ATE172740T1 - Ein enzymatisches verfahren zur herstellung eines gewünschten proteins aus einem am aminoterminus verlängerten protein - Google Patents
Ein enzymatisches verfahren zur herstellung eines gewünschten proteins aus einem am aminoterminus verlängerten proteinInfo
- Publication number
- ATE172740T1 ATE172740T1 AT95918545T AT95918545T ATE172740T1 AT E172740 T1 ATE172740 T1 AT E172740T1 AT 95918545 T AT95918545 T AT 95918545T AT 95918545 T AT95918545 T AT 95918545T AT E172740 T1 ATE172740 T1 AT E172740T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- producing
- pct
- enzymatic process
- amino terminus
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- -1 AMINO Chemical class 0.000 title 1
- 102000004400 Aminopeptidases Human genes 0.000 abstract 2
- 108090000915 Aminopeptidases Proteins 0.000 abstract 2
- NPWMTBZSRRLQNJ-VKHMYHEASA-N (3s)-3-aminopiperidine-2,6-dione Chemical compound N[C@H]1CCC(=O)NC1=O NPWMTBZSRRLQNJ-VKHMYHEASA-N 0.000 abstract 1
- 102000003642 glutaminyl-peptide cyclotransferase Human genes 0.000 abstract 1
- 108010081484 glutaminyl-peptide cyclotransferase Proteins 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK53394 | 1994-05-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE172740T1 true ATE172740T1 (de) | 1998-11-15 |
Family
ID=8094618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95918545T ATE172740T1 (de) | 1994-05-09 | 1995-05-09 | Ein enzymatisches verfahren zur herstellung eines gewünschten proteins aus einem am aminoterminus verlängerten protein |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5783413A (de) |
| EP (1) | EP0759931B1 (de) |
| AT (1) | ATE172740T1 (de) |
| AU (1) | AU2445495A (de) |
| DE (1) | DE69505660T2 (de) |
| DK (1) | DK0759931T3 (de) |
| WO (1) | WO1995030685A1 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6743600B1 (en) | 1999-04-30 | 2004-06-01 | Monsanto Technologies Llc | Method of removing N-terminal alanine residues from polypeptides with Aeromonas aminopeptidase |
| US6794159B1 (en) * | 1999-04-30 | 2004-09-21 | Pharmacia Corporation | Method of removing n-terminal alanine residues from polypeptides with aeromonas aminopeptidase |
| US7736875B2 (en) | 2000-09-08 | 2010-06-15 | Prozymex A/S | Dipeptidyl peptidase I crystal structure and its uses |
| US20030143664A1 (en) * | 2001-12-03 | 2003-07-31 | Diversa Corporation | Products of manufacture and processes for peptide synthesis |
| DE602004032379D1 (de) | 2003-12-19 | 2011-06-01 | Novo Nordisk As | Prozessierung von peptiden und proteinen |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL60184A (en) * | 1979-05-31 | 1984-05-31 | Schering Ag | Process for the specific cleavage of protein sequences from proteins |
| DK55685A (da) * | 1985-02-07 | 1986-08-08 | Nordisk Gentofte | Enzym eller enzymkompleks med proteolytisk aktivitet |
| US4745051A (en) * | 1983-05-27 | 1988-05-17 | The Texas A&M University System | Method for producing a recombinant baculovirus expression vector |
| WO1986001229A1 (en) * | 1984-08-16 | 1986-02-27 | Bio-Technology General Corp. | Method of removing n-terminal amino acid residues from eucaryotic polypetide analogs and polypeptides produced thereby |
| US5141922A (en) * | 1985-02-22 | 1992-08-25 | Monsanto Company | Biologically active proteins and a method for their use |
| US5013662A (en) * | 1985-09-20 | 1991-05-07 | Cetus Corporation | Bacterial methionine n-terminal peptidase |
| US5028694A (en) * | 1985-12-03 | 1991-07-02 | Solvay & Cie, S.A. | Antigenic proteins and vaccines containing them for prevention of coccidiosis caused by eimeria Eimeria necatrix and Eimeria tenella |
| US5126249A (en) * | 1989-05-09 | 1992-06-30 | Eli Lilly And Company | Enzymatic removal of a protein amino-terminal sequence |
| DE69120409T2 (de) * | 1990-04-09 | 1996-11-28 | Upjohn Co | Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte |
-
1995
- 1995-05-09 WO PCT/DK1995/000184 patent/WO1995030685A1/en not_active Ceased
- 1995-05-09 EP EP95918545A patent/EP0759931B1/de not_active Expired - Lifetime
- 1995-05-09 DE DE69505660T patent/DE69505660T2/de not_active Expired - Fee Related
- 1995-05-09 US US08/737,757 patent/US5783413A/en not_active Expired - Fee Related
- 1995-05-09 AT AT95918545T patent/ATE172740T1/de not_active IP Right Cessation
- 1995-05-09 AU AU24454/95A patent/AU2445495A/en not_active Abandoned
- 1995-05-09 DK DK95918545T patent/DK0759931T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| DE69505660T2 (de) | 1999-06-17 |
| DE69505660D1 (de) | 1998-12-03 |
| AU2445495A (en) | 1995-11-29 |
| DK0759931T3 (da) | 1999-07-12 |
| EP0759931B1 (de) | 1998-10-28 |
| WO1995030685A1 (en) | 1995-11-16 |
| EP0759931A1 (de) | 1997-03-05 |
| US5783413A (en) | 1998-07-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE59711073D1 (de) | Verfahren zur herstellung von polyalkenaminen | |
| DE60212193D1 (de) | Verfahren zur verbesserung der reproduktionsleistung bei geflügel | |
| ATE392430T1 (de) | Verfahren zur herstellung von dipeptiden | |
| ATE226949T1 (de) | Verfahren zur herstellung von tolterodin | |
| DE59601822D1 (de) | Verfahren zur herstellung von weizenproteinhydrolysaten | |
| DE69941701D1 (de) | Mutanten von flt3-l und ihre verwendungen | |
| DE50208306D1 (de) | Verfahren zur Herstellung von nicht-proteinogenen L-Aminosäuren | |
| ATE371741T1 (de) | Systeme zur massenherstellung von proteinen oder peptiden durch mikroorganismen der gattung humicola | |
| ATE172740T1 (de) | Ein enzymatisches verfahren zur herstellung eines gewünschten proteins aus einem am aminoterminus verlängerten protein | |
| DE69621835D1 (de) | Verfahren zur herstellung von arylhydrazin durch reduktion von azo-derivate | |
| ATE279931T1 (de) | Verwendung von enzymatischen austerfleischhydrolysaten zur herstellung von antiradikalischen zusammensetzungen | |
| ATE214686T1 (de) | Verfahren zur herstellung von fluorverbindungen aus den entsprechenden aminen | |
| DE59606567D1 (de) | Neuer Mikroorganismus, dessen Verwendung und Verfahren zur Herstellung von L-Alpha-Aminosäuren | |
| DE50112449D1 (de) | Verfahren zur herstellung von 3,3-diarylpropylaminen | |
| ATE271561T1 (de) | Verfahren zur herstellung von einer amid verbindung | |
| DE58909067D1 (de) | Verfahren zur herstellung von l-aminosäuren und aminosäure-amiden. | |
| ATE525911T1 (de) | Verfahren zur verbesserung der fleischqualität von fisch,proteinhydrolysat und verfahren zur herstellung eines proteinhydrolysats | |
| DE59603295D1 (de) | Verfahren zur herstellung von dolastatin 15 und deren zwischenprodukten | |
| DE50208458D1 (de) | Verfahren zur Herstellung von Lithiumamid | |
| ATE522604T1 (de) | Verfahren zur herstellung von proteinen durch elektropulsierung | |
| DE69505765D1 (de) | Verfahren zur Herstellung von (Co)chloroprenkautschuk | |
| ATE415417T1 (de) | Luxr-gen aus coryneformen bakterien und ein verfahren zur herstellung von l-aminosäuren | |
| ATE285478T1 (de) | Zellulosefilme zur durchfuehrung von screening- verfahren | |
| ATE248807T1 (de) | Verfahren zur herstellung von n-hydrocarbyl- substituierten amiden | |
| ATE288445T1 (de) | Herstellung von n-(n-(3,3-dimethylbutyl)-l-alpha- aspartyl)-l-phenylalanin-1-methylester durch verwendung vom l-alpha-aspartyl-l-phenylalanin-1- methylester als vorläufer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |