ATE162891T1 - Verfahren und zusammensetzungen für enzymkomplimentationassays unter verwendung der omega-region der beta-galactosidase - Google Patents

Verfahren und zusammensetzungen für enzymkomplimentationassays unter verwendung der omega-region der beta-galactosidase

Info

Publication number: ATE162891T1
Authority: AT; Austria
Prior art keywords: beta; galactosidase; enzyme; omega; compositions
Prior art date: 1991-05-15

Application number

AT92304354T

Other languages

English (en)

Inventor

Mark Krevolin

David Kates

Original Assignee

Boehringer Mannheim Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-05-15

Filing date

1992-05-14

Publication date

1998-02-15

1992-05-14 Application filed by Boehringer Mannheim Corp filed Critical Boehringer Mannheim Corp

1998-02-15 Application granted granted Critical

1998-02-15 Publication of ATE162891T1 publication Critical patent/ATE162891T1/de

Links

238000000034 method Methods 0.000 title abstract 4
102000005936 beta-Galactosidase Human genes 0.000 title abstract 3
108010005774 beta-Galactosidase Proteins 0.000 title abstract 3
102000004190 Enzymes Human genes 0.000 title abstract 2
108090000790 Enzymes Proteins 0.000 title abstract 2
238000003556 assay Methods 0.000 title abstract 2
239000000203 mixture Substances 0.000 title abstract 2
229920001184 polypeptide Polymers 0.000 abstract 2
102000004196 processed proteins & peptides Human genes 0.000 abstract 2
108090000765 processed proteins & peptides Proteins 0.000 abstract 2
230000006820 DNA synthesis Effects 0.000 abstract 1
108020004511 Recombinant DNA Proteins 0.000 abstract 1
239000012491 analyte Substances 0.000 abstract 1
230000015572 biosynthetic process Effects 0.000 abstract 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 1
230000003197 catalytic effect Effects 0.000 abstract 1
238000003173 enzyme complementation Methods 0.000 abstract 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
239000000126 substance Substances 0.000 abstract 1
238000003786 synthesis reaction Methods 0.000 abstract 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Hematology (AREA)
Immunology (AREA)
Urology & Nephrology (AREA)
Cell Biology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Microbiology (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Peptides Or Proteins (AREA)

AT92304354T 1991-05-15 1992-05-14 Verfahren und zusammensetzungen für enzymkomplimentationassays unter verwendung der omega-region der beta-galactosidase ATE162891T1 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US70054991A	1991-05-15	1991-05-15

Publications (1)

Publication Number	Publication Date
ATE162891T1 true ATE162891T1 (de)	1998-02-15

Family

ID=24813926

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT92304354T ATE162891T1 (de)	1991-05-15	1992-05-14	Verfahren und zusammensetzungen für enzymkomplimentationassays unter verwendung der omega-region der beta-galactosidase

Country Status (8)

