AR097977A1 - Método de producción de l-aminoácidos - Google Patents
Método de producción de l-aminoácidosInfo
- Publication number
- AR097977A1 AR097977A1 ARP140103766A ARP140103766A AR097977A1 AR 097977 A1 AR097977 A1 AR 097977A1 AR P140103766 A ARP140103766 A AR P140103766A AR P140103766 A ARP140103766 A AR P140103766A AR 097977 A1 AR097977 A1 AR 097977A1
- Authority
- AR
- Argentina
- Prior art keywords
- amino acids
- producing
- target gene
- recombinant coryneform
- coryneform microorganism
- Prior art date
Links
- 150000008575 L-amino acids Chemical class 0.000 title abstract 5
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 244000005700 microbiome Species 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 108020004705 Codon Proteins 0.000 abstract 1
- 150000001242 acetic acid derivatives Chemical class 0.000 abstract 1
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y403/00—Carbon-nitrogen lyases (4.3)
- C12Y403/03—Amine-lyases (4.3.3)
- C12Y403/03007—4-Hydroxy-tetrahydrodipicolinate synthase (4.3.3.7)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Se proporciona un método de producción de L-aminoácidos mediante el uso de un microorganismo corineforme recombinante en el que la expresión de un gen objetivo es debilitada mediante el uso de un método de inhibición de la transcripción génica. Reivindicación 1: Un método de producción de L-aminoácidos, comprendiendo el método 1) cultivar un microorganismo corineforme recombinante capaz de producir L-aminoácidos, en donde el microorganismo corineforme recombinante se transforma mediante inserción de un promotor inducible por acetato corriente abajo de un codón de terminación de un gen objetivo en un cromosoma; y 2) agregar acetatos durante el cultivo para debilitar la expresión del gen objetivo y para fortalecer la capacidad de producción de L-aminoácidos del microorganismo corineforme recombinante.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20130121090 | 2013-10-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR097977A1 true AR097977A1 (es) | 2016-04-20 |
Family
ID=53035683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP140103766A AR097977A1 (es) | 2013-10-11 | 2014-10-09 | Método de producción de l-aminoácidos |
Country Status (16)
| Country | Link |
|---|---|
| JP (3) | JP6291042B2 (es) |
| KR (1) | KR101518860B1 (es) |
| CN (1) | CN104561163B (es) |
| AR (1) | AR097977A1 (es) |
| AU (2) | AU2014332732B2 (es) |
| BR (1) | BR112016008029A2 (es) |
| CA (1) | CA2925659C (es) |
| DK (1) | DK2860256T3 (es) |
| ES (1) | ES2626048T3 (es) |
| GE (2) | GEAP201814125A (es) |
| JO (1) | JO3255B1 (es) |
| MY (1) | MY172463A (es) |
| PH (1) | PH12016500640B1 (es) |
| PL (1) | PL2860256T3 (es) |
| RU (2) | RU2614258C2 (es) |
| UA (1) | UA117935C2 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
| CN105734004B (zh) * | 2016-03-02 | 2020-06-19 | 廊坊梅花生物技术开发有限公司 | 重组菌株及其制备方法、应用 |
| KR101925930B1 (ko) * | 2017-07-24 | 2019-02-26 | 씨제이제일제당 주식회사 | Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법 |
| BR112020004575B1 (pt) | 2018-03-09 | 2023-03-14 | Cj Cheiljedang Corporation | Polinucleotídeo, vetor, microrganismo do gênero corynebacterium, métodos para produção de substância-alvo e para preparar uma composição fermentada. |
| KR102221040B1 (ko) * | 2019-05-09 | 2021-03-03 | 씨제이제일제당 주식회사 | L-아미노산을 생산하는 미생물 및 이를 이용한 l-아미노산을 생산하는 방법 |
| KR102134418B1 (ko) * | 2019-06-17 | 2020-07-16 | 씨제이제일제당 주식회사 | L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법 |
| KR102207867B1 (ko) * | 2020-01-21 | 2021-01-26 | 씨제이제일제당 주식회사 | Nadp 의존적 글리세르알데하이드-3-포스페이트 디하이드로지나제를 포함하는 미생물을 이용하여 ｌ-아미노산을 생산하는 방법 |
| KR102527895B1 (ko) * | 2021-01-11 | 2023-04-28 | 씨제이제일제당 (주) | GlxR 단백질 변이체 또는 이를 이용한 쓰레오닌 생산방법 |
| CN115678933A (zh) * | 2022-09-26 | 2023-02-03 | 廊坊梅花生物技术开发有限公司 | 一种提高赖氨酸发酵转化率的方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6649379B1 (en) * | 1987-06-12 | 2003-11-18 | Massachusetts Institute Of Technology | Method and deregulated enzyme for threonine production |
