ATE149512T1 - Verfahren und verbindungen zum markieren von dns mit xanthin und niederalkyl substituierten xathinderivaten und reagentrien für die ''in situ'' detektion von chromosomen - Google Patents
Verfahren und verbindungen zum markieren von dns mit xanthin und niederalkyl substituierten xathinderivaten und reagentrien für die ''in situ'' detektion von chromosomenInfo
- Publication number
- ATE149512T1 ATE149512T1 AT91310203T AT91310203T ATE149512T1 AT E149512 T1 ATE149512 T1 AT E149512T1 AT 91310203 T AT91310203 T AT 91310203T AT 91310203 T AT91310203 T AT 91310203T AT E149512 T1 ATE149512 T1 AT E149512T1
- Authority
- AT
- Austria
- Prior art keywords
- xanthine
- chromosome
- lower alkyl
- alkyl substituted
- reagents
- Prior art date
Links
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 title abstract 14
- 210000000349 chromosome Anatomy 0.000 title abstract 9
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 6
- 229940075420 xanthine Drugs 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 150000001875 compounds Chemical class 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 238000002372 labelling Methods 0.000 title 1
- 125000000217 alkyl group Chemical group 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/611,305 US5512433A (en) | 1990-11-08 | 1990-11-08 | Methods and compounds for labeling DNA with xanthine and lower alkyl substituted xanthine derivatives and reagents for the in situ detection of chromosomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE149512T1 true ATE149512T1 (de) | 1997-03-15 |
Family
ID=24448502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91310203T ATE149512T1 (de) | 1990-11-08 | 1991-11-05 | Verfahren und verbindungen zum markieren von dns mit xanthin und niederalkyl substituierten xathinderivaten und reagentrien für die ''in situ'' detektion von chromosomen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US5512433A (de) |
| EP (1) | EP0485156B1 (de) |
| JP (1) | JPH04287699A (de) |
| AT (1) | ATE149512T1 (de) |
| DE (1) | DE69124921T2 (de) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994009022A1 (en) * | 1992-10-09 | 1994-04-28 | Oncor, Inc. | Methods for the detection of chromosome structural abnormalities by in situ hybridization to fixed tissue |
| EP0878552A1 (de) * | 1997-05-13 | 1998-11-18 | Erasmus Universiteit Rotterdam | Molekularer Nachweis von chromosomalen Veränderungen |
| JP2002513587A (ja) * | 1998-05-04 | 2002-05-14 | ダコ エー エス | 染色体異常を検出するための方法およびプローブ |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4213893A (en) * | 1978-10-12 | 1980-07-22 | Miles Laboratories, Inc. | Flavin adenine dinucleotide-labeled conjugates for use in specific binding assays |
| US4533493A (en) * | 1981-08-27 | 1985-08-06 | Miles Laboratories, Inc. | Theophylline immunogens, antibodies, labeled conjugates, and related derivatives |
| US4687737A (en) * | 1982-11-12 | 1987-08-18 | Massachusetts Institute Of Technology | Mammalian suppressor genes |
| US4769377A (en) * | 1983-02-18 | 1988-09-06 | The Johns Hopkins University | Adenosine receptor antagonists |
| US4626501A (en) * | 1983-09-02 | 1986-12-02 | Integrated Genetics, Inc. | Labeled DNA |
| US4699876A (en) * | 1984-01-27 | 1987-10-13 | E. I. Du Pont De Nemours And Company | Nonradiometric polynucleotide probes |
| US4828979A (en) * | 1984-11-08 | 1989-05-09 | Life Technologies, Inc. | Nucleotide analogs for nucleic acid labeling and detection |
| US4833251A (en) * | 1985-06-25 | 1989-05-23 | Siska Diagnostics, Inc. | Compounds for tagging nucleic acid probes |
| EP0310361A3 (de) * | 1987-09-30 | 1989-05-24 | Beckman Instruments, Inc. | Aus drei Verzahnungen bestehendes Konjugat und Verfahren zu seiner Verwendung |
| ES2018959A6 (es) * | 1988-11-15 | 1991-05-16 | Univ Yale | Metodo de delineacion de cromosomas humanos individuales en celulas metafasicas e interfasicas mediante hibridacion de suspension in situ. |
| IL94906A (en) * | 1989-07-19 | 1995-10-31 | Univ California | Methods and preparations for specific chromosome specification |
| US5258507A (en) * | 1990-11-08 | 1993-11-02 | Amoco Corporation | Labeling reagents useful for the chemical attachment of nitrophenyl derivative ligands to DNA probes |
-
1990
- 1990-11-08 US US07/611,305 patent/US5512433A/en not_active Expired - Fee Related
-
1991
- 1991-11-05 EP EP91310203A patent/EP0485156B1/de not_active Expired - Lifetime
- 1991-11-05 AT AT91310203T patent/ATE149512T1/de not_active IP Right Cessation
- 1991-11-05 DE DE69124921T patent/DE69124921T2/de not_active Expired - Fee Related
- 1991-11-08 JP JP3293252A patent/JPH04287699A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE69124921T2 (de) | 1997-10-16 |
| JPH04287699A (ja) | 1992-10-13 |
| EP0485156B1 (de) | 1997-03-05 |
| EP0485156A1 (de) | 1992-05-13 |
| DE69124921D1 (de) | 1997-04-10 |
| US5512433A (en) | 1996-04-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |