AT512453A5 - Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv - Google Patents

Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv Download PDF

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Publication number
AT512453A5
AT512453A5 AT94962010A AT94962010A AT512453A5 AT 512453 A5 AT512453 A5 AT 512453A5 AT 94962010 A AT94962010 A AT 94962010A AT 94962010 A AT94962010 A AT 94962010A AT 512453 A5 AT512453 A5 AT 512453A5
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AT
Austria
Prior art keywords
nucleic acid
biosynthetic
program
acid sequence
enzymes
Prior art date
Application number
AT94962010A
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English (en)
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AT512453B1 (de
AT512453A2 (de
Inventor
Tina Lebar
Jernej Turnsek
Tina Ilc
Nejc Tomsic
Roman Jerala
Monika Avbelj
Mojca Bencina
Jerneja Mori
Tjasa Stosicki
Matej Znidaric
Jure Bordon
Mattia Petroni
Rok Gaber
Tomaz Koprivnjak
Gregor Anderluh
Irena Vovk
Vesna Glavnik
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Kemijski Inst
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Publication date
Application filed by Kemijski Inst filed Critical Kemijski Inst
Priority to ATA9496/2010A priority Critical patent/AT512453B1/de
Publication of AT512453A2 publication Critical patent/AT512453A2/de
Publication of AT512453A5 publication Critical patent/AT512453A5/de
Application granted granted Critical
Publication of AT512453B1 publication Critical patent/AT512453B1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1044—Preparation or screening of libraries displayed on scaffold proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Die Erfindung bezieht sich auf eine Methode zur Synthese einer Verbindung mit Hilfe von biosynthetischen Wegen mit der Kultivierung von genetisch veränderten Wirtszellen, wobei die Zellen mit einer oder mehreren Nukleinsäuren verändert werden, wobei die Nukleinsäure die Nukleotidsequenzen für chimäre Proteine beinhalten, die aus Enzymen des biosynthetischen Wegs oder anderen funktionellen Polypeptiden sowie Faktoren, die sich an Nukleinsäure binden, zusammengesetzt sind, und die Programm-Nukleinsäuresequenz, die Zielelemente für Nukleinsäure enthalten, die von den genannten Faktoren des chimären Proteins erkannt werden, wobei sich die Faktoren an die Nukleinsäure binden und die genannte Programm-Nukleotisequenz zur Anordnung der Enzyme des biosynthetischen Wegs oder anderen funktionellen Polypeptiden entlang der Programm-Nukleinsäuresequenz dient und die genannte Kultivierung zur Synthese von genannten chimären Proteinen in genetisch veränderten Wirtszellen dient, was in der Produktion der genannten Verbindung resultiert. Die Erfindung bezieht sich auf Wirtszellen mit der Programm-Nukleinsäuresequenz, mit der Nukleinsäuresequenz, die die chimären Proteine exprimiert. Die Erfindung beschreibt die Methoden zu einer besseren Synthese der Verbindung.
ATA9496/2010A 2010-10-22 2010-10-22 Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv AT512453B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ATA9496/2010A AT512453B1 (de) 2010-10-22 2010-10-22 Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/SI2010/000058 WO2012053985A1 (en) 2010-10-22 2010-10-22 Improved synthesis of biosynthetic product by ordered assembly of biosynthetic enzymes guided by the nucleotide sequence motif template
ATA9496/2010A AT512453B1 (de) 2010-10-22 2010-10-22 Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv

Publications (3)

Publication Number Publication Date
AT512453A2 AT512453A2 (de) 2013-08-15
AT512453A5 true AT512453A5 (de) 2015-04-15
AT512453B1 AT512453B1 (de) 2015-05-15

Family

ID=43769307

Family Applications (1)

Application Number Title Priority Date Filing Date
ATA9496/2010A AT512453B1 (de) 2010-10-22 2010-10-22 Verbesserte Synthese eines biosynthetischen Produkts mit einer angeordneten Zusammensetzung der biosynthetischen Enzyme auf einem Nukleotidsequenzmotiv

Country Status (2)

Country Link
AT (1) AT512453B1 (de)
WO (1) WO2012053985A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9322023B2 (en) * 2011-10-06 2016-04-26 Cornell University Constructs and methods for the assembly of biological pathways
FR3052788A1 (fr) * 2016-06-15 2017-12-22 Univ Claude Bernard Lyon Vecteurs et banques de cellules pour une methode de criblage d'interaction proteine-proteine
WO2025084390A1 (ja) * 2023-10-20 2025-04-24 サントリーホールディングス株式会社 花色の改変を伴う形質転換植物及びこれに含まれる新規化合物

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056904A1 (en) * 1997-06-12 1998-12-17 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818424B2 (en) * 2000-09-01 2004-11-16 E. I. Du Pont De Nemours And Company Production of cyclic terpenoids
DE10063712C1 (de) * 2000-12-20 2002-08-29 Stiftung A Wegener Inst Polar Mikrobiologisches Verfahren zur Biosynthese der natürlichen blau-violetten Farbstoffe Violacein und Desoxyviolacein und deren Verwendung
US7105634B2 (en) * 2002-02-11 2006-09-12 E. I. Du Pont De Nemours And Company Genetic constructs encoding carotenoid biosynthetic enzymes
US7772444B2 (en) * 2005-05-19 2010-08-10 E. I. Du Pont De Nemours And Company Method for the production of resveratrol in a recombinant oleaginous microorganism
US8765403B2 (en) 2008-02-28 2014-07-01 The Regents Of The University Of California Use of synthetic scaffolds for the production of biosynthetic pathway products
JP5632370B2 (ja) * 2008-07-11 2014-11-26 ツィンファ ユニバーシティ デオキシビオラセインを産生する組み換え細菌及びその使用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998056904A1 (en) * 1997-06-12 1998-12-17 Rigel Pharmaceuticals, Inc. Combinatorial enzymatic complexes

Also Published As

Publication number Publication date
AT512453B1 (de) 2015-05-15
WO2012053985A1 (en) 2012-04-26
AT512453A2 (de) 2013-08-15

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