WO2023164137A1

WO2023164137A1 - Multiple-tube pinch valve assembly

Info

Publication number: WO2023164137A1
Application number: PCT/US2023/013826
Authority: WO
Inventors: Andrew Gloor; Brian William Ward; Alexander Brown; Briden Ray Stanton; Brett BAEVERSTAD; Chuankun WANG; Stuart MCKNIGHT; Daniel Clement
Original assignee: Terumo BCT Inc
Current assignee: Terumo BCT Inc
Priority date: 2022-02-28
Filing date: 2023-02-24
Publication date: 2023-08-31
Anticipated expiration: 2024-08-28
Also published as: CN118765358A; EP4487033A4; US12152699B2; JP2025507708A; EP4487034A4; US20230272863A1; US20230272862A1; EP4487034A1; US12209689B2; EP4487033A1; JP7836899B2; WO2023164133A1; JP2025507659A; JP7814531B2; CN118541557A

Abstract

A multi-tubing pinch valve assembly including a receiving space defined between a pinch protrusion and tubing contact body. The multi-tubing pinch valve assembly is moveable between a retracted state and an extended state. In the extended state, multiple tubes disposed in the receiving space are pinched, restricting flow therethrough. In the retracted state, multiple tubes are capable of being loaded into and/or out of the receiving space.

Description

MULTIPLE-TUBE PINCH VALVE ASSEMBLY

CROSS-REFERENCE TO RELATED PATENT APPLICATION

[0001] This application claims priority to United States Patent Application No. 18/113,173, filed February 23, 2023, and the benefit of United States Provisional Patent Application No. 63/314,931 filed on February 28, 2022, the entire disclosures of which are incorporated herein by reference.

BACKGROUND

[0002] The present disclosure is generally directed to pinch valves, in particular, toward pinch valves for selectively controlling flow through multiple tubes.

[0003] In general, internal and/or external valves have been used to selectively control the flow of a fluid (e.g., a gas, a liquid, etc., and/or combinations thereof) through tubing or some other conduit. These valves may be operated to completely or partially block fluid flow. While internal valves are arranged at least partially within a lumen of the tubing, external valves allow fluid flow control from outside of the tubing. In particular, an external valve allows a force to be applied to an external surface of the tubing, or soft conduit, deforming the lumen of the tubing from an unrestricted state to an occluded, or partially occluded, state.

BRIEF SUMMARY

[0004] External valves (e.g., pinch valves, etc.) provide the ability to control fluid flow in tubing without requiring the installation of expensive and complex internal valves. Typical pinch valves control fluid flow in tubing by compressing an outer portion of the tubing thereby closing a lumen disposed in an inner portion of the tubing. As can be appreciated, pinch valves offer a sterile fluid flow control for tubing where the components of the pinch valve do not come into direct contact with fluid inside the tubing.

[0005] In some examples, a tubing arrangement or system design may require that more than one tube, or section of tubing, be closed at the same time or at different times. In conventional systems, a pinch valve may be positioned at each discrete area where fluid flow control may be desired. Each pinch valve may be independently operated by a respective actuator. In this conventional approach, as the number of the discrete fluid flow control areas increases, so does the number of pinch valves required to service the system. Since each pinch valve includes its own actuator, the cost and complexity of this conventional approach necessarily increases. In addition, the number of components required to perform the fluid control operations under the conventional approach increases the potential for failure and the time required for routine maintenance operations.

[0006] It is with respect to the above issues and other problems that the embodiments presented herein were contemplated. Among other things, the present disclosure provides a multiple-tubing, or multi-tubing, pinch valve assembly that is capable of pinching more than one tube, or section of tubing, with a single actuator. In some examples, the multi-tubing pinch valve assembly may be configured to pinch various tubes, or tubing sections, simultaneously. Additionally or alternatively, the multi-tubing pinch valve assembly may pinch a first set of tubes at a first time and pinch a second set of tubes at a different second time. In one example, the multi-tubing pinch valve assembly may allow flow through the first set of tubes while pinching the second set of tubes (e.g., preventing flow, etc.), and vice versa.

[0007] In one example, the multi-tubing pinch valve assembly may comprise a compact valve array that is capable of executing a sequence of opening and closing flow paths to each bioreactor in a multiple-bioreactor system. The multi-tubing pinch valve assembly may comprise a valve mechanism that may be built into a bioreactor rocker assembly. This design, among other things, greatly simplifies a disposable set (e.g., tubing set, etc.) used in cell expansion systems, sterile fluid management systems, any suitable system that uses a multi-tubing valving scheme for sterile fluid pathway management, etc. Additionally or alternatively, the multi-tubing pinch valve assembly may integrate with a soft cassette of a disposable set.

[0008] In some examples, the multi-tubing pinch valve assembly may correspond to a cam- driven valve array that provides a compact method of opening/closing many parallel tubes in a set sequence. This approach may allow a multiple-bioreactor cell expansion system to execute a “time share” or “duty cycle” method of servicing multiple bioreactors, with the valve array being located on the bioreactor rocker rather than on a static cassette. As can be appreciated, the multi-tubing pinch valve assembly is not limited to use in a multiple-bioreactor system. For instance, the mechanism of the multi-tubing pinch valve assembly may be adapted to any situation where multiple valves may be required to have their positions fixed relative to others.

[0009] In a cam-driven approach, the multi-tubing pinch valve assembly may utilize a finger pump type mechanism having the orientation of the tube and fingers rotated 90 degrees relative to the cam. Rather than pumping a single tube, the multi-tubing pinch valve assembly is capable of valving many tubes simultaneously. This multi-tubing pinch valve assembly design can be paired with a soft cassette, as the fingers actuate in a direction normal to the soft cassette plane. The multi-tubing pinch valve assembly allows the occlusion depth to be tightly controlled, which allows for the possibility of intentionally partially occluding tubes (e.g., partial restriction of fluid flow, etc.). Another benefit of the examples described herein include the compact size and simplified electronics of the multi-tubing pinch valve assembly. Among other things, these features allow the valve unit to be mounted to a bioreactor rocker, in some cases, thereby simplifying a disposable set used in cell expansion systems, for example, by reducing the number of tubes that must run from the bioreactors to the static cassette. Further, the multi-tubing pinch valve assembly may ensure that valve positions are locked relative to each other, allowing assurance of correct flow. For example, it can be assured that one of the tubes in the time share model is always open, preventing pressure buildup, or two tubes may always be open or closed relative to each other, assuring a complete fluid path.

[0010] In some examples, the multi-tubing pinch valve assembly may be driven by a motor operatively connected to a camshaft comprising two cam profiles. A first anvil assembly may be disposed adjacent a first cam profile of the two cam profiles and a second anvil assembly may be disposed adjacent a second cam profile of the two cam profiles. As the motor rotates the camshaft, the two cam profiles rotate relative to the first anvil assembly and the second anvil assembly. At specific angles of rotation, at least one cam lobe, or protrusion, disposed on the cam profile may contact the first anvil assembly and the second anvil assembly. For instance, a first cam lobe associated with the first cam profile may contact the first anvil assembly at a first angle of rotation of the camshaft. As the first cam lobe contacts the first anvil assembly, the shape of the first cam lobe may cause the first anvil assembly to move in a direction away from the camshaft toward a pinch plate. A first section of tubing may be disposed in a first space between the first anvil and the pinch plate. In this arrangement, as the first anvil assembly moves toward the pinch plate, a first anvil of the first anvil assembly may contact and deform the first section of tubing such that a lumen of the first section of tubing closes. The first section of tubing may remain closed while the first cam lobe is at, or within a predetermined angular range (e.g., 1-5 degrees) of, a highest displacement point of the first cam lobe and the first anvil maintains the first section of tubing in a closed state.

[0011] The multi-tubing pinch valve assembly may be configured as an array of independently actuated multiple-tubing pinch valves. For instance, the multi-tubing pinch valve assembly may include a five-valve array of independently actuated four-tube pinch valves. At least one benefit to this independent actuation arrangement includes, but is in no way limited to, operating the multi-tubing pinch valve assembly in any combination of open or closed states.

[0012] This present disclosure describes, among other things, a multi-tubing pinch valve assembly comprising an array that is split into discrete multi-tube valves (e.g., an array of five 4- tube pinch valves), each multi-tube valve in the array capable of being driven by a small linear actuator (e.g., a solenoid, piezoelectric actuator, screw-type actuator, pneumatic cylinder, hydraulic cylinder, and/or a stepper motor linear actuator, etc.). This approach allows for any combination of valve states during a protocol (e.g., system operation, etc.). For example, in a cell expansion system, all valves may be opened during loading of a disposable (e.g., tubing set, etc.), valves may cycle continuously on a 10-second duty cycle during a cell feed, and valves may be opened sequentially for longer durations during tasks such as cell harvest, and/or the like.

[0013] In some examples, the pinching jaws of each multi-tube valve (e.g., 4-tube valve, etc.) of the multi-tubing pinch valve assembly may be slightly offset from one another. Among other things, this arrangement may reduce the pinch force from the actuator that may be required to occlude the tubing and increase the jaw gap range that results in occlusion. Such an increased jaw gap may provide enhanced cycling life and tubing integrity over time compared to designs including a decreased jaw gap. In one example, a single set of opposing jaws may pinch four tubes at once. Additionally or alternatively, the independent operability of each of the multi-tube valves may allow for disposable design optimization and simplification when compared with designs that require different valving.

[0014] The preceding is a simplified summary of the disclosure to provide an understanding of some aspects of the disclosure. This summary is neither an extensive nor exhaustive overview of the disclosure and its various aspects, embodiments, and configurations. It is intended neither to identify key or critical elements of the disclosure nor to delineate the scope of the disclosure but to present selected concepts of the disclosure in a simplified form as an introduction to the more detailed description presented below. As will be appreciated, other aspects, embodiments, and configurations of the disclosure are possible utilizing, alone or in combination, one or more of the features set forth above or described in detail below.

[0015] Numerous additional features and advantages are described herein and will be apparent to those skilled in the art upon consideration of the following Detailed Description and in view of the figures.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

[0016] The accompanying drawings are incorporated into and form a part of the specification to illustrate several examples of the present disclosure. These drawings, together with the description, explain the principles of the disclosure. The drawings simply illustrate preferred and alternative examples of how the disclosure can be made and used and are not to be construed as limiting the disclosure to only the illustrated and described examples. Further features and advantages will become apparent from the following, more detailed, description of the various aspects, embodiments, and configurations of the disclosure, as illustrated by the drawings referenced below.

[0017] Fig. 1A shows a first perspective view of a multi-tubing pinch valve assembly in accordance with embodiments of the present disclosure;

[0018] Fig. IB shows a front elevation view of the multi-tubing pinch valve assembly shown in Fig. 1A;

[0019] Fig. 1C shows a second perspective view of the multi-tubing pinch valve shown in Fig. 1A;

[0020] Fig. ID shows a partially exploded perspective view of the multi-tubing pinch valve assembly shown in Fig. 1A;

[0021] Fig. 2A shows a schematic section view of the multi-tubing pinch valve assembly taken through line 2-2 shown in Fig. ID;

[0022] Fig. 2B shows a perspective view of an anvil assembly in accordance with examples of the present disclosure;

[0023] Fig. 3A is a schematic section view of the anvil assembly and cam of a multi-tubing pinch valve assembly at a first rotational position in accordance with examples of the present disclosure;

[0024] Fig. 3B is a schematic section view of the anvil assembly and cam of a multi-tubing pinch valve assembly at a second rotational position in accordance with examples of the present disclosure;

[0025] Fig. 3C is a schematic section view of the anvil assembly and cam of a multi-tubing pinch valve assembly at a third rotational position in accordance with examples of the present disclosure;

[0026] Fig. 4A shows a first perspective view of a multi-tubing pinch valve assembly in accordance with embodiments of the present disclosure;

[0027] Fig. 4B shows a plan view of the multi-tubing pinch valve assembly shown in Fig. 4A;

[0028] Fig. 5A shows a schematic plan view of the multi-tubing pinch valve assembly shown in Fig. 4A in a closed, tubing-occluded, state in accordance with examples of the present disclosure;

[0029] Fig. 5B shows a schematic plan view of the multi-tubing pinch valve assembly shown in Fig. 4A in a partially closed, tubing-partially-occluded, state in accordance with examples of the present disclosure;

[0030] Fig. 5C shows a schematic plan view of the multi-tubing pinch valve assembly shown in Fig. 4A in an open, tubing-open, state in accordance with examples of the present disclosure; [0031] Fig. 6A shows a first perspective view of a multi-tubing pinch valve assembly in an array configuration in accordance with examples of the present disclosure;

[0032] Fig. 6B shows a second perspective view of a multi-tubing pinch valve assembly in an array configuration in accordance with examples of the present disclosure;

[0033] Fig. 7 shows a perspective view of a multi-tubing pinch valve assembly in an array configuration for a multiple-bioreactor system;

[0034] Fig. 8 is a schematic block diagram of a hydraulic layout for a cell expansion system having a single bioreactor in accordance with examples of the present disclosure;

[0035] Fig. 9 is a schematic block diagram of a hydraulic layout for a cell expansion system having multiple bioreactors and a multi-tubing pinch valve assembly in accordance with examples of the present disclosure;

[0036] Fig. 10 is a perspective view of another exemplary multitube pinch valve assembly in accordance with the present disclosure;

[0037] Fig. 11 is a perspective view of the multitube pinch valve assembly of Fig. 10 with a cover removed;

[0038] Fig. 12 is a perspective view of an exemplary actuation member of the multitube pinch valve assembly of Fig. 10;

[0039] Fig. 13 is a perspective view of a bioreactor support frame configured for the multitube pinch valve assembly of Fig. 10;

[0040] Fig. 14 is a perspective view of an additional exemplary multitube pinch valve assembly in accordance with the present disclosure;

[0041] Fig. 15 is a perspective view of the multitube pinch valve assembly of Fig. 14 with a bioreactor support frame removed;

[0042] Fig. 16 is a perspective view of the multitube pinch valve assembly of Fig. 14 with a cover removed;

[0043] Fig. 17 is a cross-sectional view of the multitube pinch valve assembly of Fig. 14; and [0044] Fig. 18 is a perspective view of a bioreactor support frame configured for the multitube pinch valve assembly of Fig. 14.

DETAILED DESCRIPTION

[0045] Before any embodiments of the disclosure are explained in detail, it is to be understood that the disclosure is not limited in its application to the details of construction and the arrangement of components set forth in the following description or illustrated in the drawings. The disclosure is capable of other embodiments and of being practiced or of being carried out in various ways. Also, it is to be understood that the phraseology and terminology used herein is for the purpose of description and should not be regarded as limiting. The use of “including,” “comprising,” or “having” and variations thereof herein is meant to encompass the items listed thereafter and equivalents thereof as well as additional items. Further, the present disclosure may use examples to illustrate one or more aspects thereof. Unless explicitly stated otherwise, the use or listing of one or more examples (which may be denoted by “for example,” “by way of example,” “e.g.,” “such as,” or similar language) is not intended to and does not limit the scope of the present disclosure.

[0046] The ensuing description provides embodiments only, and is not intended to limit the scope, applicability, or configuration of the claims. Rather, the ensuing description will provide those skilled in the art with an enabling description for implementing the described embodiments. It being understood that various changes may be made in the function and arrangement of elements without departing from the spirit and scope of the appended claims.

[0047] Various aspects of the present disclosure will be described herein with reference to drawings that may be schematic illustrations of idealized configurations.

[0048] Conventional external valving solutions generally utilize a single pinch valve to control fluid flow in a single tube. When fluid flow control is required for multiple tubes, each tube of the multiple tubes may employ a respective pinch valve. As can be appreciated, this conventional arrangement increases the number of components needed for a multiple-tube fluid flow control application. With the increased number of components, the complexity of the conventional multiple-tube fluid flow control application increases. Additionally or alternatively, control of each pinch valve in a multiple-tube fluid flow control application, where each tube has its own pinch valve, requires complex valve controls, timing, wiring, and programming. In any event, employing a single pinch valve for every tube, or section of tubing, that is to be pinched in a conventional approach results in increased maintenance times, failures, and system complexity.

[0049] It is with respect to the above issues and other problems that the embodiments presented herein were contemplated.

[0050] Referring now to Figs. 1A-1D, a multiple-tube pinch valve assembly 100 is shown in accordance with embodiments of the present disclosure. The multiple-tube pinch valve assembly 100 may comprise a base 104 (e.g., baseplate, frame, support structure, etc.), a camshaft 108, at least one cam 112 attached to the camshaft 108, at least one anvil assembly 150, and a tubing platen 120. In some examples, the tubing platen 120 may comprise a plurality of tube receiving apertures 128 disposed along a length of the tubing platen 120. Each tube receiving aperture 128 of the plurality of tube receiving apertures 128 may be configured to receive a tube, or section of tubing, therein. The plurality of tube receiving apertures 128 may extend in a direction that is perpendicular to the longitudinal axis 110 of the camshaft 108.

[0051] Features of the multiple-tube pinch valve assembly 100 may be described in conjunction with a coordinate system 102. The coordinate system 102, as shown in the figures, includes three- dimensions comprising an X-axis, a Y-axis, and a Z-axis. Additionally or alternatively, the coordinate system 102 may be used to define planes (e.g., the XY-plane, the XZ-plane, and the YZ-plane) of the multiple-tube pinch valve assembly 100. These planes may be disposed orthogonal, or at 90 degrees, to one another. While the origin of the coordinate system 102 may be placed at any point on or near the components of the multiple-tube pinch valve assembly 100, for the purposes of description, the axes of the coordinate system 102 are always disposed along the same directions from figure to figure. In some examples, reference may be made to dimensions, angles, directions, relative positions, and/or movements associated with one or more components of the multiple-tube pinch valve assembly 100 with respect to the coordinate system 102. For example, the width of the multiple-tube pinch valve assembly 100 may be defined as a dimension along the X-axis of the coordinate system 102, the height of the multiple-tube pinch valve assembly 100 may be defined as dimension along the Y-axis of the coordinate system 102, and the depth of the multiple-tube pinch valve assembly 100 may be defined as a dimension along the Z-axis of the coordinate system 102. Additionally or alternatively, the width of the tubing platen 120 may be defined as a dimension along the X-axis of the coordinate system 102, the height of the tubing platen 120 may be defined as dimension along the Y-axis of the coordinate system 102, and the depth of the tubing platen 120 may be defined as a dimension along the Z- axis of the coordinate system 102.

