ATE172542T1 - Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern - Google Patents

Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern

Info

Publication number
ATE172542T1
ATE172542T1 AT91918547T AT91918547T ATE172542T1 AT E172542 T1 ATE172542 T1 AT E172542T1 AT 91918547 T AT91918547 T AT 91918547T AT 91918547 T AT91918547 T AT 91918547T AT E172542 T1 ATE172542 T1 AT E172542T1
Authority
AT
Austria
Prior art keywords
enlarged
determination
test method
western blot
viral antibodies
Prior art date
Application number
AT91918547T
Other languages
English (en)
Inventor
Lily Chan
Yoke Wah Sum
May Fong Yin
Lee Fang Lim
Original Assignee
Diagnostic Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diagnostic Biotech Inc filed Critical Diagnostic Biotech Inc
Application granted granted Critical
Publication of ATE172542T1 publication Critical patent/ATE172542T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/42—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins
    • C07K16/4283—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against immunoglobulins against an allotypic or isotypic determinant on Ig
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/14011—Deltaretrovirus, e.g. bovine leukeamia virus
    • C12N2740/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2
    • G01N2333/162—HIV-1, HIV-2 env, e.g. gp160, gp110/120, gp41, V3, peptid T, DC4-Binding site
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/97—Test strip or test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/974—Aids related test

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • AIDS & HIV (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT91918547T 1990-08-16 1991-08-14 Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern ATE172542T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US56814490A 1990-08-16 1990-08-16

Publications (1)

Publication Number Publication Date
ATE172542T1 true ATE172542T1 (de) 1998-11-15

Family

ID=24270079

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91918547T ATE172542T1 (de) 1990-08-16 1991-08-14 Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern

Country Status (6)

Country Link
US (1) US5721095A (de)
EP (1) EP0564460B1 (de)
AT (1) ATE172542T1 (de)
AU (1) AU8849391A (de)
DE (1) DE69130396T2 (de)
WO (1) WO1992003579A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6544728B1 (en) * 1986-01-22 2003-04-08 Institut Pasteur Methods and kits for diagnosing human immunodeficiency virus type 2 (HIV-2), proteins of HIV-2, and vaccinating agents for HIV-2
US6054565A (en) 1986-03-03 2000-04-25 Institut Pasteur Nucleic Acids of HIV-2, Diagnostic Test Kit and Method using Nucleic Acid Probes of HIV-2
JPH11514872A (ja) * 1995-10-18 1999-12-21 ダーウィン モレキュラー コーポレイション ハイブリダイゼーション用の固体支持体を調製する方法および非特異的バックグラウンドを低減する方法
JP2001511362A (ja) 1997-07-22 2001-08-14 ラピジーン，インコーポレイテッド Ｍｓにより配列決定データを相関させるコンピュータ方法およびシステム
US6228128B1 (en) 1997-11-10 2001-05-08 Charlotte Johansen Antimicrobial activity of laccases
US20030073250A1 (en) * 1999-05-21 2003-04-17 Eric Henderson Method and apparatus for solid state molecular analysis
US6897015B2 (en) * 2000-03-07 2005-05-24 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of pathogens and biological materials
US7074553B2 (en) * 2000-11-28 2006-07-11 Rush University Medical Center Retrovirus associated with myelodysplastic syndromes and uses thereof
JP3523188B2 (ja) 2000-12-13 2004-04-26 富士写真フイルム株式会社 プローブ分子が固定された検出具の水性処理液
US20050239193A1 (en) * 2002-05-30 2005-10-27 Bioforce Nanosciences, Inc. Device and method of use for detection and characterization of microorganisms and microparticles
EP2309269B1 (de) 2004-09-08 2016-10-26 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Zusammensetzungen und verfahren zur detektion von hiv-1/hiv-2 infektion
WO2010126514A1 (en) * 2009-04-30 2010-11-04 Hewlett-Packard Development Company, L.P. An analysis system including a pre-selected combination of cromophore precursors or chromophores
EP2550362B1 (de) 2010-03-25 2017-01-04 Oregon Health&Science University Cmv-glycoproteine und rekombinante vektoren
CA2832109C (en) 2011-06-10 2021-07-06 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
EP2568289A3 (de) 2011-09-12 2013-04-03 International AIDS Vaccine Initiative Immunselektion von rekombinantem vesikulärem Stomatitisvirus mit Expression von HIV-1-Proteinen durch Breitbandneutralisierungs-Antikörper
US9402894B2 (en) 2011-10-27 2016-08-02 International Aids Vaccine Initiative Viral particles derived from an enveloped virus
PT2856163T (pt) 2012-05-30 2016-10-11 Bio-Rad Innovations Método para diagnóstico e diferenciação de infeções de hiv-2
EP2679596B1 (de) 2012-06-27 2017-04-12 International Aids Vaccine Initiative HIV-1 Env-proteinvariante
EP2848937A1 (de) 2013-09-05 2015-03-18 International Aids Vaccine Initiative Verfahren zur Identifizierung neuartiger HIV-1-Immunogene
US10058604B2 (en) 2013-10-07 2018-08-28 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
EP3069730A3 (de) 2015-03-20 2017-03-15 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere
EP3072901A1 (de) 2015-03-23 2016-09-28 International Aids Vaccine Initiative Lösliche hiv-1-hüllglykoproteintrimere

