ATE141649T1 - Verfahren zum nachweis von hydrolytischen enzymen - Google Patents

Verfahren zum nachweis von hydrolytischen enzymen

Info

Publication number
ATE141649T1
ATE141649T1 AT92305109T AT92305109T ATE141649T1 AT E141649 T1 ATE141649 T1 AT E141649T1 AT 92305109 T AT92305109 T AT 92305109T AT 92305109 T AT92305109 T AT 92305109T AT E141649 T1 ATE141649 T1 AT E141649T1
Authority
AT
Austria
Prior art keywords
resin
binding
assay method
scissile bond
plates
Prior art date
Application number
AT92305109T
Other languages
English (en)
Inventor
William Francis Heath Jr
Mei-Huei Tsai Lai
Joseph Vincent Manetta
John Richard Sportsman
Sau-Chi Betty Yan
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Application granted granted Critical
Publication of ATE141649T1 publication Critical patent/ATE141649T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K7/00Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04Linear peptides containing only normal peptide links
    • C07K7/22Tachykinins, e.g. Eledoisins, Substance P; Related peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00Reverse transcribing RNA viruses
    • C12N2740/00011Details
    • C12N2740/10011Retroviridae
    • C12N2740/16011Human Immunodeficiency Virus, HIV
    • C12N2740/16211Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/15Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16HIV-1, HIV-2
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT92305109T 1991-06-10 1992-06-03 Verfahren zum nachweis von hydrolytischen enzymen ATE141649T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/712,828 US5235039A (en) 1991-06-10 1991-06-10 Substrates for hiv protease

Publications (1)

Publication Number Publication Date
ATE141649T1 true ATE141649T1 (de) 1996-09-15

Family

ID=24863712

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92305109T ATE141649T1 (de) 1991-06-10 1992-06-03 Verfahren zum nachweis von hydrolytischen enzymen

Country Status (7)

Country Link
US (1) US5235039A (de)
EP (1) EP0518557B1 (de)
JP (1) JPH05194591A (de)
AT (1) ATE141649T1 (de)
CA (1) CA2070719A1 (de)
DE (1) DE69212911D1 (de)
DK (1) DK0518557T3 (de)

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US6071895A (en) * 1992-03-11 2000-06-06 Narhex Limited Polar-substituted hydrocarbons
JPH07504654A (ja) * 1992-03-11 1995-05-25 ナルヘックス リミテッド オキソ及びヒドロキシ置換炭化水素のアミン誘導体
MXPA93002392A (es) 1992-03-11 2005-02-04 Narhex Ltd Derivados amino de hidrocarburos-oxo e hidroxi-substituidos.
US5888992A (en) * 1992-03-11 1999-03-30 Narhex Limited Polar substituted hydrocarbons
DE4217474A1 (de) * 1992-05-27 1993-12-02 Boehringer Mannheim Gmbh Verfahren und Mittel zur Bestimmung eines Analyts
US5484926A (en) * 1993-10-07 1996-01-16 Agouron Pharmaceuticals, Inc. HIV protease inhibitors
US5846993A (en) * 1992-12-22 1998-12-08 Agouron Pharmaceuticals, Inc. HIV protease inhibitors
US6348310B1 (en) * 1994-03-04 2002-02-19 Promega Corporation Quantitation of individual protein kinase activity
ATE165168T1 (de) * 1994-03-29 1998-05-15 Lepetit Spa Festphasenimmunoassay zum nachweis von inhibitoren von einem proteolytischen enzym
US5527829A (en) * 1994-05-23 1996-06-18 Agouron Pharmaceuticals, Inc. HIV protease inhibitors
ATE296877T1 (de) * 1994-10-07 2005-06-15 Univ Rockefeller Enzyme zur spaltung der verankerungsregion von oberflächenproteinen aus gram-positiven bakterien
ATE220661T1 (de) * 1994-10-19 2002-08-15 Novartis Erfind Verwalt Gmbh Antivirale ether von aspartat-protease-substrat- isosteren
WO1997025437A1 (en) * 1996-01-04 1997-07-17 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Method for assaying proteolytic enzymes using fluorescence-quenched substrates
US6333303B1 (en) 1996-04-30 2001-12-25 Twinstrand Therapeutics Inc. Antiviral ricin-like proteins
US5925759A (en) 1996-09-05 1999-07-20 Agouron Pharmaceuticals, Inc. Methods of making HIV-protease inhibitors and intermediates for making HIV-protease inhibitors
US5705647A (en) * 1996-09-05 1998-01-06 Agouron Pharmaceuticals, Inc. Intermediates for making HIV-protease inhibitors
US5962725A (en) 1996-09-05 1999-10-05 Agouron Pharmaceuticals, Inc. Intermediate compounds useful for making HIV protease inhibitors such as nelfinavir
US6001851A (en) * 1997-03-13 1999-12-14 Agouron Pharmaceuticals, Inc. HIV protease inhibitors
AU4355097A (en) * 1997-05-01 1998-11-27 Minnesota Mining And Manufacturing Company Fluorogenic protease substrates based on dye-dimerization
US6084107A (en) * 1997-09-05 2000-07-04 Agouron Pharmaceuticals, Inc. Intermediates for making HIV-protease inhibitors
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US6908770B1 (en) 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US6312896B1 (en) * 1998-09-17 2001-11-06 Igen Inaternational, Inc. Assays for measuring nucleic acid binding proteins and enzyme activities
US6911307B1 (en) 1998-12-08 2005-06-28 Proteus S.A. Method of detection in vitro of a target substance in a sample comprising the labelling of said substance with a reporter gene and with the sequences necessary for the expression of said reporter gene in vitro
FR2786787B1 (fr) * 1998-12-08 2002-04-19 Proteus Methode d'analyse in vitro d'un phenotype connu a partir d'un echantillon d'acides nucleiques
FR2786789B1 (fr) * 1998-12-08 2001-12-28 Proteus Methode de detection in vitro d'une sequence d'acide nucleique cible dans un echantillon d'acide nucleique
FR2786788B1 (fr) * 1998-12-08 2002-04-19 Proteus Procede de criblage de substances capables de modifier l'activite d'une ou plusieurs proteines cibles ou d'un ensemble cible de proteines exprimees in vitro
US6531125B1 (en) 1999-03-02 2003-03-11 Twinstrand Therapeutics Inc. Antiviral ricin-like proteins
CA2270639A1 (en) * 1999-05-10 2000-11-10 Zhibo Gan A method for measuring biomolecules
MXPA01012270A (es) 1999-05-28 2003-06-24 Virco Nv Nuevos perfiles mutacionales en la transcriptasa reversa de vih-1 correlacionadas con resistencia fenotipica a farmacos.
AU5604900A (en) * 1999-06-09 2000-12-28 Ljl Biosystems, Inc. Phosphorylation assays
US7022517B1 (en) 1999-07-16 2006-04-04 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
CA2379130A1 (en) 1999-07-16 2001-01-25 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
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EP1356082A2 (de) 2000-10-20 2003-10-29 Virco Bvba Mutationsprofile bei mit phänotypischer medikamentenresistenz korrelierter hiv-1-reverse-transkriptase
US6818396B1 (en) 2000-11-28 2004-11-16 Proteus S.A. Process for determination of the activity of a substance using an in vitro functional test
WO2003023360A2 (en) * 2001-09-10 2003-03-20 Meso Scale Technologies, Llc Methods and apparatus for conducting multiple measurements on a sample
WO2003040390A2 (en) 2001-11-08 2003-05-15 Tibotec Pharmaceuticals Ltd. Protease assay for therapeutic drug monitoring
AU2003231731A1 (en) * 2002-04-15 2003-11-03 American National Red Cross Method for detecting ligands and targets in a mixture
US20070015230A1 (en) * 2002-04-15 2007-01-18 Hammond David J Identification and characterization of analytes from whole blood
US8257967B2 (en) 2002-04-26 2012-09-04 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
AU2003251732B2 (en) 2002-07-01 2008-09-18 Tibotec Pharmaceuticals Ltd. New mutational profiles in HIV-1 reverse transcriptase correlated with phenotypic drug resistance
US7217506B2 (en) 2002-07-01 2007-05-15 Tibotec Pharmaceuticals, Ltd. Mutational profiles in HIV-1 protease correlated with phenotypic protease inhibitor resistance
CA2549190A1 (en) 2003-12-11 2005-06-30 Board Of Regents, The University Of Texas System Method and system for the analysis of saliva using a sensor array
US7781226B2 (en) 2004-02-27 2010-08-24 The Board Of Regents Of The University Of Texas System Particle on membrane assay system
US8101431B2 (en) 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
US8105849B2 (en) 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
WO2007053186A2 (en) 2005-05-31 2007-05-10 Labnow, Inc. Methods and compositions related to determination and use of white blood cell counts
CN105136699B (zh) * 2015-08-25 2018-03-16 四川大学 基于未变性染色皮粉底物的蛋白酶胶原蛋白水解活力测定方法及其应用

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AU563827B2 (en) * 1982-07-01 1987-07-23 Millipore Corp. Filtration apparatus
US4569907A (en) * 1984-01-16 1986-02-11 Monsanto Company Thiopeptolide substrates for vertebrate collagenase
DE3531778A1 (de) * 1985-09-06 1987-03-12 Behringwerke Ag Verfahren zur bestimmung von proteaseinhibitoren
CA1333883C (en) * 1988-04-07 1995-01-10 Shashidhara H. M. Murthy Immunoassay utilizing biotin bridge with universal solid phase
DE3842197A1 (de) * 1988-12-15 1990-06-21 Hoechst Ag Rasch spaltbares substrat fuer die hiv-protease

Also Published As

Publication number Publication date
US5235039A (en) 1993-08-10
JPH05194591A (ja) 1993-08-03
EP0518557A2 (de) 1992-12-16
EP0518557A3 (en) 1993-06-16
CA2070719A1 (en) 1992-12-11
DK0518557T3 (da) 1996-09-09
EP0518557B1 (de) 1996-08-21
DE69212911D1 (de) 1996-09-26

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