ATE104338T1 - Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsaeure und dadurch verbessertes verfahren. - Google Patents

Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsaeure und dadurch verbessertes verfahren.

Info

Publication number: ATE104338T1
Authority: AT; Austria
Prior art keywords: nucleic acid; improved; acid isolation; concentration composition; composition
Prior art date: 1989-05-22

Application number

AT89906232T

Other languages

English (en)

Inventor

David A Debonville

Gerard E Riedel

Original Assignee

Genetics Inst

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-05-22

Filing date

1989-05-22

Publication date

1994-04-15

1989-05-22 Application filed by Genetics Inst filed Critical Genetics Inst

1994-04-15 Application granted granted Critical

1994-04-15 Publication of ATE104338T1 publication Critical patent/ATE104338T1/de

Links

239000000203 mixture Substances 0.000 title abstract 3
150000007523 nucleic acids Chemical class 0.000 title abstract 3
102000039446 nucleic acids Human genes 0.000 title abstract 3
108020004707 nucleic acids Proteins 0.000 title abstract 3
238000000034 method Methods 0.000 title abstract 2
238000002955 isolation Methods 0.000 title 1
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 abstract 2
239000006143 cell culture medium Substances 0.000 abstract 2
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 1
159000000021 acetate salts Chemical class 0.000 abstract 1
239000003153 chemical reaction reagent Substances 0.000 abstract 1
239000012266 salt solution Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/803—Physical recovery methods, e.g. chromatography, grinding

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Life Sciences & Earth Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Crystallography & Structural Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Saccharide Compounds (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

AT89906232T 1989-05-22 1989-05-22 Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsaeure und dadurch verbessertes verfahren. ATE104338T1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
PCT/US1989/002234 WO1990014416A1 (en)	1989-05-22	1989-05-22	Improved composition for isolating and purifying nucleic acid and improved method using same
EP89906232A EP0473575B1 (de)	1989-05-22	1989-05-22	Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsäure und dadurch verbessertes verfahren
CA000600340A CA1328839C (en)	1989-05-22	1989-05-23	Composition for isolating and purifying nucleic acid and improved method using same

Publications (1)

Publication Number	Publication Date
ATE104338T1 true ATE104338T1 (de)	1994-04-15

Family

ID=27423079

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT89906232T ATE104338T1 (de)	1989-05-22	1989-05-22	Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsaeure und dadurch verbessertes verfahren.

Country Status (7)

Country	Link
US (1)	US5047345A (de)
EP (1)	EP0473575B1 (de)
AT (1)	ATE104338T1 (de)
AU (1)	AU635207B2 (de)
CA (1)	CA1328839C (de)
DE (1)	DE68914689T2 (de)
WO (1)	WO1990014416A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5204246A (en) *	1990-12-26	1993-04-20	Pioneer Hi-Bred International, Inc.	Dna isolation method
US5352777A (en) *	1990-12-26	1994-10-04	Pioneer Hi-Bred International, Inc.	DNA isolation from animal cells
IT1252174B (it) *	1991-12-09	1995-06-05	Crinos Industria Farmaco	Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
GB9320459D0 (en) *	1993-10-05	1993-11-24	Nbl Gene Sciences Ltd	In vitro method for single-standed dna preparation
ES2118046B1 (es) *	1996-11-22	1999-04-01	Alava Marquinez Jose Inaki	Procedimiento de insercion de adn en piedras preciosas.
GB2338236B (en) *	1998-06-13	2003-04-09	Aea Technology Plc	Microbiological cell processing
US6750059B1 (en)	1998-07-16	2004-06-15	Whatman, Inc.	Archiving of vectors
WO2002006456A1 (en) *	2000-07-13	2002-01-24	Invitrogen Corporation	Methods and compositions for rapid protein and peptide extraction and isolation using a lysis matrix
KR20020029476A (ko) *	2000-10-13	2002-04-19	박제철	한 단계에 의해 유전자를 추출하기 위한 라이시스버퍼시약
KR20020029477A (ko) *	2000-10-13	2002-04-19	박제철	한 단계에 의한 유전자 추출방법
DE10113815A1 (de) *	2001-03-21	2002-10-02	Eppendorf Ag	Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
EP2216415B2 (de) *	2003-08-01	2017-01-04	Life Technologies Corporation	Zusammensetzungen und Verfahren zur Aufreinigung kurzer RNA-Moleküle und anderer Nukleinsäuren
US7824389B2 (en) *	2003-10-24	2010-11-02	Kimberly-Clark Worldwide, Inc.	Disposable absorbent undergarment for males
KR20060129340A (ko) *	2003-12-30	2006-12-15	시그마-알드리치컴퍼니	효소 소화에 의한 핵산의 신속한 제조
JP4810164B2 (ja) *	2004-09-03	2011-11-09	富士フイルム株式会社	核酸分離精製方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4843012A (en) *	1986-09-17	1989-06-27	Genetics Institute, Inc.	Novel composition for nucleic acid purification
US4833239A (en) *	1987-01-29	1989-05-23	Genetics Institute, Inc.	Method for the isolation and purification of DNA molecules

1989
- 1989-05-22 DE DE68914689T patent/DE68914689T2/de not_active Expired - Fee Related
- 1989-05-22 EP EP89906232A patent/EP0473575B1/de not_active Expired - Lifetime
- 1989-05-22 AU AU36961/89A patent/AU635207B2/en not_active Ceased
- 1989-05-22 AT AT89906232T patent/ATE104338T1/de not_active IP Right Cessation
- 1989-05-22 WO PCT/US1989/002234 patent/WO1990014416A1/en not_active Ceased
- 1989-05-23 CA CA000600340A patent/CA1328839C/en not_active Expired - Fee Related
- 1989-06-02 US US07/360,276 patent/US5047345A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
AU635207B2 (en)	1993-03-18
DE68914689T2 (de)	1994-10-27
EP0473575A1 (de)	1992-03-11
CA1328839C (en)	1994-04-26
EP0473575B1 (de)	1994-04-13
WO1990014416A1 (en)	1990-11-29
EP0473575A4 (en)	1992-08-19
US5047345A (en)	1991-09-10
AU3696189A (en)	1990-12-18
DE68914689D1 (de)	1994-05-19

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Legal Events

Date	Code	Title	Description
1994-10-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties