AR020076A1 - LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA. - Google Patents
LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA.Info
- Publication number
- AR020076A1 AR020076A1 ARP990102364A ARP990102364A AR020076A1 AR 020076 A1 AR020076 A1 AR 020076A1 AR P990102364 A ARP990102364 A AR P990102364A AR P990102364 A ARP990102364 A AR P990102364A AR 020076 A1 AR020076 A1 AR 020076A1
- Authority
- AR
- Argentina
- Prior art keywords
- production
- cell line
- genes
- retroviral vector
- packaging cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 230000001177 retroviral effect Effects 0.000 title abstract 4
- 239000013598 vector Substances 0.000 title abstract 3
- 238000004806 packaging method and process Methods 0.000 title abstract 2
- 241001430294 unidentified retrovirus Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000700605 Viruses Species 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 108700004025 env Genes Proteins 0.000 abstract 1
- 108700004026 gag Genes Proteins 0.000 abstract 1
- 108700004029 pol Genes Proteins 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 238000004448 titration Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
- C12N2740/13051—Methods of production or purification of viral material
- C12N2740/13052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Línea celular de empaquetamiento retroviral anfotropico que contiene genes funcionales gag, pol y env integrados en el genoma, donde la expresion de losgenes gag y pol está regulada independientemente de la expresion de los genes env, donde elgenoma de dicha línea celular contiene uno o dos genes envfuncionalmente activos para la obtencion de una titulacion de vectores retrovirales de al menos 107 de unidades que forman colonias /ml de sobrenadantes decultivo celular; proceso para suproduccion , virus vectorial retroviral incompetente a la replicacion y proceso para su produccion.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98109135 | 1998-05-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR020076A1 true AR020076A1 (es) | 2002-04-10 |
Family
ID=8231962
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990102364A AR020076A1 (es) | 1998-05-20 | 1999-05-18 | LíNEA CELULAR DE EMPAQUETAMIENTO DE RETROVIRUS ANFOTRoPICO, PROCESO PARA SU PRODUCCIoN, Y PROCESO PARA LA PRODUCCIoN DE UN VECTOR RETROVIRAL MEDIANTE LA MISMA. |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US6475786B1 (es) |
| EP (1) | EP1080219B1 (es) |
| JP (1) | JP4571307B2 (es) |
| KR (1) | KR100653154B1 (es) |
| CN (1) | CN1154743C (es) |
| AR (1) | AR020076A1 (es) |
| AT (1) | ATE378416T1 (es) |
| AU (1) | AU4261599A (es) |
| CA (1) | CA2331440C (es) |
| DE (1) | DE69937556T2 (es) |
| DK (1) | DK1080219T3 (es) |
| ES (1) | ES2296410T3 (es) |
| WO (1) | WO1999060143A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2425001A4 (en) | 2009-04-30 | 2012-11-14 | Univ California | ANTI-HIV COMBINATION VECTORS, TARGETING VECTORS AND METHOD OF USE |
| WO2014026053A1 (en) | 2012-08-09 | 2014-02-13 | The Regents Of The University Of California | Cd25 pre-selective combination anti-hiv vectors, targeting vectors, and methods of use |
| US20140065110A1 (en) | 2012-08-31 | 2014-03-06 | The Regents Of The University Of California | Genetically modified msc and therapeutic methods |
| WO2020172259A1 (en) | 2019-02-20 | 2020-08-27 | Research Institute At Nationwide Children's Hospital | Cancer-targeted, virus-encoded, regulatable t (catvert) or nk cell (catvern) linkers |
| US11912986B2 (en) | 2019-09-23 | 2024-02-27 | The Regents Of The University Of California | Methods for screening genetic perturbations |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU3069189A (en) * | 1988-02-05 | 1989-08-25 | Trustees Of Columbia University In The City Of New York, The | Retroviral packaging cell lines and processes of using same |
| US5591624A (en) * | 1988-03-21 | 1997-01-07 | Chiron Viagene, Inc. | Retroviral packaging cell lines |
-
1999
- 1999-05-14 US US09/700,979 patent/US6475786B1/en not_active Expired - Lifetime
- 1999-05-14 CA CA2331440A patent/CA2331440C/en not_active Expired - Lifetime
- 1999-05-14 EP EP99952099A patent/EP1080219B1/en not_active Expired - Lifetime
- 1999-05-14 DE DE69937556T patent/DE69937556T2/de not_active Expired - Lifetime
- 1999-05-14 ES ES99952099T patent/ES2296410T3/es not_active Expired - Lifetime
- 1999-05-14 WO PCT/EP1999/003304 patent/WO1999060143A1/en not_active Ceased
- 1999-05-14 JP JP2000549749A patent/JP4571307B2/ja not_active Expired - Lifetime
- 1999-05-14 AU AU42615/99A patent/AU4261599A/en not_active Abandoned
- 1999-05-14 CN CNB998060682A patent/CN1154743C/zh not_active Expired - Lifetime
- 1999-05-14 DK DK99952099T patent/DK1080219T3/da active
- 1999-05-14 AT AT99952099T patent/ATE378416T1/de active
- 1999-05-14 KR KR1020007013038A patent/KR100653154B1/ko not_active Expired - Lifetime
- 1999-05-18 AR ARP990102364A patent/AR020076A1/es active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| CN1300325A (zh) | 2001-06-20 |
| ATE378416T1 (de) | 2007-11-15 |
| KR20010071300A (ko) | 2001-07-28 |
| EP1080219A1 (en) | 2001-03-07 |
| DK1080219T3 (da) | 2008-03-03 |
| US6475786B1 (en) | 2002-11-05 |
| CN1154743C (zh) | 2004-06-23 |
| AU4261599A (en) | 1999-12-06 |
| JP2002515255A (ja) | 2002-05-28 |
| EP1080219B1 (en) | 2007-11-14 |
| DE69937556D1 (de) | 2007-12-27 |
| ES2296410T3 (es) | 2008-04-16 |
| KR100653154B1 (ko) | 2006-12-01 |
| CA2331440A1 (en) | 1999-11-25 |
| CA2331440C (en) | 2010-07-13 |
| DE69937556T2 (de) | 2008-10-23 |
| JP4571307B2 (ja) | 2010-10-27 |
| WO1999060143A1 (en) | 1999-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |