AR102734A1 - Proteínas inhibidoras de insectos - Google Patents
Proteínas inhibidoras de insectosInfo
- Publication number
- AR102734A1 AR102734A1 ARP150103795A ARP150103795A AR102734A1 AR 102734 A1 AR102734 A1 AR 102734A1 AR P150103795 A ARP150103795 A AR P150103795A AR P150103795 A ARP150103795 A AR P150103795A AR 102734 A1 AR102734 A1 AR 102734A1
- Authority
- AR
- Argentina
- Prior art keywords
- seq
- group
- sequence selected
- polynucleotide
- recombinant
- Prior art date
Links
- 241000238631 Hexapoda Species 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000002401 inhibitory effect Effects 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 abstract 8
- 102000040430 polynucleotide Human genes 0.000 abstract 8
- 108091033319 polynucleotide Proteins 0.000 abstract 8
- 239000003112 inhibitor Substances 0.000 abstract 4
- 239000002773 nucleotide Substances 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 241000254173 Coleoptera Species 0.000 abstract 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 230000000749 insecticidal effect Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 206010061217 Infestation Diseases 0.000 abstract 1
- 241000255777 Lepidoptera Species 0.000 abstract 1
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
- Virology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Se proporcionan plantas transgénicas, células de plantas, semillas y partes de plantas resistentes a una infestación de lepidópteros y coleópteros, las cuales contienen secuencias de polinucleótidos que codifican proteínas insecticidas de la presente. Se proporcionan, además, métodos para detectar la presencia de los polinucleótidos o las proteínas de la presente en una muestra biológica y métodos de control de plagas de coleópteros y lepidópteros utilizando cualquiera de las proteínas insecticidas de tipo TIC3668. Reivindicación 1: Una molécula aislada de polinucleótido recombinante que codifique un polipéptido inhibidor de insectos que contenga una secuencia de aminoácidos seleccionada del grupo que consta de SEC ID Nº 25, SEC ID Nº 23, SEC ID Nº 24, SEC ID Nº 26, SEC ID Nº 27, SEC ID Nº 28, SEC ID Nº 29, SEC ID Nº 30 y SEC ID Nº 31. Reivindicación 2: Que la molécula aislada de polinucleótido recombinante del reclamo 1 contenga: a) una secuencia de nucleótidos seleccionada del grupo que consta de SEC ID Nº 37, SEC ID Nº 33, SEC ID Nº 35, SEC ID Nº 39, SEC ID Nº 41, SEC ID Nº 43, SEC ID Nº 45, SEC ID Nº 47, SEC ID Nº 49, SEC ID Nº 64, SEC ID Nº 65, SEC ID Nº 66, SEC ID Nº 67, SEC ID Nº 68, SEC ID Nº 69, SEC ID Nº 70, SEC ID Nº 71 y SEC ID Nº 72 o (b) una secuencia de nucleótidos que se hibride en condiciones rigurosas al: (i) el complemento inverso de la secuencia de nucleótidos desde la posición 4 a la 885 de una secuencia seleccionada del grupo que consta de SEC ID Nº 37, SEC ID Nº 33, SEC ID Nº 35, SEC ID Nº 39, SEC ID Nº 41, SEC ID Nº 43, SEC ID Nº 45, SEC ID Nº 47, SEC ID Nº 49, SEC ID Nº 64, SEC ID Nº 65, SEC ID Nº 66, SEC ID Nº 67, SEC ID Nº 68, SEC ID Nº 69, SEC ID Nº 70, SEC ID Nº 71 y SEC ID Nº 72 o (ii) el complemento inverso a una secuencia seleccionada del grupo conste de SEC ID Nº 52, SEC ID Nº 53, SEC ID Nº 54, SEC ID Nº 55, SEC ID Nº 56, SEC ID Nº 57, SEC ID Nº 58, SEC ID Nº 59, SEC ID Nº 60 y SEC ID Nº 61; que dichas condiciones rigurosas comprendan la hibridación durante 4 a 12 horas en formamida al 50%, 1 M de NaCl y 1% de SDS a 37ºC y un lavado en 0,1 X SSC entre 60ºC y 65ºC. Reivindicación 4: Un polipéptido recombinante inhibidor de insectos codificado por la molécula de polinucleótidos del reclamo 1. Reivindicación 11: Una composición inhibidora de insectos que contenga la molécula aislada recombinante de polinucleótidos del reclamo 1. Reivindicación 18: Un producto de consumo que contenga a la célula huésped del reclamo 10, dicho producto comprenda una cantidad detectable del polinucleótido recombinante aislado o un polipéptido recombinante inhibidor de insectos codificado por un polinucleótido recombinante aislado.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462082504P | 2014-11-20 | 2014-11-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR102734A1 true AR102734A1 (es) | 2017-03-22 |
Family
ID=54754818
Family Applications (8)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP150103795A AR102734A1 (es) | 2014-11-20 | 2015-11-20 | Proteínas inhibidoras de insectos |
| ARP200103456A AR120734A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103459A AR120737A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103453A AR120731A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103460A AR120738A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103451A AR120729A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103457A AR120735A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103458A AR120736A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
Family Applications After (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP200103456A AR120734A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103459A AR120737A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103453A AR120731A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103460A AR120738A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103451A AR120729A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103457A AR120735A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
| ARP200103458A AR120736A2 (es) | 2014-11-20 | 2020-12-11 | Proteínas inhibidoras de insectos |
Country Status (21)
| Country | Link |
|---|---|
| US (3) | US10662439B2 (es) |
| EP (2) | EP3926049A1 (es) |
| JP (1) | JP6731408B2 (es) |
| CN (3) | CN112626088B (es) |
| AR (8) | AR102734A1 (es) |
| BR (7) | BR122020006737B1 (es) |
| CA (3) | CA3297448A1 (es) |
| CL (1) | CL2017001298A1 (es) |
| CO (1) | CO2017005958A2 (es) |
| CR (1) | CR20170255A (es) |
| EC (1) | ECSP17038086A (es) |
| ES (1) | ES2861596T3 (es) |
| MX (3) | MX386168B (es) |
| NI (1) | NI201700059A (es) |
| PE (2) | PE20221408A1 (es) |
| PY (1) | PY1572044A (es) |
| RU (1) | RU2740313C2 (es) |
| SV (1) | SV2017005446A (es) |
| UA (1) | UA125491C2 (es) |
| UY (1) | UY36407A (es) |
| WO (1) | WO2016081618A1 (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11130964B2 (en) | 2014-11-20 | 2021-09-28 | Monsanto Technology Llc | Insect inhibitory proteins |
| EP3926049A1 (en) | 2014-11-20 | 2021-12-22 | Monsanto Technology LLC | Novel insect inhibitory proteins |
| MX387077B (es) | 2016-05-04 | 2025-03-19 | Pioneer Hi Bred Int | Proteinas insecticidas y metodos para sus usos. |
| US10626151B2 (en) * | 2016-10-10 | 2020-04-21 | Monsanto Technology Llc | Insect inhibitory proteins |
| CN106591352B (zh) * | 2016-11-21 | 2020-05-05 | 北京大北农科技集团股份有限公司 | 杀虫蛋白组合及其管理昆虫抗性的方法 |
| AR111398A1 (es) * | 2017-04-03 | 2019-07-10 | Monsanto Technology Llc | Proteínas inhibidoras de insectos |
| US10743535B2 (en) | 2017-08-18 | 2020-08-18 | H&K Solutions Llc | Insecticide for flight-capable pests |
| CN110029172B (zh) * | 2018-12-28 | 2021-07-09 | 华中农业大学 | 马、驴源性成分二重pcr检测试剂盒 |
| CR20220534A (es) | 2020-04-24 | 2023-02-17 | Monsanto Technology Llc | Evento de maíz transgénico mon95275, métodos para su detección y usos de este |
| CN116253779B (zh) * | 2021-12-13 | 2025-11-25 | 北京大北农生物技术有限公司 | 杀虫蛋白的用途 |
| CN114317578A (zh) * | 2021-12-13 | 2022-04-12 | 北京大北农生物技术有限公司 | 杀虫蛋白的用途 |
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| US6501009B1 (en) | 1999-08-19 | 2002-12-31 | Monsanto Technology Llc | Expression of Cry3B insecticidal protein in plants |
| US6706860B2 (en) * | 2000-05-18 | 2004-03-16 | Bayer Bioscience N.V. | Toxins |
| US6551962B1 (en) | 2000-10-06 | 2003-04-22 | Monsanto Technology Llc | Method for deploying a transgenic refuge |
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| US9593345B2 (en) * | 2012-10-15 | 2017-03-14 | Pioneer Hi-Bred International, Inc. | Bacillus thuringiensis gene with coleopteran activity |
| EP3926049A1 (en) | 2014-11-20 | 2021-12-22 | Monsanto Technology LLC | Novel insect inhibitory proteins |
| US11130964B2 (en) | 2014-11-20 | 2021-09-28 | Monsanto Technology Llc | Insect inhibitory proteins |
| US10626151B2 (en) * | 2016-10-10 | 2020-04-21 | Monsanto Technology Llc | Insect inhibitory proteins |
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