AR088332A1 - Dispositivo y metodo balanceados para la produccion de productos microbianos - Google Patents

Dispositivo y metodo balanceados para la produccion de productos microbianos

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Publication number
AR088332A1
AR088332A1 ARP120103822A AR088332A1 AR 088332 A1 AR088332 A1 AR 088332A1 AR P120103822 A ARP120103822 A AR P120103822A AR 088332 A1 AR088332 A1 AR 088332A1
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Argentina
Prior art keywords
phase
phase space
organic material
group
packed bed
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English (en)
Inventor
R Coyne John
Lamontagne Michael
P Lanciault David
N Ames Robert
Chisolm Robert
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Tenfold Technologies Llc
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Publication date
Application filed by Tenfold Technologies Llc filed Critical Tenfold Technologies Llc
Publication of AR088332A1 publication Critical patent/AR088332A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C12M25/18—Fixed or packed bed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/16—Sterilization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/141—Feedstock
    • Y02P20/145—Feedstock the feedstock being materials of biological origin
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00—Technologies for wastewater treatment
    • Y02W10/30—Wastewater or sewage treatment systems using renewable energies
    • Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Computer Hardware Design (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

Reivindicación 1: Un método multifase para procesar material orgánico en menos de 15 días que comprende: (a) una primera fase donde dicho material orgánico se hidroliza; (b) una segunda fase superpuesta con dicha primera fase donde dicho material hidrolizado de (a) se somete a acidogénesis y acetogénesis para obtener un material que comprende hidrógeno, dióxido de carbono, ácidos orgánicos volátiles y precursores metanogénicos; (c) una tercera fase superpuesta con dicha segunda fase donde dichos precursores metanogénicos en el material en (b) se convierten en metano y dicho material en (b) se somete en forma adicional a desnitrificación; y (d) una cuarta fase superpuesta con dicha tercera fase donde dicho material de (c) se somete a estabilización y reversión. Reivindicación 5: El método de acuerdo con la reivindicación 1, que además comprende proporcionar un fluido de trabajo a cada fase. Reivindicación 6: El método de acuerdo con la reivindicación 5, donde dicho fluido de trabajo proporcionado a la primera fase es material orgánico, donde dicho material orgánico es una carga de alimentación, agua y una levadura seca activa. Reivindicación 7: El método de acuerdo con la reivindicación 1, donde dicho material orgánico se procesa para obtener un bioestimulante, un corrector de suelos o un aditivo de suelos. Reivindicación 10: Un dispositivo biorreactor multifase para la producción de un producto que comprende un consorcio microbiano de material orgánico que comprende (a) un espacio de primera fase para la hidrólisis de material orgánico; (b) un espacio de segunda fase para la acidogénesis/acetogénesis de material orgánico del espacio de primera fase, donde dicho espacio de segunda fase se superpone con el espacio de primera fase de (a); (c) un espacio de tercera fase para la metanogénesis de material orgánico del espacio de segunda fase donde dicho espacio de tercera fase se superpone del espacio de segunda fase de (b); (d) un espacio de cuarta fase para la estabilización y reversión del material orgánico de (c), donde dicho espacio de cuarta fase se superpone con el espacio de tercera fase de (c). Reivindicación 15: El dispositivo biorreactor multifase de la reivindicación 10 donde cada espacio de fase comprende una pluralidad de microorganismos y donde (a) el espacio de primera fase comprende microorganismos que tienen al menos una de las funciones metabólicas seleccionadas del grupo que consiste en: metanógeno, reductores de azufre, declorinación oxidación de hierro, nitrificación y degradación aromática; (b) el espacio de segunda fase comprende microorganismos que tienen al menos una de las funciones metabólicas seleccionadas del grupo que consiste en: metanógeno, declorinación, degradación aromática, denitrificador, nitrificación, anamox, CO₂ alto; (c) el espacio de tercera fase comprende microorganismos que tienen al menos una de las funciones metabólicas seleccionadas del grupo que consiste en: degradación aromática, denitrificador, nitrificación, heterotrofia, nitrificación, metanotrofia, y CO₂ alto; (d) el espacio de cuarta fase comprende microorganismos que tienen al menos una de las funciones metabólicas seleccionadas del grupo que consiste en: metanógeno, degradación aromática, denitriticador, nitrificación, heterotrofia, nitrificación, anaerobia, oxidación de azufre, anamox, CO₂ alto, y oxidación de hierro. Reivindicación 17: El dispositivo reactor multifase de la reivindicación 10, donde dicho dispositivo además comprende (a) un tanque de contención, conectado al espacio de primera fase, para mezclar el fluido de trabajo y pasar el fluido de trabajo al espacio de primera fase a una velocidad de carga; (b) un pasteurizador conectado al espacio de cuarta fase; y (c) un concentrador conectado al pasteurizador. Reivindicación 28: Un biorreactor multifase que comprende un dispositivo de contención que comprende: (a) un tanque de contención para mezclar fluido de trabajo; (b) un reactor de mezcla completa conectado al tanque de contención para recircular fluido de trabajo; (c) un clarificador conectado al reactor de mezcla completa para separar sólidos del fluido de trabajo; (d) cuatro o más reactores de lecho empacados para hidrólisis, acidogénesis, acetogénesis, metanogénesis, estabilización y reconstitución de material orgánico, donde cada reactor de lecho empacado comprende medios fijos, donde cada reactor de lecho empacado está en comunicación fluida con al menos el reactor de lecho empacado adyacente y donde el clarificador conectado al reactor de mezcla completa está en comunicación fluida con un primer reactor de lecho empacado, un reactor de lecho empacado adyacente a dicho reactor de mezcla completa con un clarificador, donde el medio fijado se asegura al interior de cada reactor empacado; y (e) un contenedor para la deposición del material orgánico procesado de dichos reactores y operativamente conectado a un reactor de lecho empacado final, un reactor de lecho empacado final que es al menos un cuarto reactor de lecho empacado. Reivindicación 32: El biorreactor multifase de acuerdo con la reivindicación 28, donde dicho biorreactor además comprende (a) un grupo de controles de dispositivo operativamente acoplado al dispositivo de contención; (b) un grupo de sensores de dispositivo operativamente acoplado al dispositivo de contención; (c) un grupo de grupos de datos de fase, derivado del grupo de sensores de dispositivo; (d) un grupo de perfiles de fase. Reivindicación 32: Un método para producir un resultado microbiano de un material orgánico que comprende aplicar material orgánico y fluido de trabajo al biorreactor de la reivindicación 28 bajo condiciones suficientes para producir dicho resultado microbiano en menos que aproximadamente 15 días. Reivindicación 33: El método de acuerdo con la reivindicación 32, donde dicho resultado microbiano es un aditivo o corrector de suelos. Reivindicación 34: Un corrector de suelos obtenido por el proceso de la reivindicación 6, 31 ó 33. Reivindicación 35: Un corrector de suelos que tiene las siguientes características: (a) tiene un pH de aproximadamente 7,5 a 8; (b) un rango de COD menor que aproximadamente 150 mg/L; (c) un rango de conductividad de aproximadamente 600 uS a 1400 uS; (d) un color ámbar claro entre aproximadamente 500 pt/co unidades a aproximadamente 700 pt/co unidades en una escala platino a cobalto (pt/co); (e) comprende Syntrophus, Desulfovibrio, Symbiobacteria, Georgfuschia, Thauera, Nitrosomonas, Bellilinea, Sulfuritalea y Owenweeksia; (f) tiene tina biomasa mayor que 10⁷ microbios por ml.; (g) contiene entre aproximadamente 10 - 60 ng/mI de ADN.
ARP120103822 2011-10-13 2012-10-12 Dispositivo y metodo balanceados para la produccion de productos microbianos AR088332A1 (es)

Applications Claiming Priority (2)

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US201161627633P 2011-10-13 2011-10-13
US201161581679P 2011-12-30 2011-12-30

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AR088332A1 true AR088332A1 (es) 2014-05-28

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US (4) US11999940B2 (es)
EP (1) EP2766489B1 (es)
JP (1) JP5944513B2 (es)
AR (1) AR088332A1 (es)
AU (1) AU2012322016B2 (es)
BR (1) BR112014008931B1 (es)
CA (2) CA2851282C (es)
ES (1) ES2976908T3 (es)
HU (1) HUE066545T2 (es)
IN (1) IN2014MN00874A (es)
LT (1) LT2766489T (es)
MX (1) MX354929B (es)
PL (1) PL2766489T3 (es)
PT (1) PT2766489T (es)
WO (1) WO2013056084A2 (es)
ZA (1) ZA201402935B (es)

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