ATE215696T1 - Chemilumineszenz-assay mittels energietransfer - Google Patents
Chemilumineszenz-assay mittels energietransferInfo
- Publication number
- ATE215696T1 ATE215696T1 AT95906704T AT95906704T ATE215696T1 AT E215696 T1 ATE215696 T1 AT E215696T1 AT 95906704 T AT95906704 T AT 95906704T AT 95906704 T AT95906704 T AT 95906704T AT E215696 T1 ATE215696 T1 AT E215696T1
- Authority
- AT
- Austria
- Prior art keywords
- energy transfer
- chemiluminescence assay
- attophos
- biopolymer
- assays
- Prior art date
Links
- 238000003556 assay Methods 0.000 title abstract 3
- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical class C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- BTKMJKKKZATLBU-UHFFFAOYSA-N [2-(1,3-benzothiazol-2-yl)-1,3-benzothiazol-6-yl] dihydrogen phosphate Chemical compound C1=CC=C2SC(C3=NC4=CC=C(C=C4S3)OP(O)(=O)O)=NC2=C1 BTKMJKKKZATLBU-UHFFFAOYSA-N 0.000 abstract 2
- 229920001222 biopolymer Polymers 0.000 abstract 2
- 238000004166 bioassay Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D321/00—Heterocyclic compounds containing rings having two oxygen atoms as the only ring hetero atoms, not provided for by groups C07D317/00 - C07D319/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17227793A | 1993-12-23 | 1993-12-23 | |
| PCT/US1994/014866 WO1995017672A1 (en) | 1993-12-23 | 1994-12-23 | Chemiluminescent energy transfer assays |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE215696T1 true ATE215696T1 (de) | 2002-04-15 |
Family
ID=22627031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95906704T ATE215696T1 (de) | 1993-12-23 | 1994-12-23 | Chemilumineszenz-assay mittels energietransfer |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US5753436A (de) |
| EP (1) | EP0736174B1 (de) |
| JP (1) | JPH09507571A (de) |
| AT (1) | ATE215696T1 (de) |
| AU (1) | AU1518195A (de) |
| CA (1) | CA2179726A1 (de) |
| DE (1) | DE69430322T2 (de) |
| WO (1) | WO1995017672A1 (de) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0275260B1 (de) * | 1986-07-24 | 1993-11-18 | Tropix, Inc. | Verfahren zum nachweis einer verbindung unter verwendung des enzymatisch induzierten zerfalls von dioxethanen |
| US6022964A (en) | 1989-07-17 | 2000-02-08 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
| JPH11500222A (ja) * | 1995-02-13 | 1999-01-06 | トロピックス・インコーポレーテッド | 化学ルミネセンスエネルギー移動アッセイ |
| US5679803A (en) * | 1995-10-25 | 1997-10-21 | Tropix, Inc. | 1,2 chemiluminescent dioxetanes of improved performance |
| US6243980B1 (en) | 1997-03-07 | 2001-06-12 | Tropix, Inc. | Protease inhibitor assay |
| US6660529B2 (en) * | 1998-07-28 | 2003-12-09 | Pe Corporation | Heteroaryl substituted benzothiazole dioxetanes |
| WO2000006164A1 (en) * | 1998-07-28 | 2000-02-10 | Tropix, Inc. | Benzothiazole dioxetanes |
| US7166475B2 (en) * | 1999-02-26 | 2007-01-23 | Cyclacel Ltd. | Compositions and methods for monitoring the modification state of a pair of polypeptides |
| JP2002541858A (ja) * | 1999-04-16 | 2002-12-10 | ザイメテックス インコーポレイテッド | ウイルス由来の酵素および化学発光基質を用いるウイルス検出法 |
| US6686171B2 (en) * | 1999-05-10 | 2004-02-03 | Tropix, Inc. | Competitive chemiluminescent assay for cyclic nucleotide monophosphates |
| US7160996B1 (en) | 1999-06-09 | 2007-01-09 | Bioresearch Technologies, Inc. | Fluorescence energy transfer probes with stabilized conformations |
| WO2000075378A1 (en) * | 1999-06-09 | 2000-12-14 | Biosearch Technologies, Inc. | Fluorescence energy transfer probes with stabilized conformations |
| USD455817S1 (en) | 2001-05-03 | 2002-04-16 | Huang-Fu Huang | Water pistol |
| US7368296B2 (en) * | 2002-01-17 | 2008-05-06 | Applied Biosystems | Solid phases optimized for chemiluminescent detection |
| US7276343B2 (en) | 2002-06-24 | 2007-10-02 | Fujirebio Inc. | Chemiluminescence enhancer |
| US20040259182A1 (en) * | 2003-06-17 | 2004-12-23 | Brooks Edwards | Arrays for chemiluminescent assays, methods of making the arrays and methods of detecting chemiluminescent emissions on solid supports |
| EP1644515A2 (de) * | 2003-06-23 | 2006-04-12 | NLCPharma, Inc. | Enzymatischer diagnostischer test für sars und andere viruserkrankungen |
| US20050026151A1 (en) * | 2003-07-17 | 2005-02-03 | Voyta John C. | Simultaneous generation of multiple chemiluminescent signals on solid supports |
| US20050019778A1 (en) * | 2003-07-17 | 2005-01-27 | Voyta John C. | Sequential generation of multiple chemiluminescent signals on solid supports |
| WO2006076034A2 (en) * | 2004-05-21 | 2006-07-20 | Bhanu, Kalra | Stabilized two component system for chemilumiescent assay in immunodiagnostics |
| JP5073491B2 (ja) | 2004-07-19 | 2012-11-14 | プロテインシンプル | 分析対象物の検出のための方法 |
| US7846676B2 (en) | 2004-07-19 | 2010-12-07 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
| US7935479B2 (en) | 2004-07-19 | 2011-05-03 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
| WO2006116687A2 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanoassays |
| WO2006116683A1 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescence |
| WO2006116686A2 (en) * | 2005-04-27 | 2006-11-02 | The Trustees Of The University Of Pennsylvania | Nanostructure enhanced luminescent devices |
| US20070037174A1 (en) * | 2005-08-12 | 2007-02-15 | Rampal Jang B | Chemiluminescent generated fluorescent labeling |
| US8945361B2 (en) * | 2005-09-20 | 2015-02-03 | ProteinSimple | Electrophoresis standards, methods and kits |
| JPWO2007142314A1 (ja) * | 2006-06-08 | 2009-10-29 | 富士レビオ株式会社 | 発光増幅剤 |
| US9766206B2 (en) | 2013-09-27 | 2017-09-19 | ProteinSimple | Apparatus, systems, and methods for capillary electrophoresis |
| US20150093757A1 (en) | 2013-09-27 | 2015-04-02 | Protein Simple | Methods. apparatus and systems for detection of total protein using capillary electrophoresis |
| CN113567525B (zh) | 2016-01-13 | 2025-01-17 | 普诺森公司 | 用于毛细管电泳、等电点和分子量分析的系统和方法 |
| CA3068437A1 (en) | 2017-06-27 | 2019-01-03 | The Regents Of The University Of California | Mhc-i genotype restricts the oncogenic mutational landscape |
| US12163918B1 (en) | 2018-08-23 | 2024-12-10 | ProteinSimple | Compositions and methods for protein electrophoresis |
| US11782023B2 (en) | 2018-12-19 | 2023-10-10 | Regeneron Pharmaceuticals, Inc. | Ce-western applications for antibody development |
| BR112021023206A2 (pt) | 2019-05-21 | 2022-01-04 | Regeneron Pharma | Métodos para identificar e quantificar proteína de célula hospedeira |
| US11420202B1 (en) | 2021-08-31 | 2022-08-23 | ProteinSimple | Systems and methods for fractionation and collection of analytes in a sample |
