WO2021218537A1 - 检测芯片及检测系统 - Google Patents

检测芯片及检测系统 Download PDF

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Publication number
WO2021218537A1
WO2021218537A1 PCT/CN2021/084007 CN2021084007W WO2021218537A1 WO 2021218537 A1 WO2021218537 A1 WO 2021218537A1 CN 2021084007 W CN2021084007 W CN 2021084007W WO 2021218537 A1 WO2021218537 A1 WO 2021218537A1
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WIPO (PCT)
Prior art keywords
detection
substrate
groove
sample
detection chip
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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PCT/CN2021/084007
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English (en)
French (fr)
Inventor
赵静
张玙璠
袁春根
安光明
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BOE Technology Group Co Ltd
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BOE Technology Group Co Ltd
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Application filed by BOE Technology Group Co Ltd filed Critical BOE Technology Group Co Ltd
Priority to KR1020227016412A priority Critical patent/KR20230005803A/ko
Priority to US17/626,317 priority patent/US20220241774A1/en
Priority to JP2022540535A priority patent/JP7791821B2/ja
Priority to EP21795526.9A priority patent/EP4067907A4/en
Publication of WO2021218537A1 publication Critical patent/WO2021218537A1/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • G—PHYSICS
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    • G01N35/00069—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
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    • G01N35/00009—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with a sample supporting tape, e.g. with absorbent zones
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    • B01L2200/02—Adapting objects or devices to another
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    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/0627—Sensor or part of a sensor is integrated
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    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00108—Test strips, e.g. paper
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N2035/00099—Characterised by type of test elements
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    • G01N2035/00178—Special arrangements of analysers
    • G01N2035/00237—Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/00584—Control arrangements for automatic analysers
    • G01N35/00594—Quality control, including calibration or testing of components of the analyser
    • G01N35/00693—Calibration

Definitions

  • the embodiment of the present disclosure relates to a detection chip and a detection system.
  • Microfluidic chip technology integrates the basic operation units of sample preparation, reaction, separation, and detection involved in the fields of biology, chemistry, and medicine into a chip with micrometer-scale microchannels to automatically complete the entire process of reaction and analysis.
  • the chip used in this process is called a microfluidic chip, which can also be called a Lab-on-a-chip (Lab-on-a-chip).
  • Microfluidic chip technology has the advantages of small sample consumption, fast analysis speed, easy to make portable instruments, and suitable for instant and on-site analysis. It has been widely used in many fields such as biology, chemistry and medicine.
  • At least one embodiment of the present disclosure provides a detection chip that includes a sample opening and at least one detection branch structure; each of the at least one detection branch structure includes a detection portion, and the detection portion includes a detection groove and a reaction reagent , Wherein the detection groove is in communication with the sample addition opening, the reaction reagent is contained in the detection groove, and the detection portion is configured to allow the reaction reagent located in the detection groove Perform optical inspection.
  • each of the at least one detection branch structure further includes a diversion groove, the diversion groove having a first end and a second end, and the diversion groove The first end of the groove is in communication with the sampling opening, and the second end of the diversion groove is in communication with the detection groove, so that the detection groove is in communication with the sampling opening through the diversion groove .
  • the detection chip provided by at least one embodiment of the present disclosure further includes: a first substrate having a first surface, wherein the sample adding opening is a through hole in the first substrate, the flow guide groove and the A detection groove is formed on the first surface of the first substrate; a second substrate is laminated on the first surface of the first substrate and allows the optical detection at a position corresponding to the detection groove.
  • each of the at least one detection branch structure further includes a water-absorbing film, and the water-absorbing film is contained in the detection groove and interacts with the reaction reagent. At least partially stacked with each other in a direction perpendicular to the first substrate.
  • the water-absorbing film is disposed on a side of the reaction reagent away from the second substrate.
  • the liquid storage capacity of the water-absorbing film is 10 ⁇ L-50 ⁇ L.
  • the detection portion further includes a diversion groove located at the periphery of the detection groove, and the diversion groove is in communication with the detection groove;
  • the height of the diversion groove is smaller than the height of the detection groove.
  • the diversion groove includes a slope-shaped diversion wall, and one end of the diversion wall is in contact with a side surface of the detection groove.
  • the entire longitudinal section of the flow guiding groove and the detection groove are stepped.
  • the flow guiding groove at least partially surrounds the detection groove.
  • the height of the detection groove is 0.2mm-5mm, and the difference between the maximum height of the diversion groove and the height of the detection groove is 0.1 mm-1mm, the width of the diversion groove is 0.1mm-1mm.
  • the detection portion further includes a liquid storage through hole that penetrates the first substrate and communicates with the detection groove.
  • the liquid storage through hole communicates with the center of the detection groove.
  • the diameter of the liquid storage through hole is 0.2mm-5mm, and the ratio of the depth of the liquid storage through hole to the height of the detection groove is 0.5: 1-10:1.
  • the height of the diversion groove is 0.1 mm-1.5 mm, and the width of the diversion groove is 0.1 mm-2 mm.
  • the ratio of the height of the diversion groove to the width of the diversion groove is 1:1-10:1.
  • the inner wall of the diversion groove is hydrophilic.
  • the at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample addition opening.
  • the sample addition opening includes a first body and a first protrusion protruding from the first body toward the diversion groove, and the first The protruding part communicates with the diversion groove.
  • the diameter of the first body is 1 mm-10 mm.
  • the reaction reagent includes a reaction membrane and/or a block-shaped reaction reagent.
  • the reaction film is in a compressed state along the thickness direction.
  • the ratio of the thickness of the reaction film in a relaxed state to the height of the detection groove is 1:1 to 1:0.5.
  • the reaction membrane is circular, or the reaction membrane is polygonal, and one corner of the polygon is directly connected to the second end of the diversion groove .
  • the reaction membrane includes a membrane main body and a second protrusion protruding from the membrane main body toward the diversion groove, and the second protrusion At least part of is located in the diversion groove; the film body and the detection groove have the same shape and are circular.
  • the reaction film is diamond-shaped.
  • the diameter of the detection groove is 3 mm-15 mm, and the diameter of the reaction film is equal to or smaller than the diameter of the detection groove.
  • the second substrate has a detection through hole at a position corresponding to the detection groove.
  • the second substrate does not transmit light; or, the detection chip further includes a light shielding layer that covers the second substrate away from and/or close to The surface of the first substrate exposes the detection through hole.
  • the diameter of the detection through hole is 2 mm-10 mm.
  • the material of the first substrate includes one or more of polymethyl methacrylate, polystyrene, and polycarbonate.
  • the material of the second substrate includes one of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate. kind or more.
  • the first substrate and the second substrate are combined by bonding, welding, bonding or clamping.
  • the detection chip provided by at least one embodiment of the present disclosure further includes an adhesive layer; wherein the adhesive layer is located between the first substrate and the second substrate and is used to make the first substrate and the The second substrate is combined, and the adhesive layer includes an opening corresponding to the detection groove.
  • the reaction reagent includes a matrix material and a detection reagent distributed in the matrix material, and the matrix material includes glass fiber, cotton fiber, or glass fiber and cotton fiber. Of composite fibers.
  • the detection chip provided by at least one embodiment of the present disclosure further includes: a first substrate having a first surface, wherein the sample adding opening is a through hole in the first substrate, the flow guide groove and the A detection groove is formed on the first surface of the first substrate; a second substrate is laminated on the first surface of the first substrate and has a detection through hole at a position corresponding to the detection groove to allow The optical detection is performed through the detection through hole; the at least one detection branch structure includes a plurality of detection branch structures, and the plurality of detection branch structures are evenly distributed along the periphery of the sample adding opening.
  • the detection chip provided by at least one embodiment of the present disclosure further includes: a first substrate having a first surface and a second surface opposite to each other, wherein the sample loading opening is a through hole in the first substrate, and The detection groove is formed on the first surface of the first substrate, and the diversion groove is formed on the second surface of the first substrate; the second substrate is laminated on the first surface of the first substrate and is on the The position corresponding to the detection groove allows the optical detection; a third substrate is laminated on the second surface of the first substrate and seals the sample addition opening and the guide groove.
  • the detection chip provided by at least one embodiment of the present disclosure further includes: a first substrate having a first surface, wherein the sample application opening, the flow guide groove, and the detection groove are formed on the first substrate
  • the first surface of the sample application opening is a non-through hole in the first substrate
  • the second substrate is laminated on the first surface of the first substrate and exposes the sample application opening, and corresponds to The position of the detection groove allows the optical detection.
  • the detection chip provided by at least one embodiment of the present disclosure further includes an optical calibration branch structure, wherein the optical calibration branch structure includes an optical path detection area, and the optical path detection area is configured to perform optical calibration.
  • the detection chip provided by at least one embodiment of the present disclosure further includes a sample application protrusion: wherein the sample application protrusion protrudes from the first surface of the first substrate in a direction away from the second substrate, One end of the sample adding protrusion is connected with the sample adding opening.
  • the sample addition protrusion includes a conical cavity, the volume of the conical cavity is 50 ⁇ L-200 ⁇ L, and the conical cavity protrudes from the The height of the first substrate is 1mm-20mm; the conical cavity includes a first end and a second end opposite to each other, the first end is connected to the sample opening, and the diameter of the first end is 0.5mm-5mm, the diameter of the second end is 1mm-20mm.
  • the first substrate includes a first notch
  • the second substrate includes a second notch corresponding to the first notch
  • the first The notch and the second notch are used to fix the detection chip.
  • At least one embodiment of the present disclosure further provides a detection system, including: a detection device and the detection chip according to any embodiment of the present disclosure; the detection device is configured to react to the detection in the detection groove through the detection portion Reagents for testing.
  • the detection device includes: a light source configured to emit light to the reaction reagent; Reflected light.
  • FIG. 1A and 1B are respectively a front perspective view and a back perspective view of a detection chip provided by at least one embodiment of the present disclosure
  • Fig. 2A and Fig. 2B are respectively exploded views of the detection chip shown in Fig. 1A and Fig. 1B;
  • 3A and 3B are respectively a front perspective view and a back perspective view of the first substrate of the detection chip shown in FIGS. 1A and 1B;
  • FIGS. 1A and 1B are schematic plan views of the first surface of the first substrate of the detection chip shown in FIGS. 1A and 1B;
  • FIGS. 1A and 1B are schematic plan views of the second substrate of the detection chip shown in FIGS. 1A and 1B;
  • FIG. 6 is a schematic diagram of the structure of the reaction film of the detection chip shown in FIG. 1A and FIG. 1B;
  • 7A and 7B are respectively a front perspective view and a back perspective view of another detection chip provided by at least one embodiment of the present disclosure
  • Fig. 8 is an exploded view of the detection chip shown in Figs. 7A and 7B;
  • 9A and 9B are respectively a front perspective view and a back perspective view of the first substrate of the detection chip shown in FIGS. 7A and 7B;
  • FIGS. 7A and 7B are schematic plan views of the first surface of the first substrate of the detection chip shown in FIGS. 7A and 7B;
  • FIG. 11 is an exploded view of still another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 12 is a schematic diagram of the structure of the first substrate of the detection chip shown in FIG. 11;
  • FIG. 13A is a schematic diagram of a part of the structure of the first substrate of the detection chip shown in FIG. 12;
  • Fig. 13B is a schematic cross-sectional structure view taken along the line A-A' in Fig. 13A;
  • Fig. 13C is a schematic cross-sectional structure view taken along the line B-B' in Fig. 13A;
  • FIG. 14 is an exploded view of still another detection chip provided by at least one embodiment of the present disclosure.
  • 15A and 15B are exploded views of yet another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 16 is an exploded view of still another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 17 is a schematic diagram of a detection system provided by at least one embodiment of the present disclosure.
  • the inventor hopes to integrate the various functions of analysis and detection on the chip as much as possible, so as to reduce the chip's dependence on external operations, thereby achieving automation and integration.
  • At least one embodiment of the present disclosure provides a detection chip, which includes a sample addition opening and at least one detection branch structure.
  • Each of the at least one detection branch structure includes a detection portion; the detection portion includes a detection groove and a reaction reagent, the detection groove is in communication with the sample injection opening, the reaction reagent is contained in the detection groove, and the detection portion is configured to allow the alignment detection The reaction reagent in the groove is optically detected.
  • the detection chip provided by at least one embodiment of the present disclosure can integrate multiple basic structural units or components for sample detection on the same chip, and complete the whole process of sample analysis and detection through active control or capillary action, thereby realizing automation and Integrated inspection process. Therefore, the use of the above detection chip can reduce possible human errors in the detection process, improve the accuracy of the detection data, and at the same time enable the overall shape of the detection chip to achieve a thin or miniaturized design, which is helpful to realize a portable detection system .
  • each of the at least one detection branch structure further includes a diversion groove, the diversion groove has a first end and a second end, and the first end of the diversion groove is in communication with the sample addition opening, and the diversion groove has a first end and a second end. The second end of the flow groove communicates with the detection groove, so that the detection groove communicates with the sample injection opening through the flow guide groove.
  • FIGS. 1A and 1B are respectively a front perspective view and a back perspective view of a detection chip provided by at least one embodiment of the present disclosure.
  • FIGS. 2A and 2B are respectively exploded views of the detection chip shown in FIG. 1A and FIG. 1B. That is, the exploded view.
  • Figures 1A and 2A are top views of the detection chip, showing the structure viewed from the front of the detection chip;
  • Figures 1B and 2B are bottom views of the detection chip, showing the structure viewed from the back of the detection chip.
  • the detection chip 10 includes a sample addition opening 110 and at least one detection branch structure 120, such as a plurality of detection branch structures 120, and six detection branch structures 120 are shown as examples for introduction.
  • the sample adding opening 110 is used to add a sample to be tested, such as breast milk, body fluids, blood, and other samples to be tested.
  • the plurality of detection branch structures 120 are evenly distributed along the periphery of the sample adding opening 110, so that the plurality of detection branch structures 120 can implement detection functions independently.
  • each of the plurality of detection branch structures 120 includes the diversion groove 130 and the detection part 140.
  • the diversion groove 130 has a first end and a second end, and the first end is in communication with the sample injection opening 110.
  • the detection part 140 includes a detection groove 141 and a reaction reagent 150, the detection groove 141 is in communication with the second end of the diversion groove 130, and the reaction reagent 150 is contained in the detection groove 141.
  • the detection part 140 is configured to allow optical detection of the reaction reagent 150 in the detection groove 141.
  • the reaction reagent 150 may be in the shape of a membrane as shown in FIGS. 1A-2B, that is, a reaction membrane; or, in some embodiments, the reaction reagent 150 may be in the form of a block or powder.
  • the reaction reagent for example, can be a block or powdered freeze-dried reagent, etc.; or, in some embodiments, for example, when the detection chip includes multiple detection branch structures, it can also be a reaction contained in a part of the detection groove
  • the reagent is a membrane-shaped reaction membrane, and the reaction reagent contained in another part of the detection groove is a block or powder reaction reagent that is different from the membrane-like reaction membrane, which is not limited in the embodiments of the present disclosure.
  • reaction reagent 150 as a reaction film (ie, the reaction film 150)
  • the detection chip provided in the embodiment of the present disclosure. It should be noted that the embodiments of the present disclosure include but are not limited to this.
  • the detection chip further includes an optical calibration branch structure
  • the optical calibration branch structure includes an optical path detection area
  • the optical path detection area is configured to perform optical calibration, thereby improving the accuracy and accuracy of detection data obtained through optical detection. Accuracy.
  • the optical calibration branch structure and the plurality of detection branch structures are evenly distributed along the periphery of the sample application opening.
  • No reaction film is provided in the optical alignment branch structure.
  • other structures of the optical alignment branch structure except the reaction film can be basically the same as or similar to the detection branch structure, so the optical alignment branch structure can be integrated with the detection branch structure to simplify The preparation process and preparation cost of the detection chip.
  • a detection branch structure 120 may not be provided with a reaction film 150 and it can be used as an optical calibration branch structure of the detection chip 10 for optical calibration, for example,
  • the reaction film 150 (and the water-absorbing film 170 described below) is not provided, and it is used as the optical alignment branch structure of the detection chip 10.
  • the optical alignment branch structure includes a light path detection area, and the light path detection area may be a position corresponding to the detection groove 141. Therefore, when the detection device is used to perform optical detection on the reaction film 150 in the detection chip 10, the light path emitted by the detection device can be calibrated through the optical calibration branch structure, for example, the irradiation angle of the light path is calibrated, thereby improving the detection obtained. The accuracy of the data.
  • two or more optical alignment branch structures may also be provided for optical alignment.
  • the two detection branch structures 120 can be used as the optical alignment branch structure of the detection chip 10 without disposing, for example, the reaction film 150.
  • the branch structure may be symmetrical with respect to the center of the sample addition opening 110, thereby improving the accuracy and precision of the optical calibration, and further improving the accuracy of the obtained detection data.
  • the detection chip can also be calibrated in other ways according to actual needs.
  • the calibration branch structure can also be other types of calibration structures other than optical calibration, such as
  • reaction membranes can be provided according to actual needs, or no reaction membranes (or other structures or components that may affect calibration, such as the water-absorbing membrane 170 described below) are provided, according to the embodiments of the present disclosure. There is no restriction on this.
  • the reaction membrane 150 includes a detection reagent; the sample to be tested added from the sample addition opening 110 enters the detection groove 141 through the flow guide groove 130 and reacts with the detection reagent in the reaction membrane 150.
  • optical detection can be used to detect the color change reflected on the reaction film 150 after the reaction between the detection reagent and the sample to be tested, so as to realize the detection of the sample to be tested, for example, the presence of a certain component in the sample to be tested. No or the size of the content, etc.
  • the detection chip 10 provided by at least one embodiment of the present disclosure can realize the integration of multiple functions such as mixing, analysis and detection, and complete the whole process of sample analysis and detection through active control and capillary action, thereby achieving automation and integration.
  • the detection process reduces the human error that may exist in the detection process and improves the accuracy of the detection data.
  • the detection chip 10 may respectively place a reaction film 150 including different detection reagents in the detection grooves 141 of the plurality of detection branch structures 120, so that different detection reagents can be used to detect multiple indicators in the same sample. At the same time, the detection is carried out to shorten the detection cycle of the sample, and then realize the timely detection of multiple indicators of the sample.
  • the sample to be tested may be a liquid, such as a breast milk sample.
  • the required detection reagents (such as color reagents) can be pre-titrated on the initial reaction film 150. After the detection reagents are immersed in the reaction film 150, they are dried, and then the reagents including the detection reagents are dried. The reaction film 150 is placed in the detection groove of the detection chip 10, thereby facilitating the storage and transportation of the detection chip 10.
