ATA228592A - Verfahren zur trennung von nukleinsäuren - Google Patents

Verfahren zur trennung von nukleinsäuren

Info

Publication number
ATA228592A
ATA228592A AT0228592A AT228592A ATA228592A AT A228592 A ATA228592 A AT A228592A AT 0228592 A AT0228592 A AT 0228592A AT 228592 A AT228592 A AT 228592A AT A228592 A ATA228592 A AT A228592A
Authority
AT
Austria
Prior art keywords
nucleic acids
polymer beads
separating nucleic
alkylated
microns
Prior art date
Application number
AT0228592A
Other languages
English (en)
Other versions
AT398973B (de
Original Assignee
Bonn Guenther Dr
Huber Christian Mag
Oefner Peter Dr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT0228592A priority Critical patent/AT398973B/de
Application filed by Bonn Guenther Dr, Huber Christian Mag, Oefner Peter Dr filed Critical Bonn Guenther Dr
Priority to DK94902238T priority patent/DK0669897T3/da
Priority to AT94902238T priority patent/ATE176454T1/de
Priority to ES94902238T priority patent/ES2130396T3/es
Priority to DE69323435T priority patent/DE69323435T2/de
Priority to US08/153,046 priority patent/US5585236A/en
Priority to EP94902238A priority patent/EP0669897B1/de
Priority to PCT/US1993/010975 priority patent/WO1994011305A1/en
Priority to AU56678/94A priority patent/AU5667894A/en
Priority to JP51239094A priority patent/JP3395906B2/ja
Publication of ATA228592A publication Critical patent/ATA228592A/de
Application granted granted Critical
Publication of AT398973B publication Critical patent/AT398973B/de
Priority to GR990400952T priority patent/GR3029863T3/el

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/803Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Saccharide Compounds (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
AT0228592A 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren AT398973B (de)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren
AT94902238T ATE176454T1 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten
ES94902238T ES2130396T3 (es) 1992-11-18 1993-11-17 Separacion de fragmentos de acido nucleico.
DE69323435T DE69323435T2 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten
DK94902238T DK0669897T3 (da) 1992-11-18 1993-11-17 Adskillelse af nucleinsyrefragmenter
US08/153,046 US5585236A (en) 1992-11-18 1993-11-17 Nucleic acid separation on alkylated nonporous polymer beads
EP94902238A EP0669897B1 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten
PCT/US1993/010975 WO1994011305A1 (en) 1992-11-18 1993-11-17 Separation of nucleic acid fragments
AU56678/94A AU5667894A (en) 1992-11-18 1993-11-17 Separation of nucleic acid fragments
JP51239094A JP3395906B2 (ja) 1992-11-18 1993-11-17 核酸断片の分離法
GR990400952T GR3029863T3 (en) 1992-11-18 1999-04-05 Separation of nucleic acid fragments.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren

Publications (2)

Publication Number Publication Date
ATA228592A true ATA228592A (de) 1994-07-15
AT398973B AT398973B (de) 1995-02-27

Family

ID=3531515

Family Applications (2)

Application Number Title Priority Date Filing Date
AT0228592A AT398973B (de) 1992-11-18 1992-11-18 Verfahren zur trennung von nukleinsäuren
AT94902238T ATE176454T1 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT94902238T ATE176454T1 (de) 1992-11-18 1993-11-17 Trennung von nukleinsäurefragmenten

Country Status (10)

Country Link
US (1) US5585236A (de)
EP (1) EP0669897B1 (de)
JP (1) JP3395906B2 (de)
AT (2) AT398973B (de)
AU (1) AU5667894A (de)
DE (1) DE69323435T2 (de)
DK (1) DK0669897T3 (de)
ES (1) ES2130396T3 (de)
GR (1) GR3029863T3 (de)
WO (1) WO1994011305A1 (de)

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US5795976A (en) * 1995-08-08 1998-08-18 The Board Of Trustees Of The Leland Stanford Junior University Detection of nucleic acid heteroduplex molecules by denaturing high-performance liquid chromatography and methods for comparative sequencing
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography
US6355791B1 (en) 1995-11-13 2002-03-12 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
US6066258A (en) * 1997-12-05 2000-05-23 Transgenomic, Inc. Polynucleotide separations on polymeric separation media
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US5972222A (en) * 1996-11-13 1999-10-26 Transgenomic, Inc. Process for performing polynucleotide separations
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US7138518B1 (en) * 1996-11-13 2006-11-21 Transgenomic, Inc. Liquid chromatographic separation of polynucleotides
US6017457A (en) * 1996-11-13 2000-01-25 Transgenomic, Inc. Method for performing polynucleotide separations using liquid chromatography
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US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
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WO1998048914A1 (en) * 1997-04-25 1998-11-05 Transgenomic, Inc. Improved liquid chromatographic media for polynucleotide separation
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Also Published As

Publication number Publication date
DK0669897T3 (da) 1999-09-20
GR3029863T3 (en) 1999-07-30
ATE176454T1 (de) 1999-02-15
EP0669897B1 (de) 1999-02-03
AU5667894A (en) 1994-06-08
JP3395906B2 (ja) 2003-04-14
ES2130396T3 (es) 1999-07-01
JPH08505700A (ja) 1996-06-18
AT398973B (de) 1995-02-27
US5585236A (en) 1996-12-17
WO1994011305A1 (en) 1994-05-26
DE69323435T2 (de) 1999-07-01
DE69323435D1 (de) 1999-03-18
EP0669897A1 (de) 1995-09-06
EP0669897A4 (de) 1996-01-24

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