ATE194355T1 - Verfahren zur synthese von oligonukleotiden - Google Patents

Verfahren zur synthese von oligonukleotiden

Info

Publication number: ATE194355T1
Authority: AT; Austria
Prior art keywords: segments; solution; alcohol group; linkage; residual
Prior art date: 1995-06-07

Application number

AT96915865T

Other languages

English (en)

Inventor

Douglas Alan Livingston

Original Assignee

Jolla Pharma

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-06-07

Filing date

1996-05-16

Publication date

2000-07-15

1996-05-16 Application filed by Jolla Pharma filed Critical Jolla Pharma

2000-07-15 Application granted granted Critical

2000-07-15 Publication of ATE194355T1 publication Critical patent/ATE194355T1/de

Links

108091034117 Oligonucleotide Proteins 0.000 title abstract 3
238000000034 method Methods 0.000 title abstract 3
230000015572 biosynthetic process Effects 0.000 title abstract 2
238000003786 synthesis reaction Methods 0.000 title abstract 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
125000000075 primary alcohol group Chemical group 0.000 abstract 3
125000003198 secondary alcohol group Chemical group 0.000 abstract 3
125000000524 functional group Chemical group 0.000 abstract 2
239000002777 nucleoside Substances 0.000 abstract 2
125000003835 nucleoside group Chemical group 0.000 abstract 2
239000012071 phase Substances 0.000 abstract 2
239000003153 chemical reaction reagent Substances 0.000 abstract 1
230000008878 coupling Effects 0.000 abstract 1
238000010168 coupling process Methods 0.000 abstract 1
238000005859 coupling reaction Methods 0.000 abstract 1
239000000539 dimer Substances 0.000 abstract 1
239000000203 mixture Substances 0.000 abstract 1
238000002515 oligonucleotide synthesis Methods 0.000 abstract 1
SXADIBFZNXBEGI-UHFFFAOYSA-N phosphoramidous acid Chemical group NP(O)O SXADIBFZNXBEGI-UHFFFAOYSA-N 0.000 abstract 1
239000007790 solid phase Substances 0.000 abstract 1
239000013638 trimer Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Biotechnology (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Saccharide Compounds (AREA)

AT96915865T 1995-06-07 1996-05-16 Verfahren zur synthese von oligonukleotiden ATE194355T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US08/474,999 US5856464A (en)	1995-06-07	1995-06-07	Selective capping solution phase oligonucleotide synthesis
PCT/US1996/007100 WO1996040708A2 (en)	1995-06-07	1996-05-16	Improved methods for oligonucleotide synthesis

Publications (1)

Publication Number	Publication Date
ATE194355T1 true ATE194355T1 (de)	2000-07-15

Family

ID=23885823

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT96915865T ATE194355T1 (de)	1995-06-07	1996-05-16	Verfahren zur synthese von oligonukleotiden

Country Status (10)

Country	Link
US (2)	US5856464A (de)
EP (1)	EP0830366B1 (de)
JP (1)	JPH11510790A (de)
KR (1)	KR19990022711A (de)
CN (1)	CN1190973A (de)
AT (1)	ATE194355T1 (de)
AU (1)	AU694359B2 (de)
CA (1)	CA2222440A1 (de)
DE (1)	DE69609184T2 (de)
WO (1)	WO1996040708A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5856464A (en) *	1995-06-07	1999-01-05	Lajolla Pharmaceutical Company	Selective capping solution phase oligonucleotide synthesis
WO1999008110A1 (en)	1997-08-08	1999-02-18	Newbiotics, Inc.	Methods and compositions for overcoming resistance to biologic and chemotherapy
IL137164A0 (en) *	1998-01-23	2001-07-24	Newbiotics Inc	Enzyme catalyzed therapeutic agents
US7462605B2 (en) *	1998-01-23	2008-12-09	Celmed Oncology (Usa), Inc.	Phosphoramidate compounds and methods of use
US6096899A (en) *	1998-04-14	2000-08-01	The Regents Of The University Of Michigan	Cylic imidazole compounds having relatively low hydrogen content and relatively high nitrogen content and polymers formed therefrom
US6683061B1 (en) *	1999-07-22	2004-01-27	Newbiotics, Inc.	Enzyme catalyzed therapeutic activation
BR0012676A (pt) *	1999-07-22	2003-07-01	Newbiotics Inc	Métodos para tratamento de tumores resistentes a terapia
US6451998B1 (en)	1999-10-18	2002-09-17	Agilent Technologies, Inc.	Capping and de-capping during oligonucleotide synthesis
MXPA02005236A (es) *	1999-11-28	2004-03-19	Jolla Pharma	Metodos de tratamiento de lupus con base en la afinidad de anticuerpos y metodos de separacion por exclusion y composiciones para el uso de los mismos.
US7135565B2 (en) *	2000-07-28	2006-11-14	Agilent Technologies, Inc.	Synthesis of polynucleotides using combined oxidation/deprotection chemistry
AU2001284268A1 (en) *	2000-09-07	2002-03-22	Avecia Biotechnology, Inc.	Synthons for oligonucleotide synthesis
US7138388B2 (en)	2001-01-19	2006-11-21	Celmed Oncology (Usa), Inc.	Methods to treat autoimmune and inflammatory conditions
US20030114405A1 (en) *	2001-08-13	2003-06-19	Linnik Matthew D.	Methods of treating systemic lupus erythematosus in individuals having significantly impaired renal function
DE10142643A1 (de) *	2001-08-31	2003-04-24	Clondiag Chip Tech Gmbh	Detektion von Wechselwirkungen auf Sonden-Arrays
US7105135B2 (en) *	2001-10-16	2006-09-12	Lockheed Martin Corporation	System and method for large scale detection of hazardous materials in the mail or in other objects
US6696298B2 (en)	2001-12-07	2004-02-24	Biosearch Technologies, Inc.	Multi-channel reagent dispensing apparatus
WO2004060320A2 (en) *	2002-12-27	2004-07-22	La Jolla Pharmaceutical Company	Methods of improving health-related quality of life in individuals with systemic lupus erythematosus
US20040258683A1 (en) *	2003-03-30	2004-12-23	Linnik Matthew D.	Methods of treating and monitoring systemic lupus erythematosus in individuals
US20070136064A1 (en) *	2003-04-16	2007-06-14	Carroll David W	Mobile personal computer with movement sensor
US8501931B2 (en) *	2006-10-12	2013-08-06	Research Foundation Of The City University Of New York	Convertible nucleoside derivatives
US20090007775A1 (en) *	2007-03-07	2009-01-08	Seymour John C	Engine for Aeronautical Applications II
CN102344477B (zh) *	2010-07-27	2015-04-08	苏州瑞博生物技术有限公司	一种核苷酸和/或寡核苷酸及其制备方法
EP3426671A4 (de) *	2016-03-11	2019-11-20	Spring Bank Pharmaceuticals, Inc.	Verbindungen und zusammensetzungen zur behandlung von infektionen
WO2019158007A1 (zh) *	2018-02-13	2019-08-22	深圳华大生命科学研究院	合成寡核苷酸的方法及系统
CN112533694A (zh) *	2018-05-29	2021-03-19	生捷科技控股公司	监测固相逐步寡核苷酸合成的方法与系统
CN113993876B (zh) *	2019-06-11	2024-06-14	豪夫迈·罗氏有限公司	使用改进的氧化方案制备寡核苷酸的方法
FR3105678A1 (fr) *	2019-12-20	2021-06-25	Orange	Procédé de résolution d’identifiants de nommage
JP2024506042A (ja) *	2021-02-08	2024-02-08	ラクティゲンセラピューティクス	多価オリゴヌクレオチド薬剤およびその使用方法
KR102945259B1 (ko) *	2021-09-30	2026-03-27	주식회사 엘지화학	마이크로 캐리어, 세포 복합체 및 이를 포함하는 의료용 조성물, 미용 조성물, 의료 용품 및 미용 용품

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4415732A (en) *	1981-03-27	1983-11-15	University Patents, Inc.	Phosphoramidite compounds and processes
US5430136A (en) *	1984-10-16	1995-07-04	Chiron Corporation	Oligonucleotides having selectably cleavable and/or abasic sites
US5367066A (en) *	1984-10-16	1994-11-22	Chiron Corporation	Oligonucleotides with selectably cleavable and/or abasic sites
US4959463A (en) *	1985-10-15	1990-09-25	Genentech, Inc.	Intermediates
US5214134A (en) *	1990-09-12	1993-05-25	Sterling Winthrop Inc.	Process of linking nucleosides with a siloxane bridge
EP0678096B1 (de) *	1993-01-08	1997-03-19	HYBRIDON, Inc.	Synthese von dimer-blöcken und ihre verwendung bei der zusammensetzung von oligonukleotiden
US5856464A (en) *	1995-06-07	1999-01-05	Lajolla Pharmaceutical Company	Selective capping solution phase oligonucleotide synthesis

1995
- 1995-06-07 US US08/474,999 patent/US5856464A/en not_active Expired - Lifetime
1996
- 1996-05-16 WO PCT/US1996/007100 patent/WO1996040708A2/en not_active Ceased
- 1996-05-16 KR KR1019970709153A patent/KR19990022711A/ko not_active Withdrawn
- 1996-05-16 CN CN96195563A patent/CN1190973A/zh active Pending
- 1996-05-16 AT AT96915865T patent/ATE194355T1/de not_active IP Right Cessation
- 1996-05-16 CA CA002222440A patent/CA2222440A1/en not_active Abandoned
- 1996-05-16 AU AU57524/96A patent/AU694359B2/en not_active Ceased
- 1996-05-16 DE DE69609184T patent/DE69609184T2/de not_active Expired - Fee Related
- 1996-05-16 JP JP9500588A patent/JPH11510790A/ja not_active Ceased
- 1996-05-16 EP EP96915865A patent/EP0830366B1/de not_active Expired - Lifetime
1998
- 1998-12-16 US US09/213,991 patent/US6166198A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
JPH11510790A (ja)	1999-09-21
AU5752496A (en)	1996-12-30
EP0830366B1 (de)	2000-07-05
WO1996040708A3 (en)	1997-02-20
KR19990022711A (ko)	1999-03-25
US5856464A (en)	1999-01-05
CN1190973A (zh)	1998-08-19
WO1996040708A2 (en)	1996-12-19
AU694359B2 (en)	1998-07-16
EP0830366A2 (de)	1998-03-25
DE69609184T2 (de)	2001-02-15
DE69609184D1 (de)	2000-08-10
US6166198A (en)	2000-12-26
CA2222440A1 (en)	1996-12-19

Legal Events

Date	Code	Title	Description
2001-01-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE69609184D1 (de)	2000-08-10	Verfahren zur synthese von oligonukleotiden
Shibahara et al.	1987	Site-directed cleavage of RNA
Agarwal et al.	1970	Total synthesis of the gene for an alanine transfer ribonucleic acid from yeast
Ojala et al.	1981	tRNA punctuation model of RNA processing in human mitochondria
Ishihara et al.	1979	Complete structure of the carbohydrate moiety of stem bromelain. An application of the almond glycopeptidase for structural studies of glycopeptides.
Acevedo et al.	1987	Non-enzymatic transcription of an oligodeoxynucleotide 14 residues long
Holley et al.	1963	Chemistry of amino acid-specific ribonucleic acids
Holley	1965	Structure of an alanine transfer ribonucleic acid
Hirose et al.	1978	Rapid synthesis of trideoxyribonucleotide blocks
PT693065E (pt)	2002-05-31	Derivados de cumarina nao-nucleosidicos como agentes reticulantes de polionucleotidos
WO2001048184A3 (de)	2002-05-16	Verfahren zur parallelen sequenzierung eines nukleinsäuregemisches an einer oberfläche
CO4600741A1 (es)	1998-05-08	Procedimiento para purificar y aislar 2´-desoxi-2´,2´-di- fluoronucleosidos
Thuong et al.	1988	Solid phase synthesis of oligo-α-and oligo-β-deoxynucleotidescovalently linked to an acridine
Tanaka et al.	1987	Solid phase synthesis of oligoribonucleotides using the o-nitrobenzyl group for 2'-hydroxyl protection and H-phosphonate chemistry
DE68909280D1 (de)	1993-10-28	Hydrierung aromatischer Amine zur Herstellung ihrer entsprechenden ringhydrierten Verbindungen.
Agarwal et al.	1978	Chemical synthesis of a self-complementary octanucleotide, dG-GTTAACC by a modified triester method
Lund et al.	1979	Initiation of Escherichia coli ribosomal RNA synthesis in vivo.
Hsiung et al.	1979	Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribo-oligonucleotides fragments constituting human insulin A DNA
EP0002322A2 (de)	1979-06-13	Ein verbessertes Triesterverfahren zur Herstellung von Oligonukleotiden
Krawetz et al.	1987	Cloning of bovine P1 protamine cDNA and the evolution of vertebrate P1 protamines
DE69424504D1 (de)	2000-06-21	Verfahren zur Herstellung von Etoposid-Phosphat und Etoposid
Usman et al.	1988	Preparation of glyceronucleoside phosphoramidite synthons and their use in the solid phase synthesis of acyclic oligonucleotides
ATE182602T1 (de)	1999-08-15	Pna-synthese unter verwendung einer basenlabilen amino-schutzgruppe
Yamana et al.	1989	A simple preparation of 5′-O-dimethoxytrityl deoxyribonucleoside 3′-O-phosphorbisdiethylamidites as useful intermediates in the synthesis of oligodeoxyribonucleotides and their phosphorodiethylamidate analogs on a solid support
AU7304394A (en)	1995-03-30	TTTr as protective group in nucleotide synthesis