ATE109835T1 - Durch die verwendung von substraten, deren fluoreszenzeigenschaften sich von denen der umgewandelten produkte unterscheiden, gekennzeichnete methode zur bestimmung der enzymaktivität. - Google Patents

Durch die verwendung von substraten, deren fluoreszenzeigenschaften sich von denen der umgewandelten produkte unterscheiden, gekennzeichnete methode zur bestimmung der enzymaktivität.

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Publication number
ATE109835T1
ATE109835T1 AT89905803T AT89905803T ATE109835T1 AT E109835 T1 ATE109835 T1 AT E109835T1 AT 89905803 T AT89905803 T AT 89905803T AT 89905803 T AT89905803 T AT 89905803T AT E109835 T1 ATE109835 T1 AT E109835T1
Authority
AT
Austria
Prior art keywords
enzyme
pct
fluorescent properties
chromophore
substrates
Prior art date
Application number
AT89905803T
Other languages
English (en)
Inventor
Hakan Drevin
Anna-Tora Martin
Jan Carlsson
Sven Olov Oscarsson
Timo Loevgren
Ilkka Hemmilae
Marek Kwiatkowski
Original Assignee
Kabi Pharmacia Ab
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=20372243&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE109835(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Kabi Pharmacia Ab, Wallac Oy filed Critical Kabi Pharmacia Ab
Application granted granted Critical
Publication of ATE109835T1 publication Critical patent/ATE109835T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT89905803T 1988-05-05 1989-04-28 Durch die verwendung von substraten, deren fluoreszenzeigenschaften sich von denen der umgewandelten produkte unterscheiden, gekennzeichnete methode zur bestimmung der enzymaktivität. ATE109835T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8801702A SE8801702D0 (sv) 1988-05-05 1988-05-05 Sett vid bestemning av enzymatisk aktivitet

Publications (1)

Publication Number Publication Date
ATE109835T1 true ATE109835T1 (de) 1994-08-15

Family

ID=20372243

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89905803T ATE109835T1 (de) 1988-05-05 1989-04-28 Durch die verwendung von substraten, deren fluoreszenzeigenschaften sich von denen der umgewandelten produkte unterscheiden, gekennzeichnete methode zur bestimmung der enzymaktivität.

Country Status (9)

Country Link
US (1) US5252462A (de)
EP (1) EP0374221B1 (de)
JP (1) JPH02504109A (de)
AT (1) ATE109835T1 (de)
AU (1) AU622904B2 (de)
CA (1) CA1338167C (de)
DE (1) DE68917438T2 (de)
SE (1) SE8801702D0 (de)
WO (1) WO1989010975A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI901680A7 (fi) * 1989-04-10 1990-10-11 Univ Of Georgia Research Entsyymiluminesenssianalyysi
GB8927503D0 (en) * 1989-12-04 1990-02-07 Kronem Systems Inc Enzyme-amplified lanthanide chelate luminescence
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
CA2371816C (en) * 1999-02-18 2010-04-27 The Regents Of The University Of California Phthalamide-lanthanide complexes for use as luminescent markers
US6329205B1 (en) 1999-08-31 2001-12-11 Molecular Probes, Inc. Detection method using luminescent europium-based protein stains
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
FR2824141B1 (fr) * 2001-04-25 2005-12-23 Proteus Methode de detection de la transformation d'un substrat par mesure d'un changement de pm, les produits mis en oeuvre et detectes selon ladite methode
ATE407176T1 (de) * 2003-12-30 2008-09-15 Univ California Office Of The Aromatische triamid-lanthanid-komplexe
WO2006039505A2 (en) * 2004-09-30 2006-04-13 Molecular Devices Corporation Luminescent lanthanide complexes
US8557601B2 (en) 2006-07-10 2013-10-15 The Regents Of The University Of California Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
US8173800B2 (en) * 2006-08-15 2012-05-08 The Regents Of The University Of California Luminescent macrocyclic lanthanide complexes
US8507199B2 (en) * 2007-01-25 2013-08-13 Lumiphore, Inc. Multi-color time resolved fluorophores based on macrocyclic lanthanide complexes
US8889839B2 (en) * 2008-05-07 2014-11-18 Syracuse University Pyridine-bis (oxazoline)(“pybox”) moiety as a chelator and sensitizer for lanthanide ion (Ln (III)) Luminescence
WO2010051544A2 (en) * 2008-10-31 2010-05-06 Lumiphore, Inc. Rapid homogeneous diagnostic testing platform based on lanthanide fluorescent resonance energy transfer
WO2011025790A1 (en) 2009-08-24 2011-03-03 Lumiphore, Inc. Macrocyclic hopo chelators
JP2013515744A (ja) * 2009-12-24 2013-05-09 ルミフォア，インコーポレイテッド 放射性医薬品錯体
JP5818199B2 (ja) * 2011-05-24 2015-11-18 国立大学法人 東京大学 蛍光を利用したニトリル関連酵素の酵素活性検出方法
EP2825309B1 (de) 2012-03-16 2018-05-16 Stat-Diagnostica & Innovation, S.L. Messkartousche mit trasportmodul
US11453652B2 (en) 2013-03-15 2022-09-27 Lumiphore, Inc. Di-macrocycles
CN114736255B (zh) * 2022-05-11 2023-10-27 湖南超亟检测技术有限责任公司 检测β-半乳糖苷酶的黄酮衍生物荧光探针及其制备方法和应用、试剂盒及其使用方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4837169A (en) * 1981-07-01 1989-06-06 Eastman Kodak Company Polypyridine Fluorescent labels for immunoassay
CA1205028A (en) * 1981-07-01 1986-05-27 Jerald C. Hinshaw Fluorescent chelates and labeled specific binding reagents prepared therefrom
SE454115B (sv) * 1982-09-13 1988-03-28 Wallac Oy Homogenfasanalys med lantanidkelat som merksubstans
GB8421318D0 (en) * 1984-08-22 1984-09-26 Ekins R P Potentiating antibodies/other macro-molecules
ATE78517T1 (de) * 1985-02-25 1992-08-15 Abbott Lab Polarisierter fluoreszenztest von makromolekularen hydrolasen.
SE8703682L (sv) * 1987-09-24 1989-03-25 Wallac Oy Homogen bestaemningsmetod som utnyttjar affinitetsreaktioner
DE3732871A1 (de) * 1987-09-30 1989-04-20 Behringwerke Ag Chromogene substrate
US5011910A (en) * 1989-12-28 1991-04-30 Washington University Reagent and method for determining activity of retroviral protease

Also Published As

Publication number Publication date
CA1338167C (en) 1996-03-19
SE8801702D0 (sv) 1988-05-05
EP0374221A1 (de) 1990-06-27
US5252462A (en) 1993-10-12
EP0374221B1 (de) 1994-08-10
WO1989010975A1 (en) 1989-11-16
DE68917438D1 (de) 1994-09-15
DE68917438T2 (de) 1995-05-24
AU622904B2 (en) 1992-04-30
JPH02504109A (ja) 1990-11-29
AU3567889A (en) 1989-11-29

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