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4452901A (en) * 1980-03-20 1984-06-05 Ciba-Geigy Corporation Electrophoretically transferring electropherograms to nitrocellulose sheets for immuno-assays
US4520113A (en) * 1984-04-23 1985-05-28 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions
US9328391B1 (en) * 1984-08-22 2016-05-03 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Cloning and expression of HIV-1 DNA
US4629783A (en) * 1985-04-29 1986-12-16 Genetic Systems Corporation Synthetic antigen for the detection of AIDS-related disease
US4735896A (en) * 1986-03-04 1988-04-05 United Biomedical, Inc. Synthetic peptide and process of using same for the detection and diagnosis of AIDS and pre-AIDS conditions
US4918000A (en) * 1985-09-27 1990-04-17 Walter Schubert Multi-color labeling of different antigens in a biological system
AU6713287A (en) * 1986-01-06 1987-07-09 F. Hoffmann-La Roche & Co. Expression of htlv-iii gag-gene
ATE128738T1 (de) * 1987-07-13 1995-10-15 Verigen Inc Verfahren zum schnellen und empfindlichen nachweis von igm-antikörpern gegen retrovirale antigene.
CA1339006C (en) * 1987-08-05 1997-03-25 Howard B. Urnovitz Self-contained multi-immunoassay diagnostic system
US5039604A (en) * 1987-08-21 1991-08-13 Cellular Products, Inc. Test device and method of preparing same, assay kit and method for the simultaneous detection of two HTLV or HIV antibodies
ES2066887T3 (es) * 1989-02-03 1995-03-16 Abbott Lab Anticuerpo monoclonal para diferenciar individuos seropositivos hiv-2 de los hiv-1.

Also Published As

Publication number Publication date
HK1013849A1 (en) 1999-09-10
DE69130396T2 (de) 1999-06-24
DE69130396D1 (de) 1998-11-26
AU8849391A (en) 1992-03-17
EP0564460A1 (de) 1993-10-13
US5721095A (en) 1998-02-24
EP0564460A4 (en) 1994-06-29
WO1992003579A1 (en) 1992-03-05
EP0564460B1 (de) 1998-10-21

Similar Documents

Publication Publication Date Title
ATE172542T1 (de) Vergrössertes western blot format und ein immunotestverfahren für die bestimmung von viralen antikörpern
NZ219113A (en) Hiv peptide containing epitope of the env protein
NZ237666A (en) Hiv envelope (env) polypeptides, antibodies and compositions for treating aids
ES2016426A6 (es) Un procedimiento para detectar la presencia de anticuerpos frente a virus del sida en el suero humano.
PT649537E (pt) Imunoanalises para anticorpos anti-vhc utilizando antigenios com epitopos conformacionais
NO881151L (no) Syntetisk hiv-1-antigen.
ES8802457A1 (es) Un procedimiento para la preparacion de polipeptidos inmunogenicos
DE3584035D1 (de) Nachweis von menschlichem cytomegalovirus-spezifischem igm.
KR910019641A (ko) 사람 유두종 바이러스(hpv) 18의 단백질상의 혈청 반응성 에피토프
CA2111325A1 (en) Detection of mammalian immunodeficiency viruses
DE3579856D1 (de) Immunulogischer test zur bestimmung von gemeinsamen, bestimmenden hepatitis-b-antikoerpern.
SE9000595D0 (sv) New peptides, diagnostic antigens and an immunoassay method
ATE119203T1 (de) Monoklonale antikörper von mäusen gegen das gp41 protein von menschlichem immunmangelsyndrom- virus.
FI925321A0 (fi) Immunodiagnostiskt ledinflammationstest
ATE116660T1 (de) Künstliche peptid-zusammensetzungen mit immunoreaktiven antikörpern von htlv-1.
ATE308618T1 (de) Mimotopen der hypervariabele region des e2 glykoproteins von hcv und deren verwendungen
ATE108457T1 (de) Synthetische antigene zur bestimmung der aids- bezogenen, von lav-2 verursachten krankheiten.
CA2100586A1 (en) Htlv-i and htlv-ii peptide antigens and methods
DE69026875D1 (de) Immunobestimmung von menschlichem osteocalcin, reagenz und satz dafür
ES2061973T3 (es) Anticuerpos monoclonales que reconocen la region n-terminal de polipeptido natriuretico auricular, su preparacion y uso.
ES2061664T3 (es) Anticuerpos monoclonales que reconocen a alfa-hanp y correspondientes hibridomas, su preparacion y uso.
ATE138078T1 (de) Analysemethode für infektiöse pferdeanämie-viren (eia-virus)
Karetnyĭ et al. Study of human and simian hepatitis A virus isolated by an immunoenzyme method using polyclonal and monoclonal antibodies

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties