WO2022062919A1 - 冰箱 - Google Patents

冰箱 Download PDF

Info

Publication number
WO2022062919A1
WO2022062919A1 PCT/CN2021/117714 CN2021117714W WO2022062919A1 WO 2022062919 A1 WO2022062919 A1 WO 2022062919A1 CN 2021117714 W CN2021117714 W CN 2021117714W WO 2022062919 A1 WO2022062919 A1 WO 2022062919A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample
microfluidic
detection
biochip
detection system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2021/117714
Other languages
English (en)
French (fr)
Inventor
朱小兵
费斌
孙永升
刘浩泉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qingdao Haier Refrigerator Co Ltd
Haier Smart Home Co Ltd
Original Assignee
Qingdao Haier Refrigerator Co Ltd
Haier Smart Home Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Haier Refrigerator Co Ltd, Haier Smart Home Co Ltd filed Critical Qingdao Haier Refrigerator Co Ltd
Priority to US18/246,486 priority Critical patent/US11883819B2/en
Priority to EP21871291.7A priority patent/EP4206569B1/en
Publication of WO2022062919A1 publication Critical patent/WO2022062919A1/zh
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D23/00—General constructional features
    • F25D23/12—Arrangements of compartments additional to cooling compartments; Combinations of refrigerators with other equipment, e.g. stove
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/52—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
    • B01L9/527—Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips for microfluidic devices, e.g. used for lab-on-a-chip
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D23/00—General constructional features
    • F25D23/02—Doors; Covers
    • F25D23/028—Details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0609—Holders integrated in container to position an object
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • the invention relates to refrigeration and freezing technology, in particular to a refrigerator.
  • the existing detection systems generally exist independently, take up space, and are inconvenient to store. After the detection device is stored, it will be forgotten to be used, or it will not be taken out for use because it is troublesome.
  • the existing pesticide residue detection system integrated on the refrigerator detects the gas volatilized from the food or the condensed water flowing down from the food. It not only needs to arrange a gas collection or liquid collection device, which leads to a very complicated structure and a large space occupation, which affects the normal operation of users. Storage space, and the detection accuracy is very low, which greatly affects the user experience.
  • One object of the present invention is to overcome at least one defect of the prior art, and to provide a refrigerator integrated with a microfluidic detection system with a simple structure.
  • a further object of the present invention is to improve the operational convenience for users to replace the microfluidic biochip.
  • Another further object of the present invention is to attenuate the vibration of the microfluidic detection system, reduce its operating noise, and improve its heat dissipation efficiency.
  • the present invention provides a refrigerator, comprising a microfluidic detection system for qualitatively or quantitatively detecting preset detection parameters of a sample liquid, the microfluidic detection system comprising:
  • the microfluidic biochip has a sample inlet, a communication port, and a detection pool formed inside the sample inlet, the detection pool, and the communication port are sequentially communicated through a microfluidic channel;
  • a sample liquid driving device in sealing communication with the communication port, so as to facilitate the sample liquid in contact with the sample inlet to flow into the microfluidic channel and to flow to the detection cell through the microfluidic channel;
  • a detection mechanism is used to detect the detection cell to obtain preset detection parameters of the sample liquid.
  • a fluid-tight connection is formed between the sample liquid driving device and the communication port through a sealing docking mechanism
  • the communication port of the microfluidic biochip is fixedly provided with a plug-in pin that protrudes and extends outward, the internal flow channel of the plug-in needle is in sealing communication with the communication port, and the plug-in needle is inserted into
  • the interior of the sealing and docking mechanism forms a fluid-tight connection with the sealing and docking mechanism, and the sealing and docking mechanism is in fluid-tight connection with the sample liquid driving device, so that the sample liquid driving device is sealed with the communication port Connected.
  • the end surface of the extension end of the plug-in needle is a continuous and smooth hemispherical surface, and the needle hole of the plug-in needle for fluid communication with the sealing butt-joint mechanism is formed at the position of the plug-in needle. Circumferential sides of the segment inside the sealed abutment mechanism.
  • the microfluidic detection system further includes:
  • the chip ejection mechanism is used to operably release the support effect of the chip mounting mechanism on the microfluidic biochip, so as to release the microfluidic biochip.
  • the chip mounting mechanism includes two oppositely arranged elastic clamping claws, so as to apply an opposing force to the microfluidic biochip located between the two elastic clamping claws, so that the microfluidic The biological control chip is clamped between the two elastic clamping jaws; and
  • the chip ejection mechanism is configured to operably apply a force in opposite directions to the two elastic jaws, so as to cause the two elastic jaws to produce elastic deformation in a direction away from each other, thereby releasing the two elastic jaws The gripping action of the gripper on the microfluidic biochip.
  • the microfluidic detection system further includes:
  • the sample stage is configured for controlled or operable movement to transport a sample cup placed thereon through the sample stage to allow the sample fluid in the sample cup to interact with the microfluidic biochip the position where the injection port is in contact.
  • the microfluidic biochip is disposed above the sample stage, and the injection port is located at the bottom of the microfluidic biochip;
  • the microfluidic detection system further includes a lifting mechanism for driving the sample stage to move up and down, so that the sample liquid in the sample cup allowed to be placed on the sample stage and the sample inlet are connected to the sample stage. Switching between the detection position of the contact and the initial position which is a preset distance below the detection position.
  • the microfluidic detection system further includes:
  • Buffer bottles for holding buffers
  • a buffer driving device communicated with the buffer bottle, to controllably drive the buffer in the buffer bottle into the sample cup placed on the sample stage, so that the buffer is connected to the sample cup
  • the samples in the mixture are mixed to produce the sample solution.
  • the sample liquid driving device is disposed adjacently to the lateral side of the microfluidic biochip, and includes a driving motor disposed in the air.
  • the microfluidic detection system further includes:
  • the sample table is used for placing the sample cup, and the sample cup is used for holding the sample liquid;
  • the sample stand includes a support stand for supporting the sample cup and a shaker provided on the support stand, and the shaker is used to oscillate the sample cup after the sample cup is placed on the support stand , so that the sample solution is generated after the buffer solution and the sample in the sample cup are fully mixed.
  • the microfluidic detection system further includes:
  • the chip mounting mechanism, the sample liquid driving device, the detection mechanism and at least a part of the microfluidic biochip are all disposed in the casing;
  • the casing is provided with a structural connector for connecting with the box or door of the refrigerator, and an electrical connection for forming an electrical connection between the microfluidic detection system and the electrical control device of the refrigerator.
  • the refrigerator further includes:
  • a box body defining a storage space for storing items therein;
  • a door body connected with the box body, and used for opening and/or closing the storage space;
  • the microfluidic detection device is arranged on the door body.
  • the refrigerator of the present invention includes a microfluidic detection system, and the microfluidic detection system includes a microfluidic biochip for providing detection conditions and a detection environment, a chip mounting mechanism for installing the microfluidic biochip, and a microfluidic biochip for accurately A sample liquid driving device for controlling the flow of the sample liquid into the microfluidic biochip, and a detection mechanism for performing a detection operation.
  • the combination of the structure and function of these four modules can not only perform sample injection and detection operations smoothly, but also ensure the accuracy of detection results through precise control of sample injection. On this basis, the structure of each module is very simple, which simplifies the structure of the microfluidic detection system and avoids occupying too much space on the refrigerator.
  • the microfluidic detection system also includes a chip ejection mechanism, and the user can release the support function of the chip mounting mechanism and the microfluidic biochip by operating the chip ejection mechanism, thereby releasing the microfluidic biochip, and the user can easily take out the microfluidic biochip.
  • the fluidic biochip or the microfluidic biochip can be dropped under the action of its own gravity, which simplifies the user's operation process and improves the convenience of the user to replace the microfluidic biochip.
  • the sample liquid driving device has a driving motor, and the driving motor is suspended in the air and does not contact with other structures, which avoids the vibration generated by the driving motor from being transmitted to the microfluidic biochip or other structures, thereby weakening the entire microfluidic detection.
  • the vibration of the system reduces its operating noise.
  • the driving motor since the driving motor is used at a relatively high frequency and generates a relatively large amount of heat, the suspended arrangement of the driving motor also increases the size of the surrounding space, which is beneficial to heat dissipation.
  • FIG. 1 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention.
  • FIG. 2 is a schematic structural diagram of a microfluidic detection system according to an embodiment of the present invention.
  • FIG. 3 is a schematic structural exploded view of a microfluidic detection system according to an embodiment of the present invention.
  • FIG. 4 is a schematic structural diagram of the internal structure of a microfluidic detection system according to an embodiment of the present invention.
  • FIG. 5 is a schematic structural exploded view of the internal structure of a microfluidic detection system according to an embodiment of the present invention.
  • FIG. 6 is a schematic cross-sectional view of a microfluidic biochip according to an embodiment of the present invention.
  • FIG. 7 is a schematic cross-sectional view of a sample liquid driving device and a microfluidic biochip and a structure for connecting the two according to an embodiment of the present invention
  • FIG. 8 is a schematic cross-sectional exploded view of a sealed docking mechanism and a microfluidic biochip according to another embodiment of the present invention.
  • Fig. 9 is a schematic enlarged view of part A in Fig. 8.
  • FIG. 10 is a schematic structural diagram of a microfluidic biochip, a chip mounting mechanism and a chip ejecting mechanism according to an embodiment of the present invention
  • FIG. 11 is a schematic structural diagram of the lifting mechanism and the sample stage in an exploded state according to an embodiment of the present invention.
  • FIG. 12 is a schematic structural exploded view of a door body according to an embodiment of the present invention.
  • FIG. 1 is a schematic structural diagram of a refrigerator according to an embodiment of the present invention.
  • the refrigerator 100 according to the present invention includes a box body 200 and a door body 300 .
  • the box body 200 defines a storage space for storing articles
  • the door body 300 is connected with the box body 200 and is used to open and/or close the storage space thereof.
  • the refrigerator 100 further includes a microfluidic detection system 1, and the microfluidic detection system 1 is used to perform qualitative or quantitative detection on preset detection parameters of the sample liquid, for example, the preset detection parameters may be used to indicate the amount of pesticide residues Whether it exceeds the standard and/or the specific value of the pesticide residue parameter, the nutritional parameter used to indicate whether the nutrient element meets the standard and/or the specific content of the nutrient element, whether the specific harmful substance (such as a specific virus) exceeds the standard and/or Specific content of specific substance parameters and so on.
  • the preset detection parameters may be used to indicate the amount of pesticide residues Whether it exceeds the standard and/or the specific value of the pesticide residue parameter, the nutritional parameter used to indicate whether the nutrient element meets the standard and/or the specific content of the nutrient element, whether the specific harmful substance (such as a specific virus) exceeds the standard and/or Specific content of specific substance parameters and so on.
  • FIG. 2 is a schematic structural diagram of a microfluidic detection system according to an embodiment of the present invention
  • FIG. 3 is a schematic structural exploded view of a microfluidic detection system according to an embodiment of the present invention
  • FIG. 4 is a schematic structural diagram of a microfluidic detection system according to an embodiment of the present invention
  • FIG. 5 is a schematic structural exploded view of the internal structure of the microfluidic detection system according to an embodiment of the present invention.
  • the sample cup 2 is also shown in FIGS. 1 to 5 .
  • the microfluidic detection system 1 may include a microfluidic biochip 10 , a chip mounting mechanism 51 , a sample liquid driving device 40 and a detection mechanism 20 .
  • a detection mechanism 20 may be different.
  • the microfluidic biochip 10 when used for pesticide residue detection, can be a microfluidic pesticide residue detection chip that can provide detection conditions for the pesticide residue liquid, and the detection mechanism 20 it has can be It is a pesticide residue testing institution that can detect the pesticide residue parameters of pesticide residue liquid.
  • the microfluidic biochip 10 has an injection port 111, a communication port 112, and a detection cell 121 formed inside the microfluidic biochip 10.
  • the injection port 111 , the detection cell 121 , and the communication port 112 are sequentially communicated through the microfluidic channel 14 , thereby allowing the sample liquid in contact with the injection port 111 to flow into the microfluidic channel 14 and into the detection pool 121 through the microfluidic channel 14 .
  • the microfluidic channel 14 referred to in the present invention refers to a microfluidic channel or a capillary channel with a flow area within a predetermined size range, so that it has a suitable ability to retain the liquid therein.
  • the injection port 111 and the communication port 112 may be formed at the end of the microfluidic biochip 10 . Further, the injection port 111 and the communication port 112 are preferably formed at different ends of the microfluidic biochip 10 .
  • the chip mounting mechanism 51 is used for mounting the microfluidic biochip 10 to provide support for the microfluidic biochip 10 .
  • the sample liquid driving device 40 is in sealing communication with the communication port 112, so as to promote the sample liquid in contact with the sample inlet 111 to flow into the microfluidic channel 14 and flow to the detection cell 121 through the microfluidic channel 14, so as to prevent the sample liquid entering the detection cell 121.
  • volume and flow rate are precisely controlled.
  • the detection mechanism 20 is used to detect the detection cell 121 to obtain preset detection parameters of the sample liquid. Specifically, detection reagents may be pre-installed in the detection cell 121, or the detection reagents may be added to the detection cell 121 manually or automatically, so that the sample liquid in the detection cell 121 reacts with the detection reagents in the detection cell 121 to pass the detection The mechanism 20 detects the detection cell 121 .
  • the refrigerator 100 of the present invention includes a microfluidic detection system 1, and the microfluidic detection system 1 includes a microfluidic biochip 10 for providing detection conditions and a detection environment, and a chip mounting mechanism for installing the microfluidic biochip 10. 51.
  • the sample liquid driving device 40 for precisely controlling the flow of the sample liquid into the microfluidic biochip 10, and the detection mechanism 20 for performing the detection operation.
  • the combination of the structure and function of these four modules can not only perform sample injection and detection operations smoothly, but also ensure the accuracy of detection results through precise control of sample injection. On this basis, the structure of each module is very simple, which simplifies the structure of the microfluidic detection system 1 and prevents it from occupying too much space on the refrigerator 100.
  • the microfluidic biochip 10 further includes a reaction cell 122 formed in the interior thereof.
  • the reaction cell 122 is located on the main channel formed by the injection port 111, the detection cell 121, and the communication port 112 connected in sequence, and is connected to the injection port 112. between the port 111 and the detection cell 121 , so that the sample liquid first reacts with the reaction reagent in the reaction cell 122 and then flows into the detection cell 121 .
  • the microchannel 14 communicates between the reaction cell 122 and the sample inlet 111 and between the reaction cell 122 and the detection cell 121 .
  • the reaction reagents and detection reagents for pesticide residue detection can be enzyme reagents and chromogenic reagents, respectively.
  • the reaction cell 122 is used for reacting the sample liquid with the enzyme reagent therein, and the sample liquid reacted with the enzyme reagent flows into the detection cell 121 to react with the color developing agent in the detection cell 121 .
  • the detection mechanism 20 may be selected as a photoelectric detection mechanism, which may include a light source 21 and a photosensitive element 22 respectively disposed on two opposite sides of the microfluidic biochip 10 and facing the detection cell 121, and the light emitted by the light source 21 It is irradiated to the detection cell 121, and the light transmitted through the detection cell 121 is guided into the photosensitive element 22, so as to facilitate the determination of the absorbance change in the detection cell 121 through the light intensity signal received by the photosensitive element 22, and then calculate the pesticide residue inhibition rate. Further, the detection mechanism 20 also includes a heating sheet 24 for providing heat to the detection cell 121 and a thermostat 25 for controlling the heating power of the heating sheet 24 to be constant, so that the sample liquid and detection reagent in the detection cell 121 are sufficient, Respond quickly.
  • a photoelectric detection mechanism which may include a light source 21 and a photosensitive element 22 respectively disposed on two opposite sides of the microfluidic biochip 10 and facing the detection cell 121, and the light emitted by the
  • the sample liquid driving device 40 may be adjacently disposed on the lateral side of the microfluidic biochip 10 , thereby ensuring the structure between the microfluidic biochip 10 and the sample liquid driving device 40
  • the compactness of the layout allows the liquid to flow down or drip down the microfluidic biochip 10 when the liquid in the microfluidic biochip 10 leaks or discharges without contacting the sample liquid driving device 40 .
  • adverse effects on the sample liquid driving device 40 are avoided.
  • the sample liquid driving device 40 may include a driving motor 41 which is suspended in the air. That is to say, the drive motor 41 is only connected to the support structure through its top, and does not contact other structures, which prevents the vibration generated by the drive motor 41 from being transmitted to the microfluidic biochip 10 or other structures, which not only prevents the microfluidic
  • the stability or performance of the control biochip 10 or other structures is adversely affected, and the vibration of the entire microfluidic detection system 1 is also reduced, and its operating noise is reduced.
  • the driving motor 41 is used frequently and generates a large amount of heat, the suspended arrangement of the driving motor 41 also increases the size of the surrounding space, which is beneficial to heat dissipation.
  • the sample liquid driving device 40 may form a negative pressure in the main channel by drawing air outward, so that the sample liquid in contact with the sample inlet 111 enters the main channel under the action of negative pressure.
  • FIG. 7 is a schematic cross-sectional view of a sample liquid driving device, a microfluidic biochip and a structure for connecting the two according to an embodiment of the present invention.
  • the sample liquid driving device 40 can be a micro syringe pump, and further includes a vertically extending syringe 42 , a screw rod 43 , a slider 44 and a piston 45 .
  • the syringe 42 is fixed on a bracket 87 , and the top of the syringe 42 is in sealing communication with the communication port 112 on the top of the microfluidic biochip 10 through the connecting pipeline 46 .
  • the screw rod 43 extends vertically and is connected with the driving motor 41 to rotate under the driving of the driving motor 41 .
  • the slider 44 is inserted through the screw rod 43 and is threadedly connected with the screw rod 43 to translate up and down along the screw rod 43 with the rotation of the screw rod 43 .
  • the piston 45 is arranged inside the syringe 42, and is fixedly connected with the slider 44 to translate in the up-down direction under the driving of the slider 44, so that when it translates downward, a negative pressure is generated in the main channel to promote the connection with the injection port.
  • the sample liquid contacted by 111 flows into the microfluidic channel and flows into the detection cell 121 through the microfluidic channel, and when it translates upward, the sample liquid in the main channel is caused to flow toward the injection port 111 .
  • the sample liquid driving device 40 may further include a position sensor for controlling the displacement amount of the upward translation and/or downward translation of the piston 45 in cooperation with the driving motor 41, thereby realizing precise control of sample injection.
  • the displacement amount of the upward translation and downward translation of the piston 45 can also be monitored in real time, so that it can perform a subtle pushing and pumping operation, so as to ensure that the sample liquid does not flow out through the communication port 112.
  • the sample liquid is pushed and sucked in opposite directions, so that the sample liquid in the detection cell 121 is more uniformly mixed or reacted more fully with the detection reagent in the detection cell 121, and the accuracy of the detection result is improved.
  • a fluid-tight connection is formed between the sample fluid drive device 40 and the communication port 112 through the sealing docking mechanism 90 .
  • the sealing butting mechanism 90 may include a sealing connecting member 91 and an elastic pressing member 92 .
  • the sealing connector 91 is connected between the microfluidic biochip 10 and the sample liquid driving device 40 , and a connection channel penetrating the sealing connector 91 is formed therein.
  • the elastic pressing member 92 is used to apply an elastic force to the sealing connection member 91, so that the sealing connection member 91 is sealed and docked with the sample liquid driving device 40 and the microfluidic biochip 10 at the same time, so that the sample liquid driving device 40 and the microfluidic biochip 10 are sealed and docked.
  • the communication port 112 of the control biochip 10 is sealedly communicated through the connection channel inside the sealing connector 91 . Therefore, an elastic force can be applied to the sealing connector 91 through the elastic pressing member 92, so that the sealing connector 91 is always kept in a state of tightly sealing and docking with the sample liquid driving device 40 and the microfluidic biochip 10 at the same time, so as to avoid
  • the use of other docking mechanisms may lead to problems such as loosening and breaking after long-term use, thereby ensuring a long-term and reliable fluid-tight communication relationship between the sample liquid driving device 40 and the communication port 112 of the microfluidic biochip 10.
  • the sealing effect between the two is improved.
  • the communication between the sample liquid driving device 40 and the communication port 112 is through the connecting line 46 .
  • the communication port 112 can be formed on the top of the microfluidic biochip 10 , and the sample liquid driving device 40 can be adjacent to the side of the microfluidic biochip 10 in the lateral direction to avoid possible leakage of the microfluidic biochip 10 .
  • the liquid adversely affects the sample liquid driving device 40 .
  • the connecting pipeline 46 can be communicated with the top of the sample liquid driving device 40 to bridge between the sample liquid driving device 40 and the microfluidic biochip 10 .
  • the sealing connector 91 may include a first connection block 912 for directly docking with the microfluidic biochip 10 and a second connection block 913 disposed on the side of the first connection block 912 away from the microfluidic biochip 10 .
  • a sealed connection is formed between the first connection block 912 and the second connection block 913 and between the second connection block 913 and the connection pipeline 46 in a plug-in manner.
  • the elastic pressing member 92 may be a spring, one end of the spring abuts against a fixed end plate 513 , and the other end abuts against the sealing connector 91 .
  • the end plate 513 and the microfluidic biochip 10 are respectively On opposite sides of the sealing connector 91 . Specifically, when the microfluidic biochip 10 is in a mounted state, the spring is in a compressed state, thereby generating an elastic force for urging the sealing connector 91 to move toward the microfluidic biochip 10 .
  • the number of the elastic pressing members 92 can be two or more, so as to increase the size of the elastic force acting on the microfluidic biochip 10, and make the elastic force on the microfluidic biochip 10 more balanced, so as to avoid the occurrence of The inclination further improves the effect of the sealing connection.
  • the sealing and docking mechanism 90 further includes a guide rod 93, and a spring is sleeved on the guide rod 93 to avoid displacement of the spring.
  • One end of the guide rod 93 is fixedly connected with the sealing connector 91, and the other end is in contact with a Hall switch 94 after the microfluidic biochip 10 is sealed with the sealing connector 91, thereby prompting the Hall switch 94 to generate a signal for indicating microfluidics.
  • FIG. 8 is a schematic cross-sectional exploded view of a sealed docking mechanism and a microfluidic biochip according to another embodiment of the present invention
  • FIG. 9 is a schematic enlarged view of part A in FIG. 8 .
  • FIG. 8 structures such as the elastic pressing member and the guide rod of the sealing butt joint mechanism are hidden.
  • the communication port 112 of the microfluidic biochip 10 of the present invention is also fixedly connected with a plug pin 15 that protrudes outward, and the internal flow channel 151 of the plug pin 15 is connected to the communication port. 112 is in sealing communication, and the pin 15 is inserted into the interior of the sealing interface 90 and forms a fluid-tight connection with the sealing interface 90 . That is, the sealing and docking mechanism 90 is fluidly connected to the sample liquid driving device 40 and the plug pin 15 at the same time, so as to realize a good sealing communication relationship between the sample liquid driving device 40 and the communication port 112 of the microfluidic biochip 10 .
  • the plug pin 15 can be plugged into the sealing connector 91 of the sealing butt mechanism 90 .
  • the sealing connector 91 in the embodiment shown in FIGS. 8 and 9 has a slightly different structure from the sealing connector 91 in the embodiment shown in FIG. 7 .
  • the bottom of the first connection block 912 of the sealing connector 91 in the embodiment shown in FIG. 8 and FIG. 9 may be provided with through holes 9121 for inserting the plug pins 15 , and the through holes 9121 and the plug pins 15 can pass through the contact hole 9121 . Sealing fit by means of contact or squeeze contact.
  • a needle hole 152 for fluidly connecting its internal flow channel 151 with the interior of the sealing butt mechanism 90 may be opened on the plug needle 15, and the needle hole 152 is opened on the section of the plug needle 15 that is inside the sealing butt mechanism 90, That is to say, the needle hole 152 of the plug-in needle 15 is located inside the sealing and docking mechanism 90, which not only ensures a smooth and good fluid communication relationship between the two, but also improves the sealing performance between the two to a large extent, avoiding There are problems such as air leakage and liquid leakage at the connection between the two.
  • the structural strength of the sealing butt For materials with high structural strength, after the plug-in pin 15 is inserted and withdrawn for a limited number of times, the sealing butt joint mechanism 90 will still suffer structural damage. Therefore, the applicant of the present application improves the structure of the plug pin 15 from another perspective.
  • the end surface 153 of the extended end of the plug pin 15 is a continuous and smooth hemispherical surface, and the pin hole 152 of the plug pin 15 for fluid communication with the sealing butt mechanism 90 is formed in the plug pin 15 in the sealing butt joint. Circumferential side 154 of the segment inside mechanism 90 .
  • the contact surface between the plug pins 15 and the sealing docking mechanism 90 is a smooth spherical surface, reducing the size of the plug pins 15 and the sealing and docking mechanism 90, so the sealing and docking mechanism 90 will not be scratched or punctured, which ensures that the sealing and docking mechanism 90 maintains a good sealing and docking function for a long time, and improves the service life of the microfluidic detection system 1. , and at the same time, the requirement for the structural strength of the sealing butt joint mechanism 90 is also reduced.
  • the extension end of the plug pin 15 refers to the end of the plug pin 15 that protrudes into the sealing butt mechanism 90 .
  • the pin hole 152 can be formed on the circumferential side of the section of the splicing pin 15 adjacent to its extending end, whereby even if the section where the splicing pin 15 is inserted into the sealing abutment mechanism 90 is not long, the splicing pin 15 can still be ensured 15 in fluid communication with the sealing interface 90 .
  • the plug pin 15 is plugged into the microfluidic biochip 10 through the communication port 112, and the start end of the plug pin 15 extending into the microfluidic biochip 10 is open, so as to facilitate the operation of the microfluidic biochip 10. It communicates with the microfluidic channel 14 and thus communicates with the communication port 112 .
  • the mating interface between the plug pin 15 and the communication port 112 can be sealed by the sealant 16 to enhance the sealing performance between the plug pin 15 and the microfluidic biochip 10 .
  • the plug pins 15 can also be integrally formed with the microfluidic biochip 10 .
  • the microfluidic detection system 1 further includes a chip ejection mechanism 52 for operably releasing the support effect of the chip mounting mechanism 51 on the microfluidic biochip 10 to release the microfluidic biochip 10 .
  • a chip ejection mechanism 52 for operably releasing the support effect of the chip mounting mechanism 51 on the microfluidic biochip 10 to release the microfluidic biochip 10 .
  • the user can release the support function of the chip mounting mechanism 51 and the microfluidic biochip 10 by operating the chip ejection mechanism 52, thereby releasing the microfluidic biochip 10, and the user can easily take out the microfluidic biochip 10 or the microfluidic biochip 10.
  • the fluidic biochip 10 can be dropped under the action of its own gravity, which simplifies the user's operation process and improves the operational convenience for the user to replace the microfluidic biochip 10 .
  • the chip ejection mechanism 52 can be exposed outside the refrigerator 100 to facilitate the user to perform the chip ejection operation, regardless of the structural layout of the microfluidic detection system 1 itself and the overall structural layout of the integrated refrigerator The disassembly operation of the microfluidic biochip 10 is affected, and the user experience is improved.
  • the chip mounting mechanism 51 may include two oppositely arranged elastic clamping jaws 511 to apply an opposing force to the microfluidic biochip 10 between the two elastic clamping jaws 511 , so that the microfluidic The biochip 10 is clamped between the two elastic clamping jaws 511 .
  • the chip ejecting mechanism 52 is configured to operably apply a force in opposite directions to the two elastic clamping claws 511 to cause the two elastic clamping claws 511 to elastically deform in the direction away from each other, thereby releasing the two elastic clamping claws 511 The clamping effect on the microfluidic biochip 10 .
  • the chip ejection mechanism 52 may include a cantilever button 521 suspended on one side of the microfluidic biochip 10 , and a cantilever button 521 protruding toward the inner side of the microfluidic biochip 10 and gradually approaching the microfluidic biochip 10 .
  • the abutting block 522 extends out, and the abutting block 522 abuts against the oppositely disposed inner sides of the two elastic clamping jaws 511 at the same time, so that when the cantilever button 521 receives a force toward the microfluidic biochip 10, the abutting block 522 passes through the abutting block 522.
  • the two opposite sides of the abutting block which are respectively abutted against the inner sides of the two elastic clamping jaws 511, are inclined toward each other in the direction of gradually approaching the microfluidic biochip 10, so as to ensure the smoothness of the pressing operation of the cantilever button 521 and avoid the occurrence of jamming. etc. phenomenon.
  • the abutting block can be roughly in the shape of an isosceles trapezoid, the lower bottom of the isosceles trapezoid is connected to the cantilever button 521 , and the two waists of the isosceles trapezoid are in contact with the inner sides of the two elastic clamping claws 511 respectively.
  • the microfluidic detection system 1 further includes a sample stage 70, and the sample stage 70 is used to place the sample cup 2, and the sample cup 2 is used to hold the sample liquid.
  • the sample stage 70 is configured to be controlled or operable to move to transport the sample cup 2 placed thereon through the sample stage 70 to allow the sample liquid in the sample cup 2 to be injected with the microfluidic biochip 10 The position where the port 111 is in contact.
  • the sample loading of the microfluidic biochip 10 is realized.
  • the sample adding operation is very convenient, saving time and effort.
  • complex structures such as the sample liquid delivery pump, delivery pipeline, sampling needle, etc. are omitted, so that the structure of the microfluidic detection system 1 is very simple, thereby avoiding occupying the refrigerator. Too much space.
  • the microfluidic biochip 10 can be arranged above the sample stage 70 , and the sample inlet 111 is located at the bottom of the microfluidic biochip 10 so that the sample inlet 111 and the sample cup 2 placed on the sample stage 70 are connected to each other. sample fluid contact.
  • the microfluidic detection system 1 further includes a lifting mechanism 60 for driving the sample stage 70 to move up and down, so as to allow the sample liquid in the sample cup 2 placed on the sample stage 70 to come into contact with the sample inlet 111 for detection. Toggles between the position and the initial position which is a preset distance below the detection position. That is to say, the sample stage 70 can be automatically raised and lowered through the upgrading mechanism 60, which further simplifies the user's operation and improves the automation degree of the microfluidic detection system.
  • FIG. 11 is a schematic structural diagram of the lifting mechanism and the sample stage in an exploded state according to an embodiment of the present invention.
  • the lift mechanism 60 may include a lift motor 61 , a drive screw 62 and a nut 63 .
  • the lift motor 61 is used to output driving force.
  • the driving screw 62 is arranged in the vertical direction and is connected with the output shaft of the lifting motor 61 to rotate under the driving of the lifting motor 61 .
  • the nut 63 is threaded on the drive screw 62 and is threadedly connected with the drive screw 62 to move up and down along the drive screw 62 with the rotation of the drive screw 62 .
  • the sample stage 70 is fixedly connected with the nut 63, so as to drive the sample stage 70 to move up and down through the nut 63.
  • the lifting mechanism 60 further includes a sliding rail 64 and a sliding block 65 .
  • the sliding rail 64 is arranged on the side of the driving screw 62 in parallel with the driving screw 62
  • the sliding block 65 is movably arranged on the sliding rail 64
  • the sample stage 70 is fixedly connected with the sliding block 65 to pass the sliding rail 64 and the sliding block 65.
  • the cooperation of the slider 65 guides the sample stage 70 to move up and down. Specifically, when the sample stage 70 moves in the up-down direction under the action of the drive module, it drives the slider 65 to move synchronously. Therefore, the sample stage 70 is indirectly guided and limited, thereby avoiding the deviation or jamming of the sample stage 70 during the moving process, and improving the stability of the movement of the sample stage 70 .
  • the sample stage 70 may include a horizontal connecting plate 74 that penetrates the drive screw 62 and is fixedly connected to the nut 63 and a vertical connecting plate 75 that extends upward perpendicular to the horizontal connecting plate 74.
  • the vertical connecting plate 75 is connected to the sliding Block 65 is fixedly connected.