  • the detection chip 10 is used for detection, the sample is added from the sample addition opening 110, and the sample flows into the detection groove 141 through the diversion groove 130 and is mixed with the detection reagent on the reaction membrane 150 to react.
  • the reaction result for example, by identifying the color change on the reaction film 150
  • the presence or absence of the analyte in the sample and the size of the content can be judged, so as to realize the detection of a certain index of the sample.
  • the amount of sample injected when the detection chip 10 is used to detect a sample, the amount of sample injected may be 60 ⁇ L-80 ⁇ L. As a result, the amount of sample required when the detection chip 10 is used to detect the sample can be reduced, thereby reducing or avoiding sample waste.
  • each detection branch structure 120 may further include a water-absorbing film 170.
  • the water-absorbing film 170 is contained in the detection groove 141 and is in contact with the reaction film 150 in the reaction film 150.
  • the thickness direction that is, in the direction perpendicular to the first substrate 101 described below
  • the two are in direct contact with each other.
  • each water-absorbing film 170 may be 10 ⁇ L-50 ⁇ L, for example, 30 ⁇ L.
  • the water absorbing film 170 can hold a certain amount of samples. After the liquid storage capacity of the water absorbing film 170 is exceeded, the water absorbing film 170 will no longer absorb the sample, thereby playing a role of quantifying the sample and helping to control the amount of the sample.
  • the material of the water-absorbing film 170 may include materials such as glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber.
  • the detection chip may not use a water-absorbing film.
  • the reaction film 150 can simultaneously serve as a sample quantification and a reaction carrier.
  • the length, width, and depth of the plurality of diversion grooves 130 are the same as each other, so that the amount of sample flowing into different detection grooves 141 can be controlled to be relatively uniform, which is beneficial to The amount of sample in the detection groove 141 is controlled, and at the same time, the reaction time between the sample in the different detection groove 141 and the detection reagent on the reaction film 150 can be kept relatively consistent, thereby improving the accuracy of the detection data obtained.
  • the multiple diversion grooves 130 may be used to detect different indicators of the sample to be detected.
  • the specific length, width, and depth of the diversion groove 130 may be determined according to actual requirements such as sample amount and sample characteristics.
  • the length of the flow guide groove 130 can be appropriately reduced to reduce or avoid the waste of the sample in the flow process.
  • the diversion groove 130 may not be provided in the detection chip 10, for example, the detection groove 141 is directly connected to the sample addition opening 110 to achieve communication, thereby further reducing or avoiding sample loading. Waste in the flow process.
  • the sample adding opening 110 may have a flow guiding structure respectively connected with the plurality of detection grooves 141, so that the sample added into the sample adding opening 110 can evenly flow into the plurality of detection grooves 141.
  • the size of the multiple detection grooves 141 can also be selected according to different indicators of the sample to be detected, the amount of the sample required, and the type of detection reagent. According to actual requirements, the size and shape of the multiple detecting grooves 141 may be the same or different, which is not limited in the embodiment of the present disclosure.
  • the detection chip 10 further includes a first substrate 101 and a second substrate 102.
  • the first substrate 101 has a first surface and a second surface opposite to each other.
  • the sample application opening 110 is a through hole in the first substrate 101, and the diversion groove 130 and the detection groove 141 are formed on the first surface of the first substrate 101.
  • the second substrate 102 is laminated on the first surface of the first substrate 101 and allows optical detection at a position corresponding to the detection groove 141.
  • FIGS. 3A and 3B are respectively a front perspective view and a back perspective view of the first substrate of the detection chip shown in FIGS. 1A and 1B.
  • 3A is a top view of the first substrate 101, showing the front structure of the first substrate
  • FIG. 3B is a bottom view of the first substrate 101, showing the back structure of the first substrate.
  • 4 is a schematic plan view of the first surface of the first substrate of the detection chip shown in FIGS. 1A and 1B
  • FIG. 5 is a schematic plan view of the second substrate of the detection chip shown in FIGS. 1A and 1B.
  • the water absorption film 170 may be disposed on the side of the reaction film 150 away from the second substrate 102, that is, on the reaction film 150 and the detection groove 141. between.
  • the second substrate 102 has a detection through hole 160 at a position corresponding to the detection groove 141, and the reaction film 150 in the detection groove 141 can be optically detected through the detection through hole 160 to obtain the detection index of the sample.
  • the detection through hole 160 penetrates the second substrate 102, and the diameter of the detection through hole 160 may be set to 2 mm-10 mm, further may be 0.5 mm-4 mm, for example, may be set to 3 mm.
  • optical detection can be achieved through the detection through holes 160, and interference between light rays directed to different detection through holes 160 can be reduced or avoided, thereby further improving the accuracy and precision of optical detection.
  • all parts of the second substrate 102 other than the detection through holes 160 can be set as opaque parts, thereby reducing or avoiding interference between light rays directed to different detection through holes 160, and reducing or avoiding detection through Optical crosstalk between holes 160.
  • the opaque portion of the second substrate 102 can be formed by dyeing or the like.
  • a light-shielding layer can also be provided on the side of the second substrate 102 away from the first substrate 101, and the light-shielding layer covers the surface of the second substrate 102 away from the first substrate 101 except for the position with the detection through hole 160.
  • the light shielding layer covers the surface of the second substrate 102 away from the first substrate 101 and exposes the detection through holes 160, so as to also reduce or avoid interference between light rays directed to different detection through holes 160, and reduce or avoid detection Optical crosstalk between vias 160.
  • the light-shielding layer is made of an opaque material, and the light-shielding layer can be disposed on the surface of the second substrate 102 by means of, for example, pasting, printing, etc. The embodiment of the present disclosure does not limit the specific manner of forming the light-shielding layer.
  • the above-mentioned light-shielding layer may also be provided on the side of the second substrate 102 close to the first substrate 101, that is, the light-shielding layer covers the surface of the second substrate 102 close to the first substrate 101 and exposes the detection through hole 160, so that it can also weaken or It is avoided that the light rays directed to different detection through holes 160 interfere with each other.
  • the side of the second substrate 102 away from the first substrate 101 and the side of the second substrate 102 close to the first substrate 101 may both be provided with a light-shielding layer, the two light-shielding layers respectively Cover other parts of the surface of the second substrate 102 far away from and close to the first substrate 101 except for the position of the detection through hole 160, thereby better reducing or avoiding the occurrence of light rays directed to different detection through holes 160. Interference, thereby reducing or avoiding the optical crosstalk between the detection vias 160.
  • the detection through hole may not be provided on the second substrate of the detection chip.
  • the second substrate may be made of a transparent material that allows light to pass through, so that it can directly pass through the second substrate.
  • the reaction film in the detection groove is optically detected.
  • the second substrate may be made of a transparent material that allows light to pass through, and a detection window is formed by printing or pasting an opaque light shielding layer on the surface of the second substrate away from the first substrate to allow the light to pass only through the detection window.
  • the light shielding layer may also be provided on the surface of the second substrate on the side close to the first substrate, or on the surface of the second substrate on the side close to the first substrate and on the side far away from the first substrate.
  • a light-shielding layer may be provided on the surface at the same time, which is not limited in the embodiments of the present disclosure.
  • the embodiment of the present disclosure does not limit the specific material used for the second substrate, as long as it satisfies that the reaction film in the detection groove of the first substrate can be optically detected through the second substrate or the detection through hole opened on the second substrate.
  • the number of detection grooves 141 provided on the first substrate 101 and the number of detection through holes 160 corresponding to the detection grooves 141 provided on the second substrate 102 are merely exemplary.
  • the disclosed embodiment does not limit the specific number of the detection groove 141 and the detection through hole 160.
  • 1-20 detection grooves 141 may be provided on the first substrate 101, and correspondingly, 1-20 detection through holes 160 may be provided on the second substrate 102.
  • the material of the second substrate 102 may include one or more of polymethyl methacrylate, polystyrene, polycarbonate, and polyethylene terephthalate.
  • the material of the first substrate 101 may include one or more of polymethyl methacrylate, polystyrene, and polycarbonate, or may also be other materials with high light transmittance.
  • the embodiment of the present disclosure does not specifically limit the materials of the first substrate 101 and the second substrate 102.
  • the first substrate 101 and the second substrate 102 can be joined by bonding (referring to a technology in which two pieces of materials are bonded into one body through van der Waals force, molecular force, and atomic force), welding, bonding, or clamping.
  • the combination further simplifies the processes such as processing and assembly of the detection chip 10 and reduces the manufacturing cost of the detection chip 10.
  • the first substrate 101 and the second substrate 102 may be combined through processes such as ultrasonic bonding, thermal compression bonding, laser welding, ultrasonic welding, or silicone sealing.
  • the embodiment of the present disclosure does not limit the specific bonding manner between the first substrate 101 and the second substrate 102.
  • the shape of the first substrate 101 may be a circle, a rectangle or other suitable shapes
  • the shape of the second substrate 102 may also be a circle, a rectangle or other suitable shapes.
  • the diameter of the first substrate 101 may be 3 cm-15 cm, and further may be 3 cm-5 cm. Since the detection chip 10 needs to be matched with, for example, a detection device during use, the first substrate 101 of the above-mentioned size can reasonably reduce the space required for the detection chip 10, so that the detection chip 10 can be designed with a thinner profile, which is helpful for detection.
  • the chip 10 is used in conjunction with the detection device, and the cost of transportation, packaging, and storage of the detection chip 10 is reduced.
  • the diameter of the second substrate 102 may be 3 cm-15 cm, and further may be 3 cm-5 cm.
  • the second substrate 102 of the above-mentioned size can reasonably reduce the space required for the detection chip 10, so that the detection chip 10 can be designed with a thinner profile, thereby facilitating the detection.
  • the chip 10 is used in conjunction with the detection device, and the cost of transportation, packaging, and storage of the detection chip 10 is reduced.
  • the diagonal of the first substrate 101 may be 3 cm-15 cm, and further may be 3 cm-5 cm.
  • the rectangular first substrate 101 can simplify the preparation process of the detection chip 10, reduce the processing technology and accuracy requirements of the detection chip 10, and thereby reduce the preparation cost of the detection chip 10.
  • the diagonal of the second substrate 102 may be 3 cm-15 cm, and further may be 3 cm-5 cm.
  • the rectangular second substrate 102 can simplify the preparation process of the detection chip 10, reduce the processing technology and accuracy requirements of the detection chip 10, and thereby reduce the preparation cost of the detection chip 10.
  • the shape and size of the first substrate 101 and the second substrate 102 may be the same or similar to each other, so as to facilitate the combination of the first substrate 101 and the second substrate 102.
  • the thickness of the first substrate 101 may be 0.5 mm-10 mm, such as 3 mm or 5 mm.
  • the thickness of the second substrate 102 may be 0.1 mm-5 mm, such as 0.5 mm or 2 mm.
  • the space required for the detection chip 10 can be reasonably reduced, and the detection chip 10 can realize a thin design, thereby reducing the cost of transportation, packaging, and storage of the detection chip 10, and is helpful for the detection chip 10 and the detection device. Used in conjunction with.
  • the above numerical range is also helpful for the processing and preparation of the detection chip 10, and is convenient for the user to grasp with one hand, fix or pick up with equipment, and so on.
  • the diameter of the first substrate 101 and the second substrate 102 is 3.5 cm
  • the thickness of the first substrate 101 is 1.5 mm
  • the thickness of the second substrate 102 is 1 mm. Therefore, while the detection chip 10 is designed to be thin and convenient for users, such as one-handed grasping, fixing or picking with equipment, the preparation process of the detection chip 10 can be simplified, and the processing technology and accuracy requirements of the detection chip 10 can be reduced. , Thereby facilitating the processing and preparation of the detection chip 10.
  • the detection chip 10 may further include a sample application protrusion 190.
  • the sample application protrusion 190 protrudes from the first surface of the first substrate 101 in a direction away from the second substrate 102.
  • One end of the sample application protrusion 190 is connected to the sample application opening 110, and the other end can communicate with the atmosphere.
  • the sample addition protrusion 190 can serve the functions of sample containment and flow diversion, so that the sample quickly flows to the sample addition opening 110 through the sample addition protrusion 190, thereby facilitating the injection of the sample into the sample addition opening 110 and increasing the detection chip 10
  • the sample volume is convenient for taking and placing the detection chip 10, for example.
  • the sample application protrusion 190 includes a conical cavity and allows the sample application opening 110 to communicate with the atmosphere.
  • the cone angle of the conical cavity (for example, the angle between the center line of the cone and the side wall) may be 30°-75°, and the volume may be 50 ⁇ L-200 ⁇ L.
  • the conical cavity of the sample application protrusion 190 includes a first end and a second end opposite to each other. The first end is connected to the sample application opening 110 and has a diameter of 0.5mm-5mm, and the second end has a diameter of 1mm- 20mm.
  • the height of the conical cavity of the sample application protrusion 190 protruding from the first substrate 101 is 1 mm-20 mm.
  • the sample addition protrusion 190 can occupy a reasonable space to match the size of the detection device, thereby facilitating the use of the detection chip 10 and the detection device in cooperation.
  • the sample addition protrusion 190 determined by the above numerical range can better serve the functions of sample receiving and diversion, for example, allowing the sample to flow uniformly and at a reasonable speed to the sample addition opening 110, thereby facilitating the addition of The sample is injected into the sample opening 110.
  • the diameter of one end of the sample application protrusion 190 that is connected to the sample application opening 110 is 3 mm
  • the diameter of the other end is 10 mm
  • the height of the sample application protrusion 190 protruding from the first substrate 101 is 1.5 mm. .
  • the sample can quickly flow to the sample adding opening 110 through the sample adding protrusion 190, and it is also helpful for the picking and placement of the detection chip 10 and the use of, for example, the detection device.
  • the preparation process of the sample addition protrusion 190 can also be simplified, thereby reducing the processing technology and accuracy requirements.
  • the sampling opening 110 includes a first body 111 and a first protrusion 112 protruding from the first body 111 to the flow guide groove 130, and the first protrusion 112 is connected to The diversion groove 130 is connected to facilitate the rapid flow of the sample into the diversion groove 130 through the first protrusion 112.
  • the diameter of the first body 111 may be set to 1 mm-10 mm, such as 5 mm or 7 mm. Therefore, it is possible to ensure that the sample can flow into the diversion groove 130 evenly and quickly, and the space required by the detection groove 141 can also be ensured.
  • the shape of the first body 111 may be a circle, a square or other suitable shapes, and the first protrusion 112 may be a square, a sharp angle, or other shapes, which is not limited in the embodiment of the present disclosure.
  • the diagonal length of the first body 111 may be set to be 1 mm-10 mm, such as 5 mm or 7 mm.
  • the circular or square first body 111 can simplify the manufacturing process of the detection chip 10, reduce the processing technology and accuracy requirements of the detection chip 10, and thereby reduce the manufacturing cost of the detection chip 10.
  • the inner wall of the diversion groove 130 may be set to be hydrophilic, for example, the contact angle of the liquid on the inner wall of the diversion groove 130 is less than 90°, which helps the sample to quickly enter the diversion groove 130 and make the sample It can quickly flow into the detection groove 141 through the diversion groove 130 to mix with the reaction film 150, thereby shortening the detection time of the sample and improving the accuracy of the detection data obtained.
  • the hydrophilic treatment agent can be injected into the diversion tank 130, so that the diversion tank 130 is soaked in the hydrophilic treatment agent for 1 minute, and then the hydrophilic treatment agent is discharged with air to make the diversion tank 130
  • the inner wall of the groove 130 may be set to have hydrophilicity.
  • the contact angle of the liquid on the inner wall of the diversion groove 130 refers to the angle formed by the tangent line along the surface of the liquid drop and the inner wall surface of the diversion groove 130.
  • the contact angle of the liquid on the inner wall of the diversion groove 130 is less than 90°, it can be considered that the inner wall of the diversion groove 130 is hydrophilic.
  • the smaller the value of the contact angle of the liquid on the inner wall of the guide groove 130 the better the wettability of the inner wall of the guide groove 130.
  • the contact angle of the liquid on the inner wall of the guide groove 130 is 0°, The inner wall material of the guide groove 130 is completely wetted.
  • the embodiment of the present disclosure does not limit the specific value of the contact angle of the liquid on the inner wall of the diversion groove 130, as long as the contact angle is less than 90° to make the inner wall of the diversion groove 130 hydrophilic.
  • the height of the diversion groove 130 can be set to 0.1mm-1.5mm, such as 0.5mm; the width of the diversion groove 130 can be set to be 0.1mm-2mm, such as 0.5mm. Therefore, it is helpful to control the amount of sample flowing into the detection groove 141 through the flow guide groove 130, and reduce or avoid the waste of the sample in the flow process.
  • the ratio of the height of the flow guide groove 130 to the width of the flow guide groove 130 may be set to 1:1-10:1, such as 2:1. Therefore, in some embodiments, when the first substrate 101 and the second substrate 102 are bonded by a bonding method, for example, the first surface of the first substrate 101 and the second substrate 102 are bonded by an adhesive.
  • the adhesive can be made of a hydrophobic material.
  • the ratio of the width of the groove 130 can reduce the contact area of the hydrophobic adhesive on the bonding surface of the second substrate 102 when the sample flows through the diversion groove 130, thereby realizing the rapid flow of the sample in the diversion groove 130 .
  • the height of the diversion groove 130 is 1 mm, and the width of the diversion groove 130 is 0.5 mm. Therefore, it is helpful to realize the rapid flow of the sample in the diversion groove 130, and can reduce or avoid the waste of the sample in the flow process. At the same time, the preparation process of the diversion groove 130 can be simplified, and the processing technology and accuracy requirements can be reduced. .
  • the second end of the diversion groove 130 (that is, the end communicating with the detection groove 141) is in contact with the reaction film 150, thereby facilitating the immersion of the sample into the reaction film 150, so that the sample It fully reacts with the detection reagent in the reaction film 150.
  • a part of the reaction film 150 may be located in the flow guide groove 130.
  • the reaction film 150 may not be in contact with the second end of the diversion groove 130, which is not limited in the embodiment of the present disclosure.
  • the structure of the detection chip 10 such as the sampling protrusion 190, the sampling opening 110, the diversion groove 130, and the detection groove 141, can be integrally formed by, for example, an injection molding process, thereby simplifying the detection chip. 10's preparation process.
  • the reaction membrane 150 includes a matrix material and detection reagents distributed in the matrix material.
  • the matrix material may include glass fiber, cotton fiber, or a composite fiber of glass fiber and cotton fiber, and the like.
  • the reaction film 150 in a compressed state compressed by the two substrates in the thickness direction.
  • the thickness of the reaction film 150 in the relaxed state may be equal to or slightly higher than the height of the detection groove 141, for example, the ratio of the thickness of the reaction film 150 in the relaxed state to the height of the detection groove 141 may be 1:1-1 : 0.5, and then after the first substrate 101 and the second substrate 102 are combined, since the reactive film 150 is compressed in the thickness direction, the surface of the reactive film 150 facing the second substrate 102 and the first surface of the first substrate 101 It can be located on the same horizontal surface, so that the reaction film 150 and the second substrate 102 are in close contact, which can avoid the gap between the reaction film 150 and the first substrate 101 or the second substrate 102, which may cause liquid retention. As a result, the incoming sample can be more fully immersed in the reaction membrane 150 and reacted more fully with the detection reagent in
  • the compression amount of the reaction film 150 can be set to 10%-40%, so as to help bring the reaction film 150 into close contact with the second substrate 102, thereby weakening or avoiding the reaction film 150 and the second substrate 102.
  • a gap is generated between the first substrate 101 or the second substrate 102 to cause liquid retention.
  • the shape of the reaction film 150 is generally the same as or similar to the shape of the detection groove 141, so that the reaction film 150 is contained in the detection groove 141, and the reaction film 150 and the sample are uniformly mixed.
  • FIG. 6 shows an exemplary structure of the reaction film of the detection chip shown in FIGS. 1A and 1B.
  • the reaction film 150 includes a film main body 151 and a second protrusion 152.
  • the film main body 151 has the same shape as the detection groove 141, and both are circular.
  • the second protruding part 152 protrudes from the film main body 151 to the guide groove 130, and at least part of the second protruding part 152 is located in the guide groove 130, thereby helping to introduce the sample from the guide groove 130 to the detection groove 141, and the sample can be fully immersed in the reaction film 150, so that the sample can fully react with the detection reagent in the reaction film 150, and the accuracy of the obtained detection data is improved.
  • the diameter of the detection groove 141 may be set to 3 mm-15 mm, and the diameter of the film main body 151 of the reaction film 150 is equal to or smaller than the diameter of the detection groove 141.
  • the reaction film 150 can be better contained in the detection groove 141, thereby helping to mix the reaction film 150 and the sample uniformly.
  • the entire reaction film may also be set to the same shape as the detection groove, that is, the reaction film may only have the main body part of the film without the protruding part (for example, the second protruding part). 152), the embodiment of the present disclosure does not limit this.
  • the reaction membrane may also be configured as a polygon, and one corner of the polygon is directly connected with the second end of the diversion groove.
  • the detection groove is a polygon that is the same as or similar to the shape of the reaction film.
  • the reaction film may be set to a regular shape such as a triangle, a square, a rhombus, etc., or may be set to an irregular shape, etc., which is not limited in the embodiments of the present disclosure.
  • FIGS. 7A and 7B are respectively a front perspective view and a back perspective view of another detection chip provided by at least one embodiment of the present disclosure.
  • Fig. 7A is a top view of the detection chip
  • Fig. 7B is a bottom view of the detection chip.
  • Fig. 8 is an exploded view, that is, an exploded view of the detection chip shown in Figs. 7A and 7B.
  • the detection chip 20 includes a sample addition opening 210 and a plurality of detection branch structures 220, and the plurality of detection branch structures 220 are evenly distributed along the periphery of the sample addition opening 210.
  • Each of the plurality of detection branch structures 220 includes a diversion groove 230 and a detection part 240.
  • the diversion groove 230 has a first end and a second end, and the first end is in communication with the sample injection opening 210.
  • the detection part 240 includes a detection groove 241 and a reaction reagent (for example, a reaction membrane 250), the detection groove 241 is in communication with the second end of the diversion groove 230, the reaction membrane 250 is accommodated in the detection groove 241, and the detection part 240 is configured as Optical detection of the reaction film 250 located in the detection groove 241 is allowed.
  • a reaction reagent for example, a reaction membrane 250
  • the reaction film 250 of the detection chip 20 is set to a polygonal shape, such as a square or a rhombus. Accordingly, the shape of the detection groove 241 is also set to a square or a rhombus.
  • the side length of the detection groove 241 may be set to 3 mm-15 mm, such as 5 mm or 10 mm, and the side length of the reaction film 250 is equal to or less than the side length of the detection groove 241.
  • the reaction film 250 can be better contained in the detection groove 241, which helps to mix the reaction film 250 and the sample uniformly.
  • the diagonal of the square may be set to 1mm-20mm, such as 10mm or 15mm.
  • the two diagonal lines of the rhombus can be set to 1mm-20mm respectively; for example, the length of one diagonal line is 10mm, and the length of the other diagonal line is 6mm.
  • the length ratio between the two diagonals of the rhombus can be set to 1:1 to 1:2.
  • the angle between two adjacent sides of the rhombus can be set to be less than or equal to 60°, so as to help the sample to fully immerse into the reaction film 250 along the periphery of the reaction film 250, so that the sample can interact with the reaction film 250.
  • the detection reagents in the system fully react, thereby improving the accuracy of the detection data obtained.
  • one corner of the reaction film 250 is directly connected with the second end of the diversion groove 230 to introduce the sample from the diversion groove 230 into the detection groove 241, and the sample can be fully immersed in the reaction film 250, so that the sample It fully reacts with the detection reagent in the reaction membrane 250 to improve the accuracy of the detection data obtained.
  • FIGS. 9A and 9B are respectively a front perspective view and a back perspective view of the first substrate of the detection chip shown in FIGS. 7A and 7B.
  • FIG. 9A is a top view of the first substrate 201
  • FIG. 9B is a bottom view of the first substrate 201.
  • 10 is a schematic plan view of the first surface of the first substrate of the detection chip shown in FIGS. 7A and 7B.
  • the detection chip 20 further includes a first substrate 201 and a second substrate 202.
  • the first substrate 201 has a first surface and a second surface opposite to each other.
  • the sample application opening 210 is a through hole in the first substrate 201, and the diversion groove 230 and the detection groove 241 are formed on the first surface of the first substrate 201.
  • the second substrate 202 is laminated on the first surface of the first substrate 201 and allows optical detection at a position corresponding to the detection groove 241.
  • the second substrate 202 has a detection through hole 260 at a position corresponding to the detection groove 241, and the reaction film 250 in the detection groove 241 can be optically detected through the detection through hole 260 to obtain different detection indicators of the sample.
  • the shape of the detection through hole 260 is circular, while in some other embodiments of the present disclosure, the shape of the detection through hole 260 may also be set to be the same as the shape of the detection groove 241, for example. Square, rhombus or other shapes are not limited in the embodiments of the present disclosure.
  • the sample addition opening 210 includes a first body 211 and a first protrusion 212 protruding from the first body 211 to the guide groove 230, and the first protrusion 212 is in communication with the guide groove 230. So that the sample flows into the diversion groove 230 through the first protrusion 212.
  • first substrate 201 and the second substrate 202 can refer to the corresponding descriptions of the first substrate 101 and the second substrate 102 of the detection chip 10 in the above-mentioned embodiment, which will not be repeated here. .
  • specific structural parameters, materials, and functions of the detection chip 20 reference may be made to the corresponding description of the detection chip 10 in the foregoing embodiment, and details are not described herein again.
  • the detection portion of the detection chip may further include a diversion groove located at the periphery of the detection groove, for example, the diversion groove may at least partially surround the detection groove.
  • the diversion groove is formed on the first surface of the first substrate and communicates with the detection groove, and the height of at least part of the diversion groove is smaller than the height of the detection groove.
  • the diversion groove surrounds the circumference of the detection groove, that is, around all the peripheral areas of the detection groove, so that the sample can infiltrate more quickly along the periphery of the reaction film through the diversion groove. Then infiltrate from the periphery of the reaction film to the center of the reaction film, thereby further accelerating the mixing speed between the sample and the reaction film, and significantly shortening the required detection time.
  • the amount of sample infiltrated to the center of the reaction film increases due to capillary action, which makes the detection result (such as color development) of the center of the reaction film the most obvious, facilitating subsequent test results Observation, thereby further improving the accuracy of the detection data obtained.
  • the diversion groove may also cross or partially overlap the detection groove in a direction perpendicular to the first surface of the first substrate. No restrictions.
  • FIG. 11 is an exploded view, that is, an exploded view of yet another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 12 is a schematic diagram of the structure of the first substrate of the detection chip shown in FIG. 11.
  • FIG. 12 is a bottom view of the first substrate 301 of the detection chip 30 shown in FIG. 11. It should be noted that, except for the liquid storage through hole 380 and the diversion groove 342, the structure and function of the detection chip 30 shown in FIG. 11 are basically the same as or similar to those of the detection chip 20 shown in FIGS. 7A-8. No longer.
  • the detection part 340 of the detection chip 30 includes a reaction reagent (for example, a reaction film 350 ), a detection groove 341, and diversion grooves 342 and 343 surrounding the detection groove 341.
  • a reaction reagent for example, a reaction film 350
  • a detection groove 341 for example, a detection groove 341
  • diversion grooves 342 and 343 surrounding the detection groove 341.
  • FIG. 13A is a schematic diagram of a partial structure of the first substrate of the detection chip shown in FIG. 12, for example, a schematic diagram of a partial structure of the first substrate 301 of the detection chip 30 including the detection portion 340, and FIG.
  • the cross-sectional structure diagram of the'line, FIG. 13C is the cross-sectional structure diagram along the line B-B' in FIG. 13A. It should be noted that, for the sake of brevity and clarity, the reaction film 350 of the detection part 340 is not shown in FIGS. 12-13C.
  • the diversion grooves 342 and 343 are formed on the first surface 3011 of the first substrate 301 and communicate with the detection groove 341.
  • one end of the diversion groove 342 is connected to the diversion groove 341.
  • 330 is connected, and the other end is connected with the diversion groove 343.
  • the sample when the sample flows into the detection part 340 through the flow guide groove 330, the sample first flows into the flow guide grooves 342 and 343 and rapidly infiltrates the circumference of the reaction film 350 through the flow guide grooves 342 and 343, and then from the reaction
  • the circumference of the film 350 is infiltrated toward the center of the reaction film 350, thereby speeding up the mixing speed between the sample and the reaction film 350, and shortening the required detection time.
  • the amount of sample infiltrated into the center of the reaction film 350 is increased, thereby making the detection result (such as color development) of the center of the reaction film 350 more obvious, facilitating the observation of subsequent test results, thereby improving the obtained Check the accuracy of the data.
  • the longitudinal sections of the diversion groove 342 and the detection groove 341 are stepped as a whole, so that a larger sample supply can be formed between the diversion groove 342 and the reaction film 350.
  • the flow gap thereby further increasing the flow velocity of the sample in the diversion groove 342, so that the sample can quickly infiltrate along the periphery of the reaction film 350, and then infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350, thereby improving
  • the wetting effect of the center of the reaction film 350 makes the detection result (for example, color development) of the center of the reaction film 350 more obvious.
  • the height H2 of the guide groove 342 is smaller than the height H1 of the detection groove 341.
  • the height H1 of the detection groove 341 can be set to 0.2mm-5mm, such as 2mm or 3mm, etc., so that in the state where the first substrate 301 and the second substrate 302 are combined, the reaction film 350 is doubled in the thickness direction.
  • the surface of the reaction film 350 facing the second substrate 302 and the first surface 3011 of the first substrate 301 can be on the same horizontal plane, so that the reaction film 350 is in close contact with the second substrate 302.
  • the difference between the height H2 of the diversion groove 342 and the height H1 of the detection groove 341 is 0.1mm-1mm, such as 0.5mm or 0.8mm, etc., so that the sample can flow quickly in the detection groove 341, so that the sample Wetting along the periphery of the reaction film 350 more quickly.
  • the width D1 of the diversion groove 342 can be set to 0.1 mm-1 mm, such as 0.5 mm or 0.7 mm.
  • a sufficient and appropriate gap for the sample to flow is formed between the diversion groove 342 and the reaction film 350, which in turn helps the sample to infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350, and improves the performance of the reaction film 350.
  • the infiltration effect of the center makes the detection result (for example, color development) of the center of the reaction film 350 more obvious.
  • the diagonal of the square may be set to 1mm-20mm, such as 10mm or 15mm.
  • the two diagonal lines of the rhombus can be set to 1mm-20mm respectively; for example, the length of one diagonal line is 10mm, and the length of the other diagonal line is 6mm.
  • the length ratio between the two diagonals of the rhombus can be set to 1:1 to 1:2.
  • the angle between two adjacent sides of the rhombus can be set to be less than or equal to 60°, which helps the sample to infiltrate the center of the reaction film 350 along the circumference of the reaction film 250, so that the sample can be fully immersed in the reaction film. In the film 250, the accuracy of the obtained detection data is further improved.
  • the diversion groove 342 may also be designed in a stepped shape including a multilayer step structure, which is not limited in the embodiments of the present disclosure.
  • the diversion groove 343 is arranged in a slope-like structure.
  • the diversion groove 343 includes a ramp-shaped diversion wall 344, which is inclined with respect to the first surface 3011 of the first substrate 301, and the first end 3441 of the diversion wall 344 is connected to the first end 3441 of the first substrate 301.
  • a surface 3011 is connected, and the second end 3442 of the guide wall 344 is connected with the side surface of the detecting groove 341. Since the gap formed between the diversion groove 343 and the reaction film 350 for sample flow is smaller than that of the diversion groove 342, the flow velocity of the sample in the diversion groove 343 is slightly lower than that of the sample in the diversion groove 343.
  • the speed of the center infiltration increases the amount of sample infiltrated into the center of the reaction film 350, which in turn makes the detection result (such as color development) of the center of the reaction film 350 more obvious, which is more conducive to the observation of subsequent detection results.
  • the diversion groove 343 can also be set to other suitable structures, as long as the space for the sample flow formed between the diversion groove 343 and the reaction film 350 is satisfied. It is less than or equal to the space formed between the diversion groove 342 and the reaction film 350 for the sample to flow, which is not limited in the embodiment of the present disclosure.
  • the second end 3442 of the flow guide wall 344 is not parallel to the first surface 3011 of the first substrate 301, for example, the second end 3442 of the flow guide wall 344 is opposite to the first surface 3011.
  • the first surface 3011 of the substrate 301 is inclined and extends toward the first surface 3011 of the first substrate 301 along the flow direction of the sample (for example, in the direction away from the sample application opening 310).
  • the surface area of the guide wall 344 (that is, the contact area between the guide wall 344 and the sample) gradually decreases along the flow direction of the sample, for example, gradually decreases in the direction away from the sample injection opening 310, so that The space for the sample to flow formed between the diversion groove 343 and the reaction film 350 gradually decreases along the flow direction of the sample.
  • the speed of the sample infiltration from the periphery of the reaction film 350 to the center of the reaction film 350 is further increased, and the amount of the sample infiltrated into the center of the reaction film 350 is further increased to a certain extent, thereby enabling the detection of the center of the reaction film 350
  • the result (such as color development) is more obvious, which is more conducive to the observation of subsequent detection results, thereby improving the accuracy of the obtained detection data.
  • the diversion wall 344 can also be set to other suitable contour shapes, as long as the contact area between the diversion wall 344 and the sample is gradually reduced along the flow direction of the sample. Small is enough, and the embodiments of the present disclosure do not limit this.
  • the maximum height H4 of the diversion groove 343 is smaller than the height H1 of the detection groove 341, that is, the second end of the diversion wall 344 that meets the side surface of the detection groove 341 is between the first surface 3011 of the first substrate 301 The distance between the two is smaller than the height H1 of the detection groove 341.
  • a gap for sample flow can be formed between the diversion groove 343 and the reaction film 350, so that the sample can quickly infiltrate along the periphery of the reaction film 350, and then infiltrate from the periphery of the reaction film 350 to the center of the reaction film 350. Therefore, the wetting effect of the center of the reaction film 350 is improved, and the detection result (for example, color development) of the center of the reaction film 350 is more obvious.
  • the difference between the distance between the second end of the flow guide wall 344 and the first surface 3011 of the first substrate 301 and the height of the detection groove 341 (that is, the difference between H4 and H1) can be set to 0.1mm-1mm .
  • an appropriate gap for the sample to flow can be formed between the diversion groove 343 and the reaction film 350, thereby improving the wetting effect of the center of the reaction film 350.
  • the width D2 of the diversion groove 343 can be set to 0.1mm-1mm, such as 0.5mm or 0.7mm, etc., so that an appropriate gap for sample flow is formed between the diversion groove 343 and the reaction film 350, thereby The wetting effect of the center of the reaction film 350 is improved.
  • the width D1 of the diversion groove 342 is the same as the width D2 of the diversion groove 343, and the height H2 of the diversion groove 342 is the same as the width D2 of the diversion groove 343.
  • the maximum height H4 of the groove 343 is the same; and in some other embodiments of the present disclosure, the width D1 of the diversion groove 342 and the width D2 of the diversion groove 343 may also be different from each other, and the height H2 of the diversion groove 342 is equal to The maximum height H4 of the guide groove 343 may also be different from each other, which is not limited in the embodiment of the present disclosure.
  • the diversion groove surrounding the detection groove 341 includes the diversion groove 342 and the diversion groove 343 which have different structures from each other.
  • the diversion grooves surrounding the detection groove 341 may also all adopt the same structure, for example, they are all set as diversion grooves 342, or all are set as diversion grooves 343.
  • the embodiments of the present disclosure There is no restriction on this.
  • the diversion groove is provided on the periphery of the diamond-shaped detection groove as an example to describe the structure of the diversion groove.
  • the detection grooves are triangles, circles, and other regular or irregular shapes, it is also possible to provide diversion grooves around the corresponding detection grooves, for example, A diversion groove is provided on the periphery of the detection groove 141 of the detection chip 10 shown in FIGS. 1A-2B, which is not limited in the embodiment of the present disclosure.
  • the diversion grooves 342 and 343 surround the detection groove 341, and the contour shape of the diversion grooves 342 and 343 is the same as the contour shape of the detection groove 341, but in some other embodiments of the present disclosure In an example, the contour shape of the diversion grooves 342 and 343 may also be different from the contour shape of the detection groove 341, which is not limited in the embodiment of the present disclosure.
  • the diversion groove may also partially surround the detection groove.
  • the diversion groove may be provided only on the two sides adjacent to the detection groove and the diversion groove. Flow grooves to achieve rapid mixing between the sample and the reaction membrane.
  • the detection results (such as color development) in a certain area or a certain range on the reaction film near the center can be made more obvious, thereby facilitating subsequent observation of the detection results, and improving the accuracy of the obtained detection data.
  • the diversion groove 330 extends into the detection groove 341, and the second end of the diversion groove 330 communicating with the detection groove 341 is located in the diamond-shaped detection groove 341, so that the sample can be
  • the reaction film 350 is more quickly and fully infiltrated, and the required detection time is shortened.
  • the length of the second end of the diversion groove 330 extending into the detecting groove 341 is less than or equal to 1.5 mm, such as 0.5 mm or 1 mm. Therefore, it can help to introduce the sample from the diversion groove 330 into the detection groove 341, so that the sample can be fully immersed in the reaction film 350, so that the sample can fully react with the detection reagent in the reaction film 350, and the obtained Check the accuracy of the data.
  • the reaction film 350 is in a compressed state along the thickness direction (ie, along the direction of the height H1 shown in FIG. 13A or FIG. 13B).
  • the thickness of the reaction film 350 in the relaxed state may be equal to or slightly higher than the height H1 of the detection groove 341, for example, the ratio of the thickness of the reaction film 350 in the relaxed state to the height H1 of the detection groove 341 may be 1:1 -1:0.5, and then after the first substrate 301 and the second substrate 302 are combined, since the reaction film 350 is compressed in the thickness direction, the reaction film 350 is in close contact with the second substrate 302, thereby avoiding the reaction film 350 and the first substrate A gap is generated between the 301 or the second substrate 302 to cause liquid storage.
  • the detection portion 340 may further include a liquid storage through hole 380 that penetrates the first substrate 301 and communicates with the detection groove 341, so that the amount of sample injected through the sample addition opening 310 is too large.
  • the excess sample can be stored in the liquid storage through hole 380, thereby avoiding or reducing liquid leakage caused by excessive sample injection, and the liquid storage through hole 380 can also promote the sample to pass through the diversion grooves 342 and 343.
  • the periphery of the reaction film 350 is infiltrated toward the center, so that the detection result (for example, color development) of the center of the reaction film 350 is more obvious.
  • the diameter of the liquid storage through hole 380 can be set to 0.2mm-5mm, such as 2mm or 3mm, etc., so as to help control the amount of sample, avoid or reduce leakage caused by excessive sample injection, and promote the sample from the reaction membrane.
  • the periphery of 350 is infiltrated toward the center, and the processing technology and accuracy requirements of the liquid storage through hole 380 can also be reduced, thereby simplifying the preparation process.
  • the ratio of the depth H3 of the liquid storage through hole 380 to the height H1 of the detection groove 341 can be set to 0.5:1-10:1, such as 3:1, etc., so as to serve as a sample
  • the role of quantification is conducive to controlling the amount of sample, and the liquid storage through hole 380 can also store more samples to prevent sample overflow.
  • the liquid storage through hole 380 communicates with the center of the detection groove 341, that is, the liquid storage through hole 380 is opened at the center position of the bottom surface of the detection groove 341, and the center of the detection groove 341 is communicated with the external environment such as the atmosphere, which is better This effectively promotes the infiltration of the sample from the periphery to the center of the reaction film 350.
  • negative pressure may be added to the liquid storage through hole 380 during assembly to fix the reaction film 350 in the detection groove 341. Therefore, the sample can more fully react with the detection reagent in the reaction membrane 350, and the accuracy of the obtained detection data is further improved.
  • the shape of the detection groove 341 is a rhombus, and the two diagonals of the rhombus are 8mm and 5.8mm, respectively, the height H1 of the detection groove 341 is 0.5mm, and the height H1 of the diversion groove 342 is 0.5mm.
  • the difference between the height H2 and the height H1 of the detection groove 341 is 0.3 mm
  • the difference between the maximum height H4 of the diversion groove 343 and the height H1 of the detection groove 341 is 0.3 mm
  • the width D1 of the diversion groove 342 is 0.3 mm
  • the width D2 of the diversion groove 343 is 0.3 mm
  • the diameter of the liquid storage through hole 380 is 2.5 mm
  • the depth H3 of the liquid storage through hole 380 is 1 mm.
  • the amount of sample in the detection groove 341 avoid or reduce leakage caused by excessive sample injection, and at the same time promote the infiltration of the sample from the periphery to the center of the reaction film 350, so that the center of the reaction film 350 is infiltrated.
  • the amount of the sample is increased, so that the detection result (for example, color development) of the center of the reaction film 350 is more obvious, which is more conducive to the observation of the subsequent detection result.
  • the liquid storage through hole may also be opened in other positions on the bottom surface of the detection groove; or, according to actual needs, multiple liquid storage channels may also be opened in each detection part. hole.
  • the embodiments of the present disclosure do not limit the location and number of the liquid storage through holes.
  • FIG. 14 is an exploded view, that is, an exploded view of yet another detection chip provided by at least one embodiment of the present disclosure.
  • Fig. 14 is a top view of the detection chip, showing the structure viewed from the front of the detection chip. It should be noted that, except for the adhesive layer 704 and the sample application protrusion 790, the structure and function of the detection chip 70 shown in FIG. 14 are basically the same as or similar to those of the detection chip 30 shown in FIG. 11, and will not be repeated here. .
  • FIG. 14 shows a situation where the detection chip 70 combines the first substrate 701 and the second substrate 702 through an adhesive layer 704.
  • the adhesive layer 704 is laminated between the first substrate 701 and the second substrate 702, that is, is laminated on the first surface of the first substrate 701.
  • the adhesive layer 704 can be made of, for example, a hydrophobic material such as double-sided tape.
  • the adhesive layer 704 includes openings 7041 corresponding to the detection grooves and diversion grooves (not shown) of the first substrate 701, thereby avoiding uneven surface of the adhesive layer 704. It also hinders the flow of the sample due to its own viscosity. At the same time, it can also avoid the adhesion between the adhesive layer 704 and the reaction film 750, which helps the sample flow in the detection groove and the diversion groove, so that the sample The reaction film 750 can be quickly and fully infiltrated, and the accuracy of the obtained detection result can be improved.
  • the shape and size of the opening 7041 may be the same as the shape and size of the cross-section of the detection groove, thereby reducing or avoiding the obstruction of the adhesive layer 704 to the flow of the sample, or reducing or avoiding The bonding layer 704 and the reaction film 750 are bonded.
  • the shape of the opening 7041 may also be the same as the shape of the cross section of the detection groove, and the size of the opening 7041 is slightly smaller than the cross section of the detection groove, thereby slightly increasing the adhesive layer 704 The contact area with the first substrate 701 and the second substrate 702 to improve the adhesion between the first substrate 701 and the second substrate 702, thereby further reducing or avoiding sample leakage, and improving the sealing effect of the detection chip 70 .
  • the tip portion (for example, the top corner portion) of the opening 7041 may be set to a right angle, or may be set to a rounded corner with a certain arc, for example, it may be a right angle type or a circular arc type.
  • the four corners of the rhombus may be set at right angles or may be set at rounded corners with a certain arc, which is not limited in the embodiment of the present disclosure.
  • the two diagonal groups of the diamond can be divided into a first group and a second group, and the first group of diagonal corners are set to right angles, and the second group
  • the diagonal is set in a circular arc shape.
  • the shape of the diamond-shaped opening 7041 is basically the same as the contour shape of the detection groove of the first substrate 701, the size of the diamond-shaped opening 7041 can be slightly smaller than the cross-section of the detection groove by making the first set of diagonal corners arc-shaped.
  • the contact area between the bonding layer 704 and the first substrate 701 and the second substrate 702 is increased to improve the bonding effect between the first substrate 701 and the second substrate 702;
  • the second set of diagonals is set at a right angle, which can reduce or avoid the obstruction of the adhesive layer 704 to the flow of the sample to a certain extent, and can also reduce or prevent the adhesion between the adhesive layer 704 and the reaction film 750.
  • the first set of diagonal corners and the second set of diagonal corners it is possible to reduce or avoid problems such as the adhesion layer 704 hindering the flow of the sample due to its own viscosity and the occurrence of adhesion with the reaction film 750. It is also possible to better realize the combination between the first substrate 701 and the second substrate 702, reduce or avoid sample leakage, and improve the sealing effect of the detection chip 70.
  • all the top corners of the opening 7041 may be designed to be right angles, or all to be designed to be arc-shaped or other suitable contours, which are not limited in the embodiments of the present disclosure.
  • the shape and size of the opening 7041 are basically the same as the contour shape and size of the detection groove and the diversion groove of the first substrate 701, but in some other aspects of the present disclosure
  • the size of the opening of the adhesive layer can also be slightly larger or slightly smaller than the size of the detection groove or the diversion groove, or it can be set to other suitable shapes or sizes, as long as it can avoid or weaken the adhesion of the adhesive layer.
  • the sample infiltration reaction film is obstructed and the sealing effect of the detection chip can be ensured, which is not limited in the embodiment of the present disclosure.
  • the contact area between the first substrate 701 is helpful for the stable connection between the sample application protrusion 790 and the first substrate 701, and is more suitable for the user to hold with one hand, fix or pick up with equipment, and so on.
  • the first substrate 701 includes a first notch 7011
  • the second substrate 702 includes a second notch 7021 corresponding to the first notch 7011, which is perpendicular to the first substrate 701 or the second substrate 702.
  • the first notch 7011 and the second notch 7021 form a limit part (or called a limit block), so that the detection chip 70 can be fixed to the limit block formed by the first notch 7011 and the second notch 7021
  • the positions of the first notch 7011 and the second notch 7021 can also be used to label and locate multiple detection branch structures in the detection chip 70, thereby facilitating the observation and recording of the detection data, for example.
  • the size of the first recess 7011 and the second recess 7021 can be determined according to the structure of the detection device that needs to be fixed with it.
  • One recess 7011 and the second recess 7021 can be fixed on the detection device.
  • the first recess 7011 and the second recess 7021 may be arranged between adjacent detection grooves, or other suitable positions. The embodiment does not limit this.
  • the structural layer or functional layer when the detection chip 70 includes an adhesive layer 704 or other structural layers or functional layers, the structural layer or functional layer also has a notch structure at positions corresponding to the first notch 7011 and the second notch 7021, So that the detection chip 70 forms a limit block as a whole.
  • the structure of the sample opening, diversion groove, and detection groove of the detection chip can also be arranged in other ways, as long as the structure of the sample opening, diversion groove, and detection groove can be realized. Layout and realize the corresponding functions.
  • the above embodiments are introduced in the case where the sample application opening penetrates the first substrate and the diversion groove, the detection groove and other structures are provided on the first surface of the first substrate of the detection chip, but this does not constitute a limitation of the present disclosure.
  • FIGS. 15A and 15B are exploded views, that is, exploded views, of yet another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 15A is a top view of the detection chip
  • FIG. 15B is a bottom view of the detection chip.
  • the structure and function of specific components in the detection chip 40 shown in FIG. 15A and FIG. 15B can refer to the corresponding description of the detection chip 10, the detection chip 20, or the detection chip 30 in the foregoing embodiment, and will not be omitted here. Go into details.
  • the detection chip 40 includes a first substrate 401, a second substrate 402, and a third substrate 403.
  • the first substrate 401 has a first surface (shown as an upper surface in the figure) and a second surface (shown as a lower surface in the figure) opposite to each other, and the diversion groove 430 is formed on the second surface of the first substrate 401,
  • the detection groove 441 is formed on the first surface of the first substrate 401.
  • the second substrate 402 is laminated on the first surface of the first substrate 401 and has a detection through hole 460 at a position corresponding to the detection groove 441 to allow optical detection.
  • the third substrate 403 is laminated on the second surface of the first substrate 401 to seal the diversion groove 430 and the sample addition opening 410 so that the sample can flow into the diversion groove 430 from the sample addition opening 410.
  • the sample application opening 410 is a through hole penetrating the first substrate 401
  • the second substrate 402 has a through hole exposing the sample application opening 410, so that the sample can be injected into the sample application opening 410, and the sample application protrusion 490 can be It extends from the second substrate 402 to facilitate the pickup and placement of the detection chip 40, for example.
  • FIG. 16 is an exploded view, that is, an exploded view of yet another detection chip provided by at least one embodiment of the present disclosure.
  • FIG. 16 is a top view of the detection chip. It should be noted that the structure and functions of specific components in the detection chip 50 shown in FIG. 16 can refer to the corresponding descriptions of the detection chip 10, the detection chip 20, or the detection chip 30 in the foregoing embodiment, and details are not described herein again.
  • the detection chip 50 includes a first substrate 501 and a second substrate 502.
  • the first substrate 501 has a first surface (shown as an upper surface in the figure) and a second surface (shown as a lower surface in the figure) opposite to each other, and the guide groove 530 and the detection groove 541 are formed on the first substrate 501.
  • the second substrate 502 is laminated on the first surface of the first substrate 501 and has a detection through hole 560 at a position corresponding to the detection groove 541 to allow optical detection.
  • the sample application opening 510 is an opening in the first substrate 501.
  • the opening is, for example, a non-through hole that does not penetrate the first substrate 501.
  • the height (or depth) of the sample application opening 510 is smaller than that of the first substrate 501. thickness of.
  • the second substrate 502 has a through hole exposing the sample application opening 510 and a sample application protrusion 590, so that the sample can be injected into the sample application opening 510 through the sample application protrusion 590, and facilitates the handling and placement of the detection chip 50, for example .
  • At least one embodiment of the present disclosure also provides a detection system.
  • the detection system includes: a detection device and a detection chip provided in any embodiment of the present disclosure, such as the detection chip 10, the detection chip 20, and the detection chip 30 in the foregoing embodiment.
  • the detection chip 40 or the detection chip 50, the detection device is configured to detect the reaction film in the detection groove through the detection part of the detection chip.
  • FIG. 17 is a schematic diagram of a detection system provided by at least one embodiment of the present disclosure.
  • the detection system 60 includes a detection chip 10 and a detection device 610.
  • the detection device 610 is configured to detect the reaction reagent, such as the reaction film 150, in the detection groove 141 through the detection portion 140 of the detection chip 10 .
  • the detection device 610 includes a light source 611 and a photodetection device 612.
  • the light source 611 is configured to emit light to the reaction film 150
  • the photodetection device 612 is configured to receive light emitted from the light source 611 and reflected by the reaction film 150.
  • the photodetection device 612 can compare the intensity of the light reflected by the reaction film 150 with the intensity of the light emitted by the light source 611, so as to determine the presence or absence of the test object in the sample according to the value of the detected absorbance of the reaction film 150 Concentration, etc., to achieve the detection of sample indicators. For example, taking the pre-titrated detection reagent on the reaction film 150 as a color reagent as an example, when the detection result is obtained, the darker the color appears on the reaction film 150, the higher the content of the analyte in the test sample, and the corresponding The value of the absorbance of the reaction film 150 detected by the photodetection device 612 is also larger.
  • the photodetection device 612 may be a photodiode.
  • the photodiode can convert the received optical signal into an electrical signal, and then can determine the reception based on the change of the electrical parameter in the electrical signal (such as the change of current, etc.).
  • the intensity of the light to determine the value of the absorbance of the reaction film 150.
  • the corresponding content in the detection chip provided by the embodiments of the present disclosure.
  • the detection chip 10 detection chip 20
  • detection chip 30 detection The corresponding content of the chip 40 or the detection chip 50 will not be repeated here.