| JP3036912B2 (ja) * | 1991-09-02 | 2000-04-24 | 協和醗酵工業株式会社 | 遺伝子発現調節ｄｎａ |
| CN1117152C (zh) * | 1994-03-04 | 2003-08-06 | 味之素株式会社 | 生产l－赖氨酸的方法 |
| SK285013B6 (sk) * | 1995-06-07 | 2006-04-06 | Ajinomoto Co., Inc. | Rekombinantná DNA, autonómne replikovateľná v bunkách koryneformných baktérií, koryneformná baktéria a spôsob výroby L-lyzínu |
| EP1029919B1 (de) * | 1999-02-20 | 2004-03-31 | Degussa AG | Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien |
| KR20020073153A (ko) * | 1999-12-24 | 2002-09-19 | 아지노모토 가부시키가이샤 | Ｌ-아미노산의 제조법 및 신규 유전자 |
| WO2002040679A2 (en) * | 2000-11-15 | 2002-05-23 | Archer-Daniels-Midland Company | Corynebacterium glutamicum promoters |
| KR100397423B1 (ko) * | 2001-02-13 | 2003-09-13 | 씨제이 주식회사 | Ｌ-쓰레오닌의 제조방법 |
| DE10224088A1 (de) * | 2002-05-31 | 2003-12-11 | Degussa | Verfahren zur Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien die ein abgeschwächtes mez-Gen enthalten |
| JP2004261150A (ja) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | 目的物質の製造法 |
| JP2005006564A (ja) | 2003-06-19 | 2005-01-13 | Japan Tobacco Inc | 硝酸イオン誘導性プロモーター |
| JP2010226956A (ja) * | 2007-07-23 | 2010-10-14 | Ajinomoto Co Inc | Ｌ−リジンの製造法 |
| KR101335853B1 (ko) | 2011-12-01 | 2013-12-02 | 씨제이제일제당 (주) | Ｌ-아미노산 및 리보플라빈을 동시에 생산하는 미생물 및 이를 이용한 ｌ-아미노산 및 리보플라빈을 생산하는 방법 |
| KR101518860B1 (ko) * | 2013-10-11 | 2015-05-12 | 씨제이제일제당 (주) | L-아미노산의 생산 방법 |
-
2014
- 2014-07-18 KR KR1020140091307A patent/KR101518860B1/ko active Active
- 2014-08-10 UA UAA201603717A patent/UA117935C2/uk unknown
- 2014-09-24 PL PL14186238T patent/PL2860256T3/pl unknown
- 2014-09-24 ES ES14186238.3T patent/ES2626048T3/es active Active
- 2014-09-24 DK DK14186238.3T patent/DK2860256T3/en active
- 2014-10-08 MY MYPI2016701087A patent/MY172463A/en unknown
- 2014-10-08 JP JP2016521791A patent/JP6291042B2/ja active Active
- 2014-10-08 RU RU2015151124A patent/RU2614258C2/ru active
- 2014-10-08 BR BR112016008029A patent/BR112016008029A2/pt not_active Application Discontinuation
- 2014-10-08 GE GEAP201814125A patent/GEAP201814125A/en unknown
- 2014-10-08 AU AU2014332732A patent/AU2014332732B2/en active Active
- 2014-10-08 CA CA2925659A patent/CA2925659C/en active Active
- 2014-10-08 GE GEAP201414125A patent/GEP20196943B/en unknown
- 2014-10-08 RU RU2016110804A patent/RU2651461C2/ru active
- 2014-10-09 JO JOP/2014/0287A patent/JO3255B1/ar active
- 2014-10-09 AR ARP140103766A patent/AR097977A1/es unknown
- 2014-10-11 CN CN201410654216.XA patent/CN104561163B/zh active Active
-
2016
- 2016-04-07 PH PH12016500640A patent/PH12016500640B1/en unknown
-
2017
- 2017-12-07 AU AU2017272295A patent/AU2017272295B2/en active Active
-
2018
- 2018-02-08 JP JP2018021175A patent/JP6824918B2/ja active Active
-
2020
- 2020-01-24 JP JP2020009563A patent/JP2020078312A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JP6824918B2 (ja) | 2021-02-03 |
| UA117935C2 (uk) | 2018-10-25 |
| RU2015151124A (ru) | 2016-06-27 |
| KR20150042692A (ko) | 2015-04-21 |
| ES2626048T3 (es) | 2017-07-21 |
| JP2018113966A (ja) | 2018-07-26 |
| RU2614258C2 (ru) | 2017-03-24 |
| RU2651461C2 (ru) | 2018-04-19 |
| DK2860256T3 (en) | 2017-06-06 |
| AU2014332732A1 (en) | 2016-04-21 |
| JO3255B1 (ar) | 2018-09-16 |
| JP2016532439A (ja) | 2016-10-20 |
| MY172463A (en) | 2019-11-26 |
| BR112016008029A2 (pt) | 2017-10-03 |
| CN104561163A (zh) | 2015-04-29 |
| GEAP201814125A (en) | 2018-10-10 |
| PH12016500640A1 (en) | 2016-05-30 |
| AU2017272295B2 (en) | 2019-05-16 |
| JP2020078312A (ja) | 2020-05-28 |
| PH12016500640B1 (en) | 2016-05-30 |
| AU2014332732B2 (en) | 2017-12-07 |
| CN104561163B (zh) | 2018-11-06 |
| KR101518860B1 (ko) | 2015-05-12 |
| PL2860256T3 (pl) | 2017-08-31 |
| AU2017272295A1 (en) | 2018-01-04 |
| RU2016110804A (ru) | 2016-07-27 |
| CA2925659C (en) | 2018-10-23 |
| CA2925659A1 (en) | 2015-04-16 |
| GEP20196943B (en) | 2019-02-11 |
| JP6291042B2 (ja) | 2018-03-14 |
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