| US20230075533A1 (en) | 2021-09-03 | 2023-03-09 | ProteinSimple | Methods and systems for analysis of samples containing particles used for gene delivery |
| US20240353409A1 (en) | 2023-04-14 | 2024-10-24 | Revivicor, Inc. | Novel serology assay for the detection of porcine viruses |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5004565A (en) * | 1986-07-17 | 1991-04-02 | The Board Of Governors Of Wayne State University | Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes |
| US4983779A (en) * | 1986-07-17 | 1991-01-08 | The Board Of Governors Of Wayne State University | Process for the preparation of vinyl ethers |
| US5112960A (en) * | 1989-07-17 | 1992-05-12 | Bronstein Irena Y | Chemiluminescent 3-(substituted adamant-2'-ylidene) 1,2-dioxetanes |
| US4931223A (en) * | 1986-07-24 | 1990-06-05 | Tropix, Inc. | Methods of using chemiluminescent 1,2-dioxetanes |
| US4956477A (en) * | 1987-12-31 | 1990-09-11 | Tropix, Inc. | Synthesis of 1,2-dioxetanes |
| CA1340590C (en) * | 1986-07-24 | 1999-06-08 | John C. Voyta | Chemiluminescence enhancement |
| US5220005A (en) * | 1986-07-24 | 1993-06-15 | Tropix, Inc. | Substituted adamantyl dioxetanes |
| US5177241A (en) | 1987-12-31 | 1993-01-05 | Bronstein Irena Y | Synthesis of 1,2-dioxetanes and intermediates therefor |
| US5639907A (en) | 1986-07-24 | 1997-06-17 | Tropix, Inc. | Synthesis of 1,2-dioxetanes and intermediates therefor |
| US4978614A (en) * | 1988-10-26 | 1990-12-18 | Tropix, Inc. | Method of detecting a substance using enzymatically-induced decomposition of dioxetanes |
| US5538847A (en) * | 1989-07-17 | 1996-07-23 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
| US5582980A (en) * | 1989-07-17 | 1996-12-10 | Tropix, Inc. | Chemiluminescent 1,2-dioxetanes |
| US5856522A (en) | 1995-05-04 | 1999-01-05 | Tropix, Inc. | Method of using synthesis of 1,2-dioxetanes and kits therefore |
| US5679802A (en) | 1989-07-17 | 1997-10-21 | Tropix, Inc. | 1,2-dioxetanes useful in chemiluminescent immunoassays |
| US5625077A (en) | 1987-12-31 | 1997-04-29 | Tropix, Inc. | 1,2-dioxetanes |
| US5089630A (en) * | 1987-12-31 | 1992-02-18 | Bronstein Irena Y | Dioxetanes for use in assays |
| US5756770A (en) | 1987-12-31 | 1998-05-26 | Tropix, Inc. | Synthesis of stable, water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
| US4952707A (en) * | 1988-06-30 | 1990-08-28 | Tropix, Inc. | Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes |
| JP2984282B2 (ja) * | 1989-06-27 | 1999-11-29 | トロピックス・インコーポレーテッド | エンハンサーを用いる化学発光測定法 |
| US5362916A (en) | 1989-09-06 | 1994-11-08 | Tropix, Inc. | Synthesis of mercaptaryl or hydroxyaryl enol ether alkali metal salts |
| US5225584A (en) | 1989-09-06 | 1993-07-06 | Tropix, Inc. | Synthesis of stable water-soluble chemiluminescent 1,2-dioxetanes and intermediates therefor |
| US5208148A (en) * | 1990-12-07 | 1993-05-04 | Molecular Probes, Inc. | Lipophilic fluorescent glycosidase substrates |
| US5336596A (en) * | 1991-12-23 | 1994-08-09 | Tropix, Inc. | Membrane for chemiluminescent blotting applications |
| US5679803A (en) * | 1995-10-25 | 1997-10-21 | Tropix, Inc. | 1,2 chemiluminescent dioxetanes of improved performance |
-
1994
- 1994-12-23 JP JP51762995A patent/JPH09507571A/ja active Pending
- 1994-12-23 CA CA002179726A patent/CA2179726A1/en not_active Abandoned
- 1994-12-23 EP EP95906704A patent/EP0736174B1/de not_active Expired - Lifetime
- 1994-12-23 AT AT95906704T patent/ATE215696T1/de active
- 1994-12-23 DE DE69430322T patent/DE69430322T2/de not_active Expired - Lifetime
- 1994-12-23 AU AU15181/95A patent/AU1518195A/en not_active Abandoned
- 1994-12-23 WO PCT/US1994/014866 patent/WO1995017672A1/en not_active Ceased
-
1995
- 1995-03-09 US US08/401,285 patent/US5753436A/en not_active Expired - Lifetime
-
1997
- 1997-03-04 US US08/811,539 patent/US5849495A/en not_active Expired - Lifetime
-
1998
- 1998-11-12 US US09/190,181 patent/US6287767B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09507571A (ja) | 1997-07-29 |
| DE69430322D1 (de) | 2002-05-08 |
| EP0736174A1 (de) | 1996-10-09 |
| CA2179726A1 (en) | 1995-06-29 |
| AU1518195A (en) | 1995-07-10 |
| US6287767B1 (en) | 2001-09-11 |
| EP0736174A4 (de) | 1999-07-28 |
| WO1995017672A1 (en) | 1995-06-29 |
| DE69430322T2 (de) | 2002-11-14 |
| EP0736174B1 (de) | 2002-04-03 |
| US5849495A (en) | 1998-12-15 |
| US5753436A (en) | 1998-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE69430322D1 (de) | Chemilumineszenz-assay mittels energietransfer | |
| AU3664589A (en) | Method and compositions providing enhanced chemiluminescence from 1,2-dioxetanes | |
| AU582293B2 (en) | Heterogeneous specific binding assay employing an aggregatable binding reagent | |
| DE69432709D1 (de) | Nukleinsäure - Sandwichassay in Lösungsphase mit reduzierten Hintergrundsignalen | |
| ATE212722T1 (de) | Verbesserte emission bei chemilumineszenzassays | |
| NZ306051A (en) | Testing using electrochemiluminescence | |
| NO864709D0 (no) | Gramnegativ bakteriuri-paavisning. | |
| CA2205135A1 (en) | Novel 1,2-dioxetane compounds with haloalkoxy groups, methods of preparation and use | |
| EP0276152A3 (de) | Immunoassays und Vorrichtungen gekennzeichnet durch orthographischen Fluss | |
| NO941781L (no) | Ikke-radioaktiv immunanalyse | |
| NO20003485L (no) | Kjemiluminiserende dioksetanderivater samt fremgangsmÕte for deteksjon | |
| DE69528375D1 (de) | Chemilumineszente dialkylsubstituierte 1,2-dioxetanverbindungen, synthesemethode und anwendung | |
| EP0256932A3 (en) | Use of oxidase enzyme systems in chemiluminescent assays | |
| GR910300015T1 (en) | Chemiluminescent 1,2-dioxetane compounds | |
| KR920004839A (ko) | 1종 이상의 분석물을 검출하기 위한 동시 검정법 | |
| ES8507692A1 (es) | Un procedimiento para preparar un trazador para uso en ensayo no isotopico | |
| KR890015714A (ko) | 폐암의 조기 검출 방법 | |
| AU7958091A (en) | Method for detecting and/or assaying hormones and antibodies for use in said method | |
| AU2002245206A1 (en) | Dendritic chemiluminescent substrates | |
| FI906044A0 (fi) | Buffrad tvaettmedelskomposition oloesliggoerande komposition, testmedel och anvaendningsfoerfarande. | |
| ES2070129T3 (es) | Ensayo de aglutinacion. | |
| IL96115A0 (en) | Enzymatic assay and assay kits for detecting and/or measuring the activation of cells | |
| ES2076221T3 (es) | Derivados geminales de difenilo y su uso en inmunoensayos. | |
| ES539598A1 (es) | Un procedimiento para preparar un trazador cromogeno a base de fluoresceinamina conjugada con l-tiroxina | |
| GB2335038A (en) | Double label immunoassay |