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Abstract

一种检测芯片（10）和检测系统（60），检测芯片（10）包括加样开口（110）和至少一个检测分支结构（120）；至少一个检测分支结构（120）中的每个包括检测部（140）；检测部（140）包括检测凹槽（141）和反应试剂（150），检测凹槽（141）与加样开口（110）连通，反应试剂（150）容纳在检测凹槽（141）中，检测部（140）被配置为允许对位于检测凹槽（141）中的反应试剂（150）进行光学检测。该检测芯片（10）可有助于实现自动化检测，并可有助于实现便于携带的检测装置。

Description

检测芯片及检测系统
本申请要求于2020年4月30日递交的中国专利申请第202010367824.8号的优先权，该中国专利申请的全文以引入的方式并入以作为本申请的一部分。
技术领域
本公开的实施例涉及一种检测芯片及检测系统。
背景技术
微流控芯片技术把生物、化学和医学等领域中所涉及的样品制备、反应、分离、检测等基本操作单元集成到一块具有微米尺度微通道的芯片上，自动完成反应和分析的全过程。该过程所使用的芯片叫做微流控芯片，也可称为芯片实验室(Lab-on-a-chip)。微流控芯片技术具有样品用量少，分析速度快，便于制成便携式仪器，适用于即时、现场分析等优点，已广泛应用于生物、化学和医学等诸多领域。
发明内容
本公开至少一个实施例提供一种检测芯片，该检测芯片包括加样开口和至少一个检测分支结构；所述至少一个检测分支结构中的每个包括检测部，检测部包括检测凹槽和反应试剂，其中，所述检测凹槽与所述加样开口连通，所述反应试剂容纳在所述检测凹槽中，所述检测部被配置为允许对位于所述检测凹槽中的所述反应试剂进行光学检测。
例如，在本公开至少一个实施例提供的检测芯片中，所述至少一个检测分支结构中的每个还包括导流槽，所述导流槽具有第一端和第二端，所述导流槽的第一端与所述加样开口连通，所述导流槽的第二端与所述检测凹槽连通，以使所述检测凹槽通过所述导流槽与所述加样开口连通。
例如，本公开至少一个实施例提供的检测芯片还包括：第一基板，具有第一表面，其中，所述加样开口为所述第一基板中的通孔，所述导流槽和所述检测凹槽形成在所述第一基板的第一表面；第二基板，层叠在所述第一基板的第一表面上且在对应于所述检测凹槽的位置允许进行所述光学检测。
例如，在本公开至少一个实施例提供的检测芯片中，所述至少一个检测分支结构中的每个还包括吸水膜，所述吸水膜容纳在所述检测凹槽中，且与所述反应试剂在垂直于所述第一基板的方向上彼此至少部分层叠。
例如，在本公开至少一个实施例提供的检测芯片中，所述吸水膜设置在 所述反应试剂的远离所述第二基板的一侧。
例如，在本公开至少一个实施例提供的检测芯片中，所述吸水膜的液体存储量为10μL-50μL。
例如，在本公开至少一个实施例提供的检测芯片中，所述检测部还包括位于所述检测凹槽周边的导流凹槽，所述导流凹槽与所述检测凹槽连通；至少部分所述导流凹槽的高度小于所述检测凹槽的高度。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流凹槽包括呈斜坡状的导流壁，所述导流壁的一端与所述检测凹槽的侧表面相接。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流凹槽与所述检测凹槽的纵截面整体呈阶梯状。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流凹槽至少部分围绕所述检测凹槽。
例如，在本公开至少一个实施例提供的检测芯片中，所述检测凹槽的高度为0.2mm-5mm，所述导流凹槽的最大高度与所述检测凹槽的高度的差值为0.1mm-1mm，所述导流凹槽的宽度为0.1mm-1mm。
例如，在本公开至少一个实施例提供的检测芯片中，所述检测部还包括贯穿所述第一基板且与所述检测凹槽连通的储液通孔。
例如，在本公开至少一个实施例提供的检测芯片中，所述储液通孔连通所述检测凹槽的中心。
例如，在本公开至少一个实施例提供的检测芯片中，所述储液通孔的直径为0.2mm-5mm，所述储液通孔的深度与所述检测凹槽的高度的比值为0.5:1-10:1。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流槽的高度为0.1mm-1.5mm，所述导流槽的宽度为0.1mm-2mm。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流槽的高度与所述导流槽的宽度的比值为1:1-10:1。
例如，在本公开至少一个实施例提供的检测芯片中，所述导流槽的内壁具有亲水性。
例如，在本公开至少一个实施例提供的检测芯片中，所述至少一个检测分支结构包括多个检测分支结构，所述多个检测分支结构沿所述加样开口的周边均匀分布。
例如，在本公开至少一个实施例提供的检测芯片中，所述加样开口包括第一主体以及从所述第一主体向所述导流槽凸出的第一凸出部，所述第一凸出部与所述导流槽连通。
例如，在本公开至少一个实施例提供的检测芯片中，所述第一主体的直径为1mm-10mm。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应试剂包括反应膜和/或块状反应试剂。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应膜沿厚度方向处于压缩状态。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应膜处于松弛状态时的厚度与所述检测凹槽的高度的比值为1:1-1:0.5。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应膜为圆形，或者所述反应膜为多边形，且所述多边形的一个角与所述导流槽的第二端直接连通。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应膜包括膜主体以及从所述膜主体向所述导流槽凸出的第二凸出部，所述第二凸出部的至少部分位于所述导流槽中；所述膜主体与所述检测凹槽的形状相同，为圆形。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应膜为菱形。
例如，在本公开至少一个实施例提供的检测芯片中，所述检测凹槽的直径为3mm-15mm，所述反应膜的直径等于或小于所述检测凹槽的直径。
例如，在本公开至少一个实施例提供的检测芯片中，所述第二基板在对应于所述检测凹槽的位置具有检测通孔。
例如，在本公开至少一个实施例提供的检测芯片中，所述第二基板不透光；或者，所述检测芯片还包括遮光层，所述遮光层覆盖所述第二基板远离和/或靠近所述第一基板的表面，且暴露所述检测通孔。
例如，在本公开至少一个实施例提供的检测芯片中，所述检测通孔的直径为2mm-10mm。
例如，在本公开至少一个实施例提供的检测芯片中，所述第一基板的材料包括聚甲基丙烯酸甲酯、聚苯乙烯和聚碳酸酯中的一种或多种。
例如，在本公开至少一个实施例提供的检测芯片中，所述第二基板的材料包括聚甲基丙烯酸甲酯、聚苯乙烯、聚碳酸酯和聚对苯二甲酸乙二醇酯中的一种或多种。
例如，在本公开至少一个实施例提供的检测芯片中，所述第一基板与所述第二基板通过键合、焊接、粘结或卡接的方式结合。
例如，本公开至少一个实施例提供的检测芯片还包括粘结层；其中，所述粘结层位于所述第一基板和所述第二基板之间且被用于使所述第一基板和所述第二基板结合，所述粘结层包括对应于所述检测凹槽的开口。
例如，在本公开至少一个实施例提供的检测芯片中，所述反应试剂包括基体材料以及分布在所述基体材料中的检测试剂，所述基体材料包括玻璃纤维、棉纤维或玻璃纤维和棉纤维的复合纤维。
例如，本公开至少一个实施例提供的检测芯片还包括：第一基板，具有第一表面，其中，所述加样开口为所述第一基板中的通孔，所述导流槽和所述检测凹槽形成在所述第一基板的第一表面；第二基板，层叠在所述第一基板的第一表面上，并且在对应于所述检测凹槽的位置具有检测通孔，以允许通过所述检测通孔进行所述光学检测；所述至少一个检测分支结构包括多个检测分支结构，所述多个检测分支结构沿所述加样开口的周边均匀分布。
例如，本公开至少一个实施例提供的检测芯片还包括：第一基板，具有相对的第一表面和第二表面，其中，所述加样开口为所述第一基板中的通孔，所述检测凹槽形成在所述第一基板的第一表面，所述导流槽形成在所述第一基板的第二表面；第二基板，层叠在所述第一基板的第一表面上且在对应于所述检测凹槽的位置允许进行所述光学检测；第三基板，层叠在所述第一基板的第二表面上且密封所述加样开口和所述导流槽。
例如，本公开至少一个实施例提供的检测芯片还包括：第一基板，具有第一表面，其中，所述加样开口、所述导流槽和所述检测凹槽形成在所述第一基板的第一表面，所述加样开口为所述第一基板中的非通孔；第二基板，层叠在所述第一基板的第一表面上且暴露所述加样开口，并在对应于所述检测凹槽的位置允许进行所述光学检测。
例如，本公开至少一个实施例提供的检测芯片还包括光学校准分支结构，其中，所述光学校准分支结构包括光路检测区，所述光路检测区被配置为进行光学校准。
例如，本公开至少一个实施例提供的检测芯片还包括加样凸出部：其中，所述加样凸出部从所述第一基板的第一表面沿远离所述第二基板的方向突出，所述加样凸出部的一端与所述加样开口相接。
例如，在本公开至少一个实施例提供的检测芯片中，所述加样凸出部包括圆锥形腔体，所述圆锥形腔体的容积为50μL-200μL，所述圆锥形腔体突出所述第一基板的高度为1mm-20mm；所述圆锥形腔体包括彼此相对的第一端和第二端，所述第一端与所述加样开口相接，所述第一端的直径为0.5mm-5mm，所述第二端的直径为1mm-20mm。
例如，在本公开至少一个实施例提供的检测芯片中，所述第一基板包括第一凹口，所述第二基板包括与所述第一凹口对应的第二凹口，所述第一凹口和所述第二凹口被用于固定所述检测芯片。
本公开至少一个实施例还提供一种检测系统，包括：检测装置和本公开任一实施例所述的检测芯片；检测装置配置为通过所述检测部对所述检测凹槽中的所述反应试剂进行检测。
例如，在本公开至少一个实施例提供的检测系统中，所述检测装置包括：光源，配置为向所述反应试剂发光；光电检测装置，配置为接收从所述光源 发出且被所述反应试剂反射的光。
附图说明
为了更清楚地说明本公开实施例的技术方案，下面将对实施例的附图作简单地介绍，显而易见地，下面描述中的附图仅仅涉及本公开的一些实施例，而非对本公开的限制。
图1A和图1B分别为本公开至少一实施例提供的一种检测芯片的正面透视图和背面透视图；
图2A和图2B分别为图1A和图1B中所示的检测芯片的爆炸图；
图3A和图3B分别为图1A和图1B所示的检测芯片的第一基板的正面透视图和背面透视图；
图4为图1A和图1B所示的检测芯片的第一基板的第一表面的平面结构示意图；
图5为图1A和图1B所示的检测芯片的第二基板的平面结构示意图；
图6为图1A和图1B所示的检测芯片的反应膜的结构示意图；
图7A和图7B分别为本公开至少一实施例提供的另一种检测芯片的正面透视图和背面透视图；
图8为图7A和图7B中所示的检测芯片的爆炸图；
图9A和图9B分别为图7A和图7B所示的检测芯片的第一基板的正面透视图和背面透视图；
图10为图7A和图7B所示的检测芯片的第一基板的第一表面的平面结构示意图；
图11为本公开至少一实施例提供的再一种检测芯片的爆炸图；
图12为图11所示的检测芯片的第一基板的结构示意图；
图13A为图12所示的检测芯片的第一基板的部分结构示意图；
图13B为图13A中沿A-A’线的截面结构示意图；
图13C为图13A中沿B-B’线的截面结构示意图；
图14为本公开至少一实施例提供的再一种检测芯片的爆炸图；
图15A和图15B为本公开至少一实施例提供的再一种检测芯片的爆炸图；
图16为本公开至少一实施例提供的再一种检测芯片的爆炸图；以及
图17为本公开至少一实施例提供的一种检测系统的示意图。
具体实施方式
为使本公开实施例的目的、技术方案和优点更加清楚，下面将结合本公开实施例的附图，对本公开实施例的技术方案进行清楚、完整地描述。显然， 所描述的实施例是本公开的一部分实施例，而不是全部的实施例。基于所描述的本公开的实施例，本领域普通技术人员在无需创造性劳动的前提下所获得的所有其他实施例，都属于本公开保护的范围。
除非另外定义，本公开使用的技术术语或者科学术语应当为本公开所属领域内具有一般技能的人士所理解的通常意义。本公开中使用的“第一”、“第二”以及类似的词语并不表示任何顺序、数量或者重要性，而只是用来区分不同的组成部分。“包括”或者“包含”等类似的词语意指出现该词前面的元件或者物件涵盖出现在该词后面列举的元件或者物件及其等同，而不排除其他元件或者物件。“连接”或者“相连”等类似的词语并非限定于物理的或者机械的连接，而是可以包括电性的连接，不管是直接的还是间接的。“上”、“下”、“左”、“右”等仅用于表示相对位置关系，当被描述对象的绝对位置改变后，则该相对位置关系也可能相应地改变。
在微流控芯片的设计过程中，发明人希望尽可能多地将分析检测的各项功能集成到芯片上，以减少芯片对外部操作的依赖，从而实现自动化和集成化。例如，可以将微流控芯片的进样部件以及分析检测部件集成在一起，并通过结构的设计实现检测过程的自动化，进而减少对操作人员的技术要求，降低人为误差，使获得的检测数据更加精确。
本公开至少一个实施例提供一种检测芯片，该检测芯片包括加样开口和至少一个检测分支结构。至少一个检测分支结构中的每个包括检测部；检测部包括检测凹槽和反应试剂，检测凹槽与加样开口连通，反应试剂容纳在检测凹槽中，检测部被配置为允许对位于检测凹槽中的反应试剂进行光学检测。
本公开上述至少一个实施例提供的检测芯片可以将用于样品检测的多个基本结构单元或部件集成到同一芯片上，并且通过主动控制或毛细作用完成样品分析检测的全过程，从而实现自动化和集成化的检测过程。由此，采用上述检测芯片可以降低检测过程中可能存在的人为误差，提升检测数据的精确性，同时还可以使检测芯片的整体外形实现薄型化或小型化的设计，有助于实现便携式检测系统。
下面通过几个具体的实施例对本公开提供的检测芯片和检测系统进行说明。
在本公开的一些实施例中，至少一个检测分支结构中的每个还包括导流槽，导流槽具有第一端和第二端，导流槽的第一端与加样开口连通，导流槽的第二端与检测凹槽连通，以使检测凹槽通过导流槽与加样开口连通。
图1A和图1B分别为本公开至少一实施例提供的一种检测芯片的正面透视图和背面透视图，图2A和图2B分别为图1A和图1B中所示的检测芯片的爆炸图，也即分解图。图1A和图2A为检测芯片的俯视图，示出的是从检测芯片正面观看到的结构；图1B和图2B为检测芯片的仰视图，示出的是从 检测芯片背面观看到的结构。
例如，如图1A-图2B所示，检测芯片10包括加样开口110和至少一个检测分支结构120，例如多个检测分支结构120，图中示出六个检测分支结构120作为示例进行介绍。加样开口110用于加入被检测样品，例如母乳、体液、血液等被检测样品。多个检测分支结构120沿加样开口110的周边均匀分布，从而多个检测分支结构120可以分别独立地实现检测功能。
例如，多个检测分支结构120中的每个包括导流槽130和检测部140。导流槽130具有第一端和第二端，第一端与加样开口110连通。检测部140包括检测凹槽141和反应试剂150，检测凹槽141与导流槽130的第二端连通，反应试剂150容纳在检测凹槽141中。检测部140配置为允许对检测凹槽141中的反应试剂150进行光学检测。
例如，在一些实施例中，反应试剂150可以为如图1A-图2B中所示的膜片状，也即反应膜；或者，在一些实施例中，反应试剂150可以为块状或粉末状的反应试剂，例如可以为块状或粉末状的冻干试剂等；或者，在一些实施例中，例如在检测芯片包括多个检测分支结构的情形，也可以是一部分检测凹槽中容纳的反应试剂为膜片状的反应膜，另一部分检测凹槽中容纳的反应试剂为不同于膜片状的反应膜的例如块状或粉末状的反应试剂，本公开的实施例对此不作限制。
下面以反应试剂150为反应膜(即反应膜150)为例，对本公开的实施例提供的检测芯片进行说明。需要说明的是，本公开的实施例包括但并不仅限于此。
例如，在一些实施例中，检测芯片还包括光学校准分支结构，光学校准分支结构包括光路检测区，光路检测区被配置为进行光学校准，由此提升通过光学检测获得的检测数据的准确性和精确性。
例如，在检测芯片包括多个检测分支结构的情况下，光学校准分支结构和多个检测分支结构沿加样开口的周边均匀分布。光学校准分支结构中不设置反应膜，例如，光学校准分支结构除反应膜以外的其他结构可以均与检测分支结构基本相同或相似，由此光学校准分支结构可以与检测分支结构一体设置，从而简化检测芯片的制备工艺和制备成本。
例如，以图1A-图2B所示的检测芯片10为例，可以在一个检测分支结构120中不设置反应膜150而使其作为检测芯片10的光学校准分支结构以用于光学校准，例如可以在图2B所示的一个检测分支结构120中不设置例如反应膜150(以及下文所述的吸水膜170)而使其作为检测芯片10的光学校准分支结构。
例如，光学校准分支结构包括光路检测区，该光路检测区可以为对应于检测凹槽141的位置。由此，在利用检测装置对检测芯片10中的反应膜150 进行光学检测时，可以通过光学校准分支结构对检测装置发出的光路进行校准，例如对光路的照射角度进行校准，从而提升获得的检测数据的准确性。
需要说明的是，在本公开的一些实施例中，根据实际需求，也可以提供两个或更多个光学校准分支结构以用于光学校准。例如，以图1A-图2B所示的检测芯片10为例，可以在两个检测分支结构120中不设置例如反应膜150而使其作为检测芯片10的光学校准分支结构，该两个光学校准分支结构可以相对于加样开口110中心对称，从而提升光学校准的准确度和精确度，进一步提升获得的检测数据的准确性。
需要说明的是，在本公开的其他一些实施例中，根据实际需求，检测芯片也可以采用其他方式实现校准，相应地，校准分支结构也可以是除光学校准以外的其他类型的校准结构，例如在其他类型的校准结构中，可以根据实际需要设置反应膜，或不设置反应膜(或者其他可能对校准产生影响的结构或部件等，例如下文所述的吸水膜170)，本公开的实施例对此不作限制。
例如，反应膜150中包括检测试剂；从加样开口110加入的被检测样品通过导流槽130进入到检测凹槽141中，并与反应膜150中的检测试剂进行反应。此时，可以通过例如光学检测来检测检测试剂和被检测样品发生反应后在反应膜150上体现出来的例如颜色变化等，实现对被检测样品的检测，例如被检测样品中某种成分的有无或者含量的大小等。
由此，本公开的至少一个实施例提供的检测芯片10可以实现例如混合、分析检测等多个功能的集成，并通过主动控制以及毛细作用完成样品分析检测的全过程，从而实现自动化和集成化的检测过程，降低检测过程中可能存在的人为误差，提升检测数据的精确性。并且，在一些实施例中，检测芯片10可以在多个检测分支结构120的检测凹槽141中分别放置包括不同检测试剂的反应膜150，从而可以利用不同检测试剂对同一样品中的多个指标同时进行检测，缩短样品的检测周期，进而实现样品多指标的及时检测。
例如，在一些示例中，被检测样品可以为液体，例如为母乳样品。在检测芯片10的制造过程中，可以在初始的反应膜150上预先滴定所需的检测试剂(例如显色试剂)，待检测试剂浸入反应膜150之后进行烘干，然后再将包括检测试剂的反应膜150置于检测芯片10的检测凹槽中，从而有利于检测芯片10的存储和运输。在利用检测芯片10进行检测时，将样品从加样开口110加入，使样品经导流槽130流入检测凹槽141中并与反应膜150上的检测试剂混合以发生反应。由此，通过对反应结果的检测(例如通过识别反应膜150上颜色的变化)来判断例如样品中待检物的有无以及含量的大小等，从而实现对样品某一指标的检测。
例如，在上述至少一个实施例中，在利用检测芯片10检测样品时，注入的样品量可以为60μL-80μL。由此，可以减少在利用检测芯片10检测样品 时所需的样品量，进而减少或避免样品的浪费。
例如，在一些实施例中，如图1A-图2B所示，每个检测分支结构120还可以包括吸水膜170，吸水膜170容纳在检测凹槽141中，且与反应膜150在反应膜150的厚度方向上(也即在垂直于下文所述的第一基板101的方向上)彼此至少部分层叠，例如二者直接接触。
例如，每个吸水膜170的液体存储量可以为10μL-50μL，例如为30μL。吸水膜170可以容纳一定量的样品，超过吸水膜170的液体存储量后，吸水膜170将不再吸收样品，从而起到样品定量的作用，有利于控制样品量。
例如，吸水膜170的材料可以包括玻璃纤维、棉纤维或玻璃纤维和棉纤维的复合纤维等材料。
需要说明的是，在本公开的其他一些实施例中，检测芯片也可以不采用吸水膜，此时，反应膜150可以同时起到样品定量以及反应载体的作用。
需要说明的是，在本公开的实施例中，多个导流槽130的长度、宽度及深度等彼此相同，由此可以控制流入不同检测凹槽141内的样品量相对保持均匀一致，有利于控制检测凹槽141内的样品量，同时还可以使不同检测凹槽141内样品与反应膜150上的检测试剂之间的反应时间保持相对一致，从而提升获得的检测数据的准确性。
而在本公开的其他一些实施例中，例如在利用不同检测试剂对同一样品中的多个指标同时进行检测的情形，可以根据所需检测的样品的不同指标，使多个导流槽130分别具有不同的尺寸，例如具有不同的长度、宽度或深度等，从而可以控制不同检测凹槽141内的样品量以及不同检测凹槽141内样品与反应膜150上的检测试剂之间的反应时间，由此可以根据实际需求，同时获得多个更加准确的检测数据。
需要说明的是，在本公开的实施例中，导流槽130的具体长度、宽度及深度等可以根据例如样品量、样品特性等实际需求而确定。例如，在实现使样品从加样开口110流入检测凹槽141内的前提下，可以适当减小导流槽130的长度以减少或避免样品在流动过程中的浪费。
在本公开的其他一些实施例中，检测芯片10中还可以不设置导流槽130，例如使检测凹槽141与加样开口110直接相接以实现连通，由此可以进一步减少或避免样品在流动过程中的浪费。此时，在一些示例中，加样开口110中可以具有分别与多个检测凹槽141连通的导流结构，以使加入加样开口110中的样品可以均匀流入多个检测凹槽141中。
例如，在一些实施例中，多个检测凹槽141的尺寸也可以根据所需检测的样品的不同指标、所需的样品的量以及检测试剂的种类等试剂情况进行选择。根据实际需求，多个检测凹槽141的尺寸、形状可以相同也可以不同，本公开的实施例对此不作限制。
例如，在一些实施例中，检测芯片10还包括第一基板101和第二基板102。第一基板101具有相对的第一表面和第二表面，加样开口110为第一基板101中的通孔，导流槽130和检测凹槽141形成在第一基板101的第一表面。第二基板102层叠在第一基板101的第一表面上且在对应于检测凹槽141的位置允许进行光学检测。
例如，图3A和图3B分别为图1A和图1B所示的检测芯片的第一基板的正面透视图和背面透视图。图3A为第一基板101的俯视图，示出的是第一基板的正面结构，图3B为第一基板101的仰视图，示出的是第一基板的背面结构。图4为图1A和图1B所示的检测芯片的第一基板的第一表面的平面结构示意图，图5为图1A和图1B所示的检测芯片的第二基板的平面结构示意图。
例如，如图1A-图5所示，当检测芯片具有吸水膜170时，吸水膜170可以设置在反应膜150的远离第二基板102的一侧，即设置在反应膜150与检测凹槽141之间。
例如，第二基板102在对应于检测凹槽141的位置具有检测通孔160，进而可以通过检测通孔160对检测凹槽141内的反应膜150进行光学检测，以获取样品的检测指标。
例如，检测通孔160贯穿第二基板102，且检测通孔160的直径可以设置为2mm-10mm，进一步可以为0.5mm-4mm，例如可以设置为3mm。由此，既可以通过检测通孔160实现光学检测，又可以减弱或避免射向不同检测通孔160的光线之间彼此发生干扰，从而进一步提升光学检测的准确性和精确性。
例如，第二基板102上除检测通孔160以外的部分均可以设置为不透光部分，由此可以减弱或避免射向不同检测通孔160的光线之间彼此发生干扰，减弱或避免检测通孔160之间的光串扰。例如，可以通过染色等方式来形成第二基板102的不透明部分。
例如，也可以在第二基板102远离第一基板101的一侧设置一层遮光层，该遮光层覆盖第二基板102远离第一基板101的表面的除具有检测通孔160的位置以外的其他部分，即该遮光层覆盖第二基板102远离第一基板101的表面且暴露检测通孔160，从而同样可以减弱或避免射向不同检测通孔160的光线之间彼此发生干扰，减弱或避免检测通孔160之间的光串扰。例如，遮光层采用不透明材料，该遮光层可以通过例如粘贴、印刷等方式设置在第二基板102的表面，本公开实施例对形成遮光层的具体方式不作限制。
例如，上述遮光层也可以设置在第二基板102靠近第一基板101的一侧，即该遮光层覆盖第二基板102靠近第一基板101的表面且暴露检测通孔160，从而同样可以减弱或避免射向不同检测通孔160的光线之间彼此发生干扰。 或者，在本公开的一些实施例中，第二基板102远离第一基板101的一侧和第二基板102靠近第一基板101的一侧可以均设置一层遮光层，该两层遮光层分别覆盖第二基板102远离和靠近第一基板101的表面的除具有检测通孔160的位置以外的其他部分，由此可以更好地减弱或避免射向不同检测通孔160的光线之间彼此发生干扰，进而减弱或避免检测通孔160之间的光串扰。
例如，在本公开的其他一些实施例中，检测芯片的第二基板上也可以不开设检测通孔，此时，第二基板可以采用允许光线通过的透明材料，从而可以直接通过第二基板对检测凹槽内的反应膜进行光学检测。
例如，第二基板可以采用允许光线通过的透明材料，并且在第二基板远离第一基板的表面上通过印刷或粘贴不透明的遮光层而形成检测窗口，以允许光线仅由检测窗口通过。例如，在一些实施例中，遮光层也可以设置在第二基板靠近第一基板的一侧的表面上，或者第二基板靠近第一基板的一侧的表面和远离第一基板的一侧的表面上可以同时设置有遮光层，本公开的实施例对此不作限制。本公开实施例对第二基板采用的具体材料不作限制，只要满足可以通过第二基板或第二基板上开设的检测通孔对第一基板的检测凹槽内的反应膜进行光学检测即可。
需要说明的是，在本公开实施例中，第一基板101上设置的检测凹槽141以及第二基板102上设置的与检测凹槽141对应的检测通孔160的数量只是示例性说明，本公开实施例对检测凹槽141以及检测通孔160的具体个数不作限制。例如，第一基板101上可以设置有1-20个检测凹槽141，相应地，第二基板102上可以设置有1-20个检测通孔160。
例如，第二基板102的材料可以包括聚甲基丙烯酸甲酯、聚苯乙烯、聚碳酸酯和聚对苯二甲酸乙二醇酯中的一种或多种。例如，第一基板101的材料可以包括聚甲基丙烯酸甲酯、聚苯乙烯和聚碳酸酯中的一种或多种，或者也可以为其他具有高透光性能的材料等。本公开的实施例对第一基板101和第二基板102的材料不做具体限定。
例如，第一基板101与第二基板102可以通过键合(指两片材料通过范德华力、分子力以及原子力等使两片材料键合成为一体的技术)、焊接、粘结或卡接的方式结合，进而简化检测芯片10的例如加工、组装等工艺，降低检测芯片10的制备成本。例如，第一基板101与第二基板102可以通过超声键合、热压键合、激光焊接、超声焊接或硅胶密封等工艺结合。本公开实施例对第一基板101和第二基板102之间的具体结合方式不作限制。
例如，第一基板101的形状可以为圆形、矩形或其他适合的形状，第二基板102的形状同样可以为圆形、矩形或其他适合的形状。
例如，在第一基板101的形状为圆形的情形，第一基板101的直径可以为3cm-15cm，进一步可以为3cm-5cm。由于在使用时，检测芯片10需要 与例如检测装置相配合，上述大小的第一基板101可以合理减小检测芯片10所需占据的空间，使检测芯片10实现薄型化设计，从而有助于检测芯片10与检测装置的配合使用，并且使检测芯片10的运输、包装、储存等成本降低。例如，在第二基板102的形状为圆形的情形，第二基板102的直径可以为3cm-15cm，进一步可以为3cm-5cm。由于在使用时，检测芯片10需要与例如检测装置相配合，上述大小的第二基板102可以合理减小检测芯片10所需占据的空间，使检测芯片10实现薄型化设计，从而有助于检测芯片10与检测装置的配合使用，并且使检测芯片10的运输、包装、储存等成本降低。
例如，在第一基板101的形状为矩形的情形，第一基板101的对角线可以为3cm-15cm，进一步可以为3cm-5cm。例如，矩形的第一基板101可以简化检测芯片10的制备工艺，降低检测芯片10的加工工艺及精度要求，从而降低检测芯片10的制备成本。在第二基板102的形状为矩形的情形，第二基板102的对角线可以为3cm-15cm，进一步可以为3cm-5cm。例如，矩形的第二基板102可以简化检测芯片10的制备工艺，降低检测芯片10的加工工艺及精度要求，从而降低检测芯片10的制备成本。
例如，第一基板101和第二基板102的形状及尺寸可以彼此相同或相似，以便于第一基板101和第二基板102的结合。
例如，第一基板101的厚度可以为0.5mm-10mm，例如3mm或者5mm等。例如，第二基板102的厚度可以为0.1mm-5mm，例如0.5mm或者2mm等。由此，可以合理减小检测芯片10所需占据的空间，使检测芯片10实现薄型化设计，从而使检测芯片10的运输、包装、储存等成本降低，并且有助于检测芯片10与检测装置的配合使用。此外，上述数值范围还有助于检测芯片10的加工及制备，并且便于用户单手抓握、利用设备进行固定或拾取等。
例如，在一个示例中，第一基板101和第二基板102的直径为3.5cm，第一基板101的厚度为1.5mm，第二基板102的厚度为1mm。由此，在使检测芯片10实现薄型化设计且便于用户例如单手抓握、利用设备进行固定或拾取的同时，还可以简化检测芯片10的制备工艺，降低检测芯片10的加工工艺及精度要求，从而有助于检测芯片10的加工及制备。
例如，如图1A-图5所示，在一些实施例中，检测芯片10还可以包括加样凸出部190。加样凸出部190从第一基板101的第一表面沿远离第二基板102的方向突出，加样凸出部190的一端与加样开口110相接，另一端可以与大气连通。加样凸出部190可以起到样品容纳以及导流等作用，以使样品通过加样凸出部190快速流向加样开口110，从而便于向加样开口110内注入样品，增加检测芯片10的样品容积，并且便于检测芯片10的例如拿取及放置等。
例如，加样凸出部190包括圆锥形腔体，且使加样开口110与大气连通。 例如，圆锥形腔体的圆锥角度(例如圆锥的中心线与侧壁之间的夹角)可以为30°-75°，容积为50μL-200μL。例如，加样凸出部190的圆锥形腔体包括彼此相对的第一端和第二端，第一端与加样开口110相接且直径为0.5mm-5mm，第二端的直径为1mm-20mm。加样凸出部190的圆锥形腔体突出第一基板101的高度为1mm-20mm。由此，在检测芯片10与例如检测装置配合使用时，可以使加样凸出部190占据合理的空间以与检测装置的尺寸相匹配，从而有助于检测芯片10与检测装置的配合使用。此外，由上述数值范围确定的加样凸出部190可以更好地起到样品容纳以及导流等作用，例如使样品可以均匀且以合理的速度流向加样开口110，从而有助于向加样开口110内注入样品。
例如，在一个示例中，加样凸出部190与加样开口110相接的一端的直径为3mm，另一端的直径为10mm，加样凸出部190突出第一基板101的高度为1.5mm。由此，既可以使样品通过加样凸出部190快速流向加样开口110，又有助于检测芯片10的例如拿取、放置以及与例如检测装置的配合使用等。同时，还可以简化加样凸出部190的制备工艺，从而降低加工工艺及精度要求。
例如，在一些实施例中，如图4所示，加样开口110包括第一主体111以及从第一主体111向导流槽130凸出的第一凸出部112，第一凸出部112与导流槽130连通，以促进样品通过第一凸出部112快速流入导流槽130内。
例如，第一主体111的直径可以设置为1mm-10mm，例如5mm或者7mm等。由此，既可以保证样品能够均匀快速地流入导流槽130内，又可以保证检测凹槽141所需的空间。
例如，第一主体111的形状可以为圆形，也可以为正方形或其他适合的形状，第一凸出部112可以呈正方形、尖角形或其他形状，本公开的实施例对此不作限制。例如，当第一主体111的形状为方形时，第一主体111的对角线长可以设置为1mm-10mm，例如5mm或者7mm等。例如，圆形或方形的第一主体111可以简化检测芯片10的制备工艺，降低检测芯片10的加工工艺及精度要求，从而降低检测芯片10的制备成本。
例如，导流槽130的内壁可以设置为具有亲水性，例如液体在导流槽130的内壁上的接触角小于90°，由此有助于样品快速进入导流槽130内，并使样品可以通过导流槽130迅速流入检测凹槽141内与反应膜150混合，进而缩短样品的检测时间，提升获得的检测数据的准确性。例如，在制备检测芯片的过程中，可以将亲水处理剂注入导流槽130中，使导流槽130被亲水处理剂浸泡1分钟后再用空气将亲水处理剂排出，使得导流槽130的内壁可以设置为具有亲水性。
需要说明的是，液体在导流槽130的内壁上的接触角是指沿液滴表面的 切线与导流槽130的内壁表面所形成的夹角。例如，当液体在导流槽130的内壁上的接触角小于90°时，则可以认为导流槽130的内壁具有亲水性。液体在导流槽130的内壁上的接触角的数值越小，则导流槽130的内壁的润湿性越好，例如当液体在导流槽130的内壁上的接触角为0°时，导流槽130的内壁材料被完全润湿。本公开的实施例对液体在导流槽130的内壁上的接触角的具体数值不作限制，只要满足该接触角小于90°以使导流槽130的内壁具有亲水性即可。
例如，在一些实施例中，导流槽130的高度可以设置为0.1mm-1.5mm，例如0.5mm；导流槽130的宽度可以设置为0.1mm-2mm，例如0.5mm。由此，有助于控制通过导流槽130流入检测凹槽141内的样品量，减少或避免样品在流动过程中的浪费。
例如，在一些实施例中，导流槽130的高度与导流槽130的宽度的比值可以设置为1:1-10:1，例如2:1。由此，在一些实施例中，当第一基板101和第二基板102通过粘结方法结合时，例如将第一基板101的第一表面和第二基板102通过粘结剂粘结，此时，该粘结剂可以采用疏水材料，由于第一基板101的第一表面与第二基板102的粘结面之间具有疏水的粘结剂，因此通过增大导流槽130的高度与导流槽130的宽度的比值，可以减小样品在流经导流槽130时与第二基板102的粘结面上的疏水粘结剂的接触面积，进而实现样品在导流槽130内的快速流动。
例如，在一个示例中，导流槽130的高度为1mm，导流槽130的宽度为0.5mm。由此，既有助于实现样品在导流槽130内的快速流动，又可以减少或避免样品在流动过程中的浪费，同时还可以简化导流槽130的制备工艺，降低加工工艺及精度要求。
需要说明的是，在一些实施例中，导流槽130的第二端(即与检测凹槽141连通的一端)与反应膜150接触，从而有助于样品浸入到反应膜150中，使样品与反应膜150中的检测试剂充分反应，例如，反应膜150的一部分可以位于导流槽130中。而在一些实施例中，反应膜150也可以不与导流槽130的第二端接触，本公开的实施例对此不作限制。
例如，在本公开实施例中，检测芯片10的例如加样凸出部190、加样开口110、导流槽130、检测凹槽141等结构均可以通过例如注塑工艺一体形成，从而简化检测芯片10的制备工艺。
例如，在一些实施例中，反应膜150包括基体材料以及分布在基体材料中的检测试剂，基体材料可以包括玻璃纤维、棉纤维或玻璃纤维和棉纤维的复合纤维等材料。
例如，在一些实施例的检测芯片中，第一基板101和第二基板102相结合的状态下，反应膜150沿厚度方向处于被两个基板压缩的压缩状态。例如， 反应膜150处于松弛状态时的厚度可以等于或略高于检测凹槽141的高度，例如反应膜150处于松弛状态时的厚度与检测凹槽141的高度的比值可以为1:1-1:0.5，进而在将第一基板101与第二基板102结合后，由于反应膜150沿厚度方向被压缩，反应膜150面向第二基板102的一侧的表面与第一基板101的第一表面可以位于同一水平面上，从而反应膜150与第二基板102紧密接触，可避免反应膜150与第一基板101或第二基板102之间产生缝隙而造成存液现象。由此，进入的样品可以更充分浸入到反应膜150中并与反应膜150中的检测试剂更充分反应，提升获得的检测数据的准确性。
例如，在本公开的一些实施例中，反应膜150的压缩量可以设置为10％-40％，从而有助于使反应膜150与第二基板102紧密接触，进而减弱或避免反应膜150与第一基板101或第二基板102之间产生缝隙而造成存液现象。
例如，反应膜150的形状通常与检测凹槽141的形状相同或相似，以便于反应膜150容纳在检测凹槽141内，并且有助于反应膜150与样品之间混合均匀。
例如，图6示出了图1A和图1B所示的检测芯片的反应膜的一种示例性结构。
例如，如图4和图6所示，在一些实施例中，反应膜150包括膜主体151和第二凸出部152。膜主体151与检测凹槽141的形状相同，且均为圆形。第二凸出部152从膜主体151向导流槽130凸出，第二凸出部152的至少部分位于导流槽130中，进而可以有助于将样品从导流槽130引入到检测凹槽141内，并使样品可以充分浸入到反应膜150中，使样品与反应膜150中的检测试剂充分反应，提升获得的检测数据的准确性。
例如，检测凹槽141的直径可以设置为3mm-15mm，反应膜150的膜主体151的直径等于或小于检测凹槽141的直径。由此，使反应膜150能够更好地容纳在检测凹槽141内，从而有助于反应膜150与样品之间混合均匀。
例如，在本公开的其他一些实施例中，反应膜的整体也可以设置为与检测凹槽的形状相同，即反应膜可以只具有膜主体部分而不具有凸出部分(例如第二凸出部152)，本公开实施例对此不作限制。
例如，在本公开的其他一些实施例中，反应膜还可以设置为多边形，且多边形的一个角与导流槽的第二端直接连通。相应地，检测凹槽为与反应膜的形状相同或相似的多边形。
例如，反应膜可以设置为三角形、正方形、菱形等规则形状，也可以设置为不规则形状等，本公开的实施例对此不作限制。
例如，图7A和图7B分别为本公开至少一实施例提供的另一种检测芯片的正面透视图和背面透视图。例如，图7A为检测芯片的俯视图，图7B为检 测芯片的仰视图。图8为图7A和图7B中所示的检测芯片的爆炸图，也即分解图。
例如，如图7A-图8所示，检测芯片20包括加样开口210和多个检测分支结构220，多个检测分支结构220沿加样开口210的周边均匀分布。多个检测分支结构220中的每个包括导流槽230和检测部240。导流槽230具有第一端和第二端，第一端与加样开口210连通。检测部240包括检测凹槽241和反应试剂(例如反应膜250)，检测凹槽241与导流槽230的第二端连通，反应膜250容纳在检测凹槽241中，检测部240被配置为允许对位于检测凹槽241中的反应膜250进行光学检测。
例如，如图7A-图8所示，检测芯片20的反应膜250设置为多边形，例如正方形或者菱形，相应地，检测凹槽241的形状也设置为正方形或者菱形。
例如，检测凹槽241的边长可以设置为3mm-15mm，例如5mm或者10mm等，反应膜250的边长等于或小于检测凹槽241的边长。由此，使反应膜250能够更好地容纳在检测凹槽241内，从而有助于反应膜250与样品之间混合均匀。
例如，在检测凹槽341的形状设置为正方形的情形，正方形的对角线可以设置为1mm-20mm，例如10mm或者15mm等。例如，在检测凹槽341的形状设置为菱形的情形，菱形的两条对角线可以分别设置为1mm-20mm；例如，一条对角线的长为10mm，而另一条对角线的长为6mm。例如，菱形的两条对角线之间的长度比例可以设置为1:1～1:2。例如，菱形的相邻两条边之间的夹角可以设置为小于或等于60°，从而有助于样品沿反应膜250的周边充分地浸入到反应膜250中，使样品可以与反应膜250中的检测试剂充分反应，进而提升获得的检测数据的准确性。
例如，反应膜250的一个角与导流槽230的第二端直接连通，以将样品从导流槽230引入到检测凹槽241内，并使样品可以充分浸入到反应膜250中，使样品与反应膜250中的检测试剂充分反应，提升获得的检测数据的准确性。
图9A和图9B分别为图7A和图7B所示的检测芯片的第一基板的正面透视图和背面透视图。例如，图9A为第一基板201的俯视图，图9B为第一基板201的仰视图。图10为图7A和图7B所示的检测芯片的第一基板的第一表面的平面结构示意图。
例如，结合图7A-图10所示，检测芯片20还包括第一基板201和第二基板202。第一基板201具有相对的第一表面和第二表面，加样开口210为第一基板201中的通孔，导流槽230和检测凹槽241形成在第一基板201的第一表面。第二基板202层叠在第一基板201的第一表面上且在对应于检测凹槽241的位置允许进行光学检测。
例如，第二基板202在对应于检测凹槽241的位置具有检测通孔260，进而可以通过检测通孔260对检测凹槽241内的反应膜250进行光学检测，以获取样品的不同检测指标。
例如，在本公开实施例中，检测通孔260的形状为圆形，而在本公开的其他一些实施例中，检测通孔260的形状也可以设置为例如与检测凹槽241的形状相同的正方形、菱形或其他形状，本公开的实施例对此不作限制。
例如，如图10所示，加样开口210包括第一主体211以及从第一主体211向导流槽230凸出的第一凸出部212，第一凸出部212与导流槽230连通，以使样品通过第一凸出部212流入导流槽230内。
需要说明的是，第一基板201和第二基板202的材料、具体结构参数等可以参考上述实施例中关于检测芯片10的第一基板101和第二基板102的相应描述，在此不再赘述。检测芯片20的具体结构参数、材料及功能等可以参考上述实施例中关于检测芯片10的相应描述，在此不再赘述。
例如，在本公开的一些实施例中，检测芯片的检测部还可以包括位于检测凹槽周边的导流凹槽，例如导流凹槽可以至少部分围绕检测凹槽。导流凹槽形成在第一基板的第一表面且与检测凹槽连通，至少部分导流凹槽的高度小于检测凹槽的高度。由此，样品可以通过导流凹槽迅速地沿反应膜的部分周边区域浸润，在导流凹槽与反应膜之间对样品产生毛细力以起到引流的作用，使样品从反应膜的周边向反应膜的中心区域浸润，从而加快样品与反应膜之间的混合速度，进一步缩短检测时间。并且，在样品从反应膜的周边浸润的情形，由于毛细作用，会使浸润至反应膜的中心区域的样品量加大，进而使反应膜的中心区域的检测结果(例如显色)更为明显，便于后续观察检测结果，从而提升获得的检测数据的准确性。
例如，在本公开的一些实施例中，导流凹槽围绕检测凹槽的四周，即围绕检测凹槽的所有周边区域，使样品可以通过导流凹槽更加迅速地沿反应膜的周边浸润，再从反应膜的周边向反应膜的中心浸润，从而进一步加快样品与反应膜之间的混合速度，更加显著地缩短所需的检测时间。并且，在样品从反应膜的四周同时浸润的情形，由于毛细作用，浸润至反应膜的中心的样品量增加，进而使反应膜的中心的检测结果(例如显色)最为明显，便于后续检测结果的观察，从而进一步提升获得的检测数据的准确性。
需要说明的是，在本公的一些实施例中，导流凹槽还可以在垂直于第一基板的第一表面的方向上与检测凹槽彼此交叉或部分重叠，本公开的实施例对此不作限制。
例如，图11为本公开至少一实施例提供的再一种检测芯片的爆炸图，也即分解图。图12为图11所示的检测芯片的第一基板的结构示意图。例如，图12为图11所示的检测芯片30的第一基板301的仰视图。需要说明的是， 除储液通孔380和导流凹槽342外，图11所示的检测芯片30与图7A-图8所示的检测芯片20的结构及功能基本相同或相似，在此不再赘述。
例如，结合图11和图12所示，检测芯片30的检测部340包括反应试剂(例如反应膜350)、检测凹槽341和围绕检测凹槽341的导流凹槽342和343。
图13A为图12所示的检测芯片的第一基板的部分结构示意图，例如检测芯片30的第一基板301的包括检测部340的部分结构的结构示意图，图13B为图13A中沿A-A’线的截面结构示意图，图13C为图13A中沿B-B’线的截面结构示意图。需要说明的是，为了简洁、清楚，图12-图13C中未示出检测部340的反应膜350。
例如，如图11-图13C所示，导流凹槽342和343形成在第一基板301的第一表面3011且与检测凹槽341连通，例如，导流凹槽342的一端与导流槽330相接，另一端与导流凹槽343相接。由此，在样品通过导流槽330流入检测部340时，样品会先流入导流凹槽342和343内并通过导流凹槽342和343迅速地沿反应膜350的四周浸润，再从反应膜350的四周向反应膜350的中心浸润，从而加快样品与反应膜350之间的混合速度，缩短所需的检测时间。并且，由于毛细作用，使浸润至反应膜350的中心的样品量加大，进而使反应膜350的中心的检测结果(例如显色)更为明显，便于后续检测结果的观察，从而提升获得的检测数据的准确性。
例如，结合图13A和图13B所示，导流凹槽342与检测凹槽341的纵截面整体呈阶梯状，由此使导流凹槽342与反应膜350之间可以形成较大的供样品流动的间隙，从而进一步提升样品在导流凹槽342内的流动速度，以实现样品可以迅速地沿反应膜350的四周浸润，再从反应膜350的四周向反应膜350的中心浸润，进而改善反应膜350的中心的浸润效果，使反应膜350的中心的检测结果(例如显色)更为明显。
例如，结合图13A和图13B所示，导流凹槽342的高度H2小于检测凹槽341的高度H1。例如，检测凹槽341的高度H1可以设置为0.2mm-5mm，例如2mm或者3mm等，由此在第一基板301和第二基板302相结合的状态下，反应膜350沿厚度方向处于被两个基板压缩的压缩状态，使反应膜350面向第二基板302的一侧的表面与第一基板301的第一表面3011可以位于同一水平面上，从而使反应膜350与第二基板302紧密接触，可避免反应膜350与第一基板301或第二基板302之间产生缝隙而造成存液现象。导流凹槽342的高度H2与检测凹槽341的高度H1的差值为0.1mm-1mm，例如0.5mm或者0.8mm等，由此使样品可以在检测凹槽341内快速流动，从而使样品更加迅速地沿反应膜350的周边浸润。
例如，导流凹槽342的宽度D1可以设置为0.1mm-1mm，例如0.5mm 或者0.7mm等。由此，使导流凹槽342与反应膜350之间形成足够且适当的供样品流动的间隙，进而有助于样品从反应膜350的四周向反应膜350的中心浸润，改善反应膜350的中心的浸润效果，使反应膜350的中心的检测结果(例如显色)更为明显。
例如，在检测凹槽341的形状设置为正方形的情形，正方形的对角线可以设置为1mm-20mm，例如10mm或者15mm等。例如，在检测凹槽341的形状设置为菱形的情形，菱形的两条对角线可以分别设置为1mm-20mm；例如，一条对角线的长为10mm，而另一条对角线的长为6mm。例如，菱形的两条对角线之间的长度比例可以设置为1:1～1:2。例如，菱形的相邻两条边之间的夹角可以设置为小于或等于60°，从而有助于样品沿反应膜250的四周向反应膜350的中心浸润，使样品可以充分地浸入到反应膜250中，进而提升获得的检测数据的准确性。
例如，在本公开的其他一些实施例中，导流凹槽342还可以设计为包括多层台阶结构的阶梯状，本公开的实施例对此不作限制。
例如，结合图13A和图13C所示，导流凹槽343设置为斜坡状结构。例如，导流凹槽343包括呈斜坡状的导流壁344，导流壁344相对于第一基板301的第一表面3011倾斜，导流壁344的第一端3441与第一基板301的第一表面3011相接，导流壁344的第二端3442与检测凹槽341的侧表面相接。由于相比于导流凹槽342，导流凹槽343与反应膜350之间形成的供样品流动的间隙较小，因而样品在导流凹槽343内的流动速度稍微小于样品在导流凹槽342内的流动速度。由此，样品通过导流凹槽342流入导流凹槽343后，可以减弱或避免样品过多地存留在导流凹槽343内，从而进一步提升样品从反应膜350的四周向反应膜350的中心浸润的速度，使浸润至反应膜350的中心的样品量加大，进而使反应膜350的中心的检测结果(例如显色)更为明显，更加有利于后续检测结果的观察。
需要说明的是，在本公开的其他一些实施例中，导流凹槽343也可以设置为其他适合的结构，只要满足使导流凹槽343与反应膜350之间形成的供样品流动的空间小于或等于导流凹槽342与反应膜350之间形成的供样品流动的空间即可，本公开的实施例对此不作限制。
例如，结合图12、图13A和图13C所示，导流壁344的第二端3442不平行于第一基板301的第一表面3011，例如导流壁344的第二端3442相对于第一基板301的第一表面3011倾斜且沿样品的流动方向(例如沿远离加样开口310的方向)朝第一基板301的第一表面3011延伸。也就是说，导流壁344的表面积(也即，导流壁344与样品之间的接触面积)沿样品的流动方向逐渐减小，例如沿远离加样开口310的方向逐渐减小，从而使导流凹槽343与反应膜350之间形成的供样品流动的空间沿样品的流动方向逐渐减小。由 此，进一步提升样品从反应膜350的四周向反应膜350的中心浸润的速度，使浸润至反应膜350的中心的样品量在一定程度上进一步加大，进而使反应膜350的中心的检测结果(例如显色)更为明显，更加有利于后续检测结果的观察，从而提升获得的检测数据的准确性。
需要说明的是，在本公开的其他一些实施例中，导流壁344也可以设置为其他适合的轮廓形状，只要满足使导流壁344与样品之间的接触面积沿样品的流动方向逐渐减小即可，本公开的实施例对此不作限制。
例如，导流凹槽343的最大高度H4小于检测凹槽341的高度H1，即导流壁344与检测凹槽341的侧表面相接的第二端与第一基板301的第一表面3011之间的距离小于检测凹槽341的高度H1。由此，可以使导流凹槽343与反应膜350之间形成供样品流动的间隙，使样品可以迅速地沿反应膜350的四周浸润，再从反应膜350的四周向反应膜350的中心浸润，从而改善反应膜350的中心的浸润效果，使反应膜350的中心的检测结果(例如显色)更为明显。
例如，导流壁344的第二端与第一基板301的第一表面3011之间的距离与检测凹槽341的高度的差值(即H4与H1的差值)可以设置为0.1mm-1mm。由此，可以在导流凹槽343与反应膜350之间形成适当的供样品流动的间隙，从而改善反应膜350的中心的浸润效果。
例如，导流凹槽343的宽度D2可以设置为0.1mm-1mm，例如0.5mm或者0.7mm等，由此使导流凹槽343与反应膜350之间形成适当的供样品流动的间隙，从而改善反应膜350的中心的浸润效果。
需要说明的是，在图11-图13C所示的上述实施例中，导流凹槽342的宽度D1与导流凹槽343的宽度D2相同，导流凹槽342的高度H2与导流凹槽343的最大高度H4相同；而在本公开的其他一些实施例中，导流凹槽342的宽度D1与导流凹槽343的宽度D2也可以彼此不同，导流凹槽342的高度H2与导流凹槽343的最大高度H4也可以彼此不同，本公开的实施例对此不作限制。
需要说明的是，在图11-图13C所示的上述实施例中，围绕检测凹槽341的导流凹槽包括结构彼此不同的导流凹槽342和导流凹槽343，而在本公开的其他一些实施例中，围绕检测凹槽341的导流凹槽也可以均采用相同的结构，例如均设置为导流凹槽342，或者均设置为导流凹槽343，本公开的实施例对此不作限制。
需要说明的是，在图11-图13C所示的上述实施例中，以在菱形检测凹槽的周边开设导流凹槽为例，对导流凹槽的结构进行了说明。在本公开的其他一些实施例中，在检测凹槽为三角形、圆形等其他规则形状或不规则形状的情形，同样也可以在相应的检测凹槽的周边开设导流凹槽，例如，可以在 图1A-图2B所示的检测芯片10的检测凹槽141的周边开设导流凹槽，本公开的实施例对此不作限制。
例如，在本公开实施例中，导流凹槽342和343围绕检测凹槽341且导流凹槽342和343的轮廓形状与检测凹槽341的轮廓形状相同，而在本公开的其他一些实施例中，导流凹槽342和343的轮廓形状也可以与检测凹槽341的轮廓形状不同，本公开的实施例对此不作限制。
例如，在本公开的其他一些实施例中，导流凹槽也可以部分围绕检测凹槽，例如以菱形的检测凹槽为例，可以仅对应检测凹槽与导流槽相邻的两边设置导流凹槽，以实现样品与反应膜之间的快速混合。同时，还可以使反应膜上靠近中心的某一区域或某一范围内的检测结果(例如显色)更为明显，进而便于后续观察检测结果，提升获得的检测数据的准确性。
例如，如图12和图13A所示，导流槽330伸入检测凹槽341内，导流槽330与检测凹槽341连通的第二端位于菱形的检测凹槽341内，从而使样品可以更加快速且充分地浸润反应膜350，缩短所需的检测时间。
例如，导流槽330的第二端伸入检测凹槽341内的长度小于或等于1.5mm，例如0.5mm或者1mm等。由此，可以有助于将样品从导流槽330引入到检测凹槽341内，使样品可以充分浸入到反应膜350中，从而使样品与反应膜350中的检测试剂充分反应，提升获得的检测数据的准确性。
例如，在本公开的一些实施例中，反应膜350沿厚度方向(即沿图13A或图13B中所示的高度H1的方向)处于压缩状态。例如，反应膜350处于松弛状态时的厚度可以等于或略高于检测凹槽341的高度H1，例如反应膜350处于松弛状态时的厚度与检测凹槽341的高度H1的比值可以为1:1-1:0.5，进而在将第一基板301与第二基板302结合后，由于反应膜350沿厚度方向被压缩，反应膜350与第二基板302紧密接触，从而避免反应膜350与第一基板301或第二基板302之间产生缝隙而造成存液现象。
例如，如图11和图12所示，检测部340还可以包括贯穿第一基板301且与检测凹槽341连通的储液通孔380，进而在通过加样开口310注入的样品量过多的情形，多余的样品可以储存在储液通孔380中，从而避免或减弱由于样品注入过多而导致的漏液现象，并且储液通孔380还可以促进样品通过导流凹槽342和343从反应膜350的周边向中心浸润，以使反应膜350的中心的检测结果(例如显色)更为明显。
例如，储液通孔380的直径可以设置为0.2mm-5mm，例如2mm或者3mm等，从而有利于控制样品量，避免或减弱由于样品注入过多而导致的漏液现象，促进样品从反应膜350的周边向中心浸润，并且还可以降低储液通孔380的加工工艺及精度要求，进而简化制备工艺。
例如，如图13B和图13C所示，储液通孔380的深度H3与检测凹槽341 的高度H1的比值可以设置为0.5:1-10:1，例如3:1等，从而起到样品定量的作用，有利于控制样品量，同时储液通孔380还可以存储更多样品，防止样品溢出。
例如，储液通孔380连通检测凹槽341的中心，即储液通孔380开设在检测凹槽341的底面的中心位置，将检测凹槽341的中心与外部环境例如大气连通，进而更好地促进样品从反应膜350的周边向中心浸润。
例如，在检测芯片30的制造过程中，为了便于检测芯片30的组装，在组装时，可以在储液通孔380处加入负压，以使反应膜350固定在检测凹槽341中。由此，样品可以与反应膜350中的检测试剂更充分反应，进一步提升获得的检测数据的准确性。
例如，在本公开的一个示例中，检测凹槽341的形状为菱形且菱形的两条对角线分别为8mm和5.8mm,检测凹槽341的高度H1为0.5mm,导流凹槽342的高度H2与检测凹槽341的高度H1的差值为0.3mm，导流凹槽343的最大高度H4与检测凹槽341的高度H1的差值为0.3mm，导流凹槽342的宽度D1为0.3mm，导流凹槽343的宽度D2为0.3mm，储液通孔380的直径为2.5mm,储液通孔380的深度H3为1mm。进而，有利于控制检测凹槽341内的样品量，避免或减弱由于样品注入过多而导致的漏液现象，同时促进样品从反应膜350的周边向中心浸润，使浸润至反应膜350的中心的样品量加大，进而使反应膜350的中心的检测结果(例如显色)更为明显，更加有利于后续检测结果的观察。
需要说明的是，在本公开的其他一些实施例中，储液通孔还可以开设在检测凹槽的底面的其他位置；或者，根据实际需要，每个检测部内也可以开设多个储液通孔。本公开实施例对储液通孔的设置位置以及数量等均不作限制。
图14为本公开至少一实施例提供的再一种检测芯片的爆炸图，也即分解图。图14为检测芯片的俯视图，示出的是从检测芯片正面观看到的结构。需要说明的是，除粘结层704和加样凸出部790外，图14所示的检测芯片70与图11所示的检测芯片30的结构及功能基本相同或相似，在此不再赘述。
例如，图14示出了检测芯片70通过粘结层704将第一基板701和第二基板702结合的情形。
例如，如图14所示，粘结层704层叠在第一基板701和第二基板702之间，即层叠在第一基板701的第一表面上。
例如，粘结层704可以采用例如具有疏水性的粘性材料，例如双面胶等。
例如，如图14所示，粘结层704包括与第一基板701的检测凹槽以及导流凹槽(未示出)相对应的开口7041，由此可以避免由于粘结层704表面不平整以及自身粘性等而对样品流动产生的阻碍，同时也可以避免粘结层704 与反应膜750之间发生粘结，从而有助于样品在检测凹槽和导流凹槽内的流动，使样品能够快速且充分地浸润反应膜750，提升获取的检测结果的准确性。
例如，在本公开的一些实施例中，开口7041的形状及尺寸可以与检测凹槽的横截面的形状及尺寸相同，从而减弱或避免粘结层704对样品流动产生的阻碍，或者减弱或避免粘结层704与反应膜750之间发生粘结。在本公开的一些实施例中，也可以是开口7041的形状与检测凹槽的横截面的形状相同，开口7041的大小稍微小于检测凹槽的横截面的大小，从而稍微加大粘结层704与第一基板701和第二基板702之间的接触面积，以提升第一基板701和第二基板702之间的粘结性，由此进一步减弱或避免样品漏出，提升检测芯片70的密封效果。
例如，开口7041的尖端部分(例如顶角部分)可以设置为直角，或者也可以设置为带有一定的弧度的圆角，例如可以为直角型或圆弧型。例如，以图14中的菱形开口7041为例，菱形的四个角可以设置为直角也可以设置为带有一定的弧度的圆角，本公开的实施例对此不作限制。
例如，在一些示例中，以图14中的菱形开口7041为例，可以将菱形的两组对角分为第一组和第二组，并将第一组对角设置为直角，第二组对角设置为呈圆弧型。例如，由于菱形开口7041的形状与第一基板701的检测凹槽的轮廓形状基本相同，通过使第一组对角呈圆弧型，可以使菱形开口7041的大小略小于检测凹槽的横截面的大小，进而在一定程度上加大粘结层704与第一基板701和第二基板702之间的接触面积，改善第一基板701和第二基板702之间的粘结效果；而通过将第二组对角设置为直角，可以在一定程度上减弱或避免粘结层704对样品流动产生的阻碍，同时还可以减弱或避免粘结层704与反应膜750之间发生粘结。
由此，通过设置上述第一组对角和第二组对角，既可以减弱或避免粘结层704因自身粘性等而对样品流动产生阻碍以及与反应膜750之间发生粘结等问题，还可以更好地实现第一基板701与第二基板702之间的结合，减弱或避免样品漏出，提升检测芯片70的密封效果。
需要说明的是，在其他的一些示例中，也可以是开口7041的所有顶角全部设计为直角，或者全部设计为呈圆弧型或其他适合的轮廓，本公开的实施例对此不作限制。
需要说明的是，在图14所示的实施例中，开口7041的形状及尺寸与第一基板701的检测凹槽及导流凹槽的轮廓形状及尺寸基本相同，而在本公开的其他一些实施例中，粘结层的开口的尺寸也可以稍大于或稍小于检测凹槽或导流凹槽的尺寸，或者也可以设置为其他合适的形状或尺寸，只要可以避免或减弱粘结层对样品浸润反应膜产生阻碍且保证检测芯片的密封效果即 可，本公开实施例对此不作限制。
例如，如图14所示，相比于图11所示的检测芯片30，检测芯片70采用横截面呈花瓣状的加样凸出部790，由此可以增大加样凸出部790与第一基板701之间的接触面积，有助于加样凸出部790与第一基板701之间的稳固连接，并且更适宜用户单手拿握、利用设备进行固定或拾取等。
例如，如图14所示，第一基板701包括第一凹口7011，第二基板702包括与第一凹口7011对应的第二凹口7021，在垂直于第一基板701或第二基板702的表面的方向上，第一凹7011和第二凹口7021彼此重叠。第一凹口7011和第二凹口7021形成限位部(或称为限位块)，从而使检测芯片70通过由第一凹口7011和第二凹口7021形成的限位块可以固定于例如检测装置上，进而便于对检测芯片70中的反应膜750进行检测，提升获取的检测数据的准确性。此外，通过第一凹口7011和第二凹口7021的位置还可以对检测芯片70中的多个检测分支结构进行标号和定位，进而便于检测数据的例如观察和记录等。
需要说明的是，第一凹7011和第二凹口7021的尺寸可以根据需要与其进行固定的例如检测装置的结构而确定，本公开的实施例对此不作限制，只要满足检测芯片70可以通过第一凹7011和第二凹口7021固定于检测装置上即可。
例如，在垂直于第一基板701或第二基板702的表面的方向上，第一凹7011和第二凹口7021可以设置在相邻的检测凹槽之间，或者其他适合的位置，本公开的实施例对此不作限制。
例如，在检测芯片70包括粘结层704或其他结构层或功能层的情形，该结构层或功能层上在对应于第一凹口7011和第二凹口7021的位置也具有凹口结构，以使检测芯片70整体形成限位块。
例如，在本公开的一些实施例中，检测芯片的加样开口、导流槽、检测凹槽等结构也可以采用其他方式设置，只要能实现加样开口、导流槽、检测凹槽等结构的布置并实现相应的功能即可。上述实施例是以加样开口贯穿第一基板且导流槽、检测凹槽等结构设置在检测芯片的第一基板的第一表面的情形进行的介绍，但这并不构成对本公开的限制。
下面在图11所示的检测芯片30的基础上，对导流槽和检测凹槽的其他可行的设置位置进行示例性说明。
例如，图15A和图15B为本公开至少一实施例提供的再一种检测芯片的爆炸图，也即分解图。例如，图15A为检测芯片的俯视图，图15B为检测芯片的仰视图。需要说明的是，图15A和图15B所示的检测芯片40中具体部件的结构及功能等可以参考上述实施例中关于检测芯片10、检测芯片20或检测芯片30的相应描述，在此不再赘述。
例如，如图15A和图15B所示，检测芯片40包括第一基板401、第二基板402和第三基板403。
例如，第一基板401具有相对的第一表面(图中示出为上表面)和第二表面(图中示出为下表面)，导流槽430形成在第一基板401的第二表面，检测凹槽441形成在第一基板401的第一表面。第二基板402层叠在第一基板401的第一表面上且在对应于检测凹槽441的位置具有检测通孔460，以允许进行光学检测。第三基板403层叠在第一基板401的第二表面上，以密封导流槽430和加样开口410，使样品可以由加样开口410流入导流槽430。
例如，加样开口410为贯穿第一基板401的通孔，第二基板402具有暴露加样开口410的通孔，进而使样品可以注入加样开口410内，并且使加样凸出部490可以从第二基板402伸出，以便于检测芯片40的例如拿取及放置。
例如，图16为本公开至少一实施例提供的再一种检测芯片的爆炸图，也即分解图。例如，图16为检测芯片的俯视图。需要说明的是，图16所示的检测芯片50中具体部件的结构及功能等可以参考上述实施例中关于检测芯片10、检测芯片20或检测芯片30的相应描述，在此不再赘述。
例如，如图16所示，检测芯片50包括第一基板501和第二基板502。第一基板501具有相对的第一表面(图中示出为上表面)和第二表面(图中示出为下表面)，导流槽530和检测凹槽541形成在第一基板501的第一表面，第二基板502层叠在第一基板501的第一表面上且在对应于检测凹槽541的位置具有检测通孔560以允许进行光学检测。
例如，加样开口510为第一基板501中的开孔，该开孔例如为未贯穿第一基板501的非通孔，此时，加样开口510的高度(或深度)小于第一基板501的厚度。第二基板502具有暴露加样开口510的通孔和加样凸出部590，以使样品可以通过加样凸出部590注入加样开口510内，且便于检测芯片50的例如拿取及放置。
本公开至少一个实施例还提供一种检测系统，该检测系统包括：检测装置和本公开任一实施例提供的检测芯片，例如上述实施例中的检测芯片10、检测芯片20、检测芯片30、检测芯片40或检测芯片50，检测装置配置为通过检测芯片的检测部对检测凹槽中的反应膜进行检测。
例如，以上述实施例中的检测芯片10为例，图17为本公开至少一实施例提供的一种检测系统的示意图。
例如，如图17所示，检测系统60包括检测芯片10和检测装置610，检测装置610配置为通过检测芯片10的检测部140对检测凹槽141中的反应试剂，例如反应膜150，进行检测。
例如，检测装置610包括光源611和光电检测装置612。光源611配置为向反应膜150发光，光电检测装置612配置为接收从光源611发出且被反 应膜150反射的光。
例如，光电检测装置612可以将被反应膜150反射的光的强度与光源611发出的光的强度进行比较，从而根据检测的反应膜150的吸光度的数值来判断样品中待检物的有无以及浓度等，实现对样品指标的检测。例如，以反应膜150上预先滴定的检测试剂为显色试剂为例，在获取检测结果时，反应膜150上显现的颜色越深，则说明被检测样品中待检物的含量越高，相应地利用光电检测装置612检测到的反应膜150的吸光度的数值也越大。
例如，在一些实施例中，光电检测装置612可以为光电二极管，光电二极管可以将接收的光信号转换为电信号，进而可以根据电信号中电参数的变化(例如电流的变化等)来判断接收的光的强度，从而确定反应膜150的吸光度的数值。
本公开实施例提供的检测系统的具体说明及技术效果可以参考本公开实施例提供的检测芯片中的相应内容，例如可以参考上述实施例中的检测芯片10、检测芯片20、检测芯片30、检测芯片40或检测芯片50的相应内容，在此不再赘述。
还有以下几点需要说明：
(1)本公开实施例的附图只涉及到与本公开实施例涉及到的结构，其他结构可参考通常设计。
(2)为了清晰起见，在用于描述本公开的实施例的附图中，层或区域的厚度被放大或缩小，即这些附图并非按照实际的比例绘制。可以理解，当诸如层、膜、区域或第一基板之类的元件被称作位于另一元件“上”或“下”时，该元件可以“直接”位于另一元件“上”或“下”或者可以存在中间元件。
(3)在不冲突的情况下，本公开的实施例及实施例中的特征可以相互组合以得到新的实施例。
以上，仅为本公开的具体实施方式，但本公开的保护范围并不局限于此，本公开的保护范围应以权利要求的保护范围为准。

Claims (44)

  1. 一种检测芯片，包括：
    加样开口，
    至少一个检测分支结构，其中，所述至少一个检测分支结构中的每个包括：
    检测部，包括检测凹槽和反应试剂，
    其中，所述检测凹槽与所述加样开口连通，所述反应试剂容纳在所述检测凹槽中，
    所述检测部被配置为允许对位于所述检测凹槽中的所述反应试剂进行光学检测。
  2. 根据权利要求1所述的检测芯片，其中，所述至少一个检测分支结构中的每个还包括导流槽，
    所述导流槽具有第一端和第二端，所述导流槽的第一端与所述加样开口连通，所述导流槽的第二端与所述检测凹槽连通，以使所述检测凹槽通过所述导流槽与所述加样开口连通。
  3. 根据权利要求2所述的检测芯片，还包括：
    第一基板，具有第一表面，其中，所述加样开口为所述第一基板中的通孔，所述导流槽和所述检测凹槽形成在所述第一基板的第一表面；
    第二基板，层叠在所述第一基板的第一表面上且在对应于所述检测凹槽的位置允许进行所述光学检测。
  4. 根据权利要求3所述的检测芯片，其中，所述至少一个检测分支结构中的每个还包括吸水膜，
    所述吸水膜容纳在所述检测凹槽中，且与所述反应试剂在垂直于所述第一基板的方向上彼此至少部分层叠。
  5. 根据权利要求4所述的检测芯片，其中，所述吸水膜设置在所述反应试剂的远离所述第二基板的一侧。
  6. 根据权利要求4或5所述的检测芯片，其中，所述吸水膜的液体存储量为10μL-50μL。
  7. 根据权利要求2-6任一所述的检测芯片，其中，所述检测部还包括位于所述检测凹槽周边的导流凹槽，所述导流凹槽与所述检测凹槽连通；
    至少部分所述导流凹槽的高度小于所述检测凹槽的高度。
  8. 根据权利要求7所述的检测芯片，其中，所述导流凹槽包括呈斜坡状的导流壁，
    所述导流壁的一端与所述检测凹槽的侧表面相接。
  9. 根据权利要求7所述的检测芯片，其中，所述导流凹槽与所述检测凹 槽的纵截面整体呈阶梯状。
  10. 根据权利要求7-9任一所述的检测芯片，其中，所述导流凹槽至少部分围绕所述检测凹槽。
  11. 根据权利要求7-10任一所述的检测芯片，其中，所述检测凹槽的高度为0.2mm-5mm，所述导流凹槽的最大高度与所述检测凹槽的高度的差值为0.1mm-1mm，所述导流凹槽的宽度为0.1mm-1mm。
  12. 根据权利要求3-6任一所述的检测芯片，其中，所述检测部还包括贯穿所述第一基板且与所述检测凹槽连通的储液通孔。
  13. 根据权利要求12所述的检测芯片，其中，所述储液通孔连通所述检测凹槽的中心。
  14. 根据权利要求12或13所述的检测芯片，其中，所述储液通孔的直径为0.2mm-5mm，所述储液通孔的深度与所述检测凹槽的高度的比值为0.5:1-10:1。
  15. 根据权利要求2-14任一所述的检测芯片，其中，所述导流槽的高度为0.1mm-1.5mm，所述导流槽的宽度为0.1mm-2mm。
  16. 根据权利要求2-15任一所述的检测芯片，其中，所述导流槽的高度与所述导流槽的宽度的比值为1:1-10:1。
  17. 根据权利要求2-16任一所述的检测芯片，其中，所述导流槽的内壁具有亲水性。
  18. 根据权利要求2-17任一所述的检测芯片，其中，所述至少一个检测分支结构包括多个检测分支结构，所述多个检测分支结构沿所述加样开口的周边均匀分布。
  19. 根据权利要求2-18任一所述的检测芯片，其中，所述加样开口包括第一主体以及从所述第一主体向所述导流槽凸出的第一凸出部，所述第一凸出部与所述导流槽连通。
  20. 根据权利要求19所述的检测芯片，其中，所述第一主体的直径为1mm-10mm。
  21. 根据权利要求2-20任一所述的检测芯片，其中，所述反应试剂包括反应膜和/或块状反应试剂。
  22. 根据权利要求21所述的检测芯片，其中，所述反应膜沿厚度方向处于压缩状态。
  23. 根据权利要求22所述的检测芯片，其中，所述反应膜处于松弛状态时的厚度与所述检测凹槽的高度的比值为1:1-1:0.5。
  24. 根据权利要求21-23任一所述的检测芯片，其中，所述反应膜为圆形，或者，
    所述反应膜为多边形，且所述多边形的一个角与所述导流槽的第二端直 接连通。
  25. 根据权利要求21-23任一所述的检测芯片，其中，所述反应膜包括膜主体以及从所述膜主体向所述导流槽凸出的第二凸出部，所述第二凸出部的至少部分位于所述导流槽中；
    所述膜主体与所述检测凹槽的形状相同，为圆形。
  26. 根据权利要求21-23任一所述的检测芯片，其中，所述反应膜为菱形。
  27. 根据权利要求24所述的检测芯片，其中，所述检测凹槽的直径为3mm-15mm，所述反应膜的直径等于或小于所述检测凹槽的直径。
  28. 根据权利要求3-6和12-14任一所述的检测芯片，其中，所述第二基板在对应于所述检测凹槽的位置具有检测通孔。
  29. 根据权利要求28所述的检测芯片，其中，所述第二基板不透光；或者，
    所述检测芯片还包括遮光层，所述遮光层覆盖所述第二基板远离和/或靠近所述第一基板的表面，且暴露所述检测通孔。
  30. 根据权利要求28或29所述的检测芯片，其中，所述检测通孔的直径为2mm-10mm。
  31. 根据权利要求3-6、12-14和28-30任一所述的检测芯片，其中，所述第一基板的材料包括聚甲基丙烯酸甲酯、聚苯乙烯和聚碳酸酯中的一种或多种。
  32. 根据权利要求3-6、12-14和28-31任一所述的检测芯片，其中，所述第二基板的材料包括聚甲基丙烯酸甲酯、聚苯乙烯、聚碳酸酯和聚对苯二甲酸乙二醇酯中的一种或多种。
  33. 根据权利要求3-6、12-14和28-32任一所述的检测芯片，其中，所述第一基板与所述第二基板通过键合、焊接、粘结或卡接的方式结合。
  34. 根据权利要求33所述的检测芯片，还包括粘结层；
    其中，所述粘结层位于所述第一基板和所述第二基板之间且被用于使所述第一基板和所述第二基板结合，
    所述粘结层包括对应于所述检测凹槽的开口。
  35. 根据权利要求2-34任一所述的检测芯片，其中，所述反应试剂包括基体材料以及分布在所述基体材料中的检测试剂，
    所述基体材料包括玻璃纤维、棉纤维或玻璃纤维和棉纤维的复合纤维。
  36. 根据权利要求2所述的检测芯片，还包括：
    第一基板，具有第一表面，其中，所述加样开口为所述第一基板中的通孔，所述导流槽和所述检测凹槽形成在所述第一基板的第一表面；
    第二基板，层叠在所述第一基板的第一表面上，并且在对应于所述检测 凹槽的位置具有检测通孔，以允许通过所述检测通孔进行所述光学检测；
    其中，所述至少一个检测分支结构包括多个检测分支结构，所述多个检测分支结构沿所述加样开口的周边均匀分布。
  37. 根据权利要求2所述的检测芯片，还包括：
    第一基板，具有相对的第一表面和第二表面，其中，所述加样开口为所述第一基板中的通孔，所述检测凹槽形成在所述第一基板的第一表面，所述导流槽形成在所述第一基板的第二表面；
    第二基板，层叠在所述第一基板的第一表面上且在对应于所述检测凹槽的位置允许进行所述光学检测；
    第三基板，层叠在所述第一基板的第二表面上且密封所述加样开口和所述导流槽。
  38. 根据权利要求2所述的检测芯片，还包括：
    第一基板，具有第一表面，其中，所述加样开口、所述导流槽和所述检测凹槽形成在所述第一基板的第一表面，所述加样开口为所述第一基板中的非通孔；
    第二基板，层叠在所述第一基板的第一表面上且暴露所述加样开口，并在对应于所述检测凹槽的位置允许进行所述光学检测。
  39. 根据权利要求2-38任一所述的检测芯片，还包括光学校准分支结构，
    其中，所述光学校准分支结构包括光路检测区，所述光路检测区被配置为进行光学校准。
  40. 根据权利要求3-6、12-14和28-34任一所述的检测芯片，还包括加样凸出部，
    其中，所述加样凸出部从所述第一基板的第一表面沿远离所述第二基板的方向突出，
    所述加样凸出部的一端与所述加样开口相接。
  41. 根据权利要求40所述的检测芯片，其中，所述加样凸出部包括圆锥形腔体，
    所述圆锥形腔体的容积为50μL-200μL，所述圆锥形腔体突出所述第一基板的高度为1mm-20mm；
    所述圆锥形腔体包括彼此相对的第一端和第二端，所述第一端与所述加样开口相接，所述第一端的直径为0.5mm-5mm，所述第二端的直径为1mm-20mm。
  42. 根据权利要求3-6、12-14、28-34、40和41任一所述的检测芯片，其中，所述第一基板包括第一凹口，所述第二基板包括与所述第一凹口对应的第二凹口，
    所述第一凹口和所述第二凹口被用于固定所述检测芯片。
  43. 一种检测系统，包括：
    权利要求1-42任一所述的检测芯片，
    检测装置，配置为通过所述检测部对所述检测凹槽中的所述反应试剂进行检测。
  44. 根据权利要求43所述的检测系统，其中，所述检测装置包括：
    光源，配置为向所述反应试剂发光，
    光电检测装置，配置为接收从所述光源发出且被所述反应试剂反射的光。
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