AT244357B - Process for obtaining pharmaceutically acceptable globulin - Google Patents
Process for obtaining pharmaceutically acceptable globulinInfo
- Publication number
- AT244357B AT244357B AT42861A AT42861A AT244357B AT 244357 B AT244357 B AT 244357B AT 42861 A AT42861 A AT 42861A AT 42861 A AT42861 A AT 42861A AT 244357 B AT244357 B AT 244357B
- Authority
- AT
- Austria
- Prior art keywords
- globulin
- pharmaceutically acceptable
- obtaining pharmaceutically
- mixed
- separated
- Prior art date
Links
- 102000006395 Globulins Human genes 0.000 title claims description 10
- 108010044091 Globulins Proteins 0.000 title claims description 10
- 238000000034 method Methods 0.000 title claims description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000002244 precipitate Substances 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 230000006920 protein precipitation Effects 0.000 claims description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000005686 Serum Globulins Human genes 0.000 description 1
- 108010045362 Serum Globulins Proteins 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/08—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from eggs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Description
<Desc/Clms Page number 1>
Verfahren zur Gewinnung von pharmazeutisch verwendbarem Globulin
Es ist bereits bekannt, Globulin aus proteinhaltigen Lösungen durch Fällung mit Magnesiumsulfat oder Ammoniumsulfat abzuscheiden, doch ist es schwierig, mit Hilfe dieses Verfahrens ein reines pharmazeutisch verwendbares Globulin zu erhalten,. da die Reste des Füllungsmittels schwer zu entfernen sind und umständliche Reinigungsoperationen, wie z. B. eine Dialyse, erfordern. Es ist weiterhin bekannt, dass man bei der Gewinnung von Serumalbumin das Serumglobulin aus dem mit Wasser verdünnten Blutserum durch Einleiten von Kohlensäure abscheiden kann.
Es wurde nun gefunden, dass man aus dem Eiklar, insbesondere aus Hühnereiern, ein reines, pharmazeutisch verwendbares Globulin in einfacher Weise und mit geringen Herstellungskosten erhalten kann,
EMI1.1
mitvermischt und der gebildete Niederschlag abgetrennt wird, worauf aus dem Filtrat das Globulin in für die Eiweissfällung an sich bekannter Weise mittels C02 ausgeschieden und in üblicher Weise isoliert wird.
Das folgende Ausführungsbeispiel erläutert die Erfindung : Eiklar wird im Verhältnis von 1 Vol.-Teil zu 1, 5 Vol.-Teilen 0, Seiger Kochsalzlösung vermischt, dann wird zentrifugiert und das Filtrat im Ver-
EMI1.2
Kohlendioxyd eingeleitet, der Niederschlag einige Zeit absetzen gelassen und sodann das Globulin durch Ausschleudern abgetrennt, mit Wasser und schonend getrocknet, bis man das reine, pharmazeutisch verwendbare Globulin als trockenes Pulver erhält.
**WARNUNG** Ende DESC Feld kannt Anfang CLMS uberlappen**.
<Desc / Clms Page number 1>
Process for obtaining pharmaceutically acceptable globulin
It is already known to separate globulin from protein-containing solutions by precipitation with magnesium sulfate or ammonium sulfate, but it is difficult to obtain a pure pharmaceutically usable globulin with the aid of this method. since the remnants of the filling agent are difficult to remove and cumbersome cleaning operations, such as. B. dialysis require. It is also known that when serum albumin is obtained, the serum globulin can be separated from the blood serum diluted with water by introducing carbonic acid.
It has now been found that a pure, pharmaceutically usable globulin can be obtained in a simple manner and at low production costs from egg white, in particular from chicken eggs,
EMI1.1
mixed and the precipitate formed is separated off, whereupon the globulin is excreted from the filtrate in a manner known per se for protein precipitation by means of CO 2 and isolated in the usual way.
The following exemplary embodiment explains the invention: Egg white is mixed in a ratio of 1 part by volume to 1.5 parts by volume of 0 Seiger saline solution, then it is centrifuged and the filtrate is mixed
EMI1.2
Carbon dioxide was introduced, the precipitate was allowed to settle for some time and the globulin was then separated off by centrifugation, dried gently with water until the pure, pharmaceutically acceptable globulin was obtained as a dry powder.
** WARNING ** End of DESC field may overlap beginning of CLMS **.
Claims (1)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BE244357X | 1960-06-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AT244357B true AT244357B (en) | 1966-01-10 |
Family
ID=3866468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT42861A AT244357B (en) | 1960-06-29 | 1961-01-19 | Process for obtaining pharmaceutically acceptable globulin |
Country Status (1)
| Country | Link |
|---|---|
| AT (1) | AT244357B (en) |
-
1961
- 1961-01-19 AT AT42861A patent/AT244357B/en active
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