ATE154074T1 - Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniae - Google Patents
Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniaeInfo
- Publication number
- ATE154074T1 ATE154074T1 AT91904924T AT91904924T ATE154074T1 AT E154074 T1 ATE154074 T1 AT E154074T1 AT 91904924 T AT91904924 T AT 91904924T AT 91904924 T AT91904924 T AT 91904924T AT E154074 T1 ATE154074 T1 AT E154074T1
- Authority
- AT
- Austria
- Prior art keywords
- dna polymerase
- purification
- preparation
- streptococcus pneumoniae
- pneumoniae
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000012634 fragment Substances 0.000 title abstract 2
- 102000004594 DNA Polymerase I Human genes 0.000 title 1
- 108010017826 DNA Polymerase I Proteins 0.000 title 1
- 241000193998 Streptococcus pneumoniae Species 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 229940031000 streptococcus pneumoniae Drugs 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- 230000000694 effects Effects 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 241000588724 Escherichia coli Species 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108060002716 Exonuclease Proteins 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 102000013165 exonuclease Human genes 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES9000542A ES2023538A6 (es) | 1990-02-23 | 1990-02-23 | Procedimiento de obtencion y purificacion del fragmento carboxilo-terminal de la dna polimerasa i de streptococcus pneumoniae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE154074T1 true ATE154074T1 (de) | 1997-06-15 |
Family
ID=8266083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT91904924T ATE154074T1 (de) | 1990-02-23 | 1991-02-13 | Verfahren zur herstellung und reinigung des carboxyterminalen fragments der dna-polymerase i von streptococcus pneumoniae |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP0470230B1 (de) |
| AT (1) | ATE154074T1 (de) |
| DE (1) | DE69126379T2 (de) |
| ES (2) | ES2023538A6 (de) |
| WO (1) | WO1991013153A1 (de) |
-
1990
- 1990-02-23 ES ES9000542A patent/ES2023538A6/es not_active Expired - Lifetime
-
1991
- 1991-02-13 DE DE69126379T patent/DE69126379T2/de not_active Expired - Fee Related
- 1991-02-13 EP EP91904924A patent/EP0470230B1/de not_active Expired - Lifetime
- 1991-02-13 AT AT91904924T patent/ATE154074T1/de active
- 1991-02-13 WO PCT/ES1991/000010 patent/WO1991013153A1/es not_active Ceased
-
1996
- 1996-05-31 ES ES09601205A patent/ES2095195A6/es not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0470230A1 (de) | 1992-02-12 |
| ES2023538A6 (es) | 1992-01-16 |
| DE69126379D1 (de) | 1997-07-10 |
| EP0470230B1 (de) | 1997-06-04 |
| ES2095195A6 (es) | 1997-02-01 |
| WO1991013153A1 (es) | 1991-09-05 |
| DE69126379T2 (de) | 1997-11-06 |
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