WO2023063468A1 - 인공육 및 인공육 제조 방법 - Google Patents
인공육 및 인공육 제조 방법 Download PDFInfo
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- WO2023063468A1 WO2023063468A1 PCT/KR2021/015993 KR2021015993W WO2023063468A1 WO 2023063468 A1 WO2023063468 A1 WO 2023063468A1 KR 2021015993 W KR2021015993 W KR 2021015993W WO 2023063468 A1 WO2023063468 A1 WO 2023063468A1
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- extruded
- meat
- fiber
- fibers
- artificial meat
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
- A23J3/225—Texturised simulated foods with high protein content
- A23J3/227—Meat-like textured foods
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
- A23J3/26—Working-up of proteins for foodstuffs by texturising using extrusion or expansion
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/20—Synthetic spices, flavouring agents or condiments
- A23L27/26—Meat flavours
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P30/00—Shaping or working of foodstuffs characterised by the process or apparatus
- A23P30/20—Extruding
- A23P30/25—Co-extrusion of different foodstuffs
Definitions
- the present invention relates to artificial meat and a method for producing artificial meat.
- artificial meat refers to food manufactured to taste similar to meat. Artificial meat replaces part of the demand for livestock farming as part of a stable food supply, contributing to mitigating environmental pollution caused by livestock farming. In addition, some of the artificial meat is produced based on non-animal ingredients, contributing to meeting the needs of vegetarians. For this reason, the demand for artificial meat is increasing day by day, and a method for mass-producing it is needed.
- artificial meat by conventional methods does not have the meat texture and marbling of real meat, so there is a slight difference from the taste and texture of real meat.
- the problem to be solved by the present invention is to provide a method for producing artificial meat with excellent mass productivity.
- the problem to be solved by the present invention is to provide artificial meat and a manufacturing method similar to real meat in taste and texture.
- the step of forming a first extruded raw material in which the raw material realizing the taste of lean meat is solubilized, the first extruded raw material is extruded Obtaining at least one first extruded fiber having a length along the extrusion direction, forming a second extrusion source material in which a raw material embodying the taste of fat is solubilized, and extruding the second extrusion source material in the extrusion direction obtaining at least one second extruded fiber having a length according to and obtaining artificial meat in which at least one first extruded fiber and at least one second extruded fiber are connected along the length direction; .
- the first extrusion power source material may be extruded through at least one first nozzle.
- the second extrusion power source material may be extruded through at least one second nozzle.
- the artificial meat is formed on a cross section cut in a direction crossing the lengthwise direction of the first extruded fibers. and a pattern formed of the second extruded fibers.
- the method may further include cutting the artificial meat in a direction crossing the longitudinal direction to obtain at least one piece of artificial meat having marbling according to the pattern.
- a crosslinking agent may be sprayed on the first extruded power source passing through the first nozzle to obtain the crosslinked first extruded fibers.
- a crosslinking agent may be sprayed into the second extrusion power source passing through the second nozzle to obtain the crosslinked second extruded fibers.
- the first extruded raw material passing through the first nozzle may be immersed in a crosslinking agent to obtain the crosslinked first extruded fibers.
- the second extrusion source material passing through the second nozzle may be immersed in a crosslinking agent to obtain the crosslinked second extruded fibers.
- the first extruded power source material passing through the first nozzle is exposed to an environment for gelation, and the gelated first extruded fiber may be obtained.
- the second extruded power source material passed through the second nozzle is exposed to an environment for gelation, and the gelled second extruded fibers may be obtained.
- an adhesive is applied to at least one of the first extruded fiber and the second extruded fiber so that the at least one first extruded fiber and the at least one second extruded fiber are formed in the longitudinal direction.
- the first extruded fiber and the second extruded fiber are immersed in an adhesive, so that at least one first extruded fiber and at least one second extruded fiber are connected along the longitudinal direction.
- cells are cultured in a state in which at least one first extruded fiber and at least one second extruded fiber are arranged along the longitudinal direction, and at least one of the at least one extruded fiber is obtained by the cultured cells.
- a first extruded fiber and at least one of the second extruded fibers may be connected.
- At least one first extruded fiber, at least one second extruded fiber, and at least one third extruded fiber may be connected along the longitudinal direction.
- the raw material for realizing the taste of lean meat is processed into a fiber form, the step of obtaining edible fiber, and folding at least one of the edible fiber
- a step of obtaining a fat unit in which a raw material embodying the taste of fat is gelled or cross-linked may be further included.
- At least one edible fiber and at least one fat unit may be combined to form artificial meat in which marbling of real meat is realized in a pattern formed by the combination.
- the fat unit may be obtained by being processed into a fiber form.
- the artificial meat includes an edible fiber structure in which at least one of the edible fibers is folded or a plurality of the edible fibers are connected in a bundle form, and at least one of the fat units is folded or a plurality of the edible fibers are formed. It may be formed by a combination of fat structures in which fat units are connected in a bundle form.
- marbling according to the part may be implemented by changing a pattern resulting from a combination of the edible fiber and the fat unit according to the part of the real meat to be copied.
- the method may further include encapsulating cells of a specific animal in a raw material that embodies the taste of the lean meat so that the artificial meat is similar to the meat of the specific animal.
- Artificial meat according to the first embodiment of the present invention for solving the above problems is formed by extruding a first extruded raw material in which the first raw material of artificial meat is solubilized, and at least one first raw material having a length along the extrusion direction 1 extruded fiber and a second extruded power source material obtained by solubilizing the second raw material of the artificial meat are formed by extrusion, and include at least one second extruded fiber having a length along the extrusion direction, and at least one of the first extruded A fiber and at least one second extruded fiber may be formed by being connected along the length direction.
- Artificial meat according to a second embodiment of the present invention for solving the above problems may include at least one edible fiber formed by processing a raw material for realizing the taste of lean meat into a fiber form.
- At least a portion of the artificial meat may be formed by folding at least one of the edible fibers or connecting a plurality of the edible fibers in a bundle form so that the artificial meat includes a structure similar to a bundle structure of muscle fibers in real meat. .
- the artificial meat may further include a fat unit in which a raw material realizing the taste of fat is gelled or crosslinked.
- marbling of real meat may be implemented in a pattern formed by a combination of at least one edible fiber and at least one fat unit.
- 1 is a schematic representation of real meat.
- FIG. 2 is a flowchart of a method for manufacturing artificial meat according to a first embodiment of the present invention.
- Figure 3 is for the case where the step of forming the extrusion power source material in which the raw material is solubilized according to the first embodiment of the present invention includes the step of forming the first extrusion power source material and the step of forming the second extrusion power source material It is a flow chart.
- Figure 4 is a flow chart for the case where the step of obtaining the extruded fibers according to the first embodiment of the present invention includes the step of obtaining the first extruded fiber and the step of obtaining the second extruded fiber.
- 5 is a schematic representation of extruding extruded fibers using the extrusion device according to the first embodiment.
- FIG. 6 is a photograph of extruded fibers extruded by the extrusion device according to the first embodiment.
- FIG. 7 is a view schematically illustrating the production of artificial meat using an extrusion device according to a second embodiment.
- FIG. 8 is a view schematically illustrating artificial meat produced by an extrusion device according to a second embodiment and artificial meat pieces obtained by cutting the artificial meat.
- FIG. 9 is a flowchart of a method for manufacturing artificial meat according to a second embodiment of the present invention.
- Figure 10 is for the case where the step of forming the extrusion power source material in which the raw material is solubilized according to the second embodiment of the present invention includes the step of forming the first extrusion power source material and the step of forming the second extrusion power source material. It is a flow chart.
- step 11 is a flow chart for a case where the step of obtaining extruded fibers according to the second embodiment of the present invention includes a step of obtaining first extruded fibers and a step of obtaining second extruded fibers.
- FIG. 12 is a flowchart of a method for manufacturing artificial meat according to a third embodiment of the present invention.
- FIG. 13 is a diagram schematically illustrating examples of fat units that may be used in the artificial meat manufacturing method according to the third embodiment of the present invention.
- FIG. 14 is a photograph of an edible fiber structure prepared according to a third embodiment of the present invention.
- 15 is a photograph of artificial meat prepared according to a third embodiment of the present invention.
- Artificial meat means artificial meat produced by humans. Artificial meat should be understood as a concept that includes cultured meat made by culturing cells and substitute meat manufactured by processing edible raw materials.
- FIG. 1 is a schematic representation of real meat. As shown in FIG. 1, actual meat can be largely divided into a lean meat portion 11 and a fat portion 12.
- the lean meat portion 11 refers to a portion formed on the basis of muscle fibers 111 in meat. As shown in FIG. 1 , the lean meat portion 11 of the actual meat 1 includes numerous muscle fibers 111 arranged in a bundle shape. The taste and texture of the lean meat part 1 is influenced by the composition, size and/or arrangement of these muscle fibers 111.
- the fat portion 12 is a portion constituting marbling in the actual meat 1.
- the content of fat (12) is different for each part. This is because areas with high motility have a relatively small amount of fat accumulation, and areas with low motility have a relatively high amount of fat accumulation. This difference in the content of the fat part 12 has a great effect on the taste of the actual meat 1. This is because meat with a higher content of the fat portion 12 generally has a softer texture and a savory taste.
- the method for manufacturing artificial meat according to the first embodiment of the present invention includes forming an extruded raw material solubilized as a raw material (S110), obtaining extruded fibers (S130), and artificial meat It may include a step of obtaining (S150) and a step of obtaining artificial meat pieces (S160).
- the artificial meat raw material is solubilized to obtain the extruded raw material in a sol and/or gel state.
- raw materials for artificial meat may be dissolved in an appropriate solvent and processed into a gel state.
- raw materials for artificial meat may be processed into a sol state using an appropriate dispersion medium.
- a raw material in a hydrogel state is formed in the step (S110) in which the extruded raw material in which the raw material is solubilized is formed.
- the raw material may be variously processed into a sol and/or gel state.
- those skilled in the art will be able to manufacture artificial meat using variously processed raw materials based on the following description.
- the raw materials used in this step are raw materials for forming a hydrogel with extrudable properties (hereinafter referred to as 'basic raw materials'), raw materials for imitating taste (hereinafter referred to as 'flavor raw materials'), and optionally additiveable raw materials. (hereinafter referred to as 'additive raw materials').
- 'basic raw materials' raw materials for imitating taste
- 'additive raw materials' optionally additiveable raw materials.
- 'additive raw materials' optionally additiveable raw materials.
- the basic raw material may be prepared with edible materials such as alginate, agarose, xanthan gum, guar gum, gum arabic, carrageenan, gelatin, and collagen.
- edible materials such as alginate, agarose, xanthan gum, guar gum, gum arabic, carrageenan, gelatin, and collagen.
- the flavor ingredient may be selected as an ingredient to mimic the taste of lean meat, an ingredient to mimic the taste of fat, an ingredient to mimic the taste of processed meat, and/or an ingredient to mimic the taste of other foods.
- other foods may be a concept that includes not only meat and meat accessories, but also edible foods such as fish, fruits, vegetables, and cooked dishes without limitation. Specific examples of flavor raw materials will be described below.
- the additive raw material may be a functional raw material that can be added as needed.
- the additive raw material may be a nutrient or a medicinal ingredient.
- At least one of the basic raw materials, flavor raw materials, and additive raw materials used to form the hydrogel may include a material that is insoluble at pH 7, such as high-molecular collagen or textured soy protein (TSP).
- TSP textured soy protein
- acidic or basic solvents may be used to work with the hydrogel. Therefore, the fabricated hydrogel may not be neutral in some cases.
- the user if the user requires neutralization of the hydrogel in the food manufacturing regulations of the country where the user intends to sell and/or manufacture, the user neutralizes the hydrogel by adding an appropriate acidic and/or basic substance to the hydrogel in this step. can be performed.
- step S130 the user obtains the extruded fibers by extruding the solubilized extrusion power source material with an extrusion device.
- the extruded fibers extruded by the extrusion device have a length along the extrusion direction.
- the extrusion device may be a device for extruding the hydrogel through an extrusion hole having a fine diameter or a fine gap.
- the extrusion device may be a device in which at least one fine extrusion hole and/or gap is formed.
- the extrusion device may be provided with a device including a plurality of nozzles or a 3D printer including a plurality of nozzles.
- the extruded fibers extruded in a hydrogel state may be crosslinked and/or gelated.
- a crosslinker used for crosslinking the extrusion power source material may be appropriately selected depending on the composition of the hydrogel material.
- the user can gel the extrusion power source material through heating/cooling and/or acid-base reaction in consideration of the composition of the extrusion power source material.
- Crosslinking to extruded fibers can be accomplished in a variety of ways.
- the user may crosslink the extruded fibers by spraying an aerosol crosslinking agent toward the extruded fibers.
- the user may cross-link extruded fibers by immersing them in a cross-linking agent in a solution state.
- the user may expose the extruded fibers to an aqueous solution and/or aerosol containing calcium ions at a sufficient concentration (1 to 200 mM).
- the user may place the extruded fibers in an aqueous solution and/or aerosol containing sufficient concentrations of genipin, glutaraldehyde, transglutaminase, and the like. can be exposed.
- artificial meat is formed by arranging a plurality of extruded fibers in a bundle shape and then connecting them to each other.
- a method of connecting the extruded fibers to each other a method using an adhesive and a method using cell culture are exemplified.
- a method of connecting extruded fibers using an adhesive is a method of connecting a plurality of extruded fibers in a bundle form using an adhesive.
- the user may connect the extruded fibers to each other by applying adhesive to the surface of the extruded fibers or dipping the extruded fibers into the adhesive.
- the method of applying the adhesive may be realized by applying a liquid adhesive to the surface of the extruded fiber or spraying and applying an aerosol adhesive to the surface of the extruded fiber.
- the method of immersing in an adhesive may be demonstrated by a method of connecting a plurality of extruded fibers in a bundle form in a state in which a plurality of extruded fibers are immersed in a bowl containing a liquid adhesive.
- extruded fibers coated with an adhesive or dipped in an adhesive may be connected in a bundle form by applying pressure to adhere to each other for 10 minutes to 300 minutes under a temperature condition of 4 degrees Celsius to 40 degrees Celsius.
