ATE232242T1 - Universale primersequenz zur multiplex dna amplifikation - Google Patents

Universale primersequenz zur multiplex dna amplifikation

Info

Publication number
ATE232242T1
ATE232242T1 AT96919243T AT96919243T ATE232242T1 AT E232242 T1 ATE232242 T1 AT E232242T1 AT 96919243 T AT96919243 T AT 96919243T AT 96919243 T AT96919243 T AT 96919243T AT E232242 T1 ATE232242 T1 AT E232242T1
Authority
AT
Austria
Prior art keywords
dna
primer sequence
present
dna amplification
universal primer
Prior art date
Application number
AT96919243T
Other languages
English (en)
Inventor
Anthony P Shuber
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23883585&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE232242(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Application granted granted Critical
Publication of ATE232242T1 publication Critical patent/ATE232242T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT96919243T 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation ATE232242T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/474,450 US5882856A (en) 1995-06-07 1995-06-07 Universal primer sequence for multiplex DNA amplification
PCT/US1996/009637 WO1996041012A1 (en) 1995-06-07 1996-06-06 Universal primer sequence for multiplex dna amplification

Publications (1)

Publication Number Publication Date
ATE232242T1 true ATE232242T1 (de) 2003-02-15

Family

ID=23883585

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96919243T ATE232242T1 (de) 1995-06-07 1996-06-06 Universale primersequenz zur multiplex dna amplifikation

Country Status (7)

Country Link
US (2) US5882856A (de)
EP (1) EP0832290B1 (de)
JP (1) JPH11507226A (de)
AT (1) ATE232242T1 (de)
CA (1) CA2223729C (de)
DE (1) DE69626111T2 (de)
WO (1) WO1996041012A1 (de)

Families Citing this family (136)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
US5814498A (en) * 1996-04-29 1998-09-29 Archer Daniels Midland Company Process for the recovery of organic acids and ammonia from their salts
US6294066B1 (en) 1997-01-23 2001-09-25 Archer Daniels Midland Company Apparatus and process for electrodialysis of salts
EP2327797B1 (de) 1997-04-01 2015-11-25 Illumina Cambridge Limited Verfahren zur Vervielfältigung von Nukleinsäuren
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
US6818404B2 (en) * 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
CA2307177C (en) 1997-10-23 2004-06-29 Exact Laboratories, Inc. Methods for detecting contamination in molecular diagnostics using pcr
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
WO1999058721A1 (en) * 1998-05-12 1999-11-18 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
DE19834948A1 (de) * 1998-08-03 2000-02-17 Monika Jecht Verfahren zur spezifischen Detektion von RNA
US7135284B1 (en) * 1999-02-05 2006-11-14 Integrated Dna Technologies, Inc. Primer extension methods for production of high specific activity nucleic acid probes
US6125531A (en) * 1999-03-01 2000-10-03 International Business Machines Corporation Method of making a printed circuit board having filled holes and a fill member for use therewith including reinforcement means
CA2366459A1 (en) * 1999-03-26 2000-10-05 Affymetrix, Inc. Universal arrays
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
EP1257664A4 (de) * 2000-01-28 2006-04-05 Althea Technologies Inc Verfahren zur analyse der genexpression
US20030219765A1 (en) * 2000-03-23 2003-11-27 Jose Costa Methods for evaluating cancer risk
EP2351853A1 (de) 2000-06-06 2011-08-03 Life Technologies Corporation Methode und Vorrichtungen für multiplex Amplifizierungsreaktionen
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
DE10065814B4 (de) * 2000-12-22 2004-07-08 Epigenomics Ag Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung
US6867021B2 (en) 2001-02-20 2005-03-15 Board Of Trustees Of Michigan State University Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli
US20060068433A1 (en) * 2004-09-20 2006-03-30 Godfrey Tony E Multiple mode multiplex reaction quenching method
CA2439402A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
WO2002090561A1 (en) * 2001-05-07 2002-11-14 Curagen Corporation Methods and composition for nucleic acid amplification
US6872529B2 (en) * 2001-07-25 2005-03-29 Affymetrix, Inc. Complexity management of genomic DNA
US7297778B2 (en) * 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20090065471A1 (en) * 2003-02-10 2009-03-12 Faris Sadeg M Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
CA2460639C (en) 2001-09-19 2013-07-16 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
US7414111B2 (en) 2001-09-19 2008-08-19 Alexion Pharmaceuticals, Inc. Engineered templates and their use in single primer amplification
WO2003033741A1 (en) * 2001-10-16 2003-04-24 Aclara Biosciences, Inc. Universal e-tag primer and probe compositions and methods
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
CA2467460A1 (en) * 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
WO2003060159A2 (en) * 2002-01-15 2003-07-24 Matforsk Methods of nucleic acid amplification
RU2215037C1 (ru) * 2002-04-15 2003-10-27 Институт биоорганической химии им. М.М. Шемякина и Ю.А.Овчинникова РАН Способ ферментативного синтеза днк
US7176002B2 (en) * 2002-05-16 2007-02-13 Applera Corporation Universal-tagged oligonucleotide primers and methods of use
WO2003097828A1 (fr) * 2002-05-21 2003-11-27 Adgene Co., Ltd. Procede d'identification d'acides nucleiques
EP1552010B1 (de) * 2002-07-19 2010-06-09 Althea Technologies, Inc. Strategien zur genexpressionsanalyse
FR2842534B1 (fr) * 2002-07-19 2006-01-20 Inst Nat Sante Rech Med Amplification multiplex quantitative a l'echelle d'un genome, et applications a la detection de remaniements genomiques
AU2003254298A1 (en) 2002-08-02 2004-02-23 Stratatech Corporation Species specific dna detection
CA2410795A1 (en) * 2002-11-01 2004-05-01 University Of Ottawa A method for the amplification of multiple genetic targets
DE10253337B4 (de) * 2002-11-14 2005-10-20 November Ag Molekulare Medizin Verfahren zum Nachweis einer Nukleinsäure
EP1594975A4 (de) * 2002-12-04 2006-08-02 Applera Corp Multiplex-amplifikation von polynukleotiden
AU2003202671A1 (en) * 2003-01-21 2004-08-13 Guoliang Fu Quantitative multiplex detection of nucleic acids
WO2004065628A1 (en) * 2003-01-21 2004-08-05 Guoliang Fu Quantitative multiplex detection of nucleic acids
JP4280519B2 (ja) * 2003-03-07 2009-06-17 アークレイ株式会社 Ｄｎａ増幅法およびそのためのキット
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
CA2528577C (en) * 2003-06-20 2012-08-07 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7824856B2 (en) * 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
EP1687437A4 (de) * 2003-10-13 2008-01-02 Genaco Biomedial Products Inc Verfahren und kit zur auf primern beruhenden amplifikation von nukleinsäuren
US7824857B2 (en) * 2003-12-02 2010-11-02 Musc Foundation For Research Development Methods and compositions for diagnosing epithelial cell cancer
US7981616B2 (en) * 2004-02-27 2011-07-19 Musc Foundation For Research Development Enhanced detection of RNA using a panel of truncated gene-specific primers for reverse transcription
EP1721011A1 (de) * 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
EP1591534A1 (de) * 2004-04-01 2005-11-02 Stichting Sanquin Bloedvoorziening Verfahren zur Genotypisierung von Antigenen von Blutzellen und ein Kit zur Genotypisierung
SE0401270D0 (sv) 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
WO2006085964A2 (en) 2004-06-30 2006-08-17 Applera Corporation Log-linear amplification
WO2006017150A2 (en) * 2004-07-09 2006-02-16 University Of Pittsburgh - Of The Commonwealth System Of Higher Educatiion Identification of markers in lung and breast cancer
CN1280422C (zh) * 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
EP1802767A2 (de) * 2004-09-22 2007-07-04 University Of The West Of England, Bristol Rhd- und abo-gentypisierung mit multiplex-pcr
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060199188A1 (en) * 2005-03-03 2006-09-07 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
EP1984738A2 (de) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren
EP4190448A3 (de) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtungen
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US8198027B2 (en) 2006-12-21 2012-06-12 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
CA2677771A1 (en) * 2007-03-01 2008-09-04 Symphogen A/S Method for cloning cognate antibodies
CA2984820C (en) * 2007-04-04 2021-12-07 Ande Corporation Plastic microfluidic separation and detection platforms
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
CA2700233A1 (en) * 2007-10-22 2009-04-30 Monoquant Pty Ltd. A method of dna amplification
CN104928335A (zh) * 2008-04-03 2015-09-23 哈森阿尔法生物技术研究院 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
WO2010057525A1 (en) * 2008-11-19 2010-05-27 Fondazione Parco Tecnologico Padano Oligonucleotide primers for nucleotide indexing of polymorphic pcr products and methods for their use
US8528589B2 (en) 2009-03-23 2013-09-10 Raindance Technologies, Inc. Manipulation of microfluidic droplets
WO2010123625A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms predict clinical outcome in patients with cancer
WO2010147654A2 (en) 2009-06-15 2010-12-23 Netbio Inc. Improved methods for forensic dna quantitation
EP2449132B1 (de) 2009-07-01 2015-05-13 Gen-Probe Incorporated Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
EP2287334A1 (de) * 2009-08-21 2011-02-23 Qiagen GmbH Verfahren zum Nachweis von Zielnukleinsäuren
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
US20120288861A1 (en) 2009-12-21 2012-11-15 Heinz-Josef Lenz Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP4435111A1 (de) 2010-02-12 2024-09-25 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
WO2012032510A1 (en) 2010-09-07 2012-03-15 Yeda Research And Development Co. Ltd. Primers for amplifying dna and methods of selecting same
WO2012045012A2 (en) 2010-09-30 2012-04-05 Raindance Technologies, Inc. Sandwich assays in droplets
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
EP2675819B1 (de) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
WO2012122571A1 (en) * 2011-03-10 2012-09-13 Gen-Probe Incorporated Methods and compositions for the selection and optimization of oligonucleotide tag sequences
US20130059738A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
EP2702170B1 (de) 2011-04-28 2015-12-16 Life Technologies Corporation Verfahren und zusammensetzungen für multiplex-pcr
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
JP5299981B1 (ja) * 2011-09-08 2013-09-25 独立行政法人理化学研究所 プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
BR112014019168B1 (pt) 2012-02-03 2021-10-05 California Institute Of Technology Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
US9139870B2 (en) 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
GB201316644D0 (en) 2013-09-19 2013-11-06 Kymab Ltd Expression vector production & High-Throughput cell screening
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US10557650B2 (en) 2014-07-23 2020-02-11 Biotech Trentino S.P.A. Apparatus for the cooling of a drinking liquid, in particular drinking water, with innovative cooling system with peltier effect
JP6963505B2 (ja) 2015-03-25 2021-11-10 アングル ヨーロッパ リミテッド 鎖置換ポリメラーゼを用いた固相核酸標的捕捉及び複製
US11655510B2 (en) * 2015-04-20 2023-05-23 Cellecta, Inc. Experimentally validated sets of gene specific primers for use in multiplex applications
EP3286332A4 (de) 2015-04-20 2018-12-12 Cellecta, Inc. Experimentell validierte sets genspezifischer primer für multiplex-anwendungen
CN107849600A (zh) 2015-06-09 2018-03-27 生命技术公司 用于分子标记的方法、系统、组合物、试剂盒、装置和计算机可读媒体
CN106795569B (zh) * 2015-07-07 2017-12-05 上海真固生物科技有限公司 减少引物二聚体扩增的方法
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
GB201621477D0 (en) * 2016-12-16 2017-02-01 Multiplicom Nv Modified multiplex and multistep amplification reactions and reagents therefor
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
US12522856B2 (en) 2018-09-11 2026-01-13 The Board Of Trustees Of The Leland Stanford Junior University Methods and systems for multiplex gene amplification from ultra-low DNA input amounts and uses thereof
US20220307093A1 (en) 2019-07-03 2022-09-29 Gen-Probe Incorporated Oligonucleotides for use in determining the presence of trichomonas vaginalis in a sample
WO2021126997A1 (en) * 2019-12-18 2021-06-24 Petdx, Inc. Methods and compositions for cancer detection, characterization or management in companion animals
CN114717298A (zh) 2021-01-06 2022-07-08 上海慧众同康生物科技有限公司 一种多重扩增方法
WO2025240660A1 (en) 2024-05-15 2025-11-20 Foresite Labs, Llc Drug development and drug activity determination for coronary artery disease (cad)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2538367B2 (ja) * 1988-06-24 1996-09-25 ブリティッシュ・テクノロジー・グループ・リミテッド Ｆｏｗｌｐｏｘウィルスの非必須領域
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
WO1993018177A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia Diagnosis of cystic fibrosis using allele specific multiplex polymerase chain reactions
WO1993018178A1 (en) * 1992-03-13 1993-09-16 The Children's Hospital Of Philadelphia DIAGNOSIS OF β-THALASSEMIA USING A MULTIPLEX AMPLIFICATION REFRACTORY MUTATION SYSTEM
US5474896A (en) * 1992-05-05 1995-12-12 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
US5518901A (en) * 1993-04-19 1996-05-21 Murtagh; James J. Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
DK172394B1 (da) * 1994-02-25 1998-05-18 Micro Matic As Dobbeltventil
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification

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AU703417B2 (en) 1999-03-25
CA2223729A1 (en) 1996-12-19
EP0832290B1 (de) 2003-02-05
CA2223729C (en) 2004-04-20
JPH11507226A (ja) 1999-06-29
US6207372B1 (en) 2001-03-27
DE69626111T2 (de) 2003-12-24
WO1996041012A1 (en) 1996-12-19
EP0832290A1 (de) 1998-04-01
DE69626111D1 (de) 2003-03-13
AU6163696A (en) 1996-12-30
US5882856A (en) 1999-03-16

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