AR065533A1 - Metodos de transcripcion, constructos de adn de repeticion en tandem, productores de proteinas que atacan geminivirus patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales de los agentes patogenos - Google Patents

Metodos de transcripcion, constructos de adn de repeticion en tandem, productores de proteinas que atacan geminivirus patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales de los agentes patogenos

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AR065533A1
AR065533A1 ARP080102219A ARP080102219A AR065533A1 AR 065533 A1 AR065533 A1 AR 065533A1 AR P080102219 A ARP080102219 A AR P080102219A AR P080102219 A ARP080102219 A AR P080102219A AR 065533 A1 AR065533 A1 AR 065533A1
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Argentina
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group
trs
plants
zfp
derivatives
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ARP080102219A
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Fernandez Pol Jose Alberto
Fernandez Pol Sebastian
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    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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Abstract

Métodos y composiciones referidas al tratamiento, al control o la inhibicion de Geminivirus, mediante la reduccion o la inhibicion del crecimiento de los Geminivirus (GV) que emplean un compuesto que tiene la estructura siguiente (1), o una salfarmacéuticamente aceptables de ese compuesto, caracterizado porque a R1, R2, R3 y R4 se seleccionan del grupo consistente en un grupo carboxilo, un grupo metilo, un grupo etilo, un grupo propilo, un grupo isopropilo, un grupo butilo, un grupoisobutilo, un grupo butilo secundario, un grupo butilo terciario, un grupo pentilo, un grupo isopentilo, un grupo neopentilo, fluor, cloro, bromo, yodo y hidrogeno. Se brinda varias clases de compuestos antivirosicos que se puede utilizar odesintegrar geminivirus, tales como los geminivirus africanos del mosaico de la casava (ACMV) y los geminivirus del Este de África del mosaico de la casava (EAMGV), a través del ataque del dominio de dedos de zinc [Zn2+]-[CCHC] o de variaciones deél, el tal dominio estando presente en la nucelocápside u otras ZFP virosicas. A los agentes antivirosicos se los diseno para usárselos en plantas infectadas por virus patogenos. Estos agentes antivirosicos comprenden el Ácido Picolínico (PA) yderivados de él. Los agentes pueden ligar de manera coordinada el Zn2+ a la proteína del virus y expulsar el Zn2+ de la ZFP (proteína dedo de Zinc) o se pueden ligar al Zn2+ y formar un complejo ternario con la ZFP [PA-Zn2+-ZFP] virosica. Además,también se desarrollo un sistema disenado para detectar e identificar derivados antivirosicos de ácido picolínico contra los GV, basado sobre la capacidad de estos derivados para reaccionar e interrumpir los motivos de CCHC (o una variacion deellos) en la ZFP de los GV. Los agentes y métodos antivirosicos que comprenden el empleo de ácido picolínico, ácido fusárico y derivados de estos agregados en forma exogena, también se pueden emplear para prevenir, controlar o eliminar cualquierotrovirus de plantas que contenga ZFP esenciales en el proteoma o que utilice las ZFP de la célula hospedante para la activacion transcripcional. Además, se da a conocer una tecnología de Secuencia Repetida de Tandem (TRS), denotada como método deConstructo de TRS en caja, para la produccion de plantas singénicas con resistencia aumentada a los virus. Esta tecnología incorpora, en el genoma de la planta, secuencias de ADN estables repetidas en tandem, que codifican enzimas que puedenproducir PA o derivados de él. La produccion aumentada de PA o derivados de él. La produccion aumentada de PA endogeno por la Carboxilasa de Ácido Picolínico, inducida por un promotor de proteína virosica por TRS que está presente en el Constructode TRS en caja, puede inactivar e interrumpir la ZFP virosicas necesarias para la replicacion de los virus. Las plantas singénicas en las que se introdujo el Constructo de TRS en caja para conferir resistencia a los virus son genéticamente establesy fértiles. Las plantas singénicas con TRS producen flores y semillas que se puede volver a plantar para producir nuevas plantas singénicas con TRS que son resistentes a los virus. Por anadidura, las plantas singénicas con TRS están sujetas a laspresiones evolutivas del ambiente, tal como si fueran la contraparte de tipo silvestre. Más aun; dado que no contienen agentes agregados en forma exogena o endogena, son comestibles sin que haya que hacerles más modificaciones. Reivindicacion 1: Unmétodo de transcripcion, caracterizado porque comprende transcribir genes letales que precisan una secuencia específica de promotor inducible por ADN, que está presente en la unidad de caja que tiene elevada afinidad por un (unos) factor(es) detrascripcion virosica, la secuencia de ADN del promotor está presente en sentido ascendente respecto de los genes letales.
ARP080102219A 2008-03-19 2008-05-27 Metodos de transcripcion, constructos de adn de repeticion en tandem, productores de proteinas que atacan geminivirus patogenos de plantas a traves de la interrupcion de los factores de transcripcion esenciales de los agentes patogenos AR065533A1 (es)

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US12/051,080 US20090241222A1 (en) 2008-03-19 2008-03-19 Tandem reapeat dna constructs producing proteins that attack plant pathogenic viruses, fungi, and bacteria by disrupting transcription factors essential for replication thereof in plants

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AR065533A1 true AR065533A1 (es) 2009-06-17

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109964278A (zh) * 2017-03-30 2019-07-02 艾腾怀斯股份有限公司 通过并行评估分类器输出校正第一分类器中的误差的系统和方法

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* Cited by examiner, † Cited by third party
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CA2795480C (en) 2010-04-06 2021-10-26 Vrije Universiteit Brussel Specific delivery of agrochemicals
WO2017136895A1 (en) * 2016-02-10 2017-08-17 Queensland University Of Technology Constructs and methods for conferring virus resistance
WO2021209275A1 (en) * 2020-04-12 2021-10-21 Novoscope Ip Limited Particles, dna & rna
US12268676B2 (en) 2021-01-20 2025-04-08 Indian Institute Of Science Antiviral applications of picolinic acid and its derivatives
CN112899301B (zh) * 2021-01-29 2023-04-11 中国热带农业科学院热带生物技术研究所 一种木薯普通花叶病毒诱导的基因沉默系统及其应用
US20240307514A1 (en) * 2021-07-02 2024-09-19 Dignity Health Modulation of acmsd protein expression

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109964278A (zh) * 2017-03-30 2019-07-02 艾腾怀斯股份有限公司 通过并行评估分类器输出校正第一分类器中的误差的系统和方法
CN109964278B (zh) * 2017-03-30 2023-06-27 艾腾怀斯股份有限公司 通过并行评估分类器输出校正第一分类器中的误差

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