ATE199100T1 - Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat - Google Patents

Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat

Info

Publication number
ATE199100T1
ATE199100T1 AT97947422T AT97947422T ATE199100T1 AT E199100 T1 ATE199100 T1 AT E199100T1 AT 97947422 T AT97947422 T AT 97947422T AT 97947422 T AT97947422 T AT 97947422T AT E199100 T1 ATE199100 T1 AT E199100T1
Authority
AT
Austria
Prior art keywords
triphosphate
desoxyuridine
nucleic acid
sequencing method
acid sequencing
Prior art date
Application number
AT97947422T
Other languages
English (en)
Inventor
Linda G Lee
Barnett B Rosenblum
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE199100T1 publication Critical patent/ATE199100T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT97947422T 1996-12-05 1997-11-12 Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat ATE199100T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US3260896P 1996-12-05 1996-12-05
US08/785,581 US6017702A (en) 1996-12-05 1997-01-21 Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
PCT/US1997/020443 WO1998024930A1 (en) 1996-12-05 1997-11-12 Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate

Publications (1)

Publication Number Publication Date
ATE199100T1 true ATE199100T1 (de) 2001-02-15

Family

ID=26708657

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97947422T ATE199100T1 (de) 1996-12-05 1997-11-12 Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat

Country Status (8)

Country Link
US (1) US6017702A (de)
EP (1) EP0889973B1 (de)
JP (1) JP2999267B2 (de)
AT (1) ATE199100T1 (de)
AU (1) AU706382B2 (de)
CA (1) CA2238297C (de)
DE (1) DE69704055T2 (de)
WO (1) WO1998024930A1 (de)

Families Citing this family (73)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6413718B1 (en) 1996-05-01 2002-07-02 Visible Genetics Inc. Method for sequencing of nucleic acid polymers
US6054568A (en) * 1998-01-16 2000-04-25 The Perkin-Elmer Corporation Nucleobase oligomers
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US20050287592A1 (en) * 2000-08-29 2005-12-29 Yeda Research And Development Co. Ltd. Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks
US7668658B2 (en) * 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
GB0021286D0 (en) * 2000-08-30 2000-10-18 Gemini Genomics Ab Identification of drug metabolic capacity
US6448407B1 (en) * 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
JP2004523243A (ja) * 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
AU2002364945A1 (en) * 2001-10-25 2003-07-09 Neurogenetics, Inc. Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases
US20030224380A1 (en) * 2001-10-25 2003-12-04 The General Hospital Corporation Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases
US20030170678A1 (en) * 2001-10-25 2003-09-11 Neurogenetics, Inc. Genetic markers for Alzheimer's disease and methods using the same
WO2003040395A2 (en) * 2001-11-07 2003-05-15 Applera Corporation Universal nucleotides for nucleic acid analysis
US7222059B2 (en) 2001-11-15 2007-05-22 Siemens Medical Solutions Diagnostics Electrophoretic trace simulator
WO2003093296A2 (en) * 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
US20030220844A1 (en) * 2002-05-24 2003-11-27 Marnellos Georgios E. Method and system for purchasing genetic data
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
US20070141570A1 (en) * 2003-03-07 2007-06-21 Sequenom, Inc. Association of polymorphic kinase anchor proteins with cardiac phenotypes and related methods
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7635562B2 (en) * 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20080199480A1 (en) * 2004-07-22 2008-08-21 Sequenom, Inc. Methods for Identifying Risk of Type II Diabetes and Treatments Thereof
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060223066A1 (en) * 2005-03-29 2006-10-05 Applera Corporation Methods for normalizing and for identifying small nucleic acids
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
EP3617321B1 (de) 2006-05-31 2024-10-23 Sequenom, Inc. Kit zur nukleinsäureextraktion und -verstärkung aus einer probe
CA2654165A1 (en) 2006-06-05 2007-12-13 Cancer Care Ontario Assessment of risk for colorectal cancer
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
AU2008213634B2 (en) * 2007-02-08 2013-09-05 Sequenom, Inc. Nucleic acid-based tests for RhD typing, gender determination and nucleic acid quantification
EP2243834A1 (de) 2007-03-05 2010-10-27 Cancer Care Ontario Abschätzung des Kolorektalkarzinomrisikos
US8652780B2 (en) 2007-03-26 2014-02-18 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US7767441B2 (en) * 2007-10-25 2010-08-03 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
US7811810B2 (en) 2007-10-25 2010-10-12 Industrial Technology Research Institute Bioassay system including optical detection apparatuses, and method for detecting biomolecules
EP2271772B1 (de) 2008-03-11 2014-07-16 Sequenom, Inc. Tests auf nukleinsäurebasis zur pränatalen geschlechtsbestimmung
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP3722810A3 (de) 2009-02-11 2021-01-13 Caris MPI, Inc. Molekulare profilierung von tumoren
CA2755615A1 (en) * 2009-03-18 2010-09-23 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
EP2494077A4 (de) 2009-10-27 2013-08-21 Caris Mpi Inc Molekulare profilierung für personalisierte medizin
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
KR101545848B1 (ko) 2012-04-09 2015-08-21 (주)바이오니아 핵산중합효소로 핵산을 검출하는데 사용되는 고민감도 핵산준비방법
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
EP3888021B1 (de) 2018-11-30 2024-02-21 Caris MPI, Inc. Molekulare profilierung der nächsten generation
CA3163319A1 (en) 2019-12-02 2021-06-10 Caris Mpi, Inc. Pan-cancer platinum response predictor

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5418149A (en) * 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5035996A (en) * 1989-06-01 1991-07-30 Life Technologies, Inc. Process for controlling contamination of nucleic acid amplification reactions
US5187085A (en) * 1990-09-28 1993-02-16 Applied Biosystems, Inc. Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates)
YU187991A (sh) * 1990-12-11 1994-09-09 Hoechst Aktiengesellschaft 3-(2)-amino-ali tiol-modifikovani, s fluorescentnom bojom vezani nukleozidi, nukleotidi i oligonukleotidi, postupak za njihovo dobijanje i njihova upotreba
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AU693023B2 (en) * 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay

Also Published As

Publication number Publication date
AU706382B2 (en) 1999-06-17
CA2238297A1 (en) 1998-06-11
EP0889973B1 (de) 2001-02-07
DE69704055D1 (de) 2001-03-15
EP0889973A1 (de) 1999-01-13
US6017702A (en) 2000-01-25
JP2999267B2 (ja) 2000-01-17
JPH11506343A (ja) 1999-06-08
DE69704055T2 (de) 2001-05-23
CA2238297C (en) 2004-05-25
AU5250798A (en) 1998-06-29
WO1998024930A1 (en) 1998-06-11

Similar Documents

Publication Publication Date Title
ATE199100T1 (de) Kettenabbrechende nukleinsäure-sequenziermethode mit 2'-desoxyuridin-5'-triphosphat
IS5360A (is) Greining á kjarnsýruröð
NO862918L (no) Analyse for nukleinsyresekvenser, spesielt genetiske lesjoner.
FI881530A7 (fi) Nukleiinihappojen kokonaismutageneesimenetelmä.
DE3787771D1 (de) Elektrochemische Analysenmethode.
EP0630972A3 (de) DNS-Analyse Verfahren.
DE3674577D1 (de) Verfahren zum konzentrieren von kaolinschlamm.
DE3766194D1 (de) Verfahren zur uebertragung von farbstoffen.
DE3777786D1 (de) Verfahren zum herstellen von bauart-modellen.
DE69018625D1 (de) Verfahren zum aufkonzentrieren von chlorgas.
DE3579942D1 (de) Verfahren zum loesbarmachen von interferon.
DE3771670D1 (de) Verfahren zum entschleimen von triglyceridoelen.
DE3668560D1 (de) Verfahren zum reinigen, insbesondere entschwefelung von rauchgas.
DE3667167D1 (de) Verfahren zur herstellung von 1,5-diazido-3-nitrazapentan.
DE3680454D1 (de) Verfahren zur herstellung von halogenhaltigen (2,2)-paracyclophanen.
DE3777149D1 (de) Verfahren zum vermehren von pflanzensetzlingen.
DE3771176D1 (de) Verfahren zum zusammenbauen von teilen.
DE3774687D1 (de) Verfahren zum vermehren von zwiebelpflanzen.
DE3673049D1 (de) Verfahren zur herstellung von 1,3-dialkyl-2-imidazolidinon.
DE3773121D1 (de) Verfahren zum identifizieren gewebstypischer, unloeslicher cytoskelettproteine.
NO871038D0 (no) Dna-fragment.
DE3764424D1 (de) Verfahren zum auskleiden von rohren.
DE3579818D1 (de) Verfahren zum faerben von materialbahnen.
DE3685725D1 (de) Verfahren zum bedrucken von textilien.
DE3671204D1 (de) Verfahren zum herstellen von silanen.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties