ATE194656T1 - METHOD FOR PRODUCING A FOREIGN GENE OR ITS PRODUCT IN PLANT CELLS - Google Patents

METHOD FOR PRODUCING A FOREIGN GENE OR ITS PRODUCT IN PLANT CELLS

Info

Publication number
ATE194656T1
ATE194656T1 AT93106894T AT93106894T ATE194656T1 AT E194656 T1 ATE194656 T1 AT E194656T1 AT 93106894 T AT93106894 T AT 93106894T AT 93106894 T AT93106894 T AT 93106894T AT E194656 T1 ATE194656 T1 AT E194656T1
Authority
AT
Austria
Prior art keywords
cdna
plant
gene
product
rna
Prior art date
Application number
AT93106894T
Other languages
German (de)
Inventor
Masashi Mori
Tetsuro Okuno
Iwao Furusawa
Original Assignee
Nihon Nohyaku Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nihon Nohyaku Co Ltd filed Critical Nihon Nohyaku Co Ltd
Application granted granted Critical
Publication of ATE194656T1 publication Critical patent/ATE194656T1/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/127RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/555Interferons [IFN]
    • C07K14/57IFN-gamma
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203Virus mediated transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A process for production of an exogenous gene or its product in a plant cell which comprises: inserting into a genome of a plant; a) cDNA of replicase gene from RNA plant virus, and b) cDNA of a recombinant virus genomic RNA in which nucleotide moiety at and after ATG downstream from the original translation initiation codon (the first ATG counted from the 5'-end) in the cDNA of coat protein gene is replaced with a desired exogenous gene; or inoculating a plant cell including cDNA of replicase gene of a plant virus with synthesized RNA synthesized from the cDNA of recombinant virus genomic RNA.
AT93106894T 1992-04-28 1993-04-28 METHOD FOR PRODUCING A FOREIGN GENE OR ITS PRODUCT IN PLANT CELLS ATE194656T1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15259392 1992-04-28

Publications (1)

Publication Number Publication Date
ATE194656T1 true ATE194656T1 (en) 2000-07-15

Family

ID=15543832

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93106894T ATE194656T1 (en) 1992-04-28 1993-04-28 METHOD FOR PRODUCING A FOREIGN GENE OR ITS PRODUCT IN PLANT CELLS

Country Status (6)

Country Link
US (1) US5418153A (en)
EP (1) EP0573767B1 (en)
AT (1) ATE194656T1 (en)
AU (1) AU668387B2 (en)
CA (1) CA2095109C (en)
DE (1) DE69328994T2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5977438A (en) * 1988-02-26 1999-11-02 Biosource Technologies, Inc. Production of peptides in plants as viral coat protein fusions
US6660500B2 (en) 1988-02-26 2003-12-09 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusions
US7033835B1 (en) 1988-02-26 2006-04-25 Large Scale Biology Corporation Production of peptides in plants as viral coat protein fusions
JPH04121200A (en) * 1990-09-07 1992-04-22 Nippon Nohyaku Co Ltd Production of polypeptide in plant cell
IL108241A (en) * 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
EP0677113B1 (en) * 1992-12-30 2003-03-12 Biosource Genetics Corporation Viral amplification of recombinant messenger rna in transgenic plants
DE4315109A1 (en) * 1993-05-06 1994-11-10 Inst Pflanzengenetik & Kultur Method and vector construct for increasing expression of transgenes
GB9311593D0 (en) * 1993-06-04 1993-07-21 Sandoz Ltd Improvements in or relating to organic compounds
GB9401780D0 (en) * 1994-01-31 1994-03-23 Nickerson Biocem Ltd Modified plants
FI110323B (en) 1997-06-02 2002-12-31 Timo Korpela A recombinant construct to increase gene expression in plants
US20030074677A1 (en) * 1998-05-22 2003-04-17 Lada Rasochova Improved materials and methods for transformation
GB2364705A (en) * 2000-07-14 2002-02-06 Icgeb Fusion protein expression in plastids
GB0213298D0 (en) * 2002-06-11 2002-07-24 Phytovation Bv Improvements in or relating to protein production
EP2118274A4 (en) * 2007-01-29 2010-09-01 Univ Ohio State Res Found SYSTEM FROM A VIRAL EXPRESSION VECTOR USED TO EXPRESS GENES IN PLANTS
EP2178365B1 (en) * 2007-07-18 2011-05-18 Dow AgroSciences LLC Rescue of plant cell cultures and suspensions after cryopreservation-induced damage

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
DE3850683T2 (en) * 1987-02-09 1994-10-27 Lubrizol Genetics Inc Hybrid RNA virus.
JPH04121200A (en) * 1990-09-07 1992-04-22 Nippon Nohyaku Co Ltd Production of polypeptide in plant cell

Also Published As

Publication number Publication date
CA2095109C (en) 2003-10-28
EP0573767B1 (en) 2000-07-12
AU668387B2 (en) 1996-05-02
DE69328994T2 (en) 2001-03-01
EP0573767A1 (en) 1993-12-15
AU3824893A (en) 1993-11-04
US5418153A (en) 1995-05-23
CA2095109A1 (en) 1993-10-29
DE69328994D1 (en) 2000-08-17

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