- the adhesive used in this step may include an edible adhesive composed of edible ingredients and/or an edible crosslinking agent.
- the edible adhesive may be an adhesive composed of cyanoacrylate-based adhesive, polyurethane-based adhesive, fibrin glue, gelatin glue, and/or other edible ingredients.
- the edible crosslinker may be a chemical crosslinker composed of genipin, glutaraldehyde, and/or other edible ingredients.
- the method of connecting extruded fibers using cell culture is to apply a hydrogel uniformly encapsulated with cells capable of connecting extruded fibers (hereinafter referred to as 'connecting cells') to the surface of compressed fibers, or to extrude in an aqueous solution mixed with connecting cells. It can be demonstrated by immersing the fibers and then culturing the cells.
- the cells placed on the surface of the extruded fibers in the manner described above are then cultured and grown to form cell structures connecting a plurality of fiber bundles.
- Culturing of the neuronal cells may be the same as or similar to general cell and/or tissue culture conditions (hereinafter referred to as 'general culture conditions').
- connecting cells When cells with excellent extracellular matrix productivity are selected as connecting cells, the user may culture the connecting cells for about 6 hours or more under normal culture conditions to form a cell structure.
- these connecting cells may be at least one of fibroblasts, mesenchymal stem cells, myofibroblasts, and other cells actively producing extracellular matrix.
- the user may culture the connecting cells for about 12 hours or more under normal culture conditions to form a cell structure.
- the artificial meat is cut in a direction crossing the longitudinal direction.
- Artificial meat pieces may mean each piece obtained by cutting artificial meat. In particular, this step may be effective when mass-producing artificial meat is desired.
- the user can form the length of the extruded fiber to be very long in the step of obtaining the extruded fiber (S130). Thereafter, the artificial meat formed through the step of obtaining artificial meat (S150) is divided into several pieces as needed in this step.
- the user may divide the artificial meat into equal parts so that the plurality of artificial meat pieces have a certain thickness, or cut the artificial meat so that at least some of the artificial meat pieces have different thicknesses.
- artificial meat is manufactured using raw materials that simulate different tastes so that the artificial meat has a complex taste in which at least two or more flavors are mixed.
- each of the two tastes is described as a taste of lean meat and the other is a taste of fat, but the present invention is not limited thereto.
- each of the two tastes may be a taste that mimics any one of the tastes of various foods, such as fish, fruits, vegetables, and cooked dishes, as well as meat and meat accessories.
- Figure 3 is for the case where the step of forming the extrusion power source material in which the raw material is solubilized according to the first embodiment of the present invention includes the step of forming the first extrusion power source material and the step of forming the second extrusion power source material It is a flow chart.
- the first raw material constituting the artificial meat is processed into the first extruded raw material in a hydrogel state.
- the first ingredient may be an ingredient selected so that artificial meat mimics the taste of lean meat.
- the first raw material may include a flavor raw material and a basic raw material that simulates the taste of lean meat.
- the first raw material may be constituted without a basic raw material.
- the first raw material may further contain additional raw materials including medicinal ingredients, nutritional ingredients, and the like.
- the flavor raw material that simulates the taste of lean meat may be at least one of heme, hemoglobin, myoglobin, leghemoglobin, cysteine, and other raw meat flavoring raw materials.
- cysteine is an amino acid related to the Maillard reaction that occurs in meat when meat is cooked, and when included in the first raw material, there is an advantage in that manufactured artificial meat can closely mimic the flavor of cooked meat. .
- the second raw material constituting the artificial meat is processed into a second extruded raw material in a hydrogel state.
- the second ingredient may be an ingredient selected so that artificial meat mimics the taste of fat.
- the second raw material may include a flavor raw material and a basic raw material that simulates the taste of fat.
- the second raw material may be constituted without a basic raw material.
- the second raw material may further contain additional raw materials including medicinal ingredients, nutritional ingredients, and the like.
- the flavor ingredient for flavoring the fat may be at least one of lard oil, canola oil, palm oil, sunflower oil, lipids, fats and other fat flavoring ingredients.
- Figure 4 is a flow chart for the case where the step of obtaining the extruded fiber according to the first embodiment of the present invention includes the step of obtaining the first extruded fiber and the step of obtaining the second extruded fiber.
- step S131 of obtaining the first extruded fiber the first extruded power source is placed in an extrusion device and extruded to obtain the first extruded fiber.
- the extrusion device may be a device including a plurality of first nozzles through which the first extrusion power source material is extruded.
- the user extrudes the first extruded fiber using an extrusion device, and determines the length of the first extruded fiber as needed.
- the second extruded power source is placed in an extrusion device and extruded and crosslinked to obtain the second extruded fibers.
- the extrusion device may be a device including a plurality of second nozzles through which the second extrusion power source material is extruded.
- the user extrudes the second extruded fiber using an extrusion device, and determines the length of the second extruded fiber as needed.
- the artificial meat manufacturing method according to the first embodiment may further include forming a third extruded raw material in which the additive raw material is solubilized, and obtaining third extruded fibers.
- the additive raw material is hydrogelized in the same or similar manner to the first extrusion power source material or the second extrusion power source material.
- the additive raw material may be a medicinal ingredient and/or a nutrient selected in response to the needs of the consumer or prospective intake person.
- the step of obtaining the third extruded fibers may be performed in the same or similar manner to the step of obtaining the first extruded fibers (S131) and/or the step of obtaining the second extruded fibers (S132).
- the extruded fiber is obtained using the extrusion device according to the first embodiment (S130) and the artificial meat is obtained (S150).
- S130 the extrusion device according to the first embodiment
- S150 the artificial meat is obtained (S150).
- 5 is a schematic representation of extruding extruded fibers using the extrusion device according to the first embodiment.
- 6 is a photograph of extruded fibers extruded by the extrusion device according to the first embodiment.
- the extrusion device 2 is a device capable of extruding the hydrogel 30 disposed therein in a fiber form through a plurality of nozzles (not shown).
- the extrusion device 2 may include an accommodation space for accommodating the hydrogel 30 processed from raw materials therein, and a plurality of nozzles for extruding the hydrogel 30 in the accommodation space.
- the diameter of the nozzle of the extrusion device 2 may be set so that the extruded fibers have a diameter similar to that of muscle fibers.
- the nozzle of the extrusion device 2 may be formed to have an inner diameter within a range of 5 ⁇ m to 3000 ⁇ m.
- the extrusion device 2 may include about 10,000 or more nozzles, so that a large amount of extruded fibers at one time. However, this is an exemplary number and the number of nozzles included in the extrusion device 2 may vary.
- the user may form the raw material before gelation to have a complex modulus within the range of 0.01 Pa to 30 kPa.
- the user may form a hydrogel so that the hydrogel 30 has a complex elastic modulus within the range of 1 Pa to 100 MPa after gelation.
- extruded fibers 31 that are not yet crosslinked or gelled are ejected through a nozzle.
- the user may crosslink the extruded fibers 31 by spraying the crosslinking agent toward the nozzle side of the extrusion device 2 .
- the user may gelate the extruded fibers 31 by exposing them to an environment in which the extruded fibers 31 are gelled (hereinafter referred to as 'gelation environment').
- the user may cool or heat the extruded fibers, or immerse them in a water solution at a specific pH.
- the gelled or cross-linked extruded fibers 32 are dropped into a bowl 4 containing an aqueous adhesive solution at the bottom of the extrusion device 2 .
- the gelled or crosslinked extruded fibers 32 have a length proportional to the duration of extrusion and fall into the bowl 4. Therefore, it is possible for the user to obtain extruded fibers 32 of various lengths by adjusting the operating time of the extrusion device 2 .
- step S150 of obtaining artificial meat the user connects the extruded fibers 32 dropped on the bowl 4 to each other.
- the user folds or binds the extruded fibers 32 together to obtain artificial meat in the form of a bundle.
- the user may proceed with the above-described operation after disposing the first extruded raw material in the extrusion device 2.
- the user can proceed with the above-described operation after placing the second extruded raw material in the extrusion device 2.
- the user uses one extrusion device 2 to obtain the first extruded fibers (S131) and the second step.
- Step 2 (S132) of obtaining extruded fibers may be sequentially performed.
- the user can extrude the third extruded fiber obtained by extruding the third extrusion power source using the extrusion device 2. For example, the user sequentially proceeds through a step of obtaining a first extruded fiber (S131), a step of obtaining a second extruded fiber (S132), and a step of obtaining a third extruded fiber with one extrusion device (2).
- the user may simultaneously or simultaneously perform the step of obtaining the first extruded fiber (S131), the step of obtaining the second extruded fiber (S132), and the step of obtaining the third extruded fiber by different extrusion devices (2). there is.
- the diameter of the nozzle of the extrusion device 2 through which the second extruded fiber is extruded is the diameter of the nozzle of the extrusion device 2 through which the first extruded fiber is extruded. can be made larger. This may be because the first extruded fibers need to be extruded in the form of very thin fibers in order to mimic the muscle fibers of real meat, whereas the second extruded fibers mimic fat with a relatively large structure in real meat.
- the step of obtaining artificial meat (S150) may proceed by assembling the first extruded fibers and the second extruded fibers. At this time, when the third extruded fiber is extruded in the above step, the first extruded fiber, the second extruded fiber, and the third extruded fiber are assembled.
- the user arranges a plurality of first extruded fibers and a plurality of second extruded fibers (or a plurality of first extruded fibers, a plurality of second extruded fibers, and a plurality of third extruded fibers) in the longitudinal direction by the extrusion direction. After that, they can be bonded together to produce artificial meat. At this time, marbling is formed on the cross section perpendicular to the longitudinal direction of the artificial meat by the combination pattern of the first extruded fiber and the second extruded fiber.
- the user may obtain artificial meat having marbling by separately manufacturing the lean meat part and the fat part and then assembling them.
- the user forms an edible fiber structure by folding at least one first extruded fiber or connecting a plurality of first extruded fibers in a bundle form.
- the user forms the fat structure by folding at least one second extruded fiber or connecting a plurality of second extruded fibers in a bundle form.
- the user may prepare artificial meat with marbling by assembling at least one edible fiber structure and at least one fat structure.
- extruded fibers are obtained using the extrusion device according to the second embodiment (S130), artificial meat is obtained (S150), and artificial meat
- S130 artificial meat is obtained
- S150 artificial meat
- S160 artificial meat
- FIGS. 7 and 8 The process of obtaining the piece (S160) will be described.
- 7 is a view schematically illustrating the production of artificial meat using an extrusion device according to a second embodiment.
- 8 is a view schematically illustrating artificial meat produced by an extrusion device according to a second embodiment and artificial meat pieces obtained by cutting the artificial meat.
- the extrusion device 7 is provided so as to simultaneously extrude the first extruded fibers 511 and the second extruded fibers 512 .
- the extrusion device 7 separately accommodates the first extrusion power source and the second extrusion power source, and a plurality of nozzles each selectively extrude either the first extrusion power source or the second extrusion power source.
- the extrusion device 7 may include a first extrusion power source and a second extrusion power source separately accommodating and accommodating, and a valve for selectively connecting the accommodating portion and a plurality of nozzles, respectively.
- the inner diameter of the nozzle may be the same as or similar to that of the nozzle of the extrusion device 2, a detailed description thereof will be omitted.
- the step of obtaining the first extruded fibers (S131) and the step of obtaining the second extruded fibers (S132) may be performed simultaneously.
- a nozzle extruding the first extrusion power source material is referred to as a first nozzle 71
- a nozzle extruding the second extrusion power source material is referred to as a second nozzle 72.
- the nozzle of the extrusion device 7 is the first nozzle 71 or the second extrusion power source according to the user setting. It can be 2 nozzles 72.
- the extruding device 7 may include a crosslinking agent spraying device 81, an adhesive spraying device 82, and a mold (not shown) to automate the production of artificial meat.
- the crosslinking agent spraying device 81 is a device for spraying the crosslinking agent 811 toward the discharge ports of the plurality of nozzles. Due to this, the extruded fiber passing through the nozzle is exposed to the crosslinking agent and crosslinked in the process of descending by the force of extrusion.
- the artificial meat is obtained (S150) by first extruding fibers 511 and
- the adhesive 812 may be sprayed onto the second extruded fiber 522 by the adhesive spraying device 82 and proceed.
- the adhesive spraying device 82 is a device for spraying adhesive on the crosslinked extruded fibers.
- the extruded fibers to which the adhesive is sprayed may descend and enter a close contact mold (not shown).
- the close frame is formed so that the diameter of the outlet is smaller than that of the inlet, so the first extruded fibers 511 and the second extruded fibers 512 to which the adhesive is applied naturally pass through the close frame in the direction of the arrow shown in FIG.
- the artificial meat 5 may be formed by being adhered to each other and adhered to each other.
- the artificial meat 5 thus formed has a shape similar to that of FIG. 8 .
- the artificial meat 5 is formed with a lean meat portion 51 in which first extruded fibers 511 are concentrated and a fat portion 52 in which second extruded fibers 512 are densely formed.
- a cross section of artificial meat 5 cut in a direction crossing the longitudinal direction is marbling by a combination pattern of first extruded fibers 511 and second extruded fibers 512. This will be formed
- the user Since the marbling is formed by the combination pattern of the first extruded fiber 511 and the second extruded fiber 512, the user has a nozzle 71 through which the first extrusion power source material is extruded and a nozzle through which the second extrusion power source material is extruded.
- the shape of the marbling can be changed by setting the arrangement of (72) differently.
- the user can adjust the fat content of the artificial meat 5 by adjusting the ratio of the nozzle 71 through which the first extrusion power source material is extruded and the nozzle 72 through which the second extrusion power source material is extruded.
- the step of obtaining artificial meat pieces is performed by cutting the artificial meat 5 in a direction perpendicular to the longitudinal direction.
- the user cuts the artificial meat 5 several times along the longitudinal direction to produce a plurality of artificial meat pieces 6 .
- the length of the artificial meat 5 is approximately several meters or more than tens of meters, and by cutting the artificial meat 5, tens to thousands of artificial meat pieces 6 may be produced at a time.
- the extrusion device 7 according to the second embodiment crosslinks the raw material by spraying the crosslinking agent 811, but the present invention is not limited thereto.
- the extrusion device 7 may have a section that creates a gelation environment.
- the extrusion device 7 may cool (or heat) the extruded fibers by immersing them in an aqueous solution having a specific temperature or less (or more) for a preset time.
- the extrusion device 7 may cool (or heat) the extruded fibers by conveying them through a tray below (or above) a certain temperature.
- the nozzle of the extrusion device 7 is an example of selectively extruding any one of the first extruded fibers 511 and the second extruded fibers 512, but is not limited thereto.
- a plurality of raw materials are independently accommodated in the extrusion device 7, and the nozzle may selectively extrude any one of the received raw materials according to a user's setting.
- the first raw material, the second raw material, and the additive raw material may be each independently accommodated in the extrusion device 7 .
- the user can manufacture artificial meat by setting the number and positional arrangement of each of the first nozzle extruding the first raw material, the second nozzle extruding the second raw material, and the third nozzle extruding the additive raw material among the plurality of nozzles. there is.
- the user may set the number of third nozzles so that the third extruded fibers are less than (or more than) a predetermined ratio in the cross section of the artificial meat 5 to adjust the content of medicinal and nutritional ingredients.
- the user can set the raw material extruded by each nozzle so that the main taste of the artificial meat 5 is based on the first raw material and the second raw material.
- the user may set the raw material extruded by each nozzle such that the third extruded fiber is positioned between the first extruded fibers and/or the second extruded fibers.
- FIGS. 9 to 11 a method for manufacturing artificial meat according to a second embodiment of the present invention and artificial meat manufactured through the method will be described with reference to FIGS. 9 to 11 .
- the same reference numerals are used for parts similar to those of the first embodiment, and descriptions of parts common to the first embodiment are omitted.
- the artificial meat manufacturing method according to the second embodiment includes a step of forming an extruded raw material in which the raw material is solubilized (S210), a step of encapsulating cells in the extruded raw material (S220), and extruded fibers may include obtaining (S230), culturing cells (S240), obtaining artificial meat (S250), and obtaining artificial meat pieces (S260).
- the step of forming an extruded raw material in which the raw material is solubilized (S210), the step of obtaining extruded fibers (S230), the step of obtaining artificial meat (S250), and the step of obtaining artificial meat pieces (S260) are
- the extruded raw material in which the raw material is solubilized is formed (S110)
- the extruded fiber is obtained (S130)
- the artificial meat is obtained (S150)
- the artificial meat piece is obtained (S160) and Since each can be performed identically or similarly, the differences will be mainly described below.
- the user may form the raw material before gelation to have a complex modulus within the range of 0.01 Pa to 300 Pa. .
- the user may form the hydrogel so that the gel has a complex modulus within the range of 1 Pa to 300 kPa after gelation.
- neutralization of the hydrogel may be performed in order to encapsulate the cells in the hydrogel after the step (S210) in which the extruded power source material in which the raw material is solubilized is formed (S210). This is because cells can die if cells are encapsulated in a hydrogel that is not in a neutral state.
- the cells to be cultured are encapsulated in the hydrogel formed in the step of forming the extrusion power source material in which the raw material is solubilized (S210).
- Cells to be encapsulated can be broadly classified into cells cultured to realize the taste for a specific meat (hereinafter referred to as 'taste-creating cells') and the aforementioned connecting cells. Description of the connecting cells is omitted since it has been described above.
- Taste-enhancing cells may be animal cells selected in consideration of the type and/or region of meat to be produced. Actual meat tastes different due to factors such as the type of cells constituting it and the ratio of red muscle fibers to white muscle fibers.
- the user can enclose the cells of the specific animal in the hydrogel while proceeding with this step.
- the user may enclose the cells of an animal so that the texture, color, texture, and taste of the artificial meat are similar to that of a specific animal.
- the user can adjust the ratio of cells corresponding to white muscle fibers and cells corresponding to red muscle fibers to be encapsulated in the gel so that the artificial meat simulates the taste of meat for a specific part of a specific animal.
- taste embodying cells are cells for a specific part of a specific animal, muscle cells (myocyte), muscle satellite cells (satellte cells), muscle stem cells (muscle stem cells), fibroblasts (fibroblasts), endothelial cells (vascular cells) cell), chondrocyte, bone cell (oesteocyte), bone marrow/adipose tissue/nasal cavity-derived mesenchymal stem cell derived from bone marrow/adipose tissue/nasal cavity, and the like.
- the gel may be extruded within a pressure range to prevent damage to cells encapsulated in the gel during the extrusion process.
- the pressure at which the extrusion device extrudes the gel may be set to 200 kPa or less.
- the cells encapsulated in the extruded fibers are cultured according to the culture conditions of the encapsulated cells.
- cells can be cultured by exposing the extruded fibers under the general culture conditions described above for a sufficient period of time.
- the connecting cells are encapsulated in the hydrogel in the step of encapsulating the cells in the extruded power source material (S220), the step of culturing the cells (S240) and the step of obtaining the artificial meat (S250) may be performed simultaneously.
- the user may perform the step of arranging a plurality of extruded fibers in a bundle form and then culturing the cells while pressing them with a frame for compressing them (S240). At this time, a plurality of extruded fibers are connected to each other by the connective cells cultured over time to obtain artificial meat.
- FIG. 10 shows the step of forming an extrusion power source material in which the raw material is solubilized according to the second embodiment of the present invention, the step of forming the first extrusion power source material and the second step of forming the first extrusion power source material. 2 It is a flow chart for the case including the step of forming the extruded power source material. 11 is a flowchart for a case where the step of obtaining extruded fibers according to the second embodiment of the present invention includes a step of obtaining first extruded fibers and a step of obtaining second extruded fibers.
- the step of forming the first extrusion power source material (S211) and the step of forming the second extrusion power source material (S212) are the steps of forming the first extrusion power source material of the first embodiment described above (S111) and the second extrusion power source material. It may be performed similarly to the step (S112) in which is formed.
- the first extrusion power source material and / or the second extrusion power source material Cells are encapsulated in the extruded power source material.
- the first extruded fiber is obtained (S231) and the second extruded fiber is obtained (S232), the first extruded fiber of the above-described first embodiment is obtained (S231) and the second extruded fiber is obtained It may be performed similarly to step S232.
- the step of obtaining the first extruded fiber (S231) and the step of obtaining the second extruded fiber (S232) may have a more limited extrusion pressure range than the first embodiment so that cells are not damaged, and artificial meat is immediately generated. Instead, the step of culturing the cells proceeds.
- the artificial meat manufactured by the artificial meat manufacturing method according to the second embodiment also simulates the marbling of real meat. Since the description has been described above, it is omitted.
- FIGS. 12 to 15 For convenience of description, similar reference numerals are used for parts similar to those of the above-described embodiments, and descriptions of parts common to the above-described embodiments are omitted.
- 12 is a flowchart of a method for manufacturing artificial meat according to a third embodiment of the present invention.
- 13 is a diagram schematically illustrating examples of fat units that can be used in the artificial meat manufacturing method according to the third embodiment of the present invention.
- 14 is a photograph of an edible fiber structure manufactured according to a third embodiment of the present invention.
- 15 is a photograph of artificial meat manufactured according to the third embodiment of the present invention.
- the method for manufacturing artificial meat according to the third embodiment of the present invention includes gelling raw materials (S310), encapsulating cells (S320), and obtaining edible fibers (S331). , Obtaining fat units (S332), culturing cells (S340), and forming artificial meat (S350).
- the first extrusion power source material obtained by processing the first raw material into a hydrogel state identically or similarly to the above-described embodiments, and the second extrusion power source material obtained by processing the second raw material into a hydrogel state is formed
- taste-enhancing cells or connective cells may be encapsulated in the first extruded raw material and/or the second extruded raw material, similarly to the second embodiment.
- step S331 of obtaining edible fiber the user prepares edible fiber by processing the first extruded raw material into a fine fiber form. Users can process edible fibers to have diameters ranging from 5 ⁇ m to 3000 ⁇ m.
- the method of manufacturing edible fiber is not limited to the extrusion method.
- the first extruded power source material may be first applied thinly and crosslinked, and then divided into fine fibers.
- the first extruded raw material crosslinked in the form of a thin plate may be divided into fine fibers or processed into fine fibers by entering a device similar to a noodle making machine.
- edible fibers similar to fine fibers may be obtained by accommodating the first extruded raw material in a gel state in a pre-prepared molding mold and then exposed to a crosslinking agent.
- the user prepares the fat unit by processing the second extruded raw material into an arbitrary shape.
- the fat unit 513 is manufactured according to the marbling pattern of the artificial meat to be manufactured in order to shorten the process time. Doing so can be efficient.
- a fat unit 513a in the form of fine fibers or a fat unit 513b in the form of fibers is used.
- the fibrous fat unit 513b may be a fat unit formed to have a larger diameter than the fine fibrous fat unit 513a.
- the fat unit 513a in the form of fine fibers, the fat unit 513b in the form of fibers, the fat unit 513c in the form of thinly spread plates, or the fat unit in the form of lumps Artificial meat may be manufactured using the fat unit 513d and/or the fiber-shaped fat unit 513e having a non-circular cross section.
- these fat units 513 can be produced in various ways, such as extruding through a nozzle, extruding through a fine gap, processing a crosslinked second extruded raw material, or using a molding die. You will be able to.
- the step of culturing the cells may be performed when the cells are encapsulated in the first extrusion power source material or the second extrusion power source material, and since this may be performed in the same or similar manner as the above-described embodiments, a description thereof will be omitted. .
- the user forms artificial meat based on edible fibers and/or fat units.
- this step may be performed by manually processing the obtained edible fiber and/or fat unit, or may be performed by a printer that performs bio-3D printing.
- a description will be made based on the fact that a user produces artificial meat with marbling using edible fiber and fat units, but the present embodiment is not limited thereto.
- the user when a user intends to manufacture artificial meat composed of only lean meat parts, the user manufactures artificial meat using only edible fibers, and when manufacturing artificial meat composed of only fat parts, the user manufactures artificial meat using only fat units. can do.
- At least one edible fiber is folded or a plurality of edible fibers are arranged in a bundle to obtain an edible fiber structure (514; see FIG. 14) simulating the bundle structure of muscle fibers.
- a user may place at least one fat unit between these edible fiber structures to produce artificial meat with marbling.
- the marbling may have different patterns depending on how the combination of the edible fiber and the fat unit is formed.
- the user prepares artificial meat with marbling by combining a fat structure (515; see FIG. 15) in which at least one fat unit processed into a fiber form is folded or connected in a bundle form and the edible fiber structure 514 described above. Maybe.
- artificial meat may be formed as follows.
- the step of obtaining the edible fiber (S331) and the step of obtaining the fat unit (S332) may be performed by operating the 3D printer and discharging the edible fiber and the fat unit from the nozzle.
- the step of forming artificial meat may be performed by manufacturing an artificial meat model with edible fibers and/or fat units ejected from a 3D printer.
- edible fiber and/or fat units discharged from the printer may be adhered to each other in the process of printing artificial meat by applying an adhesive to the surface.
- the 3D printer may include a device for spraying adhesive, similar to the extrusion device 7 according to the second embodiment.
- the user may first model an artificial meat model.
- the artificial meat model may be a model having a certain pattern by a combination of the edible fiber structure 514 and the fat structure 515.
- the user may adjust the shape of the marbling appearing in the artificial meat by setting different combinations of the edible fiber structure 514 and the fat structure 515.
- the 3D printer may be set to print the artificial meat based on the input artificial meat model.
- the artificial meat manufacturing method according to the third embodiment may also process additive raw materials and place them in artificial meat.
- Artificial meat according to the above-described embodiments of the present invention has the advantage of obtaining cultured meat and artificial meat having characteristics similar to real meat by being applied to cultured meat, vegetable substitute meat, insect protein-based substitute meat, etc. there is.
- by selectively configuring the composition of the raw material it can be provided as a customized nutritious food to consumers of various constitutions.
- it will be possible to apply it as an oral administration method medicine by containing pharmaceutical ingredients in raw materials.
- the pattern due to the combination of the edible fiber (or the first extruded fiber) and the fat unit (or the second extruded fiber) is changed according to the part of the actual meat to be copied. This has the advantage of being able to simulate the marbling of a specific area.
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Abstract
Description
Claims (19)
- 살코기의 맛을 구현하는 원료가 가용화된 제1 압출전원료가 형성되는 단계;상기 제1 압출전원료가 압출되어, 압출 방향에 따른 길이를 갖는 적어도 하나의 제1 압출 섬유가 획득되는 단계;지방의 맛을 구현하는 원료가 가용화된 제2 압출전원료가 형성되는 단계;상기 제2 압출전원료가 압출되어, 압출 방향에 따른 길이를 갖는 적어도 하나의 제2 압출 섬유가 획득되는 단계; 및적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유가 상기 길이 방향을 따라 연결된 인공육이 획득되는 단계;를 포함하는 인공육 제조 방법.
- 제1 항에 있어서,상기 제1 압출 섬유가 획득되는 단계에서는, 적어도 하나의 제1 노즐을 통하여 상기 제1 압출전원료가 압출되고,상기 제2 압출 섬유가 획득되는 단계에서는, 적어도 하나의 제2 노즐을 통하여 상기 제2 압출전원료가 압출되는, 인공육 제조 방법.
- 제2 항에 있어서,상기 인공육이 획득되는 단계에서,적어도 하나의 상기 제1 노즐과 적어도 하나의 상기 제2 노즐의 배열에 의해, 상기 인공육은 상기 길이 방향에 교차하는 방향으로 절단된 단면에 상기 제1 압출 섬유와 상기 제2 압출 섬유로 형성된 패턴을 갖는, 인공육 제조 방법.
- 제3 항에 있어서,상기 인공육이 상기 길이 방향에 교차하는 방향으로 절단되어, 상기 패턴에 의한 마블링을 갖는 적어도 하나의 인공육 피스가 획득되는 단계;를 더 포함하는, 인공육 제조 방법.
- 제2 항에 있어서,상기 제1 압출 섬유가 획득되는 단계에서는, 상기 제1 노즐을 통과한 상기 제1 압출전원료에 가교제가 분사되어, 가교된 상기 제1 압출 섬유가 획득되고,상기 제2 압출 섬유가 획득되는 단계에서는, 상기 제2 노즐을 통과한 상기 제2 압출전원료에 가교제가 분사되어, 가교된 상기 제2 압출 섬유가 획득되는, 인공육 제조 방법.
- 제2 항에 있어서,상기 제1 압출 섬유가 획득되는 단계에서는, 상기 제1 노즐을 통과한 상기 제1 압출전원료가 가교제에 침지되어, 가교된 상기 제1 압출 섬유가 획득되고,상기 제2 압출 섬유가 획득되는 단계에서는, 상기 제2 노즐을 통과한 상기 제2 압출전원료가 가교제에 침지되어, 가교된 상기 제2 압출 섬유가 획득되는, 인공육 제조 방법.
- 제2 항에 있어서,상기 제1 압출 섬유가 획득되는 단계에서는, 상기 제1 노즐을 통과한 상기 제1 압출전원료가 겔화(gelation)를 위한 환경에 노출되어, 겔화된 상기 제1 압출 섬유가 획득되고,상기 제2 압출 섬유가 획득되는 단계에서는, 상기 제2 노즐을 통과한 상기 제2 압출전원료가 겔화를 위한 환경에 노출되어, 겔화된 상기 제2 압출 섬유가 획득되는, 인공육 제조 방법.
- 제1 항에 있어서,상기 인공육이 획득되는 단계에서는,상기 제1 압출 섬유 및 상기 제2 압출 섬유 중 적어도 하나에 접착제가 도포되어, 적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유가 상기 길이 방향을 따라 연결되는, 인공육 제조 방법.
- 제1 항에 있어서,상기 인공육이 획득되는 단계에서는,상기 제1 압출 섬유 및 상기 제2 압출 섬유가 접착제에 침지되어, 적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유가 상기 길이 방향을 따라 연결되는, 인공육 제조 방법.
- 제1 항에 있어서,상기 인공육이 획득되는 단계에서는,적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유를 상기 길이 방향을 따라 배열한 상태에서 세포를 배양하여, 배양된 세포들에 의해 적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유가 연결되는, 인공육 제조 방법.
- 제1 항에 있어서,영양소 및 의약성분 중 적어도 하나를 포함하는 첨가 원료가 가용화된 제3 압출전원료가 형성되는 단계; 및상기 제3 압출전원료가 압출되어, 압출 방향에 따른 길이를 갖는 적어도 하나의 제3 압출 섬유가 획득되는 단계;를 더 포함하고,상기 인공육이 획득되는 단계에서는, 적어도 하나의 상기 제1 압출 섬유, 적어도 하나의 상기 제2 압출 섬유 및 적어도 하나의 제3 압출 섬유가 상기 길이 방향을 따라 연결되는, 인공육 제조 방법.
- 살코기의 맛을 구현하는 원료가 섬유 형태로 가공된 식용 섬유가 획득되는 단계; 및적어도 하나의 상기 식용 섬유를 접거나 복수의 상기 식용 섬유를 다발 형태로 연결하여, 실제 육류에서 근섬유에 의한 다발 구조와 유사한 구조를 포함하는 인공육이 형성되는 단계;를 포함하는 인공육 제조 방법.
- 제12 항에 있어서,지방의 맛을 구현하는 원료가 겔화 또는 가교된 지방 유닛이 획득되는 단계;를 더 포함하고,상기 인공육이 형성되는 단계에서는, 적어도 하나의 상기 식용 섬유와 적어도 하나의 상기 지방 유닛을 조합하여, 상기 조합에 의한 패턴으로 실제 육류의 마블링이 구현된 인공육이 형성되는, 인공육 제조 방법.
- 제13 항에 있어서,상기 지방 유닛이 획득되는 단계에서, 상기 지방 유닛은 섬유 형태로 가공되어 획득되고,상기 인공육이 형성되는 단계에서 상기 인공육은,적어도 하나의 상기 식용 섬유를 접거나 복수의 상기 식용 섬유를 다발 형태로 연결한 식용 섬유 구조체와, 적어도 하나의 상기 지방 유닛을 접거나 복수의 상기 지방 유닛을 다발 형태로 연결한 지방 구조체의 조합으로 형성되는, 인공육 제조 방법.
- 제13 항에 있어서,상기 인공육이 형성되는 단계에서는,모사하고자 하는 상기 실제 육류의 부위에 따라, 상기 식용 섬유와 상기 지방 유닛의 조합으로 인한 패턴을 변경하여, 상기 부위에 따른 마블링을 구현하는, 인공육 제조 방법.
- 제12 항에 있어서,상기 인공육이 특정한 동물의 육류에 유사하도록, 상기 살코기의 맛을 구현하는 원료에 상기 특정한 동물의 세포가 봉입되는 단계;를 더 포함하는, 인공육 제조 방법.
- 인공육의 제1 원료를 가용화한 제1 압출전원료가 압출되어 형성되며, 압출 방향에 따른 길이를 갖는 적어도 하나의 제1 압출 섬유; 및상기 인공육의 제2 원료를 가용화한 제2 압출전원료가 압출되어 형성되며, 압출 방향에 따른 길이를 갖는 적어도 하나의 제2 압출 섬유;를 포함하고,적어도 하나의 상기 제1 압출 섬유와 적어도 하나의 상기 제2 압출 섬유가 상기 길이 방향을 따라 연결되어 형성되는, 인공육.
- 살코기의 맛을 구현하는 원료가 섬유 형태로 가공되어 형성되는 적어도 하나의 식용 섬유;를 포함하는 인공육에 있어서,상기 인공육이 실제 육류에서 근섬유에 의한 다발 구조와 유사한 구조를 포함하도록, 상기 인공육의 적어도 일부는 적어도 하나의 상기 식용 섬유를 접거나 복수의 상기 식용 섬유를 다발 형태로 연결하여 형성되는, 인공육.
- 제18 항에 있어서,지방의 맛을 구현하는 원료가 겔화 또는 가교된 지방 유닛;을 더 포함하고,상기 인공육에는, 적어도 하나의 상기 식용 섬유와 적어도 하나의 상기 지방 유닛의 조합에 의한 패턴으로 실제 육류의 마블링이 구현되는, 인공육.
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| CA3235364A CA3235364A1 (en) | 2021-10-12 | 2021-11-05 | An artificial meat and a method of making the artificial meat |
| EP21960734.8A EP4417064A4 (en) | 2021-10-12 | 2021-11-05 | Artificial meat and method for producing artificial meat |
| AU2021468103A AU2021468103B2 (en) | 2021-10-12 | 2021-11-05 | Artificial meat and method for manufacturing artificial meat |
| JP2024523111A JP7853410B2 (ja) | 2021-10-12 | 2021-11-05 | 人工肉および人工肉の製造方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025010507A1 (en) * | 2023-07-13 | 2025-01-16 | New School Foods Inc. | Process of producing a food analogue comprising of a plurality of phases |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102747536B1 (ko) * | 2023-12-01 | 2024-12-31 | 주식회사 티센바이오팜 | 대량 생산을 위한 확장형 인공육 생산 장치 |
| KR102739577B1 (ko) * | 2024-06-12 | 2024-12-05 | 경상국립대학교산학협력단 | 식용단백질 섬유 및 근육 형태 인공모조육의 제조방법 |
| KR102945390B1 (ko) * | 2025-03-24 | 2026-03-30 | 주식회사 티센바이오팜 | 압출 소재에 기반한 유닛 구조체의 성형 방법 |
| KR102945409B1 (ko) * | 2025-03-24 | 2026-03-30 | 주식회사 티센바이오팜 | 압출 소재에 기반한 유닛 구조체의 성형 방법 |
| KR102906975B1 (ko) * | 2025-03-24 | 2026-01-02 | 주식회사 티센바이오팜 | 압출 소재 생산을 위한 세포 혼합 모듈 제어 방법 |
| KR102906979B1 (ko) * | 2025-03-24 | 2026-01-06 | 주식회사 티센바이오팜 | 인공육 제조 방법 |
| KR102906976B1 (ko) * | 2025-03-24 | 2026-01-07 | 주식회사 티센바이오팜 | 압출 소재의 후처리 공정을 수행하는 장치 및 이의 제어 방법 |
| KR102906978B1 (ko) * | 2025-03-24 | 2026-01-06 | 주식회사 티센바이오팜 | 인공육 제조 장치의 관제 시스템 |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160066612A1 (en) * | 2014-09-04 | 2016-03-10 | Microjet Technology Co., Ltd. | Three-dimensional forming apparatus and method for manufacturing meat substitute |
| KR20200050165A (ko) * | 2018-11-01 | 2020-05-11 | 세종대학교산학협력단 | 올레오젤과 하이드로콜로이드가 혼합하여 제조된 식물성 지방 조성물 |
| WO2020152689A1 (en) * | 2019-01-26 | 2020-07-30 | Redefine Meat Ltd. | Meat analogues and methods of producing the same |
| KR20210094554A (ko) * | 2018-11-23 | 2021-07-29 | 발네바 에스이 | 조류 줄기 세포를 포함하는 식품 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1379600A (en) * | 1971-01-12 | 1975-01-02 | Pedigree Petfoods Ltd | Meat-like protein product |
| US4235935A (en) * | 1978-02-23 | 1980-11-25 | The Quaker Oats Company | Process for the production of a bacon analogue |
| US4539210A (en) * | 1978-08-07 | 1985-09-03 | Peter M. O'Connell | Process for making a structured meat product |
| US6835390B1 (en) * | 2000-11-17 | 2004-12-28 | Jon Vein | Method for producing tissue engineered meat for consumption |
| AU2002952721A0 (en) * | 2002-11-18 | 2002-12-05 | Mars, Incorporated | Meat analogue of authentic appearance |
| US20140220217A1 (en) * | 2011-07-12 | 2014-08-07 | Maraxi, Inc. | Method and compositions for consumables |
| KR101940063B1 (ko) * | 2016-11-30 | 2019-01-18 | 전영권 | 3d 인쇄 기술을 이용한 식품 성형방법 |
| MX2021001422A (es) * | 2018-08-07 | 2021-06-18 | Novameat Tech S L | Producto microextruido comestible que comprende proteinas. |
| WO2020208104A1 (en) * | 2019-04-10 | 2020-10-15 | Société des Produits Nestlé S.A. | Meat analogues and meat analogue extrusion devices and methods |
| JP2022527444A (ja) * | 2019-04-10 | 2022-06-02 | ソシエテ・デ・プロデュイ・ネスレ・エス・アー | 肉類似物及び肉類似物押出装置及び方法 |
| US20200332249A1 (en) * | 2019-04-18 | 2020-10-22 | Triton Algae Innovations, Inc. | Production of iron-complexed proteins from algae |
| KR20220123637A (ko) * | 2019-11-12 | 2022-09-08 | 리디파인 미트 엘티디. | 전 근육성 육류 대용품 및 이를 수득하는 방법 |
| IL273546B (en) * | 2020-03-24 | 2021-10-31 | Redefine Meat Ltd | Textured protein packaging and its uses |
| WO2023052130A1 (en) * | 2021-10-01 | 2023-04-06 | Eth Zurich | An extrusion system and method for obtaining a meat analogue |
-
2021
- 2021-10-12 KR KR1020210135323A patent/KR102518909B1/ko active Active
- 2021-11-05 CN CN202180104884.3A patent/CN118368992A/zh active Pending
- 2021-11-05 WO PCT/KR2021/015993 patent/WO2023063468A1/ko not_active Ceased
- 2021-11-05 AU AU2021468103A patent/AU2021468103B2/en active Active
- 2021-11-05 US US18/252,592 patent/US20240000128A1/en active Pending
- 2021-11-05 EP EP21960734.8A patent/EP4417064A4/en active Pending
- 2021-11-05 CA CA3235364A patent/CA3235364A1/en active Pending
-
2022
- 2022-12-20 KR KR1020220179407A patent/KR102811670B1/ko active Active
-
2025
- 2025-03-11 KR KR1020250031006A patent/KR20250040611A/ko active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160066612A1 (en) * | 2014-09-04 | 2016-03-10 | Microjet Technology Co., Ltd. | Three-dimensional forming apparatus and method for manufacturing meat substitute |
| KR20200050165A (ko) * | 2018-11-01 | 2020-05-11 | 세종대학교산학협력단 | 올레오젤과 하이드로콜로이드가 혼합하여 제조된 식물성 지방 조성물 |
| KR20210094554A (ko) * | 2018-11-23 | 2021-07-29 | 발네바 에스이 | 조류 줄기 세포를 포함하는 식품 |
| WO2020152689A1 (en) * | 2019-01-26 | 2020-07-30 | Redefine Meat Ltd. | Meat analogues and methods of producing the same |
Non-Patent Citations (2)
| Title |
|---|
| KO HYUN JUNG, WEN YAXIN, CHOI JI HO, PARK BO RAM, KIM HYUN WOO, PARK HYUN JIN: "Meat analog production through artificial muscle fiber insertion using coaxial nozzle-assisted three-dimensional food printing", FOOD HYDROCOLLOIDS, ELSEVIER BV, NL, vol. 120, 1 November 2021 (2021-11-01), NL , pages 106898, XP093058087, ISSN: 0268-005X, DOI: 10.1016/j.foodhyd.2021.106898 * |
| See also references of EP4417064A4 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025010507A1 (en) * | 2023-07-13 | 2025-01-16 | New School Foods Inc. | Process of producing a food analogue comprising of a plurality of phases |
| US12471608B2 (en) | 2023-07-13 | 2025-11-18 | Nstx Industries Inc. | Process of producing a food analogue precursor comprising of a plurality of phases |
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| Publication number | Publication date |
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| AU2021468103B2 (en) | 2025-09-18 |
| JP2024538163A (ja) | 2024-10-18 |
| CA3235364A1 (en) | 2023-04-20 |
| CN118368992A (zh) | 2024-07-19 |
| KR102518909B1 (ko) | 2023-04-06 |
| EP4417064A1 (en) | 2024-08-21 |
| KR20250040611A (ko) | 2025-03-24 |
| AU2021468103A1 (en) | 2024-05-02 |
| US20240000128A1 (en) | 2024-01-04 |
| KR20230052256A (ko) | 2023-04-19 |
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| EP4417064A4 (en) | 2025-03-26 |
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