AT230021B - Process for the biological production of streptokinase - Google Patents

Process for the biological production of streptokinase

Info

Publication number
AT230021B
AT230021B AT970960A AT970960A AT230021B AT 230021 B AT230021 B AT 230021B AT 970960 A AT970960 A AT 970960A AT 970960 A AT970960 A AT 970960A AT 230021 B AT230021 B AT 230021B
Authority
AT
Austria
Prior art keywords
streptokinase
biological production
preculture
strain
culture
Prior art date
Application number
AT970960A
Other languages
German (de)
Original Assignee
Behringwerke Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Behringwerke Ag filed Critical Behringwerke Ag
Application granted granted Critical
Publication of AT230021B publication Critical patent/AT230021B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/46—Streptococcus ; Enterococcus; Lactococcus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

  

   <Desc/Clms Page number 1> 
 



  Verfahren zur biologischen Gewinnung von Streptokinase 
 EMI1.1 
 

 <Desc/Clms Page number 2> 

 



   Das Kurvenblatt zeigt das Ergebnis des   erfindungsgemässenverfahrens   in graphischer Darstellung. Die Kurve I ergab sich aus   den Trübungswerten   oder sogenannten optischen Dichten (OD) in der Vorkultur nach Verdünnung 1 : 5. Die Kurve II stellt die optimalen Streptokinase-Werte verschiedener Hauptkulturen dar, die zu entsprechenden Zeiten mit der Vorkultur beimpft waren. 



   Mit Hilfe dieser Methode der rechtzeitigen Übertragung der Vorkultur auf die Hauptkultur gelingt es also, maximale Streptokinasemengen zu erhalten. 



   Beispiel : Streptokokken vomstreptokinasebildenden Stamm H 46A werden in Blutagarröhrchen bei   37 C   gezüchtet. Nach 24 h werden von 4 Röhrchen die vermehrten Keime mit einer durch pankreatische Verdauung von Rinderfleisch hergestellten Bouillon (Methode Pope,   C. G.   und M. L. Smith, J.   Path. Bact.   
 EMI2.1 
 zeichnete Lösung wurde in 80   l   Müller-Medium   (whys,     techn.   Rep. Ser. 61 [1953], S. 46), das   noch 2%   Glucose enthält, gegeben, bei   370C   erwärmt und unter vorsichtigem, gegebenenfalls automatischen, mit einem Autotitrator   auszuführendem Nachtitrieren   mit   ln-Natronlauge   auf einem pH-Wert von 7,0 bis 7, 8 gehalten.

   Die so behandelte Kulturlösung lieferte eine Streptokinaseausbeute von 620   Einheiten/ml   Kul-   turflüssigkeit.  



   <Desc / Clms Page number 1>
 



  Process for the biological production of streptokinase
 EMI1.1
 

 <Desc / Clms Page number 2>

 



   The graph shows the result of the method according to the invention in a graphic representation. Curve I resulted from the turbidity values or so-called optical densities (OD) in the preculture after dilution 1: 5. Curve II represents the optimal streptokinase values of various main cultures which were inoculated with the preculture at the appropriate times.



   With the help of this method of timely transfer of the preculture to the main culture, it is possible to obtain maximum amounts of streptokinase.



   Example: Streptococci from the streptokinase-producing strain H 46A are grown in blood agar tubes at 37 ° C. After 24 hours, the multiplied germs are removed from 4 tubes with a bouillon produced by pancreatic digestion of beef (Pope method, C. G. and M. L. Smith, J. Path. Bact.
 EMI2.1
 The solution drawn was added to 80 l Müller medium (whys, techn. Rep. Ser. 61 [1953], p. 46), which still contains 2% glucose, heated at 37 ° C. and carefully, if necessary automatically, with an autotitrator titration to be carried out with 1N sodium hydroxide solution kept at a pH of 7.0 to 7.8.

   The culture solution treated in this way gave a streptokinase yield of 620 units / ml culture fluid.

Claims (1)

PATENTANSPRUCH : Verfahren zur biologischen Gewinnung besonders hoher Streptokinasemengen unter Verwendung eines an sich bekannten streptokinasebildenden Streptokokkenstammes, wie Stamm H 46 A, dadurch gekennzeichnet, dass man zur Beimpfung der Hauptkultur eine etwa 7-9 h alte Vorkultur, deren optische Dichte EMI2.2 PATENT CLAIM: Process for the biological recovery of particularly high amounts of streptokinase using a streptokinase-forming streptococcal strain known per se, such as strain H 46 A, characterized in that an approximately 7-9 h old preculture is used for inoculating the main culture, its optical density EMI2.2
AT970960A 1959-12-29 1960-12-27 Process for the biological production of streptokinase AT230021B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE230021T 1959-12-29

Publications (1)

Publication Number Publication Date
AT230021B true AT230021B (en) 1963-11-11

Family

ID=29721504

Family Applications (1)

Application Number Title Priority Date Filing Date
AT970960A AT230021B (en) 1959-12-29 1960-12-27 Process for the biological production of streptokinase

Country Status (1)

Country Link
AT (1) AT230021B (en)

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