ATE174121T1 - PROTEIN NUCLEIC ACID PROBE AND IMMUNOASSAY USING THE SAME - Google Patents

PROTEIN NUCLEIC ACID PROBE AND IMMUNOASSAY USING THE SAME

Info

Publication number
ATE174121T1
ATE174121T1 AT91908991T AT91908991T ATE174121T1 AT E174121 T1 ATE174121 T1 AT E174121T1 AT 91908991 T AT91908991 T AT 91908991T AT 91908991 T AT91908991 T AT 91908991T AT E174121 T1 ATE174121 T1 AT E174121T1
Authority
AT
Austria
Prior art keywords
nucleic acid
immunoassay
same
acid probe
protein nucleic
Prior art date
Application number
AT91908991T
Other languages
German (de)
Inventor
Michael S Urdea
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of ATE174121T1 publication Critical patent/ATE174121T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
    • G01N2333/04—Varicella-zoster virus
    • G01N2333/045—Cytomegalovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

In one aspect of this invention, various protein/nucleic acid hybrid probes are described which can be used to amplify the detectable signal in immunoassays. The protein moiety is capable of functioning either as an antibody or an antigen. The nucleic acid moiety serves as a signal amplifier. In another aspect, various methods of amplifying the detectable signal in immunoassays by use of the hybrid probes and related polynucleotide probes are disclosed.
AT91908991T 1990-05-04 1991-05-06 PROTEIN NUCLEIC ACID PROBE AND IMMUNOASSAY USING THE SAME ATE174121T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US51921290A 1990-05-04 1990-05-04

Publications (1)

Publication Number Publication Date
ATE174121T1 true ATE174121T1 (en) 1998-12-15

Family

ID=24067357

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91908991T ATE174121T1 (en) 1990-05-04 1991-05-06 PROTEIN NUCLEIC ACID PROBE AND IMMUNOASSAY USING THE SAME

Country Status (14)

Country Link
EP (1) EP0528870B1 (en)
JP (1) JP3034304B2 (en)
KR (1) KR0171617B1 (en)
AT (1) ATE174121T1 (en)
AU (1) AU659798B2 (en)
BG (1) BG61315B1 (en)
CA (1) CA2081455A1 (en)
DE (1) DE69130564T2 (en)
HU (1) HUT64623A (en)
IE (1) IE911534A1 (en)
PT (1) PT97580B (en)
RO (1) RO113496B1 (en)
RU (1) RU2107730C1 (en)
WO (1) WO1991017442A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE184321T1 (en) * 1990-01-10 1999-09-15 Chiron Diagnostics Corp DNA-DEPENDENT RNA POLYMERASE TRANSCRIPTS AS REPORTER MOLECULES FOR SIGNAL AMPLIFICATION IN NUCLEIC ACID HYBRIDIZATION STUDIES
US5629153A (en) * 1990-01-10 1997-05-13 Chiron Corporation Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
EP0648281A4 (en) * 1991-09-10 1997-06-04 Jack D Love Dna/rna target and signal amplification.
JPH05149949A (en) * 1991-11-26 1993-06-15 Nisshin Flour Milling Co Ltd New trace substance measurement method
AU3618593A (en) * 1992-02-04 1993-09-01 E.I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
WO1993024658A1 (en) * 1992-05-29 1993-12-09 Gen Trak, Inc. Signal amplification probe and methods of use
ES2044784B1 (en) * 1992-06-12 1994-09-01 Inia PROCEDURE FOR THE DETECTION AND IDENTIFICATION OF VIRAL AND SUBVIRAL PATHOGENS.
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
WO1994026932A1 (en) * 1993-05-13 1994-11-24 United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nucleic acid tagged immunoassay
EP0778777A4 (en) * 1994-08-22 1999-01-27 Univ Pennsylvania A method of sequencing proteins by epitope ordering and protein restriction mapping
US5753787A (en) * 1995-04-10 1998-05-19 Yale University Nucleic acids encoding ancylostoma secreted protein
SE504798C2 (en) 1995-06-16 1997-04-28 Ulf Landegren Immunoassay and test kits with two reagents that can be cross-linked if adsorbed to the analyte
AU2980397A (en) 1996-06-10 1998-01-07 Laboratory Of Molecular Biophotonics Highly sensitive fluorescent immunoassay
US5922553A (en) * 1996-11-21 1999-07-13 Trustees Of The University Of Pennsylvania Method of detecting protein by immuno RNA
GB9718921D0 (en) * 1997-09-05 1997-11-12 Brax Genomics Ltd Catalytically generated mass labels
EP1098990B1 (en) * 1998-12-10 2006-04-12 Daniel P. Cashman Blocked-polymerase polynucleotide immunoassay method and kit
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
EP1395805A4 (en) * 2001-06-11 2005-03-09 Illumina Inc Multiplexed detection methods
AU2002347333A1 (en) * 2001-12-07 2003-06-17 The University Of Liverpool Immuno polymerase chain reaction assay
CN117288934A (en) * 2013-03-13 2023-12-26 中尺度技术有限责任公司 Improved measurement method
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
KR102757668B1 (en) 2014-05-15 2025-01-21 메소 스케일 테크놀러지즈, 엘엘시 Improved assay methods
CN120098057A (en) 2019-03-01 2025-06-06 中尺度技术有限责任公司 Electrochemiluminescent labeled probes for use in immunological methods, methods of using such probes, and kits containing such probes
CN114544965B (en) * 2020-11-11 2023-04-28 艾克发(北京)生物技术有限公司 Multiple signal amplification system and application thereof in immunoadsorption competition method detection
ES3030311T3 (en) 2021-05-25 2025-06-27 Cavidi Ab Method for sensitive analyte detection assays and kits therefor
AU2022304570A1 (en) 2021-06-28 2024-01-18 Meso Scale Technologies, Llc. Methods, compositions, and kits for assay signal amplification
CN114277090A (en) * 2021-12-17 2022-04-05 宁波熙宁检测技术有限公司 Detection method and detection kit for AAV8 neutralizing antibody

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4299916A (en) * 1979-12-26 1981-11-10 Syva Company Preferential signal production on a surface in immunoassays
FI63596C (en) * 1981-10-16 1983-07-11 Orion Yhtymae Oy MICROBIA DIAGNOSIS FOERFARANDE SOM GRUNDAR SIG PAO SKIKTSHYBRIDISERING AV NUCLEINSYROR OCH VID FOERFARANDET ANVAENDA KOMBINATIONER AV REAGENSER
US4556643A (en) * 1982-07-26 1985-12-03 Agracetus Assay method and probe for polynucleotide sequences
US4748111A (en) * 1984-03-12 1988-05-31 Molecular Diagnostics, Inc. Nucleic acid-protein conjugate used in immunoassay
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
US4828979A (en) * 1984-11-08 1989-05-09 Life Technologies, Inc. Nucleotide analogs for nucleic acid labeling and detection
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
CA1339351C (en) * 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
JP2939485B2 (en) * 1988-09-08 1999-08-25 ザ・ソーク・インスチュート・フォーバイオロジカル・スタディーズ Amplification / detection system based on replicated RNA

Also Published As

Publication number Publication date
KR0171617B1 (en) 1999-05-01
WO1991017442A1 (en) 1991-11-14
DE69130564T2 (en) 1999-07-29
EP0528870A1 (en) 1993-03-03
RU2107730C1 (en) 1998-03-27
IE911534A1 (en) 1991-11-06
JP3034304B2 (en) 2000-04-17
PT97580A (en) 1992-03-31
CA2081455A1 (en) 1991-11-05
RO113496B1 (en) 1998-07-30
EP0528870B1 (en) 1998-12-02
HU9203445D0 (en) 1993-01-28
HUT64623A (en) 1994-01-28
EP0528870A4 (en) 1993-06-09
BG97041A (en) 1993-12-24
AU7791291A (en) 1991-11-27
PT97580B (en) 1998-08-31
BG61315B1 (en) 1997-05-30
AU659798B2 (en) 1995-06-01
DE69130564D1 (en) 1999-01-14
JPH06506768A (en) 1994-07-28

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