[0052] The multiple-tube pinch valve assembly 100 may include a motor 132 having an output shaft 134, a drive body 136, and a cam drive body 140. As the output shaft 134 of the motor 132 rotates, power is transmitted from the drive body 136 to the cam drive body 140. In one example, the drive body 136 and the cam drive body 140 may be configured as respective gears in meshing contact with one another. In one example, the drive body 136 and the cam drive body 140 may correspond to pulleys (e.g., timing belt pulleys, V-pulleys, etc.). In this example, power may be transmitted from the drive body 136 to the cam drive body 140 via a drive belt 138. The drive belt 138 may correspond to a timing belt, a V-belt, a ribbed V-belt, a link V-belt, etc., and/or any other continuous belt. In any of these examples, as the output shaft 134 of the motor 132 rotates, the camshaft 108 is caused to rotate about the longitudinal axis 110. Rotation of the camshaft 108 causes the cam 112 having at least one cam profile shape to rotate about the longitudinal axis 110. The cam 112 may be keyed (e.g., via a key and keyway, a spline, etc.), fixed, formed from, and/or otherwise affixed to the camshaft 108. The cam 112 may rotate through 360 degrees. At various angular rotations, different portions of the cam profile shape of the cam 112 may be presented to an anvil assembly 150. For instance, as a protrusion of the cam 112 contacts the anvil assembly 150, the anvil assembly 150 may lift (e.g., move in the positive Y-axis direction) toward the tubing platen 120 and as a recess, or heel, of the cam 112 contacts the anvil assembly 150, the anvil assembly 150 may lower (e.g., move in the negative Y-axis direction) away from the tubing platen 120. In this manner, the rotation of the camshaft 108 may cause the anvil assembly 150 to pinch the multiple tubes 124 in the tube receiving apertures 128 of the multiple-tube pinch valve assembly 100.

[0053] In some examples, the multiple-tube pinch valve assembly 100 may include a bioreactor support frame 144. The bioreactor support frame 144 may comprise a plurality of recesses 142 configured to receive and/or hold a respective bioreactor (e.g., hollow fiber bioreactor, cell expansion system bioreactor, etc.). In some examples, the multiple tubes 124 that are disposed in the tube receiving apertures 128 may be interconnected to the multiple bioreactors that are held, or supported, by the bioreactor support frame 144. Fluid flow control to multiple bioreactors may benefit from altering the timing associated with opening and closing the multiple tubes 124 associated with each bioreactor at a same time or at different times.

[0054] As shown in the front elevation view of Fig. IB, the multiple-tube pinch valve assembly 100 may be configured to have a valve array 148A-148E, or an array of valves, comprising a first multi-tube valve 148A, a second multi-tube valve 148B, a third multi-tube valve 148C, a fourth multi-tube valve 148D, and/or a fifth multi-tube valve 148E. Each multi-tube valve of the valve array 148A-148E, may be associated with a set of tubes dedicated to a particular bioreactor. For instance, the first multi-tube valve 148A may control the fluid flow of multiple tubes 124 that are received in the tube receiving apertures 128 associated with the first multi-tube valve 148A. Continuing this example, the second multi-tube valve 148B may control the fluid flow of multiple tubes 124 that are received in the tube receiving apertures 128 associated with the second multitube valve 148B, and so on. Each multi-tube valve of the valve array 148A-148E may include a respective anvil assembly 150 and a respective cam 112. In this manner, the cam 112 may be arranged on the camshaft 108 such that one multi-tube valve of the valve array 148A-148E can be operated at an individual, or independent, timing relative to the other multi-tube valves in the valve array 148A-148E. Stated another way, a cam 112 of one multi-tube valve of the valve array 148A-148E may be rotationally oriented out of phase from at least one other multi-tube valve of the valve array 148A-148E. In this example, as the camshaft 108 rotates, the valves in the valve array 148A-148E may open and close at the same or different times. In some examples, the valve array 148A-148E may be disposed along a length of the longitudinal axis 110 (e.g., in the X-axis direction) of the camshaft 108. Although shown comprising five separate valves 148A-148E, the multi-tubing pinch valve assembly 100 may include any number of valves. In some examples, the number of valves may be configured to match a number of bioreactors or sets of multiple tubes 124 that require fluid flow control. As can be appreciated, the valve array of the multi-tubing pinch valve assembly 100 may comprise one, two, three, four, five, or more valves.

[0055] Fig. ID shows a partially exploded perspective view of the multi-tubing pinch valve assembly 100, exposing the anvil grating plate 116. The anvil grating plate 116 may comprise a number of apertures passing therethrough. The apertures may be arranged along the X-axis of the anvil grating plate 116. Each aperture may be sized to receive a portion of the anvil assembly 150. As the camshaft 108 rotates, the cam 112 may contact the anvil assembly 150 moving a portion of the anvil assembly 150 into and/or out of the apertures. The anvil grating plate 116 may comprise a top surface that is arranged a distance from a tubing platen contact surface of the tubing platen 120. This distance may provide a space in which the multiple tubes 124 are disposed. As can be appreciated, as the portion of the anvil assembly 150 enters this space, the multiple tubes 124 may be closed (e.g., pinched) and as the portion of the anvil assembly 150 leaves this space, the multiple tubes 124 may be opened. Additional detail regarding this cam-actuated pinching and fluid flow control of multiple tubes is described in conjunction with Figs. 2A-3C.

[0056] Fig. 2A shows a schematic section view of the multi-tubing pinch valve assembly taken through line 2-2 shown in Fig. ID. The camshaft 108 is shown having the cam 112 oriented rotationally in a position where the anvil assembly 150 is pinching the multiple tubes 124 disposed in the tube receiving apertures 128 (e.g., in the space between the anvil grating plate 116 and the tubing platen 120). In this position, the anvil assembly 150 is in contact with a cam lobe of the cam 112 and the multiple tubes 124 are shown in a pinched state. Fluid flow through the multiple tubes 124 is restricted in the pinched state where the anvil assembly 150 contacts the multiple tubes 124.

[0057] The anvil assembly 150, shown in greater detail in Fig. 2B, includes an anvil 200 comprising an anvil body 204 and a pinch edge 208 extending from a first end 212 A to a second end 212B of the anvil assembly 150. The anvil 200 may comprise a protrusion extending from at least one stop ledge 216 of the anvil body 204 in the Y-axis direction. The protrusion may be sized to engage with an aperture, or receptacle, in the anvil grating plate 116 (e.g., in a slip fit, clearance fit, and/or the like). The protrusion may terminate at the pinch edge 208. In some examples, the pinch edge 208 may be flat, radiused, or sharp. The pinch edge 208 may comprise a tapered portion that slopes away from the pinch edge 208 toward the stop ledge 216. In some examples, the tapered portion may allow a section of the multiple tubes 124 being pinched to flex and move around the pinch edge 208. This shape, among other things, may reduce stresses placed on the multiple tubes 124 during pinching.

[0058] The anvil assembly 150 may comprise at least one cam contact portion disposed on a lower portion of the anvil body 204. The at least one cam contact portion may extend past the anvil body 204. In any event, the at least one cam contact portion may provide a contact surface between the anvil body 204 and the cam 112 of the multiple-tube pinch valve assembly 100. In some examples, the at least one cam contact portion may correspond to a cam follower 210, or other bearing. The cam follower 210, in some cases, may be made to include a hardened steel wheel and a bearing (e.g., roller bearing, ball bearing, etc.). The lower portion of the anvil body 204 may include a tapered surface 220 disposed on either side of a plane running through a center of the anvil body 204 (e.g., along the XY-plane). The tapered surface 220 may provide clearance for portions of the cam 112 and the cam profile shape as the cam 112 moves relative to the anvil body 204 and anvil assembly 150.

[0059] Figs. 3A-3C shown schematic section views of the anvil assembly 150 as the cam 112 rotates about the longitudinal axis 110. A rotation position fiducial 302 is shown on the cam 112 to illustrate the position from a first angular reference (e.g., illustrated as a zero degree line). The cam 112 may comprise a cam profile shape (e.g., disposed around a periphery of the cam 112) including a cam heel 308 (e.g., a low point), a cam lobe 316 (e.g., a high point), and a cam transition 312 disposed between the cam heel 308 and the cam lobe 316. The cam heel 308 may correspond to a first point along the cam profile shape where a distance from the periphery of the cam 112 to the center of the cam 112 (e.g., the longitudinal axis 110 of the camshaft 108) is closest than other points along the cam profile shape. Additionally or alternatively, the cam lobe 316 may correspond to a second point along the cam profile shape where a distance from the periphery of the cam 112 to the center of the cam 112 (e.g., the longitudinal axis 110 of the camshaft 108) is further than other points along the cam profile shape. As can be appreciated, the distance from the periphery of the cam 112 to the center of the cam 112 at the cam transition 312 may be a distance that is between the distance measured at the first point and the second point.

[0060] In Figs. 3A-3C, the multiple tubes 124 are disposed in a space between the anvil grating plate 116 and the tubing platen 120. In some examples, this space may correspond to the tube receiving apertures 128 described above. Fig. 3A corresponds to the anvil assembly 150 at a first cam 112 rotational position, Fig. 3B corresponds to the anvil assembly 150 at a second cam 112 rotational position, and Fig. 3C corresponds to the anvil assembly 150 at a third cam 112 rotational position. In the first rotational position of the cam 112, the multiple tubes 124 are shown in an open fluid flow state. In this position, fluid may be allowed to flow through each of the multiple tubes 124. In the second rotational position of the cam 112, the multiple tubes 124 are shown in a partially occluded, restricted fluid flow, state. In this position, fluid may be allowed to flow through each of the multiple tubes 124 but in a restricted, partially closed, state. For example, the flow rate of fluid flowing through the multiple tubes 124 may be reduced in the second rotational position when compared to the first rotational position. In the third rotational position of the cam 112, the multiple tubes 124 are shown in a closed state. In this position, fluid is restricted from flowing through each of the multiple tubes 124. This third rotational position may correspond to a completely occluded tubing state, which may also be referred to herein as a pinched or pinched closed state of the multiple tubes 124.

[0061] As shown in Fig. 3A, the anvil assembly 150 is in a lowermost position, where the protrusion of the anvil 200 is disposed in the anvil aperture 304 of the anvil grating plate 116. In particular, the cam follower 210 of the anvil assembly 150 is disposed in contact with the cam heel 308 of the cam 112. In this lowermost position, the pinch edge 208 may be disposed adjacent the multiple tubes 124. The multiple tubes 124 are not pinched by the anvil 200 in Fig. 3A, allowing fluid flow through the multiple tubes 124 from the left-hand side of the page to the righthand side of the page, or vice versa.

[0062] When the camshaft 108 is rotated by a first rotation 320A (e.g., shown as a first clockwise rotation), the cam 112 rotates from the zero-degree reference to the al angular position (e.g., comprising a non-zero angle measured from the zero-degree reference), as shown in Fig. 3B. In the al angular position, the cam follower 210 of the anvil assembly 150 is shown in contact with the cam transition 312 of the cam 112. Since the distance from the periphery of the cam 112 at the cam transition 312 is greater than the distance from the periphery of the cam 112 at the cam heel 308, the anvil assembly 150 raises, or lifts, upward disposing the pinch edge 208 in the space between the anvil grating plate 116 and the tubing platen 120. More specifically, the pinch edge 208 contacts the multiple tubes 124 disposed in the tube receiving apertures 128 and deforms the multiple tubes 124 restricting flow therethrough. In the rotational position shown in Fig. 3B, the anvil assembly 150 deforms the multiple tubes 124 providing a partially occluded flow path 324 at least along the pinch edge 208 (e.g., extending into the page).

[0063] Continuing the rotation from Fig. 3B, the camshaft 108 is rotated by a second rotation 320B (e.g., shown as a second continuing clockwise rotation) further rotating the cam 112 from the al angular position to the a2 angular position. In the a2 angular position, the cam follower 210 of the anvil assembly 150 is shown in contact with the cam lobe 316 of the cam 112. Since the distance from the periphery of the cam 112 at the cam lobe 316 is greater than the distance from the periphery of the cam 112 measured at the cam transition 312, the anvil assembly 150 further raises, or lifts, upward disposing the pinch edge 208 further in the space between the anvil grating plate 116 and the tubing platen 120. In this raised position, the pinch edge 208 deforms the multiple tubes 124 disposed in the tube receiving apertures 128 completely restricting flow therethrough. In the rotational position shown in Fig. 3C, the anvil assembly 150 deforms the multiple tubes 124 providing a pinched tubing point 328 at least along the pinch edge 208 (e.g., extending into the page).

[0064] The cam 112 may also be configured to provide an “all-open” state in which all of the tubes 124 are open and not constricted by the anvil assembly 150. Such an “all open” state improves loading of the bioreactor support frame 144. The cam 112 may also be configured to be removable from the assembly 100 to allow all of the bioreactors to be opened simultaneously.

[0065] Although described as rotating in a clockwise direction, it should be appreciated that the camshaft 108 may be rotated in a counterclockwise direction, a clockwise direction, and/or combinations thereof. In some examples, the motor 132 may be rotated in any rotational direction causing the rotation of the camshaft 108. Additionally or alternatively, while described as having a cam heel 308, cam transitions 312, and a cam lobe 316, it should be appreciated that the cam 112 may include greater or fewer protrusions, shapes, dwells, lobes, and recesses than are shown and described in Figs. 1A-3C.

[0066] Referring to Figs. 4A and 4B, various views of a multi-tubing pinch valve assembly 400 is shown in accordance with embodiments of the present disclosure. The multi-tubing pinch valve assembly 400 may provide an array of pinch valves that utilize independently controllable hinged clamp mechanisms. In some examples, the multi-tubing pinch valve assembly 400 may include at least one base 404, hinge plate 416, and clamp platen 420. Multiple tubes 124 may be disposed between the hinge plate 416 and the clamp platen 420. In one example, the multiple tubes 124 may be disposed in tube receiving apertures 128 associated with each valve. The hinge plate 416 may be pivotally connected to a hinge support block 424 at a first point via a pivot axis. An actuator, for example, linear actuator 410, may be attached to a second point of the hinge plate 416. The linear actuator 410 may correspond to a solenoid, a screw-type actuator, and/or a stepper motor-actuated screw-type actuator.

[0067] The linear actuator 410 may be mounted to a mount body 408 that is fixed relative to the base 404 of the multi-tubing pinch valve assembly 400. As the linear actuator 410 is actuated, a translation rod 412 may move between a retracted state and an extended state, or vice versa. Extending the translation rod 412 may move the hinge plate 416 toward the clamp platen 420. In this extended position, the multiple tubes 124 disposed in the tube receiving aperture 128 may be pinched by one or more features of the hinge plate 416 and the clamp platen 420.

[0068] The clamp platen 420 may be fixed relative to the base 404. As the linear actuator 410 is actuated, the translation rod 412 may move the hinge plate 416 from an open state to a closed state, or vice versa. In the open state, at least one portion of the hinge plate 416 may be separated further from the clamp platen 420 than when in the closed state. When in the open state, the multiple tubes 124 may be loaded and/or removed from the tube receiving aperture 128. Stated another way, the multiple tubes 124 may not be clamped, or pinched, when in the open state. However, when in the closed state, the multiple tubes 124 may be pinched between the hinge plate 416 and the clamp platen 420. Additional detail regarding the construction of the hinge plate 416 and the clamp platen 420 and the fluid flow control (e.g., pinching) arrangement is described in conjunction with Figs. 5A-5C.

[0069] In some examples, the multi-tubing pinch valve assembly 400 may comprise an array of valves arranged adjacent to one another. As shown in the plan view of Fig. 4B, four separate valves are shown, each valve configured to pinch multiple tubes 124 associated therewith. Each valve of the multi-tubing pinch valve assembly 400 may comprise a linear actuator 410, a translation rod 412, and a hinge plate 416. Although shown with separate clamp platens 420 in Figs. 4A and 4B, it should be appreciated that different valves may share a clamp platen 420. For instance, and as shown in Figs. 6A and 6B, the dual clamp platen 620 is used by more than one valve. The array of valves of the multi-tubing pinch valve assembly 400 shown in Fig. 4B, show a first row comprising two valves with linear actuators 410 that are configured to extend from right to left and a second row comprising two valves with linear actuators 410 that are configured to extend from left to right. Although shown comprising four separate valves, the multi-tubing pinch valve assembly 400 may include any number of valves. In some examples, the number of valves may be configured to match a number of bioreactors or sets of multiple tubes 124 that require fluid flow control. As can be appreciated, the valve array of the multi-tubing pinch valve assembly 400 may comprise one, two, three, four, five, or more valves.

[0070] Figs. 5A-5C show schematic plan views of the multi-tubing pinch valve assembly 400 described above moving between a closed state and an open state. The linear actuator 410 is shown attached to a mount body 408 that is fixed to the base 404. A hinge plate 416 is shown pivotally attached to a hinge support block 424 that is fixed to the base 404. The clamp platen 420 may be fixed to the base 404 or other portion of the multi-tubing pinch valve assembly 400. The hinge plate 416 may comprise a hinge pinch protrusion 516 extending from a surface of the hinge plate 416 in a direction toward the clamp platen 420. In some examples, the clamp platen 420 may comprise a tubing contact portion that is configured to contact the multiple tubes 124 disposed in the tube receiving aperture 128. In some examples, the tubing contact portion may correspond to a flat plate surface and/or a platen pinch protrusion 520 that extends from a surface of the clamp platen 420 (e.g., in a direction of the hinge plate 416).

[0071] The linear actuator 410 may be attached to a mount body 408. The mount body 408 may be fixed relative to the base 404. The linear actuator 410 may include a translation rod 412 that moves relative to the mount body 408 (e.g., in a direction toward or away from the mount body 408). The translation rod 412 may comprise a clevis pin 504 that engages with a clevis of the hinge plate 416. In some examples, the hinge plate 416 may comprise a clevis slot 508 in which the clevis pin 504 may be disposed.

[0072] Fig. 5A shows a schematic plan view of the multi-tubing pinch valve assembly 400 in a closed, tubing-occluded, state in accordance with examples of the present disclosure. In this state, the translation rod 412 of the linear actuator 410 is extended by a first translation vector 502A. The clevis pin 504 moving in the clevis slot 508 of the hinge plate 416 pivots the hinge plate 416 about the hinge pin 512 (e.g., pivot axis) in this state moving the hinge pinch protrusion 516 into contact with the multiple tubes 124 pinching the multiple tubes 124 between the hinge pinch protrusion 516 of the hinge plate 416 and the platen pinch protrusion 520 of the clamp platen 420. When the translation rod 412 is extended in this state, fluid flow through the multiple tubes 124 is occluded completely at the pinched tubing point 328.

[0073] Fig. 5B shows a schematic plan view of the multi-tubing pinch valve assembly 400 in a partially closed or partially open, tubing-partially-occluded, state in accordance with examples of the present disclosure. In this tubing-partially-occluded state, the translation rod 412 of the linear actuator 410 is retracted by a second translation vector 502B from the position shown in Fig. 5A. As the translation rod 412 retracts, the clevis pin 504 moves in the clevis slot 508 of the hinge plate 416 pivoting the hinge plate 416 about the hinge pin 512 (e.g., pivot axis) in a first hinge plate rotation 506 A. In this position, the hinge pinch protrusion 516 is in contact with the multiple tubes 124 partially deforming the multiple tubes 124 and the fluid flow lumens of each of the multiple tubes 124. More specifically, the multiple tubes 124 are captured between the hinge pinch protrusion 516 of the hinge plate 416 and the platen pinch protrusion 520 of the clamp platen 420 providing a partially occluded flow path 324 through the multiple tubes 124. In the tubing- partially-occluded state, fluid flow rate through the multiple tubes 124 disposed in the tube receiving aperture 128 may be reduced when compared to a tubing-open state.

[0074] Fig. 5C shows a schematic plan view of the multi-tubing pinch valve assembly 400 in an open, tubing-open, state in accordance with examples of the present disclosure. In this tubing- open state, the translation rod 412 of the linear actuator 410 is retracted by a third translation vector 502C from the position shown in Figs. 5A and 5B. As the translation rod 412 retracts, the clevis pin 504 moves in the clevis slot 508 of the hinge plate 416 pivoting the hinge plate 416 about the hinge pin 512 (e.g., pivot axis) in a second hinge plate rotation 506B. In this open position, the hinge pinch protrusion 516 is brought out of deforming contact with the multiple tubes 124. In some examples, the multiple tubes 124 may return to an undeformed state and the fluid flow lumens of each of the multiple tubes 124 may open in this state. When the multi-tubing pinch valve assembly 400 is in the tubing-open state, the multiple tubes 124 may be removed from, or loaded into, the clamp platen 420. While described as moving between a closed and an open state, it should be appreciated that the multi-tubing pinch valve assembly 400 may move between an open and closed state by reversing the order and movements described above.

[0075] Figs. 6A and 6B show perspective views of a multi-tubing pinch valve assembly 400 in an array configuration in accordance with examples of the present disclosure. As described above, the array of valves may comprise a plurality of linear actuators 410 and hinge plates 616 that are similar, if not identical, to the hinge plate 416 described above. In Figs. 6 A and 6B, the multitubing pinch valve assembly 400 and array may include two immediately adjacent valves that share a dual clamp platen 620. The dual clamp platen 620 may comprise a first platen pinch protrusion 520 extending from a first surface toward a first hinge plate 616 and a second platen pinch protrusion 520 extending from an opposite second surface toward a second hinge plate 616. Among other things, this arrangement may allow for a more compact arrangement of valves in the array of valves making up the multi-tubing pinch valve assembly 400.

[0076] Fig. 7 shows a perspective view of a multi-tubing pinch valve assembly 700 in an array configuration, for example, in a multiple-bioreactor system. In some examples, the multi-tubing pinch valve assembly 700 may correspond to the multi-tubing pinch valve assembly 400 described in Figs. 4A-6B. In Fig. 7, the bioreactor support frame 744 includes a plurality of recesses 142 configured to receive and hold a respective bioreactor 704A-704E. As shown in Fig. 7, the multitubing pinch valve assembly 700 may include five separate bioreactors 704A-704E. In this example, the multi-tubing pinch valve assembly 700 may include an array of valves comprising five separate valves, one valve per bioreactor 704A-704E. Each valve in the array of valves of the multi-tubing pinch valve assembly 700 may be configured to pinch multiple tubes 124 for each bioreactor 704A-704E. For instance, each bioreactor 704A-704E may include at least four tubes. A first pair of these four tubes may be associated with an intracapillary loop of a respective bioreactor 704A-704E and a second pair of these four tubes may be associated with the extracapillary loop of the respective bioreactor 704A-704E. Among other things, the multi-tubing pinch valve assembly 700 may allow independent opening and closing of the multiple tubes 124 for each bioreactor 704A-704E.

[0077] Fig. 8 is a schematic block diagram 800 of a hydraulic layout for a cell expansion system having a single bioreactor 704A in accordance with examples of the present disclosure. As shown, the bioreactor of Fig. 8 includes an intracapillary circulation (IC) loop 810 and an extracapillary circulation (EC) loop 812. In a single bioreactor these loops may be opened and closed by one or more valves. A cell inlet bag attachment point is at 820. A cell inlet line extends through a cell inlet valve 822 to an IC inlet pump 824. From the pump 824, the line extends to an air removal chamber/level detection 826. From the air removal chamber/level detection 826, the line branches off to waste 828 and to IC circulation valve 830. From valve 830 the line extends into the bioreactor 704A. From the bioreactor 704A, the line branches to waste 828, IC circulation pump 832, and to harvest 836. Harvest valve 892 controls flow to harvest 836. From pump 832, the line goes to IC inlet pressure sensor 834 and to the IC circulation valve 830.

[0078] A reagent attachment point is at 838. A regent line extends through a reagent valve 840 to the cell inlet line. An IC media attachment point is at 842. An IC media line extends through IC media valve 844 to the reagent line.

[0079] An EC media attachment point is at 850. From the EC media attachment point 850, an EC line extends through EC media valve 852. A wash connection point is at 854, and a wash valve is at 856. A distribution valve is along a line connecting the EC loop 812 and the IC loop 810. The EC loop 812 includes an EC inlet pump 870, which is downstream from an EC fluid detector 872. The EC loop 812 further includes a GTM chamber 880, an EC inlet pressure sensor 882, an EC circulation pump 884, and an EC outlet pressure sensor 886. An EC waste valve 888 is on an EC waste line extending to waste 828. An IC waste valve 890 and an IC outlet pressure sensor 894 are upstream of waste 828 on the IC side.

[0080] Fig. 9 is a schematic block diagram 900 of a hydraulic layout for a cell expansion system having multiple bioreactors and a multi-tubing pinch valve assembly 100, 400, 700 in accordance with examples of the present disclosure. As shown in the schematic block diagram 900, the tubing associated with the IC loop and the EC loop of each of the five bioreactors may be controlled by the valves in the valve array. These valves may correspond to any of valves shown in the multipletube pinch valve assemblies 100, 400, 700 described herein. In some examples, the valve array shown in the schematic block diagram 900 may correspond to the valve array 148A-148E described in conjunction with Figs. 1A-3C. In one example, each valve in the valve array shown in the schematic block diagram 900 may correspond to the hinge plate 416 and clamp platen 420 of the multi-tubing pinch valve assembly 400 described in conjunction with Figs. 4A-5C and/or the hinge plate 616 and dual clamp platen 620 shown in Figs. 6A and 6B.

[0081] Figs. 10-13 illustrate a multitube pinch valve assembly 1100 in accordance with the present disclosure. The assembly 1100 generally includes a base 1110 and a bioreactor support frame 1310. With particular reference to Figs. 11 and 12, the base 1110 includes a plurality of hinge plates 1112A-1112E. Any suitable number of the hinge plates may be included. In the example illustrated, five hinge plates 1112A, 1112B, 1112C, 1112D, and 1112E are included. Each one of the hinge plates 1112A-1112E includes a hinge pinch protrusion 1114. The hinge plates 1112A-1112E are each individually actuated by any suitable actuation mechanism. In the example illustrated, each one of the hinge plates 1112A-1112E is actuated by a different actuation mechanism 1120A, 1120B, 1120C, 1120D, 1120E respectively. Each one of the actuation mechanisms 1120A-1120E may include any suitable linear actuator, such as any suitable solenoid, linear stepper motor, pneumatic actuator, etc., for example.

[0082] The base 1110 further includes a plurality of support posts 1150. The support posts 1150 are configured to cooperate with the bioreactor support frame 1310 as explained below to support the bioreactor support frame 1310 on the base 1110 and over the hinge plates 1112A-1112E. A strap 1152 is included to hold the bioreactor support frame 1310 on the base 1110.

[0083] The multitube pinch valve assembly 1100 further includes a rocker assembly 1160. The rocker assembly 1160 includes a rocker rod 1162, which cooperates with the base 1110 at an aperture 1164 defined by the base 1110. The rocker assembly 1160 includes a motor configured to rotate the rocker rod 1162. The rocker rod 1162 is mounted to the base 1110 in any suitable manner such that rotation of the rocker rod 1162 rocks the base 1110 and the bioreactors 704 A- 704E mounted thereto. In come configurations, the rocker assembly 1160 may be configured to invert the bioreactors 704A-704E.

[0084] With particular reference to Fig. 13, the bioreactor support frame 1310 may be configured to support five bioreactors 704A, 704B, 704C, 704D, 704E, or any other suitable number of bioreactors. The number of bioreactors included with the bioreactor support frame 1310 typically equals, or is less than, the number of hinge plates 1112A-1112E. The support frame 1310 includes a tubing platen 1320. The tubing platen 1320 includes a plurality of tube receiving apertures 1322 configured to secure the tubes 124 of the bioreactors 704A-704E to the tubing platen 1320. The tubes 124 extend generally parallel to lengths of the bioreactors 704A- 704E. In some applications, the tubes 124 may extend perpendicular to the bioreactors 704A- 704E, or at any other suitable angle. [0085] The bioreactor support frame 1310 further includes a plurality of receptacles 1340, each of which are configured to cooperate with the support posts 1150 of the base 1110. The support posts 1150 support the bioreactor support frame 1310 on top of the base 1110 and position the support frame 1310 such that the tubes 124 are arranged opposite to, and extend perpendicularly across, the hinge pinch protrusions 1114 of the hinge plates 1112A-1112E. Actuation of the different hinge plates 1112A-1112E moves the hinge pinch protrusions 1114 towards tubes to pinch the tubes 124 and restrict fluid flow through the tubes 124 and to the bioreactors 704A-704E in generally the same manner illustrated in Figs. 5A, 5B, and 5C illustrating the multi-tubing pinch valve assembly 400. When actuated upward, the hinge pinch protrusions 1114 will contact the tubes 124 on either side of any of the receiving apertures 1322 to bend the tubes 124 into occlusion. Bending the tubes 124 into occlusion reduces the amount of pinch force required from the actuation mechanisms 1120A-1120E as compared to when the pinch protrusions 1114 directly contact an opposing platen pinch protrusion. In some applications, the tubing platen 1320 may include platen pinch protrusions aligned with the hinge pinch protrusions 1114.

[0086] Figs. 14-18 illustrate an additional multitube pinch valve assembly 1500 in accordance with the present disclosure. The assembly 1500 generally includes a base 1510 and a bioreactor support frame 1710. With particular reference to Figs. 14 and 15, the base 1510 includes a latch 1512, which is rotatable between a closed position and an open position. In the open position, a rail 1520 of the base 1510 is accessible. The rail 1520 extends across the base 1510, and is configured to cooperate with the bioreactor support frame 1710, as explained herein. The rail 1520 may include a planar undersurface, or a pinch protrusion similar to the platen pinch protrusion 1330.

[0087] Opposite to the rail 1520, and seated within a housing 1530, are a plurality of actuation plates 1540A, 1540B, 1540C, 1540D, and 1540E. In the example illustrated, five actuation plates 1540A-1540E are included. The base 1510 may include any suitable number of actuation plates, however. Each one of the actuation plates 1540A-1540E includes a pinch protrusion 1542.

[0088] The actuation plates 1540A-1540E are individually actuatable by any suitable actuation mechanism. In the example illustrated, each one of the actuation plates 1540A-1540E is actuated by a different actuation mechanism 1550A, 1550B, 1550C, 1550D, 1550E respectively. The actuation mechanisms 1550A-1550E may include any suitable linear actuator, such as any suitable solenoid, linear stepper motor, pneumatic actuator, etc. The actuation mechanisms 1550A-1550E may be individually actuated to control fluid flow through tubes 124 of the bioreactors 704 A- 704E. [0089] With particular reference to FIG. 18, the bioreactor support frame 1710 includes a tubing platen 1730. The tubing platen 1730 defines a plurality of tube receiving apertures 1732 configured to secure the tubes 124 of the bioreactors 704A-704E to the tubing platen 1730. The tube receiving apertures 1732 are configured to support the tubes 124 so that the tubes 124 extend parallel to the lengths of the bioreactors 704A-704E.

[0090] The tubing platen 1730 further defines a center channel 1740 configured to receive the rail 1520 therein. At a rear of the center channel 1740 is a rear aperture 1742. The center channel 1740 extends perpendicular to the lengths of the bioreactors 704A-704E, and perpendicular to the tubes 124 supported by the tube receiving apertures 1732. At a rear end of the tubing platen 1730 are rear tabs 1750. At a front end of the tubing platen 1730 are front tabs 1752.

[0091] To connect the bioreactor support frame 1710 to the base 1510, the latch 1512 is folded downward, the tubing platen 1730 is positioned so that the rail 1520 enters through the rear aperture 1742, and then the tubing platen 1730 is slid onto the rail 1520 so that the rail 1520 extends along the center channel 1740. The tubing platen 1730 is slid along the rail 1520 until the rear tabs 1750 are seated within rear openings 1560 of the base 1510. The latch 1512 is then closed onto the front tabs 1752 so that the front tabs 1752 are seated within recesses 1562 defined on an inner surface of the latch 1512 to lock the tubing platen 1730 into position. The rear tabs 1750 and the front tabs 1752 improve alignment of the tubes 124 relative to the pinch protrusions 1542, and prevent loading the bioreactors backwards. Also, when the assembly 1500 includes a rocker, the tabs 1750, 1752 keep the bioreactors 704A-704E and tubes 124 in the correct position relative to the rod 1520. The tubing platen 1730 is oriented so that the pinch protrusions 1542 are perpendicular to the tubes 124. Actuation of the different actuation plates 1540A-1540E moves the pinch protrusions 1542 towards and into the center channel 1740 to pinch the tubes 124 between the pinch protrusions 1542 and the rail 1520 to restrict fluid flow through the tubes 124 in generally the same manner illustrated in Figs. 5A, 5B, and 5C illustrating the multi-tubing pinch valve assembly 400. In some applications, there may be a material between the tubes 124 and the rail 1520. The actuation plates 1540A-1540E may be individually actuated by the actuation mechanisms 1550A-1550E to selectively pinch the tubes 124, which selectively controls flow through the different tubes 124 and through the different bioreactors 704A-704E.

[0092] While the operations and steps above have been discussed and illustrated in relation to a particular sequence of events, it should be appreciated that changes, additions, and omissions to this sequence can occur without materially affecting the operation of the disclosed embodiments, configuration, and aspects. [0093] The exemplary systems and methods of this disclosure have been described in relation to pinch valves and multiple-tubing systems. However, to avoid unnecessarily obscuring the present disclosure, the preceding description omits a number of known structures and devices. This omission is not to be construed as a limitation of the scope of the claimed disclosure. Specific details are set forth to provide an understanding of the present disclosure. It should, however, be appreciated that the present disclosure may be practiced in a variety of ways beyond the specific detail set forth herein.

[0094] A number of variations and modifications of the disclosure can be used. It would be possible to provide for some features of the disclosure without providing others.

[0095] References in the specification to “one embodiment,” “an embodiment,” “an example embodiment,” “some embodiments,” etc., indicate that the embodiment described may include a particular feature, structure, or characteristic, but every embodiment may not necessarily include the particular feature, structure, or characteristic. Moreover, such phrases are not necessarily referring to the same embodiment. Further, when a particular feature, structure, or characteristic is described in conjunction with one embodiment, it is submitted that the description of such feature, structure, or characteristic may apply to any other embodiment unless so stated and/or except as will be readily apparent to one skilled in the art from the description. The present disclosure, in various embodiments, configurations, and aspects, includes components, methods, processes, systems and/or apparatus substantially as depicted and described herein, including various embodiments, subcombinations, and subsets thereof. Those of skill in the art will understand how to make and use the systems and methods disclosed herein after understanding the present disclosure. The present disclosure, in various embodiments, configurations, and aspects, includes providing devices and processes in the absence of items not depicted and/or described herein or in various embodiments, configurations, or aspects hereof, including in the absence of such items as may have been used in previous devices or processes, e.g., for improving performance, achieving ease, and/or reducing cost of implementation.

[0096] The foregoing discussion of the disclosure has been presented for purposes of illustration and description. The foregoing is not intended to limit the disclosure to the form or forms disclosed herein. In the foregoing Detailed Description for example, various features of the disclosure are grouped together in one or more embodiments, configurations, or aspects for the purpose of streamlining the disclosure. The features of the embodiments, configurations, or aspects of the disclosure may be combined in alternate embodiments, configurations, or aspects other than those discussed above. This method of disclosure is not to be interpreted as reflecting an intention that the claimed disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed embodiment, configuration, or aspect. Thus, the following claims are hereby incorporated into this Detailed Description, with each claim standing on its own as a separate preferred embodiment of the disclosure.

[0097] Moreover, though the description of the disclosure has included description of one or more embodiments, configurations, or aspects and certain variations and modifications, other variations, combinations, and modifications are within the scope of the disclosure, e.g., as may be within the skill and knowledge of those in the art, after understanding the present disclosure. It is intended to obtain rights, which include alternative embodiments, configurations, or aspects to the extent permitted, including alternate, interchangeable and/or equivalent structures, functions, ranges, or steps to those claimed, whether or not such alternate, interchangeable and/or equivalent structures, functions, ranges, or steps are disclosed herein, and without intending to publicly dedicate any patentable subject matter.

[0098] Exemplary aspects are directed to a multiple-tube pinch valve assembly, comprising: a camshaft extending a length along a longitudinal axis of the camshaft, the camshaft comprising at least one cam disposed along the length, the at least one cam comprising a cam profile shape defined around the longitudinal axis and a periphery of the camshaft and; a platen offset a distance from the longitudinal axis, the distance at least partially defining a space configured to receive multiple fluid flow tubes; and an anvil assembly comprising a cam contact portion disposed on a first side of the anvil assembly and a pinch edge disposed on a second side of the anvil assembly, wherein the anvil assembly is moveable between a retracted state such that the pinch edge is disposed outside of the space and an extended state such that the pinch edge is disposed inside the space adjacent the platen, and wherein the anvil assembly moves between the retracted state and the extended state by a rotation of the camshaft and the at least one cam.

[0099] Any one or more of the above aspects include wherein the cam profile shape comprises at least one cam lobe and at least one cam heel, wherein the anvil assembly is in the extended state when the cam lobe is disposed in contact with the cam contact portion, and wherein the anvil assembly is in the retracted state when the cam heel is disposed in contact with the cam contact portion. Any one or more of the above aspects include wherein the cam contact portion comprises at least one cam follower. Any one or more of the above aspects include wherein the space comprises a plurality of tube receiving apertures arranged side-by-side along a direction that is parallel to the longitudinal axis of the camshaft. Any one or more of the above aspects include wherein each tube receiving aperture of the plurality of tube receiving apertures extend in a direction that is perpendicular to the longitudinal axis of the camshaft. Any one or more of the above aspects further comprising: a plurality of tubes removably engaged with the plurality of tube receiving apertures such that a section of each tube of the plurality of tubes is disposed in the space adjacent the platen, wherein, in the retracted state, a lumen of each tube of the plurality of tubes is open along the section of each tube of the plurality of tubes, and wherein, in the extended state, the lumen of each tube of the plurality of tubes is closed at a point along the section of each tube of the plurality of tubes. Any one or more of the above aspects further comprising: a motor comprising an output shaft; a drive body attached to the output shaft of the motor; and a cam drive body attached to the camshaft, wherein rotation of the output shaft of the motor transmits power from the drive body to the cam drive body causing the camshaft to rotate relative to the anvil assembly. Any one or more of the above aspects further comprising: a support frame comprising a plurality of recesses, wherein each recess of the plurality of recesses is configured to hold a respective bioreactor, and wherein each tube of the plurality of tubes is associated with the respective bioreactor. Any one or more of the above aspects include wherein the anvil assembly, when moving between the retracted state and the extended state, translates along a plane that is perpendicular to a plane running through the plurality of tubes, and wherein, in the extended state, the pinch edge of the anvil assembly contacts and closes each tube of the plurality of tubes. Any one or more of the above aspects further comprising: an array of valves disposed along a length of the longitudinal axis of the camshaft, wherein each valve in the array of valves comprises a plurality of tube receiving apertures, and wherein each valve in the array of valves comprises a respective anvil assembly that is capable of moving between the retracted state and the extended state by the rotation of the camshaft and the at least one cam. Any one or more of the above aspects include wherein the respective anvil assembly of each valve in the array of valves is capable of moving between the retracted state and the extended state based on a position of a respective cam connected to the camshaft. Any one or more of the above aspects include wherein a first valve in the array of valves is capable of closing tubing contained within a first set of tube receiving apertures associated with the first valve at a same time or a different time as a second valve in the array of valves closes tubing contained within a second set of tube receiving apertures associated with the second valve. Any one or more of the above aspects include wherein, at a first time as the camshaft rotates, a first valve in the array of valves closes tubing contained within a first set of tube receiving apertures associated with the first valve, wherein, at a second time as the camshaft rotates, a second valve in the array of valves closes tubing contained within a second set of tube receiving apertures associated with the second valve. Any one or more of the above aspects include wherein, at a third time as the camshaft rotates, a third valve in the array of valves closes tubing contained within a third set of tube receiving apertures associated with the third valve, wherein, at a fourth time as the camshaft rotates, a fourth valve in the array of valves closes tubing contained within a fourth set of tube receiving apertures associated with the fourth valve, and wherein at a fifth time as the camshaft rotates, a fifth valve in the array of valves closes tubing contained within a fifth set of tube receiving apertures associated with the fifth valve. Any one or more of the above aspects include wherein the first set of tube receiving apertures, the second set of tube receiving apertures, the third set of tube receiving apertures, the fourth set of tube receiving apertures, and the fifth set of tube receiving apertures each comprise four tube receiving apertures. [0100] Exemplary aspects are directed to a multiple-tube pinch valve assembly, comprising: a motor comprising an output shaft; a drive body attached to the output shaft of the motor; a camshaft extending a length along a longitudinal axis of the camshaft, the camshaft comprising at least one cam disposed along the length, the at least one cam comprising a cam profile shape defined around the longitudinal axis and a periphery of the camshaft and; a cam drive body attached to the camshaft; a platen offset a distance from the longitudinal axis, the distance at least partially defining a space configured to receive multiple fluid flow tubes; and an anvil assembly comprising at least one cam follower disposed on a first side of the anvil assembly and a pinch edge disposed on a second side of the anvil assembly, wherein rotation of the output shaft of the motor transmits power from the drive body to the cam drive body causing the camshaft to rotate relative to the anvil assembly and move the anvil assembly between a retracted state, such that the pinch edge is disposed outside of the space, and an extended state, such that the pinch edge is disposed inside the space adjacent the platen, and wherein the anvil assembly moves between the retracted state and the extended state by rotation of the camshaft and the at least one cam.

[0101] Exemplary aspects are directed to a multi-tubing pinch valve assembly, comprising: a hinge plate extending a length from a first point to a second point, the hinge plate comprising: a pivot axis disposed adjacent the first point of the hinge plate; and a pinch protrusion disposed between the first point and the second point, the pinch protrusion extending from a surface of the hinge plate; a platen comprising a body offset a distance from the hinge plate, the platen comprising a tubing contact portion disposed on a surface of the platen; an actuator operatively connected to the hinge plate adjacent the second point, wherein the actuator is moveable between a retracted state and an extended state, wherein, in the retracted state, the hinge plate is pivoted about the pivot axis such that the second point of the hinge plate is separated from the platen by a first distance, wherein, in the extended state, the hinge plate is pivoted about the pivot axis such that the second point of the hinge plate is separated from the platen by a second distance, and wherein the first distance is greater than the second distance. [0102] Any one or more of the above aspects include wherein, in the extended state, the pinch protrusion of the hinge plate is adjacent the tubing contact portion of the platen, and wherein in the retracted state, the pinch protrusion of the hinge plate is offset from the tubing contact portion of the platen creating a receiving space between the hinge plate and the platen. Any one or more of the above aspects include wherein, in the retracted state, the pinch protrusion of the hinge plate is offset from the tubing contact portion of the platen by a first dimension defining a receiving space capable of receiving a plurality of tubes. Any one or more of the above aspects include wherein, in the extended state, the pinch protrusion of the hinge plate is offset from the tubing contact portion of the platen by a second dimension defining a pinched receiving space that is sized to pinch the plurality of tubes disposed between the hinge plate and the platen. Any one or more of the above aspects further comprising the plurality of tubes disposed in between the hinge plate and the platen, wherein the plurality of tubes provide fluid flow paths therethrough when in the retracted state, and wherein the fluid flow paths are restricted by the pinch protrusion of the hinge plate and the tubing contact portion of the platen when in the extended state. Any one or more of the above aspects include wherein the pinch protrusion of the hinge plate and the tubing contact portion of the platen completely restrict fluid flow in the plurality of tubes when in the extended state. Any one or more of the above aspects further comprising: a clevis disposed adjacent the second point of the hinge plate; and a clevis pin engaged with the clevis, the clevis pin being connected to an end of a translation rod of the actuator. Any one or more of the above aspects include wherein the tubing contact portion comprises a platen pinch protrusion extending from a surface of the platen, and wherein the surface of the platen faces the hinge plate. Any one or more of the above aspects include wherein the pivot axis is defined by a hinge pin engaged with the hinge plate and a hinge support block. Any one or more of the above aspects include wherein the actuator is one of a solenoid, a screw-type actuator, and a stepper motor actuated screw. Any one or more of the above aspects include wherein the clevis pin engages with a slot in the clevis. [0103] Exemplary aspects are directed to a multi-tubing pinch valve assembly, comprising: an array of valves disposed adjacent one another, wherein each valve in the array of valves comprises: a hinge plate extending a length from a first point to a second point, the hinge plate comprising: a pivot axis disposed adjacent the first point of the hinge plate; and a pinch protrusion disposed between the first point and the second point, the pinch protrusion extending from a surface of the hinge plate; a tubing contact body offset a distance from the hinge plate; an actuator operatively connected to the hinge plate adjacent the second point, wherein the actuator is moveable between a retracted state and an extended state, wherein, in the retracted state, the hinge plate is pivoted about the pivot axis such that the second point of the hinge plate is separated from the tubing contact body by a first distance, wherein, in the extended state, the hinge plate is pivoted about the pivot axis such that the second point of the hinge plate is separated from the tubing contact body by a second distance, and wherein the first distance is greater than the second distance.

[0104] Any one or more of the above aspects include wherein, in the extended state, the pinch protrusion of the hinge plate is adjacent the tubing contact body, and wherein in the retracted state, the pinch protrusion of the hinge plate is offset from the tubing contact body creating a receiving space between the hinge plate and the tubing contact body. Any one or more of the above aspects include wherein each valve in the array of valves is configured to receive a plurality of tubes in the receiving space. Any one or more of the above aspects include wherein each valve in the array of valves is configured to pinch the plurality of tubes in the receiving space in the extended state of the actuator. Any one or more of the above aspects include wherein each valve in the array of valves is independently operable such that each hinge plate of each valve is moveable between the retracted state and the extended state independently. Any one or more of the above aspects include wherein a first valve in the array of valves is operable together with and/or separately from a second valve in the array of valves. Any one or more of the above aspects further comprising: a support frame comprising a plurality of recesses, wherein each recess of the plurality of recesses is configured to hold a respective bioreactor, and wherein each tube of the plurality of tubes is associated with the respective bioreactor.

[0105] Exemplary aspects are directed to a multiple-bioreactor assembly comprising the multitubing pinch valve assembly of any of the above aspects.

[0106] Any one or more of the above aspects/embodiments as substantially disclosed herein.

[0107] Any one or more of the aspects/embodiments as substantially disclosed herein optionally in combination with any one or more other aspects/embodiments as substantially disclosed herein. [0108] One or means adapted to perform any one or more of the above aspects/embodiments as substantially disclosed herein.

[0109] Any one or more of the features disclosed herein.

[0110] Any one or more of the features as substantially disclosed herein.

[0111] Any one or more of the features as substantially disclosed herein in combination with any one or more other features as substantially disclosed herein.

[0112] Any one of the aspects/features/embodiments in combination with any one or more other aspects/features/embodiments .

[0113] Use of any one or more of the aspects or features as disclosed herein. [0114] It is to be appreciated that any feature described herein can be claimed in combination with any other feature(s) as described herein, regardless of whether the features come from the same described embodiment.

[0115] As used herein, the singular forms “a,” “an,” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “include,” “including,” “includes,” “comprise,” “comprises,” and/or “comprising,” when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The term “and/or” includes any and all combinations of one or more of the associated listed items.

[0116] The term “a” or “an” entity refers to one or more of that entity. As such, the terms “a” (or “an”), “one or more,” and “at least one” can be used interchangeably herein. It is also to be noted that the terms “comprising,” “including,” and “having” can be used interchangeably.

[0117] The phrases “at least one,” “one or more,” “or,” and “and/or” are open-ended expressions that are both conjunctive and disjunctive in operation. For example, each of the expressions “at least one of A, B and C,” “at least one of A, B, or C,” “one or more of A, B, and C,” “one or more of A, B, or C,” and “A, B, and/or C” means A alone, B alone, C alone, A and B together, A and C together, B and C together, or A, B, and C together. When each one of A, B, and C in the above expressions refers to an element, such as X, Y, and Z, or a class of elements, such as Xl-Xn, Y 1- Ym, and Zl-Zo, the phrase is intended to refer to a single element selected from X, Y, and Z, a combination of elements selected from the same class (e.g., XI and X2) as well as a combination of elements selected from two or more classes (e.g., Y 1 and Zo).

[0118] The term “automatic” and variations thereof, as used herein, refers to any process or operation, which is typically continuous or semi-continuous, done without material human input when the process or operation is performed. However, a process or operation can be automatic, even though performance of the process or operation uses material or immaterial human input, if the input is received before performance of the process or operation. Human input is deemed to be material if such input influences how the process or operation will be performed. Human input that consents to the performance of the process or operation is not deemed to be “material.” [0119] The terms “determine,” “calculate,” “compute,” and variations thereof, as used herein, are used interchangeably and include any type of methodology, process, mathematical operation, or technique.

[0120] Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and this disclosure.

[0121] It should be understood that every maximum numerical limitation given throughout this disclosure is deemed to include each and every lower numerical limitation as an alternative, as if such lower numerical limitations were expressly written herein. Every minimum numerical limitation given throughout this disclosure is deemed to include each and every higher numerical limitation as an alternative, as if such higher numerical limitations were expressly written herein. Every numerical range given throughout this disclosure is deemed to include each and every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein.

Claims

CLAIMS What is claimed is:

1. A multiple-tube pinch valve assembly comprising: a frame configured to hold a plurality of bioreactors and tubing connected to the bioreactors; a base configured to support the frame, the base including a plurality of pinch protrusions; and a plurality of actuators included with the base, each one of the plurality of actuators is connected to, and configured to actuate, a different one of the plurality of pinch protrusions; wherein: the frame and the base are configured to cooperate to retain the frame on the base and orient the frame such that the plurality of pinch protrusions are opposite to, and perpendicular to, the tubing connected to the bioreactors; and the plurality of actuators are configured to individually actuate each one of the plurality of pinch protrusions between a retracted position and an extended position, in the retracted position the pinch protrusions are spaced apart from the tubing, in the extended position the plurality of pinch protrusions pinch the tubing to restrict fluid flow through the tubing.

2. The multiple-tube pinch valve assembly of claim 1, wherein the plurality of actuators are linear actuators.

3. The multiple-tube pinch valve assembly of claim 1, further comprising a rocker assembly configured to rock the frame and the plurality of bioreactors.

4. The multiple-tube pinch valve assembly of claim 1, wherein in the retracted position the pinch protrusions are within a housing of the base, and in the extended position the pinch protrusions protrude out from within the housing.

5. The multiple-tube pinch valve assembly of claim 1, wherein the plurality of actuators are configured to individually actuate each one of the plurality of pinch protrusions to an intermediate position that is between the retracted position and the extended position, in the intermediate position the pinch protrusions pinch the tubing less than in the extended position.

6. The multiple-tube pinch valve assembly of claim 1, wherein the frame is configured to hold the tubing such that the tubing extends across the frame parallel to lengths of the plurality of bioreactors.

7. The multiple-tube pinch valve assembly of claim 1, further comprising a plurality of hinge plates, each one of the plurality of hinge plates includes one of the plurality of pinch protrusions; wherein the plurality of actuators are each connected to a first end of the plurality of hinge plates, and actuation of the plurality of hinge plates by the plurality of actuators pivots the plurality of hinge plates about a rotation axis at a second end of the plurality of hinge plates.

8. The multiple-tube pinch valve assembly of claim 1, wherein the frame includes a platen pinch protrusion opposite to the pinch protrusions such that in the extended position the pinch protrusions pinch the tubing against the platen pinch protrusion.

9. The multiple-tube pinch valve assembly of claim 1, wherein the pinch protrusions are oriented such that in the extended position the pinch protrusions bend the tubing.

10. The multiple-tube pinch valve assembly of claim 1, wherein: the base further includes a rail suspended over the pinch protrusions; and in the extended position the pinch protrusions pinch the tubing against the rail.

11. The multiple-tube pinch valve assembly of claim 10, wherein the frame is configured to slide onto the rail, the frame defining a channel configured to receive the rail therein.

12. A multiple-tube pinch valve assembly configured to cooperate with a frame holding a plurality of bioreactors and tubing connected to the bioreactors, the multiple-tube pinch valve assembly comprising: a plurality of hinge plates, each one of the plurality of hinge plates including a hinge pinch protrusion; a plurality of actuators, each one of the plurality of actuators is connected to, and configured to actuate, a different one of the plurality of hinge plates; and mounts configured to cooperate with the frame to support and orientate the frame such that the tubes are opposite to, and extend perpendicular to, the hinge pinch protrusions; wherein the plurality of actuators are configured to individually actuate each one of the plurality of hinge plates between a retracted position and an extended position, in the retracted position the hinge pinch protrusions are spaced apart from the tubing, in the extended position the hinge pinch protrusions pinch the tubing to restrict fluid flow through the tubing.

13. The multiple-tube pinch valve assembly of claim 12, wherein the mounts include mounting posts configured to cooperate with receptacles of the frame.

14. The multiple-tube pinch valve assembly of claim 12, further comprising a rocker assembly configured to rock the frame when the frame is in cooperation with the mounts.

15. The multiple-tube pinch valve assembly of claim 12, further comprising a housing including the plurality of hinge plates and the plurality of actuators; wherein in the retracted position the hinge pinch protrusions are within the housing, and in the extended position the hinge pinch protrusions protrude out from within the housing.

16. A multiple-tube pinch valve assembly configured to cooperate with a frame holding a plurality of bioreactors and tubing connected to the bioreactors, the multiple-tube pinch valve assembly comprising: a plurality of pinch protrusions; a plurality of actuators, each one of the plurality of actuators is connected to, and configured to actuate, a different one of the plurality of pinch protrusions; and a rail supported over the pinch protrusions, the rail configured to cooperate with the frame to support the frame over the pinch protrusions and orientate the frame such that the tubes are opposite to, and extend perpendicular to, the pinch protrusions; wherein the plurality of actuators are configured to individually actuate each one of the plurality of pinch protrusions between a retracted position and an extended position, in the retracted position the pinch protrusions are spaced apart from the tubing, in the extended position the pinch protrusions pinch the tubing against the rail to restrict fluid flow through the tubing.

17. The multiple-tube pinch valve assembly of claim 16, further comprising a housing including the plurality of pinch protrusions and the plurality of actuators; wherein in the retracted position the pinch protrusions are within the housing, and in the extended position the pinch protrusions protrude out from within the housing.

18. The multiple-tube pinch valve assembly of claim 16, further comprising the frame, the frame including a channel configured to cooperate with the rail.

19. The multiple-tube pinch valve assembly of claim 16, further comprising a latch, the latch movable between an open position and a closed position, in the open position the rail is accessible to allow the frame to slide onto the rail, and in the closed position the frame is locked onto the rail.

20. The multiple-tube pinch valve assembly of claim 16, wherein the plurality of actuators are linear actuators.