ATE151112T1 - Bei hoher temperatur aktive reverse transkriptasen - Google Patents
Bei hoher temperatur aktive reverse transkriptasenInfo
- Publication number
- ATE151112T1 ATE151112T1 AT91904087T AT91904087T ATE151112T1 AT E151112 T1 ATE151112 T1 AT E151112T1 AT 91904087 T AT91904087 T AT 91904087T AT 91904087 T AT91904087 T AT 91904087T AT E151112 T1 ATE151112 T1 AT E151112T1
- Authority
- AT
- Austria
- Prior art keywords
- active
- high temperature
- reverse transcriptases
- reverse transcription
- rna
- Prior art date
Links
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 title 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 title 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000010839 reverse transcription Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 238000012408 PCR amplification Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45596789A | 1989-12-22 | 1989-12-22 | |
| US07/455,611 US5322770A (en) | 1989-12-22 | 1989-12-22 | Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription |
| US58547190A | 1990-09-20 | 1990-09-20 | |
| PCT/US1990/007641 WO1991009944A2 (en) | 1989-12-22 | 1990-12-21 | High temperature reverse transcriptases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE151112T1 true ATE151112T1 (de) | 1997-04-15 |
Family
ID=27412639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91904087T ATE151112T1 (de) | 1989-12-22 | 1990-12-21 | Bei hoher temperatur aktive reverse transkriptasen |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0506889B1 (de) |
| JP (1) | JP2968585B2 (de) |
| AT (1) | ATE151112T1 (de) |
| AU (1) | AU656315B2 (de) |
| CA (1) | CA2071213C (de) |
| DE (1) | DE69030386T2 (de) |
| DK (1) | DK0506889T3 (de) |
| ES (1) | ES2100945T3 (de) |
| GR (1) | GR3023862T3 (de) |
| WO (1) | WO1991009944A2 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5466591A (en) * | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5693517A (en) * | 1987-06-17 | 1997-12-02 | Roche Molecular Systems, Inc. | Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions |
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5795762A (en) * | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5561058A (en) * | 1986-08-22 | 1996-10-01 | Hoffmann-La Roche Inc. | Methods for coupled high temperatures reverse transcription and polymerase chain reactions |
| JP2709311B2 (ja) * | 1990-09-28 | 1998-02-04 | エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト | 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異 |
| AU8906091A (en) * | 1990-10-05 | 1992-04-28 | Wayne M. Barnes | Thermostable dna polymerase |
| CA2038839A1 (en) * | 1991-03-22 | 1992-09-23 | Jiang J. Liu | Method for detection of viral rna |
| AU662906B2 (en) * | 1991-06-26 | 1995-09-21 | F. Hoffmann-La Roche Ag | Methods for detection of carcinoma metastases by nucleic acid amplification |
| ES2111589T3 (es) * | 1991-08-27 | 1998-03-16 | Hoffmann La Roche | Metodos y reactivos para la deteccion de la hepatitis c. |
| USRE39031E1 (en) * | 1992-04-29 | 2006-03-21 | Biomerieux | RNA amplification method requiring only one manipulation step |
| FR2690691B1 (fr) * | 1992-04-29 | 1999-02-12 | Bio Merieux | Procede d'amplification d'arn necessitant une seule etape de manipulation. |
| ES2161731T3 (es) * | 1993-07-01 | 2001-12-16 | Hoffmann La Roche | Reactivos y metodos para la transcripcion inversa acoplada a alta temperatura y reaccion en cadena de la polimerasa. |
| US5474920A (en) * | 1993-11-23 | 1995-12-12 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University | Modified thermo-resistant DNA polymerases |
| DE69431240T2 (de) | 1993-12-01 | 2003-04-17 | Toyo Boseki K.K., Osaka | Verfahren zur ampflifizierung und zum nachweis bestimmter nukleinsäuresequenzen mittels thermostabiler enzyme |
| US5648211A (en) * | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| EP0736609A3 (de) * | 1995-04-08 | 1998-07-29 | Roche Diagnostics GmbH | Verfahren zur spezifischen Vervielfältigung und zum Nachweis von DNA bzw. RNA |
| US5885813A (en) * | 1995-05-31 | 1999-03-23 | Amersham Life Science, Inc. | Thermostable DNA polymerases |
| US6077664A (en) * | 1995-06-07 | 2000-06-20 | Promega Corporation | Thermophilic DNA polymerases from Thermotoga neapolitana |
| US6001645A (en) * | 1995-06-07 | 1999-12-14 | Promega Corporation | Thermophilic DNA polymerases from thermotoga neapolitana |
| EP0866868B1 (de) * | 1995-12-15 | 2001-07-11 | Amersham Life Science Inc | Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität |
| CA2199213C (en) * | 1996-03-11 | 2007-04-17 | John Wesley Backus | Amplifying and detecting target nucleic acids using a post amplification incubation step |
| US6013488A (en) | 1996-07-25 | 2000-01-11 | The Institute Of Physical And Chemical Research | Method for reverse transcription |
| ES2121698B1 (es) * | 1996-12-02 | 1999-07-01 | Inia | Procedimiento para la obtencionn de fragmentos amplificados de acido nucleico de particulas virales y subvirales que contienen rna. |
| US6030814A (en) * | 1997-04-21 | 2000-02-29 | Epicentre Technologies Corporation | Reverse transcription method |
| JP2001515362A (ja) * | 1997-05-13 | 2001-09-18 | ディスプレイ・システムズ・バイオテック・アクティーゼルスカブ | ｍＲＮＡをクローニングする方法および差次的に発現された転写物のディスプレイ（ＤＯＤＥＴ） |
| EP0921196A1 (de) * | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modifizierte DNA Polymerase aus Carboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-kettenreaktion |
| EP0922765B1 (de) * | 1997-12-02 | 2005-11-23 | Roche Diagnostics GmbH | Modifizierte DNA Polymerase aus Corboxydothermus hydrogenoformans und deren Verwendung für gekoppelte reverse Transkription und Polymerase-Kettenreaktion |
| EP1064296B1 (de) * | 1998-03-18 | 2005-10-19 | Amersham Biosciences Corp. | Temperaturstabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus |
| AT410218B (de) | 1999-08-20 | 2003-03-25 | Baxter Ag | Verfahren zur herstellung eines qualitätsgesicherten pools biologischer proben |
| US6632645B1 (en) | 2000-03-02 | 2003-10-14 | Promega Corporation | Thermophilic DNA polymerases from Thermoactinomyces vulgaris |
| US6436677B1 (en) | 2000-03-02 | 2002-08-20 | Promega Corporation | Method of reverse transcription |
| KR20000072141A (ko) * | 2000-08-04 | 2000-12-05 | 김유삼 | 신규한 호열성 단백질 분해효소의 ｄｎａ 서열 및그로부터 유래된 단백질 |
| GB0205455D0 (en) | 2002-03-07 | 2002-04-24 | Molecular Sensing Plc | Nucleic acid probes, their synthesis and use |
| US7575864B2 (en) * | 2004-05-27 | 2009-08-18 | E.I. Du Pont De Nemours And Company | Method for the direct detection of diagnostic RNA |
| US7504240B2 (en) * | 2005-03-10 | 2009-03-17 | Masanori Hirano | Methods for synthesizing polynucleotides using a single primer |
| JP5191041B2 (ja) | 2007-04-05 | 2013-04-24 | エフ．ホフマン−ラ ロシュ アーゲー | 急速ワンステップｒｔ−ｐｃｒ |
| KR101039521B1 (ko) * | 2008-02-01 | 2011-06-08 | 주식회사 인트론바이오테크놀로지 | 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물 |
| WO2011070440A2 (en) | 2009-12-10 | 2011-06-16 | Ottawa Hospital Research Institute | Oncolytic rhabdovirus |
| EP2718427B1 (de) | 2011-06-08 | 2017-01-11 | Children's Hospital of Eastern Ontario Research Institute Inc. | Zusammensetzungen zur behandlung von glioblastomen |
| EP2859111B1 (de) * | 2012-06-08 | 2020-07-08 | Ionian Technologies, LLC | Nukleinsäureamplifizierungen |
| JP2016513115A (ja) | 2013-02-21 | 2016-05-12 | チルドレンズ ホスピタル オブ イースタン オンタリオ リサーチ インスティチュート インコーポレイテッド | ワクチン組成物 |
| EP3099810B1 (de) | 2014-01-31 | 2019-05-01 | Qiagen GmbH | Kationen-chelator-warmstart |
| US10301660B2 (en) | 2015-03-30 | 2019-05-28 | Takara Bio Usa, Inc. | Methods and compositions for repair of DNA ends by multiple enzymatic activities |
| WO2018136404A1 (en) * | 2017-01-19 | 2018-07-26 | Asuragen, Inc. | Methods of rna amplification |
| GB201812192D0 (en) | 2018-07-26 | 2018-09-12 | Ttp Plc | Variable temperature reactor, heater and control circuit for the same |
| CN110846724B (zh) * | 2019-12-03 | 2023-04-28 | 江西海普洛斯医学检验实验室有限公司 | 构建mRNA链特异文库的方法及试剂盒 |
| JP7580877B2 (ja) * | 2020-03-30 | 2024-11-12 | 国立研究開発法人産業技術総合研究所 | 核酸増幅用組成物 |
| CN113045410A (zh) * | 2021-03-29 | 2021-06-29 | 华东理工大学 | 一种双环降二萜类化合物及其合成基因及制备方法 |
| EP4074839A1 (de) | 2021-04-16 | 2022-10-19 | Biotype GmbH | Optimierte oligonukleotid-tx-sonde für eine multiplexing-analyse von nukleinsäuren und ein multiplexing-verfahren |
| EP4350002A1 (de) | 2022-10-07 | 2024-04-10 | Biotype GmbH | Détection d'analytes moléculaires basée sur la concurrence de sondes sur mesure |
| DE102023124346A1 (de) | 2023-09-09 | 2025-03-13 | Biotype Gmbh | Verfahren zur Detektion des Endometriumkarzinoms Subtyp CN hoch |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1338457C (en) * | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
-
1990
- 1990-12-21 WO PCT/US1990/007641 patent/WO1991009944A2/en not_active Ceased
- 1990-12-21 AT AT91904087T patent/ATE151112T1/de not_active IP Right Cessation
- 1990-12-21 JP JP3504344A patent/JP2968585B2/ja not_active Expired - Lifetime
- 1990-12-21 CA CA002071213A patent/CA2071213C/en not_active Expired - Lifetime
- 1990-12-21 ES ES91904087T patent/ES2100945T3/es not_active Expired - Lifetime
- 1990-12-21 DK DK91904087.3T patent/DK0506889T3/da active
- 1990-12-21 AU AU72444/91A patent/AU656315B2/en not_active Expired
- 1990-12-21 EP EP91904087A patent/EP0506889B1/de not_active Expired - Lifetime
- 1990-12-21 DE DE69030386T patent/DE69030386T2/de not_active Expired - Lifetime
-
1997
- 1997-06-24 GR GR970401505T patent/GR3023862T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2071213A1 (en) | 1991-06-23 |
| DE69030386T2 (de) | 1997-10-09 |
| DE69030386D1 (de) | 1997-05-07 |
| DK0506889T3 (da) | 1997-09-22 |
| WO1991009944A2 (en) | 1991-07-11 |
| EP0506889A1 (de) | 1992-10-07 |
| CA2071213C (en) | 2000-05-02 |
| AU7244491A (en) | 1991-07-24 |
| JP2968585B2 (ja) | 1999-10-25 |
| GR3023862T3 (en) | 1997-09-30 |
| EP0506889B1 (de) | 1997-04-02 |
| AU656315B2 (en) | 1995-02-02 |
| JPH05505105A (ja) | 1993-08-05 |
| ES2100945T3 (es) | 1997-07-01 |
| WO1991009944A3 (en) | 1991-08-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EELA | Cancelled due to lapse of time |