Country	Link
US (1)	US5362625A (de)
EP (1)	EP0514173B1 (de)
JP (1)	JP2944823B2 (de)
AT (1)	ATE162891T1 (de)
AU (1)	AU657532B2 (de)
CA (1)	CA2068190C (de)
DE (1)	DE69224231T2 (de)
ES (1)	ES2114548T3 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5763196A (en) *	1996-01-26	1998-06-09	Boehringer Mannheim Corporation	Assays using cross-linked polypeptide fragments of β-galactosidase
US5976857A (en) *	1996-01-26	1999-11-02	Boehringer Mannheim Corporation	Cross-linked polypeptide fragments of β-galactosidase
ATE211480T1 (de) *	1996-01-26	2002-01-15	Roche Diagnostics Corp	Bismaleinsäureimid-vernetzer
AU783091B2 (en) *	1997-01-31	2005-09-22	Odyssey Thera, Inc.	Protein fragment complementation assays to detect biomolecular interactions
CA2196496A1 (en)	1997-01-31	1998-07-31	Stephen William Watson Michnick	Protein fragment complementation assay for the detection of protein-protein interactions
US6342345B1 (en) *	1997-04-02	2002-01-29	The Board Of Trustees Of The Leland Stanford Junior University	Detection of molecular interactions by reporter subunit complementation
CA2205888A1 (en) *	1997-07-11	1999-01-11	John J. Priatel	Complementation trap
WO1999006537A1 (en) *	1997-07-30	1999-02-11	Boehringer Mannheim Corporation	Cross-linked polypeptide assay components
GB2344886B (en) *	1999-03-10	2000-11-01	Medical Res Council	Selection of intracellular immunoglobulins
US8148110B2 (en) *	1999-03-15	2012-04-03	The Board Of Trustees Of The Leland Stanford Junior University	Detection of molecular interactions by β-lactamase reporter fragment complementation
US8586294B2 (en) *	2004-05-18	2013-11-19	The Board Of Trustees Of The Leland Stanford Junior University	Detection of protein translocation by beta-galactosidase reporter fragment complementation
WO2007050584A2 (en) *	2005-10-24	2007-05-03	Discoverx, Inc.	Detection of intracellular enzyme complex
EP1994178A4 (de) *	2006-03-13	2009-11-11	Univ Leland Stanford Junior	Nachweis von molekularen wechselwirkungen unter verwendung eines enzymkomplementationsreportersystems mit verringerter affinität
US20070254338A1 (en) *	2006-04-24	2007-11-01	Amgen Inc.	Method for making recombinant protein using complementation dependent DHFR mutants
US8101373B2 (en) *	2007-10-12	2012-01-24	Discoverx Corporation	β-galactosidase donor fragments
JP2014505059A (ja)	2011-01-18	2014-02-27	ジェネラルアトミクス	ヒドロラーゼ酵素基質およびその使用
WO2012178079A1 (en)	2011-06-23	2012-12-27	Discoverx Corporation	Monitoring protein trafficking using beta-galactosidase reporter fragment complementation
WO2014066404A2 (en) *	2012-10-22	2014-05-01	Life Technologies Corporation	System and method for visualization of optimized protein expression
CN110386986B (zh) *	2018-06-22	2024-01-26	深圳琅技生命科技有限公司	人工改造蛋白质及其构建方法与应用
WO2022056020A1 (en) *	2020-09-09	2022-03-17	The Regents Of The University Of California	Systems and methods for the amplified detection of molecules on microparticles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4378428A (en) *	1981-03-30	1983-03-29	Baker Instruments Corporation	Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
US4708929A (en) *	1984-10-29	1987-11-24	Microgenics Corporation	Methods for protein binding enzyme complementation assays
EP0199801B1 (de) *	1984-10-29	1993-08-25	MICROGENICS CORPORATION (a Delaware corporation)	Ergänzungstestverfahren von proteine bindenden enzymen
ES2093026T3 (es) *	1989-05-05	1996-12-16	Microgenics Corp	Procedimiento de ensayo de complementacion enzimatico de enlace e proteinas.
US5244785A (en) *	1991-02-01	1993-09-14	Microgenics Corporation	Determination of high molecular weight analytes using a β-galactosidase complementation assay

1992
- 1992-05-07 CA CA002068190A patent/CA2068190C/en not_active Expired - Fee Related
- 1992-05-14 AU AU16291/92A patent/AU657532B2/en not_active Ceased
- 1992-05-14 AT AT92304354T patent/ATE162891T1/de not_active IP Right Cessation
- 1992-05-14 DE DE69224231T patent/DE69224231T2/de not_active Expired - Fee Related
- 1992-05-14 ES ES92304354T patent/ES2114548T3/es not_active Expired - Lifetime
- 1992-05-14 EP EP92304354A patent/EP0514173B1/de not_active Expired - Lifetime
- 1992-05-15 JP JP4123368A patent/JP2944823B2/ja not_active Expired - Fee Related
1993
- 1993-05-25 US US08/067,342 patent/US5362625A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE69224231D1 (de)	1998-03-05
EP0514173A3 (en)	1994-07-06
AU1629192A (en)	1993-03-11
DE69224231T2 (de)	1998-07-23
CA2068190A1 (en)	1992-11-16
CA2068190C (en)	1996-12-17
US5362625A (en)	1994-11-08
ES2114548T3 (es)	1998-06-01
JPH05276959A (ja)	1993-10-26
AU657532B2 (en)	1995-03-16
JP2944823B2 (ja)	1999-09-06
EP0514173A2 (de)	1992-11-19
EP0514173B1 (de)	1998-01-28

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Legal Events

Date	Code	Title	Description
1998-07-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties