ATE213783T1 - Dns amplifizierung aus einzelnen zellen - Google Patents

Dns amplifizierung aus einzelnen zellen

Info

Publication number
ATE213783T1
ATE213783T1 AT99948782T AT99948782T ATE213783T1 AT E213783 T1 ATE213783 T1 AT E213783T1 AT 99948782 T AT99948782 T AT 99948782T AT 99948782 T AT99948782 T AT 99948782T AT E213783 T1 ATE213783 T1 AT E213783T1
Authority
AT
Austria
Prior art keywords
dna
amplification
analysis
individual cells
dna amplification
Prior art date
Application number
AT99948782T
Other languages
English (en)
Inventor
Christoph Klein
Oleg Schmidt-Kittler
Original Assignee
Micromet Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Micromet Ag filed Critical Micromet Ag
Application granted granted Critical
Publication of ATE213783T1 publication Critical patent/ATE213783T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT99948782T 1998-09-18 1999-09-17 Dns amplifizierung aus einzelnen zellen ATE213783T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP98117799 1998-09-18
EP98117745 1998-09-19
PCT/EP1999/006912 WO2000017390A1 (en) 1998-09-18 1999-09-17 Dna amplification of a single cell

Publications (1)

Publication Number Publication Date
ATE213783T1 true ATE213783T1 (de) 2002-03-15

Family

ID=26149650

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99948782T ATE213783T1 (de) 1998-09-18 1999-09-17 Dns amplifizierung aus einzelnen zellen

Country Status (12)

Country Link
US (1) US6673541B1 (de)
EP (1) EP1109938B1 (de)
JP (1) JP4475488B2 (de)
AT (1) ATE213783T1 (de)
AU (1) AU755499B2 (de)
CA (1) CA2343359C (de)
DE (1) DE69900949T2 (de)
DK (1) DK1109938T3 (de)
ES (1) ES2172353T3 (de)
IL (2) IL141749A0 (de)
NO (1) NO20011316L (de)
WO (1) WO2000017390A1 (de)

Families Citing this family (120)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692952B1 (en) * 1999-11-10 2004-02-17 Massachusetts Institute Of Technology Cell analysis and sorting apparatus for manipulation of cells
AU2002247014A1 (en) * 2001-01-19 2002-07-30 Genetica, Inc. Methods and reagents for amplification and manipulation of vector and target nucleic acid sequences
WO2002088388A1 (en) * 2001-04-26 2002-11-07 Ruebben Albert A method and a device for quantification of mutation loads
US8980568B2 (en) 2001-10-11 2015-03-17 Aviva Biosciences Corporation Methods and compositions for detecting non-hematopoietic cells from a blood sample
US8986944B2 (en) 2001-10-11 2015-03-24 Aviva Biosciences Corporation Methods and compositions for separating rare cells from fluid samples
JP2005535283A (ja) 2001-11-13 2005-11-24 ルビコン ゲノミクス インコーポレイテッド ランダムフラグメント化により生成されたｄｎａ分子を用いたｄｎａ増幅および配列決定
EP1476573B1 (de) 2002-01-28 2010-01-06 Ambion, Inc. Für den nachweis von nukleinsäuren kompetente biologische rohderivate
US6977162B2 (en) * 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
IL163600A0 (en) * 2002-03-01 2005-12-18 Ravgen Inc Methods for detection of genetic disorders
US7442506B2 (en) * 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20070178478A1 (en) * 2002-05-08 2007-08-02 Dhallan Ravinder S Methods for detection of genetic disorders
EP2359689B1 (de) * 2002-09-27 2015-08-26 The General Hospital Corporation Mikrofluid-Vorrichtung zur Zelltrennung, sowie Verwendung derselben
US7709047B2 (en) 2003-01-24 2010-05-04 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
US7695752B2 (en) 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
EP1604184A4 (de) * 2003-02-27 2010-10-27 Stephen A Lesko Standardisierte bewertung von therapeutischer wirksamkeit auf basis zellulärer biomarker
US20040209299A1 (en) * 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US8206913B1 (en) 2003-03-07 2012-06-26 Rubicon Genomics, Inc. Amplification and analysis of whole genome and whole transcriptome libraries generated by a DNA polymerization process
EP1604040B1 (de) 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden
US7718364B2 (en) * 2003-03-25 2010-05-18 John Wayne Cancer Institute DNA markers for management of cancer
EP1636564A1 (de) * 2003-06-13 2006-03-22 The General Hospital Corporation Mikrofluidsysteme zur abtrennung roter blutkörperchen und von blutplättchen nach grösse aus blut
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
EP2380993B1 (de) 2004-03-08 2015-12-23 Rubicon Genomics, Inc. Verfahren zur Erstellung und Erweiterung von DNA-Bibliotheken zur sensitiven Erkennung und Analyse von DNA-Methylierung
US20060105353A1 (en) * 2004-11-16 2006-05-18 Jalal Syed M Non-invasive prenatal fetal cell diagnostic method
US20070026413A1 (en) * 2005-07-29 2007-02-01 Mehmet Toner Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026414A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026415A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070026417A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US20070059680A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for cell enrichment
US20070026416A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20090181421A1 (en) * 2005-07-29 2009-07-16 Ravi Kapur Diagnosis of fetal abnormalities using nucleated red blood cells
US8921102B2 (en) * 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
WO2007018602A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Isolation of cpg islands by thermal segregation and enzymatic selection-amplification method
WO2007018601A1 (en) 2005-08-02 2007-02-15 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction
US20070059781A1 (en) * 2005-09-15 2007-03-15 Ravi Kapur System for size based separation and analysis
US20070059718A1 (en) * 2005-09-15 2007-03-15 Mehmet Toner Systems and methods for enrichment of analytes
US20070059719A1 (en) * 2005-09-15 2007-03-15 Michael Grisham Business methods for prenatal Diagnosis
US20070059774A1 (en) * 2005-09-15 2007-03-15 Michael Grisham Kits for Prenatal Testing
US20070059683A1 (en) * 2005-09-15 2007-03-15 Tom Barber Veterinary diagnostic system
US20070092899A1 (en) * 2005-10-21 2007-04-26 The Regents Of The University Of California Multiple displacement amplification with blocker DNA
ES2301342B1 (es) * 2006-03-14 2009-05-01 Oryzon Genomics, S.A. "metodo de analisis de acidos nucleicos".
EP2029779A4 (de) * 2006-06-14 2010-01-20 Living Microsystems Inc Verwendung hoch paralleler snp-genotypisierung zur fötalen diagnose
EP2589668A1 (de) 2006-06-14 2013-05-08 Verinata Health, Inc Analyse seltener Zellen mittels Probentrennung und DNA-Etiketten
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2041299A4 (de) * 2006-07-14 2010-01-13 Aviva Biosciences Corp Verfahren und zusammensetzungen für den nachweis seltener zellen aus einer biologischen probe
US20100184152A1 (en) * 2006-10-23 2010-07-22 Vladislav Sandler Target-oriented whole genome amplification of nucleic acids
US20100159506A1 (en) * 2008-07-25 2010-06-24 Cellscape Corporation Methods and systems for genetic analysis of fetal nucleated red blood cells
CA3069082C (en) 2008-09-20 2022-03-22 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
IT1391619B1 (it) 2008-11-04 2012-01-11 Silicon Biosystems Spa Metodo per l'individuazione, selezione e analisi di cellule tumorali
US8187979B2 (en) * 2009-12-23 2012-05-29 Varian Semiconductor Equipment Associates, Inc. Workpiece patterning with plasma sheath modulation
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CN102533960B (zh) * 2010-12-31 2014-04-30 深圳华大基因科技有限公司 一种单细胞基因组分析方法及试剂盒
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP4592400A3 (de) 2012-10-17 2025-10-29 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
ITTO20120962A1 (it) 2012-10-31 2014-05-01 Silicon Biosystems Spa Metodo e kit per rivelare una sequenza di dna bersaglio wild-type e/o mutata
CA2916662C (en) 2013-06-25 2022-03-08 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
EP2881739B1 (de) * 2013-12-04 2017-05-17 Menarini Silicon Biosystems S.p.A. Verfahren und Kit zur Bestimmung der Genomintegrität und/oder der Qualität einer Bibliothek von durch deterministische Restriktionsschnittstellen-Ganzgenomamplifikation erhaltene DNA-Sequenzen
CA2931140C (en) 2014-02-05 2022-08-30 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Error-free sequencing of dna
WO2016077750A1 (en) 2014-11-14 2016-05-19 Athena Diagnostics, Inc. Methods to detect a silent carrier genotype
EP3901282B1 (de) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
EP3199641B1 (de) 2016-01-27 2021-09-29 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Mittel und verfahren zur stadiumbestimmung, typisierung und behandlung einer krebserkrankung
ITUA20162640A1 (it) * 2016-04-15 2017-10-15 Menarini Silicon Biosystems Spa Metodo e kit per la generazione di librerie di dna per sequenziamento massivo parallelo
EP3431611A1 (de) 2017-07-21 2019-01-23 Menarini Silicon Biosystems S.p.A. Verbessertes verfahren und kit zur erzeugung von dna-bibliotheken für massive parallele sequenzierung
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020123316A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
IT201900013335A1 (it) 2019-07-30 2021-01-30 Menarini Silicon Biosystems Spa Metodo per analizzare la perdita di eterozigosi (loh) a seguito di amplificazione totale del genoma basata su un sito di restrizione deterministico (drs-wga)
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
IT201900024159A1 (it) 2019-12-16 2021-06-16 Menarini Silicon Biosystems Spa Metodo e kit per amplificazione totale del genoma e analisi di molecole bersaglio in un campione biologico
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
ES2982420T3 (es) 2019-12-23 2024-10-16 10X Genomics Inc Métodos para el análisis espacial mediante el uso de la ligazón con plantilla de ARN
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
EP3859012A1 (de) 2020-02-03 2021-08-04 Albert-Ludwigs-Universität Freiburg Verfahren zur amplifizierung von genomischen dna und erstellung von sequenzierungsbibliotheken
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
EP4414459B1 (de) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
EP4153776B1 (de) 2020-05-22 2025-03-05 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4729631A2 (de) 2020-12-21 2026-04-22 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
IT202100024101A1 (it) 2021-09-20 2023-03-20 Menarini Silicon Biosystems Spa Metodo per analizzare il grado di similarita' di almeno due campioni utilizzando amplificazione deterministica dell'intero genoma mediante siti di restrizione (drs-wga)
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4482979B1 (de) 2022-11-09 2025-10-01 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
WO2026083244A1 (en) 2024-10-14 2026-04-23 Menarini Silicon Biosystems S.P.A. Method for analysing the copy-number profile of a sample using deterministic restriction-site whole genome amplification (drs-wga)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science And Technology Corp., Kawaguchi Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
US6380370B1 (en) * 1997-08-14 2002-04-30 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to Staphylococcus epidermidis for diagnostics and therapeutics
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis

Also Published As

Publication number Publication date
EP1109938B1 (de) 2002-02-27
ES2172353T3 (es) 2002-09-16
CA2343359C (en) 2008-12-23
EP1109938A1 (de) 2001-06-27
AU755499B2 (en) 2002-12-12
NO20011316D0 (no) 2001-03-15
IL141749A0 (en) 2002-03-10
DK1109938T3 (da) 2002-05-27
NO20011316L (no) 2001-05-18
WO2000017390A1 (en) 2000-03-30
DE69900949T2 (de) 2002-10-02
JP4475488B2 (ja) 2010-06-09
DE69900949D1 (de) 2002-04-04
AU6192199A (en) 2000-04-10
CA2343359A1 (en) 2000-03-30
IL141749A (en) 2006-08-20
JP2002526087A (ja) 2002-08-20
US6673541B1 (en) 2004-01-06

Similar Documents

Publication Publication Date Title
ATE213783T1 (de) Dns amplifizierung aus einzelnen zellen
Vosberg The polymerase chain reaction: an improved method for the analysis of nucleic acids
CN101056883B (zh) 包括含有嵌入性假核苷酸(ipn)的嵌入性核酸(ina)的扩增阻断剂
DE69931646D1 (de) Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden
DE69435103D1 (de) Methode zur Bildung einer subtrahierten cDNA Bank und ihre Verwendung
CA2396778A1 (en) Methods and kits for identifying elite event gat-zm1 in biological samples
JP6100933B2 (ja) アレリックラダー遺伝子座
WO2000043531A2 (en) Methods and kits for characterizing gc-rich nucleic acid sequences
AU773854B2 (en) Method for the detection and/or analysis, by means of primer extension techniques, of single nucleotide polymorphisms in restriction fragments, in particular in amplified restriction fragments generated using AFLP
WO2005118847A1 (en) Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
EP0509089A1 (de) Verbindungen und methoden zur analyse von genomischen variationen
EP2488668B1 (de) Neue menschliche einzelnukleotid-polymorphismen
WO2002061123A3 (en) Method of analysing dna methylation using fluorescence polarisation
EP0787806A3 (de) ABO glycosyltransferase Sequenz Polymorphismus
CA2518904A1 (en) Molecular haplotyping of genomic dna
Akbas et al. AB0 blood subgroup allele frequencies in the Turkish population
AU699308B2 (en) Microsatellite sequences for canine genotyping
Tjensvoll et al. Haplotype analysis of Norwegian and Swedish patients with acute intermittent porphyria (AIP): Extreme haplotype heterogeneity for the mutation R116W
Matsushita et al. Genetic analysis of 18 STR loci on the X chromosome in a Japanese population
Vallian et al. Genotyping of five polymorphic STR loci in Iranian province of Isfahan
Saam et al. Improved breeding in camelids through molecular techniques: Development of STR multiplexes for identification and parentage verification
Ago et al. Highly polymorphic STR marker amplified with human DYS389 primers in Japanese macaques (Macaca fuscata)
Kubat et al. Short tandem repeat polymorphism at the HUMCD4 and HUMF13B loci in a Croatian population
Berschick et al. Analysis of the short tandem repeat polymorphism D18S51: Allele frequencies and sequence studies
CA2009870A1 (en) Metod for determining the origin of a human dna-containing sample