AR048191A1 - Expresion optimizada de hpv 52 l1 en levaduras - Google Patents

Expresion optimizada de hpv 52 l1 en levaduras

Info

Publication number
AR048191A1
AR048191A1 ARP050101174A ARP050101174A AR048191A1 AR 048191 A1 AR048191 A1 AR 048191A1 AR P050101174 A ARP050101174 A AR P050101174A AR P050101174 A ARP050101174 A AR P050101174A AR 048191 A1 AR048191 A1 AR 048191A1
Authority
AR
Argentina
Prior art keywords
hpv
vlps
synthetic
protein
produce
Prior art date
Application number
ARP050101174A
Other languages
English (en)
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Publication of AR048191A1 publication Critical patent/AR048191A1/es

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C12N7/045—Pseudoviral particles; Non infectious pseudovirions, e.g. genetically engineered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5258—Virus-like particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20023—Virus like particles [VLP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Se proporciona moléculas de DNA sintético que codifican la proteína HPV 52 L1. Específicamente, se proporciona polinucleotidos que codifican la proteína HPV 52 L1, donde dichos polinucleotidos están codon-optimizados para una alto nivel de expresion en una célula de levadura. Entre otros, la secuencia de nucleotidos de la molécula sintética se altera para eliminar las senales de terminacion de la transcripcion que son reconocidas por la levadura. Las moléculas sintéticas pueden ser utilizadas para producir partículas semejantes a virus (VLPs) HPV 52, y para producir vacunas y composiciones farmacéuticas que comprende las VLPs HPV 52. Dichas vacunas proporcionan una inmunoprofilaxis efectiva contra la infeccion de papilomavirus a través de la neutralizacion de anticuerpos e inmunidad mediada por células y puede también ser util para el tratamiento de las infecciones existentes de HPV.
ARP050101174A 2004-03-24 2005-03-23 Expresion optimizada de hpv 52 l1 en levaduras AR048191A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US55592604P 2004-03-24 2004-03-24

Publications (1)

Publication Number Publication Date
AR048191A1 true AR048191A1 (es) 2006-04-05

Family

ID=34964085

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050101174A AR048191A1 (es) 2004-03-24 2005-03-23 Expresion optimizada de hpv 52 l1 en levaduras

Country Status (31)

Country Link
US (2) US7700103B2 (es)
EP (1) EP1730175B1 (es)
JP (1) JP4833962B2 (es)
KR (1) KR101203403B1 (es)
CN (1) CN1934131B (es)
AR (1) AR048191A1 (es)
AT (1) ATE466022T1 (es)
AU (1) AU2005230907C1 (es)
BE (1) BE2015C066I2 (es)
BR (1) BRPI0509079B8 (es)
CA (1) CA2560487C (es)
CY (2) CY1110349T1 (es)
DE (1) DE602005020913D1 (es)
DK (1) DK1730175T3 (es)
ES (1) ES2343255T3 (es)
FR (1) FR15C0085I2 (es)
HU (1) HUS1500062I1 (es)
IL (1) IL178140A (es)
LT (2) LTPA2015050I1 (es)
LU (1) LU92903I2 (es)
MY (1) MY148656A (es)
NL (1) NL300777I1 (es)
NO (3) NO338055B1 (es)
NZ (1) NZ549898A (es)
PL (1) PL1730175T3 (es)
PT (1) PT1730175E (es)
RU (1) RU2373219C2 (es)
SI (1) SI1730175T1 (es)
TW (1) TWI349036B (es)
WO (1) WO2005097821A1 (es)
ZA (1) ZA200607575B (es)

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US7858098B2 (en) * 2002-12-20 2010-12-28 Glaxosmithkline Biologicals, S.A. Vaccine
CN1869215B (zh) * 2006-05-19 2012-07-04 长春百克生物科技股份公司 一种制备人乳头瘤病毒的病毒样颗粒的方法
CA2679720A1 (en) 2007-03-09 2008-09-18 Merck & Co., Inc. Papillomavirus vaccine compositions
EP2479187A1 (en) 2007-11-23 2012-07-25 Shanghai Zerun Biotechnology Co., Ltd. Genes encoding major capsid protein L1 of human papilloma viruses
CN101440371B (zh) * 2007-11-23 2012-09-05 上海泽润生物科技有限公司 一种16型人乳头状瘤病毒主要衣壳蛋白l1基因
AU2011252844B2 (en) * 2010-05-14 2014-07-03 Baxalta GmbH Chimeric OspA genes, proteins, and methods of use thereof
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
WO2012000454A1 (zh) * 2010-07-02 2012-01-05 厦门大学 截短的人乳头瘤病毒52型l1蛋白
US8361720B2 (en) * 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
CN103562392B (zh) 2010-12-03 2016-07-20 Ms技术有限责任公司 植物细胞中草甘膦抗性编码核酸分子的优化表达
CN102154325B (zh) * 2011-01-01 2013-08-21 上海生物制品研究所有限责任公司 针对人乳头瘤状病毒的疫苗及其制法和用途
RU2445357C1 (ru) * 2011-02-15 2012-03-20 Закрытое акционерное общество научно-производственная компания "Комбиотех" Рекомбинантный штамм дрожжей pichia angusta - продуцент капсидного белка l1 вируса папилломы человека типа 16
RU2445358C1 (ru) * 2011-02-15 2012-03-20 Закрытое акционерное общество научно-производственная компания "Комбиотех" Рекомбинантный штамм дрожжей pichia angusta - продуцент капсидного белка l1 вируса папилломы человека типа 18
CN104120089B (zh) * 2013-04-26 2019-09-24 北京安百胜生物科技有限公司 用汉逊酵母表达系统产生hpv52 l1蛋白的方法
CN110592133A (zh) * 2013-05-17 2019-12-20 北京安百胜生物科技有限公司 用汉逊酵母表达系统产生hpv31 l1蛋白的方法
EP3057612B1 (en) 2013-10-16 2020-05-06 Merck Sharp & Dohme Corp. Method of obtaining thermostable dried vaccine formulations
CN104878022A (zh) * 2015-04-27 2015-09-02 段青姣 编码hpv58l1、hpv52l1蛋白的核苷酸序列及其应用
CN106701796B (zh) * 2015-08-12 2021-11-16 北京康乐卫士生物技术股份有限公司 52型重组人乳头瘤病毒病毒样颗粒及其制备方法
CN109750049B (zh) * 2017-11-07 2023-08-18 上海泽润生物科技有限公司 重组人乳头瘤病毒52亚型蛋白表达
JP2023516904A (ja) 2020-02-14 2023-04-21 メルク・シャープ・アンド・ドーム・エルエルシー Ｈｐｖワクチン
CN114539365B (zh) * 2020-11-26 2023-12-01 中国医学科学院基础医学研究所 一种改造的人乳头瘤病毒52型l1蛋白及其用途
CN113073105B (zh) * 2021-03-23 2023-06-13 重庆博唯佰泰生物制药有限公司 一种表达hpv 56l1的多核苷酸序列及其表达载体、宿主细胞和应用
CN113549634B (zh) * 2021-06-07 2023-03-31 郑州大学 编码可溶性hpv58 l1蛋白的基因及其重组质粒的构建与应用
AR126708A1 (es) 2021-08-06 2023-11-08 Merck Sharp & Dohme Llc Vacuna para el vph
JP2024532127A (ja) 2021-08-19 2024-09-05 メルク・シャープ・アンド・ドーム・エルエルシー 熱安定性脂質ナノ粒子およびその使用方法
TW202513091A (zh) 2023-06-09 2025-04-01 美商默沙東有限責任公司 用於人類乳突病毒疫苗之奈米乳液佐劑組合物

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KR101080628B1 (ko) * 2003-03-24 2011-11-08 머크 샤프 앤드 돔 코포레이션 효모에서 ｈｐｖ 31 ｌ1의 최적 발현
MY140664A (en) * 2003-09-29 2010-01-15 Merck Sharp & Dohme Optimized expression of hpv 45 l1 in yeast
MY139500A (en) * 2003-11-12 2009-10-30 Merck Sharp & Dohme Optimized expression of hpv 58 l1 in yeast

Also Published As

Publication number Publication date
US7700103B2 (en) 2010-04-20
AU2005230907A1 (en) 2005-10-20
ZA200607575B (en) 2008-08-27
CY2015052I2 (el) 2016-06-22
IL178140A (en) 2010-11-30
US20100035818A1 (en) 2010-02-11
NL300777I2 (es) 2015-12-29
CA2560487A1 (en) 2005-10-20
BE2015C066I2 (es) 2024-08-08
TWI349036B (en) 2011-09-21
LTC1730175I2 (lt) 2017-05-10
NO2017005I1 (no) 2017-01-20
NO338055B1 (no) 2016-07-25
ATE466022T1 (de) 2010-05-15
DK1730175T3 (da) 2010-08-23
US20080226660A1 (en) 2008-09-18
EP1730175B1 (en) 2010-04-28
CY2015052I1 (el) 2016-06-22
BRPI0509079B1 (pt) 2020-05-19
SI1730175T1 (sl) 2010-08-31
US7744892B2 (en) 2010-06-29
RU2006137363A (ru) 2008-04-27
NZ549898A (en) 2009-06-26
CA2560487C (en) 2013-01-29
PL1730175T3 (pl) 2010-09-30
CN1934131B (zh) 2010-12-29
MY148656A (en) 2013-05-15
CN1934131A (zh) 2007-03-21
AU2005230907C1 (en) 2016-07-28
RU2373219C2 (ru) 2009-11-20
HUS1500062I1 (hu) 2017-07-28
NL300777I1 (es) 2015-12-29
LTPA2015050I1 (lt) 2016-01-11
ES2343255T3 (es) 2010-07-27
FR15C0085I2 (fr) 2016-11-25
IL178140A0 (en) 2006-12-31
BRPI0509079A (pt) 2007-08-21
DE602005020913D1 (de) 2010-06-10
FR15C0085I1 (es) 2016-08-01
EP1730175A1 (en) 2006-12-13
NO2022050I1 (no) 2022-11-30
WO2005097821A1 (en) 2005-10-20
KR101203403B1 (ko) 2012-11-21
TW200602490A (en) 2006-01-16
JP4833962B2 (ja) 2011-12-07
PT1730175E (pt) 2010-06-25
KR20060134120A (ko) 2006-12-27
BRPI0509079B8 (pt) 2021-07-06
CY1110349T1 (el) 2015-04-29
NO20064815L (no) 2006-10-23
AU2005230907B2 (en) 2011-05-19
LU92903I2 (fr) 2016-03-08
JP2007530040A (ja) 2007-11-01

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