ATE115727T1 - Silberfärbungstechnik und kit dafür. - Google Patents

Silberfärbungstechnik und kit dafür.

Info

Publication number: ATE115727T1
Authority: AT; Austria
Prior art keywords: support medium; kit; silver staining; technique; sensitizer
Prior art date: 1988-09-16

Application number

AT89117124T

Other languages

English (en)

Inventor

Nobuyoshi Ebata

Yuko Tanaka

Akiko Negishi

Original Assignee

Daiichi Pure Chemicals Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-09-16

Filing date

1989-09-15

Publication date

1994-12-15

1988-09-16 Priority claimed from JP63232058A external-priority patent/JPH0280958A/ja

1988-09-16 Priority claimed from JP63232059A external-priority patent/JP2610493B2/ja

1989-09-15 Application filed by Daiichi Pure Chemicals Co Ltd filed Critical Daiichi Pure Chemicals Co Ltd

1994-12-15 Application granted granted Critical

1994-12-15 Publication of ATE115727T1 publication Critical patent/ATE115727T1/de

Links

238000000034 method Methods 0.000 title abstract 3
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title 1
229910052709 silver Inorganic materials 0.000 title 1
239000004332 silver Substances 0.000 title 1
208000002109 Argyria Diseases 0.000 abstract 3
239000003638 chemical reducing agent Substances 0.000 abstract 2
239000003795 chemical substances by application Substances 0.000 abstract 2
UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 abstract 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 1
238000001514 detection method Methods 0.000 abstract 1
239000012192 staining solution Substances 0.000 abstract 1
DHCDFWKWKRSZHF-UHFFFAOYSA-N sulfurothioic S-acid Chemical compound OS(O)(=O)=S DHCDFWKWKRSZHF-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/683—Total protein determination, e.g. albumin in urine involving metal ions
- G01N33/6836—Silver staining
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/305—Fixative compositions
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/905—Photochemical activation of reactions
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Urology & Nephrology (AREA)
Physics & Mathematics (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Pathology (AREA)
Biochemistry (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Hematology (AREA)
General Physics & Mathematics (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Biotechnology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biophysics (AREA)
Bioinformatics & Computational Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Investigating Or Analysing Biological Materials (AREA)

AT89117124T 1988-09-16 1989-09-15 Silberfärbungstechnik und kit dafür. ATE115727T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
JP63232058A JPH0280958A (ja)	1988-09-16	1988-09-16	銀染色法及び銀染色剤キツト
JP63232059A JP2610493B2 (ja)	1988-09-16	1988-09-16	銀染色方法及び銀染色剤キツト

Publications (1)

Publication Number	Publication Date
ATE115727T1 true ATE115727T1 (de)	1994-12-15

Family

ID=26530263

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT89117124T ATE115727T1 (de)	1988-09-16	1989-09-15	Silberfärbungstechnik und kit dafür.

Country Status (4)

Country	Link
US (1)	US5064768A (de)
EP (1)	EP0359281B1 (de)
AT (1)	ATE115727T1 (de)
DE (1)	DE68919955T2 (de)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA2071736A1 (en) *	1991-06-24	1992-12-25	Naoyuki Kouno	Acid dye staining method
US5795785A (en) *	1992-12-10	1998-08-18	Suslov; Eugene Ivanovich	Method for the diagnosis of human malignant tumors
US5595878A (en) *	1995-06-02	1997-01-21	Boron Biologicals, Inc.	Detection of biopolymers and biooligomers with boron hydride labels
US6068754A (en) *	1998-04-27	2000-05-30	Koh; Francis H.	Multi-tank gel developing apparatus and method
AU2002230438A1 (en) *	2000-11-20	2002-06-03	Invitrogen Corporation	Materials for enhancing staining of biopolymers in matrices
AU2002223344A1 (en) *	2000-11-28	2002-06-11	Mcmaster University	Sputum fixative and methods and uses therefor
CA2442282A1 (en) *	2001-03-29	2002-10-10	Cellect Technologies Corp.	Methods devices and systems for sorting and separating particles
US7662640B2 (en) *	2004-09-28	2010-02-16	Kyoto University	Method for silver staining of biological substances
CN102081018A (zh) *	2009-11-30	2011-06-01	希森美康株式会社	样品的预处理方法及hcv的免疫测定方法
WO2018209178A1 (en)	2017-05-11	2018-11-15	The Research Foundation For The State University Of New York	Method and kit for determining the presence of monosodium urate crystals in joint synovial fluid

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4416998A (en) *	1981-04-02	1983-11-22	The Upjohn Company	Silver stain procedure and kit for same
FI821071A7 (fi) *	1981-04-02	1982-10-03	Health Products Inc	Hopeaa sitovan aineen värjäysmenetelmä ja -välinesarja.
US4468466A (en) *	1982-02-16	1984-08-28	The United States Of America As Represented By The Department Of Health And Human Services	Silver stains for protein in gels--a modified procedure
JPS59217164A (ja) *	1983-05-26	1984-12-07	Dai Ichi Pure Chem Co Ltd	銀染色方法
US4690901A (en) *	1984-01-26	1987-09-01	The University Of North Carolina At Chapel Hill	Silver methenamine staining method
US4575452A (en) *	1984-09-21	1986-03-11	E. I. Du Pont De Nemours And Company	Kit for silver staining proteins and nucleic acids
CA1225317A (en) *	1984-12-04	1987-08-11	Gerald Krystal	Protein assay by silver binding
US4703016A (en) *	1986-05-05	1987-10-27	The United States Of America As Represented By The Department Of Health And Human Services	Silver stain for rapid, quantitative detection of polypeptides and nucleic acids

1989
- 1989-09-12 US US07/405,479 patent/US5064768A/en not_active Expired - Lifetime
- 1989-09-15 AT AT89117124T patent/ATE115727T1/de not_active IP Right Cessation
- 1989-09-15 EP EP89117124A patent/EP0359281B1/de not_active Expired - Lifetime
- 1989-09-15 DE DE68919955T patent/DE68919955T2/de not_active Expired - Fee Related

Also Published As

Publication number	Publication date
EP0359281B1 (de)	1994-12-14
EP0359281A2 (de)	1990-03-21
DE68919955D1 (de)	1995-01-26
DE68919955T2 (de)	1995-07-06
EP0359281A3 (de)	1991-01-16
US5064768A (en)	1991-11-12

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Legal Events

Date	Code	Title	Description
1995-05-15	UEP	Publication of translation of european patent specification
2006-06-15	REN	Ceased due to non-payment of the annual fee