  • the elevating mechanism 60 further includes a limit switch 66 , and the limit switch 66 is disposed adjacent to the upper part of the driving screw 62 to cause the elevating motor 61 to stop when the sample stage 70 moves upward until it touches the limit switch 66 . run. Also, the position of the limit switch 66 is set so that the sample stage 70 is in its detection position when the lift motor 61 stops running under the triggering of the limit switch 66 . The sample stage 70 can be held in its detection position when the lift motor 61 is not operating.
  • the limit switch 66 is used to locate the detection position of the sample stage 70, and the positioning is accurate, which can avoid the problem that the sample stage 70, the microfluidic biochip 10 and other structures are damaged due to the continuous movement of the sample stage 70 beyond its detection position.
  • the sample stage 70 may include a support stage 71 and an oscillator 72 .
  • the support table 71 is used to support the sample cup 2 .
  • the support table 71 may be a horizontally placed support plate, and a groove for placing the bottom of the sample cup 2 therein may be provided on the support plate, so as to prevent the sample cup 2 from tipping or falling during the movement of the sample table 70 . Shaking improves the stability of placing the sample cup 2 .
  • the support table 71 is fixedly connected to the horizontal connecting plate 74 .
  • the oscillator 72 is arranged on the support table 71, and is used to oscillate the sample cup 2 after placing the sample cup 2 on the support table 71, so that the buffer solution and the sample in the sample cup 2 are fully mixed to generate a sample liquid, so that the sample The substance to be detected on it is fully dissolved in the buffer to obtain a sample solution with a suitable concentration.
  • the buffer solution can be preset in the sample cup 2 by manual addition, or can be automatically transported to the sample cup 2 by the driving device after the sample cup 2 is placed on the sample stage 70 .
  • the sample stage 70 further includes a load cell 73, and the load cell 73 is disposed under the support stage 71 to measure the weight of the sample in the sample cup 2, thereby allowing the buffer driving device 30 to interact with the A preset amount of buffer that matches the sample weight is delivered to sample cup 2.
  • the amount of buffer input in the sample cup 2 needs to be proportional to the amount of the sample. match, so that the sample solution of the appropriate concentration can be generated.
  • the weight of the sample can be obtained automatically and accurately through the load cell 73 placed under the support table 71, so as to automatically control the buffer driving device 30 to input a matching amount of buffer into the sample cup 2, which not only ensures the measurement result It also avoids many problems such as inconvenience, cumbersome operation, and large errors caused by users' manual weighing of samples, and further improves the automation degree of the microfluidic detection system and the user experience.
  • sample stage 70 may be fixed, and the microfluidic pesticide residue detection chip 10 is set to be movable, which can also facilitate the sampling operation.
  • the microfluidic detection system 1 further includes a buffer bottle 36 and a buffer drive device 30 .
  • Buffer bottle 36 is used to contain buffer.
  • the buffer drive device 30 is in communication with the buffer bottle 36 to controllably drive the buffer in the buffer bottle 36 into the sample cup 2 placed on the sample stage 70 , so that the buffer is mixed with the sample in the sample cup 2 .
  • Generate sample fluid Specifically, the buffer bottle 36 is communicated with the buffer driving device 30 through the introduction pipe 32 .
  • the lead-out tube 31 of the buffer driving device 30 extends to the sample stage 70 .
  • the sample to be tested is a solid sample, and a buffer solution is needed to dissolve the substance to be detected on the solid sample into it to form a sample solution; or, the sample is a liquid sample, but the concentration is too high, and a buffer solution needs to be used.
  • the sample solution is produced after dilution.
  • the samples to be tested are usually solid food scraps such as epidermis and leaves.
  • the samples need to be placed in a buffer solution, and the residual pesticides on the samples are dissolved in the buffer solution to form a sample solution.
  • the buffer driving device 30 may be a peristaltic pump, a diaphragm pump or other suitable type of driving device.
  • the peristaltic pump or diaphragm pump may generate large vibration in its radial direction during operation.
  • the radial outer side of the peristaltic pump or diaphragm pump may be provided with an elastic vibration damping member 35 .
  • the elastic damping member 35 can be sleeved on the outside of the buffer driving device 30 , and supported in the housing 80 by the clamping action of the bracket 87 and the fixing block 89 , and the fixing block 89 can be fixed on the support plate 86 .
  • the microfluidic detection system 1 further includes a housing 80 , and the chip mounting mechanism 51 , the sample liquid driving device 40 , the detection mechanism 20 and at least a part of the microfluidic biochip 10 are all disposed in the housing 80 .
  • the housing 80 is provided with a first structural connector 81 for connecting with the box or door of the refrigerator 100 , and a first structural connector 81 for forming an electrical connection between the microfluidic detection system 1 and the electronic control device of the refrigerator 100 .
  • the electrical connector 82 allows the microfluidic detection system 1 to be mounted to the cabinet or door of the refrigerator 100 as a whole.
  • the housing 80 is formed with an operation table 83 which is open toward the front side thereof, and the sample table 70 is at least partially located in the operation table 83 , so that it is convenient for the user to place the sample cup 2 in the operation table 83 , take out the sample cup 2 , etc. operate.
  • the operating table 83 may be provided with a water receiving box 88 under the sample table 70 to receive the liquid that may be dripped, so as to avoid contamination of the operating table 83 .
  • the microfluidic detection system 1 further includes a circuit board 53 , a display device 56 and a switch button 57 .
  • the circuit board 53 is disposed in the casing 80 and is electrically connected to the first electrical connector 82 on the casing 80 . connect.
  • the electrical components of the microfluidic detection system 1 (for example, the lifting mechanism 60 , the buffer driving device 30 , the sample liquid driving device 40 , the detection mechanism 20 , the display device 56 , the switch buttons 57 , etc.) are directly or indirectly connected to the circuit board 53 . electrical connection.
  • the display device 56 is disposed on the front side of the casing 80 and is electrically connected to the circuit board 53 for displaying the detection result of the detection mechanism 20 .
  • the switch button 57 is disposed on the front side of the housing 80 and is electrically connected to the circuit board 53 to enable and/or disable the detection function of the microfluidic detection system 1 . That is, the user can start, pause or stop the detection function of the microfluidic detection system 1 by operating the switch button 57 .
  • the housing 80 may include a rear shell 84 on the rear side and a front panel 85 attached to the front side of the rear shell 84 . After the rear case 84 and the front panel 85 are assembled, a cavity is defined between the two.
  • a support plate 86 and a bracket 87 are also provided in the accommodating cavity of the housing 80 .
  • the support plate 86 is fixedly connected with the rear case 84 , and at least part of the structure of the lift mechanism 60 (eg, the immovable part of the lift mechanism) and the buffer driving device 30 are fixed on the support plate 86 .
  • the bracket 87 is fixedly connected to the front side of the support plate 86 , and both the microfluidic biochip 10 and the sample liquid driving device 40 are directly or indirectly supported on the bracket 87 . Therefore, the lifting mechanism 60 , the buffer solution driving device 30 , the microfluidic biochip 10 and the sample liquid driving device 40 can be stably supported in the accommodation formed between the rear case 84 and the front panel 85 through the support plate 86 and the bracket 87 . intracavity.
  • the elevating mechanism 60 may be disposed on the lateral side of the sample stage 70
  • the buffer driving device 30 may be disposed on one side of the microfluidic biochip 10 in the lateral direction, above the elevating mechanism 60 .
  • the sample liquid driving device 40 is located on the other side of the microfluidic biochip 10 in the lateral direction
  • the buffer bottle 36 is located on the side of the sample liquid driving device 40 away from the microfluidic biochip 10 .
  • the microfluidic biochip 10 , the sample stage 70 , the lifting mechanism 60 , the buffer solution driving device 30 , the sample solution driving device 40 and the buffer solution bottle 36 after such a layout make full use of the vertical and horizontal dimensions of each module
  • Features make the layout of each module more compact and reduce the occupied space as much as possible.
  • the modules are only arranged side by side in the vertical direction and the lateral direction, and the thickness of the microfluidic detection system 1 in the front and rear directions is reduced as much as possible, so that it is more suitable for being integrated on a refrigerator.
  • a transversely extending baffle 861 may also be provided between the buffer driving device 30 and the lifting mechanism 60 to prevent the possible leakage of the buffer liquid driving device 30 from falling on the lifting mechanism 60 to prevent the normal operation of the lifting mechanism 60 . Operation has an impact.
  • the partition plate 861 may be fixed on the support plate 86 .
  • the microfluidic detection device 1 is disposed on the door body 300 , which is not only convenient to operate, but also does not occupy the original storage space in the box body 200 and does not affect the storage capacity of the refrigerator 100 itself. make an impact.
  • FIG. 12 is a schematic structural exploded view of a door body according to an embodiment of the present invention.
  • the front side of the door body 300 has a hollow window 301 , and the sample stage 70 of the microfluidic detection system 1 is exposed to the front side of the door body 300 through the hollow window 301 , so that the door body 300 can be opened without having to open the door body 300 .
  • the user is allowed to place the sample cup on the sample table 70, avoiding the problem of serious leakage of cold energy caused by opening the door 300 for each test, ensuring the thermal insulation performance of the refrigerator 100 and saving energy consumption.
  • the door body 300 may include a panel 302 for forming its front, a door lining 303 for forming its rear, and a foam insulation layer (not shown in the figure) disposed between the panel 302 and the door lining 303 ), the hollow window 301 is opened on the panel 302 .
  • a pre-embedded box 304 is pre-embedded between the panel 302 and the door liner 303 before the foam insulation layer is formed, and the microfluidic detection system 1 is arranged in the pre-embedded box 304 .
  • the pre-embedded box 304 is pre-set between the panel 302 and the door liner 303 before the door body 300 is foamed, so as to be reserved between the panel 302 and the door liner 303 for installing microfluidic detection System 1 space.
  • the pre-embedded box 304 is attached to the rear surface of the panel 302 , and the front side of the pre-embedded box 304 is open and faces the hollow window 301 , so as to allow the microfluidic detection system 1 to pass through the hollow window 301 from front to back.
  • the installation into the pre-embedded box 304 improves the convenience of installation of the microfluidic detection system 1 .
  • the pre-embedded box 304 may be provided with a second structural connector 305 that is matched with the first structural connector 81 and a second electrical connector 306 that is electrically connected to the first electrical connector 82.
  • the second electrical connector 306 is electrically connected to the electronic control device of the refrigerator 100 . Therefore, by arranging corresponding structural connectors and electrical connectors on the embedded box 304 and the housing 80, the microfluidic detection system 1 is installed on the door body 300 as a whole, so as to realize the structure and circuit. The connection between the entire microfluidic detection system 1 and the refrigerator 100 . Therefore, not only the assembly process of the microfluidic detection system 1 is simplified, but also the disassembly or maintenance of the microfluidic detection system 1 is facilitated.
  • the refrigerator 100 of the present application is a refrigerator in a broad sense, which includes not only a refrigerator in a narrow sense, but also a storage device with refrigeration, freezing or other storage functions, such as a refrigerator, a freezer, and the like.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Combustion & Propulsion (AREA)
  • General Engineering & Computer Science (AREA)
  • Thermal Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

一种冰箱，其包括用于对样本液的预设检测参数进行定性或定量检测的微流控检测系统，微流控检测系统包括：微流控生物芯片，具有进样口、连通端口、以及形成在其内部的检测池，进样口、检测池、以及连通端口之间通过微流道依次连通；芯片安装机构，用于安装微流控生物芯片；样本液驱动装置，与连通端口密封地连通，以促使与进样口接触的样本液流入微流道并经微流道流向检测池；以及检测机构，用于对检测池进行检测，以获取样本液的预设检测参数。由此，不但能够顺利地执行进样、检测操作，而且还可通过进样的精确控制确保检测结果的准确性。同时还简化了微流控检测系统的结构，避免其占用冰箱上过多空间。

Description

冰箱 技术领域
本发明涉及冷藏冷冻技术，特别是涉及一种冰箱。
背景技术
随着人们生活水平的提高，日常生活中通常需要对食用的一些食材的农残、病毒、营养元素或其他方面进行检测，以定性或定量地获取食材的状况。例如，由于农药滥用问题，我们日常买到的果蔬和农副产品有可能出现农残含量超标的问题，如果不能及时发现这些食品的农残含量超标问题，人体摄入后会造成极大危害。再如，目前提倡的母乳喂养，只有在母乳具有正常营养价值的情况下才是对婴儿最好的喂养，然而在乳母生病、吃药、手术或其他情况下可能导致其分泌的乳汁中的营养元素含量降低甚至产生病毒，从而影响婴儿的生长发育和健康。
然而，现有的检测系统一般是独立存在的，占用空间、不便于收纳，将检测装置收纳起来后又会忘记使用，或者嫌麻烦不拿出来使用。为此，现有技术中存在将用于农残检测的检测系统集成在冰箱上的方案。现有的集成在冰箱上的农残检测系统通过食材挥发的气体或从食材上流下的冷凝水进行检测，不但需要布置气体收集或液体收集装置导致结构非常复杂、占用空间较大影响用户正常的储物空间，而且检测准确度很低，从而在很大程度上影响了用户的使用体验。
发明内容
本发明的一个目的旨在克服现有技术的至少一个缺陷，提供一种集成有结构简单的微流控检测系统的冰箱。
本发明的一个进一步的目的是提高用户更换微流控生物芯片的操作便利性。
本发明的另一个进一步的目的是减弱微流控检测系统的振动、降低其运行噪音、提高其散热效率。
为了实现上述目的，本发明提供一种冰箱，包括用于对样本液的预设检测参数进行定性或定量检测的微流控检测系统，所述微流控检测系统包括：
微流控生物芯片，具有进样口、连通端口、以及形成在其内部的检测池，所述进样口、所述检测池、以及所述连通端口之间通过微流道依次连通；
芯片安装机构，用于安装所述微流控生物芯片；
样本液驱动装置，与所述连通端口密封地连通，以促使与所述进样口接触的样本液流入所述微流道并经所述微流道流向所述检测池；以及
检测机构，用于对所述检测池进行检测，以获取所述样本液的预设检测参数。
可选地，所述样本液驱动装置通过密封对接机构与所述连通端口之间形成流体密封连接；且
所述微流控生物芯片的连通端口处固定地设有一朝外凸出延伸的插接针，所述插接针的内部流道与所述连通端口密封地连通，且所述插接针插入所述密封对接机构的内部并与所述密封对接机构形成流体密封连接，所述密封对接机构与所述样本液驱动装置流体密封连接，从而使得所述样本液驱动装置与所述连通端口密封地连通。
可选地，所述插接针的延伸末端的端面呈连续且平滑的半球面，所述插接针的用于与所述密封对接机构流体连通的针孔形成在所述插接针的处于所述密封对接机构内部的区段的周向侧面。
可选地，所述微流控检测系统还包括：
芯片退出机构，用于可操作地解除所述芯片安装机构对所述微流控生物芯片的支撑作用，以释放所述微流控生物芯片。
可选地，所述芯片安装机构包括相对设置的两个弹性夹爪，以向处于两个所述弹性夹爪之间的所述微流控生物芯片施加相向作用力，从而使得所述微流控生物芯片夹持在两个所述弹性夹爪之间；且
所述芯片退出机构设置成可操作地向两个所述弹性夹爪施加方向相反的作用力，以促使两个所述弹性夹爪朝相互背离的方向产生弹性变形，从而解除两个所述弹性夹爪对所述微流控生物芯片的夹持作用。
可选地，所述微流控检测系统还包括：
样品台，用于放置样本杯，所述样本杯用于盛放样本液；且
所述样品台设置成受控地或可操作地运动，以通过所述样品台将放置于其上的样本杯输送至允许所述样本杯中的所述样本液与所述微流控生物芯片的进样口相接触的位置。
可选地，所述微流控生物芯片设置在所述样品台的上方，所述进样口位于所述微流控生物芯片的底部；且
所述微流控检测系统还包括用于驱动所述样品台上下移动的升降机构，以使得所述样品台在允许置于所述样品台上的样本杯中的样本液与所述进样口接触的检测位置和处于所述检测位置下方预设距离的初始位置之间切换。
可选地，所述微流控检测系统还包括：
缓冲液瓶，用于容装缓冲液；以及
缓冲液驱动装置，与所述缓冲液瓶连通，以受控地驱动所述缓冲液瓶内的缓冲液进入放置在所述样品台上的样本杯，从而使所述缓冲液与所述样本杯中的样本混合后产生样本液。
可选地，所述样本液驱动装置邻近地设置在所述微流控生物芯片在横向上的旁侧，且包括悬空设置的驱动电机。
可选地，所述微流控检测系统还包括：
样品台，用于放置样本杯，所述样本杯用于盛放样本液；其中
所述样品台包括用于在支撑样本杯的支撑台和设置于所述支撑台的振荡器，所述振荡器用于在所述支撑台上放置所述样本杯后对所述样本杯进行振荡，以使得所述样本杯中的缓冲液和样本充分混合后产生所述样本液。
可选地，所述微流控检测系统还包括：
壳体，所述芯片安装机构、所述样本液驱动装置、所述检测机构和所述微流控生物芯片的至少一部分均设置在所述壳体内；且
所述壳体上设有用于与所述冰箱的箱体或门体相连的结构连接件、以及用于在所述微流控检测系统与所述冰箱的电控装置之间形成电连接的电连接件，以允许所述微流控检测系统作为一个整体安装至所述冰箱的箱体或门体。
可选地，所述冰箱还包括：
箱体，其内限定有用于储存物品的储物空间；以及
门体，与所述箱体相连，且用于打开和/或关闭所述储物空间；其中
所述微流控检测装置设置于所述门体上。
本发明的冰箱包括微流控检测系统，且微流控检测系统包括用于提供检测条件和检测环境的微流控生物芯片、用于安装微流控生物芯片的芯片安装机构、用于精确地控制样本液流入微流控生物芯片的样本液驱动装置、以及用于执行检测操作的检测机构。这四个模块的结构和功能两方面的组合不但能够顺利地执行进样、检测操作，而且还可通过进样的精确控制确保检测结果的准确性。在此基础上，每个模块的结构都非常简单，简化了微流控检测系统的结构，避免其占用冰箱上过多空间。
进一步地，微流控检测系统还包括芯片退出机构，用户可通过操作芯片退出机构解除芯片安装机构与微流控生物芯片的支撑作用，从而释放微流控生物芯片，用户即可轻易地取出微流控生物芯片或者微流控生物芯片可在其自身的重力作用下掉落，简化了用户的操作过程，提高了用户更换微流控生物芯片的操作便利性。
进一步地，样本液驱动装置具有驱动电机，驱动电机悬空设置，不与其他结构接触，避免了驱动电机运行时产生的振动传递至微流控生物芯片或其他结构，从而减弱了整个微流控检测系统的振动、降低了其运行噪音。并且，由于驱动电机使用频率较高，发热量较大，因此，驱动电机悬空设置还增大了其周围的空间大小，有利于散热。
根据下文结合附图对本发明具体实施例的详细描述，本领域技术人员将会更加明了本发明的上述以及其他目的、优点和特征。
附图说明
后文将参照附图以示例性而非限制性的方式详细描述本发明的一些具体实施例。附图中相同的附图标记标示了相同或类似的部件或部分。本领域技术人员应该理解，这些附图未必是按比例绘制的。附图中：
图1是根据本发明一个实施例的冰箱的示意性结构图；
图2是根据本发明一个实施例的微流控检测系统的示意性结构图；
图3是根据本发明一个实施例的微流控检测系统的示意性结构分解图；
图4是根据本发明一个实施例的微流控检测系统内部结构的示意性结构图；
图5是根据本发明一个实施例的微流控检测系统内部结构的示意性结构分解图；
图6是根据本发明一个实施例的微流控生物芯片的示意性剖视图；
图7是根据本发明一个实施例的样本液驱动装置和微流控生物芯片及二者相接结构的示意性剖视图；
图8是根据本发明另一个实施例的密封对接机构和微流控生物芯片的示意性剖视分解图；
图9是图8中部分A的示意性放大图；
图10是根据本发明一个实施例的微流控生物芯片、芯片安装机构和芯片退出机构的示意性结构图；
图11是根据本发明一个实施例的升降机构和样品台处于分解状态的示意性结构图；
图12是根据本发明一个实施例的门体的示意性结构分解图。
具体实施方式
本发明提供一种冰箱。图1是根据本发明一个实施例的冰箱的示意性结构图。参见图1，本发明涉及的冰箱100包括箱体200和门体300。箱体200内限定有用于储存物品的储物空间，门体300与箱体200相连，且用于打开和/或关闭其储物空间。
特别地，冰箱100还包括微流控检测系统1，微流控检测系统1用于对样本液的预设检测参数进行定性或定量检测，该预设检测参数例如可以为用于表示农残量是否超标和/或农残量的具体数值的农残参数、用于表示营养元素是否达标和/或营养元素具体含量的营养参数、用于表示特定有害物质(例如特定病毒)是否超标和/或具体含量的特定物质参数等等。
图2是根据本发明一个实施例的微流控检测系统的示意性结构图，图3是根据本发明一个实施例的微流控检测系统的示意性结构分解图，图4是根据本发明一个实施例的微流控检测系统内部结构的示意性结构图，图5是根据本发明一个实施例的微流控检测系统内部结构的示意性结构分解图。为了便于理解，图1至图5中还示出了样本杯2。
参见图2至图5，微流控检测系统1可包括微流控生物芯片10、芯片安装机构51、样本液驱动装置40和检测机构20。本领域技术人员可以理解的是，当微流控检测系统用于检测的预设检测参数不同时，其所使用的微流控生物芯片10和检测机构20的具体选择可能也有所不同。例如，当微流控检测系统用于农残检测时，其具有的微流控生物芯片10可以是能够为农残液提供检测条件的微流控农残检测芯片，其具有的检测机构20可以是能够对农残液的农残参数进行检测的农残检测机构。
图6是根据本发明一个实施例的微流控生物芯片的示意性剖视图，微流控生物芯片10具有进样口111、连通端口112、以及形成在其内部的检测池121，进样口111、检测池121、以及连通端口112之间通过微流道14依次连通，从而允许与进样口111接触的样本液流入微流道14并经微流道14流入检测池121。本发明所涉及的微流道14意指过流面积在预设尺寸范围内的细微流道或毛细流道，以使其具有合适的保持其内液体的能力。进样口111和连通端口112可形成在微流控生物芯片10的端部。进一步地，进样口111和连通端口112优选形成在微流控生物芯片10的不同端部。
芯片安装机构51用于安装微流控生物芯片10，以为微流控生物芯片10提供支撑。
样本液驱动装置40与连通端口112密封地连通，以促使与进样口111接触的样本液流入微流道14并经微流道14流向检测池121，从而对进入检测池121的样本液的量和流动速度进行精确地控制。
检测机构20用于对检测池121进行检测，以获取样本液的预设检测参数。具体地，检测池121内可预先设有检测试剂，也可通过人为地或自动地向检测池121内添加检测试剂，以在检测池121内的样本液和其内的检测试剂反应后通过检测机构20对检测池121进行检测。
本发明的冰箱100包括微流控检测系统1，且微流控检测系统1包括用于提供检测条件和检测环境的微流控生物芯片10、用于安装微流控生物芯片10的芯片安装机构51、用于精确地控制样本液流入微流控生物芯片10的样本液驱动装置40、以及用于执行检测操作的检测机构20。这四个模块的结构和功能两方面的组合不但能够顺利地执行进样、检测操作，而且还可通过进样的精确控制确保检测结果的准确性。在此基础上，每个模 块的结构都非常简单，简化了微流控检测系统1的结构，避免其占用冰箱100上过多空间。
在一个具体的实施例中，当检测机构20为用于对农残液的农残参数进行检测的农残检测机构时，可使用酶抑制率法对样本液的农残是否超标进行快速的定性检测。此时，微流控生物芯片10还包括形成在其内部的反应池122，反应池122位于进样口111、检测池121、以及连通端口112依次连通形成的主通道上，并连通在进样口111和检测池121之间，以使得样本液先与反应池122内的反应试剂反应后再流入检测池121。反应池122与进样口111之间、以及反应池122与检测池121之间均通过微通道14连通。用于农残检测的反应试剂和检测试剂可以分别为酶试剂和显色剂。反应池122用于供样本液和其内的酶试剂反应，与酶试剂反应后的样本液流入检测池121，与检测池121内的显色剂进行反应。检测机构20可以选择为光电检测机构，其可以包括分别设置在微流控生物芯片10的两个相对的侧部并均与检测池121正对的光源21和光敏元件22，光源21发出的光照射至检测池121，透过检测池121的光导入光敏元件22，从而利于通过光敏元件22接收到的光强信号判断检测池121内的吸光度变化，进而计算农残抑制率。进一步地，检测机构20还包括用于向检测池121提供热量的加热片24和用于控制加热片24加热功率恒定的温控器25，以使得检测池121内的样本液和检测试剂充分、快速地反应。
在一些实施例中，样本液驱动装置40可邻近地设置在微流控生物芯片10在横向上的旁侧，由此，既保证了微流控生物芯片10和样本液驱动装置40之间结构布局的紧凑性，又可以在微流控生物芯片10内的液体产生泄漏或外排时使液体顺着微流控生物芯片10向下流动或滴落，而不会接触样本液驱动装置40，从而避免了对样本液驱动装置40产生不良影响。
进一步地，样本液驱动装置40可包括悬空设置的驱动电机41。也就是说，驱动电机41仅通过其顶部与支撑结构相连，不与其他结构接触，避免了驱动电机41运行时产生的振动传递至微流控生物芯片10或其他结构，不但可防止对微流控生物芯片10或其他结构的稳定性或性能产生不良影响，而且还减弱了整个微流控检测系统1的振动、降低了其运行噪音。并且，由于驱动电机41使用频率较高，发热量较大，因此，驱动电机41悬空设置还增大了其周围的空间大小，有利于散热。
具体地，样本液驱动装置40可以通过向外抽空气的方式在主通道内形成负压，从而使得与进样口111接触的样本液在负压作用下进入主通道。图7是根据本发明一个实施例的样本液驱动装置和微流控生物芯片及二者相接结构的示意性剖视图。在一些实施例中，样本液驱动装置40可以为微型注射泵，且还包括竖向延伸的注射器42、丝杆43、滑块44和活塞45。注射器42固定在一支架87上，且注射器42的顶部通过连接管路46与微流控生物芯片10顶部的连通端口112密封连通。丝杆43沿竖向延伸，且与驱动电机41相连，以在驱动电机41的驱动下转动。滑块44穿设在丝杆43上，并与丝杆43螺纹连接，以随丝杆43的转动沿丝杆43上下平移。活塞45设置于注射器42的内部，且与滑块44固定连接，以在滑块44的带动下沿上下方向平移，从而在其向下平移时使得主通道内产生负压进而促使与进样口111接触的样本液流入微流道并经微流道流入检测池121、在其向上平移时促使主通道内的样本液向进样口111流动。
样本液驱动装置40还可进一步包括位置传感器，用于配合驱动电机41控制活塞45向上平移和/或向下平移的位移量，进而实现进样的精确控制。同时，还可以通过对活塞 45向上平移和向下平移的位移量进行实时监测，使其执行细微的推抽操作，从而在确保样本液不会经连通端口112流出的前提下对进入主通道内的样本液进行方向相逆的推液和吸液动作，以促使检测池121内的样本液与其内的检测试剂更加均匀地混合或更加充分地反应，提高了检测结果的精准性。
在一些实施例中，样本液驱动装置40通过密封对接机构90与连通端口112之间形成流体密封连接。参见图7，密封对接机构90可包括密封连接件91和弹性施压件92。密封连接件91连接在微流控生物芯片10和样本液驱动装置40之间，且其内形成有贯穿密封连接件91的连接通道。弹性施压件92用于向密封连接件91施加弹性作用力，以使得密封连接件91同时与样本液驱动装置40和微流控生物芯片10密封对接，从而使得样本液驱动装置40和微流控生物芯片10的连通端口112通过密封连接件91内部的连接通道密封地连通。由此，可通过弹性施压件92向密封连接件91施加弹性作用力，以促使密封连接件91始终保持同时与样本液驱动装置40和微流控生物芯片10紧密地密封对接的状态，避免了采用其他对接机构可能导致长时间使用后产生松动、断折等问题，从而确保了样本液驱动装置40和微流控生物芯片10的连通端口112之间长期的、可靠的流体密封连通关系、提高了二者之间的密封效果。
在一些实施例中，样本液驱动装置40与连通端口112之间通过连接管路46连通。连通端口112可形成在微流控生物芯片10的顶部，样本液驱动装置40可邻近地设置在微流控生物芯片10在横向上的旁侧，以避免微流控生物芯片10可能产生的漏液对样本液驱动装置40造成不良影响。连接管路46可与样本液驱动装置40的顶部连通，以跨接在样本液驱动装置40与微流控生物芯片10之间。
进一步地，密封连接件91可包括用于与微流控生物芯片10直接对接的第一连接块912和设置在第一连接块912的背离微流控生物芯片10所在侧的第二连接块913。第一连接块912与第二连接块913之间、以及第二连接块913与连接管路46之间均以插接的方式形成密封连接。
在一些实施例中，弹性施压件92可以为弹簧，弹簧的一端抵接于一固定设置的端板513，另一端抵接于密封连接件91，端板513和微流控生物芯片10分别处于密封连接件91的相对的两侧。具体地，在微流控生物芯片10处于安装状态下，弹簧处于被压缩的状态，从而产生用于促使密封连接件91具有朝向微流控生物芯片10移动趋势的弹性作用力。弹性施压件92的数量可以为两个或两个以上，以增加作用在微流控生物芯片10的弹性作用力大小，并使得微流控生物芯片10受到的弹性作用力更加平衡，避免产生倾斜，进一步提高了密封连接的效果。
进一步地，密封对接机构90还包括导杆93，弹簧套设在导杆93上，以避免弹簧移位。导杆93的一端与密封连接件91固定连接，另一端在微流控生物芯片10与密封连接件91密封对接后与一霍尔开关94接触，从而促使霍尔开关94产生用于表示微流控生物芯片10安装到位的触发信号，以便提示用户，避免了微流控生物芯片10过度安装导致结构损坏，同时还提高了用户的使用体验。
为了提高密封对接机构90与微流控生物芯片10之间的密封性能，本申请还提供了另一种实施例的密封对接机构90和微流控生物芯片10。图8是根据本发明另一个实施例的密封对接机构和微流控生物芯片的示意性剖视分解图，图9是图8中部分A的示意性放大图。其中，图8中隐去了密封对接机构的弹性施压件、导杆等结构。在另一些实施 例中，本发明的微流控生物芯片10的连通端口112处还特别地固定连接有朝外凸出延伸的插接针15，插接针15的内部流道151与连通端口112密封地连通，且插接针15插入密封对接机构90的内部并与密封对接机构90形成流体密封连接。也就是说，密封对接机构90同时与样本液驱动装置40和插接针15流体密封连接，实现了样本液驱动装置40和微流控生物芯片10的连通端口112之间良好的密封连通关系。
具体地，插接针15可插接在密封对接机构90的密封连接件91中。图8和图9所示实施例中的密封连接件91与图7中所示实施例的密封连接件91的结构稍有不同。图8和图9所示实施例中的密封连接件91的第一连接块912的底部可设有供插接针15插入的通孔9121，通孔9121与插接针15之间可通过抵接接触或挤压接触的方式密封配合。插接针15上可开设有用于将其内部流道151与密封对接机构90的内部流体连接的针孔152，针孔152开设在插接针15的处于密封对接机构90内部的区段上，也就是使得插接针15的针孔152处于密封对接机构90的内部，既确保了二者之间畅通良好的流体连通关系，又在很大程度上提高了二者之间的密封性能，避免二者之间的连接处产生漏气、漏液等问题。
进一步地，为了避免密封对接机构90在频繁地插拔插接针15后受到损坏，可以对密封对接机构90的结构强度进行提高，然而这对密封对接机构90的材料要求较高，并且即使采用结构强度较高的材料，在有限次地插拔插接针15后，密封对接机构90仍然会受到结构损坏。为此，本申请的申请人从另一个角度出发，对插接针15的结构进行改进。参见图9，插接针15的延伸末端的端面153呈连续且平滑的半球面，插接针15的用于与密封对接机构90流体连通的针孔152形成在插接针15的处于密封对接机构90内部的区段的周向侧面154。由此，在将设有插接针15的微流控生物芯片10与密封对接机构90密封对接时，插接针15与密封对接机构90的接触面是平滑的球面，减小了插接针15与密封对接机构90之间的摩擦，因此不会划伤或刺破密封对接机构90，确保了密封对接机构90长期保持较好的密封对接功能，提高了微流控检测系统1的使用寿命，同时，还降低了对密封对接机构90的结构强度的要求。
需要说明的是，插接针15的延伸末端意指插接针15伸入密封对接机构90内部的一端。进一步地，针孔152可形成在插接针15邻近其延伸末端的区段的周向侧面，由此，即使插接针15插入密封对接机构90的区段不长，仍然可以确保插接针15与密封对接机构90之间的流体连通关系。
更进一步地，参见图8和图9，插接针15经连通端口112插接在微流控生物芯片10的内部，插接针15伸入微流控生物芯片10内部的始端敞开，以便于与微流道14连通，从而与连通端口112连通。插接针15与连通端口112的配合界面处可通过密封胶16密封，以增强插接针15与微流控生物芯片10之间的密封性能。
在一些替代性实施例中，插接针15也可以与微流控生物芯片10一体成型。
在一些实施例中，微流控检测系统1还包括芯片退出机构52，用于可操作地解除芯片安装机构51对微流控生物芯片10的支撑作用，以释放微流控生物芯片10。由此，用户可通过操作芯片退出机构52解除芯片安装机构51与微流控生物芯片10的支撑作用，从而释放微流控生物芯片10，用户即可轻易地取出微流控生物芯片10或者微流控生物芯片10可在其自身的重力作用下掉落，简化了用户的操作过程，提高了用户更换微流控生物芯片10的操作便利性。
进一步地，芯片退出机构52可裸露于冰箱100的外部，便于用户实施芯片退出操作，无论微流控检测系统1自身的结构布局以及其集成于冰箱后的整体结构布局有多紧凑，都不会影响微流控生物芯片10的拆卸操作，提高了用户的使用体验。
图10是根据本发明一个实施例的微流控生物芯片、芯片安装机构和芯片退出机构的示意性结构图。在一些实施例中，芯片安装机构51可包括相对设置的两个弹性夹爪511，以向处于两个弹性夹爪511之间的微流控生物芯片10施加相向作用力，从而使得微流控生物芯片10夹持在两个弹性夹爪511之间。并且，芯片退出机构52设置成可操作地向两个弹性夹爪511施加方向相反的作用力，以促使两个弹性夹爪511朝相互背离的方向产生弹性变形，从而解除两个弹性夹爪511对微流控生物芯片10的夹持作用。
具体地，芯片退出机构52可包括悬垂在微流控生物芯片10一侧的悬臂按键521和由悬臂按键521的朝向微流控生物芯片10的内侧向逐渐靠近微流控生物芯片10的方向凸出延伸的抵接块522，抵接块522同时与两个弹性夹爪511的相向设置的内侧抵接，以在悬臂按键521受到朝向微流控生物芯片10的作用力时通过抵接块522向两个弹性夹爪511的内侧施加朝外的作用力，从而使得两个弹性夹爪511朝相互背离的外侧方向弹性变形。也就是说，当需要拆卸微流控生物芯片10时，用户只需要按压悬臂按键521即可解除两个弹性夹爪511对微流控生物芯片10的夹持作用，从而释放微流控生物芯片10，操作非常简便，且芯片退出机构52的结构非常简单，设计非常巧妙。
抵接块的分别与两个弹性夹爪511的内侧相抵的两个相对的侧面沿逐渐靠近微流控生物芯片10的方向相向倾斜，以确保悬臂按键521按压操作的顺畅性，避免出现卡顿等现象。具体地，抵接块大致可呈等腰梯形，等腰梯形的下底与悬臂按键521相连，等腰梯形的两个腰分别与两个弹性夹爪511的内侧相抵接。
在一些实施例中，微流控检测系统1还包括样品台70，样品台70用于放置样本杯2，样本杯2用于盛放样本液。并且，样品台70设置成受控地或可操作地运动，以通过样品台70将放置于其上的样本杯2输送至允许样本杯2中的样本液与微流控生物芯片10的进样口111相接触的位置。由此，实现了微流控生物芯片10的加样。用户只需要将样本杯2放置在样品台70上，或者，在将样本杯2放置在样品台70后再将样品台70移动至与微流控生物芯片10的进样口111相接触的位置即可，加样操作非常便捷，省时省力。并且，本申请通过将样品台70设置成可动的，省去了样本液输送泵、输送管路、采样针等复杂的结构，使得微流控检测系统1的结构非常简单，从而避免占用冰箱过多空间。
进一步地，微流控生物芯片10可设置在样品台70的上方，进样口111位于微流控生物芯片10的底部以便于进样口111与放置在样品台70上的样本杯2中的样本液接触。微流控检测系统1还包括用于驱动样品台70上下移动的升降机构60，以使得样品台70在允许置于样品台70上的样本杯2中的样本液与进样口111接触的检测位置和处于检测位置下方预设距离的初始位置之间切换。也就是说，样品台70可以通过升级机构60自动升降，进一步简化了用户的操作，提高了微流控检测系统的自动化程度。
图11是根据本发明一个实施例的升降机构和样品台处于分解状态的示意性结构图。在一些实施例中，升降机构60可包括升降电机61、传动丝杆62和螺母63。升降电机61用于输出驱动力。传动丝杆62沿竖直方向设置，且与升降电机61的输出轴相连，以在升降电机61的驱动下转动。螺母63穿设在传动丝杆62上，并与传动丝杆62螺纹连接，以随传动丝杆62的转动沿传动丝杆62上下移动。样品台70与螺母63固定连接，以通 过螺母63带动样品台70上下移动。
进一步地，升降机构60还包括滑轨64和滑块65。滑轨64与传动丝杆62相平行地设置在传动丝杆62的旁侧，滑块65可移动地设置在滑轨64上，样品台70与滑块65固定连接，以通过滑轨64和滑块65的配合引导样品台70上下移动。具体地，样品台70在驱动模块的作用下沿上下方向移动时带动滑块65同步移动，滑块65被限制在滑轨64上，滑轨64对滑块65的移动具有引导和限位的作用，从而间接地对样品台70产生引导和限位作用，避免了样品台70在移动过程中产生偏移或卡顿，提高了样品台70运动的平稳性。具体地，样品台70可包括穿设在传动丝杆62中并与螺母63固定相连的水平连接板74以及垂直于水平连接板74向上延伸的竖直连接板75，竖直连接板75与滑块65固定连接。
在一些实施例中，升降机构60还包括限位开关66，限位开关66邻近传动丝杆62的上部设置，以在样品台70向上移动至触碰到限位开关66时促使升降电机61停止运行。并且，限位开关66的位置设置成当升降电机61在限位开关66的触发下停止运行时使得样品台70处于其检测位置。升降电机61不运行时可使样品台70保持在其检测位置。本申请通过限位开关66来定位样品台70的检测位置，定位精准，可避免样品台70超出其检测位置继续移动导致样品台70、微流控生物芯片10等结构损坏的问题。
在一些实施例中，样品台70可包括支撑台71和振荡器72。支撑台71用于支撑样本杯2。具体地，支撑台71可以为水平放置的支撑板，支撑板上可设置用于供样本杯2的底部放置于其内的凹槽，以在样品台70的移动过程中避免样本杯2倾倒或晃动，提高了样本杯2放置的稳固性。支撑台71与水平连接板74固定连接。
振荡器72设置于支撑台71上，用于在支撑台71上放置样本杯2后对样本杯2进行振荡，以使得样本杯2中的缓冲液和样本充分混合后产生样本液，从而使得样本上的待检测物质充分地溶解到缓冲液中得到合适浓度的样本液。缓冲液可以通过手动添加的方式预置在样本杯2中，也可以在样本杯2放置在样品台70后通过驱动装置自动地输送至样本杯2。
在一些实施例中，样品台70还包括称重传感器73，称重传感器73设置于支撑台71的下方，以用于测称样本杯2中样本的重量，从而允许缓冲液驱动装置30将与样本重量相匹配的预设量的缓冲液输送至样本杯2。通常情况下，家庭用户对样本的提取是比较随意的，比如随意撕下一小片菜叶，因此，为了保证测量结果的准确性，输入样本杯2中的缓冲液的量需要与样本的量相匹配，这样才能够产生合适浓度的样本液。本申请通过置于支撑台71下方的称重传感器73可自动地、精确地获得样本的重量，从而自动控制缓冲液驱动装置30向样本杯2中输入匹配量的缓冲液，既保证了测量结果的准确性，又避免了用户手动测称样本导致使用不便、操作繁琐、误差较大等诸多问题，进一步提高了微流控检测系统的自动化程度和用户的使用体验。
需要说明的是，在一些替代性实施例中，样品台70可以为固定的，微流控农残检测芯片10设置成可动的，同样能够便于取样操作。
在一些实施例中，微流控检测系统1还包括缓冲液瓶36和缓冲液驱动装置30。缓冲液瓶36用于容装缓冲液。缓冲液驱动装置30与缓冲液瓶36连通，以受控地驱动缓冲液瓶36内的缓冲液进入放置在样品台70上的样本杯2，从而使缓冲液与样本杯2中的样本混合后产生样本液。具体地，缓冲液瓶36与缓冲液驱动装置30之间通过引入管32连通。 缓冲液驱动装置30的引出管31延伸至样本台70。这主要是针对被检测的样本为固态样本，需要利用缓冲液将固态样本上的待检测物质溶解到其中从而形成样本液；或者，样本为液态样本，但是浓度过高，需要利用缓冲液对其进行稀释后产生样本液。例如，在进行农残检测时，被检测的样本通常为表皮、叶片等固体的食材残片，需要将样本置于缓冲液中，样本上的残留农药溶解到缓冲液中，从而形成样本液。
具体地，缓冲液驱动装置30可以为蠕动泵、隔膜泵或其他合适类型的驱动装置。蠕动泵或隔膜泵在运行时会在其径向上产生较大的振动，为了避免该振动传递至微流控生物芯片10，蠕动泵或隔膜泵的径向外侧可设有弹性减振件35。弹性减振部件35可套设在缓冲液驱动装置30的外部，并通过支架87和固定块89的夹持作用支撑在壳体80内，固定块89可固定在支撑板86上。
在一些实施例中，微流控检测系统1还包括壳体80，芯片安装机构51、样本液驱动装置40、检测机构20和微流控生物芯片10的至少一部分均设置在壳体80内。壳体80上设有用于与冰箱100的箱体或门体相连的第一结构连接件81、以及用于在微流控检测系统1与冰箱100的电控装置之间形成电连接的第一电连接件82，以允许微流控检测系统1作为一个整体安装至冰箱100的箱体或门体。
进一步地，壳体80上形成有朝向其前侧敞开的操作台83，样品台70至少部分地位于操作台83中，从而便于用户在操作台83中实施放置样本杯2、取出样本杯2等操作。操作台83中可设有处于样品台70下方的接水盒88，以承接可能滴落的液体，避免污染操作台83。
在一些实施例中，微流控检测系统1还包括电路板53、显示装置56和开关按键57，电路板53设置于壳体80内，且与壳体80上的第一电连接件82电连接。微流控检测系统1的用电部件(例如升降机构60、缓冲液驱动装置30、样本液驱动装置40、检测机构20、显示装置56、开关按键57等)均直接或间接地与电路板53电连接。显示装置56设置在壳体80的前侧，且与电路板53电连接，以用于显示检测机构20的检测结果。开关按键57设置在壳体80的前侧，且与电路板53电连接，以用于启动和/或关闭微流控检测系统1的检测功能。也就是说，用户可通过操作开关按键57启动、暂停或停止微流控检测系统1的检测功能。
在一些实施例中，壳体80可包括处于后侧的后壳84和连接在后壳84前侧的前面板85。后壳84与前面板85组装后在二者之间限定处容纳腔。并且，壳体80的容纳腔内还设有支撑板86和支架87。支撑板86与后壳84固定连接，升降机构60的至少部分结构(如升降机构的不可动部分)和缓冲液驱动装置30均固定在支撑板86上。支架87固定连接在支撑板86的前侧，微流控生物芯片10和样本液驱动装置40均直接或间接地支撑在支架87上。由此，可通过支撑板86与支架87将升降机构60、缓冲液驱动装置30、微流控生物芯片10和样本液驱动装置40稳固地支撑在后壳84与前面板85之间形成的容纳腔内。
在一些实施例中，升降机构60可设置在样品台70在横向上的旁侧，缓冲液驱动装置30可设置在微流控生物芯片10在横向上的一侧，并位于升降机构60的上方，样本液驱动装置40位于微流控生物芯片10在横向上的另一侧，缓冲液瓶36位于样本液驱动装置40的背离微流控生物芯片10的一侧。这样布局后的微流控生物芯片10、样品台70、升降机构60、缓冲液驱动装置30、样本液驱动装置40和缓冲液瓶36充分地利用了各个 模块在竖直方向和横向上的尺寸特征，使得各个模块的布局更加紧凑，尽可能地减小占用空间。并且，各个模块之间仅在竖直方向上和横向上并排设置，尽可能地缩小了微流控检测系统1在前后方向上的厚度，以使其更加适宜于集成在冰箱上。
进一步地，缓冲液驱动装置30和升降机构60之间还可设有横向延伸的隔板861，以避免缓冲液驱动装置30可能产生的漏液滴落在升降机构60上对升降机构60的正常运行产生影响。隔板861可固定在支撑板86上。
在一些实施例中，微流控检测装置1设置于门体300上，不但操作起来比较方便，而且还不会占用箱体200内原有的储物空间，不会对冰箱100本身的储物能力产生影响。
图12是根据本发明一个实施例的门体的示意性结构分解图。在一些实施例中，门体300的前侧具有镂空窗口301，微流控检测系统1的样品台70经镂空窗口301暴露于门体300的前侧，由此，可在不必打开门体300的情况下允许用户向样品台70放置样品杯，避免每次检测都要打开门体300导致冷量泄露严重的问题，确保了冰箱100的保温性能，节省了能耗。
具体地，门体300可包括用于形成其前部的面板302、用于形成其后部的门衬303以及设置在面板302和门衬303之间的发泡保温层(图中未示出)，镂空窗口301开设在面板302上。面板302和门衬303之间在形成发泡保温层之前预埋有一预埋盒304，微流控检测系统1设置在预埋盒304内。也就是说，预埋盒304是在门体300发泡之前预先设置在面板302和门衬303之间的，用于在面板302和门衬303之间预留出用于安装微流控检测系统1的空间。
进一步地，预埋盒304贴设于面板302的后向表面，且预埋盒304的前侧敞开，并正对镂空窗口301，以允许微流控检测系统1经镂空窗口301从前往后地安装至预埋盒304内，提高了微流控检测系统1安装的便利性。
具体地，预埋盒304上可设有与第一结构连接件81匹配连接的第二结构连接件305和与第一电连接件82电连接的第二电连接件306，第二电连接件306与冰箱100的电控装置电连接。由此，通过在预埋盒304和壳体80上设置相应的结构连接件和电连接件使得微流控检测系统1作为一个整体安装至门体300上，从而在结构和电路两个方面实现整个微流控检测系统1与冰箱100之间的连接。由此，不但简化了微流控检测系统1的装配过程，而且便于微流控检测系统1的拆卸或维修。
本申请的冰箱100为广义上的冰箱，其不但包括通常所说的狭义上的冰箱，而且还包括具有冷藏、冷冻或其他储物功能的储物装置，例如，冷藏箱、冷柜等等。
本领域技术人员还应理解，本发明实施例中所称的“上”、“下”、“前”、“后”、“顶”、“底”等用于表示方位或位置关系的用语是以冰箱100的实际使用状态为基准而言的，这些用语仅是为了便于描述和理解本发明的技术方案，而不是指示或暗示所指的装置或不见必须具有特定的方位、以特定的方位构造和操作，因此不能理解为对本发明的限制。
至此，本领域技术人员应认识到，虽然本文已详尽示出和描述了本发明的多个示例性实施例，但是，在不脱离本发明精神和范围的情况下，仍可根据本发明公开的内容直接确定或推导出符合本发明原理的许多其他变型或修改。因此，本发明的范围应被理解和认定为覆盖了所有这些其他变型或修改。

Claims (12)

  1. 一种冰箱，包括用于对样本液的预设检测参数进行定性或定量检测的微流控检测系统，所述微流控检测系统包括：
    微流控生物芯片，具有进样口、连通端口、以及形成在其内部的检测池，所述进样口、所述检测池、以及所述连通端口之间通过微流道依次连通；
    芯片安装机构，用于安装所述微流控生物芯片；
    样本液驱动装置，与所述连通端口密封地连通，以促使与所述进样口接触的样本液流入所述微流道并经所述微流道流向所述检测池；以及
    检测机构，用于对所述检测池进行检测，以获取所述样本液的预设检测参数。
  2. 根据权利要求1所述的冰箱，其中，
    所述样本液驱动装置通过密封对接机构与所述连通端口之间形成流体密封连接；且所述微流控生物芯片的连通端口处固定地设有一朝外凸出延伸的插接针，所述插接针的内部流道与所述连通端口密封地连通，且所述插接针插入所述密封对接机构的内部并与所述密封对接机构形成流体密封连接，所述密封对接机构与所述样本液驱动装置流体密封连接，从而使得所述样本液驱动装置与所述连通端口密封地连通。
  3. 根据权利要求2所述的冰箱，其中，
    所述插接针的延伸末端的端面呈连续且平滑的半球面，所述插接针的用于与所述密封对接机构流体连通的针孔形成在所述插接针的处于所述密封对接机构内部的区段的周向侧面。
  4. 根据权利要求1所述的冰箱，其中，所述微流控检测系统还包括：
    芯片退出机构，用于可操作地解除所述芯片安装机构对所述微流控生物芯片的支撑作用，以释放所述微流控生物芯片。
  5. 根据权利要求4所述的冰箱，其中，
    所述芯片安装机构包括相对设置的两个弹性夹爪，以向处于两个所述弹性夹爪之间的所述微流控生物芯片施加相向作用力，从而使得所述微流控生物芯片夹持在两个所述弹性夹爪之间；且
    所述芯片退出机构设置成可操作地向两个所述弹性夹爪施加方向相反的作用力，以促使两个所述弹性夹爪朝相互背离的方向产生弹性变形，从而解除两个所述弹性夹爪对所述微流控生物芯片的夹持作用。
  6. 根据权利要求1所述的冰箱，其中，所述微流控检测系统还包括：
    样品台，用于放置样本杯，所述样本杯用于盛放样本液；且
    所述样品台设置成受控地或可操作地运动，以通过所述样品台将放置于其上的样本杯输送至允许所述样本杯中的所述样本液与所述微流控生物芯片的进样口相接触的位置。
  7. 根据权利要求6所述的冰箱，其中，
    所述微流控生物芯片设置在所述样品台的上方，所述进样口位于所述微流控生物芯片的底部；且
    所述微流控检测系统还包括用于驱动所述样品台上下移动的升降机构，以使得所述样品台在允许置于所述样品台上的样本杯中的样本液与所述进样口接触的检测位置和处于所述检测位置下方预设距离的初始位置之间切换。
  8. 根据权利要求6所述的冰箱，其中，所述微流控检测系统还包括：
    缓冲液瓶，用于容装缓冲液；以及
    缓冲液驱动装置，与所述缓冲液瓶连通，以受控地驱动所述缓冲液瓶内的缓冲液进入放置在所述样品台上的样本杯，从而使所述缓冲液与所述样本杯中的样本混合后产生样本液。
  9. 根据权利要求1所述的冰箱，其中，
    所述样本液驱动装置邻近地设置在所述微流控生物芯片在横向上的旁侧，且包括悬空设置的驱动电机。
  10. 根据权利要求1所述的冰箱，其中，所述微流控检测系统还包括：
    样品台，用于放置样本杯，所述样本杯用于盛放样本液；其中
    所述样品台包括用于在支撑样本杯的支撑台和设置于所述支撑台的振荡器，所述振荡器用于在所述支撑台上放置所述样本杯后对所述样本杯进行振荡，以使得所述样本杯中的缓冲液和样本充分混合后产生所述样本液。
  11. 根据权利要求1所述的冰箱，其中，所述微流控检测系统还包括：
    壳体，所述芯片安装机构、所述样本液驱动装置、所述检测机构和所述微流控生物芯片的至少一部分均设置在所述壳体内；且
    所述壳体上设有用于与所述冰箱的箱体或门体相连的结构连接件、以及用于在所述微流控检测系统与所述冰箱的电控装置之间形成电连接的电连接件，以允许所述微流控检测系统作为一个整体安装至所述冰箱的箱体或门体。
  12. 根据权利要求1所述的冰箱，还包括：
    箱体，其内限定有用于储存物品的储物空间；以及
    门体，与所述箱体相连，且用于打开和/或关闭所述储物空间；其中
    所述微流控检测装置设置于所述门体上。
PCT/CN2021/117714 2020-09-27 2021-09-10 冰箱 Ceased WO2022062919A1 (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US18/246,486 US11883819B2 (en) 2020-09-27 2021-09-10 Refrigerator
EP21871291.7A EP4206569B1 (en) 2020-09-27 2021-09-10 Refrigerator

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202011029619.7A CN114279136B (zh) 2020-09-27 2020-09-27 冰箱
CN202011029619.7 2020-09-27

Publications (1)

Publication Number Publication Date
WO2022062919A1 true WO2022062919A1 (zh) 2022-03-31

Family

ID=80844937

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/117714 Ceased WO2022062919A1 (zh) 2020-09-27 2021-09-10 冰箱

Country Status (4)

Country Link
US (1) US11883819B2 (zh)
EP (1) EP4206569B1 (zh)
CN (1) CN114279136B (zh)
WO (1) WO2022062919A1 (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114280313A (zh) * 2020-09-27 2022-04-05 青岛海尔电冰箱有限公司 用于冰箱的微流控检测系统及冰箱

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120011458A (ko) * 2010-07-29 2012-02-08 엘지전자 주식회사 냉장고
CN103196887A (zh) * 2013-03-27 2013-07-10 重庆绿色智能技术研究院 一种有机农药检测用高通量微流控装置及其水样检测方法
CN204165319U (zh) * 2014-09-22 2015-02-18 四川长虹电器股份有限公司 果蔬农药残留检测冰箱
CN109655421A (zh) * 2019-02-14 2019-04-19 杭州霆科生物科技有限公司 一种全自动农药残留检测系统
CN214039111U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 冰箱

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5230866A (en) * 1991-03-01 1993-07-27 Biotrack, Inc. Capillary stop-flow junction having improved stability against accidental fluid flow
US5498392A (en) * 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
US7524464B2 (en) * 2003-09-26 2009-04-28 Ahn Chong H Smart disposable plastic lab-on-a-chip for point-of-care testing
US9056291B2 (en) * 2005-11-30 2015-06-16 Micronics, Inc. Microfluidic reactor system
CN106016925B (zh) * 2016-05-30 2018-08-10 青岛海尔股份有限公司 用于冰箱的门体及其制备方法、冰箱
CN108801929A (zh) * 2018-06-26 2018-11-13 杭州霆科生物科技有限公司 一种能够检测食品安全的智能家居
CN109174220B (zh) * 2018-10-16 2023-11-14 湖南乐准智芯生物科技有限公司 一种生物芯片及芯片控制方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20120011458A (ko) * 2010-07-29 2012-02-08 엘지전자 주식회사 냉장고
CN103196887A (zh) * 2013-03-27 2013-07-10 重庆绿色智能技术研究院 一种有机农药检测用高通量微流控装置及其水样检测方法
CN204165319U (zh) * 2014-09-22 2015-02-18 四川长虹电器股份有限公司 果蔬农药残留检测冰箱
CN109655421A (zh) * 2019-02-14 2019-04-19 杭州霆科生物科技有限公司 一种全自动农药残留检测系统
CN214039111U (zh) * 2020-09-27 2021-08-24 青岛海尔电冰箱有限公司 冰箱

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP4206569A4 *

Also Published As

Publication number Publication date
EP4206569A4 (en) 2024-02-14
EP4206569A1 (en) 2023-07-05
US20230271184A1 (en) 2023-08-31
CN114279136B (zh) 2025-01-10
US11883819B2 (en) 2024-01-30
CN114279136A (zh) 2022-04-05
EP4206569B1 (en) 2025-01-29

Similar Documents

Publication Publication Date Title
CN214039111U (zh) 冰箱
CN214039171U (zh) 用于冰箱的微流控检测系统及冰箱
WO2022062909A1 (zh) 冰箱
CN214039172U (zh) 用于冰箱的农残检测系统及冰箱
CN214039173U (zh) 用于冰箱的微流控检测系统及冰箱
CN115248326B (zh) Poct血细胞分析仪及其使用方法
WO2022062905A1 (zh) 用于冰箱的微流控检测系统及冰箱
CN114324904B (zh) 用于冰箱的微流控检测系统及冰箱
WO2022062919A1 (zh) 冰箱
CN114324909B (zh) 用于冰箱的微流控检测系统及冰箱
WO2023093383A1 (zh) 用于冰箱的微流控检测系统及冰箱
CN114324913A (zh) 用于冰箱的微流控检测系统及冰箱
CN114324903A (zh) 用于冰箱的微流控检测系统及其控制方法、冰箱
WO2023045438A1 (zh) 用于冰箱的微流控检测系统及冰箱
CN115248331A (zh) Poct血细胞分析仪及其使用方法、移液器
CN115248325A (zh) Poct血细胞分析仪及其使用方法
CN115248180A (zh) Poct血细胞分析仪及其使用方法
CN115248323A (zh) 检测座及poct血细胞分析仪
CN115248328A (zh) Poct血细胞分析仪及其使用方法
CN216525868U (zh) Poct血细胞分析仪
CN216525867U (zh) Poct血细胞分析仪
CN216387080U (zh) Poct血细胞分析仪、试剂盒
CN115248321B (zh) Poct血细胞分析仪及其使用方法
CN115248322B (zh) Poct血细胞分析仪及其使用方法
WO2022226746A1 (zh) Poct血细胞分析仪及其使用方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21871291

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase

Ref document number: 2021871291

Country of ref document: EP

Effective date: 20230330

NENP Non-entry into the national phase

Ref country code: DE