ATE189478T1 - Für die reinigung bestimmte klonierung von peptiden - Google Patents

Für die reinigung bestimmte klonierung von peptiden

Info

Publication number
ATE189478T1
ATE189478T1 AT92902529T AT92902529T ATE189478T1 AT E189478 T1 ATE189478 T1 AT E189478T1 AT 92902529 T AT92902529 T AT 92902529T AT 92902529 T AT92902529 T AT 92902529T AT E189478 T1 ATE189478 T1 AT E189478T1
Authority
AT
Austria
Prior art keywords
product peptide
purification
carbonic anhydrase
protein
binding protein
Prior art date
Application number
AT92902529T
Other languages
English (en)
Inventor
Thomas R Coolidge
Fred Wagner
Heeke Gino Van
Sheldon M Schuster
Jay Stout
Dwane E Wylie
Original Assignee
Bionebraska Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bionebraska Inc filed Critical Bionebraska Inc
Application granted granted Critical
Publication of ATE189478T1 publication Critical patent/ATE189478T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/595—Gastrins; Cholecystokinins [CCK]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/14—Angiotensins: Related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Vascular Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
AT92902529T 1990-07-16 1991-06-24 Für die reinigung bestimmte klonierung von peptiden ATE189478T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/552,810 US5595887A (en) 1990-07-16 1990-07-16 Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
PCT/US1991/004511 WO1992001707A1 (en) 1990-07-16 1991-06-24 Purification directed cloning of peptides

Publications (1)

Publication Number Publication Date
ATE189478T1 true ATE189478T1 (de) 2000-02-15

Family

ID=24206900

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92902529T ATE189478T1 (de) 1990-07-16 1991-06-24 Für die reinigung bestimmte klonierung von peptiden

Country Status (12)

Country Link
US (2) US5595887A (de)
EP (1) EP0539530B1 (de)
JP (1) JP3129437B2 (de)
AT (1) ATE189478T1 (de)
AU (1) AU662302B2 (de)
CA (1) CA2087261C (de)
DE (1) DE69131961D1 (de)
DK (1) DK0539530T3 (de)
IE (1) IE912464A1 (de)
NZ (1) NZ238812A (de)
WO (1) WO1992001707A1 (de)
ZA (1) ZA915307B (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639624A (en) * 1989-03-14 1997-06-17 Board Of Regents Of The University Of Nebraska Monoclonal antibodies specific for metallic cations and method therefor
US5512459A (en) * 1993-07-20 1996-04-30 Bionebraska, Inc. Enzymatic method for modification or recombinant polypeptides
WO1996017942A1 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of peptides using recombinant fusion protein constructs
WO1996017941A2 (en) * 1994-12-07 1996-06-13 Bionebraska, Inc. Production of c-terminal amidated peptides from recombinant protein constructs
US6111079A (en) * 1995-06-05 2000-08-29 Bionebraska, Inc. Lead binding polypeptides and nucleotides coding therefore
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US5962270A (en) 1996-02-06 1999-10-05 Bionebraska, Inc. Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs
EP0832972A3 (de) * 1996-09-23 1999-11-17 Givaudan-Roure (International) S.A. Expression von Peptide mit geschmaksverleihender Eigenschaften
ES2308800T3 (es) 1997-01-08 2008-12-01 Invitrogen Corporation Metodos para la produccion de proteinas.
US6395965B1 (en) 1997-03-21 2002-05-28 Restoragen, Inc. Plant containing a gene construct comprising a chlorella virus promoter and a lac operator
US5846774A (en) 1997-03-21 1998-12-08 Bionebraska, Inc. Chlorella virus promoters
GB9716790D0 (en) 1997-08-07 1997-10-15 Creative Peptides Sweden Ab Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof
US6303340B1 (en) * 1998-07-10 2001-10-16 Scios Inc. Method for producing a peptide with a pI above 8 or below 5
US6461834B1 (en) 1998-11-06 2002-10-08 Bionebraska, Inc. Clostripain catalyzed amidation of peptides
US6852685B1 (en) 1999-08-17 2005-02-08 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof to promote neuronal cell differentiation
EP1218018A4 (de) * 1999-08-17 2005-06-01 Univ Texas Verwendung von colostrinin, seine peptidbestandteile und ihre analogen als regulatoren bei oxidativem stress
US6903068B1 (en) 1999-08-17 2005-06-07 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof for inducing cytokines
US7119064B2 (en) * 1999-08-17 2006-10-10 Board Of Regents, The University Of Texas System Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules
US20030044420A1 (en) * 1999-09-24 2003-03-06 Mccormick Alison A. Self antigen vaccines for treating B cell lymphomas and other cancers
US7084256B2 (en) * 1999-09-24 2006-08-01 Large Scale Biology Corporation Self antigen vaccines for treating B cell lymphomas and other cancers
ES2252187T3 (es) * 2000-01-20 2006-05-16 Regents Of The University Of Minnesota Peptidos con actividad antibacteriana.
DE60140864D1 (de) * 2000-04-13 2010-02-04 Thomas N Wight Therapeutische zusammenfassungen und verfahren zur modulierung von v3, eine isoform von versican
EP1472276A4 (de) * 2001-02-28 2007-05-09 Keith M Skubitz Kleine, zur modulation der funktion von mitgliedern der cd66 (ceacam)-familie fähige peptide
CN1255548C (zh) 2001-06-15 2006-05-10 霍夫曼-拉罗奇有限公司 gp41片段的乙酰化
CN1363654A (zh) * 2001-07-19 2002-08-14 上海华谊生物技术有限公司 生产促胰岛素分泌肽glp－1(7－36)的基因工程菌以及生产glp－1(7－36)的方法
WO2003050540A2 (en) * 2001-12-05 2003-06-19 Large Scale Biology Corporation Flexible method and apparatus for high throughput production and purification of multiple proteins
WO2003070751A2 (en) 2002-02-20 2003-08-28 Regents Of The University Of Minnesota Partial peptide mimetics and methods
DE10221411B4 (de) * 2002-05-14 2004-07-08 GSF-Forschungszentrum für Umwelt und Gesundheit GmbH Rekombinantes Fowlpox-Virus
WO2003099848A2 (en) 2002-05-24 2003-12-04 Restoragen Inc. Method for universal enzymatic production of bioactive peptides
AU2003239863A1 (en) * 2002-05-24 2003-12-12 Restoragen Inc. Method for enzymatic production of glp-1 (7-36) amide peptides
EP1572720A4 (de) * 2002-05-24 2008-12-24 Nps Allelix Corp Verfahren zur enzymatischen herstellung von glp-2-(1-33)- und glp-2-(1-34)-peptiden
WO2004015112A1 (de) * 2002-07-24 2004-02-19 Fresenius Kabi Deutschland Gmbh Dns-konstrukte für die herstellung von peptiden
AU2003301559A1 (en) * 2002-10-22 2004-05-13 Board Of Regents The University Of Texas System Colostrinin and peptides thereof as modulators of intracellular signaling molecules and inhibitors of apoptosis
US8119854B2 (en) 2002-12-10 2012-02-21 Pietro P. Sanna Role of proteoglycans in drug dependence
US20050026826A1 (en) * 2003-01-17 2005-02-03 Margarethe Hoenig Feline proinsulin, insulin and constituent peptides
US20050118678A1 (en) * 2003-10-17 2005-06-02 Regents Of The University Of Minnesota Modified polypeptides with therapeutic activity and methods of use
SI1704234T1 (sl) * 2003-11-21 2012-08-31 Nps Pharma Inc Proizvodnja glukagonu podobnih peptidov 2 in analogov
CA2548749A1 (en) * 2003-12-09 2005-06-23 Ventria Bioscience High-level expression of fusion polypeptides in plant seeds utilizing seed-storage proteins as fusion carriers
WO2005072766A2 (en) * 2004-01-27 2005-08-11 Boys Town National Research Hospital Peptides that bind to hsp90 proteins
ES2533002T3 (es) * 2004-03-10 2015-04-06 Creighton University Receptores estrogénicos y métodos de uso
SE0401665D0 (sv) * 2004-06-24 2004-06-24 Amersham Biosciences Ab Purification of immunoglobulins
US7910101B2 (en) 2004-10-25 2011-03-22 Centocor, Inc. Melanocortin receptor binding mimetibodies, compositions, methods and uses
EP2069386A4 (de) 2006-07-21 2009-10-28 Life Technologies Corp Proteinmolekülgewichtsstandards mit hoher trennschärfe
US20110045557A1 (en) * 2007-07-27 2011-02-24 Mohammed Ataai Novel Fusion Carbonic Anhydrase/Cellulose Binding Polypeptide Encoded by a Novel Hybrid Gene, and Method of Creating and Using the Same
US9737480B2 (en) 2012-02-06 2017-08-22 President And Fellows Of Harvard College ARRDC1-mediated microvesicles (ARMMs) and uses thereof
US9816080B2 (en) 2014-10-31 2017-11-14 President And Fellows Of Harvard College Delivery of CAS9 via ARRDC1-mediated microvesicles (ARMMs)
EP3518981A4 (de) 2016-10-03 2020-06-10 President and Fellows of Harvard College Abgabe von therapeutischen rnas über arrdc1-vermittelte mikrovesikel
EP4034088A4 (de) 2019-09-26 2023-10-11 President and Fellows of Harvard College Protein-1- (arrdc1)-konstrukte enthaltende minimale arrestin-domäne

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
IL60184A (en) * 1979-05-31 1984-05-31 Schering Ag Process for the specific cleavage of protein sequences from proteins
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
US4880911A (en) * 1982-03-19 1989-11-14 G. D. Searle & Co. Fused polypeptides and methods for their detection
US4728609A (en) * 1983-01-10 1988-03-01 Hoffmann-La-Roche Inc. Recombinant growth hormone releasing factor
GB8308235D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
GB8314362D0 (en) * 1983-05-24 1983-06-29 Celltech Ltd Polypeptide and protein products
US4745051A (en) * 1983-05-27 1988-05-17 The Texas A&M University System Method for producing a recombinant baculovirus expression vector
US4782137A (en) * 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4784949A (en) * 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US5155214A (en) * 1984-03-05 1992-10-13 The Salk Institute For Biological Studies Basic fibroblast growth factor
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
US4761375A (en) * 1984-05-08 1988-08-02 Genetics Institute, Inc. Human interleukin-2 cDNA sequence
GB2162190A (en) * 1984-07-06 1986-01-29 Pa Consulting Services Improvements in or relating to production of silk
JP2624470B2 (ja) * 1984-10-02 1997-06-25 バイオジェン インコーポレイテッド ストレプトアビジン様ポリペプチドの製造
DE3523701A1 (de) * 1985-07-03 1987-01-08 Bayer Ag Verfahren zur herstellung von proteinen und polypeptiden
GB8518753D0 (en) * 1985-07-24 1985-08-29 Glaxo Group Ltd Microbiological products
US4801536A (en) * 1985-10-11 1989-01-31 Genetics Institute, Inc. Method for producing heterologous proteins
SE8505922D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering
US4868113A (en) * 1986-03-03 1989-09-19 Rorer Biotechnology, Inc. Recombinant DNA vector encoding human endothelial cell growth factor
EP0244147A3 (de) * 1986-04-28 1989-04-05 Merck & Co. Inc. Verfahren zur Reinigung hybrider Proteine
JPS62283998A (ja) * 1986-06-02 1987-12-09 Otsuka Pharmaceut Co Ltd 蛋白質抗体の製造法
US5340725A (en) * 1986-10-09 1994-08-23 Fujisawa Pharmaceutical Co., Ltd. Expression vector for insulin-like growth factor I and method of production
EP0286239B1 (de) * 1987-03-10 1996-03-13 New England Biolabs, Inc. Herstellung und Reinigung eines Proteins, das mit einem Bindungsprotein fusioniert ist
US5013653A (en) * 1987-03-20 1991-05-07 Creative Biomolecules, Inc. Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage
WO1988007085A1 (en) * 1987-03-20 1988-09-22 Creative Biomolecules, Inc. Process for the purification of recombinant polypeptides
PT87133B (pt) * 1987-04-02 1992-07-31 Amrad Corp Ltd Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif
SE501169C2 (sv) * 1988-02-05 1994-11-28 Aake Nygren Framställning av serumalbuminbindande fusionsproteiner vilka innehåller protein G-domäner
US4931498A (en) * 1988-02-25 1990-06-05 Purdue Research Foundation Immobilized artificial membranes
SE8800981D0 (sv) * 1988-03-17 1988-03-17 Nygren Per Ake A recombinant fusion protein, its use and a recombinant vector
US5137819A (en) * 1988-07-08 1992-08-11 University Of British Columbia Cellulose binding fusion proteins for immobilization and purification of polypeptides
US5082774A (en) * 1988-08-30 1992-01-21 The General Hospital Corporation Recombinant human nerve growth factor
US5102789A (en) * 1989-03-15 1992-04-07 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of epideramal growth factor in pichia pastoris yeast cells
US5599907A (en) * 1989-05-10 1997-02-04 Somatogen, Inc. Production and use of multimeric hemoglobins
US5053133A (en) * 1990-02-09 1991-10-01 Elias Klein Affinity separation with activated polyamide microporous membranes
US5574138A (en) * 1993-03-08 1996-11-12 Immunex Corporation Epithelium-derived T-cell factor

Also Published As

Publication number Publication date
ZA915307B (en) 1993-02-24
US6127150A (en) 2000-10-03
AU8915591A (en) 1992-02-18
EP0539530A1 (de) 1993-05-05
NZ238812A (en) 1993-01-27
IE912464A1 (en) 1992-01-29
WO1992001707A1 (en) 1992-02-06
EP0539530B1 (de) 2000-02-02
AU662302B2 (en) 1995-08-31
JP3129437B2 (ja) 2001-01-29
CA2087261A1 (en) 1992-01-17
US5595887A (en) 1997-01-21
CA2087261C (en) 2002-12-24
EP0539530A4 (en) 1993-09-15
DK0539530T3 (da) 2000-06-26
JPH06505624A (ja) 1994-06-30
DE69131961D1 (de) 2000-03-09

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