ATE211506T1 - Ein verfahren zum nachweis biologisch aktiver substanzen - Google Patents
Ein verfahren zum nachweis biologisch aktiver substanzenInfo
- Publication number
- ATE211506T1 ATE211506T1 AT96900891T AT96900891T ATE211506T1 AT E211506 T1 ATE211506 T1 AT E211506T1 AT 96900891 T AT96900891 T AT 96900891T AT 96900891 T AT96900891 T AT 96900891T AT E211506 T1 ATE211506 T1 AT E211506T1
- Authority
- AT
- Austria
- Prior art keywords
- biologically active
- cell
- fluorescence
- active substance
- gfp
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000013543 active substance Substances 0.000 title 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 abstract 6
- 102000004144 Green Fluorescent Proteins Human genes 0.000 abstract 6
- 239000005090 green fluorescent protein Substances 0.000 abstract 6
- 229940088623 biologically active substance Drugs 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000006525 intracellular process Effects 0.000 abstract 2
- 102000001253 Protein Kinase Human genes 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 108060006633 protein kinase Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Organic Chemistry (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK11095 | 1995-01-31 | ||
| DK98295 | 1995-09-07 | ||
| PCT/DK1996/000052 WO1996023898A1 (en) | 1995-01-31 | 1996-01-31 | A method of detecting biologically active substances |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE211506T1 true ATE211506T1 (de) | 2002-01-15 |
Family
ID=26063326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96900891T ATE211506T1 (de) | 1995-01-31 | 1996-01-31 | Ein verfahren zum nachweis biologisch aktiver substanzen |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0815257B1 (de) |
| AT (1) | ATE211506T1 (de) |
| AU (1) | AU4483096A (de) |
| CA (1) | CA2217700C (de) |
| DE (1) | DE69618350T2 (de) |
| DK (1) | DK0815257T3 (de) |
| ES (1) | ES2170220T3 (de) |
| WO (1) | WO1996023898A1 (de) |
Families Citing this family (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5625048A (en) | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
| US5777079A (en) * | 1994-11-10 | 1998-07-07 | The Regents Of The University Of California | Modified green fluorescent proteins |
| US6900304B2 (en) | 1996-01-31 | 2005-05-31 | The Regents Of The University Of California | Emission ratiometric indicators of phosphorylation |
| US6803188B1 (en) * | 1996-01-31 | 2004-10-12 | The Regents Of The University Of California | Tandem fluorescent protein constructs |
| US5989835A (en) | 1997-02-27 | 1999-11-23 | Cellomics, Inc. | System for cell-based screening |
| US5925558A (en) * | 1996-07-16 | 1999-07-20 | The Regents Of The University Of California | Assays for protein kinases using fluorescent protein substrates |
| AU3801997A (en) * | 1996-07-16 | 1998-02-09 | Regents Of The University Of California, The | Assays for protein kinases using fluorescent protein substrates |
| US5912137A (en) | 1996-07-16 | 1999-06-15 | The Regents Of The University Of California | Assays for protein kinases using fluorescent |
| US6593135B2 (en) | 1996-08-16 | 2003-07-15 | The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Long wavelength engineered fluorescent proteins |
| US6124128A (en) | 1996-08-16 | 2000-09-26 | The Regents Of The University Of California | Long wavelength engineered fluorescent proteins |
| DE69731960T2 (de) * | 1996-09-24 | 2005-12-15 | Cadus Pharmaceutical Corp. | Verfahren und zusammensetzungen für die identifizierung von receptor effectoren |
| CN1238807A (zh) * | 1996-11-01 | 1999-12-15 | 基因观测公司 | 稳定的瞬时基因表达 |
| US5925449A (en) * | 1996-12-26 | 1999-07-20 | Davidovits; Joseph | Method for bonding fiber reinforcement on concrete and steel structures and resultant products |
| US6670449B1 (en) | 1997-02-13 | 2003-12-30 | Memorial Sloan-Kettering Cancer Center | Hybrid molecules and their use for optically detecting changes in cellular microenvironments |
| US6727071B1 (en) | 1997-02-27 | 2004-04-27 | Cellomics, Inc. | System for cell-based screening |
| DE69839501D1 (de) | 1997-02-27 | 2008-06-26 | Cellomics Inc | System für Screening biologischer Zellen |
| US7117098B1 (en) | 1997-02-27 | 2006-10-03 | Cellomics, Inc. | Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm |
| US5998204A (en) * | 1997-03-14 | 1999-12-07 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
| US6197928B1 (en) | 1997-03-14 | 2001-03-06 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
| RU2226215C2 (ru) | 1997-04-04 | 2004-03-27 | Борд Оф Риджентс Оф Юниверсити Оф Нибраска | Молекула днк, кодирующая дикамба-разрушающую оксигеназу, и ее применение |
| WO1998045704A2 (en) | 1997-04-07 | 1998-10-15 | Bioimage A/S | A method for extracting quantitative information relating to an influence on a cellular response |
| US6046925A (en) * | 1997-04-14 | 2000-04-04 | The Regents Of The University Of California | Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins |
| US6376257B1 (en) * | 1997-04-24 | 2002-04-23 | University Of Rochester | Detection by fret changes of ligand binding by GFP fusion proteins |
| US7541151B2 (en) | 1997-06-05 | 2009-06-02 | Duke University | Single-cell biosensor for the measurement of GPCR ligands in a test sample |
| US6528271B1 (en) | 1997-06-05 | 2003-03-04 | Duke University | Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia |
| US5891646A (en) | 1997-06-05 | 1999-04-06 | Duke University | Methods of assaying receptor activity and constructs useful in such methods |
| GB2328209A (en) * | 1997-08-16 | 1999-02-17 | Stefan Andreas Oehler | Vectors which express a coloured protein for detection of integration into a cell |
| US6495664B1 (en) | 1998-07-24 | 2002-12-17 | Aurora Biosciences Corporation | Fluorescent protein sensors of post-translational modifications |
| EP1115734A4 (de) * | 1998-09-24 | 2004-07-21 | Univ Duke | Methoden zur messung von protein-protein wechselwirkungen in lebenden zellen |
| EP1121593A2 (de) * | 1998-10-15 | 2001-08-08 | BioImage A/S | Verfahren zur ableitung quantitativer informationen über einen einfluss auf eine zelluläre respons |
| US6410255B1 (en) | 1999-05-05 | 2002-06-25 | Aurora Biosciences Corporation | Optical probes and assays |
| EP1055929A1 (de) * | 1999-05-26 | 2000-11-29 | Tibotec N.V. | Mittel und Verfahren für Medizinentdeckung und phenotypische Charakterisierung von Zellen |
| US6573059B1 (en) * | 1999-06-04 | 2003-06-03 | Rmf Dictagene S.A. | Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements |
| FI107167B (fi) | 1999-06-07 | 2001-06-15 | Marko Virta | Menetelmä mikro-organismin kasvun ja kuolemisen määrittämiseksi |
| WO2001009177A2 (en) * | 1999-07-29 | 2001-02-08 | Whitehead Institute For Biomedical Research | Affinity fluorescent proteins and uses thereof |
| US6986993B1 (en) | 1999-08-05 | 2006-01-17 | Cellomics, Inc. | System for cell-based screening |
| AU2073801A (en) | 1999-12-09 | 2001-06-18 | Cellomics, Inc. | A system for cell-based screening |
| EP1626056B1 (de) | 1999-12-30 | 2012-03-28 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zur Modulierung des Hepatozytenwachstums, der Differenzierung von Plasmazellen oder der Aktivität von T-Zellen durch Modulierung der Aktivität von XBP-1 |
| JP3829252B2 (ja) | 2001-06-08 | 2006-10-04 | 独立行政法人理化学研究所 | 蛍光蛋白質 |
| EP1499885B1 (de) | 2001-08-01 | 2009-10-21 | Cellomics, Inc. | Neue fusionsproteine und molekulare bindungsassays |
| EP1551383A4 (de) | 2002-06-24 | 2007-12-05 | Paratek Pharm Innc | VERFAHRN ZUR PRûVENTION UND BEHANDLUNG VON MIKROBIELLEN INFEKTIONEN DURCH MODULIERUNG VON TRANSKRIPTIONSFAKTOREN |
| CA2445420A1 (en) | 2003-07-29 | 2005-01-29 | Invitrogen Corporation | Kinase and phosphatase assays |
| US7619059B2 (en) | 2003-07-29 | 2009-11-17 | Life Technologies Corporation | Bimolecular optical probes |
| US20080313769A9 (en) | 2006-01-12 | 2008-12-18 | Athenix Corporation | EPSP synthase domains conferring glyphosate resistance |
| AU2007223364B2 (en) | 2006-03-02 | 2014-02-13 | Athenix Corporation | Methods and compositions for improved enzyme activity in transgenic plant |
| EP2257628A4 (de) | 2008-03-04 | 2011-11-02 | Univ Washington State | Zusammensetzungen und verfahren zur differentiellen regulation der ungesättigtkeit von fettsäuren in membranlipiden und samenöl |
| CN102165489B (zh) | 2008-09-16 | 2015-11-25 | 赫斯托克斯公司 | 生物标志物表达的可再现量化 |
| CN101768213B (zh) | 2008-12-30 | 2012-05-30 | 中国科学院遗传与发育生物学研究所 | 一种与植物分蘖数目相关的蛋白及其编码基因与应用 |
| CN101817879A (zh) | 2009-02-26 | 2010-09-01 | 中国科学院遗传与发育生物学研究所 | 金属硫蛋白及其编码基因与应用 |
| US8471107B2 (en) | 2009-04-16 | 2013-06-25 | Schillinger Genetics, Inc. | Soybeans having high germination rates and ultra-low raffinose and stachyose content |
| WO2011068870A2 (en) | 2009-12-01 | 2011-06-09 | President And Fellows Of Harvard College | Modulation of nk cell antigen specific effector activity by modulation of cxcr6 (cd186) |
| WO2012051301A1 (en) | 2010-10-12 | 2012-04-19 | President And Fellows Of Harvard College | Methods for identifying modulators of triglyceride metabolism, for modulating triglyceride metabolism and for identifying subjects at risk for abnormal triglyceride metabolism |
| US9956236B2 (en) | 2011-02-07 | 2018-05-01 | Cornell University | Methods for increasing immune responses using agents that directly bind to and activate IRE-1 |
| EP2710130B1 (de) | 2011-05-19 | 2022-06-29 | Fundación Pública Andaluza Progreso Y Salud | Effizient induzierbare lentivirale tet-on-vektor system mit zwei-promotoren |
| WO2013142571A2 (en) | 2012-03-20 | 2013-09-26 | Cornell University | Assays for the identification of compounds that modulate lipid homeostasis |
| UY35035A (es) | 2012-09-14 | 2014-04-30 | Bayer Cropscience Ag | ?molécula de ácido nucleico recombinante que codifica una enzima hppd, vector, célula huésped, semilla, planta, polipéptido, producto primario, métodos y usos?. |
| EP3049521B1 (de) | 2013-09-25 | 2019-03-06 | Cornell University | Verbindungen zur induzierung von antitumorimmunität und verfahren dafür |
| CN106459986B (zh) | 2014-03-11 | 2020-06-30 | 拜耳作物科学有限合伙公司 | Hppd变体和使用方法 |
| WO2015193653A1 (en) | 2014-06-16 | 2015-12-23 | Consejo Nacional De Investigaciones Cientificas Y Tecnicas | Oxidative resistance chimeric genes and proteins, and transgenic plants including the same |
| WO2017042259A1 (en) | 2015-09-11 | 2017-03-16 | Bayer Cropscience Aktiengesellschaft | Hppd variants and methods of use |
| WO2017184727A1 (en) | 2016-04-21 | 2017-10-26 | Bayer Cropscience Lp | Tal-effector mediated herbicide tolerance |
| BR112019010476A2 (pt) | 2016-11-23 | 2019-09-10 | BASF Agricultural Solutions Seed US LLC | molécula de ácido nucleico recombinante, vetor, célula hospedeira, planta transgênica, semente transgênica, polipeptídeo recombinante, composição, método para controlar uma população de pragas, para matar pragas, para produzir um polipeptídeo, planta ou célula vegetal, método para proteger uma planta contra uma praga, para aumentar o rendimento em uma planta, uso e produto primário |
| US20190364908A1 (en) | 2016-12-22 | 2019-12-05 | BASF Agricultural Solutions Seed US LLC | Use of cry14 for the control of nematode pests |
| AR110756A1 (es) | 2017-01-18 | 2019-05-02 | Bayer Cropscience Lp | Uso de bp005 para el control de patógenos de planta |
| CN110431234B (zh) | 2017-01-18 | 2024-04-16 | 巴斯夫农业种子解决方案美国有限责任公司 | Bp005毒素基因及其使用方法 |
| US11708565B2 (en) | 2017-03-07 | 2023-07-25 | BASF Agricultural Solutions Seesi US LLC | HPPD variants and methods of use |
| BR112020008092A2 (pt) | 2017-10-24 | 2020-09-15 | BASF Agricultural Solutions Seed US LLC | método para conferir tolerância a um herbicida e planta de soja transgênica |
| BR112020008096A2 (pt) | 2017-10-24 | 2020-11-03 | Basf Se | método para conferir tolerância a um herbicida e planta de soja transgênica |
| BR112022007119A2 (pt) | 2019-10-14 | 2022-07-05 | BASF Agricultural Solutions Seed US LLC | Molécula de ácido nucleico, ácido nucleico, polipeptídeos, vetor, célula hospedeira, planta transgênica, semente transgênica, composição, métodos para controlar uma população de pragas, para matar uma praga, para produzir um polipeptídeo, para proteger uma planta e para aumentar o rendimento em uma planta, planta, uso do ácido nucleico e produto básico |
| MX2022004552A (es) | 2019-10-14 | 2022-10-10 | BASF Agricultural Solutions Seed US LLC | Nuevos genes de resistencia a insectos y métodos de uso. |
| AU2023408197A1 (en) | 2022-12-19 | 2025-06-26 | Basf Agricultural Solutions Us Llc | Insect toxin genes and methods for their use |
| JP2026522972A (ja) | 2023-07-07 | 2026-07-09 | ビーエーエスエフ アグリカルチュラル ソリューションズ ユーエス エルエルシー | 線虫有害生物を防除するためのｃｒｙ遺伝子の使用 |
| KR20260096857A (ko) | 2023-10-27 | 2026-06-24 | 바스프 아그리컬쳐럴 솔루션즈 유에스 엘엘씨 | 선충 해충의 방제를 위한 신규 유전자의 사용 |
| WO2026010930A1 (en) | 2024-07-05 | 2026-01-08 | BASF Agricultural Solutions Seed US LLC | Use of axmi277 for the control of rotylenchulus reniformis nematode pests |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4220450A (en) * | 1978-04-05 | 1980-09-02 | Syva Company | Chemically induced fluorescence immunoassay |
| CA2169298A1 (en) * | 1993-09-10 | 1995-03-16 | Martin Chalfie | Uses of green fluorescent protein |
| WO1995021191A1 (en) * | 1994-02-04 | 1995-08-10 | William Ward | Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein |
-
1996
- 1996-01-31 AT AT96900891T patent/ATE211506T1/de not_active IP Right Cessation
- 1996-01-31 ES ES96900891T patent/ES2170220T3/es not_active Expired - Lifetime
- 1996-01-31 AU AU44830/96A patent/AU4483096A/en not_active Abandoned
- 1996-01-31 EP EP96900891A patent/EP0815257B1/de not_active Expired - Lifetime
- 1996-01-31 DE DE69618350T patent/DE69618350T2/de not_active Expired - Lifetime
- 1996-01-31 CA CA002217700A patent/CA2217700C/en not_active Expired - Lifetime
- 1996-01-31 DK DK96900891T patent/DK0815257T3/da active
- 1996-01-31 WO PCT/DK1996/000052 patent/WO1996023898A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0815257B1 (de) | 2002-01-02 |
| DE69618350T2 (de) | 2002-09-19 |
| EP0815257A1 (de) | 1998-01-07 |
| WO1996023898A1 (en) | 1996-08-08 |
| AU4483096A (en) | 1996-08-21 |
| DK0815257T3 (da) | 2002-04-22 |
| DE69618350D1 (de) | 2002-02-07 |
| CA2217700A1 (en) | 1996-08-08 |
| ES2170220T3 (es) | 2002-08-01 |
| CA2217700C (en) | 2002-12-17 |
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