 22), possibly accounting for their distinct neutralization properties, DH272 had a CDRH3 configured differently from that of DH270 lineage members and a significantly longer CDRL l (Fig. 5B), compatible with their distinct neutralization profiles.

[0199] We also compared the structures of DH270 lineage members with those of other N332-dependent bnAbs. All appear to have one long CDR loop that can extend through the network of glycans on the surface of the gp l 20 subunit and contact the "shielded" protein surface. The lateral surfaces of the Fab variable module can then interact with the

reconfigured or displaced glycans to either side, PGT128 has a long CDRH2 (Fig. 5C), in which a 6-residue insertion is critical for neutralization breadth and potency (J, 17). PGT124 has a shorter and differently configured CDR H2 loop, but a long CDR H3 instead (Fig. 5D) (27).

[0200] Structure of the DFI270 - HIV Env Complex

[0201 ] We determined a three-dimensional (3D) image reconstruction, from negative- stain electron microscopy (EM), of the Dl 1270, 1 Fab bound with a gp l40 trimer (92Br SOSIP.664) (Figs. 5E, F and Figs. 23 A-B). The three DH270.1 Fabs project laterally, with their axes nearly normal to the threefold of gpl40, in a distinctly more "horizontal" orientation than seen for PGT124, PGT135 and PGT128 (Figs, 5G, H and Fig. 24). This orientational difference is consistent with differences between DH270 and PGT124 or PGT1.28 in the lengths and configurations of their CDR loops, which required an alternative DH270 bnAb position when docked onto the surface of the Env trimer. We docked the BG505 SOSIP coordinates (28) and the Fab into the EM reconstruction, and further constrained the EM reconstruction image by the observed effects of BG505 SOSIP mutations in the gpl4(⁾ surface image (Figs. 23 A-B and Figs. 25A-B). Asp325 was essential for binding

DH270.1 since it is a potential partner for Arg57 on the Fab. Mutating Asp321 led to a modest loss in affinity; R327A had no effect (Fig. 26A-C). These data further distinguish DH270 from PGT124 and PGT128. Mutating W101, Y105, D107, Dl 15, Yl 16 or Wl 17 in

DH270.1 individual ly to alanine substantially reduced binding to the SOSIP trimer, as did pairwise mutation to alanines of S I 06 and S I 09. The effects of these mutations illustrate the critical role of the CDRH3 loop in binding with HIV-1 Env (Figs. 26A-C).

[0202] DH270 UCA binding

[0203] The DH270 UCA did not bind to any of the 120 CH848 autologous gpl 20 Env glycoproteins isolated from time of infection to 245 weeks post-infection, including the TF Env (Fig. 6 A). DH270 UCA, as well as maturation intermediate antibodies, also did not recognize free glycans or ceil surface membrane expressed gp!60 trimers (Fig. 6B).

Conversely, the DH270 UC A bound to the Man9-V3 synthetic glycopeptide mimic of the V3- glycan bnAb gpl20 epitope (Fig. 27 A) and also bound to the aglycone form of the same peptide (Fig. 27B). Similarly, the early intermediate antibodies (IA4, IA3, IA2) each bound to both the Man9-V3 glycopeptide and its aglycone form, and their binding was stronger to the aglycone V3 peptide than to the Man9-V3 glycopeptide (Fig. 27B). Overall, DH270 UCA and early intermediate antibodies binding to the Man9-V3 glycopeptide was low (>10μΜ) (Fig. 27A). DH270.1 (VH nt mutation frequency: 5.6%) bound the glycopeptide with higher affinity than did the aglycone (Kd,giycopepude ^:;= 331nM) (Figs. 27 A, B) and, as mutations accumulated, binding of the Man9-V3 glycopeptide also increased, culminating in a ·.ι of 188 nM in the most potent bnAb, DFI270.6, which did not bind to the aglycone- V3 peptide (Figs. 27A, B). Thus, both the Man9-V3 glycopeptide and the aglycone- V3 peptide bound to the DH270 UCA, and antibody binding was independent of glycans until the DH270 lineage had acquired a nucleotide mutation frequency of ~6%.

[0204] Discussion [0205] We can reconstruct from the data presented here a plausible series of events during the development of a V3-glycan bnAb in a natural infection. The DH272 and DH475 lineages neutralized the autologous TF and early viruses, and the resulting escape viruses were neutralized by the DH270 lineage. In particular, VI deletions were necessary for neutralization of all but the most mature DH270 lineage antibodies. DH475 (and possibly DH272) escape variants stimulated DH270 affinity maturation, including both somatic mutations at sites of intrinsic mutability (77) and a crucial, improbable mutation at an AID coldspot within CDRH2 (G57R). The G57R mutation initiated expansion of the DH270 bnAb lineage. The low probability of this heterologous neutralization-conferring mutation and the complex lineage interactions that occurred is one explanation for why it took 4.5 years for the Dl 1270 lineage to expand.

[0206] The CH848 viral population underwent a transition from a long VI loop in the TF (34 residues) to short loops (16-17 residues) when escaping DH272/DH475 and facilitating expansion of DH270, to restoration of longer VI loops later in infection as resistance to DH270 intermediates developed. Later DH270 antibodies adapted to viruses with longer VI loops, allowing recognition of a broader spectrum of Envs and enhancing breadth, DH270.6 could neutralize heterologous viruses regardless of VI loop length, but viruses with long loops tended to be less sensitive to it. Association of long VI loops with reduced sensitivity was evident for three other V3 glycan bnAbs isolated from other individuals and may be a general feature of this class.

[0207] The VI loop deletions in CH848 autologous vims removed the PNG site at position 137. While the hypervariable nature of the VI loop (which evolves by insertion and deletion, resulting in extreme length heterogeneity, as well as extreme variation in number of PNG sites) complicates the interpretation of direct comparisons among unrelated HIV-1 strains, it is worth noting that a PNG in this region specified as N 137 was shown to be important for regulating affinity maturation of the PGT121 V3 glycan bnAb family, with some members of the lineage evolving to bind (PGT121 -123) and others (PGT124) to accommodate or avoid this glycan (29).

[0208] Since we cannot foresee the susceptibility to a particular bnAb lineage of each specific potential transmitted/founder virus to which vaccine recipients will be exposed, it will be important for a vaccine to induce bnAbs against multiple epitopes on the HIV- 1 Env to minimize transmitted/founder vims escape (30, 31). In particular, induction of bnAb specificities beyond the HIV-1 V3 glycan epitope is critical for use in Asian populations where CRF01 strains, which lack for the most part the N332 PNG required for efficient neutralization by V3 glycan bnAbs, is frequently observed.

[0209] Regarding what might have stimulated the DC A of the DH270 bnAb lineage, the absence of detectable binding to the CH848 TF Env raised at least two possibilities. One is that the lineage arose at the end of year 1 , either from a primary response to viruses present at that time (e.g., with deletions in VI -V2) or from subversion of an antibody lineage initially elicited by some other antigen. The other is that some altered form of the CH848 TF envelope protein (e.g. shed gpl20, or a fragment of it) exposed the V3 loop and the N301 and N332 glycans in a way that bound and stimulated the germline BCR, even though the native CH848 TF Env did not. Our findings suggest that a denatured, fragmented or otherwise modified form of Env may have initiated the DH270 lineage. We cannot exclude that the DH270 UCA could not bind to autologous Env as an IgG but could potentially be triggered as an IgM B ceil receptor (BCR) on a ceil surface.

[0210] It will be important to define how often an improbable mutation such as G57R determines the time it takes for a bnAb lineage in an FflV-1 infected individual to develop, and how many of the accompanying mutations are necessary for potency or breadth rather than being non-essential mutations at AID mutational hotspots (11, 32). Mutations of the latter type might condition the outcome or modulate the impact of a key, improbable mutation, without contributing directly to affinity. Should the occurrence of an unlikely mutation be rate-limiting for breadth or potency in many other cases, a program of rational immunogen design will need to focus on modified envelopes most likely to select very strongly for improbable yet critical antibody nucleotide changes

[0211] The following proposal for a strategy to induce V3 glycan bnAbs recreates the events that led to bnAb induction in CH848: start by priming with a ligand that binds the bnAb UCA, such as the synthetic glycopeptide mimic of the V3 -glycan bnAb gpl20 epitope, then boost with an Env that can select G57R CDR H2 mutants, followed by Envs with progressive VI lengths (Fig, 28). We hypothesize that more direct targeting of V3 -glycan UCAs and intermediate antibodies can accelerate the time of V3 -glycan bnAb development in the setting of vaccination.

[0212] A limitation of this approach is that the selection of immunogens was based on the analysis of a single lineage from a single individual and how frequently DH270-like lineages are present in the general population is unknown. Finally, our study describes a general strategy for the design of vaccine immunogens that can select specific antibody mutations thereby directing antibody lineage maturation pathways. [0213] MATERIAL AND METHODS

[0214] Study Design, The CH848 donor, from which the DH270, DH272 and DH475 antibody lineages were isolated, is an African male enrol led in the CHAVIOOl acute HIV-1 infection cohort (33) and followed for 5 years, after which he started antiretroviral therapy. During this time viral load ranged from 8,927 to 442,749 copies/ml (median = 61,064 copies/mi), and CD4 counts ranged from 288 to 624 cells/mm^ (median = 350 cells/ mm³). The time of infection was estimated by analyzing the sequence diversity in the first available sample using the Poisson Fitter tool as described in (10). Results were consistent with a single founder virus establishing the infection (34),

[0215] MAbs DH270.1 and DH270.3 were isolated from cultured memory B cells isolated 205 weeks post-transmission (14). DH270.6 and DH475 mAbs were isolated from Man9-V3 glycopeptide-specific memory B cells col lected 232 and 234 weeks post-transmission, respectively, using direct sorting. DH270.2, DH270.4 and DH270.5 mAbs were isolated from memory B cells collected 232 weeks post-transmission that bound to Consensus C gpl20 Env but not to Consensus C N332A gp^'120 Env using direct sorting

[0216] Statistical Analyses, Stati stical analysis was performed using R. The specific tests used to determine significance are reported for each instance in the text.

[0217] Flow Cytometry, Memory B Cell Cultures and mAb Isolation

[0218] A total of 30,700 memory B cells from individual CH848 were isolated from PBMC collected 205 weeks post-transmission using magnetic-activated cell sorting as described in (14). Memory B cells were cultured at limiting dilution at a calculated concentration of 2 cells/well for 2 weeks as described in (1 1) using irradiated CD40L L cells (7,500 c-Gy) as feeder cel ls at a concentration of 5,000 cell s/well , culture medium was refreshed 7 days after plating. Cell culture supematants were screened for neutralization of autologous CH848.TF virus using the tzm-bl neutralization assay (14) and for binding to CH848.TF gpl2(⁾ Env, CH848.TF gpl40 Env, Consensus C gp^'120 Env and consensus C N332A gp^'120 Env, Concurrently, cel ls from each culture were transferred in RNAlater (Qiagen) and stored at - 80°C until functional assays were completed.

[0219] MAbs Pi 1270. 1 and DH270.3 were isolated from cultures that bound to CFI848.TF gp l 20 Env and Consensus C gpl20 but did not bind to C N332A gp l 20 Env. DH272 was isolated from a culture that neutralized 99% CH848.TF virus infectivity. Dl 1272 dependency to N332-linked glycans was first detected on the transiently transfected recombinant antibody tested at higher concentration and confirmed in the purified recombinant antibody. From the stored RNAlater samples, mRNA of cells from these cultures was extracted and retrotran scribed as previously described (14).

[0220] DH270.6 and DH475 mAbs were isolated from Man9~V3 glycopepti de-specific memory B cells collected 232 and 234 weeks post-transmission, respectively, using direct sorting (16). Briefly, biotinylated Man9-V3 peptides were tetramerized via streptavidin that was conjugated with either AlexaFluor 647 (AF647; ThermoScientific) or Brilliant Violet 421 (BV421) (Biolegend) dyes. Peptide tetramer quality following conjugation was assessed by flow cytometry to a panel of well-characterized 1 ί ί V - 1 V3 glycan antibodies (PGT128, and 2G12) and linear V3 antibodies (F39F) attached to polymer beads. PBMCs from donor CH848 were stained with LIVE/DEAD Fixable Aqua Stain (ThermoScientific), anti-human IgM (FITC), CD3 (PE-Cy5), CD235a (PE-Cy5), CDI9 (APC-Cy7), and CD27 (PE-Cy7) (BD Biosciences); anti-human antibodies against IgD (PE); anti-human antibodies against CD10 (ECD), CD38 (APC-AF700), CD19 (APC-Cy7), CD16 (BV570), CD14 (BV605)

(Biolegend); and Man9GlcNac2 V3 tetramer in both AF647 and BV421 , PBMCs that were Aqua Stain -, CD14-, CD 16-, CD3-, CD235a-, positive for CD 19+, and negative for surface IgD were defined as memory B cells; these cells were then gated for Man9-V3+ positivity in both AF647 and BV421 , and were single-cell sorted using a BD FACS Aria II into 96-well plates containing 20μ1 of reverse transcriptase buffer (RT).

[0221] DH270.2, DH270.4 and DH270.5 mAbs were isolated from memory B cells collected 232 weeks post-transmission that bound to Consensus C gpl20 Env but not to Consensus C N332A gpl20 Env using direct sorting. Reagents were made using biotinylated Consensus C gpl20 Env and Consensus C N332A gp!20 Env by reaction with streptavidin that was conjugated with either AlexaFluor 647 (AF647; ThermoScientific) or Brilliant Violet 421 (BV421) (Biolegend) dyes, respectively. Env tetramer quality following conjugation was assessed by flow cy tometry to a panel of well-characterized HIV-1 V3 glycan antibodies (PGT128, and 2G12) and linear V3 antibodies (F39F) attached to polymer beads. PBMCs were stained as outlined for DH475 and DH270.6, however these cells were then gated for Consensus C gpl20 positivity and Consensus C N332A gpl20 negativity in AF647 and BV421, respectively, and were single cell sorted and processed as outlined for DH475 and DH270.6.

[0222] For all antibodies, cDNA synthesis, PGR amplification, sequencing and V(D)J rearrangement analysis were conducted as previously described (1 1). Reported mutation frequency is calculated as frequency of nucleotide mutations in the V gene region of antibody sequence. CDRH3 lengths reported are defined as the number of residues after the invariant Cys in FR3 and before the invariant Trp in FR4.

[0223] Antibody production

[0224] Immunoglobulin genes of mAbs DH270.1 through DH270.6, DH272 and DH475 were amplified from RNA from isolated cells, expression cassettes made, and mAbs expressed as described (12, 14). Inference of unmutated common ancestor (UCA) and intermediate antibodies DH270. LA through DH270.IA.4 was conducted using methods previously described (36).

[0225] Heavy chain plasmids were co-transfected with appropriate light chain plasmids at an equal ratio in Expi 293 cells using ExpiFectamine 293 transfection reagents (Thermo Fisher Scientific) according to the manufacturer' s protocols. We used the enhancer provided with the kit, transfected cultures were incubated at 37°C 8% C02 for 2-6 days, harvested, concentrated and incubated overnight with Protein A beads at 4°C on a rotating shaker before loading the bead mixture in columns for purification; following PBS/NaCl wash, eluate was neutralized with trizma hydrochloride and antibody concentration was determined by ^'Nanodrop. Purified antibodies were tested in SDS-Page Coomassie and western blots, and stored at 4°C.

[0226] Next-generation sequencing

[0227] PBMC -extracted RNA from weeks 1 1, 19, 64, 1 1 1 , 160, 186, and 240 post-infection were used to generate cDNA amplicons for next-generation sequencing (Illumina Miseq) as described previously (35). Briefly, RNA isolated from PBMCs was separated into two equal aliquots before cDNA production; cDNA amplification and NGS were performed on both aliquots as independent samples (denoted A and B), Reverse transcription (RT) was carried out using human IgG, Ig A, IgM, IgK and Igk primers as previously described (12). After cDNA synthesis, IgG isotype IGHV1 and IGHV3 genes were amplified separately from weeks 1 1, 19, 64, 1 1 1 , 160, and 186, IGHV 1-IGHY6 genes were amplified at week 240. A second PGR step was performed to add Nextera index sequencing adapters (Illumina) and libraries were purified by gel extraction (Qiagen ) and quantified by quantitative PGR using the KAPA SYBR FAST qPCR kit (KAPA Biosystems). Each replicate library was sequencing using the Illumina Miseq V3 2x 300bp kit.

[0228] NGS reads were computationally processed and analyzed as previously described (35). Briefly, forward and reverse reads were merged with FLASH with average read length and fragment read length parameters set to 450 and 300, respectively. Reads were quality filtered using FASTX (http://hannonlabxshl.edu/fastx_toolkit/) for sequences with a minimum of 50 percent of bases with a Phred quality score of 20 or greater (corresponding to 99% base call accuracy). Primer sequences were discarded and only unique nucleotide sequences were retained. To mitigate errors introduced during PCR amplification, reads detected in sample A and B with identical nucleotide VHDJH rearrangement sequences were delineated as replicated sequences. The total number of unique reads per sample and total number of replicated sequences ("Overlap") across samples for each time point is listed in Fig. 30. We used replicated sequences to define presence of antibody clonal lineages at any time-point.

[0229] We identified clonally-related sequences to DH270, DH272 and DH475 from the longitudinal NGS datasets by the following procedure. First, the CDR H3 of the probe- identified clonal parent sequence was BLASTed (E-value cutoff =.01 ) against the pooled sample A and B sequence sets at each timepoint to get a candidate set of putative clonal members ("candidate set"). Next we identified replicated sequences across samples A and B in the candidate set. We then performed a clonal kinship test with the Cloanalyst software package (http://www.bu.edu/computationalimmunology/research/software/) as previously described (35) on replicated sequences. Clonally-related sequences within Sample A and B (including non-replicated sequences) were identified by performing the same clonal kinship test with Cloanalyst on the candidate set prior to identifying replicated sequences.

[0230] Clonal lineage reconstruction was performed on the NGS replicated sequences and probe-identified sequences of each clone using the Cloanalyst software package. A maximum of 100 sequences were used as input for inferring phylogenetic trees of clonal lineages. Clonal sequence sets were sub-sampled down to 100 sequences by collapsing to one sequence within a 2 or 9 base pair difference radius for the DH272 and DH270 clones, respectively.

[0231] The pre-vaccination NGS samples that were analyzed in Fig. 17A were obtained from HIV-1 uninfected participants of the HVTN082 and HVTN204 trials as previously described (35).

[0232] Sequence Analysis of Antibody Clonal Lineages

[0233] Unmutated common ancestors (UCA) and ancestral intermediate sequences were computationally inferred with the Cloanalyst software package, Cloanalyst uses Bayesian inference methods to infer the full unmutated V(D)J rearrangement thereby including a predicted unmutated CDR3 sequence. For lineage reconstructions when only cultured or sorted sequences were used as input, the heavy and light chain pairing relationship was retained during the inference of ancestral sequences. UCA inferences were performed each time a new member of the Dl 1270 clonal lineage was experimentally isolated and thus several versions of the DH270 UCA were produced and tested, UCA1 and UCA3 were used for structural determination. UCA4 (referred to as DH270.UCA throughout the text), which was inferred using the most observed DH270 clonal members and had the lowest uncertainty of UCAs inferred (as quantified by the sum of the error probability over all base positions in the sequence), was used for binding and neutralization studies. Subsequently, the DH270 UCA was also re-inferred when NGS data became available. We applied a bootstrapping procedure to infer the UCA with the NGS data included, resampling clonal lineage trees 10 times with 100 input NGS sequences each. The UCA4 amino acid sequence was

recapitulated by 7 out of 10 UCA inferences of the resampled NGS trees confirming support

[0234] Each inference of V(D)J calls is associated with a probability. The probability of the DH270 lineage to use the VH1-2 family gene was 99.99% and that of using allele 02 (VH1 - 2*02) was 98.26%. Therefore, there was a 0.01% probability that the family was incorrectly identified and a 1 ,74% probability that the allele was incorrectly identified. Therefore, we sequenced genomic DNA of individual CH848. As previously reported, positional conformity is defined as sharing a mutation at the same position in the V gene segment and identity conformity as sharing the same amino acid substitution at the same position ( 1).

[0235] We refer to the widely established AID hot and cold spots (respectively WRCY and SYC and their reverse-complements) as "canonical" and to other hot and cold spots defined by Yaari et al. as "non-canonical" (20, 37-39).

[0236] Sequencing of germline variable region from genomic DNA

[0237] Genomic DNA was isolated from donor CH848 from PBMCs 3 weeks after infection (QIAmp DNA Blood mini kit; Qiagen). IGV 1-2 and IGVL2-23 sequences were amplified using 2 independent primer sets by PGR. To ensure amplification of non-rearranged variable sequences, both primer sets reverse primers aligned to sequences present in the non-coding genomic DNA downstream the V-recombination site. The forward primer for set 1 resided in the IGVH1-2 and IGVL2-23 leader sequences and upstream of the leader in set 2. The PGR fragments were cloned into a pcDNA2.1 (TOPO-TA kit; Life technologies) and transformed into bacteria for sequencing of individual colonies. The following primers were used: VH1- 2__1__S: tcctcttcttggtggcagcag; VH1 -2_2_S: tacagatctgtcctgtgccct; VHl-2_l_tmAS:

ttctcagccccagcacagctg; VEQ -2 2 TmAS : gggtggcagagtgagactctgtcaca; VL2-23_2_S : agaggagcccaggatgctgat; VL2-23_1_S: actctcctcactcaggacaca; VL2-23_1_AS: tctcaaggccgcgctgcagca; VL2-23 _2_AS : agctgtccctgtcctggatgg.

[0238] We identified two variants of VH 1-2*02: the canonical sequence and a variant that encoded a VH that differed by 9 amino acids. Of these 9 amino acids, only 1 was shared among DH270 antibodies whereas 8 amino acids were not represented in DH270 lineage antibodies (Fig. 17B). The VH 1-2*02 variant isolated from genomic DNA did not encode an arginine at position 57. We conclude that between the two variants of VH 1-2*02 identified from genomic DNA from this individual, the DH270 lineage is likely derived from the canonical VHl-2*02 sequence.

[0239] Direct binding ELISA

[0240] Direct-binding ELISAs were performed as described (11). Briefly, 384-well plates were blocked for 1 h at room temperature (RT) or overnight at 4°C (both procedures were previously validated); primary purified antibodies were tested at a starting concentrations of l()()|ig/ml, serially three-fold diluted and incubated for 1 h at RT; HRP-conjugated human IgG antibody was added at optimized concentration of 1 :30,000 in assay diluent for 1 hour and developed using TMB substrate; plates were read at 450 nm in a SpectraMax 384 PLUS reader (Molecular Devices, Sunnyvale, CA); results are reported as logarithm area under the curve (LogAUC) unless otherwise noted.

[0241] For biotinylated avi -tagged antigens, plates were coated with streptavidin ^g/ml); blocked plates were stored at -20°C until used and biotinylated avi -tagged antigens were added at 2 μg/mi for 30 minutes at RT.

[0242] Competition ELI SAs were performed using Ι ΟμΙ of primary purified monoclonal antibody, starting at

and diluted in a two-fold concentration, incubated for 1 h at RT. Ten μΐ of biotinylated target Mab was added at the EC50 determined by a direct binding of biotinylated-Mab for one hour at RT. After background subtractions, percent inhibition was calculated as follows: 100-(test Ab triplicate mean/no inhibition control mean)* 100.

[0243] A ssessment of vims neutralization

[0244] Antibody and plasma neutralization was measured in TZM-bl cell-based assays. Neutralization breadth of DH270.1, DH270.5 and DFI270.6 was assessed using the 384-well plate declination of the assay using an updated panel of 207 geographically and genetically diverse Env-pseudoviruses representing the major circulating genetic subtypes and recombinant forms as described (40). The data were calculated as a reduction in

luminescence units compared with control wells, and reported as IC50 in μg/ml.

[0245] Single genome sequencing and pseudovirus production [0246] 3' half genome single genome sequencing of HIV- 1 from longitudinally collected plasma was performed as previously described (41, 42). Sequence alignment was performed using ClustalW (version 2. 1 1 ) and was adjusted manually using Geneious 8 (version 8.1 ,6). Env amino acid sequences were then aligned and evaluated for sites under selection using code derived from the Longitudinal Antigenic Sequences and Sites from Intra-host Evolution (LASSIE) tool (43). Using both LASSIE-based analysis and visual inspection, 100 representative env genes were selected for pseudovirus production. CMV promoter-ligated env genes were prepared and used to generate pseudotyped viruses as previously described (44).

[0247] Generation of cell surface-expressed CH848 Env trimer CHO ceil line

[0248] The membrane-anchored CH848 TF Env trimer was expressed in CHO-S cells.

Briefly, the CH848 env sequence was codon-optimized and cloned into an HIV-l-based ientiviral vector. A heterologous signal sequence from CDS was inserted replacing that of the HIV-1 Env. The proteolytic cleavage site between gpl 20 and gp41 was altered, substituting serine residues for Arg508 and ArgS l 1, the tyrosine at residues 712 was changed to alanine (Y712A), and the cytoplasmic tail was truncated by replacing the Lys808 codon with a sequence encoding (Giy)3 (His)6 followed immediately by a TAA stop codon. This env- containing sequences was inserted into the vector immediately downstream of the

tetracycline (tet)-responsive element (TRE), and upstream of an internal ribosome entry site (IRES) and a contiguous puromyciti (puro)-T2A-EGFP open reading frame (generating K4831), as described previously for the JRFL and CH505 Envs (45).

[0249] CHO-S cells (Invitrogen) modified to constitutively express the reverse tet

transact! vator (rtTA) were transduced with packaged vesicular stomatitis virus (VSV) G glycoprotein-pseudotyped CH848 Env expression vector. Transduced cells were incubated in culture medium containing l ig/ml of doxycycline (dox) and selected for 7 days in medium supplemented with 25 ^tg/mi of puromycin, generating the Env expressor-popuiation cell line termed D831 . From D831 , a stable, high-expressor clonal cell line was derived, tenned D835. The integrity of the recombinant env sequence in the clonal cell lines was confirmed by direct (without cloning) sequence analysis of PGR ampl icons.

[0250] Cell surface-expressed trimeric CH848 Env binding

[0251] D831 Selected TRE2.CH848.JF-8.IRS6A Chinese Hamster Ovary Ceils were cultured in DMEM/F-12 supplemented with HEPES and L-glutamine (Thermo Fischer,

Cat#l 1330057) 10% heat inactivated fetal bovine serum [FBS] (Thermo Fischer,

Cat#10082147) and 1% Penicillin-Streptomycin (Thermo Fischer, Cat#15140163) and harvested when 70-80% confluent by trypsinization. A total 75,000 viable cells/well were transferred in 24-well tissue culture plates. After a 24-to-30-hour incubation at 37°C/5% C02 in humidified atmosphere, CH848 Envs expression was induced with l .ug/mL doxycycline (Sigma-Aldrich, Cat#D9891) treatment for 16-20 hours. Ceils were then washed in Stain buffer [PBS/2% FBS] and incubated at 4°C for 30 minutes. Stain buffer was removed from cells and 0.2ml/well of DH270 lineage antibodies, palivizumab (negative control) or PGT128 (positive control) were added at optimal concentration of Sfig/mL for 30 minutes at 4°C. After a 2X wash, cells were stained with 40 ul of APC-conjugated mouse anti-Human IgG (BD Pharmigen, Cat#562025) per well (final volume 0.2 ml/well) for 30 minutes at 4°C. Unstained cells were used as further negative control. Cells were washed 3X and gently dissociated with 0.3 ml/well PBS/5 mM EDTA for 30 minutes at 4°C, transfeired into 5mL Polystyrene Round-Bottom Tubes (Falcon, Cat#352054), fixed with 0, 1 mL of BD Cytofix/Cytoperm Fixation solution (BD Biosciences, Cat#554722) and kept on ice until analyzed using a BD LSRFortessa Cell Analyzer, Live cells were gated through

Forward/Side Scatter exclusion, and then gated upon GFP+ and APC.

[0252] Oligomannose Arrays

[0253] Oligomannose arrays were printed with glycans at 100, 33, and 10 μΜ (Z Biotech). Arrays were blocked for ih in Hydrazide glycan blocking buffer. Monoclonal antibodies were diluted to 50 g/mL in Hydrazide Glycan Assay Buffer, incubated on an individual sub array for 1 h, and then washed 5 times with PBS supplemented with 0.05% tween-20 (PBS-T). Sub arrays that received biotinylated Concanavalin A were incubated with streptavidin-Cy3 (Sigma), whereas all other wells were incubated with anti-IgG-Cy3 (Sigma) for Ih while rotating at 40 rpm covered from light. The arrays were washed 5 times with 70 ί of PBS-T and then washed once with 0.01X PBS. The washed arrays were spun dry and scanned with a GenePix 4000B (Molecular Devices) scanner at wavelength 532 rati using GenePix Pro7 software. The fluorescence within each feature was background subtracted using the local method in GenePix Pro? software (Molecular Devices). To determine glycan specific binding, the local background corrected fluorescence of the print buffer alone was subtracted from each feature containing a glycan.

[0254] Synthesis of Man9-V3 giycopeptide

[0255] A 30-amino acid V3 giycopeptide with oligomannose glycans (Man9~V3), based on the ciade B JRF^'L mini-V3 construct (16), was chemically synthesized as described earlier (18). Briefly, after the synthesis of the oligomannose glycans in solution phase (18), two partially protected peptide fragments were obtained by Fmoc-based solid phase peptide synthesis, each featuring a single unprotected aspartate residue. The Man9GlcNAc2 anomeric amine was conjugated to each fragment (D301 or D332) using our one-flask

aspartylation/deprotection protocol yielding the desired ^'N -linked glycopeptide. These two peptide fragments were then joined by native chemical ligation immediately followed by cyclization via disulfide formation to afford Man9-V3-biotin. The control peptide, aglycone V3-biotin, had identical amino acid sequence as its glycosylated counterpart.

[0256] Affinity measurements

[0257] Antibody binding kinetic rate constants (ka, kd) of the Man9-V3 glycopeptide and its aglycone form (16) were measured by Bio-layer Interferometry (BLI, FoiteBio Octet Red96) measurements. The BLI assay was performed using streptavidin coated sensors (FoiteBio) to capture either biotin-tagged Man9-V3 glycopeptide or Aglycone- V3 peptide. The V3 peptide immobilized sensors were dipped into varying concentrations of antibodies following blocking of sensors in BSA (0.1%). Antibody concentrations ranged from 0.5 to 150 .ug/mL and non-specific binding interactions were subtracted using the control anti-RSV

Palivizumab (Synagis) mAb. Rate constants were calculated by global curve fitting analyses to the Bivalent Avidity model of binding responses with a 10 rain association and 15 min dissociation interaction time. The dissociation constant (Kd) values without avidity contribution were derived using the initial components of the association and dissociation rates (kal and kdl) respectively. Steady-state binding Kd values for binding to Man9-V3 glycopeptide with avidity contribution were derived using near steady-state binding responses at varying antibody concentrations (0.5-80ug/mL) and using a non-linear 4-paramater curve fitting analysis.

[0258] HIV-1 Env site-directed mutagenesis

[0259] Deletion Mutant of CH0848.d0274.30.07 env gene was constructed using In Fusion HD EcoDry Cloning kit (Clontech) as per manufacturer instructions. Quick Change II Site- Directed Mutagenesis kit (Agilent Technologies) was used to introduce point mutations. Ail final env mutants were confirmed by sequencing.

[0260] Antibody site-directed mutagenesis

[0261] Site-directed mutagenesis of antibody genes was performed using the Quikchange II lightening multi-site-directed mutagenesis kit following manufacturer's protocol (Agilent). Mutant plasmid products were confirmed by single-colony sequencing. Primers used for introducing mutations were: DH270_IA4_D31G: cccagtgtatatagtagccggtgaaggtgtatcca;

DH270.IA4 I34M: tcgcacccagtgcatatagtagtcggtgaaggtgt; DH270.IA4 T55S:

gatggatcaaccctaactctggtcgcacaaactat; DH270.IA4 R57G: tgtgcatagtttgtgccaccagtgttagggttgat; DH270.IA4 R57V: cttctgtgcatagtttgtgacaccagtgttagggttgatc; DH270.UCA G57R:

atcaaccctaacagtggtcgcacaaactatgcaca.

[0262] Env glycoprotein expression

[0263] The codon-optimized CH848-derived env genes were generated by de novo synthesis (GeneScript, Piscataway, NJ) or site-directed mutagenesis in mammalian expression plasmid pcDNA3.1/hygromycin (Invitrogen) as described (10), and stored at ^~80°C until use.

[0264] Expression and purification of DH270 lineage members for crystallization studies [0265] The heavy- and light-chain variable and constant domains of the DH270 lineage Tabs were cloned into the pVRC-8400 expression vector using Notl and Nhel restriction sites and the tissue plasminogen activator signal sequence. The DH270.1 single chain variable fragment (scFv) was cloned into the same expression vector. The C terminus of the heavy- chain constructs and scFv contained a noncleavable 6x histidine tag. Site-directed mutagenesis was carried out, using manufacturer's protocols (Stratagene), to introduce mutations into the CDR regions of DH270.1 . Fabs were expressed and purified as described previously (46). The DH270.1 scFv was purified the same way as the Fabs.

[0266] Crystallization, structure determination, and refinement

[0267] All His-tagged Fabs and scFv were crystallized at 20-25mg/mL. Crystals were grown in 96-weli format using hanging drop vapor diffusion and appeared after 24-48 h at 20 °C. Crystals were obtained in the following conditions: 2.5M ammonium sulfate and lOOmM sodium acetate, pH 5.0 for DH272; 1.5M ammonium sulfate and lOOmM sodium acetate pH 4.0 for UCA1; 20% PEG 4K, lOOmM sodium acetate, pH 5 and l OOmM magnesium sulfate for UCA3; lOOmM sodium acetate, pH 4.5, 200mM lithium sulfate, and 2.5M NaCl for DH270.1; 1.4M lithium sulfate and l OOmM sodium acetate, pH 4.5 for DH270.3; 40% PEG 400 and lOOmM sodium citrate, pH 4.0 for DH270.5; and 30% PEG 4K, lOOmM PIPES pH 6, 1 M NaCl for DH270.6. All crystals were harvested and cryoprotected by the addition of 20-25% glycerol to the reservoir solution and then flash-cooled in liquid nitrogen.

[0268] Diffraction data were obtained at 100 K from beam lines 24-ED-C and 24-ID-E at the Advanced Photon Source using a single wavelength. Datasets from individual crystals (multiple crystals for UCAl , DH270.1 and DH270.5) were processed with HKL2000.

Molecular replacement calculations for the free Fabs were carried out with PHASER, using 13,2 from the CHI 03 lineage [Protein Data Bank (PDB) ID 4QHL] (46) or VRCOl from the VRC01/gpl20 complex [Protein Data Bank (PDB) ID 4LST] (47) as the starting models. Subsequent structure determinations were performed using DH270 lineage members as search models. The Fab models were separated into their variable and constant domains for molecular replacement.

[0269] Refinement was carried out with PHENIX, and all model modifications were carried out with Coot. During refinement, maps were generated from combinations of positional, group B-factor, and TLS (tran si ati on/1 ibrati on/screw) refinement algorithms. Secondary- structure restraints were included at ail stages for ail Fabs; noncrystallographic symmetry restraints were applied to the DH270.1 scFv and UCA3 Fab throughout refinement. The resulting electron density map for DH270.1 was further improved by solvent flattening, histogram matching, and non-cry stall ographic symmetry averaging using the program PARROT. Phase combination was disabled in these calculations. After density modification, restrained refinement was performed using Refmac in Coot. Structure validations were performed periodically during refinement using the MolProbity server. The final refinement statistics are summarized in Fig. 32.

[0270] Design of the 92BR SOSIP.664 construct

[0271] To generate the clade B FflV-l 92BR SOSIP.664 expression construct we followed established SOSIP design parameters (48). Briefly, the 92BR SOSIP.664 trimer was engineered with a disulfide linkage between gpl20 and gp41 by introducing A501C and T605C mutations (HxB2 numbering system) to covalently link the two subunits of the heterodimer (48). The I559P mutation was included in the heptad repeat region 1 (HRl) of gp41 for trimer stabilization, and part of the hydrophobic membrane proximal external region (MPER), in this case residues 664-681 of the Env ectodomain, was deleted (48). The furin cleavage site between gpl20 and gp41 (508REKR511) was altered to 506RRRRRR51 1 to enhance cleavage (48). The resulting, codon-optimized 92BR SOSIP.664 env gene was obtained from GenScript (Piscataway, NT) and cloned into pVRC-8400 as described above for Fabs using Nhel and Not!

[0272] Purification of Envs for analysis by biolayer interferometry and negative stain EM [0273] SOSIP.664 constructs were transfected along with a plasmid encoding the cellular protease furin at a 4: 1 Envlurin ratio in HEK 293F cells. Site-directed mutagenesis was performed using manufacturer's protocols (Stratagene) for mutations in the V3 region and glycosylation sites. The cells were allowed to express soluble SOSIP.664 trimers for 5-7 days. Culture superaatants were collected and cells were removed by centrifugation at 3,800 x g for 20 min, and filtered with a 0.2μηι pore size filter. SOSIP.664 proteins were purified by flowing the supernatant over a lectin (Galanthus nivalis) affinity chromatography column overnight at 4°C. The lectin column was washed with lx PBS and proteins were eluted with 0.5M methyl-a-D-mannopyranoside and 0.5M NaCl. The eluate was concentrated and loaded onto a Superdex 200 10/300 GL column (GE Life Sciences) prequilibrated in a buffer of l OmM Hepes, pH 8.0, 150mM NaCl and 0.02% sodium azide for EM, or in 2.5mM Tris, pH 7.5, 350mM NaCl, 0.02% sodium azide for binding analysis, to separate the trimer-size oligomers from aggregates and gpl40 monomers.

[0274] Electron Microscopy

[0275] Purified 92BR SOSIP.664 trimer was incubated with a five molar excess of DH270. 1 Fab at 4 °C for 1 hour. A 3μΕ aliquot containing—0,01 mg/ml of the Fab - 92BR SOSIP.664 complex was applied for 15 s onto a carbon coated 400 Cu mesh grid that had been glow- discharged at 20 mA for 30s, followed by negative staining with 2% uranyl formate for 30 s. Samples were imaged using a FEI Tecnai T12 microscope operating at 120kV, at a magnification of 52,000x that resulted in a pixel size of 2.13 A at the specimen plane. Images were acquired with a Gatan 2K CCD camera using a nominal defocus of 1,500 nm at 10° tilt increments, up to 50°. The tilts provided additional particle orientations to improve the image reconstructions,

[0276] Negative Stain Image Processing and 3D Reconstruction

[0277] Particles were picked semi-automatically using EMAN2 and put into a particle stack. Initial, reference-free, two-dimensional (2D) class averages were calculated and particles corresponding to complexes (with three Fabs bound) were selected into a sub stack for determination of an initial model. The initial model was calculated in EMAN2 using 3-fold symmetry and EMAN2 was used for subsequent refinement using 3 -fold symmetry. In total, 5,419 particles were included in the final reconstruction for the 3D average of 92BR

SOSIP.664 trimer complex with DH270.1 , The resolution of the final model was determined using a Fourier Shell Correlation (FSC) cut-off of 0.5.

[0278] Model fitting into the EM reconstructions

[0279] The cryo-EM structure of PGT128-liganded BG505 SOSIP.664 (PDB ID: 5ACO) (28) and crystal structure of DH270.1 were manually fitted into the EM density and refined by using the UCSF Chimera 'Fit in map' function.

[0280] Biolayer Interferometry

[0281] Kinetic measurements of Fab binding to Envs were carri ed out using the Octet QKe system (ForteBio); 0.2mg/mL of each His-tagged Fab was immobilized onto an anti-Human Fab-CH I biosensor until it reached saturation. The SOSIP.664 trimers were tested at concentrations of 200nM and 600nM in duplicate. A reference sample of buffer alone was used to account for any signal drift that was observed during the experiment. Association and dissociation were each monitored for 5 min. All experiments were conducted in the Octet instrument at 30°C in a buffer of 2.5mM Tris, pll 7.5, 350mM NaCl and 0.02% sodium azide with agitation at 1 ,000 rpm. Analyses were performed using nonlinear regression curve fitting using the Graphpad Prism software, version 6.

[0282] Protein Structure Analysis and Graphical Representations

[0283] The Fabs and their complexes analyzed in this study were superposed by least squares fitting in Coot. All graphical representations with protein crystal structures were made using PyMol.

[0284] Definition of immunological virus phenotypes and virus signature analysis

[0285] The maximum likelihood trees depicting the heterologous virus panel and the full set of Env sequences for the subject CH848 were created using the Los Alamos HIV database PhyML interface. HIV substitution models (49) were used and the proportion of invariable sites and the gamma parameters were estimated from the data. Illustrations were made using the Rainbow Tree interface that utilizes Ape. The analysis that coupled neutralization data with the within-subject phyiogeny based on Envs that were evaluated for neutralization sensitivity was performed using LASSIE (43). Signature analysis was performed using the methods fully described in (50, 51).

[0286] Heat maps and logo plots

[0287] Heat maps and logo plots were generated using the Los Alamos HIV database web interfaces (wvvw.hiv.lanl.gov, version Dec. 2015, HEATMAP and Analyze Align).

[0288] Selection of CH848 Env signatures for antibody lineage cooperation studies.

[0289] We previously studied cooperation between lineages that occurred soon after infection, at a time when diversity in the autologous quasispecies was limited (12), In contrast, in CH848 the earliest autologous quasispecies transition in sensitivity to

DH272/DH475 neutralization to DH270 lineage members occurred between week 39 and week 51, when multiple virus variants were circulating. Viral diversity made it impractical to test all the possible permutations or mutations from the transmitted founder virus. To select a smaller pool of candidate mutations, we sought the two most similar CH848 Env sequences at the amino acid level with opposite sensitivity to DH272/DH475 and DH270.1

neutralization around week 51 and identified clones CH0848.3.d0274.30.07 and

CH0848.3.d0358.80.06 being the most similar (sim: 0.98713), Among the differences in amino acid sequences between these two clones, the four that we selected (Δ134-143 in VI); D185N in V2; N413Y in V4; Δ463-464 in V5) were the only ones consistently different among all clones with differential sensitivity to DH272 and DH270. 1. We elected to use DH270.1 for these cooperating studies as the least mutated representative of DH270 antibodies that gained autologous neutralization at week 51. The D185N and N413Y mutations were also identified by the signature analysis shown in Fig, 19 and Fig, 36.

[0290] References and Notes

1. D, R. Burton, J. R, Mascola, Antibody responses to envelope glycoproteins in HIV-1 infection. Nature immunology 16, 571-576 (2015).

2. J. R, Mascola, B. F. Haynes, HIV-1 neutralizing antibodies: understanding nature's pathways. Immunological Reviews 254, 225-244 (2013).

3. L. M. Walker, M. Huber, K. J, Doores, E. Faikowska, R. Pejchal, J. P. Julien, S. K. Wang, A. Ramos, P. Y. Chan-Hui, M. Moyle, J. L. Mitcham, P. W. Hammond, O. A. Olsen, P. Phung, S. Fling, C, 1 1. Wong, S. Phogat, T, W^Trin, M. D. Simek, W. ( Koff, I. A. Wilson, D. R. Burton, P. Poignard, Broad neutralization coverage of HIV by multiple highly potent antibodies. Nature 477, 466-470 (2011).

4. L. M, Walker, S, K. Phogat, P. Y. Chan-Hui, D. Wagner, P. Phung, J. L. Goss, T. Wrin, M. D. Simek, S. Fling, J. L. Mitcham, J. K. Lehrman, F. H. Priddy, O. A. Olsen, S. M. Frey, P. W. Hammond, S. Kaminsky, T. Zamb, M. Moyle, W, C. Koff P. Poignard, D, R. Burton, Broad and potent neutralizing antibodies from an African donor reveal a new HIV-1 vaccine target. Science 326, 285-289 (2009),

5. K. J. Doores, L. Kong, S. A. Krumm, K. M. Le, D. Sok, U. Laserson, F. Garces, P. Poignard, I. A. Wilson, D. R. Burton, Two classes of broadly neutralizing antibodies within a single lineage directed to the high-mannose patch of HIV envelope. Journal of virology 89, 1105-1 1 18 (2015),

6. D, Sok, K. J, Doores, B. Briney, K. M. Le, K. L. Saye-Francisco, A. Ramos, D. W. Kulp, J. P. Julien, S. Menis, L. Wickramasinghe, M. S. Seaman, W. R. S chief, I. A. Wilson, P. Poignard, D, R. Burton, Promiscuous glycan site recognition by antibodies to the high- mannose patch of gpl20 broadens neutralization of HIV. Science translational medicine 6, 236ra263 (2014).

7. D. Sok, U. Laserson, J. Laserson, Y. Liu, F. Vigneault, J. P. Julien, B. Briney, A. Ramos, K. F. Saye, K. Le, A. Mahan, S. Wang, M. Kardar, G. Yaari, L. M. Walker, B. B. Simen, E. P. St John, P. Y. Chan-Hui, K. Swiderek, S. H. Kleinstein, G. Alter, M. S. Seaman, A. K. Chakraborty, D. K oiler, I. A. Wilson, G. M. Church, D. R. Burton, P. Poignard, The effects of somatic hypermutation on neutralization and binding in the PGT121 family of broadly neutralizing HIV antibodies. PLoS pathogens 9, e!003754 (2013). 8. H. Mouquet, L. Scharf, Z. Euler, Y. Liu, C. Eden, J. F. Scheid, A. Halper-Stromberg, P. N. Gnanapragasam, D. I. Spencer, M. S. Seaman, H. Schuitemaker, T, Feizi, M. C.

Nussenzweig, P. J. Bjorkman, Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies. Proceedings of the National Academy of Sciences of the United States of America 109, E3268-3277 (2012),

9. B. F. Haynes, G. Kelsoe, S. C. Harrison, T. B. Kepler, B-cell-!ineage immunogen design in vaccine development with HIV-1 as a case study. Nature Biotechnology 30, 423- 433 (2012).

10. H. X. Liao, R. Lynch, T. Zhou, F. Gao, S. M. Alam, S. D. Boyd, A. Z. Fire, K. M. Roskin, C. A. Schramm, Z. Zhang, J. Zhu, L. Shapiro, J. C. Mullikin, S. Gnanakaran, P. Hraber, K. Wiehe, G. Kelsoe, G. Yang, S. M. Xia, D. C. Montefiori, R. Parks, K. E. Lloyd, R. M. Scearce, K. A. Soderberg, M. Cohen, G. Kamanga, M. K. Louder, L. M. Tran, Y. Chen, F. Cai, S. Chen, S. Moquin, X. Du, M. G. Joyce, S. Srivatsan, B. Zhang, A. Zheng, G. M. Shaw, B. H. Hahn, T. B. Kepler, B. T. Korber, P. D. Kwong, J. R. Mascola, B. F. Haynes, Co-evolution of a broadly neutralizing HIV-1 antibody and founder vims. Nature 496, 469- 476 (2013),

1. M. Bonsignori, T. Zhou, Z. Sheng, L. Chen, F. Gao, M. G. Joyce, G. Ozorowski, G. Y. Chuang, C. A. Schramm, K. Wiehe, S. M. Alam, T. Bradley, M. A. Gladden, K. K.

Hwang, S. Iyengar, A. Kumar, X. Lu, K. Luo, M. C. Mangiapani, R. J. Parks, H. Song, P. Acharya, R. T. Bailer, A. Cao, A. Druz, I. S. Georgiev, Y. D. Kwon, M. K. Louder, B.

Zhang, A. Zheng, B. J. Hill, R. Kong, C. Soto, J. C. Mullikin, D. C. Douek, D. C. Montefiori, M. A. Moody, G M. Shaw, B. H. Hahn, G. Kelsoe, P. T. Hraber, B. T. Korber, S. D. Boyd, A. Z. Fire, T. B. Kepler, L. Shapiro, A. B. Ward, J. R. Mascola, H. X. Liao, P. D. Kwong, B. F. Haynes, Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody, Cell 165, 449-463 (2016).

12. F. Gao, M. Bonsignori, H. X. Liao, A. Kumar, S. M. Xia, X. Lu, F. Cai, K. K. Hwang, H. Song, T. Zhou, R. M. Lynch, S. M. Alam, M. A. Moody, G, Ferrari, M. Berrong, G, Kelsoe, G. M. Shaw, B. H. Hahn, D. C. Montefiori, G. Kamanga, M. S. Cohen, P. Hraber, P. D. Kwong, B. T. Korber, J. R. Mascola, T. B. Kepler, B. F. Haynes, Cooperation of B cell lineages in induction of HIV-1 -broadly neutralizing antibodies. Cell 158, 481 -491 (2014).

13. M. Pancera, T. Zhou, A. Druz, I. S. Georgiev, C. Soto, J. Gorman, J. Huang, P.

Acharya, G. Y. Chuang, G. Ofek, G. B. Stewart- Jones, J. Stuckey, R. T. Bailer, M. G. Joyce, M. K. Louder, N. Tumba, Y. Yang, B. Zhang, M. S. Cohen, B. F. Haynes, J. R. Mascola, L. Morris, J. B. Munro, S. C. Blanchard, W. Mothes, M. Connors, P. D. Kwong, Structure and immune recognition of trimeric pre-fusion HIV-l Env. Nature 514, 455-461 (2014).

14. M. Bonsignori, K, K. Hwang, X. Chen, C. Y. Tsao, L, Morris, E. Gray, D. J.

Marshall, J. A. Crump, S. H. Kapiga, N. E. Sam, F. Sinangil, M. Pancera, Y. Yongping, B. Zhang, J. Zhu, P. D. Kwong, S. O'Dell, J, R. Mascola, L. Wu, G. J. Nab el, S. Phogat, M. S. Seaman, J. F. Whitesides, M. A. Moody, G. Kelsoe, X. Yang, J. Sodroski, G. M. Shaw, D. C. Montefiori, T. B. Kepler, G. D. Tomaras, S. M. Alam, H. X. Liao, B. F. Haynes, Analysis of a clonal lineage of HIV-l envelope V2/V3 conformational epitope-specific broadly neutralizing antibodies and their inferred unmutated common ancestors. Journal of virology 85, 9998-10009 (201 1).

15. E. S. Gray, M. A. Moody, C. K. Wibmer, X. Chen, D. Marshall, J. Amos, P. L, Moore, A. Foulger, J. S. Yu, B. Lambson, S. Abdool Karim, J. Whitesides, G. D. Tomaras, B. F. Haynes, L. Morris, H. X. Liao, Isolation of a monoclonal antibody that targets the alpha-2 helix of gpl 20 and represents the initial autologous neutralizing-antibody response in an HIV-l subtype C-infected individual. Journal of virology 85, 7719-7729 (201 1).

16. S. M. Alam, B. Aussedat, Y. Vohra, R. R. Meyerhoff, E. M. Cale, W. E. Walkowicz, N. A. Radakovich, L. Armand, R. Parks, L. Sutherland, R. Scearce, M. G. Joyce, M. Pancera, A. Druz, I. Georgiev, T. Von Holle, A. Eaton, C. Fox, S. G. Reed, M. K. Louder, R. T.

Bailer, L. Morris, S. Abdool Karim, M. Cohen, H. X. Liao, D. Montefiori, P. K. Park, A. Fernandez-Tejada, K. Wiehe, S. Santra, T. B. Kepler, K. O. Saunders, J. Sodroski, P. D, Kwong, J. R. Mascola, M. Bonsignori, M. A. Moody, S. J. Danishefsky, B. F. Haynes, Mimicry of an HIV broadly neutralizing antibody epitope with a synthetic glycopeptide, under review.

17. R. Pejchal, K. J. Doores, L. M. Walker, R. Khayat, P. S. Huang, S. K. Wang, R. L. Stanfield, J. P, ien, A. Ramos, M. Crispin, R. Depetris, U. Katpally, A. Marozsan, A. Cupo, S. Maloveste, Y. Liu, R. McBride, Y. Ito, R. W. Sanders, C. Ogohara, J. C. Paulson, T. Feizi, C. N. Scanlan, C. H. Wong, J. P. Moore, W. C. Olson, A. B. Ward, P. Poignard, W. R. Schief, D. R. Burton, I. A. Wilson, A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. Science 334, 1097-1103 (201 1).

18. B. Aussedat, Y. Vohra, P. K. Park, A. Fernandez-Tejada, S. M. Alam, S. M.

Dennison, F. H. Jaeger, K. Anasti, S. Stewart, J. H. Blinn, H. X. Liao, J. G. Sodroski, B. F. Haynes, S. J. Danishefsky, Chemical synthesis of highly congested gpl20 V1V2 N- glycopeptide antigens for potential HIV- 1 -directed vaccines. Journal of the America

Chemical Society 135, 131 13-13120 (2013). 19. S. M. Aiam, S. M. Dennison, B. Aussedat, Y. Vohra, P. . Park, A. Fernandez-

Tejada, S. Stewart, F. H. Jaeger, K. Anasti, J. H. Blinn, T. B. Kepler, M. Bonsignori, H. X. Liao, J. G. Sodroski, S. J. Danishefsky, B, F. Haynes, Recognition of synthetic glycopeptides by HIV-1 broadly neutralizing antibodies and their unmutated ancestors. Proc Natl Acad Sci USA 110, 18214-18219 (2013),

20. G. Yaari, J. A. Vander Heiden, M. Uduman, D. Gadala-Maria, N. Gupta, J. N. Stern, K. C, O'Connor, D. A. Hafler, U. Laserson, F. Vigneault, S. H. Kleinstein, Models of somatic hypermutation targeting and substitution based on synonymous mutations from high- throughput immunoglobulin sequencing data. Frontiers in immunology 4, 358 (2013).

21. We accessed the SF5 mutability model dataset at

http : // clip . m ed ,y al e , edu/shm/ downl oad . php .

22. L. Kong, J. H, Lee, K. J. Doores, C. D. Murin, J, P. Julien, R, McBride, Y, Liu, A. Marozsan, A. Cupo, P. J. Klasse, S. Hoffenberg, M. Caulfieid, C. R. King, Y. Hua, K. M. Le, R, Khayat, M. C, Deller, T. Clayton, H. Tien, T. Feizi, R. W. Sanders, J. C. Paulson, J, P. Moore, R. L. Stanfield, D. R. Burton, A. B. Ward, I. A. Wilson, Supersite of immune vulnerability on the glycosylated face of HIV-1 envelope glycoprotein gpl20. Nature structural & molecular biology! 20, 796-803 (2013).

23. J. P. Julien, D. Sok, R. Khayat, J. H. Lee, K. J. Doores, L, M. Walker, A. Ramos, D. C. Diwanji, R. Pejchal, A. Cupo, U. Katpally, R. S. Depetris, R. L. Stanfield, R. McBride, A. J. Marozsan, J. C. Paulson, R. W. Sanders, J. P. Moore, D. R. Burton, P. Poignard, A. B. Ward, I. A. Wilson, Broadly neutralizing antibody PGT121 allosterically modulates CD4 binding via recognition of the HIV-1 g l20 V3 base and multiple surrounding glycans. PLoS pathogens 9, el 003342 (2013).

24. M. Pancera, Y. Yang, M. K. Louder, J. Gorman, G. Lu, J. S. McLeilan, J. Stuckey, J. Zhu, D. R. Burton, W. C, Koff, J. R. Mascola, P. D. Kwong, N332-Directed broadly neutralizing antibodies use diverse modes of HIV-1 recognition: inferences from heavy-light chain complementation of function. PloS one 8, e55701 (2013).

25. P. L. Moore, E. S. Gray, C. K. Wibmer, J. N. Bhiman, M. Nonyane, D. J. Sheward, T. Hermanus, S. Bajimaya, N. L. Tumba, M. R. Abrahams, B. E, Lambson, N. Ranchobe, L. Ping, N. Ngandu, Q. Abdool Karim, S. S. Abdool Karim, R. I. Swanstrom, M. S. Seaman, C. Williamson, L. Morris, Evolution of an HIV glycan-dependent broadly neutralizing antibody epitope through immune escape. Nature medicine 18, 1688-1692 (2012).

26. LANL HIV Sequence Database

(http://www.hiv.lanl .gov/content/sequence/HIV/mainpage.html) 27. F. Garces, D. Sok, L. Kong, R. McBride, H. J. Kim, K. F. Saye-Francisco, J. P. ien, Y. Hua, A, Cupo, J. P. Moore, J, C. Paulson, A, B. Ward, D. R. Burton, I. A. Wilson, Structural evolution of glycan recognition by a family of potent HIV antibodies. Cell 159, 69- 79 (2014).

28. J, H. Lee, N. de Val, D Lyumkis, A, B. Ward, Model Building and Refinement of a Natively Glycosylated HIV-1 Env Protein by High-Resolution Cryoelectron Microscopy. Structure 23, 1943-1951 (2015).

29. F. Garces, J. H. Lee, N. de Val, A. T. de la Pena, L. Kong, C. Puchades, Y. Hua, R. L. Stanfield, D. R. Burton, J. P. Moore, R, W, Sanders, A. B. Ward, I. A. Wilson, Affinity Maturation of a Potent Family of HIV Antibodies Is Primarily Focused on Accommodating or Avoiding Glycans. Immunity 43 , 1053-1063 (2015).

30. M. Bonsignori, D. C. Montefiori, X. Wu, X, Chen, K, K. Hwang, C, Y. Tsao, D. M. Kozink, R. J. Parks, G. D. Tomaras, J. A. Crump, S. H. Kapiga, N. E. Sam, P. D. Kwong, T.

B, Kepler, H. X. Liao, J. R. Mascola, B. F. Haynes, Two distinct broadly neutralizing antibody specificities of different clonal lineages in a single HIV-1 -infected donor:

implications for vaccine design. Journal of virology 86, 4688-4692 (2012).

31. K. Wagh, T. Bhattacharya, C. Williamson, A. Robies, M. Bayne, J. Garrity, M. Rist,

C. Rademeyer, H. Yoon, A. Lapedes, H. Gao, K. Greene, M. K. Louder, R. Kong, S. A.

Karim, D. R. Burton, D. H. Barouch, M. C. Nussenzweig, J. R. Mascola, L. Morris, D. C. Montefi ori, B. Korber, M. S. Seaman, Optimal Combinations of Broadly Neutrali zing Antibodies for Prevention and Treatment of HIV-1 Ciade C Infection. PLoS pathogens 12, el005520 (2016).

32. L. S, Yeap, J. K. Hwang, Z. Du, R. M. Meyers, F. L. Meng, A, Jakubauskaite, M. Liu, V. Mani, D. Neuberg, T. B. Kepler, J. H. Wang, F. W. Alt, Sequence-Intrinsic Mechanisms that Target AID Mutational Outcomes on Antibody Genes. Cell 163, 1124-1 137 (2015),

33. G. D. Tomaras, N. L. Yates, P. Liu, L. Qin, G. G. Fouda, L. L. Chavez, A. C.

Decamp, R. J. Parks, V. C. Ashley, J. T. Lucas, M. Cohen, J. Eron, C, B. Hicks, H. X. Liao, S. G, Self, G. Landucci, D. N. Forthai, K. J. Weinhold, B. F. Keele, B. H. Hahn, M. L.

Greenberg, L. Morris, S. S, Karim, W, A. Blattner, D. C. Montefiori, G. M. Shaw, A, S. Perelson, B. F. Haynes, Initial B-celi responses to transmitted human immunodeficiency vims type 1 : virion-binding immunoglobulin M (IgM) a d IgG antibodies followed by plasma anti-gp41 antibodies with ineffective control of initial viremia. Journal of virology 82, 12449-12463 (2008). 34. G. M. Shaw, E. Hunter, HIV transmission. Cold Spring Harbor perspectives in medicine 2, (2012),

35. W. B. Williams, H. X. Liao, M. A. Moody, T. B. Kepler, S. M. Alam, F. Gao, . Wiehe, A. M. Trama, K. Jones, R. Zhang, H. Song, D. J. Marshall, J. F. Whitesides, K.

Sawatzki, A. Hua, P. Liu, M. Z. Tay, K. E. Seaton, X, Shen, A. Foulger, K. E. Lloyd, R. Parks, J. Pollara, G. Ferrari, J. S. Yu, N. Vandergrift, D. C. Montefiori, M. E. Sobieszczyk, S. Hammer, S. Karuna, P. Gilbert, D. Grove, N. Grunenberg, M. J, McElrath, J. R. Mascola, R, A. Koup, L. Corey, G. J. Nabel, C. Morgan, G. Churchyard, J. Maenza, M. Keefer, B. S. Graham, L. R. Baden, G. D. Tomaras, B. F. Haynes, HIV-1 VACCINES. Diversion of HIV- 1 vaccine-induced immunity by gp41-microbiota cross-reactive antibodies. Science 349, aabl253 (2015).

36. T. B. Kepler, Reconstructing a B-cell clonal lineage. I. Statistical inference of unobserved ancestors. F1000R.es 2, 103 (2013).

37. L. G Cowell, T. B. Kepler, The nucleoti de-replacement spectrum under somatic hypermutation exhibits microsequence dependence that is strand-symmetric and distinct from that under germline mutation. Journal of immunology 164, 1971-1976 (2000),

38. A. G. Betz, C. Rada, R. Pannell, C. Milstein, M. S. Neuberger, Passenger transgenes reveal intrinsic specificity of the antibody hypermutation mechanism: clustering, polarity, and specific hot spots. Proceedings of the National Academy of Sciences of the United States of America 90, 2385-2388 (1993).

39. R. Bransteitter, P. Pham, P. Calabrese, M. F. Goodman, Biochemical analysis of hypermutational targeting by wild type and mutant activation-induced cytidine deaminase. The Journal of biological chemistry 279, 51612-51621 (2004),

40. M. S. Seaman, H. Janes, N. Hawkins, L. E. Grandpre, C. Devoy, A. Giri, R. T.

Coffey, L. Harris, B. Wood, M. G. Daniels, T. Bhattacharya, A. Lapedes, V. R. Polonis, F. E. McCutchan, P. B. Gilbert, S. G. Self, B. T. Korber, D. C. Montefiori, J. R. Mascola, Tiered categorization of a diverse panel of HIV-1 Env pseudoviruses for assessment of neutralizing antibodies. Journal of virology 84, 1439-1452 (2010).

41. J. F, Sal azar-Gonzalez, M. G. Salazar, B. F, Keele, G. H. Learn, E. E, Giorgi, H. Li, J. M. Decker, S. Wang, J. Baalwa, M. H. Kraus, N. F. Parrish, K. S. Shaw, M. B. Guffey, K. J. Bar, K. L. Davis, C. Ochsenbauer-Jambor, J. C. Kappes, M. S. Saag, M. S, Cohen, J, Mulenga, C. A. Derdeyn, S. Allen, E. Hunter, M. Markowitz, P. Hraber, A. S. Pereison, T. Bhattacharya, B. F. Haynes, B. T. Korber, B. H. Hahn, G. M. Shaw, Genetic identity, biological phenotype, and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infection. The Journal of experimental medicine 206, 1273-1289 (2009),

42. B. F. Keele, E. E. Giorgi, J. F. Salazar-Gonzalez, J. M. Decker, K, T, Pham, M, G. Salazar, C. Sun, T. Grayson, S. Wang, H. Li, X. Wei, C. Jiang, J. L. Kirchherr, F. Gao, J. A. Anderson, L, H. Ping, R. Swanstrom, G. D, Tomaras, W. A. Blattner, P. A. Goepfert, J, M. Kilby, M. S. Saag, E. L. Delwart, M. P. Busch, M. S. Cohen, D. C. Montefiori, B. F. Haynes, B. Gaschen, G. S. Athreya, H. Y. Lee, N. Wood, C. Seoighe, A. S, Perelson, T. Bhattacharya, B. T. Korber, B. H. Hahn, G. M. Shaw, Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proceedings of She National Academy of Sciences of the United States of America 105, 7552-7557 (2008).

43. P. Hraber, B. Korber, K. Wagh, E. E. Giorgi, T. Bhattacharya, S. Gnanakaran, A. S. Lapedes, G. H. Learn, E, F. Kreider, Y. Li, G M. Shaw, B. H. Hahn, D. C, Montefiori, S. M. Aiam, M. Bonsignori, M. A. Moody, H. X. Liao, F. Gao, B. F. Haynes, Longitudinal

Antigenic Sequences and Sites from Intra-Host Evolution (LASSIE) Identifies Immune- Selected HIV Variants. Viruses 7, 5443-5475 (2015).

44. J. L. Kirchherr, X. Lu, W. Kasongo, V. Chalwe, L. Mwananyanda, R M. Musonda, S. M. Xia, R. M. Scearce, H. X. Liao, D. C. Montefiori, B. F. Haynes, F. Gao, High throughput functional analysis of HIV-1 env genes without cloning. Journal of virological methods 143, 104-11 1 (2007).

45. E. P. Go, A. Herschhorn, C. Gu, L. Castillo-Menendez, S. Zhang, Y. Mao, H. Chen, H. Ding, J. K. Wakefield, D. Hua, H. X. Liao, J. C. Kappes, J. Sodroski, H. Desaire,

Comparative Analysis of the Glycosylation Profiles of Membrane- Anchored HIV-1 Envelope Glycoprotein Trimers and Soluble gpl40. Journal of virology 89, 8245-8257 (2015),

46. D. Fera, A. G. Schmidt, B. F. Haynes, F. Gao, H. X. Liao, T. B. Kepler, S. C.

Harrison, Affinity maturation in an HIV broadly neutralizing B-cell lineage through reorientation of variable domains. Proceedings of the National Academy of Sciences of the United Stales of America I I I , 10275-10280 (2014),

47. T. Zhou, J. Zhu, X. Wu, S. Moquin, B. Zhang, P. Aeharya, I. S. Georgiev, H. R.

Altae-Tran, G. Y, Chuang, M. G. Joyce, Y. D. Kwon, N. S. Longo, M. K. Louder, T, Luongo, K. McKee, C. A. Schramm, J. Skinner, Y. Yang, Z. Yang, Z. Zhang, A. Zheng, M.

Bonsignori, B. F. Haynes, J, F. Scheid, M. C. Nussenzweig, M. Simek, D. R. Burton, W. C. Koff, J. C. Mullikin, M. Connors, L. Shapiro, G. J. Nabel, J. R. Mascola, P. D. Kwong, Multidonor analysis reveals structural elements, genetic determinants, and maturation pathway for HIV-1 neutralization by VRCOl -class antibodies. Immunity 39, 245-258 (2013). 48. R. W. Sanders, R. Derking, A. Cupo, J. P. ien, A. Yasmeen, N. de Val, H. J. Kim, C. Blattner, A. T. de la Pena, J. Korzun, M. Golabek, K. de Los Reyes, T. J. Ketas, M. J. van Gils, C. R. King, I, A. Wilson, A. B. Ward, P. J. Klasse, J. P. Moore, A next-generation cleaved, soluble HIV- 1 Env trimer, BG505 SOSIP.664 gp^'I40, expresses multiple epitopes for broadly neutralizing but not non-neutralizing antibodies. PLoS pathogens 9, el 003618 (2013).

49. D. C. ickle, L. Heath, M. A. Jensen, P. B. Gilbert, J. I. Mullins, S. L. Kosakovsky Pond, HIV-specific probabilistic models of protein evolution. PloS one 2, e503 (2007).

50. S. Gnanakaran, M. G. Daniels, T. Bhattacharya, A. S. Lapedes, A. Sethi, M. Li, H. Tang, K. Greene, H. Gao, B. F. Haynes, M. S. Cohen, G. M. Shaw, M. S. Seaman, A. Kumar, F. Gao, D. C. Montefiori, B. Korber, Genetic signatures in the envelope glycoproteins of HIV-1 that associate with broadly neutralizing anti bodies. PLoS computational biology 6, el000955 (2010).

51. T. Bhattacharya, M, Daniels, D. Heckerman, B, Foley, N. Frahm, C. Kadie, J.

Carlson, K. Yusim, B. McMahon, B. Gaschen, S. Maliai, J. I. Mullins, D. C. Nickle, J.

Herbeck, C. Rousseau, G. H, Learn, T. Miura, C. Brander, B. Walker, B. Korber, Founder effects in the assessment of HIV polymorphisms and HLA allele associations. Science 315, 1583-1586 (2007),

52. L. Kong, A. Torrents de la Pena, M. C. Deller, F. Garces, K. Sliepen, Y. Hua, R. L. Stanfield, R. W. Sanders, I. A. Wilson, Complete epitopes for vaccine design derived from a crystal structure of the broadly neutralizing antibodies PGT128 and 8ANC195 in complex with an HIV-1 Env trimer. Acta crystallographies Section D, Biological crystallography 71, 2099-2108 (2015).

[0291] Data and materials availability.

[0292] The V(D)J rearrangement sequences of DH272, DH475 and the DH270 lineage antibodies (DH270.UCA, DH270.IA1 through IA4, and DH270.1 through 6) have been deposited in GenBank with accession numbers KY354938 through KY354963. NGS sequence data for clones DH270, DH272 and DH475 have been deposited in GenBank with accession numbers KY347498 through KY347701. Coordinates and structure factors for UCAl , IJCA3, DH270.1, DH270.3, DH270.5, DH270.6, and DH272 have been deposited in the Protein Data Bank with accession code 5U0R, 5U15, 5U0U, 5TPL, 5TPP, 5TQA, and 5TRP, respectively. The EM map of the 92BR SOSIP.664 trimer in complex with DH270.1 has been deposited in the EM Data Bank with accession code EMD-8507.

Example 2 Man9-V3 glycopeptides and agiycone peptides [0293] This example provides non-limiting embodiments of V3 peptides which can be used in the immunogenic compositions and methods.

[0294] The crystal structure of the HIV-1 V3 bnAb PGT128 in complex with gpl 20 outer domain containing a truncated V3 loop revealed the key antibody contacts with its glycosylated epitope (R. Pejchal et al, Science (New York, N.Y.) 334, 1097 (201 1 )).. We constructed a glycosylated peptide (Maii9-V3) that is comprised of the discontinuous epitope of PGT128 with deletion of residues 305-320, retention of P³²ⁱ, and stabilization by a disulfide bridge between C²⁹⁶ and C ^{J 1} (Figure 38A-E) (R. Pejchal et al., Science (New York, N.Y.) 334, 1097 (201 1)). Mari9-V3 glycopeptide was synthesized using a similar synthetic approach used to produce VI V2 giycopeptides (B. Aussedat et al., J Am ( ^'hens Soc 135, 131 3 (2013)). As controls, a biotinylated aglycone-V3 peptide with no high mannose glycans (Fig, 38C) and a biotinylated Mam free glycan (Fig. 38A) were also synthesized.

[0295] V3-glycan bnAb DH270 unmutated common ancestor binding to the peptide component of Man<>-V3 glycopeptide

[0296] The unmutated common ancestor (UCA) and the earlier intermediates in the DH270 lineage showed no detectable binding to either soluble or to cell surface Env. However, the DH270 bnAb UCA did bind to Man<>V3 (Figure 27A) and, as well, bound to the aglycone- V3 (Figures 27B). Similarly, the early intermediate antibodies (IA4, IA3, IA2) each bound to both Man9V3 and aglycone-V3, and their binding was stronger to the aglycone- V3 compared to the Mari9-V3glycopeptide (Figures 27B). Binding to the Man9-V3 glycopeptide remained low (>10μΜ) up to the DH270.1 bnAb lineage member (Figures 27A), when the affinity increased (coincident with nucleotide mutations up to a frequency of 5.6%) to a Kd of 33 I nM with better binding to the glycopeptide than to aglycone-V3 (Figures 27A-B). Thereafter in the DH270 bnAb lineage as mutations accumulated, binding to the Man9-V3 glycopeptide increased, culminating in a Kd of 188 nM in the most potent bnAb, DH270.6, and no binding to aglycone- V3 peptide (Figures 27A-B). Thus, both the Man9-V3 glycopeptide and the aglycone-V3 peptide bound the DH270.UCA with the lineage member binding independent of glycans until the DH270 lineage acquired a frequency of ~6% nucleotide mutations.

[0297] Additional V3 peptides contemplated by the invention are listed in Table 2.

Table 2 below includes non-limiting examples of V3 peptides

CH848.TF V3 293-32 -biotin EI VCTRPGNNTRK S VR1 GPGQTF Y ATGK

CH848.TF V3 297-324-biotin TRPGNNTRKSVRIGPGQTFYATGDIIGK CH848. TF_V3_303 -330-biotin TRKSVRIGPGQTFYATGDIIGDIRQAHK CH848. TF_V 3 307-334-biotin VRIGPGQTFYATGDIIGDIRQAHCNISK CH848.TF_V3_315-340-biotin QTFYATGDIIGD1RQAHCNISERQWNK CH848.TF V3 biotin-315-340 KQTFYATGDIIGDIRQAHCNISERQWNK CH848.0949, 10. 17_V3_293-321-biotin EIVCTRPNNNTRKSVRIGPGQTFYATGK CH848.0949.10.17 _V3 297-324-biotin TRP NTRKSVRIGPGQTFYATGDIIGK CH 848.0949.10.17_ V3_303 -33 O-bioti n TRKSVRIGPGQTFYATGDIIGDIKQAHK CH848.0949.10.17_V3_307-334-biotin VRIGPGQTFYATGDIIGDI QAHCNISK CH848.0949, 10. 17 V3 315-340-biotin QTFYATGDIIGDIKQAHCNISEEKWNDK CH848.0949.10.17 V3 biotin-315-340 KQTF YATGDIIGDIKQAHCNI SEEKWND

[0298] Peptide sequences above in order of appearance in Table 2 are SEQ ID Nos: 2 to 13. Peptides sequence from Table 2 without N-or C -terminal biotinylation lysine are SEQ ID NOs: 14 to 25.

[0299] Any of the peptides could be biotinvlated. In some embodiments, the peptides are biotinylated on the C terminus, except CH848.TF V3 biotin-315-340 and

CH848.0949.10.17_V3_biotin-315-340, which are biotinvlated on the N terminus.

[0300] The peptides of the invention can be synthesized by any known method. V3 aglycone of and Man9V3 and their synthesis are provided in Figure 38A, See also WO2014/7172366, [0301] In some embodiments, the peptides are:

CH848.TF_V3_293-321-biotin EIVCTRPG NTRKSVRIGPGQTFYATGK-Biotin

CH848.TF_V3_297-324-biotin TRPGNNTRKSVRIGPGQTFYATGDIIGK-Biotin

CH848. TF_V3_303 -330-bi otin TRK S VRIGPGQTF YATGDIIGDIRQ AH -Biotin

CH848. TF_V3_307-334-biotin VRIGPGQTFYATGDIIGDIRQAHCNISK-Bi otin

CH848.TF_ V3 _315-340-biotin QTFYATGDIIGDIRQAHC ISERQWNKK-Biotin

[0302] It is readily understood that peptides which are not biotinylated do not include an N- or C-terminal lysine (or other specific functional groups or residues) for targeting with biotynaltion reagents. )3] V3 (+ the base containing N332 NGS) of CH848 transmitted founder and CH0848.0949.10.17 are shown below:

TF EIVCTRPGNNTRKSVMGPGQTFYATGDnGDIRQAHC SERQW K

(SEQ ID NO: 26 with lysine and SEQ ID NO: 27 without terminal lysine).

CH0848.0949.10.17 EIVCTRPNNNTRKSVRIGPGQTFYATGDIIGDIKQAHCNISEE

KWND (SEQ ID NO: 28).

[0304] Non-limiting embodiments of V3 peptides variants include:

[0305] a. Wildtype EINC TRPNNNTRPGEIIGDIRQ AHCNISRA (SEQ ID NO: 29 )

[0306] b. GAIA EINCTRPNNNTRPGEIIGAIAQAHCNISRA (SEQ ID NO: 30 ) [0307] c. GDIA ISRA (SEQ ID NO: 31 )

[0308] d. GAIR: EINCTRPNNNTRPGEIIGAIRQ AHCNISRA (SEQ ID NO: 32 ) [0309] e. ADAR: EINCTRPNNNTRPGEIIA JARQ AHCNISRA (SEQ ID NO: 33 ) [0310] The peptides of the invention could be glycosylated at either or both positions N301 and N332. In some embodiments the giycan is Man9GicNAc2.

[0311] It is readily understood that peptides which are not biotinylated do not include an N- or C-terminal lysine (or other specific functional groups or residues) for targeting with biotynaltion reagents.

[0312] The invention also contemplates peptides which comprise T-cell helper epitope. One non-limiting embodiment includes GTH1 helper epitope. The helper epitope(s) could be at the N-or C -terminus of the peptide.

[0313] The peptides of the invention could be multimerized. In some embodiments, the peptides are biotinylated or multimerized. In some embodiments, the multimeric peptides comprise a T-helper epitope, e.g. but not limited to GTHl epitope. The helper epitope(s) could be at the N-or C-terminus of the peptide. In some embodiments, the peptides are conjugated to a lipid and then multimerized. The lipids could be pegylated. A non-limiting example is V3 (SEQ ID NO: 1) Peg-GTHl -DPPE peptide.

Example 3 : Selections of immunogens to induce and boost V3 antibodies

[0314] The following example provides non-limiting embodiments of immunogens and combination of immunogens for use in various immunization schedules.

[0315] All selections need a prime which engages the UCA. Non-limiting examples are Some of the general considerations in choosing immunogens for boost in induction of V3 giycan antibodies are as follows: (i) ~ activate IA4, select for rare mutation; (ii)— select for antibodies that favor the trimer, expand the variation in the autologous signature residue to potentially expand recognition of diversity in the population; (iii) -expose the maturing antibodies to longer loops, even though these viruses are not bound or neutralized as well as viruses with shooter loops, as this is the main constrain on heterologous population breadth and that is what is needed. In some embodiments, immunogens are selected which can do (i) and (iii). In other embodiments, the selection includes immunogens which can do (ii).

[0316] Any suitable form of the envelope could be used for prime and/or boost. The envelope used in the compositions and methods of the invention can be a gpl60, gp l 50, gpl45, gpl40, gpl20, gp41 , N-terminal deletion variants as described herein, cleavage resistant variants as described herein, or codon optimized sequences thereof. In certain embodiments the composition comprises envelopes as ί rimers. In certain embodiments, envelope proteins are mutimerized, for example trimers are attached to a particle such that multiple copies of the trimer are attached and the muitimerized envelope is prepared and formulated for immunization in a human. In certain embodiments, the compositions comprise envelopes, including but not limited to trimers as particulate, high-density array on liposomes or other particles, for example but not limited to nanoparticles. In some embodiments, the trimers are in a well ordered, near native like or closed conformation. In some embodiments the trimer compositions comprise a homogenous mix of native like trimers.

[0317] Near-native soluble trimers using the 6R.SOSIP.664 design are capable of generating autologous tier 2 neutralizing plasma antibodies in the plasma (Sanders et al. 2015), which provides a starting point for designing immunogens to elicit broadly neutralizing antibodies. While these trimers are preferentially antigenic for neutralizing antibodies they still possess the ability to expose the V3 loop, which generally results in strain-specific binding and neutralizing antibodies after vaccination. Using the unliganded structure the

BG505.6R.SOSIP.664 has been stabilized by adding cysteines at position 201 and 433 to constrain the conformational flexibility such that the V3 loop is maintained unexposed (Kwon et al. 2015).

[0318] Stabilized Trimer Immunogen Design. Several SOSIP trimer designs have been generated: 6R.SOSiP.664, disulfide stabilized (DS) 6R.SOSIP.664 (Kwon et al Nature Struc Mol Biol 2015), 6R,SOSIP.664v4.1 (DeTaeye et al. Ceil 2016), and 6R.SOSIP.664v4.2 (DeTaeye et al. Cell 2016). The CH848 SOSIP is made as a chimera of C.CH848 and A.BG505. Sequences of various CH848 envelope trimer designs are illustrated in Figures 39A-B, 40A-C, and 41A-C. Any one of the CH848 envelope sequences from

WO2015/153638 could be designed as SQSIP trimers,

[0319] In some embodiments the CHIM.6R.SOSIP.664V4.1 and/or

CHIM.6R.SOSIP.664V4. Idesign is expected to be in closed stabilized conformation. This design is expected to show preferential binding to broad neutralizing anti bodies compared to binding to non-neutralizing antibodies. This design is expected to bind to antibodies from the V3 antibodies of Example 1.

[0320] The gpl20 of C.CH848 envelope was fused with the BG505 inner domain gp 120 sequence within the alpha helix 5 (alphaS) to result in a chimeric protein. The chimeric gpl20 is disulfide linked to the A.BG505 gp41 as outlined by Sanders et al. (PLOS Path 2013). In some embodiments the immunogens were designed as chimeric proteins that possess the BG505 gp41 connected to the CH848 gpl20, since the BG505 strain is particularly adept at forming well-folded, closed state trimers (See Figure 41C). This envelope design retains the CH848 base of the V3 loop and glycan(s) that are targeted by the DH270 lineage of broadly neutralizing antibody lineages that were isolated from CH848 (Example 1).

[0321] Provided are non-limiting examples of selections of envelopes for immunization to induce neutralizing FflV-1 antibodies, including but not limited to broadly neutralizing antibodies with the specificity of antibodies from the DH270 lineage. One non-limiting embodiment of the V3 peptide used as a prime is:

1 · i \^'( TR N \ N TRI'Gi; I IGDI RQAi M S A (SEQ ID NO: 1) as aglycone or as

Man9GlcNAc2 glycosylated at both N301 and N332.

[0322] The envelopes could be administered in any suitable form, as nucleic acids, amino acids and/or combination, a gpl60, gpl50, gpl45, any suitable form of a trimer, for example but not limited to SOSIP trimers, preferably in a closed conformation , gpl 40 (including but not limited to gpl40C, gpl40CF, gpl40CFI), gpl20, gp41, N-terminal deletion variants (e.g. delta 1 1 deletions) as described herein, cleavage resistant variants, or codon optimized sequences thereof. Non-limiting examples of sequences are provided in Figures 39A-B, 40 A- C, and 41 A-C. The boost could be sequential or additive,

[0323] Selection I: V3 glycopeptide and/or aglycone peptide (SEQ ID NO: 1) as a prime; Boost: (Ί 1848.0949 10. 17. CH848.0358.80.06, (Ί 1848. 1432 5. 1. CH848.0526.25.02. See Figure 37A and Figure 28 in Example 1. [0324] Selection II: V3 glycopeptide and/or aglycone peptide as a prime; Boost:

(Ί 1848.0049. 10. 1 7: CH848.0836.10.31; CH848.0358.80.06; CH848.1432.5.41;

CH848.0526.25.02, See Figure 37A and Figures 27A-B, 18A-B, and 28 in Example 1.

[0325] Selection III: V3 glycopeptide and/or aglycone peptide as a prime; Boost:

CH848.0949, 10. 17; Π Ι848 d l 120. 10 21 : ( Ί 1848 d 1432.05 27 See Figure 3713.

[0326] Selection IV: V3 glycopeptide and/or aglycone peptide as a prime; Boost:

CH848.0949.10.17; CH848.dl 120.10.21 ; CH848d0949.10.18; ( Ί 1848x11432.05.27 See Figure 37C.

[0327] Selection V: V3 glycopeptide and/or aglycone peptide as a prime, Boost:

CH848.0949.10.17; CH848.0794.05. 4; CH848.0358.80.06; CH848.1432.5.41 ;

CH848.0526.25.09; CH848.0526.25.02,

[0328] Selection VI: CH848.0949.10.1 7 trimer as a prime, boost: CH848.0949.10.17;

optionally CH848.0836.10.31; CH848.0358.80.06; CH848.1432.5.41; CH848.0526.25.02.

[0329] Selection VII: CH848.0949.10.17 trimer as a prime, boost: C I 1848 0949 10. 1 7.

optionally CH848.0836.10.31; CH848.0358.80.06; CH848.1432.5.41; CH848.0526.25.02; CH0848.3.dl651.10.07.

[0330] In any of the above selections the prime could be selected from any of the

contemplated envelope designs that show binding to the DH270UCA.

[0331] In any one of the above selections, the boost could include CH848.dl305.10.13 and

CH0848.3.dl651.10.07 envelope designs to increase the breadth of antibodies.

0

[0332] This example describes additional considerations for selecting CH848 envelopes and modifications of such envelopes for use as immunogens.

[0333] CRF02 AG.T250 is an envelope which is very sensitive to V2glycan and V3glycan antibodies, and resistant to CD4bs antibodies. Short positively charged VI V2's are highly associated with sensitivity, and T250 has among the shortest VI V2 regions— So do

CH848.d0949.10.17 envelope. T250' s VI V2 region could be introduced in any of the envelopes describe herein, e.g. in CH0848.d0949.021 .10.17.

[0334] The best antibody from the Dl 1270 lineage is DH270.6. Like most V3 antibodies, it requires the N332 PNGS. In addition, D325N is highly associated with resistance, and is a common circulating mutation. Other V3glycan bNAbs can tolerate the mutation, and it arose in CH848 after DH270 lineage antibodies were isolated, likely possibly as an escape from our DH270 lineage. An N325 CH848 envelope isolate could be included in the vaccine to potentially extend breadth when DH270-like linages is started. There are several candidates, but only two had any binding or neutralizing activity CH848.dl305.10.13 and (Ί 10848. .d 1 65 1 . i 0.07 Figures 42,43, and 35, As CH848.dl 305.10. 13 has a proline after the N, GDIR -> GNPR, which is rare, CH0848.3.dl651.10.07 based envelopes are better vaccine option.

[0335] Figures 44A, 44B, and 44D show additional envelope designs, to introduce changes in the sequence of CH0848.3.D0949.10.17 to increase the sensitivity of these envelopes to antibodies in the DH270 lineage. Some of the changes affect glycans while others do not impact glycosyiation positions.

[0336] Table 3 provides a listing of reagents for use as prime(s) DH270 lineage germline binders and/or boosts (Amino acid sequences of these envelopes are provided in Figure 45).

Reference to amino acid positions is with respect to HXB2 envelope sequence.

2016;45(3):483-496. doi: 10.1016/j .immuni.2016.08.016.

VI glycans block PGT121 from binidng to the V3 glycan site (Garces et al. Cell. 2014 Sep 25; 159(l):69-79. dor 10.10 6/j.cell.2014.09.009; Garces et

B.JRFLg l40CF_

al. Immunity. 2015 Dec 15; 43(6): 1053-1063). A virus and an Env were V1_3Q

constructed where three potential N -linked sites in VI are deleted. The N is changed to Q hence 3Q.

VI glycans block PGT121 from binidng to the V3 glycan site (Garces et

CON-Sgpl40CFI_

alSupra). A vims and an Env were constructed where four potential reV1_4Q

linked sites in are VI deleted. The N is changed to Q hence 4Q.

CH848.3.D0949.10.1

Some bnAb precursor antibodies bind stronger to trimer than g i20 7CHIM.6R.SOSIP.66

monomers, autologous Env made as a stable trimer.

4V4.1

CH0848.3.D0836.10,

Some bnAb precursor antibodies bind stronger to trimer than gp!20 31CHIM.6R.SOSIP.6

monomers, autologous Env made as a stable trimer.

64V4.1

CH0848.3.D0358.80.

Some bnAb precursor antibodies bind stronger to trimer than gpl20

06CHIM.6R.SOSIP.6

monomers, autologous Env made as a stable trimer.

64V4.1

CH848.3.D1432.5.41

Some bnAb precursor antibodies bind stronger to trimer than gpl 20

CHIM.6R.SOSIP.664

monomers, autologous Env made as a stable trimer.

V4.1

CH848.3.D0526.25.0

Some bnAb precursor antibodies bind stronger to trimer than gpl 20

2CHIM.0R.SQSiP.60

monomers, autologous Env made as a stable trimer.

4V4.1

Mutations that were helpful in getting 1 IMUTB to bind to PGT121 were analyzed and similar mutations were constructed into CH848 D949.10.17

CH848.3.D0949.10.1

to create an Env that might bind to the DH270 UCA. Some bnAb

7CfflM.6R.SOSIP.66

precursor antibodies bind stronger to trimer than gpl 20 monomers thus we 4V4.1 _GT1

made the Env as a stable trimer. Also for immunization the native stable trimer has less exposure of nonneutralizing epitopes.

This construct is already published (Kong et al. (2013)

Nat.Struct.Mol.Biol. 2Θ: 796-803), but we have been treating the protein

B.JRFL

with deglycosylases under denaturing and non -denaturing conditions since gpl20core mini- the UCA of DH270 does not bind free glycan and may interfere with the V3_v2

UCA being able to bind to the base of the V3 loop. This is gpl20 with the variable loops 1 and 2 deleted. A truncated V3 loop remains.

CH848.3.D0949.10.1

Some bnAb precursor antibodies bind stronger to trimer than gpl 20 7chim.6R.DS.SOSIP.

monomers, autologous Env made as a stable trimer.

664 CH848.3.D0949.10.1

Some bnAb precursor antibodies bind stronger to trimer than gpl20 7chim.6R.DS.SOSIP.

monomers, autologous Env made as a stable trimer.

664

Some bnAb precursor antibodies bind stronger to trimer than gpl 20 monomers. This is an autologous Env made as a stable trimer. We then

CH848.3.D0949.10.1

removed the glycans at the base of the V3 loop since the UCA of DH270

7chmi.6R.DS.SOSIP.

does not bind free glycan and the glycans at N301 and N332 may interfere 664_N301AN332A

with the UCA being able to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

Some bnAb precursor antibodies bind stronger to trimer than gpl 20 monomers. This is an autologous Env made as a stable trimer. We then

CH848.3.D0949.10.1

removed the N332 glycan at the base of the V3 loop since the UCA of

7chmi.6R.DS.SOSIP.

DH270 does not bind free glycan and may interfere with the UCA being

664___N332A

able to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

Some bnAb precursor antibodies bind stronger to trimer than gpl 20 monomers. This is an autologous Env made as a stable trimer. We then

CH848.3.D0949.10.1

removed the N301 glycan at the base of the V3 loop since the UCA of

7chim.6R.DS.SOSIP.

DH270 does not bind free glycan and may interfere with the UCA being

664 N3Q1A

able to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

Some bnAb precursor antibodies bind stronger to trimer than gpl 20 monomers. This is an autologous Env made as a stable trimer. We then

CH848.3.D0949.10.1

removed the N301 glycan at the base of the V3 loop since the UCA of 7chim.6R.DS.SOSIP.

DH270 does not bind free glycan and may interfere with the UCA being

664 N301A

able to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

CH848.3.D0949.10.1 Long VI loops confers resistance to DH270 antibodies. Autologous Env 7chim.6R.DS.SOSIP. that started with a short VI loop and we replaced the loop with a

664 VIA GlySerGly linker.

CH848.3.D0949.10.1 Long VI loops confers resistance to DH270 antibodies. Autologous Env 7chim.6R.DS.SOSIP. that started with a short VI loop and we replaced the middle 1 1 amino

664_V1B acids of the VI loop with a GlySerGly linker.

CH848.3.D0949.10.1 Long VI loops confers resistance to D! 1270 antibodies. Autologous Env 7chim.6R.DS.SOSIP. that started with a short VI loop and we replaced the middle 7 amino acids

664_V1D of the VI loop with a GlySerGly linker.

Some bnAb precursor antibodies bind stronger to trimer than g l 20

CH848.3.D0949.10.1 monomers. Autologous Env made as a stable trimer. We then removed the CHIM.6R.SOSIP.66 N301 and N332 glycan at the base of the V3 loop since the UCA of

4V4.1degly4 DH270 does not bind free glycan and may interfere with the UCA being able to bind to the base of the V3 loop. V I glycans block PGT121 from binding to the V3 glycan site (Garces et al . see supra). So we made an Env with N137 and N141 potential glycosylation sites in VI removed. Glvcans were removed by mutation of the indicated amino acid position(s).

Some bnAb precursor antibodies bind stronger to trimer than gp!20 monomers. Autologous Env made as a uncleaved trimer. We then removed the N301 and N332 giycan at the base of the V3 loop since the UCA of

CH0848.3.D0949.10. DH270 does not bind free giycan and may interfere with the UCA being 17g l40C_degly4 able to bind to the base of the V3 loop. V I glvcans block PGT121 from binding to the V3 giycan site (Garces et al Immunity). So we made an Env with N137 and N 141 potential glycosylation sites in VI removed. Glvcans were removed by mutation of the indicated amino acid position(s).

28 VI glvcans block PGT121 from binidng to the V3 giycan site (Garces et al

Immunity). So we made a virus and an Env with three potential N-linked

BJRFL sites in V 1 deleted. The N is changed to Q hence 3Q. We then removed the gpl 40C_3QN301SN3 glvcans at the base of the V3 loop since the UCA of DH270 does not bind

32T free giycan and the glycans at N301 and 332 may interfere with the UCA being able to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

29 This is g l20 with the variable loops 1 and 2 deleted. A truncated V3 loop remains. We removed the N295A, N301 A, N332A glycans at the base of

BJRFL

the V3 loop since the UCA of DH270 does not bind free giycan and the gp!20core mini- glycans at N295A, N301 and N332 may interfere with the UCA being able V3_y2_degly

to bind to the base of the V3 loop. Glycans were removed by mutation of the indicated amino acid position(s).

[0337] Provided and contemplated are envelopes and modified version thereof for use as DH27G lineage germline binders:

[0338] Envelope (HV1301265) _JRFL gpl40 3QN301 SN332T -

[0339] Envelope CH848 703010848.3.d0949.10.17_signature_opt_filled_rare_holes_a_

CD5ss gpl40C. Contemplated is also a SOSIP design of the envelope CH848

703010848.3.d0949.10.17_signature_opt_filled_rare_holes_a_.

[0340] Envelope CH0848.3.dl651. 10.07 _CD5ss gpMOC

[0341] Envelope CH848 703010848.3.d0949.10.17_signature_opt_b_T250.4_V lV2_CD5ss gpl40C. Envelope CH848 703010848.3.d0949.10.17 signature opt b _CD5ss up HOC.

Contemplated is also a SOSIP design of this envelope,

[0342] Envelope T250-4 gp l40C

[0343] Envelope T250-4chim.6R. SOSIP.664v4.1 [0344] Envelope CH848 703010848.3.d0949.10.17_signature_opt_b_CD5ss gpl40C.

Contemplated is also a SOSIP design of this envelope,

[0345] Envelope CH848 703010848.3. d0949.10.17 signature opt filled rare holes a CD5ss gpMOC

[0346] Envelope CH8448 703010848.3. d0949.10.17 signature opt filled rare holes a CD5ss_N133AN138A

[0347] Envelope T250-4 gpl40C_N133AN138A -

[0348] Envelope J FL Core with miniV3 (293F produced/KIF treated/EndoH treated).

Example 3B

[0349] The example describes CH848 envelopes, trimers and additional envelopes, modifications and designs. This example shows that stabilized HIV-1 Env trimer

immunogens show enhanced antigenicity. See Figures 48A-48B. In some embodiments and are not recognized by non-neutralizing antibodies. In some embodiments these envelopes, including but not limited to trimers are further mutlimenzed, and/or used as particulate, high- density array in liposomes or other particles, for example but not limited to nanoparticles. Any one of the envelopes of the invention could be designed and expressed as described herein. The envelopes of the invention are engineered and tested for binding to various antibodies from the DH270 lineage.

[0350] Elicitation of neutralizing antibodies is one goal for antibody-based vaccines.

Neutralizing antibodies target the native trimeric HIV-1 Env on the surface virions. The trimeric HIV-1 envelope protein consists of three protomers each containing a gpl20 and gp41 heterodimer. Recent immunogen design efforts have generated soluble near-native mimics of the Env trimer that bind to neutralizing antibodies but not non-neutralizing antibodies. The recapitulation of the native trimer could be a key component of vaccine induction of neutralizing antibodies. Neutralizing Abs target the native trimeric HIV-1 Env on the surface of viruses (Poignard et al. J Virol. 2003 Jan;77(l):353-65; Parren et al. J Virol. 1998 Dec; 72(12): 10270-4.; Yang et al. J Virol. 2006 Nov;80(22): 11404-8.). The HIV-1 Env protein consists of three protomers of gpl20 and gp41 heterodimers that are noncovalentlv linked together (Center et al. J Virol. 2002 Aug;76(15):7863-7.). Soluble near-native trimers preferentially bind neutralizing antibodies as opposed to non-neutralizing antibodies (Sanders et al. PLoS Pathog. 2013 Sep; 9(9): el003618). [0351] Provided here are non-limiting embodiments of well-folded trimers or other engieneered forms of envelopes, which bind to the DH270UCA, and/or other DH270 lineage antibodies and are useful for Env immunizations as prime(s) and/or boosts.

[0352] Near-native soluble trimers using the 6R.SOSIP.664 design are capable of generating autologous tier 2 neutralizing plasma antibodies in the plasma (Sanders et al. 2015), which provides a starting point for designing immunogens to elicit broadly neutralizing antibodies. While these trimers are preferentially antigenic for neutralizing antibodies they still possess the ability to expose the V3 loop, which generally results in strain-specific binding and neutralizing antibodies after vaccination. Using the unliganded structure the

BG505.6R.SOSIP.664 has been stabilized by adding cysteines at position 201 and 433 to constrain the conformational flexibility such that the V3 loop is maintained unexposed (Kwon et al. Nat Struct Mol Biol. 2015 Jul; 22(7): 522-531.).

[0353] Immunogen design. Provided are engineered trimeric envelopes, for use as immunogens, wherein the envelopes are based on multiple viruses from CH848, and other viruses with suitable characteristics, e.g. VI loop length, as described.

[0354] We generated chimeric 6R.SOSIP.664, chimeric disulfide stabilized (DS)

6R.SOSIP.664 (Kwon et al Nat Struct Mol Biol. 2015 Jul; 22(7): 522-531.), chimeric 6R,SOSIP.664v4.1 (DeTaeye et al. Ceil. 2015 Dec 17; 163(7): 1702-15. doi:

10.1016/j .cell.2015.11.056), and chimeric 6R.SOSIP.664v4.2 (DeTaeye et al. Ceil. 2015 Dec 17; 163(7): 1702-15. doi: 10.1016/j . cell .20 i 5.11.056) . The 6R.SOSIP.664 is the basis for all of these designs and is made as a chimera of C.CH0505 and A.BG505. The gpl20 of C.CH848 was fused with the BG505 inner domain gpl20 sequence within the alpha helix 5 (a5) to result in the chimeric protein. The chimeric gpl20 is disulfide linked to the A.BG505 gp41 as outlined by Sanders et al. (PLoS Pathog. 2013 Sep; 9(9): el 003618). These immunogens were designed as chimeric proteins that possess the BG505 gp41 connected to the CH848 gp!20, since the BG505 strain is particularly adept at forming well-folded, closed trimers. This envelope design are expected to retain and expose features of the envelopes recognized by DH270 by broadly neutralizing antibody lineages that were isolated from CH848.

[0355] Figures 39A-B, 40A-C, and 41 A-C show nucleic acid and amino acid and sequences of various CH848 and other envelope trimer designs. Figure 41 C shows an annotated sequence of the SOSIP.III design. Based on the various SO SIP designs, any other suitable envelope, for example but not limited to CH848 envelopes as described in WO2015/153638 can be designed. [0356] Recombinant envelopes as trimers could be produced and purified by any suitable method. For a non-limiting example of purification methods see Ringe RP, Yasmeen A, Ozorowski G, Go EP, Pritchard LK, Guttman M, Ketas TA, Cottrell CA, Wilson IA, Sanders RW, Cupo A, Crispin M, Lee KK, Desaire H, Ward AB, Klasse PL Moore JP. 2015, Influences on the design and purification of soluble, recombinant native-like HIV-1 envelope glycoprotein trimers. J Virol 89: 12189 -12210, doi: 10.1128/JVI.01768-15.

[0357] Multimeric Envelopes

[ 0358] Presentation of antigens as prticulates reduces the B cell receptor affinity necessary for signal transduction and expansion (See Baptista et ai. EMBO J. 2000 Feb 15; 19(4): 513— 520). Displaying multiple copies of the antigen on a particle provides an avidity effect that can overcome the low affinity between the antigen and B cell receptor. The initial B cell receptor specific for pathogens can be low affinity, which precludes vaccines from being able to stimulate and expand B cells of interest. In particular, very few naive B cells from which HIV-1 broadly neutralizing antibodies arise can bind to soluble HIV-1 Envelope. Provided are envelopes, including but not limited to trimers as particulate, high-density array on liposomes or other particles, for example but not limited to nanoparticles. See e.g. He et al. Nature Communications 7, Article number: 12041 (2016), doi: 10.1038/ncommsl2041;

Bamrungsap et al. Nanomedicine, 2012, 7 (8), 1253-1271 ,

[0359] To improve the interaction between the naive B ceil receptor and CH848 SOSIP trimer protein we created to two constructs that can be presented on particles. The first construct was made by fusing HIV-1 Envelope trimer CH848 to ferritin. Ferritin protein self assembles into a small nanoparticle with three fold axis of symmetry. At these axis CH848 envelope protein was fused. Therefore the assembly of the three-fold axis also clusters three HIV-1 envelope protomers together to form an envelope trimer. Each ferritin particle has 6 axises which equates to 6 CH848 trimers being displayed per particle. See e.g. Sliepen et al. Retrovirology201512 : 82, DOI : 10.1186/s 12977-015 -0210-4.

[0360] Another approach to multimerize expression constructs uses staphylococcus Sortase A transpeptidase ligation to conjugate CH848 envelope trimers to cholesterol. The CH848 trimers can then be embedded into liposomes via the conjugated cholesterol. To conjugate the CH848 trimer to cholesterol either a C-teminal LPXTG tag or a N-terminal pentaglycine repeat tag was added to the CH505 envelope trimer gene. Cholesterol was also synthesized with these two tags. Sortase A was then used to covaiently bond the tagged CH505 envelope to the cholesterol. The sortase A-tagged trimer protein can also be used to conjugate the trimer to other peptides, proteins, or fluorescent labels, [0361] The invention provides design of envelopes and trimer designs wherein the envelope comprises a linker which permits addition of a lipid, such as but not limited to cholesterol, via a Sortase A reaction. See e.g. Tsukiji, S, and Nagamune, T. (2009), Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering. ChemBioChem, 10: 787-798. doi: 10. 1002/cbic.200800724; Proft, T. Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation. Biotechnol Lett (2010) 32: 1. doi : 10.1007/sl 0529-009-0116-0; Lena Schmohl, Dirk Schwarzer, Sortase- mediated ligations for the site-specific modification of proteins, Current Opinion in Chemical Biology, Volume 22, October 2014, Pages 122-128, ISSN 1367-5931,

dx.doi.org 10.1016/j.cbpa.2014.09.020; Tabata et ai. Anticancer Res. 2015 Aug;35(8):4411- 7.

[0362] The lipid modified envelopes and trimers could be formulated as liposomes. Any suitable liposome composition is contemplated.

[0363] Non-limiting embodiments of envelope designs for use in Sortase A reaction are shown in Figure 47B-C.

[0364] Design of trimers with readthrough codons

[0365] The development of clonal ceil lines that highly express trimeric HIV-1 Envelope will facilitate manufacturing of hig quality proteins for clinical and research purposes. However, it is challenging to identify the cells that express trimeric protein among the many cells making vari ous forms of HIV-1 Envelope with in the cell population. To identify cells expressing trimeric HIV-1 Envelope protein, we designed an expression construct that simultaneously produces both secreted Envelope protein as well as membrane anchored Envelope protein. The secreted Envelope protein can be purified using standard methods and results in unaltered soluble envelope. The membrane-anchored Envelope protein serves to mark the cells within a population of cells that expresses trimeric Envelope. More

specifically, the trimeric Envelope expressing cells are sorted by fluorescence-activated ceil sorting using a HIV-1 trimer specific antibody. The sorted cells can then be used to initiate clonal populations of cells that have been phenotypically shown to express the protein of interest.

[ 0366] The expression construct is designed by taking advantage of the amber stop codon UAG in messenger RNA. The codon UAG usually signifi es the end of the polypeptide sequence, but at a low rate the ribosome can readthrough this stop codon and continue to elongate the polypeptide chain. We incorporated this stop codon into our protein construct followed by the natural BG505 gp41 transmembrane and cytoplasmic tail sequence ended with two stop codons. Therefore, when the stop codon is readthrough a membrane-anchored gpl20/gp41 heterodimer is formed. Loughran et al. (Nucleic Acids Res. 2014

Aug;42(14):8928-38. doi : 10.1093/nar/gku608) identified that the efficiency of readthrough could be increased by flanking the amber stop codon with the nucleotides CTA. Readthrough could be even further augmented with the addition of CTAG nucleotides after the amber stop codon. We engineered expression constructs with both modifications to ensure an optimal ratio of membrane-anchored and secreted trimeric Envelope protein. Since the CTAG creates a shift in reading frame we added GC nucleotides after the CTAG motif to preserve the original reading frame. The addition of CTAGGC results in the membrane anchored protein having a leucine and glycine residue expressed before the transmembrane domain. Any one of the envelopes of the invention could be designed and expressed as readthrough envelopes. Example 4: Animal studies

[0367] Various selections of immunogens will be tested in animal models. Any suitable animal model will be used. Such animal models include mouse models, including humanized mice carrying human immunoglobulin locus, guinea pigs, rabbits, non-human primates, or any other model. Adults and neonates could be used in the studies.

[0368] Mouse study: prime with Man9 V3 (SEQ ID NO: 1) glycan monomer with adjuvant LASTS. Boost at least twice with CH848 d0949.10.17Δ11 gpl20 with adjuvant, e.g.LASTS.

[0369] Mouse study: prime with V3 (SEQ It) NO: 1) aglycan monomer with adjuvant LASTS. Boost at least twice with CH848 d0949.1Q.17Al 1 gpl20 with adjuvant LASTS.

[0370] In the immunogenic methods of the invention, the first boost after the prime comprises CH848 d0949.10.17 envelope either as a protein or nucleic acid in any suitable

[0371] The adjuvant in the above studies could be any suitable adjuvant, for example but no limited to polylC or polylC/LC.

Claims

CLAIMS What is claimed is:

1. A recombinant HIV-1 envelope polypeptide, wherein the polypeptide comprises the amino acid sequence of any one of the envelopes designs in Table 1, Table 3 or Example 3

2. A recombinant HIV-1 envelope polypeptide from Table 1, Table 3 or Example 3

wherein the polypeptide is n on -naturally occurring and designed to form a soluble trimer.

3. A nucl eic acid encoding any one of the polypepti des of claim 1 or 2,

4. An immunogenic composition comprising the recombinant HIV-1 envelope polypeptide of claim 1 or claim 2 and a carrier,

5. An immunogenic composition comprising the nucleic acid of claim 3 and a carrier,

6. The composition of claim 4 or 5 further comprising an adjuvant,

7. The composition of claim 3 or 5 wherein the nucleic acid is operably linked to a

promoter inserted in an expression vector.

8. A method of inducing an immune response in a subject comprising administering a composition comprising an HIV-1 envelope polypeptide(s) in an amount sufficient to induce an immune response from one or more of the following groups:

(a) V3 glycopeptide, V3 aglycone, envelope polypeptide(s) designed to bind

DH270UCA (Table 1, Table 3, Ex, 3), or any combination thereof as a prime,

(b) envelope polypeptide(s) CH848.0949.10.17, CH848.0836.10.31,

CH848.0358.80.06; CH848.1432.5.41; CH848.0526.25,02 (Figure 37A), or any combination thereof;

(c) envelope polypeptide CH0848.3.dl651.10.07;

9. and wherein the administration step can alternatively, or in addition, comprise

administering any suitable form of a nucleic acid(s) encoding an HIV-1 envelope polypeptide! s) in an amount sufficient to induce an immune response from one or more of the following groups:

(a) envelope polypeptide(s) designed to bind DH270UCA (Table 1, Table 3, Ex. 3), or any combination thereof as a prime;

(b) envelope polypeptide(s) (Ί 1848.0949, 10 1 7. CH848.0836.10.31 ,

CH848.0358.80.06; CH848.1432.5.41; CH848.0526.25.02 (Figure 37 A), or any combination thereof;

(c) envelope polypeptide CH0848.3.dl 651.10.07.

10. The method of claim 8 wherein the nucleic acid encodes a gpl20 envelope, gpl 20Dl 1 envelope, a gp !40 envelope (gpl40C, gpl40CF, gpMOCFI) as soluble or stabilized protomer of a SOSIP trimer, a gpl45 envelope, a gp l 50 envelope, or a transmembrane bound envelope.

1 1 . The method of claim 8 wherein the polypeptide is gpl20 envelope, gpl20D l envelope, a gpl40 envelope (gpMOC, gpMOCF, gp HOCFI) as soluble or stabilized protomer of a SOSIP trimer, a gp! 45 envelope, a gpl SO envelope, or a transmembrane bound envelope.

12. The method of claim 8, wherein the composition further comprises an adjuvant.

13. The method of claim 8, further comprising administering an agent which modulates host immune tolerance.

14. The method of claim 8, wherein the polypeptide admini stered is multimerized in a

liposome or nanoparticle.

15. The method of claim 8, further comprising administering one or more additional HIV-l immunogens to induce a T cell response.

16. The method of claim 8, wherein the first boost administered after the prime comprises HIV-l envelope polypeptide CH848.0949.10.17 in any suitable form.

17. The recombinant HIV-l envelope polypeptide of claim 2, wherein the polypeptide does not include a signal peptide.