ATE193330T1 - Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen - Google Patents
Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymenInfo
- Publication number
- ATE193330T1 ATE193330T1 AT94914164T AT94914164T ATE193330T1 AT E193330 T1 ATE193330 T1 AT E193330T1 AT 94914164 T AT94914164 T AT 94914164T AT 94914164 T AT94914164 T AT 94914164T AT E193330 T1 ATE193330 T1 AT E193330T1
- Authority
- AT
- Austria
- Prior art keywords
- sample
- enzymatically active
- solid support
- assaying
- indicator
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 9
- 108090000790 Enzymes Proteins 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 5
- 230000003301 hydrolyzing effect Effects 0.000 title abstract 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 title 1
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005804 Peptidases Proteins 0.000 title 1
- 239000004365 Protease Substances 0.000 title 1
- 229940009098 aspartate Drugs 0.000 title 1
- 230000000694 effects Effects 0.000 title 1
- 102000004157 Hydrolases Human genes 0.000 abstract 7
- 108090000604 Hydrolases Proteins 0.000 abstract 7
- 239000007787 solid Substances 0.000 abstract 6
- 238000012360 testing method Methods 0.000 abstract 4
- 102000035101 Aspartic proteases Human genes 0.000 abstract 3
- 108091005502 Aspartic proteases Proteins 0.000 abstract 3
- 241000222122 Candida albicans Species 0.000 abstract 3
- 206010007134 Candida infections Diseases 0.000 abstract 3
- 201000003984 candidiasis Diseases 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 239000013626 chemical specie Substances 0.000 abstract 1
- 201000010099 disease Diseases 0.000 abstract 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 1
- 239000013642 negative control Substances 0.000 abstract 1
- 244000052769 pathogen Species 0.000 abstract 1
- 230000001717 pathogenic effect Effects 0.000 abstract 1
- 239000013641 positive control Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4731—Casein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/805—Haemoglobins; Myoglobins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/048,536 US5416003A (en) | 1993-04-14 | 1993-04-14 | Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities |
| PCT/US1994/004098 WO1994024306A1 (en) | 1993-04-14 | 1994-04-13 | Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities |
| CN94192191A CN1096501C (zh) | 1993-04-14 | 1994-04-13 | 报告酶释放技术∶分析天冬氨酸蛋白酶及其它水解酶活性是否存在的方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE193330T1 true ATE193330T1 (de) | 2000-06-15 |
Family
ID=37698030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT94914164T ATE193330T1 (de) | 1993-04-14 | 1994-04-13 | Reporterenzym-freisetzungstechnologie: verfahren zum nachweis der gegenwart von aspartat-proteasen und anderen aktivitaeten von hydrolytischen enzymen |
Country Status (6)
| Country | Link |
|---|---|
| US (3) | US5416003A (de) |
| EP (1) | EP0694077B1 (de) |
| CN (1) | CN1096501C (de) |
| AT (1) | ATE193330T1 (de) |
| DE (1) | DE69424663T2 (de) |
| RU (1) | RU2159818C2 (de) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU721069B2 (en) * | 1993-04-14 | 2000-06-22 | Litmus Concepts, Inc. | Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities |
| US5416003A (en) * | 1993-04-14 | 1995-05-16 | Litmus Concepts, Inc. | Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities |
| US5571684A (en) * | 1994-11-07 | 1996-11-05 | Litmus Concepts, Inc. | Assay for proline iminopeptidase and other hydrolytic activities |
| EP0790861A1 (de) * | 1995-09-12 | 1997-08-27 | Becton, Dickinson and Company | Verfahren und vorrichtung zur dns-amplifikation und test |
| US20020086399A1 (en) * | 1996-02-29 | 2002-07-04 | Fuji Photo Film Co., Ltd. | Method of measurement of protease and thin membranes used for said method |
| EP0912757A4 (de) * | 1996-05-14 | 2003-03-05 | Univ Manitoba | Bestimmung biologisch aktiver substanzen mit hilfe von festphasen |
| CA2189486A1 (en) * | 1996-11-04 | 1998-05-04 | Yves St-Pierre | Analysis of enzyme activity using immobilized fluorescence-labeled substrates |
| US6042878A (en) | 1996-12-31 | 2000-03-28 | General Electric Company | Method for depositing a ceramic coating |
| US6451607B1 (en) | 1998-05-07 | 2002-09-17 | Litmus Concepts, Inc. | External dried-reagent control for analytical test devices |
| US7700305B2 (en) | 1999-09-17 | 2010-04-20 | N2Itive1 Innovations | Analyte detection |
| US7166475B2 (en) * | 1999-02-26 | 2007-01-23 | Cyclacel Ltd. | Compositions and methods for monitoring the modification state of a pair of polypeptides |
| US6426189B1 (en) | 1999-10-01 | 2002-07-30 | Novozymes A/S | Cellulose films for screening |
| DE60016951T2 (de) * | 1999-10-01 | 2005-12-15 | Novozymes A/S | Zellulosefilme zur durchfuehrung von screening-verfahren |
| US6627396B1 (en) * | 1999-10-28 | 2003-09-30 | The Regents Of The University Of California | Influenza sensor |
| US8182986B1 (en) * | 2001-07-12 | 2012-05-22 | Discoverx | Assays for nucleic acid modifying enzymes |
| US6942972B2 (en) | 2001-10-24 | 2005-09-13 | Beckman Coulter, Inc. | Efficient synthesis of protein-oligonucleotide conjugates |
| JP4496407B2 (ja) * | 2002-05-13 | 2010-07-07 | ベクトン・ディキンソン・アンド・カンパニー | プロテアーゼ阻害剤試料採取システム |
| EP1549224A1 (de) * | 2002-10-10 | 2005-07-06 | Becton Dickinson and Company | Probeentnahmesystem mit caspase-inhibitor |
| US7041469B2 (en) * | 2002-10-10 | 2006-05-09 | Quidel Corporation | Assays for trichomonal and other hydrolases |
| EP1656443A4 (de) * | 2003-05-02 | 2007-06-06 | Sigma Aldrich Co | Festphasen-zellyse und einfangplattform |
| US20050124965A1 (en) * | 2003-12-08 | 2005-06-09 | Becton, Dickinson And Company | Phosphatase inhibitor sample collection system |
| GB2422664A (en) * | 2005-01-28 | 2006-08-02 | Ethicon Inc | Device for detecting an enzyme in a sample |
| US20050191704A1 (en) * | 2004-03-01 | 2005-09-01 | Kimberly-Clark Worldwide, Inc. | Assay devices utilizing chemichromic dyes |
| US7521226B2 (en) | 2004-06-30 | 2009-04-21 | Kimberly-Clark Worldwide, Inc. | One-step enzymatic and amine detection technique |
| US7094528B2 (en) | 2004-06-30 | 2006-08-22 | Kimberly-Clark Worldwide, Inc. | Magnetic enzyme detection techniques |
| US7906276B2 (en) * | 2004-06-30 | 2011-03-15 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
| WO2006026248A1 (en) * | 2004-08-25 | 2006-03-09 | Sigma-Aldrich Co. | Compositions and methods employing zwitterionic detergent combinations |
| US7514230B2 (en) * | 2004-10-22 | 2009-04-07 | Kimberly-Clark Worldwide, Inc. | Detection of trichomonas |
| KR100589354B1 (ko) | 2005-01-25 | 2006-06-14 | 한국과학기술원 | 내부 다당류분해효소와 내부 단백질분해효소를 생산하는미생물을 동시에 탐지 및 선별하기 위한 배지 조성 |
| US7504235B2 (en) | 2005-08-31 | 2009-03-17 | Kimberly-Clark Worldwide, Inc. | Enzyme detection technique |
| US7745158B2 (en) * | 2005-12-14 | 2010-06-29 | Kimberly-Clark Worldwide, Inc. | Detection of secreted aspartyl proteases from Candida species |
| US20070134747A1 (en) * | 2005-12-14 | 2007-06-14 | Kimberly-Clark Worldwide, Inc. | Detection of secreted lipase proteins from Candida species |
| US8758989B2 (en) * | 2006-04-06 | 2014-06-24 | Kimberly-Clark Worldwide, Inc. | Enzymatic detection techniques |
| US8163237B2 (en) * | 2006-05-08 | 2012-04-24 | Becton, Dickinson And Company | Vascular access device pathogenic status indication |
| US7897360B2 (en) | 2006-12-15 | 2011-03-01 | Kimberly-Clark Worldwide, Inc. | Enzyme detection techniques |
| ES2453379T3 (es) * | 2007-04-25 | 2014-04-07 | 3M Innovative Properties Company | Reactivos soportados, métodos y dispositivos |
| EP2380990A1 (de) * | 2010-04-20 | 2011-10-26 | Asociación Centro de Investigación Cooperativa en Biomateriales - CIC biomaGUNE | Analytische Anwendungen von enzymatischem Wachstum für fluoreszierende Quantenpunkte |
| GB201021509D0 (en) * | 2010-12-20 | 2011-02-02 | Reactivlab Ltd | Assay method |
| US8956518B2 (en) | 2011-04-20 | 2015-02-17 | Lifescan, Inc. | Electrochemical sensors with carrier field |
| EP2745114B1 (de) | 2011-09-16 | 2018-10-17 | Credo Biomedical Pte Ltd. | Molekulare diagnostische testvorrichtung und verwendungsverfahren |
| KR101698667B1 (ko) * | 2015-04-03 | 2017-01-20 | 아주대학교산학협력단 | 변색 센서 및 이의 제조 방법 |
| US10563164B1 (en) | 2015-10-08 | 2020-02-18 | Charm Sciences, Inc. | Plate reader |
| WO2017139679A1 (en) | 2016-02-12 | 2017-08-17 | The Regents Of The University Of California | Systems and compositions for diagnosing pathogenic fungal infection and methods of using the same |
| US10495563B1 (en) | 2016-04-28 | 2019-12-03 | Charm Sciences, Inc. | Plate reader observation methods and operation |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1320086A (en) * | 1970-09-11 | 1973-06-13 | Boehringer Mannheim Gmbh | Agent and method for the determination of hydrolysing enzymes |
| CA1190223A (en) * | 1977-11-01 | 1985-07-09 | National Research Development Corporation | Substrates for enzymes |
| US4859581A (en) * | 1986-03-10 | 1989-08-22 | Board Of Regents, The University Of Texas System | Endoglycosidase assay |
| US4789629A (en) * | 1986-11-24 | 1988-12-06 | Smithkline Beckman Corporation | Method and device for collecting and testing for fecal occult blood |
| US5202233A (en) * | 1988-07-11 | 1993-04-13 | Boehringer Mannheim Gmbh | Process for the detection of substances with hydrolase activity |
| US5156954A (en) * | 1989-03-08 | 1992-10-20 | Cholestech Corporation | Assay device and method using a signal-modulating compound |
| US5132085A (en) * | 1991-02-28 | 1992-07-21 | Eastman Kodak Company | Test device with novel control symbolism |
| US5120718A (en) * | 1991-06-13 | 1992-06-09 | Abbott Laboratories | Candida acid protease inhibiting compounds |
| US5288612A (en) * | 1991-07-03 | 1994-02-22 | The Scripps Research Institute | Assay methods for detecting serum proteases, particularly activated protein C |
| US5268146A (en) * | 1992-03-25 | 1993-12-07 | Litmus Concepts, Inc. | Fast response test panel |
| DE4217474A1 (de) * | 1992-05-27 | 1993-12-02 | Boehringer Mannheim Gmbh | Verfahren und Mittel zur Bestimmung eines Analyts |
| US5416003A (en) * | 1993-04-14 | 1995-05-16 | Litmus Concepts, Inc. | Reporter enzyme release technology: methods of assaying for the presence of aspartic proteases and other hydrolytic enzyme activities |
-
1993
- 1993-04-14 US US08/048,536 patent/US5416003A/en not_active Expired - Lifetime
-
1994
- 1994-04-13 RU RU95122627/13A patent/RU2159818C2/ru not_active IP Right Cessation
- 1994-04-13 AT AT94914164T patent/ATE193330T1/de not_active IP Right Cessation
- 1994-04-13 EP EP94914164A patent/EP0694077B1/de not_active Expired - Lifetime
- 1994-04-13 CN CN94192191A patent/CN1096501C/zh not_active Expired - Fee Related
- 1994-04-13 DE DE69424663T patent/DE69424663T2/de not_active Expired - Fee Related
- 1994-11-30 US US08/347,763 patent/US5585273A/en not_active Expired - Lifetime
-
1995
- 1995-03-17 US US08/406,239 patent/US6251621B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0694077A1 (de) | 1996-01-31 |
| US5416003A (en) | 1995-05-16 |
| DE69424663D1 (de) | 2000-06-29 |
| CN1138877A (zh) | 1996-12-25 |
| US6251621B1 (en) | 2001-06-26 |
| RU2159818C2 (ru) | 2000-11-27 |
| CN1096501C (zh) | 2002-12-18 |
| US5585273A (en) | 1996-12-17 |
| DE69424663T2 (de) | 2000-09-28 |
| EP0694077B1 (de) | 2000-05-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |